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Physicochemical properties of multicomponent poly(hydroxyalkanoates) / T. G. Volova, P. V. Mironov, A. D. Vasil'ev // Biophysics. - 2007. - Vol. 52, Is. 3. - P293-297, DOI 10.1134/S0006350907030062 . - ISSN 0006-3509
Кл.слова (ненормированные):
Hydroxyhexanoate -- Hydroxyvalerate -- Poly(hydroxybutyrate) -- Bacteria (microorganisms) -- Cupriavidus necator -- Insectivora
Аннотация: The properties of new five-component poly(hydroxyalkanoates) (PHA) formed by short-and medium-chain monomers synthesized by the bacterium Wautersia eutropha B5786 were studied by X-ray diffraction, IR spectroscopy, differential thermal analysis, and viscometry. The degree of crystallinity of PHA decreased from 72 to 57% as the molar fraction of hydroxyhexanoate increased from 2.5 to 18.0 mol%. The melting temperature (T m) and decomposition temperature (T d) of the multicomponent PHA are lower than those for poly(hydroxybutyrate), whose T m and T d are 168-170 and 260-265В°C, respectively. Both parameters of the multicomponent PHA decrease to 156 and 252В°C, respectively, as the hydroxyhexanoate mole fraction is raised. The effect of hydroxyhexanoate on the physicochemical properties of the PHA is similar to that of hydroxyvalerate observed previously. В© 2007 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Siberian State Technological University, Krasnoyarsk 660049, Russian Federation
Kirenskii Institute of Physics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Mironov, P.V.; Vasil'ev, A.D.

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Physicochemical properties of two-component polyhydroxyalkanoates, 3-hydroxybutyrate-3-hydroxyvalerate copolymers / T. G. Volova [et al.] // Biophysics. - 2004. - Vol. 49, Is. 6. - P934-942 . - ISSN 0006-3509
Кл.слова (ненормированные):
Hydroxybutyrate-hydroxyvalerate copolymers -- Physicochemical properties -- Polyhydroxyalkanoates -- Structure -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: A series of two-component polyhydroxyalkanoates composed of hydroxybutyrate-hydroxyvalerate copolymers with different monomer ratio was obtained with the use of bacteria Ralstonia eutropha B5786. The properties of the polyhydroxyalkanoates in comparison with the homopolymer of hydroxybutyric acid were studied by X-ray diffraction analysis, IR spectroscopy, differential thermal analysis, and viscometry. The ratio of crystalline to amorphous phase in the copolymers tends to unity with increasing hydroxyvalerate content. This is accompanied by a decrease in the degree of crystallinity of the copolymers from 70-80 to 45-50%, the dependence is virtually linear within the range, of hydroxyvalerate mole fraction from several to 25-30 mol%. Thermal characteristics, melting temperature (Tm) and decomposition temperature (Td), of the polyhydroxyalkanoate copolymers are lower than those for polyhydroxybutyrate, whose Tm and Td are 168-170 and 260-265В°C, respectively. Both parameters decrease to 150-160 and 200-220В°C, respectively, when the hydroxyvalerate mole fraction is raised. No distinct correlation between polymer composition and molecular weight has been revealed. Copyright В© 2004 by MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian State Technological University, Krasnoyarsk, 660049, Russian Federation
Kirenskii Institute of Physics, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Plotnikov, V.F.; Shishatskaya, E.I.; Mironov, P.V.; Vasil'ev, A.D.

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Study of molecular structure of polyhydroxybutyrate-a termoplastic anddegradable biopolymer / T. G. Volova [и др.] // Biofizika. - 2000. - Vol. 45, Is. 3. - С. 445-451 . - ISSN 0006-3029
Кл.слова (ненормированные):
carbon -- hydroxybutyric acid -- polyester -- Alcaligenes -- article -- chemistry -- crystallization -- Alcaligenes -- Carbon -- Crystallization -- Hydroxybutyrates -- Polyesters
Аннотация: The molecular structure of polyhydroxybutyrate from hydrogen-oxidizing bacteria Alcaligenes eutrophus was studied by X-ray diffraction analysis. It was shown that the degree of crystallinity of various samples depends little on the conditions of their preparation and is equal to 0.62-0.76. The molecular structure of solid samples and solution of polyhydroxybutyrate in chloroform were studied by the NMR and EPR methods. The conclusion is made that the molecular structure of polyhydroxybutyrate does not depend on the features of the strain and conditions of carbon nutrition of microorganisms producing polyhydroxybutyrate. Defects induced by gamma-radiation in polyhydroxybutyrate were studied. Free radicals were isolated, and their structure was decoded.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Vasil'ev, A.D.; Zeer, E.P.; Petrakovskaia, E.A.; Falaleev, O.V.

