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Assessment of the rate of accumulation of the transuranium element americium-241 by the aquatic plant Elodea canadensis / A. Ya. Bolsunovskii [et al.] // Doklady Biological Sciences. - 2004. - Vol. 399, Is. 1-6. - P467-469, DOI 10.1007/s10630-005-0014-0 . - ISSN 0012-4966
Кл.слова (ненормированные):
americium -- radioisotope -- water -- article -- biomass -- drug effect -- environmental monitoring -- Hydrocharitaceae -- methodology -- pH -- plant -- plant root -- radiation exposure -- radiochemistry -- time -- toxicity testing -- water pollutant -- Americium -- Biomass -- Environmental Monitoring -- Hydrocharitaceae -- Hydrogen-Ion Concentration -- Plant Roots -- Plant Shoots -- Plants -- Radiochemistry -- Radioisotopes -- Time Factors -- Toxicity Tests -- Water -- Water Pollutants, Radioactive -- Elodea canadensis

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Ya.; Zotina, T.A.; Bondareva, L.G.; Degermendzhi, A.G.

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Transfer of americium-241 from food and water to organs and tissues of the crucian carp * / T. Zotina [et al.] // Radioprotection. - 2011. - Vol. 46, Is. 6 SUPPL. - S69-S73, DOI 10.1051/radiopro/20116648s . - ISSN 0033-8451
Аннотация: The transfer of transuranium element americium-241 (241Am) from food and water to organs and tissues of freshwater fish (Carassius auratus gibelio, crucian carp) has been investigated in laboratory experiments. The fishes were fed with homogenized biomass of submerged macrophytes labeled with 241Am, through catheter. For the first two days after force-feeding, fishes excreted up to 80% of ingested americium, for four days - 98%. 241Am was registered in internal organs and tissues that had no direct contact with americium (liver, bones, muscles) as early as two days after the feeding and after eight days, when the digestive tract was depurated. Among internal organs, the highest activity concentration was recorded in the liver (up to 427 Bq/kg). Activity concentration in bones (19-31 Bq/kg) was several times higher than in muscles, indicating the affinity of americium to bone tissue. Assimilation of 241Am in organs and tissues of crucian carp from water occurred mainly via the digestive tract too. В© 2011 EDP Sciences.

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Держатели документа:
Institute of Biophysics, SB RAS, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.; Trofimova, E.; Dementyev, D.; Bolsunovsky, A.

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Chemical fractionation of radionuclides and stable elements in aquatic plants of the yenisei river / A. Bolsunovsky // Environmental Science and Technology. - 2011. - Vol. 45, Is. 17. - P7143-7150, DOI 10.1021/es2008853 . - ISSN 0013-936X
Кл.слова (ненормированные):
Aquatic plants -- Artificial radionuclides -- Cell structure -- Chemical Fractionation -- Distribution patterns -- Nuclear plant -- Plant biomass -- Sequential extraction scheme -- Stable elements -- Submerged plants -- Transuranic elements -- Americium -- Biomass -- Neptunium -- River pollution -- Rivers -- Radioisotopes -- americium -- element -- neptunium -- radioisotope -- bioaccumulation -- chemical binding -- gamma ray spectrometry -- isotopic fractionation -- monocotyledon -- phytomass -- radionuclide -- river pollution -- submerged vegetation -- aquatic species -- article -- Batrachium kauffmanii -- biomass -- cell structure -- controlled study -- extraction -- Fontinalis antipyretica -- fractionation -- gamma spectrometry -- higher plant -- nonhuman -- plant growth -- Potamogeton lucens -- Potamogetonaceae -- river -- Russian Federation -- Aquatic Organisms -- Biomass -- Chemical Fractionation -- China -- Industrial Waste -- Plants -- Radiation Monitoring -- Radioisotopes -- Rivers -- Water Pollutants, Radioactive -- Russian Federation -- Yenisei River -- Batrachium -- Fontinalis antipyretica -- Potamogeton lucens
Аннотация: The Yenisei River is contaminated with artificial radionuclides released by one of the Russian nuclear plants. The aquatic plants growing in the radioactively contaminated parts of the river contain artificial radionuclides. The aim of the study was to investigate accumulation of artificial radionuclides and stable elements by submerged plants of the Yenisei River and estimate the strength of their binding to plant biomass by using a new sequential extraction scheme. The aquatic plants sampled were: Potamogeton lucens, Fontinalis antipyretica, and Batrachium kauffmanii. Gamma-spectrometric analysis of the samples of aquatic plants has revealed more than 20 radionuclides. We also investigated the chemical fractionation of radionuclides and stable elements in the biomass and rated radionuclides and stable elements based on their distribution in biomass. The greatest number of radionuclides strongly bound to biomass cell structures was found for Potamogeton lucens and the smallest for Batrachium kauffmanii. For Fontinalis antipyretica, the number of distribution patterns that were similar for both radioactive isotopes and their stable counterparts was greater than for the other studied species. The transuranic elements 239Np and 241Am were found in the intracellular fraction of the biomass, and this suggested their active accumulation by the plants. В© 2011 American Chemical Society.

