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Microbial degradation of polyhydroxyalkanoates in tropical soils / A. N. Boyandin [et al.] // International Biodeterioration and Biodegradation. - 2013. - Vol. 83. - P77-84, DOI 10.1016/j.ibiod.2013.04.014 . - ISSN 0964-8305
Кл.слова (ненормированные):
Biopolymer properties -- Biopolymers -- PHA degrading microorganisms -- Polyhydroxyalkanoates (PHAs) -- Soil biodegradation -- 3-hydroxybutyric acid -- Degree of crystallinity -- Hydroxyalkanoic acids -- Microbial characteristics -- Microbial degradation -- Polyhydroxyalkanoates -- Polymer molecular mass -- Soil biodegradations -- Bacteriology -- Biomolecules -- Biopolymers -- Microorganisms -- Soils -- Tropics -- Biodegradation -- biodegradation -- chemical composition -- microbial activity -- microbial community -- molecular analysis -- organic compound -- polymer -- soil degradation -- soil microorganism -- tropical soil
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. В© 2013 Elsevier Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russian Federation
The Joint Russian-Vietnam Tropical Research and Test Center, Hanoi, Viet Nam
Inst. of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Prudnikova, S.V.; Karpov, V.A.; Ivonin, V.N.; D?, N.L.; Nguy?n, T.H.; Le, T.M.H.; Filichev, N.L.; Levin, A.L.; Filipenko, M.L.; Volova, T.G.; Gitelson, I.I.

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The physicochemical properties of polyhydroxyalkanoates with different chemical structures / T. G. Volova [et al.] // Polymer Science - Series A. - 2013. - Vol. 55, Is. 7. - P427-437, DOI 10.1134/S0965545X13070080 . - ISSN 0965-545X
Кл.слова (ненормированные):
Amorphous and crystalline phasis -- Degrees of crystallinity -- Physicochemical property -- Poly(3-hydroxybutirate) -- Polyhydroxyalkanoate copolymer -- Temperature characteristic -- Thermal degradation temperatures -- Thermoplastic materials -- Copolymers -- Monomers -- Thermoplastic elastomers -- Structure (composition)
Аннотация: A set of polyhydroxyalkanoates are synthesized, and a comparative study of their physicochemical properties is performed. The molecular masses and polydispersities of polyhydroxyalkanoates are found to be independent of their chemical structures. It is shown that the temperature characteristics and degrees of crystallinity of polyhydroxyalkanoates are affected by the chemical compositions of the monomers and their quantitative contents in the polymers. The incorporation of 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate units into the chain of poly(3-hydroxybutyrate) decreases its melting point and thermal degradation temperature relative to these parameters of a homogeneous poly(3-hydroxybutyrate) sample (175 В± 5 C and 275 В± 5 C, respectively). The higher the content of the second monomer units in the poly(3-hydroxybutirate) chain, the greater the changes. The degrees of crystallinity of polyhydroxyalkanoate copolymers are generally lower than that of poly(3-hydroxybutyrate) (75 В± 5%). The effect on the ratio of the amorphous and crystalline phases of the copolymer samples becomes more pronounced in the series 3-hydroxy-valerate-3-hydroxyhexanoate-4- hydroxybutyrate. The prepared samples exhibit different properties ranging from rigid thermoplastic materials to engineering elastomers. В© 2013 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, str. 50, Krasnoyarsk 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk 660041, Russian Federation
Siberian State Technological University, pr. Mira 82, Krasnoyarsk 660049, Russian Federation
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, str. 38, Krasnoyarsk 660036, Russian Federation
Massachusetts Institute of Technology, Cambridge, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Shishatskaya, E.I.; Mironov, P.V.; Vasil'Ev, A.D.; Sukovatyi, A.G.; Sinskey, A.J.