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Surface bonding states of nano-crystalline diamond balls / J. L. Peng [et al.] // International Journal of Modern Physics B. - 2001. - Vol. 15, Is. 31. - P4071-4085, DOI 10.1142/S0217979201007865 . - ISSN 0217-9792
Кл.слова (ненормированные):
diamond -- article -- crystal structure -- electron -- energy transfer -- nanoparticle -- particulate matter -- structure analysis -- surface property -- transmission electron microscopy
Аннотация: The rough surface of nano-crystalline diamond spheres induces surface electronic states which appear as a broadened pre-peak over approx. 15 eV at the C K-edge energy threshold for carbon in the parallel electron energy loss spectrum (PEELS). This appears to be at least partially due to 1s-?* transitions, although typically the latter occupy a range of only 4 eV for the sp2 edge of highly-oriented pyrollytic graphite (HOPG). No ?* electrons appear in the conduction band inside the diamond particles, where all electrons are sp3 hybridized. PEELS data were also obtained from a chemical vapour deposited diamond film (CVDF) and gem-quality diamond for comparison with the spectra of nano-diamonds. The density of Sp2 and Sp3 states on the surface of diamond nano-crystals is calculated for simple structural models of the diamond balls, including some conjecture about surface structures. The results are used to interpret the sp2/sp3 ratios measured from the PEELS spectra recorded as scans across the particles. Surface roughness at the atomic scale was also examined using high-resolution transmission electron microscopy (HRTEM) and electron nano-diffraction patterns were used to confirm the crystal structures.

Scopus
Держатели документа:
Department of Applied Physics, RMIT University, Swanston Street, Melbourne, Vic. 3051, Australia
Electron Microscope Unit, University of Sydney, NSW 2006, Australia
Molecular Architecture Group, Kirensky Institute of Physics, Institute of Biophysics, 660036 Krasnoyarsk, Russian Federation
School of Physics, University of Melbourne, Parkville, Vic. 3010, Australia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Peng, J.L.; Bulcock, S.; Belobrov, P.I.; Bursill, L.A.

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Low-field electron emission of diamond/pyrocarbon composites / A. V. Karabutov [et al.] // Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures. - 2001. - Vol. 19: 13th International Vaccum Microelectronics Conference (14 August 2000 through 17 August 2000, Guangzhou, Is. 3. - P965-970, DOI 10.1116/1.1368669 . - ISSN 1071-1023
Кл.слова (ненормированные):
Carbon nanotubes -- Chemical bonds -- Chemical vapor deposition -- Composite materials -- Diamond films -- Electric conductivity -- Electron emission -- Electron energy levels -- Hysteresis -- Interfaces (materials) -- Raman scattering -- Semiconducting diamonds -- Semiconductor quantum wells -- Transmission electron microscopy -- X ray diffraction analysis -- X ray photoelectron spectroscopy -- Pyrocarbon composites -- Nanostructured materials
Аннотация: The properties of field electron emission for diamond/pyrocarbon nanocomposites produced from diamond particles surrounded by a pyrocarbon matrix were studied. Low-threshold emissions at fields of ?1 V/?m with no activation or hysterisis in the current versus voltage (I/V) behaviour were observed for the materials. Scanning tunneling-field emission microscopy was used to study the mechanisms of low-field electron emission from the composites, and a model based on quantum well effect at the diamond/graphite interface was proposed and discussed.

Scopus
Держатели документа:
General Physics Institute, Vavilova str. 38, Moscow 117942, Russian Federation
Central Research Institute of Materials, Paradnaya str. 8, St. Petersburg 191014, Russian Federation
Institute of Biophysics, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Karabutov, A.V.; Frolov, V.D.; Konov, V.I.; Ralchenko, V.G.; Gordeev, S.K.; Belobrov, P.I.