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Держатели документа:
Institute of Biophysics SB, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.

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Experimental investigations of 241Am accumulation by macrophytes of the Yenisei River / A. Ya. Bolsunovskij, T. A. Zotina // Radiatsionnaya Biologiya. Radioekologiya. - 2002. - Vol. 42, Is. 2. - С. 200-203 . - ISSN 0869-8031
Кл.слова (ненормированные):
Americium -- Aquaculture -- Biomass -- Ecosystems -- Radioisotopes -- Americium 241 -- Macrophytes -- Radioecological concentration -- The Yenisei river -- River pollution -- americium -- absorption -- article -- biomass -- chemistry -- comparative study -- gamma spectrometry -- metabolism -- plant -- Russian Federation -- time -- water pollutant -- Absorption -- Americium -- Biomass -- Plants -- Siberia -- Spectrometry, Gamma -- Time Factors -- Water Pollutants, Radioactive
Аннотация: Experiments were carried out in which 241Am was added to water samples containing macrophytes of the Yenisei River, and the radionuclide absorption rates and concentration factors were determined for the plants. It has been shown that the water moss (Fontinalis antipyretica) has a higher capacity to accumulate 241Am than the Canadian pondweed (Elodea canadensis) does. The laboratory experiments revealed that the capacity of dead biomass of the Canadian pondweed to accumulate 241Am is twice higher than that of living biomass. In contrast, no significant increase in 241Am accumulation by dead biomass of the water moss has been recorded. The transuranic element 241Am was firmly fixed by the plant biomass and was not released into water in the course of long-duration experiments.

Scopus
Держатели документа:
Inst. of Biophysics, Siberian Branch, RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskij, A.Ya.; Zotina, T.A.

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Increased BLSS closure using mineralized human waste in plant cultivation on a neutral substrate / S. Ushakova [et al.] // Advances in Space Research. - 2009. - Vol. 44, Is. 8. - P971-978, DOI 10.1016/j.asr.2009.06.003 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biological life support system -- Crops -- Human waste recycling -- Hydroponics -- Biological life support system -- Biological life support systems -- Biological productivity -- Control experiments -- Control plants -- Environmental conditions -- Expanded clay -- Human waste -- Human waste recycling -- Hydroponics -- In-plants -- Macro element -- Mineral element -- Neutral solution -- Nutrient solution -- Photosynthetic apparatus -- Pisum sativum -- Plant growth -- Scale potential -- Spring wheat -- Triticum aestivum -- Vegetation periods -- Water extracts -- Wheat straws -- Clay minerals -- Crops -- Minerals -- Mining -- Nutrients -- Pilot plants -- Plant life extension -- Potassium -- Productivity -- Recycling -- Solvent extraction -- Vegetation -- Water content -- Plant shutdowns
Аннотация: The purpose of this work was to study the full-scale potential use of human mineralized waste (feces and urine) as a source of mineral elements for plant cultivation in a biological life support system (BLSS). Plants that are potential candidates for a photosynthesizing link were grown on a neutral solution containing human mineralized waste. Spring wheat Triticum aestivum L., peas Pisum sativum L. Ambrosia cultivar and leaf lettuce Lactuca sativa L., Vitaminny variety, were used. The plants were grown hydroponically on expanded clay aggregates in a vegetation chamber in constant environmental conditions. During plant growth, a determined amount of human mineralized waste was added daily to the nutrient solution. The nutrient solution remained unchanged throughout the vegetation period. Estimated plant requirements for macro-elements were based on a total biological productivity of 0.04 kg day-1 m-2. As the plant requirements for potassium exceeded the potassium content of human waste, a water extract of wheat straw containing the required amount of potassium was added to the nutrient solution. The Knop's solution was used in the control experiments. The experimental and control plants showed no significant differences in state or productivity of their photosynthetic apparatus. A small decrease in total productivity of the experimental plants was observed, which might result in some reduction of O{cyrillic}2 production in a BLSS. В© 2009 COSPAR.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Universite Blaise-Pascal, LGCB, Polytech, BP206, 63174 Aubiere cedex, France
TEC-MCT, ESA/Estec, 1 Keplerlaan, 2201 AZ Noordwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.; Tikhomirov, A.; Shikhov, V.; Kudenko, Yu.; Anischenko, O.; Gros, J.-B.; Lasseur, Ch.