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An in vivo study of pha matrices of different chemical composition: Tissue reaction and biodegradation / E. I. Shishatskaya [et al.] // Cellular Transplantation and Tissue Engineering. - 2012. - Vol. 7, Is. 1. - P73-80 . - ISSN 1815-445X
Кл.слова (ненормированные):
Degradable polymer matrices -- Degradation -- PHAs -- Polyhydroxyalkanoates -- Subcutaneous implantation -- Tissue reaction
Аннотация: The study addresses consequences of subcutaneous implantation of film matrices prepared from different PHAs to laboratory animals. No negative effects of subcutaneous implantation of PHA matrices on physiological and biochemical characteristics of the animals were determined. Independently of the matrices composition and duration of the contact with the internal environment of the organism we did not observe any deviations in the behavior of animals, their growth and development, as well as blood functions. Response of the tissues to PHA matrices was comparable with the response to polylactide, but substantially less expressed at the earlier time periods after implantation. Tissues response to implantation of PHA of all types is characterized by short-term (up to 2 weeks) post-traumatic inflammation with formation of fibrous capsules by 30th-60th days with the thickness less than 100 microns, which get thinner down to 40-60 microns by 180th day as the result of involution. No differences in response of tissues and the whole organism were observed for the matrices produced from the homopolymer of 3-hydroxybutyric acid (P3HB), copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate acids (P3HB/3HH). Macrophages and foreign-body giant cells actively participate in the response of the tissues to PHAs. In the studied conditions matrices from the copolymers containing 3-hydroxyhexanoate and 4 hydroxybutyrate were determined as more actively degraded PHA. The next less degraded matrices were matrices from the copolymer of P3HB/3HV and the most resistant were P3HB matrices. The slower degradation of PHA matrices was accompanied by delayed development of giantcells response. The studied PHA matrices can be placed in the following range by their degradation: P3HB/3HH - P3HB/4HB - P3HB/HV - P3HB.

Scopus
Держатели документа:
Institute of Modern Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Massachusetts Institute of Technology, Cambridge, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Nikolaeva, E.D.; Goreva, A.V.; Brigham, C.J.; Volova, T.G.; Sinskey, A.J.

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Comparative investigation of polyhydroxyalkanoate scaffolds with various chemical compositions / E. D. Nikolaeva [и др.] // Cellular Transplantation and Tissue Engineering. - 2011. - Vol. 6, Is. 4. - С. 54-63 . - ISSN 1815-445X
Кл.слова (ненормированные):
Biopolymers -- Scaffolds -- Tissue engineering
Аннотация: The authors have constructed and characterized a series of membranes based on resorbable polyhydroxyalkanoates of different compositions. Five PHA types have been studied: a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids, 3-hydroxybutyric and 3-hydroxyvaleric acids, 3-hydroxybutyric and 3-hydroxyhexanoic acids. Scanning electron microscopy and atomicforce microscopy were used to examine the microstructure of membrane surfaces, showing that membranes based on the copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate had the roughest surface, while membranes based on the copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate had the smoothest surface. The contact angle for water in air was smaller and hydrophilic properties better in the copolymer membranes than in the membranes based on the high-crystallinity homopolymer of 3-hydroxybutyric acid. The culture of mouse fibroblast cell line NIH 3,3 was used to test PHAbased membranes; results of fluorescent probes of DNA DAPI and the MTT assay show that membranes based on studied PHAs are not cytotoxic on direct contact with cells and are highly biocompatible; their adhesive properties and ability to maintain fibroblast proliferation are similar to those of polystyrene and better than those of polylactic acid membranes.

Scopus
Держатели документа:
Institute of Biophisycs, SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russian Federation
Massachusetts Institute of Technology, Cambridge, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nikolaeva, E.D.; Shishatskaya, E.I.; Mochalov, K.E.; Volova, T.G.; Sinsky, A.J.

