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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (1)

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On mechanism of antioxidant effect of fullerenols / A. S. Sachkova [et al.] // Biochem. Biophys. Rep. - 2017. - Vol. 9. - P1-8, DOI 10.1016/j.bbrep.2016.10.011 . - ISSN 2405-5808
Кл.слова (ненормированные):
Antioxidant activity -- Bacterial enzymes -- Fullerenol -- Hormesis -- Luminous marine bacteria -- Ultralow concentrations
Аннотация: Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic toxicants of oxidative type – 1,4-benzoquinone and potassium ferricyanide. Two fullerenol preparations were tested: С60О2–4(ОН)20–24 and mixture of two types of fullerenols С60О2–4(ОН)20–24+С70О2–4(ОН)20–24. Bacteria-based and enzyme-based bioluminescent assays were used to evaluate a decrease in cellular and biochemical toxicities, respectively. Additionally, the enzyme-based assay was used for the direct monitoring of efficiency of the oxidative enzymatic processes. The bacteria-based and enzyme-based assays showed similar peculiarities of the detoxification processes: (1) ultralow concentrations of fullerenols were active (ca 10–17–10?4 and 10–17–10? 5 g/L, respectively), (2) no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and (3) detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effect of highly diluted fullerenol solutions on bacterial cells was attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures. Sequence analysis of 16S ribosomal RNA was carried out; it did not reveal mutations in bacterial DNA. The suggestion was made that hydrophobic membrane-dependent processes are involved to the detoxifying mechanism. Catalytic activity of fullerenol (10? 8 g/L) in NADH-dependent enzymatic reactions was demonstrated and supposed to contribute to adaptive bacterial response. © 2016 The Authors

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Держатели документа:
National Research Tomsk Polytechnic University, Tomsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Physics SB RAS, Krasnoyarsk, Russian Federation
SB RAS Genomics Core Facility, Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russian Federation

Доп.точки доступа:
Sachkova, A. S.; Kovel, E. S.; Churilov, G. N.; Guseynov, O. A.; Bondar, A. A.; Dubinina, I. A.; Kudryasheva, N. S.

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Exposure of luminous marine bacteria to low-dose gamma-radiation / N. S. Kudryasheva [et al.] // J. Environ. Radioact. - 2017. - Vol. 169-170. - P64-69, DOI 10.1016/j.jenvrad.2017.01.002 . - ISSN 0265-931X
Кл.слова (ненормированные):
Bioassay -- Low-dose gamma-radiation -- Luminous marine bacteria -- Mutagenic effect -- Radiotoxicity -- Temperature dependence -- Bacteria -- Bioassay -- Bioluminescence -- Gamma rays -- Ionizing radiation -- Irradiation -- Phosphorescence -- Physiological models -- Radiation effects -- Temperature distribution -- Low dose -- Marine bacterium -- Mutagenic effect -- Radiotoxicity -- Temperature dependence -- Radiation -- Bacteria (microorganisms) -- Photobacterium phosphoreum
Аннотация: The study addresses biological effects of low-dose gamma-radiation. Radioactive 137Cs-containing particles were used as model sources of gamma-radiation. Luminous marine bacterium Photobacterium phosphoreum was used as a bioassay with the bioluminescent intensity as the physiological parameter tested. To investigate the sensitivity of the bacteria to the low-dose gamma-radiation exposure (?250 mGy), the irradiation conditions were varied as follows: bioluminescence intensity was measured at 5, 10, and 20°С for 175, 100, and 47 h, respectively, at different dose rates (up to 4100 ?Gy/h). There was no noticeable effect of gamma-radiation at 5 and 10°С, while the 20°С exposure revealed authentic bioluminescence inhibition. The 20°С results of gamma-radiation exposure were compared to those for low-dose alpha- and beta-radiation exposures studied previously under comparable experimental conditions. In contrast to ionizing radiation of alpha and beta types, gamma-emission did not initiate bacterial bioluminescence activation (adaptive response). As with alpha- and beta-radiation, gamma-emission did not demonstrate monotonic dose-effect dependencies; the bioluminescence inhibition efficiency was found to be related to the exposure time, while no dose rate dependence was found. The sequence analysis of 16S ribosomal RNA gene did not reveal a mutagenic effect of low-dose gamma radiation. The exposure time that caused 50% bioluminescence inhibition was suggested as a test parameter for radiotoxicity evaluation under conditions of chronic low-dose gamma irradiation. © 2017 Elsevier Ltd

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, Russian Federation
Krasnoyarsk State Agrarian University, 90 Mira Prospect, Krasnoyarsk, Russian Federation
SB RAS Genomics Core Facility, Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russian Federation

Доп.точки доступа:
Kudryasheva, N. S.; Petrova, A. S.; Dementyev, D. V.; Bondar, A. A.