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Production characteristics of the "higher plants-soil-like substrate" system as an element of the bioregenerative life support system / V. V. Velichko [et al.] // Advances in Space Research. - 2013. - Vol. 51, Is. 1. - P115-123, DOI 10.1016/j.asr.2012.08.003 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biological-technical life support system -- Soil-like substrate -- Utilization of plant wastes -- Age groups -- Bioregenerative life support systems -- Cultivated crops -- Cyperus esculentus -- Growth chamber -- Higher plants -- Mineral element -- Nitrogen content -- Nutrient solution -- Plant wastes -- Raphanus sativus -- Root zone -- Soil-like substrate -- Study materials -- Biomass -- Carbon dioxide -- Harvesting -- Minerals -- Plants (botany) -- Soils -- Substrates -- Waste utilization -- Ecology
Аннотация: The study addresses the possibility of long-duration operation of a higher plant conveyor, using a soil-like substrate (SLS) as the root zone. Chufa (Cyperus esculentus L.), radish (Raphanus sativus L.), and lettuce (Lactuca sativa L.) were used as study material. A chufa community consisting of 4 age groups and radish and lettuce communities consisting of 2 age groups were irrigated with a nutrient solution, which contained mineral elements extracted from the SLS. After each harvest, inedible biomass of the harvested plants and inedible biomasses of wheat and saltwort were added to the SLS. The amounts of the inedible biomasses of wheat and saltwort to be added to the SLS were determined based on the nitrogen content of the edible mass of harvested plants. CO2 concentration in the growth chamber was maintained within the range of 1100-1700 ppm. The results of the study show that higher plants can be grown quite successfully using the proposed process of plant waste utilization in the SLS. The addition of chufa inedible biomass to the SLS resulted in species-specific inhibition of growth of both cultivated crops and microorganisms in the "higher plants - SLS" system. There were certain differences between the amounts of some mineral elements removed from the SLS with the harvested edible biomass and those added to it with the inedible biomasses of wheat and saltwort. В© 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Science, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Velichko, V.V.; Tikhomirov, A.A.; Ushakova, S.A.; Tikhomirova, N.A.; Shihov, V.N.; Tirranen, L.S.; Gribovskaya, I.A.

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A biological method of including mineralized human liquid and solid wastes into the mass exchange of bio-technical life support systems / S. A. Ushakova [et al.] // Advances in Space Research. - 2012. - Vol. 50, Is. 7. - P932-940, DOI 10.1016/j.asr.2012.05.023 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bio-technical life support system -- Conveyor-grown uneven-aged communities of wheat and Salicornia -- Utilization of human wastes -- Age groups -- Biological methods -- Dry weight -- Expanded clay -- Harvest index -- Human waste -- Leafy vegetables -- Liquid wastes -- Mass exchange -- Mineral element -- Nutrient solution -- Plant communities -- Salicornia europaea -- Salt content -- Time interval -- Uneven-aged -- Water culture -- Wheat biomass -- Conveyors -- Incineration -- Irrigation -- Minerals -- Nutrients -- Plants (botany) -- Productivity -- Sodium chloride -- Waste incineration -- Waste utilization -- Liquids
Аннотация: The main obstacle to using mineralized human solid and liquid wastes as a source of mineral elements for plants cultivated in bio-technical life support systems (BLSS) is that they contain NaCl. The purpose of this study is to determine whether mineralized human wastes can be used to prepare the nutrient solution for long-duration conveyor cultivation of uneven-aged wheat and Salicornia europaea L. plant community. Human solid and liquid wastes were mineralized by the method of "wet incineration" developed by Yu. Kudenko. They served as a basis for preparing the solutions that were used for conveyor-type cultivation of wheat community represented by 5 age groups, planted with a time interval of 14 days. Wheat was cultivated hydroponically on expanded clay particles. To reduce salt content of the nutrient solution, every two weeks, after wheat was harvested, 12 L of solution was removed from the wheat irrigation tank and used for Salicornia europaea cultivation in water culture in a conveyor mode. The Salicornia community was represented by 2 age groups, planted with a time interval of 14 days. As some portion of the nutrient solution used for wheat cultivation was regularly removed, sodium concentration in the wheat irrigation solution did not exceed 400 mg/L, and mineral elements contained in the removed portion were used for Salicornia cultivation. The experiment lasted 4 months. The total wheat biomass productivity averaged 30.1 gВ·m -2В·day -1, and the harvest index amounted to 36.8%. The average productivity of Salicornia edible biomass on a dry weight basis was 39.3 gВ·m -2В·day -1, and its aboveground mass contained at least 20% of NaCl. Thus, the proposed technology of cultivation of wheat and halophyte plant community enables using mineralized human wastes as a basis for preparing nutrient solutions and including NaCl in the mass exchange of the BLSS; moreover, humans are supplied with additional amounts of leafy vegetables. В© 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian State Technological University, 82, Mir Avenue, Krasnoyarsk 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Tikhomirov, A.A.; Tikhomirova, N.A.; Kudenko, Y.A.; Litovka, Y.A.; Anishchenko, O.V.

