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Effect of way of cooking on content of essential polyunsaturated fatty acids in muscle tissue of humpback salmon (Oncorhynchus gorbuscha) / M. I. Gladyshev [et al.] // Food Chemistry. - 2006. - Vol. 96, Is. 3. - P446-451, DOI 10.1016/j.foodchem.2005.02.034 . - ISSN 0308-8146
Кл.слова (ненормированные):
antioxidant -- docosahexaenoic acid -- essential fatty acid -- icosapentaenoic acid -- omega 3 fatty acid -- polyunsaturated fatty acid -- animal tissue -- conference paper -- controlled study -- cooking -- ecological niche -- evolution -- fish -- fluid intake -- food analysis -- freezing -- frying -- heat treatment -- heating -- hypothesis -- market -- muscle tissue -- nonhuman -- oncorrhynchus gorbuscha -- reduction -- Russian Federation -- salmon -- statistical significance -- Animalia -- Oncorhynchus gorbuscha -- Pisces -- Salmonidae
Аннотация: Contents of fatty acids in filets of unfrozen (control), boiled, fried, roasted and boiled in a small amount of water humpback salmon, collected from a wholesale market in Krasnoyarsk city (Siberia, Russia) were analyzed. Special attention was paid to essential polyunsaturated fatty acids of ?3 family: eicosapentaenoic, 20:5?3 (EPA) and docosahexaenoic, 22:6?3 (DHA). Heat treatment in general did not decrease content of EPA and DHA in humpback, except a modest reduction during frying. Cooked humpback appeared to be the valuable source of essential ?3 PUFAs, namely EPA and DHA. It was hypothesized that the absence of significant reduction of PUFAs' contents in red flesh of fishes of Salmonidae family during heat treatment may be due to a high level of natural antioxidants which formed in the course of evolution as adaptation to their ecological niche. В© 2005 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Krasnoyarsk State Trade-Economical Institute, Lidiya Prushinskaya Street, 2, Krasnoyarsk 660075, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Gubanenko, G.A.; Demirchieva, S.M.; Kalachova, G.S.

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EFFECT OF TEMPERATURE ON ACTIVITY AND STABILITY OF OBELIN [Text] / V. S. BONDAR [et al.] // Biochem.-Moscow. - 1992. - Vol. 57, Is. 7. - P717-724. - Cited References: 15 . - 8. - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology
Рубрики:
CA-2+
PHOTOPROTEINS
INDICATORS
Кл.слова (ненормированные):
PHOTOPROTEINS -- OBELIN -- ACTIVATION ENERGY -- THERMOINACTIVATION -- THERMOSTABILITY
Аннотация: The temperature dependence of bioluminescent activity of the Ca2+-activated photoprotein obelin from the hydroid polyp Obelia longissima and thermoinactivation of this protein at different concentrations of (NH4)2SO4 have been studied. The maximal intensity of luminescence of obelin was observed at 4-15-degrees-C. The activity of the photoprotein is completely stable to storage for 3 days at room temperature. Increasing the temperature to 40-degrees-C resulted in a 25-30% loss of enzyme activity in 1 h. The presence of ammonium sulfate during heating stabilizes the activity of obelin. Two breaks, at 11 +/- 3-degrees-C and 47 +/- 3-degrees-C, are observed in the Arrhenius plot of the first-order rate constant of the luminescence decay. The bioluminescent curves of obelin are biphasic in the temperature range 10-40-degrees-C. It is assumed that obelin may exist in two kinetically distinct conformers (active and inactive) whose ratio is temperature dependent.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
BONDAR, V.S.; TROFIMOV, K.P.; SANDALOV, T.P.; VYSOTSKII, E.S.

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Recombinant Metridia luciferase isoforms: expression, refolding and applicability for in vitro assay [Text] / V. V. Borisova [et al.] // Photochem. Photobiol. Sci. - 2008. - Vol. 7, Is. 9. - P1025-1031, DOI 10.1039/b807271j. - Cited References: 19. - The work was supported by Bayer AG, by the Russian Foundation for Basic Research grants 05-04-48271 and 06-04-08076, by the joint grant 06-04-89502 of the Russian Foundation for Basic Research and Taiwan National Science Council, and by the "Molecular and Cellular Biology" program of the Russian Academy of Sciences. . - ISSN 1474-905X

РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical
Рубрики:
BIOLUMINESCENT REPORTER
   GAUSSIA LUCIFERASE
   CDNA
   PROTEINS
   CLONING
   OVEREXPRESSION
   PURIFICATION
   MUTAGENESIS
   ENZYME
   OBELIN
Аннотация: The recombinant coelenterazine-dependent luciferases (isoforms MLuc 164 and MLuc39) from the marine copepod Metridia longa were expressed as inclusion bodies in E. coli cells, dissolved in 6 M guanidinium chloride and folded in conditions developed for proteins containing intramolecular disulfide bonds. One of them (MLuc09) was obtained in an active monomeric form with a high yield. The luciferase bioluminescence is found to be initiated not only by free coelenterazine, but also by Ca2+-dependent coelenterazine-binding protein (CBP) of Renilla muelleri on Ca2+ addition. The use of CBP as a "substrate" provides higher light emission and simultaneously the lower level of background. The high purity MLuc39 can be detected down to attomol with a linear range extending over 5 orders of magnitude. The MLuc39 reveals also a high stability towards heating and chemical modification; the chemically synthesized biotinylated derivatives of the luciferase preserve 35-40% of the initial activity The luciferase applicability as an in vitro bioluminescent reporter is demonstrated in model tandem bioluminescent solid-phase microassay combining the Ca2+-regulated photoprotein obelin and the Metridia luciferase.

Держатели документа:
[Borisova, Vasillisa V.
Frank, Ludmila A.
Markova, Svetlana V.
Burakova, Ludmilla P.
Vysotski, Eugene S.] Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk 660036, Russia
[Frank, Ludmila A.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Borisova, V.V.; Frank, L.A.; Markova, S.V.; Burakova, L.P.; Vysotski, E.S.

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Ca2+-triggered coelenterazine-binding protein from Renilla as an enzyme-dependent label for binding assay [Text] / V. V. Krasitskaya [et al.] // Anal. Bioanal. Chem. - 2011. - Vol. 401, Is. 8. - P2573-2579, DOI 10.1007/s00216-011-5343-2. - Cited References: 17. - The work was supported by a "Leading Scientific School" (N 64987.2010.4) grant from the President of the Russian Federation and the "Molecular and Cell Biology" Program from the RAS. . - ISSN 1618-2642

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
BIOLUMINESCENT IMMUNOASSAY
   LUCIFERASE
   PURIFICATION
   RENIFORMIS
   MUELLERI
   OBELIN
   PHOTOPROTEIN
   EXPRESSION
   SUBSTRATE
   CLONING
Кл.слова (ненормированные):
Ca2+-triggered coelenterazine-binding protein (CBP) -- Renilla muelleri luciferase -- Bioluminescent solid-phase microassay
Аннотация: The recombinant Ca2+-triggered coelenterazine-binding protein (CBP) from Renilla muelleri was investigated as a biospecifically labeled molecule for in vitro assay applications. The protein was shown to be stable in solutions in the frozen state, as well as stable under heating and to chemical modifications. Conjugates with biotin, oligonucleotide, and proteins were obtained and applied as biospecific molecules in a solid-phase microassay. CBP detection was performed with intact (no modifications were made) Renilla luciferase in the presence of calcium, and the detection limit was found to be 75 amol. Model experiments indicate that this approach shows much promise, especially with regard to the development of multianalytical systems.

Держатели документа:
[Korneeva, S. I.
Kudryavtsev, A. N.
Frank, L. A.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Krasitskaya, V. V.
Markova, S. V.
Stepanyuk, G. A.
Frank, L. A.] Russian Acad Sci SB, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krasitskaya, V.V.; Korneeva, S.I.; Kudryavtsev, A.N.; Markova, S.V.; Stepanyuk, G.A.; Frank, L.A.

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Assessment of the Volatility and Thermal Stability of Chemicals that Stimulate Females of Moina macrocopa (Cladocera) to Produce Diapausing Eggs [Text] / T. S. Lopatina, E. S. Zadereev // Russ. J. Ecol. - 2015. - Vol. 46, Is. 1. - P103-108, DOI 10.1134/S1067413615010105. - Cited References:25 . - ISSN 1067-4136. - ISSN 1608-3334

РУБ Ecology
Рубрики:
SEXUAL REPRODUCTION
   INDUCTION
   DAPHNIA
   METABOLITES
   CRUSTACEA
Кл.слова (ненормированные):
cladocerans -- diapause -- chemical communication -- chemical cues
Аннотация: The volatility and thermal stability of chemicals that are contained in the metabolic products of Moina macrocopa females that stimulate the production of diapausing eggs in these females have been studied in experiments with separately cultivated individuals. It has been shown that the gametogenesis-stimulating chemicals in this species are nonvolatile compounds: the effect of the medium that contains the metabolic products of the organism was not decreased after many hours of blowing with argon. Prolonged heating up to 80 degrees C also did not deactivate the ability of these products to change the reproductive mode. The effect of the medium that contains these products decreased after boiling, but if the possibilities of their oxidation or binding with salts of metals were excluded, they retained the ability to change the reproductive mode of the animals even after boiling.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Lopatina, T.S.; Zadereev, E.S.

