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Seasonal features of consumption of lysine by uncultivated bacterial plankton of Eutrophic water reservoir / M. Y. Trusova, O. V. Kolmakova, M. I. Gladyshev // Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - P391-398, DOI 10.1134/S1995425512040154 . - ISSN 1995-4255
Кл.слова (ненормированные):
16S ribosomal RNA -- biogeochemical function -- denaturing gradient gel electrophoresis -- eutrophic water reservoir -- lysine -- microecosystem (MES) -- uncultivated bacterial plankton -- amino acid -- bacterioplankton -- bacterium -- biogeochemistry -- electrokinesis -- eutrophication -- laboratory method -- microbial community -- polymerase chain reaction -- reservoir -- seasonal variation -- specialization -- Bacteria (microorganisms)
Аннотация: Dynamics of bacterial plankton community of eutrophic water reservoir in laboratory microecosystems with amino acid lysine was studied using PCR-DGGE technique. The addition of lysine to the microecosystems resulted in changes in the composition of the bacterial plankton in summer; in particular, a number of Lys1 and Lys2 species (genotypes) that consume this amino acid grew fast in the bacterial community. The plank tonic bacterial communities did not respond to the addition of lysine in spring and late summer. The obtained data confirm the hypothesis for the narrow specialization of bacterial plankton species to the consumption of individual organic substances. В© 2012 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok 50-50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnii prosp. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Y.; Kolmakova, O.V.; Gladyshev, M.I.

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Surface properties of nanodiamond films deposited by electrophoresis on Si(100) / E. Maillard-Schaller [et al.] // Diamond and Related Materials. - 1999. - Vol. 8, Is. 2-5. - P805-808 . - ISSN 0925-9635
Кл.слова (ненормированные):
Energy band diagram -- Nanodiamond -- Raman spectroscopy -- Surface characterization -- Band structure -- Electrodeposition -- Electrophoresis -- Hydrogen -- Nanostructured materials -- Nitrogen -- Oxidation -- Oxygen -- Phonons -- Plasma applications -- Silicon wafers -- Surface properties -- Dielectrophoresis -- Negative electron affinity (NEA) -- Phonon confinement effect -- Diamond films
Аннотация: The surface properties of diamond nanoparticles (40-50 A in diameter) have been investigated by X-ray photoelectron spectroscopy (XPS), UV photoelectron spectroscopy (UPS) and Raman spectroscopy. The diamond nanoparticles have been deposited on flat Si(100) substrates by electrophoresis/dielectrophoresis. The as-deposited films are strongly oxidized and present a 1-2% nitrogen content. After treatment at 850 В°C in H2 plasma for 60 min, the oxygen is removed, and the position of the C 1s core-level peak indicates a n-type electronic comportment of the diamond nanoparticles. Raman spectroscopy of the as-deposited film shows a sp3 contribution at 1321 cm-1 and a sp2 contribution around 1620 cm-1. The 12 cm-1 shift of the sp3 contribution with respect to the bulk diamond peak at 1333 cm-1 is attributed to a phonon confinement effect due to the size of the diamond particles. The H2 plasma treatment induces a size decrease of the nanocrystallites confirmed by Raman and scanning electron microscopy (SEM) measurements. UPS spectroscopy shows a negative electron affinity of -0.2 eV of the hydrogenated nanodiamond film.

Scopus
Держатели документа:
Solid State Physics Department, University of Fribourg, 1700, Fribourg, Switzerland
Institute of Christallography, 117333, Moscow, Russian Federation
Institute of Biophysics, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maillard-Schaller, E.; Kuettel, O.M.; Diederich, L.; Schlapbach, L.; Zhirnov, V.V.; Belobrov, P.I.

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Electrophoresis of nanodiamond powder for cold cathode fabrication [Text] / A. N. Alimova [et al.] // J. Vac. Sci. Technol. B. - 1999. - Vol. 17: 11th International Vacuum Microelectronics Conference (IVMC 98) (JUL 19-23, 1998, ASHEVILLE, NORTH CAROLINA), Is. 2. - P715-718, DOI 10.1116/1.590625. - Cited References: 5 . - 4. - ISSN 1071-1023

РУБ Engineering, Electrical & Electronic + Nanoscience & Nanotechnology + Physics, Applied
Рубрики:
FIELD-EMISSION
TIPS
Аннотация: The process of electrophoresis/dielectrophoresis of shock-synthesized nanodiamond powders on the large-area Si field emission arrays was studied. The process was found to be very sensitive to the modification of nanodiamond powder. The growth rate and film morphology of nanodiamond coatings on Si tips were investigated. Results in fabrication and testing of nanodiamond-based cold cathodes and sealed device prototypes are presented. (C) 1999 American Vacuum Society. [S0734-211X(99)06002-3].