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Influence of high concentrations of mineral salts on production process and NaCl accumulation by Salicornia europaea plants as a constituent of the LSS phototroph link / N. A. Tikhomirova [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1589-1593, DOI 10.1016/j.asr.2005.01.055 . - ISSN 0273-1177
Кл.слова (ненормированные):
BLSS -- Intensity of PAR -- Mineral nutrition -- Productivity -- Salicornia europaea -- Biomass -- Concentration (process) -- Nitrogen -- Nutrition -- Photosynthesis -- Productivity -- Sodium chloride -- Bioregenerative life support systems (BLSS) -- Intensity of PAR -- Mineral nutrition -- Salicornia europaea -- Plants (botany) -- calcium -- magnesium -- nitrogen -- phosphorus -- potassium -- sodium chloride -- sulfur -- urea -- biomass -- conference paper -- culture medium -- dose response -- drug effect -- goosefoot -- growth, development and aging -- human -- light -- metabolism -- microclimate -- radiation exposure -- urine -- Biomass -- Calcium -- Chenopodiaceae -- Culture Media -- Dose-Response Relationship, Drug -- Ecological Systems, Closed -- Humans -- Life Support Systems -- Light -- Magnesium -- Nitrogen -- Phosphorus -- Potassium -- Sodium Chloride -- Sulfur -- Urea -- Urine
Аннотация: Use of halophytes (salt-tolerant vegetation), in a particular vegetable Salicornia europaea plants which are capable of utilizing NaCl in rather high concentrations, is one of possible means of NaCl incorporation into mass exchange of bioregenerative life support systems. In preliminary experiments it was shown that S. europaea plants, basically, could grow on urine pretreated with physicochemical processing and urease-enzyme decomposing of urea with the subsequent ammonia distillation. But at the same time inhibition of the growth process of the plants was observed. The purpose of the given work was to find out the influence of excessive quantities of some mineral elements contained in products of physicochemical processing of urine on the production process and NaCl accumulation by S. europaea plants. As the content of mineral salts in the human liquid wastes (urine) changed within certain limits, two variants of experimental solutions were examined. In the first variant, the concentration of mineral salts was equivalent to the minimum salt content in the urine and was: K - 1.5 g/l, P - 0.5 g/l, S - 0.5 g/l, Mg - 0.07 g/l, Ca - 0.2 g/l. In the second experimental variant, the content of mineral salts corresponded to the maximum salt content in urine and was the following: K - 3.0 g/l, P - 0.7 g/l, S - 1.2 g/l, Mg - 0.2 g/l, Ca - 0.97 g/l. As the control, the Tokarev nutrient solution containing nitrogen in the form of a urea, and the Knop nutrient solution with nitrogen in the nitrate form were used. N quantity in all four variants made up 177 mg/l. Air temperature was 24 В°C, illumination was continuous. Light intensity was 690 ?mol/m2s of photosynthetically active radiation. NaCl concentration in solutions was 1%. Our researches showed that the dry aboveground biomass of an average plant of the first variant practically did not differ from the control and totaled 11 g. In the second variant, S. europaea productivity decreased and the dry aboveground biomass of an average plant totaled 8 g. The increase of K quantity in the experimental solutions resulted in an elevated content of the element in the plants. The increase of K uptake in the second experimental variant was accompanied by a 30-50% decrease of Na content in comparison with the other variants. Comparative Na content in the other variants was practically identical. N, Mg and P content in the control and experimental variants was also practically identical. The increase of S quantity in the second experimental variant also increased S uptake by the plants. But Ca quantity, accumulated in aboveground plants biomass in the experimental variants was lower than in the control. NaCl uptake by plants, depending on the concentration of mineral salts in the experimental solutions, ranged from 8 g (maximum salt content) up to 15 g (minimum salt content) on a plant growth area that totaled 0.032 m2. Thus, high concentrations of mineral salts simulating the content of mineral salts contained in urine did not result in a significant decrease of S. europaea productivity. The present work also considers the influence of higher light intensity concentrations on productivity and NaCl accumulation by S. europaea plants grown on experimental solutions with high salt content. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirova, N.A.; Ushakova, S.A.; Kovaleva, N.P.; Gribovskaya, I.V.; Tikhomirov, A.A.