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Is bacterial luminescence response to low-dose radiation associated with mutagenicity? / T. V. Rozhko [et al.] // J. Environ. Radioact. - 2017. - Vol. 177. - P261-265, DOI 10.1016/j.jenvrad.2017.07.010 . - ISSN 0265-931X
Кл.слова (ненормированные):
Bioassay -- DNA -- Low-dose radiation -- Luminous marine bacteria -- Mutations -- Bacteria -- Bioassay -- Bioluminescence -- Chemical activation -- DNA -- DNA sequences -- Genes -- Ionizing radiation -- Kinetics -- Luminescence -- Nucleic acids -- Phosphorescence -- Physiological models -- Radioisotopes -- Bacterial suspensions -- Beta-emitting radionuclides -- Low dose radiation -- Luminescence intensity -- Marine bacterium -- Mutations -- Photobacterium phosphoreum -- Physiological parameters -- Radiation -- Bacteria (microorganisms) -- Photobacterium phosphoreum
Аннотация: Luminous marine bacteria are widely used in bioassays with luminescence intensity being a physiological parameter tested. The purpose of the study was to determine whether bacterial genetic alteration is responsible for bioluminescence kinetics change under low-dose radiation exposure. The alpha-emitting radionuclide 241Am and beta-emitting radionuclide 3H were used as the sources of low-dose ionizing radiation. Changes of bioluminescence kinetics of Photobacterium phosphoreum in solutions of 241Am(NO3)3, 7 kBq/L, and tritiated water, 100 MBq/L, were studied; bioluminescence kinetics stages (absence of effect, activation, and inhibition) were determined. Bacterial suspension was sampled at different stages of the bioluminescent kinetics; the doses accumulated by the samples were close or a little higher than a tentative limit of a low-dose interval: 0.10 and 0.85 Gy for 241Am, or 0.11 and 0.18 Gy for 3H. Sequence analysis of the 16S ribosomal RNA gene did not reveal a mutagenic effect of low-dose alpha and beta radiation in the bacterial samples. Previous results on bacterial DNA exposed to low-dose gamma radiation (0.25 Gy) were analyzed and compared to those for alpha and beta irradiation. It is concluded that bioluminescence activation and/or inhibition under the applied conditions of low-dose alpha, beta and gamma radioactive exposure is not associated with DNA mutations in the gene sequences tested. © 2017 Elsevier Ltd

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Держатели документа:
Krasnoyarsk State Medical Academy, 1 P.Zheleznyaka, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, Russian Federation
SB RAS Genomics Core Facility, Institute of Chemical Biology and Fundamental Medicine SB RAS, 8 Lavrentiev Avenue, Novosibirsk, Russian Federation
Siberian State Technological University, LB, 29 Pobedy, Lesosibirsk, Krasnoyarsk Region, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Rozhko, T. V.; Guseynov, O. A.; Guseynova, V. E.; Bondar, A. A.; Devyatlovskaya, A. N.; Kudryasheva, N. S.

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Author Correction: Plants with genetically encoded autoluminescence (Nature Biotechnology, (2020), 10.1038/s41587-020-0500-9) / T. Mitiouchkina, A. S. Mishin, L. G. Somermeyer [et al.] // Nat. Biotechnol. - 2020, DOI 10.1038/s41587-020-0578-0 . - Article in press. - ISSN 1087-0156
Аннотация: In the version of this article initially published online, a sentence was missing from the Acknowledgements section: The authors would like to acknowledge the work of Genomics Core Facility of the Skolkovo Institute of Science and Technology, which performed the sequencing and bioinformatic analysis. The error has been corrected in the HTML and PDF versions of the article. © 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.