Держатели документа:
Russian Acad Sci, Inst Crystallog, Moscow 117333, Russia
ISTOK, State Res & Prod Corp, Fryazino 141120, Moscow Region, Russia
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
Russian Fed Nucl Ctr, Snezhinsk 456770, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Alimova, A.N.; Chubun, N.N.; Belobrov, P.I.; Detkov, P.Y.; Zhirnov, V.V.

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PHYSICOCHEMICAL PROPERTIES OF A PHOTOPROTEIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA [Text] / V. S. BONDAR, K. P. TROFIMOV, E. S. VYSOTSKII // Biochem.-Moscow. - 1992. - Vol. 57, Is. 10. - P1020-1027. - Cited References: 36 . - 8. - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology
Рубрики:
CALCIUM-ACTIVATED PHOTOPROTEINS
   CTENOPHORES MNEMIOPSIS SP
   BEROE-OVATA
   AEQUORIN
   CA-2+
   INDICATORS
   PROTEIN
   BINDING
   PURIFICATION
   EXTRACTION
Кл.слова (ненормированные):
BIOLUMINESCENCE -- CA2+-ACTIVATED PHOTOPROTEIN -- OBELIN -- CHROMATOGRAPHY -- CALCIUM
Аннотация: The photoprotein obelin was isolated and purified to homogeneity (as indicated by sodium dodecyl sulfate polyacrylamide gel electrophoresis) from hydroids of Obelia longissima by gel filtration on Sephadex G-75 fine, ion exchange chromatography on Polysil CA-300 (10 mum), hydrophobic chromatography on Phenyl-Sepharose CL-4B, gel filtration on Sephacryl S-200 superfine, ion exchange chromatography on a Mono Q column at pH 7.0, chromatofocusing on a Mono P column (pH gradient 6.0-4.0), and ion exchange chromatography on a Mono Q column at pH 5.5, 8.8, and 7.0. The molecular weight of the native protein was 30 kD, and that measured in the presence of SDS was 19.8 kD. The specific activity of obelin is 4.9.10(15) quanta/mg protein, pseudo-first-order constant of bioluminescence decay 4 sec-1, and quantum yield 0.16 The range of measurable Ca2+ concentrations is 10(-7) to 10(-5) M. The luminescence spectrum of obelin peaks at 469 nm, and the fluorescence emission maximum of the discharged protein is at 455 nm. The optimum pH for luminescence is between 9.0 and 10.5. The molecular ionization constants are pK1 6.8 and pK2 12.2, and the ionization constants for the active site are pK1 9.1 and pK2 10.2
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
BONDAR, V.S.; TROFIMOV, K.P.; VYSOTSKII, E.S.

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Physical and chemical properties of modified nanodiamonds [Text] / A. P. Puzyr [et al.] ; ed.: DM Gruen, OA Shenderova, erova, OA She // Synthesis, Properties and Applications of Ultrananocrystalline Diamond. Ser. NATO SCIENCE SERIES, SERIES II: MATHEMATICS, PHYSICS AND CHEMISTRY : SPRINGER, 2005. - Vol. 192: NATO Advanced Research Workshop on Synthesis, Properties and Applications of Ultrananocrystalline Diamond (JUN 07-10, 2004, St Petersburg, RUSSIA). - P261-270. - Cited References: 15 . - ISBN 1-4020-3320-6

РУБ Chemistry, Physical + Materials Science, Multidisciplinary

Кл.слова (ненормированные):
nanodiamond -- modified nanodiamonds -- hydrosols -- colloidal stability -- electrophoresis -- powder fractioning -- precursors for CVD -- electron field emission
Аннотация: A unique technology of nanodiamond surface modification is suggested which allows to separation of commercial nanodiamond powders into two fractions (F1 and 172), each possessing absolutely new properties as compared to the initial powder. F1 and F2 differ in size characteristics. Initial and modified nanodiamonds contain iron impurities and two types of nondiamond carbon. The color of the powders and hydrosols does not correlate with the content of non-diamond carbon. According to the EPR data, modified nanodiamonds possess a high level of diamond matrix shielding, and the extracted fractions differ in width of the basic transition area and in the SHF energy adsorption ratio. Due to this, F1 can be applied as precursors for CVD growth of nanocrystalline diamond and as field electron emission tips.