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Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 2011. - Vol. 47, Is. 5. - P494-499, DOI 10.1134/S0003683811050152 . - ISSN 0003-6838
Кл.слова (ненормированные):
Bacteria (microorganisms) -- Cupriavidus -- Cupriavidus necator
Аннотация: Synthesis of 3- and 4-hydroxybutyrate copolymer (3HB-co-4HB), the most promising member of the biodegradable polyhydroxyalkanoate (PHA) family, has been studied. Cultivation conditions of naturally occurring strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus B10646 have been optimized to ensure efficient synthesis of the 3HB-co-4HB copolymer. A set of highly pure samples of the 3HB-co-4HB copolymer with 4HB content varying from 8.7 to 24.3 mol% has been obtained. Incorporation of 4HB into the copolymer was shown to cause a more pronounced decrease in polymer crystallinity than the incorporation of 3-hydroxyvalerate or 3-hydroxyhexanoate; samples with a degree of crystallinity below 30% have been obtained. The weight average molecular mass of the 3HB-co-4HB copolymers was shown to be independent on the monomer ratio and to vary broadly (from 540 to 1110 kDa). В© 2010 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk 660041, Russian Federation
Biophysics Institute, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Chemistry and Chemical Technology Institute, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Massachusetts Institute of Technology, Cambridge, MA 02139, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Sokolenko, V.A.; Sinski, E.J.

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Biodegradation of polyhydroxyalkanoates (PHAs) in the South China Sea and identification of PHA-degrading bacteria / T. G. Volova [et al.] // Microbiology. - 2011. - Vol. 80, Is. 2. - P252-260, DOI 10.1134/S0026261711020184 . - ISSN 0026-2617
Кл.слова (ненормированные):
biodegradation in marine environments -- microbial PHA degraders -- PHA -- polyhydroxyalkanoates -- Bacillus (bacterium) -- Bacillus sp. -- Bacteria (microorganisms) -- Enterobacter -- Enterobacter cloacae -- Gracilibacillus -- Prospect Hill virus
Аннотация: The biodegradation patterns of two types of PHA, a 3-hydroxybutyrate (3-PHB) polymer and a 3-hydroxybutyrate and 3-hydroxyvalerate (3-PHB/3-PHV) copolymer, were studied in tropical marine environments (Dam Bay, South China Sea, Nha Trang, Vietnam). No reliable differences in the degradation of 3-PHB and 3-PHB/3-PHV were revealed. It was shown that the degradation process depended mainly on the shape of a polymer product and its production method: the degradation of polymer films was found to be more active than that of molded solids. A decrease in the molecular mass of both types of PHA was detected in the course of the degradation of PHA samples. However, the degree of PHA crystallinity did not change; that is, the levels of degradation of both the amorphous and crystalline phases of PHA were almost the same. Among microbial PHA degraders, three bacterial strains, Bacillus sp. IBP-V002, Enterobacter cloacae sp. IBP-V001, and Gracilibacillus sp. IBP-V003, were identified based on the results of morphological, biochemical, and molecular phylogenetic analyses. The ability of the representatives of the genera Gracilibacillus and Enterobacter to degrade PHA was revealed for the first time. В© 2011 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russian Federation
Marine Department, Joint Russian-Vietnamese Tropical Research and Test Center, Nha Trang, Viet Nam : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Boyandin, A.N.; Vasil'ev, A.D.; Karpov, V.A.; Kozhevnikov, I.V.; Prudnikova, S.V.; Rudnev, V.P.; Xuan, B.B.; Dung, V.V.; Gitel'zon, I.I.