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Держатели документа:
Planta LLC, Moscow, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Institute of Science and Technology Austria, Klosterneuburg, Austria
Pirogov Russian National Research Medical University, Moscow, Russian Federation
Institute of Biophysics, Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Aivok LLC, Zelenograd, Moscow, Russian Federation
Botanical Garden of Lomonosov Moscow State University, Moscow, Russian Federation
Synthetic Biology Group, MRC London Institute of Medical Sciences, London, United Kingdom
Institute of Clinical Sciences, Faculty of Medicine and Imperial College Centre for Synthetic Biology, Imperial College London, London, United Kingdom

Доп.точки доступа:
Mitiouchkina, T.; Mishin, A. S.; Somermeyer, L. G.; Markina, N. M.; Chepurnyh, T. V.; Guglya, E. B.; Karataeva, T. A.; Palkina, K. A.; Shakhova, E. S.; Fakhranurova, L. I.; Chekova, S. V.; Tsarkova, A. S.; Golubev, Y. V.; Negrebetsky, V. V.; Dolgushin, S. A.; Shalaev, P. V.; Shlykov, D.; Melnik, O. A.; Shipunova, V. O.; Deyev, S. M.; Bubyrev, A. I.; Pushin, A. S.; Choob, V. V.; Dolgov, S. V.; Kondrashov, F. A.; Yampolsky, I. V.; Sarkisyan, K. S.

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Single-cell genomics-based analysis reveals a vital ecological role of thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia / Y.-T. Wu, P.-W. Chiang, K. Tandon [et al.] // Microb. Genomics. - 2021. - Vol. 7, Is. 12. - Ст. 000712, DOI 10.1099/mgen.0.000712 . - ISSN 2057-5858
Кл.слова (ненормированные):
Flow cytometry -- Lake Shunet -- Purple sulfur bacteria -- Single-cell genomics -- genomic DNA -- RNA 16S -- Article -- bioinformatics -- carbon metabolism -- Enterobacter -- fluorescence activated cell sorting -- gene amplification -- gene ontology -- high throughput sequencing -- metagenomics -- microbial community -- microbial diversity -- molecular genetics -- nitrogen metabolism -- nonhuman -- nucleotide sequence -- phylogenetic tree -- phylogeny -- polymerase chain reaction -- Sanger sequencing -- Thiocapsa
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes. © 2021 The Authors.

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Держатели документа:
Department of Forestry, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan
Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan
Institute of Biophysics, Siberian Division of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Wu, Y. -T.; Chiang, P. -W.; Tandon, K.; Rogozin, D. Y.; Degermendzhy, A. G.; Tang, S. -L.

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Single- cell genomics-based analysis reveals a vital ecological role of Thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia / Y. T. Wu, P. W. Chiang, K. Tandon [et al.] // Microb. Genomics. - 2021. - Vol. 7, Is. 12. - Ст. 000712, DOI 10.1099/mgen.0.000712. - Cited References:69. - This research was funded by the Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project Grant NSC102--2923-B--001--004--MY3 and by the Russian Foundation for Basic Research Grant (project no. 21--54--52001) and MOST (105--2923--B--001--001--MY3). . - ISSN 2057-5858

РУБ Genetics & Heredity + Microbiology
Рубрики:
PHOTOTROPHIC BACTERIAL COMMUNITY
PURPLE SULFUR BACTERIUM
SP NOV.
Кл.слова (ненормированные):
flow cytometry -- Lake Shunet -- purple sulfur bacteria -- single-cell -- genomics
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic-anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deepsequencing analysis of single- cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co- assembly of five single- cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes.

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Держатели документа:
Natl Pingtung Univ Sci & Technol, Dept Forestry, Pingtung 91201, Taiwan.
Acad Sinica, Biodivers Res Ctr, Taipei 115, Taiwan.
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Wu, Yu-Ting; Chiang, Pei-Wen; Tandon, Kshitij; Rogozin, Denis Yu; Degermendzhy, Andrey G.; Tang, Sen-Lin; Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project [NSC102-2923-B-001-004-MY3]; Russian Foundation for Basic Research GrantRussian Foundation for Basic Research (RFBR) [21-54-52001]; MOST [105-2923-B-001-001-MY3]

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