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Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562 / T. G. Volova [et al.] // Applied Microbiology and Biotechnology. - 2006. - Vol. 73, Is. 3. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Biochemistry -- Carbon -- Genetic engineering -- Glucose -- Hydrogen -- Polyesters -- Cultural properties -- Glucose-utilizing mutant strain -- Nitrogen deficiency -- Polyhydroxyalkanoate (PHA) -- Bacteria -- 3 hydroxybutyric acid -- carbon dioxide -- fructose -- glucose -- hydrogen -- monomer -- polyhydroxyalkanoic acid -- sugar -- bacterium -- carbon -- comparative study -- energy -- ester -- experimental study -- glucose -- hydrogen -- physicochemical property -- article -- bacterial strain -- bacterium culture -- bacterium mutant -- biomass -- chemical analysis -- Cupriavidus necator -- energy consumption -- genetic analysis -- nonhuman -- synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO2, fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is ?-hydroxybutyric acid (more than 99 mol %); the identified minor components are ?-hydroxyvaleric acid (0.25-0.72 mol %) and ?-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose. В© Springer-Verlag 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. / T. G. Volova [et al.] // Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
beta hydroxyvaleric acid -- beta-hydroxyvaleric acid -- carbon dioxide -- ester -- fatty acid -- fructose -- glucose -- hexanoic acid derivative -- hydrogen -- hydroxyacid -- oxygen -- polymer -- ribosome RNA -- valeric acid derivative -- article -- biotechnology -- chemistry -- metabolism -- methodology -- Wautersia eutropha -- Biotechnology -- Carbon Dioxide -- Cupriavidus necator -- Esters -- Fatty Acids -- Fructose -- Glucose -- Hexanoic Acids -- Hydrogen -- Hydroxy Acids -- Oxygen -- Pentanoic Acids -- Polymers -- RNA, Ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69 . - ISSN 0026-3656
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Cupriavidus necator -- acetoacetyl coenzyme a -- acetyl coenzyme A acyltransferase -- acyl coenzyme A -- acyltransferase -- butyric acid derivative -- carbon dioxide -- fructose -- hydrogen -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3-hydroxyalkanoic acid) synthase -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- polyester -- polyhydroxyalkanoate synthase -- valeric acid derivative -- article -- chemistry -- crystallization -- culture medium -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyl Coenzyme A -- Acyltransferases -- Butyrates -- Carbon Dioxide -- Crystallization -- Culture Media -- Cupriavidus necator -- Fructose -- Hydrogen -- Polyesters -- Valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562 / T. G. Volova [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 6. - С. 788-794 . - ISSN 0026-3656
Кл.слова (ненормированные):
Glucose-utilizing strain -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates -- Synthesis -- Bacteria (microorganisms) -- Cupriavidus necator -- bacterial RNA -- glucose -- hydrogen -- hydroxybutyric acid -- polymer -- RNA 16S -- article -- culture medium -- genetics -- growth, development and aging -- metabolism -- mutation -- oxidation reduction reaction -- physiology -- Wautersia eutropha -- Culture Media -- Cupriavidus necator -- Glucose -- Hydrogen -- Hydroxybutyrates -- Mutation -- Oxidation-Reduction -- Polymers -- RNA, Bacterial -- RNA, Ribosomal, 16S
Аннотация: The physiological, biochemical, genetic, and cultural characteristics of the glucose-utilizing mutant strain Ralstonia eutropha B8562 were investigated in comparison with the parent strain R. eutropha B5786. The morphological, cultural, and biochemical characteristics of strain R. eutropha B8562 were similar to those of strain R. eutropha B5786. Genetic analysis revealed differences between the 16S rRNA gene sequences of these strains. The growth characteristics of the mutant using glucose as the sole carbon and energy source were comparable with those of the parent strain grown on fructose. Strain B8562 was characterized by high yields of polyhydroxyalkanoate (PHA) from different carbon sources (CO 2, fructose, and glucose). In batch culture with glucose under nitrogen limitation, PHA accumulation reached 90% of dry weight. In PHA, ?-hydroxybutyrate was predominant (over 99 mol %); ?-hydroxyvalerate (0.25-0.72 mol %) and ?-hydroxyhexanoate (0.008-1.5 mol %) were present as minor components. The strain has prospects as a PHA producer on glucose-containing media.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kozhevnikov, I.V.; Dolgopolova, Yu.B.; Trusova, M.Yu.; Kalacheva, G.S.; Aref'eva, Yu.V.