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Biodegradation of polyhydroxyalkanoates (PHAs) in tropical coastal waters and identification of PHA-degrading bacteria / T. G. Volova [et al.] // Polymer Degradation and Stability. - 2010. - Vol. 95, Is. 12. - P2350-2359, DOI 10.1016/j.polymdegradstab.2010.08.023 . - ISSN 0141-3910
Кл.слова (ненормированные):
Biopolymers in marine environment -- Degradation of polymers -- PHA properties -- PHA-degrading microorganisms -- Polyhydroxyalkanoates, PHA -- 16S rRNA -- 3-hydroxybutyric acid -- Amorphous phase -- Bacillus sp -- Biodegradation rate -- Chemical compositions -- Coastal waters -- Degradation rate -- Degrading bacteria -- Degree of crystallinity -- Enterobacter -- Hydroxyvalerate -- Marine environment -- PHA properties -- PHA-degrading microorganisms -- Polydispersity indices -- Polyhydroxyalkanoates -- Polymer chains -- Preparation technique -- Sole carbon source -- South China Sea -- Viet Nam -- Acids -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Biomolecules -- Biopolymers -- Microorganisms -- Organic polymers -- Polydispersity -- Polymer films -- Polymers -- RNA -- Seawater -- Degradation
Аннотация: Biodegradability patterns of two PHAs: a polymer of 3-hydroxybutyric acid (3-PHB) and a copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids (3-PHB/3-PHV) containing 11 mol% of hydroxyvalerate, were studied in the tropical marine environment, in the South China Sea (Nha Trang, Vietnam). No significant differences have been observed between degradation rates of 3-PHB and 3-PHB/3-PHV specimens; it has been found that under study conditions, biodegradation is rather influenced by the shape of the polymer item and the preparation technique than by the chemical composition of the polymer. Biodegradation rates of polymer films in seawater have been found to be higher than those of compacted pellets. As 3-PHB and 3-PHB/3-PHV are degraded and the specimens lose their mass, molecular weight of both polymers is decreased, i.e. polymer chains get destroyed. The polydispersity index of the PHAs grows significantly. However, the degree of crystallinity of both PHAs remains unchanged, i.e. the amorphous phase and the crystalline one are equally disintegrated. PHA-degrading microorganisms were isolated using the clear-zone technique, by inoculating the isolates onto mineral agar that contained PHA as sole carbon source. Based on the 16S rRNA analysis, the PHA-degrading strains were identified as Enterobacter sp. (four strains), Bacillus sp. and Gracilibacillus sp.В© 2010 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, 79 Svobodny av., Krasnoyarsk 660041, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
A.N. Severtsov Institute of Ecology and Evolution RAS, 33 Leninskij Prospect, Moscow 119071, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 8 Lavrentiev Ave., Novosibirsk 630090, Russian Federation
Joint Russian-Vietnam Tropical Research and Test Centre, Coastal Branch, 30 Nguyen Thien Thuat, Nha Trang, Viet Nam : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Boyandin, A.N.; Vasiliev, A.D.; Karpov, V.A.; Prudnikova, S.V.; Mishukova, O.V.; Boyarskikh, U.A.; Filipenko, M.L.; Rudnev, V.P.; Ba Xuan, B.; Vit Dung, V.; Gitelson, I.I.

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Degradation of P(3HB) and P(3HB-co-3HV) in biological media / E. I. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - P643-657, DOI 10.1163/1568562053783678 . - ISSN 0920-5063
Кл.слова (ненормированные):
Degradation rate -- Fiber properties -- Morphology -- Poly(hydroxybutyrate-co-hydroxyvalerate) (P(3HB-co-3HV)) -- Polyhydroxyalkanoates(PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Copolymers -- Degradation -- Differential scanning calorimetry -- Enzymes -- Morphology -- Scanning electron microscopy -- Tensile strength -- Tissue -- Transmission electron microscopy -- Degradation rate -- Fiber properties -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Biopolymers -- buffer -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxybutyrate hydroxyvalerate copolymer -- unclassified drug -- animal experiment -- animal model -- animal tissue -- article -- biodegradation -- controlled study -- crystal structure -- fiber -- giant cell -- in vitro study -- in vivo study -- macrophage -- morphology -- nonhuman -- pH -- priority journal -- rat -- structure analysis -- tensile strength -- tissue water -- weight reduction -- Animals -- Biodegradation, Environmental -- Buffers -- Humans -- Hydrogen-Ion Concentration -- Hydroxybutyrates -- Macrophages -- Microscopy, Electron, Scanning -- Microscopy, Electron, Transmission -- Muscle, Skeletal -- Polyesters -- Rats -- Rats, Wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 600326, Russian Federation
Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G3, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gordeev, S.A.; Puzyr, A.P.

