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A general one-dimensional vertical ecosystem model of Lake Shira (Russia, Khakasia): Description, parametrization and analysis / I. G. Prokopkin [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P585-618, DOI 10.1007/s10452-010-9326-8 . - ISSN 1386-2588
Кл.слова (ненормированные):
Meromictic brackish lake -- One-dimensional vertical ecological model -- Sensitivity analysis -- alga -- biomass -- brackish water -- diffusion -- ecosystem modeling -- meromictic lake -- microbial community -- model test -- nutrient -- one-dimensional modeling -- parameterization -- phytoplankton -- seasonality -- sensitivity analysis -- solar radiation -- vertical profile -- water chemistry -- water temperature -- zooplankton -- Khakassia -- Lake Shira -- Russian Federation -- algae
Аннотация: A one-dimensional ecological model of the meromictic brackish Lake Shira (Russia, Khakasia) was developed. The model incorporates state-of-the-art knowledge about the functioning of the lake ecosystem using the most recent field observations and ideas from PCLake, a general ecosystem model of shallow freshwater lakes. The model of Lake Shira presented here takes into account the vertical dynamics of biomasses of the main species of algae, zooplankton and microbial community, as well as the dynamics of oxygen, detritus, nutrients and hydrogen sulphide from spring to autumn. Solar radiation, temperature and diffusion are modelled using real meteorological data. The parameters of the model were calibrated to the field data, after applying different methods of sensitivity analysis to the model. The resulting patterns of phytoplankton and nutrients dynamics show a good qualitative and quantitative agreement with the field observations during the whole summer season. Results are less satisfactory with respect to the vertical distribution of zooplankton biomass. We hypothesize that this is due to the fact that the current model does not take the sex and age structure of zooplankton into account. The dynamics of oxygen, hydrogen sulphide and the modelled positions of the chemocline and thermocline are again in good agreement with field data. This resemblance confirms the validity of the approach we took in the model regarding the main physical, chemical and ecological processes. This general model opens the way for checking various hypotheses on the functioning of the Lake Shira ecosystem in future investigations and for analysing options for management of this economically important lake. В© 2010 Springer Science+Business Media B.V.

Scopus
Держатели документа:
Institute of Biophysics, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Netherlands Institute of Ecology, Centre for Limnology, Rijksstraatweg 6, 3631 AC Nieuwersluis, Netherlands
Netherlands Environmental Assessment Agency (PBL), Postbus 303, 3720 AH Bilthoven, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Prokopkin, I.G.; Mooij, W.M.; Janse, J.H.; Degermendzhy, A.G.

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Understanding the mechanisms of blooming of phytoplankton in Lake Shira, a saline lake in Siberia (the Republic of Khakasia) / A. G. Degermendzhy, R. D. Gulati // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P331-340 . - ISSN 1386-2588
Кл.слова (ненормированные):
Carbon budget -- Cyanobacteria -- Heterotrophic bacteria -- Hydrogen sulphide -- Mathematical models of stratification -- Meromictic lakes -- Microbial loop -- Stratification -- Trophic scheme -- algal bloom -- ecosystem modeling -- limiting factor -- nutrient availability -- phytoplankton -- saline lake -- trophic interaction -- Russian Federation -- algae -- Bacteria (microorganisms) -- Cyanobacteria -- Lyngbya -- Lyngbya contorta
Аннотация: The paper summarises the results of a three-year research study (European Union Grant: INTAS 97-0519) aimed at investigating the planktonic populations and trophic organization of the Lake Shira ecosystem - a saline lake in Khakasia, Siberia. The lake exhibits a stable summer-autumn stratification of the chemical-biological components. The mechanisms responsible for the 'blooming' of phytoplankton in the deeper layers were investigated in greater detail, using data from both field and laboratory experiments. The spectra of nutrition were examined to estimate the relationships between the specific growth rates of the hydrobionts and the influence of the limiting factors: light, nutrients. The observed heterotrophic capability of a metalimnetic phytoplankton population might help explain the development in the deeper waters of Lyngbya contorta. The scheme of trophic interactions was put up, based on the assessment of the carbon pools and carbon flows in the pelagic zone of the lake. A mathematical model of the vertical structure of the lake's plankton populations was constructed, using the ecosystem description and data of vertical turbulent diffusion. The role of light and nutrient limitations and grazing mortality in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with the field observations, e.g. for the different patterns of the peaks, and for the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. Based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been theoretically discriminated for the first time. It has also been ascertained that irrespective of the depth the allochthonous substances limiting bacterial growth, the bacteria are uniformly distributed over depth and can serve as an indicator of the presence of limitation (the effect of autostabilisation in space). Of indisputable interest to limnology are the specific methods developed for understanding the functioning of Lake Shira ecosystem. These include the autostabilisation of the limiting factors, the on-the-spot fluorescent method of determining the three classes of microalgae, the algal mixotrophy and the planktonic population interactions and feedbacks, and development of a more sensitive, bioluminescent method for mapping the nonhomogeneities. Owing to a balanced combination of classical approaches (field observations, in situ data on production-decomposition) and the more recent ones (satellite monitoring, biophysical methods of estimating interactions of populations, mathematical models based on the field and experimental data), many of the structural-function relationships in the ecosystem can now be explained, and the models can provide 'mutual control and mutual agreement' between the data collected using different approaches.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Gulati, R.D.