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Study of the molecular structure of polyhydroxybutyrate, a termoplastic and degradable biopolymer [Текст] / T. G. Volova [и др.] // Biofizika. - 2000. - Vol. 45, Is. 3. - С. 445-451. - Cited References: 16 . - 7. - ISSN 0006-3029

РУБ Biophysics
Рубрики:
POLY(BETA-HYDROXYBUTYRATE)
BIOTECHNOLOGY
Кл.слова (ненормированные):
polyhydroxybutyrate -- crystalline and amorphous phases -- molecular structure
Аннотация: The molecular structure of polyhydroxybutyrate from hydrogen-oxidizing bacteria Alcaligenes eutrophus was studied by X-ray diffraction analysis. It was shown that the degree of crystallinity of various samples depends little on the conditions of their preparation and is equal to 0.62-0.76. The molecular structure of solid samples and solution of polyhydroxybutyrate in chloroform were studied by the NMR and EPR methods. The conclusion is made that the molecular structure of polyhydroxybutyrate does not depend on the features of the strain and conditions of carbon nutrition of microorganisms producing polyhydroxybutyrate. Defects induced by gamma -radiation in polyhydroxybutyrate were studied. Free radicals were isolated, and their structure was decoded.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Russian Acad Sci, LV Kirensky Phys Inst, Siberian Branch, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Vasiliev, A.D.; Zeer, E.P.; Petrakovskaya, E.A.; Falaleev, O.V.

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All three Ca2+-binding loops of photoproteins bind calcium ions: The crystal structures of calcium-loaded apo-aequorin and apo-obelin [Text] / L. . Deng [et al.] // Protein Sci. - 2005. - Vol. 14, Is. 3. - P663-675, DOI 10.1110/ps.041142905. - Cited References: 46 . - ISSN 0961-8368

РУБ Biochemistry & Molecular Biology
Рубрики:
RAY CRYSTALLOGRAPHIC ANALYSIS
   ANGSTROM RESOLUTION
   SEQUENCE-ANALYSIS
   CA2+-REGULATED PHOTOPROTEINS
   CA2+-DISCHARGED PHOTOPROTEIN
   LUMINESCENT PROTEIN
   MODULATED PROTEINS
   ELECTRON-DENSITY
   CLONING
   CDNA
Кл.слова (ненормированные):
bioluminescence -- EF-hand -- fluorescent protein -- proton relay -- calcium-binding loops -- aequorin -- obelin -- diffraction
Аннотация: The crystal structures of calcium-loaded apo-aequorin and apo-obelin have been determined at resolutions 1.7 Angstrom and 2.2 Angstrom. respectively. A calcium ion is observed in each of the three EF-hand loops that have the canonical calcium-binding sequence, and each is coordinated in the characteristic pentagonal bipyramidal configuration. The calcium-loaded apo-proteins retain the same compact scaffold and overall fold as the unreacted photoproteins containing the bound substrate, 2-hydroperoxycoelenterazine, and also the same as the Ca2+-discharged obelin bound with the product, coelenteramide. Nevertheless, there are easily discerned shifts in both helix and loop regions, and the shifts are not the same between the two proteins. It is suggested that these subtle shifts are the basis of the ability of these photoproteins to sense Ca2+ concentration transients and to produce their bioluminescence response on the millisecond timescale. A mechanism of intrastructural transmission of the calcium signal is proposed.

Держатели документа:
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Univ Georgia, Dept Chem, Athens, GA 30602 USA
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Deng, L...; Vysotski, E.S.; Markova, S.V.; Liu, Z.J.; Lee, J...; Rose, J...; Wang, B.C.