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Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures / E. I. Shishatskaya [et al.] // Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - P719-728, DOI 10.1023/B:JMSM.0000030215.49991.0d . - ISSN 0957-4530
Кл.слова (ненормированные):
Necrosis -- Polymeric implants -- Suppurative inflammation -- Tissue reaction -- Biodegradation -- Calcification (biochemistry) -- Cells -- Copolymers -- Implants (surgical) -- Silk -- Tissue -- Tumors -- Materials science -- acid phosphatase -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polyhydroxyvaleric acid -- unclassified drug -- animal experiment -- animal model -- article -- biodegradable implant -- blood vessel reactivity -- catgut -- controlled study -- enzyme activity -- female -- giant cell -- histochemistry -- inflammation -- macrophage -- nonhuman -- priority journal -- rat -- silk -- suture -- tensile strength -- tissue reaction -- tissue structure -- wound healing -- young modulus -- Absorbable Implants -- Animals -- Female -- Fibrosis -- Foreign-Body Reaction -- Hydroxybutyrates -- Muscle, Skeletal -- Polyesters -- Polymers -- Rats -- Rats, Wistar -- Sutures -- Treatment Outcome -- Wounds, Penetrating -- Animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.

Scopus
Держатели документа:
Inst. Biophys. Siberian Br. Russ. A., Akademgorodok, Krasnoyarsk 660036, Russia, Russian Federation
Terr. Pathological Anatomy Bureau, Partisan Zheleznyak St. 1, Krasnoyarsk 660049, Russia, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Puzyr, A.P.; Mogilnaya, O.A.; Efremov, S.N.

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10.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood / V. I. Sevastianov [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - P1029-1042, DOI 10.1163/156856203769231547 . - ISSN 0920-5063
Кл.слова (ненормированные):
?-hydroxy acids -- Endotoxins -- Hemocompatibility -- Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (PHB) -- bacterium lipopolysaccharide -- carbon -- complement -- copolymer -- hydroxyacid -- long chain fatty acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- valeric acid derivative -- adult -- article -- biofilm -- biotechnology -- blood analysis -- blood clotting -- blood compatibility -- cell function -- chemical analysis -- chemical composition -- complement activation -- concentration (parameters) -- controlled study -- gas chromatography -- hemostasis -- human -- human cell -- mass spectrometry -- micromorphology -- nonhuman -- priority journal -- purification -- quantitative analysis -- sampling -- synthesis -- thrombocyte adhesion -- Wautersia eutropha -- Biocompatible Materials -- Blood -- Blood Coagulation Tests -- Chromatography, Gas -- Complement Activation -- Cupriavidus necator -- Fatty Acids -- Humans -- Platelet Adhesiveness -- Polyesters -- Surface Properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

Scopus
Держатели документа:
Inst. of Transplantol. Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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11.

Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - P675-678, DOI 10.1007/s00253-002-0941-8 . - ISSN 0175-7598
Кл.слова (ненормированные):
3 hydroxybutyric acid -- acetoacetyl coenzyme a reductase -- acetyl coenzyme A acyltransferase -- beta hydroxyvalerate -- butyrate dehydrogenase -- carbon monoxide -- electrolyte -- hydrogen -- oxidoreductase -- poly(3 hydroxybutyric acid) -- poly(3 hydroxybutyric acid)synthase -- polyhydroxyalkanoic acid -- polymer -- unclassified drug -- valeric acid -- bacterium -- article -- autotrophy -- bacterial growth -- bacterial strain -- biomass production -- controlled study -- crystallization -- enzyme activity -- molecular weight -- nonhuman -- synthesis -- temperature -- Wautersia eutropha -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Lipids -- Polyesters -- Bacteria (microorganisms) -- Negibacteria -- Ralstonia -- Wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Br. Russian Academy of Sci., 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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12.