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Direct utilization of human liquid wastes by plants in a closed ecosystem / G. M. Lisovsky [et al.] // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1801-1804 . - ISSN 0273-1177
Кл.слова (ненормированные):
fertilizer -- nitrogen -- sodium chloride -- urea -- article -- biomass -- culture medium -- feasibility study -- human -- metabolism -- methodology -- microclimate -- sewage -- urine -- waste management -- wheat -- Biomass -- Culture Media -- Ecological Systems, Closed -- Feasibility Studies -- Fertilizers -- Humans -- Nitrogen -- Sodium Chloride -- Triticum -- Urea -- Urine -- Waste Disposal, Fluid -- Waste Management
Аннотация: Model experiments in phytotrons have shown that urea is able to cover 70% of the demand in nitrogen of the conveyer cultivated wheat. At the same time wheat plants can directly utilize human liquid wastes. In this article by human liquid wastes the authors mean human urine only. In a long-term experiment on "man-higher plants" system with two crewmen, plants covered 63 m2, with wheat planted to - 39.6 m2. For 103 days, complete human urine (total amount - 210.7 1) wassupplied into the nutrient solution for wheat. In a month and a half NaCl supply into the nutrient solution stabilized at 0.9-1.65 g/l. This salination had no marked effect on wheat production. The experiment revealed the realistic feasibility to directly involve liquid wastes into the biological turnover of the life support system. The closure of the system, in terms of water, increased by 15.7% and the supply of nutrients for wheat plants into the system was decreased. Closedness of biological turnover of matter in a man-made "man - higher plants" ecological system might involve, among other processes, direct utilization of human liquid wastes by plants. The amount of urine comprises 15-20% of the total amount of water cycling within the system including water as part of food, household, hygiene and potable water necessary for man. What is more, it they contains most nitrogen-bearing compounds emitted by man, almost all of the NaCl and some other substances involved in the biological turnover. Human liquid wastes can be utilized either by preliminary physical-chemical treatment (evaporating or freezing out the water, finally oxidizing the organic matter, isolating the mineral components required for plants, etc.) and further involvement of the obtained products or by direct application into the nutrient solution for plants. The challenge of direct utilization is that plants have no need of Na+ and Cl-, and also the organic forms of nitrogen emitted by man cannot fully meet the demand of plants forthis element. Besides, hygienic and/or psychological reasons make it desirable to avoid direct use of liquid wastes in the nutrient solutions that would have direct contact with edible part of plants (tubers, roots, bulbs). Feasibility of direct utilization of liquid wastes by plants in a closed "man - higher plants" ecosystem has been experimentally studied on wheat - grain culture as a model plant with the edible part in the form of seeds spatially dissociated with the nutrient medium. The wheat covered 60-65% of the area under higher plants. The studies have been carried out in "Bios-3"experimental facility described in detail elsewhere (Lisovsky, 1979; Gitelson et al., 1989). В© 1997 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Inst. Biophys.-Russ. Acad. of Sci., Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lisovsky, G.M.; Gitelson, J.I.; Shilenko, M.P.; Gribovskaya, I.V.; Trubachev, I.N.

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10.

Plants-rhizospheric organisms interaction in a manmade system with and without biogenous element limitation / L. A. Somova [et al.] // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1939-1943 . - ISSN 0273-1177
Кл.слова (ненормированные):
nitrogen -- article -- bacterial count -- biomass -- comparative study -- culture medium -- growth, development and aging -- metabolism -- microbiology -- plant leaf -- plant root -- plant seed -- Pseudomonas fluorescens -- Pseudomonas putida -- wheat -- Biomass -- Colony Count, Microbial -- Culture Media -- Nitrogen -- Plant Leaves -- Plant Roots -- Pseudomonas fluorescens -- Pseudomonas putida -- Seeds -- Triticum
Аннотация: The effect has been studied of inoculation of seeds of wheat with two species of rhizospheric microorganisms, -Pseudomonas fluorescens and Pseudomonas putida - on young plant growth with complete and with nitrogen deficit mineral nutrition. With complete mineral medium, plants grown from seeds inoculated with bacteria of Pseudomonas genus (experiment plants) have been found to have better growth over plants not inoculated with these bacteria (control plants). The experiment plants had increased transpiration and their biomass had higher organic nitrogen content. With nitrogen deficit medium, the plants inoculated with bacteria and those without them, have not revealed changes in growth. Neither case demonstrated competition of microorganisms with plants for nitrogen sources. В© 1997 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk 6600036, Russian Federation
CEA-Sciences du Vivant, DEVM, CEA/Cadarache, Saint-Paul-Lez-Durance Cedex, France
Computing Center Russian, Krasnoyarsk 6600036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Polonsky, V.I.; Pisman, T.I.; Sarangova, A.B.; Andre, M.; Sadovskaya, G.M.

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11.