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Preparation and X-ray crystallographic analysis of the Ca2+-discharged photoprotein obelin [Text] / L. . Deng [et al.] // Acta Crystallogr. Sect. D-Biol. Crystallogr. - 2004. - Vol. 60. - P512-514, DOI 10.1107/S090744490302852X. - Cited References: 18 . - ISSN 0907-4449

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Biophysics + Crystallography
Рубрики:
VIOLET BIOLUMINESCENCE
   ANGSTROM RESOLUTION
   W92F OBELIN
   AEQUORIN
   SEQUENCE
   PROTEIN
   CLONING
   CDNA
Аннотация: Ca2+-regulated photoproteins belong to the EF-hand Ca2+-binding protein family. The addition of calcium ions initiates bright blue bioluminescence of the photoproteins, a result of the oxidative breakdown of coelenterazine peroxide to coelenteramide. Crystals of the Ca2+-discharged W92F mutant of obelin from Obelia longissima have been grown, representing the first crystallization of a photoprotein after the Ca2+-triggered bioluminescence. A green fluorescence observed from the crystals clearly demonstrates that coelenteramide, the bioluminescence product of coelenterazine peroxide, is bound within the protein. The diffraction pattern exhibits tetragonal Laue symmetry. Systematic absences indicate that the space group is either P4(3)2(1)2 or P4(1)2(1)2. The unit-cell parameters are a=b=53.4, c=144.0 Angstrom. The crystals diffract to 1.9 Angstrom resolution.

Держатели документа:
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Univ Georgia, Dept Chem, Athens, GA 30602 USA
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Deng, L...; Markova, S.V.; Vysotski, E.S.; Liu, Z.J.; Lee, J...; Rose, J...; Wang, B.C.

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Structure of the Ca2+-regulated photoprotein obelin at 1.7 angstrom resolution determined directly from its sulfur substructure [Text] / Z. J. Liu [et al.] // Protein Sci. - 2000. - Vol. 9, Is. 11. - P2085-2093. - Cited References: 41 . - ISSN 0961-8368

РУБ Biochemistry & Molecular Biology
Рубрики:
CALCIUM-MODULATED PROTEINS
   AMINO-ACID SEQUENCE
   CA-2+-BINDING PHOTOPROTEIN
   CA2+-BINDING PHOTOPROTEIN
   MACROMOLECULAR STRUCTURES
   3-DIMENSIONAL STRUCTURE
   ANOMALOUS SCATTERING
   CRYSTAL-STRUCTURES
   DIFFRACTION DATA
   BINDING SITE
Кл.слова (ненормированные):
bioluminescence -- crystallography -- obelin -- photoprotein -- single wavelength anomalous scattering -- solvent flattening -- sulfur phasing
Аннотация: The crystal structure of the photoprotein obelin (22.2 kDa) from Obelia longissima has been determined and refined to 1.7 Angstrom resolution. Contrary to the prediction of a peroxide, the noncovalently bound substrate, coelenterazine, has only a single oxygen atom bound at the C2-position. The protein-coelenterazine 2-oxy complex observed in the crystals is photo-active because, in the presence of calcium ion, bioluminescence emission within the crystal is observed. This structure represents only the second de novo protein structure determined using the anomalous scattering signal of the sulfur substructure in the crystal. The method used here is theoretically different from that used for crambin in 1981 (4.72 kDa) and represents a significant advancement in protein crystal structure determination.

Держатели документа:
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Liu, Z.J.; Vysotski, E.S.; Chen, C.J.; Rose, J.P.; Lee, J...; Wang, B.C.

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10.

Preparation and preliminary study of crystals of the recombinant calcium-regulated photoprotein obelin from the bioluminescent hydroid Obelia longissima [Text] / E. S. Vysotski [et al.] // Acta Crystallogr. Sect. D-Biol. Crystallogr. - 1999. - Vol. 55. - P1965-1966, DOI 10.1107/S0907444999011828. - Cited References: 23 . - ISSN 0907-4449

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Biophysics + Crystallography
Рубрики:
AEQUORIN
LUCIFERASE
LIGHT
Аннотация: Crystals of recombinant obelin, the Ca2+-regulated photoprotein from the marine hydroid Obelia longissima, have been grown from sodium citrate solutions. Crystals grow as hexagonal light-yellow rods (0.1 x 0.1 x 1.0 mm) which diffract to beyond 1.8 Angstrom with synchrotron radiation of 1.0 Angstrom wavelength. The crystals have a primitive hexagonal lattice with unit-cell parameters a = 81.55, c = 86.95 Angstrom. The asymmetric unit contains two molecules. This represents the successful preparation of single crystals of a photoprotein obelin which have promising diffraction properties.