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Formation of the vertical heterogeneity in the Lake Shira ecosystem: The biological mechanisms and mathematical model / A. G. Degermendzhy [et al.] // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P271-297, DOI 10.1023/A:1015621508971 . - ISSN 1386-2588
Кл.слова (ненормированные):
Hydrogen sulphide -- Phytoplankton -- Stratification control -- Sulphate-reducing bacteria -- Sulphur cycle -- Vertical model -- biological production -- community structure -- ecosystem modeling -- nutrient cycling -- plankton -- saline lake -- seasonal variation -- vertical distribution -- Russian Federation -- algae -- Arctodiaptomus -- Bacteria (microorganisms) -- Calanoida -- Chlorophyta -- Copepoda -- Crustacea -- Cyanobacteria -- Dictyosphaerium -- Lyngbya -- Lyngbya contorta
Аннотация: Data on the seasonal changes in vertical heterogeneity of the physical-chemical and biological parameters of the thermally stratified Shira Lake ecosystem (Khakasia, Siberia) in 1996-2000 have been analyzed. The interaction mechanisms involving: (1) The plankton populations in aerobic and anaerobic zones, involving the cycling of carbon and sulphur, (2) the primary production limitation (by light and phosphorus) and inhibition (by light), and (3) the kinetic characteristics of plankton populations have been elucidated. A mathematical model of the vertical structure of the lake's plankton populations, based on the ecosystem description and on vertical turbulent diffusion of the matter, has been constructed. The green alga Dictyosphaerium tetrachotomum (Chlorophyta) and the cyanobacterium Lyngbya contorta (Cyanophyta), which dominated the phytoplankton biomass, were taken as oxygen producers. Arctodiaptomus salinus (a calanoid copepod) has been assumed as the main grazer in Shira Lake as it dominated the zooplankton biomass. Four groups of microorganisms involved in the sulphur cycle formation have been distinguished: sulphur, sulphur purple, sulphur green and SRB. H2S is oxidized to sulphate (only the green sulphur bacteria oxidize it to sulphur), and sulphate is reduced to H2S, forming neither sulphur nor its water-soluble compounds. The role of grazing, light and nutrient limitation, in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. When the model takes into account both light limitation and nutrient limitation of algal growth by P and consumption of algae by crustaceans: (a) in the scenario where the P is formed only by the cycling and decomposition of autochthonous organic matter, both the green algae and cyanobacteria are eliminated; (b) in the scenario involving an additional P flux in the deep water layers the peak of the cyanobacteria is at a depth of 10 m, and its amplitude is close to the one observed in the lake. The position of the peak remains stable owing to the 'double' limitation mechanism: light 'from above' and P 'from below'. Another mechanism responsible for the deep position of the peak of cyanobacteria was analyzed mathematically based on the model involving the experimentally proven assumption of the growth inhibition by light in the epilimnion and the light limitation in the hypolimnion. The main result is: the peak is positioned stable at its depth and does not change with time. The analytical and numerical calculations made for this positioning mechanism yielded the formulae relating the depth of the maximum of algal biomass, the 'width' of the peak base and the peak amplitude and a number of parameters (algae elimination, turbulent diffusion coefficient, sedimentation rate, light extinction coefficient and light intensity). The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with field observations, e.g. for the different patterns for the peaks, and the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. For the first time, theoretically, based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been discriminated. The theoretical limit for the depth up to which the hydrogen-sulphide zone can ascend under the impact of allochthonous organic loading, has been determined.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Belolipetsky, V.M.; Zotina, T.A.; Gulati, R.D.

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Electrosynthesis of hydrogen peroxide from oxygen in a gas-diffusion electrode in solutions of mineralized exometabolites / G. A. Kolyagin [et al.] // Russian Journal of Electrochemistry. - 2013. - Vol. 49, Is. 10. - P1004-1007, DOI 10.1134/S1023193513100091 . - ISSN 1023-1935
Кл.слова (ненормированные):
electroreduction of oxygen -- electrosynthesis of hydrogen peroxide -- gas diffusion electrode -- life-support system -- mineralization of biowaste -- processing of exometabolites
Аннотация: The electrosynthesis of H2O2 in aqueous solutions (pH 1-9) of mineralized liquid and solid bio-wastes (exometabolites) for their processing in closed life-support systems was studied. It was shown that H 2O2 can be obtained in these solutions by electrochemical reduction of oxygen in carbon black gas diffuse electrodes to concentrations of over 2 M with current efficiency 80%. The composition of the solution was found to affect the accumulation of H2O2 during the synthesis. The solutions can be concentrated further to 19 M H2O2. The results showed that the electrolytic method is promising for preparing H2O2 for closed life-support systems. В© 2013 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kolyagin, G.A.; Kornienko, V.L.; Kudenko, Y.A.; Tikhomirov, A.A.; Trifonov, S.V.

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Numerical modeling of the hydrophysical influence effects on the phytoplankton distribution / V. Kartushinsky Alexei // Mathematical Biology and Bioinformatics. - 2012. - Vol. 7, Is. 1. - С. 112-124 . - ISSN 1994-6538
Кл.слова (ненормированные):
Aquatic ecosystem -- Biogenic element -- Chlorophyll -- Hydrophysical processes -- Inhomogeneity -- Numerical model -- Phytoplankton -- Rainfall -- Turbulent diffusion -- Upwelling -- Variability
Аннотация: The paper describes simulating results of the phytoplankton vertical distribution in the aquatic ecosystems based on one-dimensional numerical model. The model is used to calculate the dynamics of the temperature, salt, limiting nutrient and phytoplankton biomass. The work shows some temporal variability of phytoplankton distribution in the surface layer of salt and freshwater lake ecosystems under the impact of hydrophysical processes. These results can be used for the satellite date interpretation.

Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kartushinsky Alexei, V.

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The investigation on the dynamics of frontal zones in the ocean based on the numerical modelling, using the AVHRR satellite data [Text] / A. V. Kartushinsky ; ed. P Schlussel [et al.] // CLIMATE CHANGE PROCESSES IN THE STRATOSPHERE, EARTH-ATMOSPHERE-OCEAN SYSTEMS, AND OCEANOGRAPHIC PROCESSES FROM SATELLITE DATA. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2004. - Vol. 33: 2nd World Space Congress/34th COSPAR Scientific Assembly (OCT 10-19, 2002, HOUSTON, TX), Is. 7. - P1173-1178, DOI 10.1016/S0273-1177(03)00370-3. - Cited References: 19 . - 6. - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Environmental Sciences + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences + Remote Sensing
Рубрики:
VARIABILITY
Кл.слова (ненормированные):
frontal temperature zones -- model -- numerical experiments -- current velocity -- turbulent diffusion -- heat flow
Аннотация: The main purpose of the work is to investigate the frontal temperature zones using the mathematical model of the oceanic temperature field. The forecast of the frontal temperature zone variability is based on satellite data. The model calculates the temperature under different starting conditions and allows for the mean monthly intensity of solar radiation, components of current velocities, and turbulent diffusion. The input data are the mean monthly current velocities and the radiation heat flows. The model is used to calculate the dynamics of the frontal temperature zones in separate parts of the ocean. Based on the results of numerical experiments presented in the paper we estimated the spatial and temporal ranges of the frontal zone variation affected by the advection of currents, horizontal turbulent heat exchange, and the radiation heat flow in separate parts of the ocean. As examples we consider the basic frontal zones in the Atlantic and the Pacific Oceans. The work also shows possible applications of parametrization of spatial horizontal components of the frontal zones functionally related to the processes of heat advection and diffusion in the absence of exact information about the magnitudes of the current velocities and turbulent mixing. (C) 2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

Держатели документа:
Krasnoyarsk State Univ, Inst Biophys, Russian Acad Sci, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kartushinsky, A.V.; Schlussel, P \ed.\; Stuhlmann, R \ed.\; Campbell, JW \ed.\

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Green-Fluorescent Protein from the Bioluminescent Jellyfish Clytia gregaria Is an Obligate Dimer and Does Not Form a Stable Complex with the Ca2+-Discharged Photoprotein Clytin [Text] / N. P. Malikova [et al.] // Biochemistry. - 2011. - Vol. 50, Is. 20. - P4232-4241, DOI 10.1021/bi101671p. - Cited References: 50. - This work was supported by NATO Collaborative Linkage Grant 979229, Grants SB RAS No. 2 and RFBR 08-04-92209, 09-04-12022, and 09-04-00172, the MCB program of the Russian Academy of Sciences, and Bayer AG. . - ISSN 0006-2960

РУБ Biochemistry & Molecular Biology
Рубрики:
VIBRIO-FISCHERI Y1
   ENERGY-TRANSFER
   CORRELATION SPECTROSCOPY
   BACTERIAL LUCIFERASE
   REFRACTIVE-INDEX
   PHOTOBACTERIUM-LEIOGNATHI
   POLARIZED FLUORESCENCE
   EXCITATION TRANSFER
   RECOMBINANT OBELIN
   LUMAZINE PROTEIN
Аннотация: Green-fluorescent protein (GFP) is the origin of the green bioluminescence color exhibited by several marine hydrozoans and anthozoans. The mechanism is believed to be Forster resonance energy transfer (FRET) within a luciferase GFP or photoprotein-GFP complex. As the effect is found in vitro at micromolar concentrations, for FRET to occur this complex must have an affinity in the micromolar range. We present here a fluorescence dynamics investigation of the recombinant bioluminescence proteins from the jellyfish Clytia gregaria, the photoprotein clytin in its Ca2+-discharged form that is highly fluorescent (lambda(max) = 506 nm) and its GFP (cgreGFP; lambda(max) = 500 nm). Ca2+-discharged clytin shows a predominant fluorescence lifetime of 5.7 ns, which is assigned to the final emitting state of the bioluminescence reaction product, coelenteramide anion, and a fluorescence anisotropy decay or rotational correlation time of 12 ns (20 degrees C), consistent with tight binding and rotation with the whole protein. A 34 ns correlation time combined with a translational diffusion constant and molecular brightness from fluorescence fluctuation spectroscopy all confirm that cgreGFP is an obligate dimer down to nanomolar concentrations. Within the dimer, the two chromophores have a coupled excited-state transition yielding fluorescence depolarization via FRET with a transfer correlation time of 0.5 ns. The 34 ns time of cgreGFP showed no change upon addition of a 1000-fold excess of Ca2+-discharged clytin, indicating no stable complexation below 0.2 mM. It is proposed that any bioluminescence FRET complex with micromolar affinity must be one formed transiently by the cgreGFP dimer with a short-lived (millisecond) intermediate in the clytin reaction pathway.