Study of the biological properties of polyoxyalkanoates in the in vivo chronic experiment / E. I. Shishatskaya, T. G. Volova, T. G. Popova // Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 29-33 . - ISSN 0025-8075
Кл.слова (ненормированные):
biomaterial -- polymer -- animal -- article -- bioremediation -- comparative study -- female -- metabolism -- rat -- suture -- Wistar rat -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Female -- Polymers -- Rats -- Rats, Wistar -- Sutures -- Biocompatibility -- Immunology -- Implants (surgical) -- Materials testing -- Organic polymers -- Polyoxyalkanoates -- Rat models -- Suture threads -- Biomaterials
Аннотация: Chronic experiments on mature Wistar rats studied the biological properties of suture threads from two types of polyoxyalkanoates (POA), such as polyoxybutyrate (POB) and the copolymer of POB and polyoxyvalerate (POV) (POB/POV) as compared to the conventional surgical material (silk and catgut). The physiological and biochemical parameters that reflect the status of the animals, including their behavior, height and development, the weight of the body and viscera, the morphology of peripheral blood, the biochemical parameters of blood, the activity of some enzymes, as well as histological findings of the viscera, and lymphoid tissue responses were assessed. POA was found to produce no negative effect on the physiological, biochemical, and functional characteristics of the animals irrespective of the duration of contact with the internal medium of the body and of the chemical composition of a material.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Popova, T.G.

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13.

The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Microbiology. - 2001. - Vol. 70, Is. 6. - P640-646 . - ISSN 0026-2617
Кл.слова (ненормированные):
CO inhibition -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates (PHAs) -- Synthesis
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen. В© 2001 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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14.

The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752 . - ISSN 0026-3656
Кл.слова (ненормированные):
CO inhibition -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates (PHAs) -- Synthesis -- alkane -- carbon monoxide -- fatty acid -- hydroxyacid -- lipid -- nitrogen -- polyester -- article -- chemistry -- culture medium -- drug antagonism -- growth, development and aging -- mass spectrometry -- metabolism -- Wautersia eutropha -- Alkanes -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Hydroxy Acids -- Lipids -- Mass Spectrometry -- Nitrogen -- Polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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15.

Cultivation of multipotent mesenchymal bone marrow cells on matrixes made of resorbable bioplastotan / E. I. Shishatskaya [и др.] // Cellular Transplantation and Tissue Engineering. - 2013. - Vol. 8, Is. 1. - С. 57-65 . - ISSN 1815-445X
Кл.слова (ненормированные):
Alkaline phosphatase -- Differentiation -- Extracellular calcium precipitates -- Multipotent mesenchymal stromal cells -- Osteopontin -- Resorbable bioplastotan
Аннотация: Series of cell carriers made of resorbable polyester Bioplastotan, which is a copolymer of 3- And 4-hydroxybutyric acid, are characterized in this work. With use of various technologies membranes, 3D scaffolds, and nanomatrixes formed by ultrafine fibers produced with electrostatic spinning were constructed. All types of scaffolds provide adhesion of multipotent mesenchymal stromal cells and are suitable for the cultivation and differentiation of cells in the osteoblastes, that confirmed by detection of extracellular calcium precipitates, alkaline phosphatase activity and osteopontin production in cell culture. В© Human stem cells institute, 2013.

Scopus
Держатели документа:
Siberian Branch, Institute of Biophisycs, RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Siberian Branch, L.V. Kirensky Institute of Physics, RAS, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Nikolaeva, E.D.; Shumilova, A.A.; Shabanov, A.V.; Volova, T.G.

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16.