Numerical modeling of the hydrophysical influence effects on the phytoplankton distribution / V. Kartushinsky Alexei // Mathematical Biology and Bioinformatics. - 2012. - Vol. 7, Is. 1. - С. 112-124 . - ISSN 1994-6538
Кл.слова (ненормированные):
Aquatic ecosystem -- Biogenic element -- Chlorophyll -- Hydrophysical processes -- Inhomogeneity -- Numerical model -- Phytoplankton -- Rainfall -- Turbulent diffusion -- Upwelling -- Variability
Аннотация: The paper describes simulating results of the phytoplankton vertical distribution in the aquatic ecosystems based on one-dimensional numerical model. The model is used to calculate the dynamics of the temperature, salt, limiting nutrient and phytoplankton biomass. The work shows some temporal variability of phytoplankton distribution in the surface layer of salt and freshwater lake ecosystems under the impact of hydrophysical processes. These results can be used for the satellite date interpretation.

Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kartushinsky Alexei, V.

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12.

Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters [Text] / L. A. Frank // Sensors. - 2010. - Vol. 10, Is. 12. - P11287-11300, DOI 10.3390/s101211287. - Cited References: 70. - This work was supported by the grant No. 76 of the Russian Academy of Sciences, Siberian Branch. . - ISSN 1424-8220

РУБ Chemistry, Analytical + Electrochemistry + Instruments & Instrumentation
Рубрики:
POLYMERASE-CHAIN-REACTION
   CYTOKINE MESSENGER-RNA
   BIOLUMINESCENT IMMUNOASSAY
   MYCOBACTERIUM-TUBERCULOSIS
   BIOTINYLATED AEQUORIN
   RECOMBINANT AEQUORIN
   ANGSTROM RESOLUTION
   FUSION PROTEIN
   PCR ASSAY
   OBELIN
Кл.слова (ненормированные):
bioluminescence -- Ca2+-regulated photoprotein -- immunoassay -- PCR-ELISA -- multiplex assay -- re-engineered photoproteins
Аннотация: Ca2+-regulated photoproteins of luminous marine coelenterates are of interest and a challenge for researchers as a unique bioluminescent system and as a promising analytical instrument for both in vivo and in vitro applications. The proteins are comprehensively studied as to biochemical properties, tertiary structures, bioluminescence mechanism, etc. This knowledge, along with available recombinant proteins serves the basis for development of unique bioluminescent detection systems that are "self-contained", triggerable, fast, highly sensitive, and non-hazardous. In the paper, we focus on the use of photoproteins as reporters in binding assays based on immunological recognition element-bioluminescent immunoassay and hybridization immunoassay, their advantages and prospects.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.

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13.

Theoretical analysis of optical spectra of algal absorption / L. E. Paramonov // Oceanology. - 1996. - Vol. 35, Is. 5. - P. 655-659 . - ISSN 0001-4370
Аннотация: A formula for the absorption cross section of macroisotropic volume element containing soft particles is proposed. A formula is given that describes transformation of the absorption cross section with granulometric composition of a substance. Absorption spectra for the green, blue-green, and diatom algae are examined against microstructure of suspension. Some problems of inverse task solutions are discussed.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Paramonov, L.E.

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14.

LIGHT-SCATTERING BY RANDOMLY ORIENTED PARTICLES INTO SOLID ANGLES [Text] / L. E. PARAMONOV // J. Opt. Soc. Am. A-Opt. Image Sci. Vis. - 1994. - Vol. 11, Is. 4. - P. 1360-1369, DOI 10.1364/JOSAA.11.001360. - Cited References: 38 . - ISSN 0740-3232

РУБ Optics
Рубрики:
NONSPHERICAL PARTICLES
POLARIZED-LIGHT
Аннотация: Light scattering by a macroscopically isotropic volume element consisting of randomly oriented particles is considered. The expansion of scattering-matrix elements in a series of generalized spherical functions is used to develop a rigorous analytical method for computing scattered flux into any solid angle for incoherent parallel, convergent, or divergent incident beams. Some illustrative numerical results are reported.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
PARAMONOV, L.E.

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15.

T-MATRIX APPROACH AND THE ANGULAR-MOMENTUM THEORY IN LIGHT-SCATTERING PROBLEMS BY ENSEMBLES OF ARBITRARILY-SHAPED PARTICLES [Text] / L. E. PARAMONOV // J. Opt. Soc. Am. A-Opt. Image Sci. Vis. - 1995. - Vol. 12, Is. 12. - P. 2698-2707, DOI 10.1364/JOSAA.12.002698. - Cited References: 38 . - ISSN 0740-3232

РУБ Optics
Рубрики:
ELECTROMAGNETIC SCATTERING
NONSPHERICAL GRAINS
EXTINCTION
Аннотация: Light scattering by a macroscopically anisotropic volume element, which consists of arbitrarily shaped particles with an arbitrary square-integrable probability-density function over orientations, is considered. The T-matrix approach and the quantum theory of angular momentum are used to develop a rigorous analytical method (in terms of the T matrix) for computing the extinction matrix; the extinction, scattering, and absorption cross sections; and the elements of the Mueller matrix of the volume element. The constructive theorem of the existence of the expansion of scattering-matrix elements in a series of Wigner functions for a medium with rotation symmetry is proved. The analytical results are generalized for incident radiation composed of incoherent plane waves with a square-integrable probability-density function over directions of propagation, intensities, and polarization states. (C) 1995 Optical Society of America.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
PARAMONOV, L.E.