Держатели документа:
Russian Acad Sci, Photobiol Lab, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vysotski, E.S.; Liu, Z.J.; Rose, J...; Wang, B.C.; Lee, J...

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11.

Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state [Text] / Z. J. Liu [et al.] // Proc. Natl. Acad. Sci. U. S. A. - 2006. - Vol. 103, Is. 8. - P2570-2575, DOI 10.1073/pnas.0511142103. - Cited References: 51 . - ISSN 0027-8424

РУБ Multidisciplinary Sciences
Рубрики:
X-RAY-DIFFRACTION
   ANGSTROM RESOLUTION
   CA2+-REGULATED PHOTOPROTEINS
   AEQUORIN BIOLUMINESCENCE
   VIOLET BIOLUMINESCENCE
   W92F OBELIN
   PROTEIN
   LUCIFERASE
   LIGHT
   PROGRAM
Кл.слова (ненормированные):
coelenterazine -- photoprotein -- EF hand -- luciferase -- aequorin
Аннотация: The crystal structure at 1.93-angstrom resolution is determined for the Ca2+-discharged obelin containing three bound calcium ions as well as the product of the bioluminescence reaction, coelenteramide. This finding extends the series of available spatial structures of the ligand-dependent conformations of the protein to four, the obelin itself, and those after the bioluminescence reaction with or without bound Ca2+ and/or coelenteramide. Among these structures, global conformational changes are small, typical of the class of "calcium signal modulators" within the EF-hand protein superfamily. Nevertheless, in the active site there are significant repositions of two residues. The His-175 imidazole ring flips becoming almost perpendicular to the original orientation corroborating the crucial importance of this residue for triggering bioluminescence. Tyr-138 hydrogen bonded to the coelenterazine N1-atom in unreacted obelin is moved away from the binding cavity after reaction. However, this Tyr is displaced by a water molecule from within the cavity, which now forms a hydrogen bond to the same atom, the amide N of coelenteramide. From this observation, a reaction scheme is proposed that would result in the neutral coelenteramide as the primary excited state product in photoprotein bioluminescence. From such a higher energy state it is now energetically feasible to account for the shorter wavelength bioluminescence spectra obtained from some photoprotein mutants or to populate the lower energy state of the phenolate anion to yield the blue bioluminescence ordinarily observed from native photoproteins.

Держатели документа:
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Liu, Z.J.; Stepanyuk, G.A.; Vysotski, E.S.; Lee, J...; Markova, S.V.; Malikova, N.P.; Wang, B.C.

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12.

Surface bonding states of nano-crystalline diamond balls [Text] / J. L. Peng [et al.] // Int. J. Mod. Phys. B. - 2001. - Vol. 15, Is. 31. - P. 4071-4085, DOI 10.1142/S0217979201007865. - Cited References: 20 . - ISSN 0217-9792

РУБ Physics, Applied + Physics, Condensed Matter + Physics, Mathematical
Рубрики:
PLASMON RESPONSE
   POWDER
   SPECTROSCOPY
   MICROSCOPY
   SILICON
   SI(111)
Аннотация: The rough surface of nano-crystalline diamond spheres induces surface electronic states which appear as a broadened pre-peak over approx. 15 eV at the C K-edge energy threshold for carbon in the parallel electron energy loss spectrum (PEELS). This appears to be at least partially due to 1s-pi* transitions, although typically the latter occupy a range of only 4 eV for the sp(2) edge of highly-oriented pyrollytic graphite (HOPG). No pi* electrons appear in the conduction band inside the diamond particles, where all electrons are sp(3) hybridized. PEELS data were also obtained from a chemical vapour deposited diamond film (CVDF) and gem-quality diamond for comparison with the spectra of nano-diamonds. The density of sp(2) and sp(3) states on the surface of diamond nano-crystals is calculated for simple structural models of the diamond balls, including some conjecture about surface structures. The results are used to interpret the sp(2)/sp(3) ratios measured from the PEELS spectra recorded as scans across the particles. Surface roughness at the atomic scale was also examined using high-resolution transmission electron microscopy (HRTEM) and electron nano-diffraction patterns were used to confirm the crystal structures.