Biomedical investigations of biodegradable PHAs / E. I. Shishatskaya // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P65-81, DOI 10.1002/masy.200850909 . - ISSN 1022-1360
Кл.слова (ненормированные):
Biodegradable polymers -- Biomedical investigations -- PHA -- ABS resins -- Biodegradable polymers -- Biopolymers -- Biotechnology -- Bone -- Cell culture -- Endothelial cells -- Fibers -- Functional polymers -- Osteoblasts -- Polymers -- Surgery -- Abdominal surgeries -- Biocompatible -- Biodegradable -- Biomedical -- Biomedical investigations -- Bone defects -- Ectopic bones -- Hepatocytes -- In vitro -- Microparticles -- Oral surgeries -- Osteogenesis -- PHA -- Polyhydroxybutyrate -- Polymer devices -- Ralstonia -- Russian academy of sciences -- Two types -- Polymer films
Аннотация: This work is a review of the results of biomedical studies of polymer devices (films, fibers, microparticles, 30 implants) made from resorbable PHAs synthesized by the bacterium Wautersia (Ralstonia) eutropha 65786, using the technology developed at the Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences. Two types of PHAs - polyhydroxybutyrate (PHB) and a hydroxybutyrate/hydroxyvalerate copolymer (PHB/PHV) - have been proven to be biocompatible in vitro in cultures of fibroblasts, endothelial cells, hepatocytes, and osteoblasts, and in short- and long-duration experiments on animals. Polymer films and membranes have been found to be usable as scaffolds for functioning cells and monofilament suture fibers - for stitching muscular-fascial wounds and in abdominal surgery. Ectopic bone formation assay and experiments with the model of segmental osteotomy showed that 30 PHB and PHB/HA implants can be used for reparative osteogenesis. The paper reports beneficial results of using polymers to repair bone defects in oral surgery. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodnui Av., 69, Krasnoyarsk, 660148, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.

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17.

Effects of H 2O 2-plasma processing on properties of cellular scaffolds made of В«BioplastotanВ» resorbing polyesters / E. D. Nikolaeva, D. B. Goncharov, E. I. Shishatskaya // Cellular Transplantation and Tissue Engineering. - 2011. - Vol. 6, Is. 2. - С. 65-69 . - ISSN 1815-445X
Кл.слова (ненормированные):
Adhesion -- Bioplastotan -- Cell viability -- H 2O 2-plasma processing -- Resorbing polyesters -- Scaffold
Аннотация: Produced from В«BioplastotanВ» resorbing polyesters (linear polyesters of hydroxyl derivatives alkanoic acids) scaffolds for cell culturing such as films, pressed 3-D forms and nonwoven fabric from ultrathin fibers are characterized. Two types of polymers - a homopolymer of the 3-hydroxybutyric acid and a copolymer formed by monomers of the 3-hydroxybutyric and 3-hydroxyvalerianic acids are studied. Surface properties of developed polymer scaffolds, sterilized with autoclaving and H 2O 2-plasma processing are compared. It is shown that plasma has beneficial effects resulting in decrease of the watering contact angle and increase of surface hydrophilic properties. Positive effects of H 2O 2-plasma processing of scaffold surface on culturing cell adhesion and viability compared with autoclaving sterilization is demonstrated on NIH 3T3 line fibroblast culturing.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nikolaeva, E.D.; Goncharov, D.B.; Shishatskaya, E.I.

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18.

Microbial degradation of polyhydroxyalkanoates in tropical soils / A. N. Boyandin [et al.] // Int. Biodeterior. Biodegrad. - 2013. - Vol. 83. - P77-84, DOI 10.1016/j.ibiod.2013.04.014. - Cited References: 74. - This study was financially supported by Project "Biotechnologies of novel biomaterials" (Agreement No. 11.G34.31.0013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning". . - 8. - ISSN 0964-8305

РУБ Biotechnology & Applied Microbiology + Environmental Sciences
Рубрики:
POLY-BETA-HYDROXYBUTYRATE
   CHAIN-LENGTH POLYHYDROXYALKANOATES
   POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE
   COASTAL WATERS
   PHA FILMS
   BIODEGRADATION
   BACTERIA
   IDENTIFICATION
   POLYESTERS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
Кл.слова (ненормированные):
Polyhydroxyalkanoates (PHAs) -- Biopolymers -- Biopolymer properties -- Soil biodegradation -- PHA degrading microorganisms
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. (C) 2013 Elsevier Ltd. All rights reserved.