Держатели документа:
[Lee, John] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
[Malikova, Natalia P.
Vysotski, Eugene S.] Russian Acad Sci, Siberian Branch, Photobiol Lab, Inst Biophys, Krasnoyarsk 660036, Russia
[Visser, Nina V.
van Hoek, Arie] Wageningen Univ, Biophys Lab, NL-6703 HA Wageningen, Netherlands
[Visser, Antonie J. W. G.] Wageningen Univ, Biochem Lab, NL-6703 HA Wageningen, Netherlands
[Visser, Nina V.
van Hoek, Arie
Visser, Antonie J. W. G.] Wageningen Univ, Microspect Ctr, NL-6703 HA Wageningen, Netherlands
[Skakun, Victor V.] Belarusian State Univ, Dept Syst Anal, Minsk 220050, Byelarus
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Malikova, N.P.; Visser, N.V.; van Hoek, A...; Skakun, V.V.; Vysotski, E.S.; Lee, J...; Visser, AJWG

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Metallic salts action on bacterial bioluminescent systems of different complexity [Текст] / N. S. Kudryasheva [и др.] // Biofizika. - 1996. - Vol. 41, Is. 6. - P. 1264-1269. - Cited References: 12 . - ISSN 0006-3029

РУБ Biophysics

Аннотация: Action of metallic salts on the bacterial bioluminescent systems (1 - water-soluble coupled system luciferase - NADH:FMN-oxydoreductase; 2 - immobilized in starch gel coupled system luciferase - NADH:FMN-oxydoreductase; 3 - intact bacteria) has been studied. The action parameters have been calculated. The effects have been shown to depend on nature of cations but not anions. Electronic structure of cations (nature of external orbitals, charge, radii) has been taken into account. Cations with similar electronic shells nature affect on the row of the bioluminescent systems mentioned above in a similar way. Differences in effects have been explained with diffusion rate, cellular shell and respiration of bacteria.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N.S.; Zuzikova, E.V.; Gutnyk, T.V.; Kuznetsov, A.M.

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Nonequilibrium capillary electrophoresis of equilibrium mixtures, mathematical model [Text] / V. . Okhonin, S. M. Krylova, S. N. Krylov // Anal. Chem. - 2004. - Vol. 76, Is. 5. - P. 1507-1512, DOI 10.1021/ac035259p. - Cited References: 24 . - ISSN 0003-2700

РУБ Chemistry, Analytical
Рубрики:
SURFACE-PLASMON RESONANCE
   PROTEIN-DNA INTERACTIONS
   FLUORESCENCE POLARIZATION
   COMPLEX-FORMATION
   BINDING
   RECOGNITION
   DERIVATIVES
   TOOL
Аннотация: We recently introduced a new electrophoretic method, nonequilibrium, capillary electrophoresis of equilibrium mixtures (NECEEM). NECEEM provides a unique way of finding kinetic and equilibrium parameters of the formation of intermolecular complexes from a single electropherogram and allows for the use of weak affinity probes in protein quantitation. In this work, we study theoretical bases of NECEEM by developing a mathematical model for the new method. By solving a system of partial differential equations with diffusion in linear approximation, we found the analytical solution for concentrations of components involved in complex formation as functions of time from the beginning of separation and position in the capillary. The nonnumerical nature of the solution makes it a powerful tool in studying the theoretical foundations of the NECEEM method and modeling experimental results. We demonstrate the use of the model for finding binding parameters of complex formation by nonlinear regression of NECEEM electropherograms obtained experimentally.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Okhonin, V...; Krylova, S.M.; Krylov, S.N.

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10.

The investigation on the dynamics of frontal zones in the ocean based on the numerical modelling, using the AVHRR satellite data [Text] / A. V. Kartushinsky ; ed. P Schlussel [et al.] // CLIMATE CHANGE PROCESSES IN THE STRATOSPHERE, EARTH-ATMOSPHERE-OCEAN SYSTEMS, AND OCEANOGRAPHIC PROCESSES FROM SATELLITE DATA. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2004. - Vol. 33: 2nd World Space Congress/34th COSPAR Scientific Assembly (OCT 10-19, 2002, HOUSTON, TX), Is. 7. - P. 1173-1178, DOI 10.1016/S0273-1177(03)00370-3. - Cited References: 19 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Environmental Sciences + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences + Remote Sensing
Рубрики:
VARIABILITY
Кл.слова (ненормированные):
frontal temperature zones -- model -- numerical experiments -- current velocity -- turbulent diffusion -- heat flow
Аннотация: The main purpose of the work is to investigate the frontal temperature zones using the mathematical model of the oceanic temperature field. The forecast of the frontal temperature zone variability is based on satellite data. The model calculates the temperature under different starting conditions and allows for the mean monthly intensity of solar radiation, components of current velocities, and turbulent diffusion. The input data are the mean monthly current velocities and the radiation heat flows. The model is used to calculate the dynamics of the frontal temperature zones in separate parts of the ocean. Based on the results of numerical experiments presented in the paper we estimated the spatial and temporal ranges of the frontal zone variation affected by the advection of currents, horizontal turbulent heat exchange, and the radiation heat flow in separate parts of the ocean. As examples we consider the basic frontal zones in the Atlantic and the Pacific Oceans. The work also shows possible applications of parametrization of spatial horizontal components of the frontal zones functionally related to the processes of heat advection and diffusion in the absence of exact information about the magnitudes of the current velocities and turbulent mixing. (C) 2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

WOS
Держатели документа:
Krasnoyarsk State Univ, Inst Biophys, Russian Acad Sci, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kartushinsky, A.V.; Schlussel, P \ed.\; Stuhlmann, R \ed.\; Campbell, JW \ed.\

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11.

Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics / T. G. Volova [et al.] // Polym Test. - 2018. - Vol. 65. - P54-68, DOI 10.1016/j.polymertesting.2017.10.023 . - ISSN 0142-9418
Кл.слова (ненормированные):
Antibacterial activity -- Antibiotics -- Bacterial cellulose -- Composites -- Properties -- Silver nanoparticles -- Antibiotics -- Atoms -- Boron carbide -- Cell culture -- Cellulose -- Cellulose films -- Composite materials -- Escherichia coli -- Materials testing apparatus -- Metal nanoparticles -- Nanocomposite films -- Nanoparticles -- Scanning electron microscopy -- Silver compounds -- Spectrum analysis -- Synthesis (chemical) -- Tensile testing -- Water pollution -- X ray analysis -- Anti-bacterial activity -- Antibacterial properties -- Bacterial cellulose -- Mechanical characteristics -- Properties -- Silver nanoparticles -- Structure and properties -- Tensile testing machines -- Silver -- Antibiotics -- Cellulose -- Composites -- Properties -- Silver
Аннотация: The present study describes production of bacterial cellulose composites with silver nanoparticles and antibiotics and compares their properties. Bacterial cellulose (BC) composites synthesized in the culture of the strain of acetic acid bacterium Komagataeibacter xylinus VKPM B-12068 with silver nanoparticles, BC/AgNps, were produced hydrothermally, under different AgNO3 concentrations (0.0001, 0.001, and 0.01 M) in the reaction medium. The presence of silver in the BC/AgNp composites was confirmed by elemental analysis conducted using scanning electron microscopy with a system of X-ray spectral analysis. Analysis showed that the average atomic number of silver particles in composite samples depended on the concentration of AgNO3: as AgNO3 concentration in the reaction solution was increased, silver content in the composites increased from 0.044 to 0.37 mg/cm2. BC composites with amikacin and ceftriaxone were prepared by immersing dry BC films in solutions containing different concentrations of the antibiotics. The surface structure and properties and physicochemical and mechanical characteristics of composites were investigated using SEM, DSC, X-ray analysis, the system for measuring water contact angles, and electromechanical tensile testing machine. The disk-diffusion method and the shake-flask culture method used in this study showed that all experimental composites had pronounced antibacterial activity against E. coli, Ps. eruginosa, K. pneumoniae, and St. aureus, and the BC/antibiotic composites were more active than BC/AgNp ones; S. aureus was the most susceptible to the effect of BC composites. No potential cytotoxicity was detected in any of the BC/AgNp composites in the NIH 3T3 mouse fibroblast cell culture, in contrast to the BC/antibiotic composites. These results suggest that BC composites constructed in the present study hold promise as dressings for managing wounds, including contaminated ones. © 2017 Elsevier Ltd

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WOS
Держатели документа:
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 43/50 Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Shumilova, A. A.; Shidlovskiy, I. P.; Nikolaeva, E. D.; Sukovatiy, A. G.; Vasiliev, A. D.; Shishatskaya, E. I.

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12.

Biological activity of carbonic nano-structures—comparison via enzymatic bioassay / A. S. Sachkova [et al.] // J. Soils Sed. - 2018, DOI 10.1007/s11368-018-2134-9 . - Article in press. - ISSN 1439-0108
Кл.слова (ненормированные):
Antioxidant activity -- Bioactive compounds -- Fullerenol -- Humic substances -- Reactive oxygen species -- Toxicity
Аннотация: Purpose: The aim of the work is to compare the biological activity of carbonic nano-structures of natural and artificial origination, namely, humic substances (HS) and fullerenols. Materials and methods: The representative of the fullerenol group, С60Оy(OH)x where у + x = 20–22, was chosen. Enzyme-based luminescent bioassay was applied to evaluate toxicity and antioxidant properties of HS and fullerenol (F); chemiluminescent luminol method was used to study a content of reactive oxygen species (ROS) in the solutions. Toxicity of the bioactive compounds was evaluated using effective concentrations ЕС50; detoxification coefficients DOxT were applied to study and compare antioxidant activity of the compounds. Antioxidant activity and ranges of active concentrations of the bioactive compounds were determined in model solutions of organic and inorganic oxidizers—1,4-benzoquinone and potassium ferricianide. Results and discussion: Values of ЕС50 revealed higher toxicity of HS than F (0.005 and 0.108 g L?1, respectively); detoxifying concentrations of F were found to be lower. Antioxidant ability of HS was demonstrated to be time-dependent; the 50-min preliminary incubation in oxidizer solutions was suggested as optimal for the detoxification procedure. On the contrary, F’ antioxidant effect demonstrated independency on time. Antioxidant effect of HS did not depend on amphiphilic characteristics of the media (values of DOxT were 1.3 in the solutions of organic and inorganic oxidizers), while this of F was found to depend: it was maximal (DOxT = 2.0) in solutions of organic oxidizer, 1,4-benzoquinone. Conclusions: Both HS and F demonstrated toxicity and low-concentration antioxidant ability; however, quantitative characteristics of their effects were different. The differences were explained with HS polyfunctionality, higher ability to decrease ROS content, non-rigidity, and diffusion restrictions in their solutions. Antioxidant effect of the bioactive compounds was presumably attributed to catalytic redox activity of their ?-fragments. The paper demonstrates a high potential of luminescent enzymatic bioassay to study biological activity of nano-structures of natural and artificial origination. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

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Держатели документа:
National Research Tomsk Polytechnic University, Tomsk, 634050, Russian Federation
Institute of Biophysics FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Physics FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Irkutsk National Research Technical University, Irkutsk, 664074, Russian Federation

Доп.точки доступа:
Sachkova, A. S.; Kovel, E. S.; Churilov, G. N.; Stom, D. I.; Kudryasheva, N. S.