WOS
Держатели документа:
RMIT Univ, Dept Appl Phys, Melbourne, Vic 3051, Australia
Univ Sydney, Electron Microscope Unit, Sydney, NSW 2006, Australia
Russian Acad Sci, Siberian Branch, LV Kirensky Phys Inst, Mol Architecture Grp, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
Univ Melbourne, Sch Phys, Parkville, Vic 3052, Australia
ИФ СО РАН
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Peng, J.L.; Bulcock, S...; Belobrov, P.I.; Bursill, L.A.

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13.

Biosynthesis of tetrahydrofolate in plants: Crystal structure of 7,8-dihydroneopterin aldolase from Arabidopsis thaliana reveals a novel adolase class [Text] / S. . Bauer [et al.] // J. Mol. Biol. - 2004. - Vol. 339, Is. 4. - P. 967-979, DOI 10.1016/j.jmb.2004.04.034. - Cited References: 66 . - ISSN 0022-2836

РУБ Biochemistry & Molecular Biology
Рубрики:
GTP CYCLOHYDROLASE-I
   GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE
   6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE
   ESCHERICHIA-COLI
   DIHYDRONEOPTERIN ALDOLASE
   FOLIC-ACID
   ENZYMATIC SYNTHESIS
   DIHYDROPTEROATE SYNTHASE
   REACTION-MECHANISM
   3-DIMENSIONAL STRUCTURE
Кл.слова (ненормированные):
tetrahydrofolate biosynthesis -- aldolase classes -- retroaldol reaction -- purin binding -- Schiff base
Аннотация: Dihydroneopterin aldolase (DHNA) catalyses a retroaldol reaction yielding 6-hydroxymethyl-7,8-dihydropterin, a biosynthetic precursor of the vitamin, tetrahydrofolate. The enzyme is a potential target for antimicrobial and anti-parasite chemotherapy. A gene specifying a dihydroneopterin aldolase from Arabidopsis thaliana was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity and crystallised using polyethylenglycol as the precipitating agent. The crystal structure was solved by X-ray diffraction analysis at 2.2 Angstrom resolution. The enzyme forms a D-4-symmetric homo-octamer. Each polypeptide chain is folded into a single domain comprising an antiparallel four-stranded beta-sheet and two long alpha-helices. Four monomers are arranged in a tetrameric ring, and two of these rings form a hollow cylinder. Well defined purine derivatives are found at all eight topologically equivalent active sites. The subunit fold of the enzyme is related to substructures of dihydroneopterin triphosphate epimerase, GTP cyclohydrolase I, and pyruvoyltetrahydropterin synthase, which are all involved in the biosynthesis of pteridine type cofactors, and to urate oxidase, although some members of that superfamily have no detectable sequence similarity Due to structural and mechanistical differences of DHNA in comparison with class I and class II aldolases, a new aldolase class is proposed. (C) 2004 Elsevier Ltd. All rights reserved.

WOS
Держатели документа:
Max Planck Inst Biochem, Abt Strukturforsch, D-82152 Martinsried, Germany
Tech Univ Munich, Lehrstuhl Organ Chem & Biochem, D-85747 Garching, Germany
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bauer, S...; Schott, A.K.; Illarionova, V...; Bacher, A...; Huber, R...; Fischer, M...

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14.

Bio-hybridization of nanobactericides with cellulose films for effective treatment against members of ESKAPE multi-drug-resistant pathogens / S. Baker [et al.] // Appl. Nanosci. - 2018. - Vol. 8, Is. 5. - P1101-1110, DOI 10.1007/s13204-018-0717-9. - Cited References:51. - Authors are thankful for Ministry of Education and Science of the Russian Federation for providing funding under the scheme of 5-100: Russian Academic Excellence Project. Authors are grateful for facilities provided by Siberian Federal University to carry out the present study. . - ISSN 2190-5509. - ISSN 2190-5517