Держатели документа:
[Boyandin, Anatoly N.
Volova, Tatiana G.
Gitelson, Iosif I.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Prudnikova, Svetlana V.
Volova, Tatiana G.] Siberian Fed Univ, Krasnoyarsk, Russia
[Karpov, Valery A.] Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Moscow, Russia
[Ivonin, Vladimir N.
Ngoc Lanh Do
Thi Hoai Nguyen
Thi My Hiep Le
Filichev, Nikolay L.
Levin, Alexander L.] Joint Russian Vietnam Trop Res & Test Ctr, Hanoi, Vietnam
[Filipenko, Maxim L.] Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Novosibirsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Prudnikova, S.V.; Karpov, V.A.; Ivonin, V.N.; Do, N.L.; Nguyen, T.H.; Le, TMH; Filichev, N.L.; Levin, A.L.; Filipenko, M.L.; Volova, T.G.; Gitelson, I.I.

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19.

Characterization of polymeric microparticles based on resorbable polyesters of oxyalkanoic acids as a platform for deposition and delivery of drugs / A. V. Goreva [et al.] // Polym. Sci. Ser. A. - 2012. - Vol. 54, Is. 2. - P94-105, DOI 10.1134/S0965545X12020022. - Cited References: 33. - This work was supported by the program for Support of Leading Scientific Schools of the Russian Federation (project no. 11.G34.31.0013.2010, Biotechnology of New Biomaterials) and the program of integrated studies of the Presidium of the Siberian Branch, Russian Academy of Sciences (project no. 93). . - 12. - ISSN 0965-545X

РУБ Polymer Science
Рубрики:
IN-VITRO RELEASE
   POLYHYDROXYBUTYRATE MICROSPHERES
   BLENDS
   RIFAMPICIN
   BIOCOMPATIBILITY
   DEGRADATION
   FORMULATION
   COMPOSITE
   CARRIERS
   MODEL
Аннотация: The effect of the preparation technique (chemical composition of a polymer, type and method of emulsion mixing, and molecular mass of a drug) on the yield, structure, and size of microparticles obtained from resorbable polyesters of microbiological origin, polyhydroxyalkanoates, is studied. It is found that the concentration of the polymer solution and the method of emulsion mixing are the most significant factors affecting the diameter of microparticles based on polyhydroxyalkanoates; the surface structure of particles depends to a higher extent on the chemical composition of the polymer. The family of microparticles from 100-200 nm to 50-70 mu m in diameter is synthesized. It is shown that the rate of drug release from microparticles in vitro into the medium is higher in the case of 3-hydroxybutyrate copolymers with 3-hydroxyvalerate than in the case of the homopolymer of 3-hydroxybutyrate. This parameter increases with the content of 3-hydroxyvalerate units in the copolymer and the porosity and mass fraction of the drug in particles with a decrease in their sizes. For in vitro systems containing a phosphate buffer, variation in the preparation parameters makes it possible to obtain microparticles with various characteristics suitable for deposition of drugs. For microparticles obtained from polyhydroxyalkanoates and having different diameters, the mathematical description of the kinetics of drug release from the polymer matrix is provided.

Держатели документа:
[Goreva, A. V.
Shishatskaya, E. I.
Volova, T. G.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
[Shishatskaya, E. I.
Volova, T. G.] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia
[Goreva, A. V.
Sinskey, A. J.] MIT, Cambridge, MA 02139 USA : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Goreva, A.V.; Shishatskaya, E.I.; Volova, T.G.; Sinskey, A.J.

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20.

A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs / T. Volova [et al.] // PLoS ONE. - 2014. - Vol. 9, Is. 2, DOI 10.1371/journal.pone.0087551 . - ISSN 1932-6203
Кл.слова (ненормированные):
3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer -- 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer -- copolymer -- gamma butyrolactone -- glucose -- hexanoic acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polystyrene -- propionic acid -- unclassified drug -- valeric acid -- animal cell -- article -- bacterial growth -- bacterium culture -- cell adhesion -- cell proliferation -- crystal structure -- culture optimization -- Cupriavidus -- Cupriavidus euthrophus -- decomposition -- elasticity -- film -- glucose utilization -- kinetics -- mechanics -- melting point -- mouse -- nonhuman -- nucleotide sequence -- physical chemistry -- polymerization -- strength -- synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Kiselev, E.; Vinogradova, O.; Nikolaeva, E.; Chistyakov, A.; Sukovatiy, A.; Shishatskaya, E.

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