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16.

A THEORETICAL-ANALYSIS OF OPTICAL-ABSORPTION SPECTRA OF ALGAE [Текст] / L. E. PARAMONOV // Okeanologiya. - 1995. - Vol. 35, Is. 5. - P. 719-724. - Cited References: 30 . - ISSN 0030-1574

РУБ Oceanography
Рубрики:
NATURAL-WATERS
   PHYTOPLANKTON PIGMENTS
   LIGHT
   ATTENUATION
   CELLS
   CHLOROPHYLL
   SCATTERING
   COASTAL
Аннотация: The formula for the absorption cross sections of macroscopically isotropic volume element which consists of ''soft'' particles is proposed. The transformation formula for absorption cross sections on disperse composition is given. The dependence of absorption spectra of green, blue-green and diatom algae on microstructure of suspension is considered. Some inverse problems are discussed.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
PARAMONOV, L.E.

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17.

Bioluminescent detection probe for tick-borne encephalitis virus immunoassay [Text] / L. P. Burakova [et al.] // Anal. Bioanal. Chem. - 2015. - Vol. 407, Is. 18. - P5417-5423, DOI 10.1007/s00216-015-8710-6. - Cited References:19. - The work was supported by the Russian Academy of Sciences, Siberian Branch, within the framework of the Interdisciplinary Integration Project No. 139 and the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No. VI 57.1.1). . - ISSN 1618-2642. - ISSN 1618-2650

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
COELENTERAZINE-BINDING PROTEIN
ENZYME-IMMUNOASSAY
RENILLA-MUELLERI
Кл.слова (ненормированные):
Tick-borne encephalitis virus -- Single-chain antibody -- Luciferase -- Immunoassay
Аннотация: To facilitate the detection of the tick-borne encephalitis virus (TBEV), the causative agent of one of the most severe human neuroinfections, we have developed an immunoassay based on bioluminescent hybrid protein 14D5a-Rm7 as a detection probe. The protein containing Renilla luciferase as a reporter and a single-chain variable fragment (scFv) of murine immunoglobulin to TBEV as a recognition element was constructed, produced by bacterial expression, purified, and tested. Both domains were shown to reveal their specific biological properties-affinity to the target antigen and bioluminescent activity. Hybrid protein was applied as a label for solid-phase immunoassay of the antigens, associated with the tick-borne encephalitis virus (native glycoprotein E or extracts of the infected strain of lab ticks). The assay demonstrates high sensitivity (0.056 ng of glycoprotein E; 10(4)-10(5) virus particles or 0.1 pg virions) and simplicity and is competitive with conventional methods for detection of TBEV.

WOS,
Scopus
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Branch, Novosibirsk 630090, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Inst Poliomyelitis & Viral Encephalitides, Moscow 142782, Russia.
Res Inst Nat Foci Infect, Omsk 644080, Russia.

Доп.точки доступа:
Burakova, Ludmila P.; Kudryavtsev, Alexander N.; Stepanyuk, Galina A.; Baykov, Ivan K.; Morozova, Vera V.; Tikunova, Nina V.; Dubova, Maria A.; Lyapustin, Victor N.; Yakimenko, Valeri V.; Frank, Ludmila A.; Russian Academy of Sciences, Siberian Branch [139]; Russian Academy of Sciences [VI 57.1.1]

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18.

Benefit-risk ratio of canned pacific saury (Cololabis saira) intake: Essential fatty acids vs. heavy metals / O. V. Anishchenko [et al.] // Food Chem. Toxicol. - 2017. - Vol. 101. - P8-14, DOI 10.1016/j.fct.2016.12.035 . - ISSN 0278-6915
Кл.слова (ненормированные):
Benefit-risk ratio -- Canned fish -- Heavy metals -- Polyunsaturated fatty acids (PUFA)
Аннотация: Fatty acid (FA) and element contents were studied in 14 brands of canned (in its own juice and with sunflower oil) saury (Cololabis saira), a popular product of Russian market. Canned saury is a valuable source of essential polyunsaturated FA - eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). To obtain personal daily dose of EPA + DHA of 1 g for prevention of cardiovascular diseases, neural and inflammatory disorders one needs to intake from 26 to 76 g of canned saury, as was calculated for studied brands. ICP-OES analysis of 24 elements showed that Pb concentration in one brand and Cd content in most of studied saury samples exceeded standards for fish meat established by the European Commission. However, values of hazard quotient, HQEFA which estimate benefit-risk ratio of fish intake, indicate that canned saury is safe product for human nutrition. © 2016 Elsevier Ltd

Scopus,
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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok, 50/50, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodny av., Krasnoyarsk, Russian Federation
Federal Regional Center for Standardization, Metrology and Testing in the Krasnoyarsk Region, 1a Vavilov Str., Krasnoyarsk, Russian Federation

Доп.точки доступа:
Anishchenko, O. V.; Sushchik, N. N.; Makhutova, O. N.; Kalachova, G. S.; Gribovskaya, I. V.; Morgun, V. N.; Gladyshev, M. I.