Держатели документа:
RAS, Inst Biophys SB, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr, A.P.; Bondar, V.S.; Bukayemsky, A.A.; Selyutin, G.E.; Kargin, V.F.; Gruen, DM \ed.\; Shenderova, OA \ed.\; She, erova, OA \ed.\

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ELECTROPHORETIC BEHAVIOR OF HYDROSOLS OF ULTRADISPERSE DIAMOND AND MODIFICATION OF ITS SURFACE [Text] / G. A. CHIGANOVA, V. A. BONDAR, A. S. CHIGANOV // COLLOID JOURNAL OF THE RUSSIAN ACADEMY OF SCIENCES. - 1993. - Vol. 55, Is. 5. - P. 774-775. - Cited References: 5 . - ISSN 1061-933X

РУБ Chemistry, Physical

Аннотация: The method of macro-electrophoresis is used to determine values of the zeta-potential of particles of ultradisperse diamond in solutions of AlCl3. It is shown that an increase in the concentration of aluminum ions to 0.5.10(-4) M leads to a decrease in the negative charge of the diamond particles; the dispersions coagulate in more concentrated solutions of AlCl3. Chemical modification of the surface of ultradisperse diamond by aluminum ions is used to obtain stable hydrosols with positively charged diamond particles.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
CHIGANOVA, G.A.; BONDAR, V.A.; CHIGANOV, A.S.

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Nonequilibrium capillary electrophoresis of equilibrium mixtures, mathematical model [Text] / V. . Okhonin, S. M. Krylova, S. N. Krylov // Anal. Chem. - 2004. - Vol. 76, Is. 5. - P. 1507-1512, DOI 10.1021/ac035259p. - Cited References: 24 . - ISSN 0003-2700

РУБ Chemistry, Analytical
Рубрики:
SURFACE-PLASMON RESONANCE
   PROTEIN-DNA INTERACTIONS
   FLUORESCENCE POLARIZATION
   COMPLEX-FORMATION
   BINDING
   RECOGNITION
   DERIVATIVES
   TOOL
Аннотация: We recently introduced a new electrophoretic method, nonequilibrium, capillary electrophoresis of equilibrium mixtures (NECEEM). NECEEM provides a unique way of finding kinetic and equilibrium parameters of the formation of intermolecular complexes from a single electropherogram and allows for the use of weak affinity probes in protein quantitation. In this work, we study theoretical bases of NECEEM by developing a mathematical model for the new method. By solving a system of partial differential equations with diffusion in linear approximation, we found the analytical solution for concentrations of components involved in complex formation as functions of time from the beginning of separation and position in the capillary. The nonnumerical nature of the solution makes it a powerful tool in studying the theoretical foundations of the NECEEM method and modeling experimental results. We demonstrate the use of the model for finding binding parameters of complex formation by nonlinear regression of NECEEM electropherograms obtained experimentally.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Okhonin, V...; Krylova, S.M.; Krylov, S.N.

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    Biological and Ecological Features, Trophic Structure and Energy Flow in Meromictic Lakes / E. S. Zadereev, R. D. Gulati, A. Camacho ; ed.: R. D. Gulati, E. S. Zadereev, A. G. Degermendzhi // Ecol. Stud. : SPRINGER INTERNATIONAL PUBLISHING AG, 2017. - Vol. 228. - P61-86. - (Ecological Studies-Analysis and Synthesis), DOI 10.1007/978-3-319-49143-1_4. - Cited References:98
Рубрики:
PHOTOTROPHIC SULFUR BACTERIA
   GRADIENT GEL-ELECTROPHORESIS
   16S

WOS,
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Держатели документа:
Inst Biophys SB RAS, Krasnoyarsk, Russia.
Netherlands Inst Ecol NIOO, NL-6708 PB Wageningen, Netherlands.
Univ Valencia, Cavanilles Inst Biodivers & Evolutionary Biol, E-46100 Valencia, Spain.
Univ Valencia, Dept Microbiol & Ecol, E-46100 Valencia, Spain.

Доп.точки доступа:
Zadereev, Egor S.; Gulati, Ramesh D.; Camacho, Antonio; Gulati, R.D. \ed.\; Zadereev, E.S. \ed.\; Degermendzhi, A.G. \ed.\

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