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Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562 / T. G. Volova [et al.] // Microbiology. - 2005. - Vol. 74, Is. 6. - P684-689, DOI 10.1007/s11021-005-0124-6 . - ISSN 0026-2617
Кл.слова (ненормированные):
Glucose-utilizing strain -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates -- Synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: The physiological, biochemical, genetic, and cultural characteristics of the glucose-utilizing mutant strain Ralstonia eutropha B8562 were investigated in comparison with the parent strain R. eutropha B5786. The morphological, cultural, and biochemical characteristics of strain R. eutropha B8562 were similar to those of strain R. eutropha B5786. Genetic analysis revealed differences between the 16S rRNA gene sequences of these strains. The growth characteristics of the mutant using glucose as the sole carbon and energy source were comparable with those of the parent strain grown on fructose. Strain B8562 was characterized by high yields of polyhydroxyalkanoate (PHA) from different carbon sources (CO2, fructose, and glucose). In batch culture with glucose under nitrogen limitation, PHA accumulation reached 90% of dry weight. In PHA, ?-hydroxybutyrate was predominant (over 99 mol %); ?-hydroxyvalerate (0.25-0.72 mol %) and ?-hydroxyhexanoate (0.008-1.5 mol %) were present as minor components. The strain has prospects as a PHA producer on glucose-containing media. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kozhevnikov, I.V.; Dolgopolova, Yu.B.; Trusova, M.Yu.; Kalacheva, G.S.; Aref'eva, Yu.V.

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The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Microbiology. - 2005. - Vol. 74, Is. 1. - P54-59, DOI 10.1007/s11021-005-0028-5 . - ISSN 0026-2617
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass; the yield, synthesis rate, and composition of copolymers; the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis ?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase); the maximum tolerable concentration of valerate to the bacterium; and the conditions that govern the incorporation of hydroxyvalerate into copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of such copolymers depend on the molar fraction of 3HV. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786 / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 2. - P170-177, DOI 10.1023/B:ABIM.0000018921.04863.d5 . - ISSN 0003-6838
Кл.слова (ненормированные):
acetyl coenzyme A acyltransferase -- bacterial enzyme -- carbon -- carbon dioxide -- fructose -- hydrogen -- hydroxybutyrate dehydrogenase -- oxidoreductase -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- synthetase -- article -- bacterial metabolism -- carbon source -- catalysis -- controlled study -- degradation -- depolymerization -- enzyme activity -- enzyme analysis -- molecular dynamics -- nonhuman -- protein function -- Ralstonia eutropha -- recording -- statistical significance -- synthesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (?-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of ?-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha / T. G. Volova [и др.] // Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 2. - С. 201-209 . - ISSN 0555-1099
Кл.слова (ненормированные):
acetoacetyl coenzyme a reductase -- acetoacetyl-CoA reductase -- acetyl coenzyme A acyltransferase -- acyltransferase -- alcohol dehydrogenase -- carboxylesterase -- hydroxybutyrate dehydrogenase -- hydroxybutyric acid -- poly(3 hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) synthase -- polyhydroxyalkanoate synthase -- polymer -- article -- chemistry -- comparative study -- culture medium -- enzymology -- growth, development and aging -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyltransferases -- Alcohol Oxidoreductases -- Carboxylic Ester Hydrolases -- Culture Media -- Cupriavidus necator -- Hydroxybutyrate Dehydrogenase -- Hydroxybutyrates -- Polymers
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of the key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, D-hydroxybutyrate dehydrogenase, and PHA depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase were recorded at the stage of acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only at stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not cause any marked changes in the time course of enzyme activity.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786 [Text] / T. G. Volova [et al.] // Appl. Biochem. Microbiol. - 2004. - Vol. 40, Is. 2. - P. 170-177, DOI 10.1023/B:ABIM.0000018921.04863.d5. - Cited References: 27 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
POLY-BETA-HYDROXYBUTYRATE
   ORGANISM ALCALIGENES-EUTROPHUS
   ESCHERICHIA-COLI
   PHB
   POLY(3-HYDROXYBUTYRATE)
   BIOSYNTHESIS
   CLONING
   GENES
   CHAIN
   H16
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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10.