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13.

Properties of Bacterial Cellulose Composites with Silver Nanoparticles / I. P. Shidlovskiy [et al.] // Biophysics. - 2018. - Vol. 63, Is. 4. - P519-525, DOI 10.1134/S0006350918040188 . - ISSN 0006-3509
Кл.слова (ненормированные):
:bacterial cellulose -- antibacterial activity -- composites -- hydrothermal synthesis -- silver nanoparticles
Аннотация: Abstract: Composites of bacterial cellulose, which were synthesized in a culture of the strain of acetic acid bacteria Komagataeibacter xylinus VKPM B-12068, with silver nanoparticles were produced hydrothermally by varying the concentrations of AgNO3 in the medium. The presence of silver in the composites was confirmed by elemental analysis. An increase in the number of silver nanoparticles in the composite from 1.08 to 9.1 wt % (from 0.044 to 0.370 mg/cm2) was shown under increasing AgNO3 concentration in the medium from 0.0001 to 0.01 M. The structure, properties of the surface, and the physicochemical properties of the composites depending on the silver content were investigated using scanning electron microscopy, differential scanning calorimetry, X-ray diffraction, and a water contact-angle measurement system. Using the disk-diffusion method, it was shown that the resulting composites have a pronounced antibacterial activity against pathogenic microflora E. coli, Ps. eruginosa, and St. aureus. © 2018, Pleiades Publishing, Inc.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Shidlovskiy, I. P.; Shumilova, A. A.; Shishatskaya, E. I.; Volova, T. G.

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14.

Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction / A. E. Lisitsa, L. A. Sukovatyi, V. A. Kratasyuk, E. V. Nemtseva // Doklad. Biochem. Biophys. - 2020. - Vol. 492, Is. 1. - P162-165, DOI 10.1134/S1607672920020106 . - ISSN 1607-6729
Кл.слова (ненормированные):
bacterial luciferase -- bioluminescence -- diffusional restriction -- enzyme reaction intermediate -- molecular dynamics method -- stopped-flow technique -- viscous microenvironment
Аннотация: Abstract: The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1–1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in the decay rate following a power function with an exponent of 0.82–0.84. Using molecular dynamics methods, it was shown that the presence of glycerol and sucrose molecules causes a change in the mobility of the amino acid residues in the active center of luciferase, particularly those responsible for binding of flavin. The results obtained are indicative of two opposite effects of viscous media with glycerol and sucrose: (1) destabilization of 4a-hydroperoxyflavin due to a change in the structural and dynamic properties of the protein and (2) stabilization of this intermediate by the decrease in the diffusion rate of its decay products. © 2020, Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, AkademgorodokKrasnoyarsk, Russian Federation

Доп.точки доступа:
Lisitsa, A. E.; Sukovatyi, L. A.; Kratasyuk, V. A.; Nemtseva, E. V.

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15.

Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction / A. E. Lisitsa, L. A. Sukovatyi, V. A. Kratasyuk, E. V. Nemtseva // Dokl. Biochem. Biophys. - 2020. - Vol. 492, Is. 1. - P162-165, DOI 10.1134/S1607672920020106. - Cited References:15. - This work was supported by the Ministry of Science and Higher Education of the Russian Federation (project nos. 6.7734.2017 and 01201351504). . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
LUCIFERASE
Кл.слова (ненормированные):
bacterial luciferase -- bioluminescence -- viscous microenvironment -- stopped-flow technique -- molecular dynamics method -- enzyme reaction -- intermediate -- diffusional restriction
Аннотация: The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1-1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in the decay rate following a power function with an exponent of 0.82-0.84. Using molecular dynamics methods, it was shown that the presence of glycerol and sucrose molecules causes a change in the mobility of the amino acid residues in the active center of luciferase, particularly those responsible for binding of flavin. The results obtained are indicative of two opposite effects of viscous media with glycerol and sucrose: (1) destabilization of 4a-hydroperoxyflavin due to a change in the structural and dynamic properties of the protein and (2) stabilization of this intermediate by the decrease in the diffusion rate of its decay products.

WOS
Держатели документа:
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.

Доп.точки доступа:
Lisitsa, A. E.; Sukovatyi, L. A.; Kratasyuk, V. A.; Nemtseva, E. V.; Ministry of Science and Higher Education of the Russian Federation [6.7734.2017, 01201351504]

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16.