РУБ Nanoscience & Nanotechnology
Рубрики:
SILVER NANOPARTICLES
BACTERIAL CELLULOSE
ANTIBIOTIC-RESISTANCE
Кл.слова (ненормированные):
ESKAPE -- Bio-hybridization -- Silver nanobactericides -- Phytogenic -- Bactericidal activity
Аннотация: The rapid expansion of drug-resistant pathogens has created huge global impact and development of novel antimicrobial leads is one of the top priority studies in the current scenario. The present study aims to develop bio-hybridized nanocellulose films which comprise of phytogenic silver nanobactericides. The nanobactericides were synthesized by treating 1 mM silver nitrate with aqueous extract of Chamerion angustifolium which reduced the metal salt to produce polydispersed nanobactericides which were tested against the members of ESKAPE drug-resistant communities. The synthesized silver nanobactericides were subjected to characterization with UV-visible spectra which displayed maximum absorbance at 408 nm. The bio-molecular interaction of phyto-constituents to mediate synthesis and stabilization of nanobactericides was studied with Fourier-transform infrared spectroscopy (FTIR) which depicted functional groups associated with nanobactericides. The crystalline nature was studied with X-ray diffraction (XRD) which showed Bragg's intensities at 2 theta angle which denoted (111), (200), (220), and (311) planes. The morphological characteristics of silver nanobactericides were defined with transmission electron Microscopy (TEM) image which displayed polydispersity of silver nanobactericides with size ranging from 2 to 40 nm. The synthesized nanobactericides showed a significant activity against MRSA strain with 21 mm zone of inhibition. The minimal inhibitory concentration of silver nanobactericides to inhibit the growth of test pathogens was also determined which ranged between 0.625 and 1.25 mu g/ml. The silver nanobactericides were bio-hybridized onto nanocellulose films produced by Komagataeibacter xylinus B-12068 culture strain. The films were dried to determine the mechanical properties which showed increased in Young's modulus and tensile strength in comparison with control bacterial cellulose films. Overall, the results obtained in the present investigation are promising enough to report bactericidal activity of bio-hybridized nanobactericidal films against ESKAPE. These communities are reported to cause severe threats to all forms of lives irrespective to their habitats which can lead to huge economical crisis.

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Держатели документа:
Siberian Fed Univ, Lab Biotechnol New Mat, Svobodnyy Pr 79, Krasnoyarsk 660041, Russia.
SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Inst Biophys, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Krasnoyarsk, Russia.
Krasnoyasrk State Med Univ, Dept Microbiol, Krasnoyarsk Partizana Zheleznyaka St 1, Krasnoyarsk 660022, Russia.
SB RAS, Fed Res Ctr KSC, Kirensky Inst Phys, Akademgorodok 50,Bld 38, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Sch Petr & Nat Gas Engn, Krasnoyarsk, Russia.

Доп.точки доступа:
Baker, Syed; Volova, Tatiana; Prudnikova, Svetlana, V; Shumilova, Anna A.; Perianova, Olga, V; Zharkov, Sergey M.; Kuzmin, And Rey; Olga, Kondratenka; Bogdan, Kiryukhin; Shidlovskiy, Ivan P.; Potkina, Zoya K.; Khohlova, Olga Y.; Lobova, Tatiana, I; Ministry of Education and Science of the Russian Federation under the scheme of 5-100: Russian Academic Excellence Project

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15.

Properties of Bacterial Cellulose Composites with Silver Nanoparticles / I. P. Shidlovskiy [et al.] // Biophysics. - 2018. - Vol. 63, Is. 4. - P519-525, DOI 10.1134/S0006350918040188 . - ISSN 0006-3509
Кл.слова (ненормированные):
:bacterial cellulose -- antibacterial activity -- composites -- hydrothermal synthesis -- silver nanoparticles
Аннотация: Abstract: Composites of bacterial cellulose, which were synthesized in a culture of the strain of acetic acid bacteria Komagataeibacter xylinus VKPM B-12068, with silver nanoparticles were produced hydrothermally by varying the concentrations of AgNO3 in the medium. The presence of silver in the composites was confirmed by elemental analysis. An increase in the number of silver nanoparticles in the composite from 1.08 to 9.1 wt % (from 0.044 to 0.370 mg/cm2) was shown under increasing AgNO3 concentration in the medium from 0.0001 to 0.01 M. The structure, properties of the surface, and the physicochemical properties of the composites depending on the silver content were investigated using scanning electron microscopy, differential scanning calorimetry, X-ray diffraction, and a water contact-angle measurement system. Using the disk-diffusion method, it was shown that the resulting composites have a pronounced antibacterial activity against pathogenic microflora E. coli, Ps. eruginosa, and St. aureus. © 2018, Pleiades Publishing, Inc.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Shidlovskiy, I. P.; Shumilova, A. A.; Shishatskaya, E. I.; Volova, T. G.