Fatty acid composition and polyhydroxyalkanoates production by Cupriavidus eutrophus B-10646 cells grown on different carbon sources [Text] / N. Zhila, G. Kalacheva, T. Volova // Process Biochem. - 2015. - Vol. 50, Is. 1. - P69-78, DOI 10.1016/j.procbio.2014.10.018. - Cited References:66. - This study was supported by the Russian Science Foundation (Grant No.14-26-00039). . - ISSN 1359-5113. - ISSN 1873-3298

РУБ Biochemistry & Molecular Biology + Biotechnology & Applied Microbiology
Рубрики:
HYDROGEN-OXIDIZING BACTERIA
CHAIN TRANSFER AGENTS
RALSTONIA-EUTROPHA
Кл.слова (ненормированные):
Cupriavidus eutrophus -- Fatty acid -- Polyhydroxyalkanoates -- Molecular -- weight -- Different substrates
Аннотация: Growth of Cupriavidus eutrophus B-10646, fatty acid (FA) composition of the lipids in the cytoplasmic membrane and cell wall, and polyhydroxyalkanoate (PHA) accumulation by the cells of this new promising producer of PHAs were studied in batch culture, using various carbon sources (fructose, glucose, oleic acid, sunflower seed oil, glycerol, CO2:O-2:H-2). In cells grown on sugars and in autotrophic culture, during the phase of active cell growth, the major FAs of the lipids of cytoplasmic membrane (CMLs) were palmitic, palmitoleic, and cis-vaccenic acids. In the stationary phase of cultivation, cyclopropane FAs were also detected. When carbohydrate substrate was replaced by oleic acid or sunflower seed oil, the proportion of oleic acid in the total FAs of the cytoplasmic membrane increased considerably. In addition to that, the lipid FAs of bacterial cells grown on sunflower seed oil also contained linoleic acid, which is the major acid of sunflower seed oil. The major acids of strongly bound lipids of cell wall (SBLs) were myristic acid and long-chain-length beta-hydroxy acids. Molecular weight of the polymer was measured during its accumulation in cells; for the first time, the presence of 2 polymer fractions (high-molecular weight and low-molecular-weight ones) was recorded in cells with a low polymer content. (C) 2014 Elsevier Ltd. All rights reserved.

WOS
Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Zhila, Natalia; Kalacheva, Galina; Volova, Tatiana; Russian Science Foundation [14-26-00039]

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11.

Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process / T. Volova [et al.] // Biomacromolecules. - 2019, DOI 10.1021/acs.biomac.9b00295 . - Article in press. - ISSN 1525-7797
Кл.слова (ненормированные):
Crystallinity -- Fructose -- Glucose -- Pilot plants -- Purification -- Substrates -- Terpolymers -- Carbon substrates -- Cell concentrations -- Degree of crystallinity -- Hydrogen-oxidizing bacteria -- Polies (3 hydroxybutyrate co 3 hydroxyvalerate co 4 hydroxybutyrate) -- Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) -- Polyhydroxyalkanoates -- Yield coefficient -- Glycerol
Аннотация: The synthesis of polyhydroxyalkanoates (PHAs) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus necator B-10646. Over 60 h of cultivation, a cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in a cell concentration of 130 ± 10 g/L. Polymer content and yield coefficients for the biomass were similar on all substrates (80-85 wt % and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Copolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. necator B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in an organotrophic C. necator B-10646 culture. © 2019 American Chemical Society.

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center Krasnoyarsk Science Center SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Volova, T.; Kiselev, E.; Zhila, N.; Shishatskaya, E.

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12.