    A Novel Approach Towards Green Synthesis of Nanodiamonds as Biocompatible Agents / A. Anand, M. Saran, S. Chaudhary [et al.] // J. Nano. Electron. Phys. - 2021. - Vol. 13, Is. 3. - P1-6, DOI 10.21272/jnep.13(3).03040 . - ISSN 2077-6772
   Перевод заглавия: Новий підхід до зеленого синтезу наноалмазів як біосумісних агентів
Кл.слова (ненормированные):
Antimicrobial -- Antioxidants -- Biocompatible agents -- Green synthesis
Аннотация: The application of nanobiotechnology is an emerging area of nanoscience and nanotechnology. Nanodi-amond has been a potent antibacterial, antifungal, antioxidant, and antiplatelet agent. In the present study, nanodiamonds were reduced by green synthesis and characterization was done through SEM, TEM, FTIR, and XRD. Further they were tested for their biological applications. The antimicrobial activity was investigated/studied/examined? through well diffusion method. The best activity was observed against Trichoderma reesei (16 mm) at 140 ?g/ml. The antioxidant activity was investigated through DPPH and FRAPS method. It was observed that the biologically reduced nanodiamonds reduce the Fe3+ ions to Fe2+ ions at 600 mM/l/g concentration. In DPPH assay, inhibitory concentration was found to be 4.58 ?g/ml. Further the antiplatelet activity was investigated by prothrombin time (PT) and activated partial throm-boplastin time (APTT) assay, and it was observed that biologically reduced nanodiamonds have potent an-tiplatelet activity. © 2021 Sumy State University

Scopus
Держатели документа:
Department of Biotechnology, Mewar University, Chittorgarh, 312901, India
Department of Physics, Manipal University Jaipur, Jaipur, 303007, India
Department of Nanobiotechnology, Seminal Applied Sciences Pvt. Ltd, Jaipur, 302015, India
Department of Chemistry, Malviya National Institute of Technology, Jaipur, 302017, India
School of Agriculture, Suresh Gyan Vihar University, Mahal Road, Jaipur, 302017, India
Institute of Computational Technologies SB RAS, Krasnoyarsk, 660049, Russian Federation
Institute of Biophysics, SB RAS, Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Anand, A.; Saran, M.; Chaudhary, S.; Ronin, R. S.; Swami, A. K.; Mathur, M.; Burov, A.; Bagaria, A.

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17.

Mechanisms of viscous media effects on elementary steps of bacterial bioluminescent reaction / A. E. Lisitsa, L. A. Sukovatyi, S. I. Bartsev [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 16. - Ст. 8827, DOI 10.3390/ijms22168827 . - ISSN 1661-6596
Кл.слова (ненормированные):
Bacterial luciferase -- Diffusion limitation -- Non-steady-state reaction kinetics -- Viscosity
Аннотация: Enzymes activity in a cell is determined by many factors, among which viscosity of the microenvironment plays a significant role. Various cosolvents can imitate intracellular conditions in vitro, allowing to reduce a combination of different regulatory effects. The aim of the study was to analyze the media viscosity effects on the rate constants of the separate stages of the bacterial biolumi-nescent reaction. Non-steady-state reaction kinetics in glycerol and sucrose solutions was measured by stopped-flow technique and analyzed with a mathematical model developed in accordance with the sequence of reaction stages. Molecular dynamics methods were applied to reveal the effects of cosolvents on luciferase structure. We observed both in glycerol and in sucrose media that the stages of luciferase binding with flavin and aldehyde, in contrast to oxygen, are diffusion-limited. More-over, unlike glycerol, sucrose solutions enhanced the rate of an electronically excited intermediate formation. The MD simulations showed that, in comparison with sucrose, glycerol molecules could penetrate the active-site gorge, but sucrose solutions caused a conformational change of functionally important ?Glu175 of luciferase. Therefore, both cosolvents induce diffusion limitation of substrates binding. However, in sucrose media, increasing enzyme catalytic constant neutralizes viscosity effects. The activating effect of sucrose can be attributed to its exclusion from the catalytic gorge of luciferase and promotion of the formation of the active site structure favorable for the catalysis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Biophysics Department, Siberian Federal University, Svobodny 79, Krasnoyarsk, 660041, Russian Federation
The Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Lisitsa, A. E.; Sukovatyi, L. A.; Bartsev, S. I.; Deeva, A. A.; Kratasyuk, V. A.; Nemtseva, E. V.

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18.

Mechanisms of Viscous Media Effects on Elementary Steps of Bacterial Bioluminescent Reaction / A. E. Lisitsa, L. A. Sukovatyi, S. I. Bartsev [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 16. - Ст. 8827, DOI 10.3390/ijms22168827. - Cited References:59. - The research was funded by the Ministry of Science and Higher Education of the Russian Federation (projects No. FSRZ-2020-0006); by RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science (project No. 20-44-243002); by RFBR according to the research project No. 20-34-90118. . - ISSN 1422-0067

РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
FLAVIN INTERMEDIATE
   REDUCED FLAVIN
   RATE CONSTANTS
   LUCIFERASE
Кл.слова (ненормированные):
bacterial luciferase -- non-steady-state reaction kinetics -- viscosity -- diffusion limitation
Аннотация: Enzymes activity in a cell is determined by many factors, among which viscosity of the microenvironment plays a significant role. Various cosolvents can imitate intracellular conditions in vitro, allowing to reduce a combination of different regulatory effects. The aim of the study was to analyze the media viscosity effects on the rate constants of the separate stages of the bacterial bioluminescent reaction. Non-steady-state reaction kinetics in glycerol and sucrose solutions was measured by stopped-flow technique and analyzed with a mathematical model developed in accordance with the sequence of reaction stages. Molecular dynamics methods were applied to reveal the effects of cosolvents on luciferase structure. We observed both in glycerol and in sucrose media that the stages of luciferase binding with flavin and aldehyde, in contrast to oxygen, are diffusion-limited. Moreover, unlike glycerol, sucrose solutions enhanced the rate of an electronically excited intermediate formation. The MD simulations showed that, in comparison with sucrose, glycerol molecules could penetrate the active-site gorge, but sucrose solutions caused a conformational change of functionally important alpha Glu175 of luciferase. Therefore, both cosolvents induce diffusion limitation of substrates binding. However, in sucrose media, increasing enzyme catalytic constant neutralizes viscosity effects. The activating effect of sucrose can be attributed to its exclusion from the catalytic gorge of luciferase and promotion of the formation of the active site structure favorable for the catalysis.