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16.

Bioluminescence chemistry of fireworm Odontosyllis / A. A. Kotlobay [et al.] // Proc. Natl. Acad. Sci. U. S. A. - 2019. - Vol. 116, Is. 38. - P18911-18916, DOI 10.1073/pnas.1902095116. - Cited References:16. - We thank the late Dr. Shoji Inoue and Dr. Hisae Kakoi (Meijo University) for providing Odontosyllis materials, Sergey Shakhov for photography, and Drs. Mikhail Baranov and Andrey Mikhaylov for discussions. Some experiments were carried out using equipment provided by the Institute of Bioorganic Chemistry of the Russian Academy of Sciences.ore Facility. Some experiments were supported by Planta LLC. Structural and mechanistic studies were supported by Russian Science Foundation Grant 18-74-10102. Isolation, purification, and biochemical studies were supported by Russian Science Foundation Grant 16-14-00052p. B.R.B. acknowledges support from the Air Force Office of Scientific Research (FA9550-18-1-0017). . - ISSN 0027-8424

РУБ Multidisciplinary Sciences
Рубрики:
MECHANISM
DECARBOXYLATION
OXIDATION
Кл.слова (ненормированные):
bioluminescence -- Odontosyllis luciferin -- oxyluciferin -- heterocycles
Аннотация: Marine polychaetes Odontosyllis undecimdonta, commonly known as fireworms, emit bright blue-green bioluminescence. Until the recent identification of the Odontosyllis luciferase enzyme, little progress had been made toward characterizing the key components of this bioluminescence system. Here we present the biomolecular mechanisms of enzymatic (leading to light emission) and nonenzymatic (dark) oxidation pathways of newly described O. undecimdonta luciferin. Spectral studies, including 1D and 2D NMR spectroscopy, mass spectrometry, and X-ray diffraction, of isolated substances allowed us to characterize the luciferin as an unusual tricyclic sulfur-containing heterocycle. Odontosyllis luciferin does not share structural similarity with any other known luciferins. The structures of the Odontosyllis bioluminescent system's low molecular weight components have enabled us to propose chemical transformation pathways for the enzymatic and nonspecific oxidation of luciferin.

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Держатели документа:
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia.
Moscow Inst Phys & Technol, Dolgoprudnyi 141701, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
Pirogov Russian Natl Res Med Univ, Moscow 117997, Russia.
Russian Acad Sci, AN Nesmeyanov Inst Organoelement Cpds, Moscow 119991, Russia.
Natl Res Ctr, Kurchatov Inst, Moscow 123182, Russia.
St Petersburg Natl Res Acad Univ, Russian Acad Sci, St Petersburg 194021, Russia.
Connecticut Coll, New London, CT 06320 USA.
European Mol Biol Lab Hamburg, D-22603 Hamburg, Germany.
Chubu Univ, Dept Environm Biol, Kasugai, Aichi 4878501, Japan.

Доп.точки доступа:
Kotlobay, Alexey A.; Dubinnyi, Maxim A.; Purtov, Konstantin V.; Guglya, Elena B.; Rodionova, Natalja S.; Petushkov, Valentin N.; Bolt, Yaroslav V.; Kublitski, Vadim S.; Kaskova, Zinaida M.; Ziganshin, Rustam H.; Nelyubina, Yulia V.; Dorovatovskii, Pavel V.; Eliseev, Igor E.; Branchini, Bruce R.; Bourenkov, Gleb; Ivanov, Igor A.; Oba, Yuichi; Yampolsky, Ilia V.; Tsarkova, Aleksandra S.; Kaskova, Zinaida; Russian Science FoundationRussian Science Foundation (RSF) [18-74-10102, 16-14-00052p]; Air Force Office of Scientific ResearchUnited States Department of DefenseAir Force Office of Scientific Research (AFOSR) [FA9550-18-1-0017]

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