Properties of degradable polyhydroxyalkanoates (Phas) synthesized by a new strain, cupriavidus necator ibp/sfu-1, from various carbon sources / N. O. Zhila, K. Yu. Sapozhnikova, E. G. Kiselev [et al.] // Polym. - 2021. - Vol. 13, Is. 18. - Ст. 3142, DOI 10.3390/polym13183142 . - ISSN 2073-4360
Кл.слова (ненормированные):
Cell growth and PHA synthesis -- Cupriavidus necator IBP/SFU-1 -- PHA composition and properties -- Polymer films -- Various carbon sources -- Biodegradable polymers -- Carbon -- Carbon films -- Cell proliferation -- Crystallinity -- Fructose -- Glucose -- Long Term Evolution (LTE) -- Oleic acid -- Organic carbon -- Palm oil -- Polydispersity -- Semiconducting films -- Autotrophics -- Carbon source -- Cell growth and PHA synthesis -- Cupriavidu necator IBP/SFU-1 -- PHA composition and property -- Plant oil -- Polyhydroxyalkanoates -- Property -- Synthesised -- Various carbon source -- Polymer films
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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Держатели документа:
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, L.V. Kirensky Institute of Physics SB RAS, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Zhila, N. O.; Sapozhnikova, K. Yu.; Kiselev, E. G.; Vasiliev, A. D.; Nemtsev, I. V.; Shishatskaya, E. I.; Volova, T. G.

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13.

Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources / N. O. Zhila, K. Y. Sapozhnikova, E. G. Kiselev [et al.] // Polymers. - 2021. - Vol. 13, Is. 18. - Ст. 3142, DOI 10.3390/polym13183142. - Cited References:78. - This work was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 075-15-2021-626) in accordance with Resolution No 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (strain isolation, polymer synthesis and investigation), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (study of film properties). . - ISSN 2073-4360

РУБ Polymer Science
Рубрики:
RALSTONIA-EUTROPHA
   POLY(3-HYDROXYBUTYRATE) PRODUCTION
   PLANT OIL
   ACID
Кл.слова (ненормированные):
Cupriavidus necator IBP -- SFU-1 -- cell growth and PHA synthesis -- various -- carbon sources -- PHA composition and properties -- polymer films
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7-8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 +/- 15 kDa) and weight-average (850 +/- 25 kDa) molecular weights and the lowest polydispersity (2.9 +/- 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.

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Держатели документа:
Siberian Fed Univ, Basic Dept Biotechnol, Sch Fundamental Biol & Biotechnol, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, LV Kirensky Inst Phys, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zhila, Natalia O.; Sapozhnikova, Kristina Yu; Kiselev, Evgeniy G.; Vasiliev, Alexander D.; Nemtsev, Ivan, V; Shishatskaya, Ekaterina, I; Volova, Tatiana G.; Russian FederationRussian Federation [075-15-2021-626, 220]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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14.

Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass / T. G. Volova, E. G. Kiselev, A. V. Demidenko [et al.] // Polymers. - 2022. - Vol. 14, Is. 1. - Ст. 132, DOI 10.3390/polym14010132. - Cited References:93. - This study was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No. 075-15-2021-626) in accordance with Resolution No. 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis, properties), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (films production, surface properties). . - ISSN 2073-4360

РУБ Polymer Science
Рубрики:
GLUCOSE-UTILIZING STRAIN
   RALSTONIA-EUTROPHA
   ASPERGILLUS-NIGER
   ACID
Кл.слова (ненормированные):
Jerusalem artichoke hydrolysates -- PHA synthesis -- productivity -- polyhydroxyalkanoates
Аннотация: One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains-C. necator B-10646 and R. eutropha B 5786 and B 8562-were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 degrees C followed by acid hydrolysis at 60 degrees C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100-120 degrees C difference between the T-melt and T-degr, prevailing of the crystalline phase over the amorphous one (C-x between 69 and 75%), and variations in weight average molecular weight (409-480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and epsilon-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology-finding widely available and inexpensive substrates.

WOS
Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Basic Dept Biotechnol, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys, SB RAS, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, LV Kirensky Phys Inst, Fed Res Ctr, SB RAS, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, Tatiana G.; Kiselev, Evgeniy G.; Demidenko, Alexey V.; Zhila, Natalia O.; Nemtsev, Ivan V.; Lukyanenko, Anna V.; Kiselev, Evgeniy; Project "Agropreparations of the new generation: a strategy of construction and realization" [075-15-2021-626]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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