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19.

Benefit-risk ratio of canned pacific saury (Cololabis saira) intake: Essential fatty acids vs. heavy metals / O. V. Anishchenko [et al.] // Food Chem. Toxicol. - 2017. - Vol. 101. - P8-14, DOI 10.1016/j.fct2016.12.035. - Cited References:51. - The work was partly supported by grant NSh-9249.2016.5 from the President of the Russian Federation. . - ISSN 0278-6915. - ISSN 1873-6351

РУБ Food Science & Technology + Toxicology
Рубрики:
EASTERN MEDITERRANEAN SEA
BREAM SPARUS-AURATA
ESSENTIAL ELEMENTS
Кл.слова (ненормированные):
Polyunsaturated fatty acids (PUFA) -- Benefit-risk ratio -- Heavy metals -- Canned fish
Аннотация: Fatty acid (FA) and element contents were studied in 14 brands of canned (in its own juice and with sunflower oil) saury (Cololabis saira), a popular product of Russian market. Canned saury is a valuable source of essential polyunsaturated FA - eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). To obtain personal daily dose of EPA + DHA of 1 g for prevention of cardiovascular diseases, neural and inflammatory disorders one needs to intake from 26 to 76 g of canned saury, as was calculated for studied brands. ICP-OES analysis of 24 elements showed that Pb concentration in one brand and Cd content in most of studied saury samples exceeded standards for fish meat established by the European Commission. However, values of hazard quotient, HQEFA, which estimate benefit -risk ratio of fish intake, indicate that canned saury is safe product for human nutrition. (C) 2016 Elsevier Ltd. All rights reserved.

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Смотреть статью
Держатели документа:
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Av, Krasnoyarsk 660041, Russia.
Fed Reg Ctr Standardizat Metrol & Testing Krasnoy, 1a Vavilov Str, Krasnoyarsk 660093, Russia.

Доп.точки доступа:
Anishchenko, O. V.; Sushchik, N. N.; Makhutova, O. N.; Kalachova, G. S.; Gribovskaya, I. V.; Morgun, V. N.; Gladyshev, M. I.; Russian Federation [NSh-9249.2016.5]

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20.

Developing of discrimination experiment to find most adequate model of plant's multi-nutrient functional response / M. Yu Saltykov, S. I. Bartsev // IOP Conference Series: Materials Science and Engineering : Institute of Physics Publishing, 2017. - Vol. 173: 5th International Workshop on Mathematical Models and their Applications 2016, IWMMA 2016 (7 November 2016 through 9 November 2016, ) Conference code: 126825, Is. 1, DOI 10.1088/1757-899X/173/1/012017
Кл.слова (ненормированные):
Ecology -- Metabolism -- Physiology -- Adequate models -- Complex model -- Discrimination experiments -- Ecological models -- Functional response -- Trophic level -- Typical patterns -- Nutrients
Аннотация: To create reliable Closed Ecological Life Support Systems (CELSS) it is necessary to have models which can predict CELSS dynamic with good accuracy. However it was shown that conventional ecological models cannot describe CELSS correctly if it is closed by more than one element. This problem can be solved by means more complex models than conventional ones - so called flexible metabolism models. However it is possible that CELSS also can be described correctly in "semi-conventional" framework - when only one trophic level is described by flexible metabolism model. Another problem in CELSS modeling is existence of different and incompatible hypotheses about relationships between plants growth rate and amounts of nutrients (functional responses). Difficulty of testing these hypotheses is associated with multi-nutrient dependency of growth rate and comprehensive experimental studies are expensive and time-consuming. This work is devoted to testing the hypothesis that "semi-conventional" approach is enough to describe CELSS, and to planning the discrimination experiment on selecting correct type of the plant's functional response. To do that three different models of plants (one flexible and two conventional) were investigated both in the scope of CELSS model, and in hemostat model. Numerical simulations show that each of the models has typical patterns which can be determined in experiment with real plants. © Published under licence by IOP Publishing Ltd.

Scopus,
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WOS
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok 50-50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Yu Saltykov, M.; Bartsev, S. I.

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