WOS
Держатели документа:
Siberian Fed Univ, Biophys Dept, Svobodny 79, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Akad Gorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Lisitsa, Albert E.; Sukovatyi, Lev A.; Bartsev, Sergey, I; Deeva, Anna A.; Kratasyuk, Valentina A.; Nemtseva, Elena, V; Nemtseva, Elena; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science [20-44-243002]; RFBRRussian Foundation for Basic Research (RFBR) [20-34-90118]

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19.

Hydrochemical Indicators of Water Quality in the Norilsk-Pyasino Lake-River System after a Diesel Fuel Spill at Norilsk Heat and Power Plant 3 in 2020 / D. M. Bezmaternykh, A. V. Puzanov, A. V. Kotovshchikov [et al.] // Contemp. Probl. Ecol. - 2021. - Vol. 14, Is. 4. - P323-334, DOI 10.1134/S1995425521040028. - Cited References:22 . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology

Кл.слова (ненормированные):
hydrochemistry -- water quality -- Pyasino River -- Lake Pyasino -- Norilsk -- diesel fuel
Аннотация: The results of a hydrochemical analysis of the consequences of an accidental fuel release in the Norilsk-Pyasino water system are presented. The pollution of watercourses in the catchment of Lake Pyasino (a nameless (Nadezhdinsky) creek, the Daldykan River, and the Ambarnaya River) with oil products, phenols, easily oxidizable and hard to oxidize organic matter (COD, PO, and BOD5), suspended solids, inorganic salts, and heavy metals at concentrations exceeding the background levels and MPC for fishery water bodies, as well as a temperature rise in waters of the nameless creek near Norilsk Heat and Power Plant 3 (CHPP-3), have been revealed. The contamination of the surface water decreases downstream in ascending order: nameless creek-Daldykan River-Ambarnaya River. The occurrence of oil products, phenols, and organic substances in the surface waters 2 months after the fuel spill is obviously due to their diffusion from the river bottom sediments, which accumulated a considerable quantity of heavy fractions of diesel fuel after the accident. Increased concentrations of Ca, Cu, Zn, Mn, Co, and Ni in the waters of the studied tributaries of Lake Pyasino are not directly related to the accident; they result from the general technogenic pollution of the territory and the increased geochemical background for these elements. Water contamination with oil products and phenols in the studied areas of Lake Pyasino (its central and northern parts) and the Pyasino River has not been detected. However, Pb concentrations exceed the MPC and Cd is recorded in the water, which is probably due to pollutants that accumulated in previous years.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Water & Environm Problems, Barnaul 656038, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Bezmaternykh, D. M.; Puzanov, A. V.; Kotovshchikov, A. V.; Drobotov, A. V.; Tolomeev, A. P.

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20.

Hydrochemical Indicators of Water Quality in the Norilsk–Pyasino Lake–River System after a Diesel Fuel Spill at Norilsk Heat and Power Plant 3 in 2020 / D. M. Bezmaternykh, A. V. Puzanov, A. V. Kotovshchikov [et al.] // Contemp. Probl. Ecol. - 2021. - Vol. 14, Is. 4. - P323-334, DOI 10.1134/S1995425521040028 . - ISSN 1995-4255
Кл.слова (ненормированные):
diesel fuel -- hydrochemistry -- Lake Pyasino -- Norilsk -- Pyasino River -- water quality
Аннотация: Abstract: The results of a hydrochemical analysis of the consequences of an accidental fuel release in the Norilsk–Pyasino water system are presented. The pollution of watercourses in the catchment of Lake Pyasino (a nameless (Nadezhdinsky) creek, the Daldykan River, and the Ambarnaya River) with oil products, phenols, easily oxidizable and hard to oxidize organic matter (COD, PO, and BOD5), suspended solids, inorganic salts, and heavy metals at concentrations exceeding the background levels and MPC for fishery water bodies, as well as a temperature rise in waters of the nameless creek near Norilsk Heat and Power Plant 3 (CHPP-3), have been revealed. The contamination of the surface water decreases downstream in ascending order: nameless creek–Daldykan River–Ambarnaya River. The occurrence of oil products, phenols, and organic substances in the surface waters 2 months after the fuel spill is obviously due to their diffusion from the river bottom sediments, which accumulated a considerable quantity of heavy fractions of diesel fuel after the accident. Increased concentrations of Ca, Cu, Zn, Mn, Co, and Ni in the waters of the studied tributaries of Lake Pyasino are not directly related to the accident; they result from the general technogenic pollution of the territory and the increased geochemical background for these elements. Water contamination with oil products and phenols in the studied areas of Lake Pyasino (its central and northern parts) and the Pyasino River has not been detected. However, Pb concentrations exceed the MPC and Cd is recorded in the water, which is probably due to pollutants that accumulated in previous years. © 2021, Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute for Water and Environmental Problems, Siberian Branch, Russian Academy of Sciences, Barnaul, 656038, Russian Federation
Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Bezmaternykh, D. M.; Puzanov, A. V.; Kotovshchikov, A. V.; Drobotov, A. V.; Tolomeev, A. P.

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