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Contrasting relationship between macro- and microviscosity of the gelatin- and starch-based suspensions and gels [Text] / D. V. Gulnov, E. V. Nemtseva, V. A. Kratasyuk // Polym. Bull. - 2016. - Vol. 73, Is. 12. - P3421-3435, DOI 10.1007/s00289-016-1664-9. - Cited References:42. - Authors thank Alexander Kheruvimov (REC "Composite Materials and Structures", SUSU, Chelyabinsk, Russia) for assistance in rheological experiments. The research was partially supported by the grants No. 11.G34.31.0058 and 1762 from The Ministry of Education and Science of the Russian Federation and by the state budget allocated to the fundamental research at the Russian Academy of Sciences (Project No. 01201351504). . - ISSN 0170-0839. - ISSN 1436-2449

РУБ Polymer Science
Рубрики:
FLUORESCENT MOLECULAR ROTORS
INTRACELLULAR VISCOSITY
DRUG-DELIVERY
Кл.слова (ненормированные):
Biopolymer -- Gelatin -- Starch -- Physical gel -- Microviscosity -- Molecular -- rotor
Аннотация: The problem of correlation between rheological properties in macro- and micro- scales of media with biopolymers of polypeptide (gelatin) and polysaccharide (starch) nature is investigated. The viscosity of the biopolymer solutions with concentrations 0.5-5 wt% was estimated by standard rotational rheometry technique and with fluorescent molecular rotor at 15-50 A degrees C. Opposite trends were observed for relationship between microviscosity eta (m) and macroviscosity eta for two biopolymers: eta (m) << eta for gelatin and eta (m) >> eta for starch solutions. The temperature dependence of eta (m) followed the monoexponential decay law in all samples over the whole temperature range indicating insensitivity of microviscosity to gel mesh melting under heating. The dissimilarity of macro- and micro-rheological properties of gelatin and starch-containing media is discussed in terms of difference in architecture of the gels.

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Держатели документа:
Siberian Fed Univ, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Gulnov, Dmitry V.; Nemtseva, Elena V.; Kratasyuk, Valentina A.; Ministry of Education and Science of the Russian Federation [11.G34.31.0058, 1762]; Russian Academy of Sciences [01201351504]

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Microbial transformation of carbon CH4 and CO2 in permafrost-affected soils in tundra and forest ecosystems in Siberia / I. D. Grodnitskaya [и др.] // Russ. J. For. Sci. - 2017. - Is. 2. - С. 111-127 . - ISSN 0024-1148
Кл.слова (ненормированные):
Bacterial diversity -- CH4 and CO2 emission -- Dynamics and activity of microbial complexes -- Frost-crack polygons -- Larch forests -- Tundra -- Сryogenic soils
Аннотация: We studied structure, dynamics and functional (biogeochemical) activity of microbial complexes of cryogenic soils in larch forests in Central Evenkia and polygonal tundra on Samoilovskii Island, Lena Delta. We found that daily flux of methane from soil surface is 3–5 times less in forest soil than in the center of polygon in tundra. Short-term heating to 18.5–22.5 °C of permafrost-affected soil in larch forest caused sweetening of soil solution, shrinkage of eco-trophic groups of microorganisms and microbial biomass, as well as increase in greenhouse gases (CO2 and CH4) emission to the air. Notably the permafrost-affected soil on sandy deposits in tundra had highest microbial diversity of methanogenic archaea including Methanobacteriaceae, Methanomicrobiaceae, Methanosarcinaceae, Methanosaetaceae families. On the other hand only Methanosarcinacea were found in cryosols of larch forest. Both type I and type II methanotrophs were found in the forest soil, while only type II methanotrophs occurred in tundra soil. © 2017, Izdatel’stvo Nauka. All rights reserved.

Scopus
Держатели документа:
Forest Institute, Siberian Branch of the Russian Academy of Sciences, Academgorodok, 50, bldg. 28, Krasnoyarsk, Russian Federation
Krasnoyarsk filial of the Information and Methodological Center for Expert Evaluation, Recording and Analysis of Circulation of Medical Products, Roszdravnadzor, Kutuzova st., 1, bldg. 1, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Academgorodok, 50, bldg. 50, Krasnoyarsk, Russian Federation
Roche Diagnostika Rus Ltd., Letnikovskaya, 2, bldg. 2, Moscow, Russian Federation

Доп.точки доступа:
Grodnitskaya, I. D.; Sorokin, N. D.; Evgrafova, S. Y.; Antonov, G. I.; Syrtsov, S. N.; Aleksandrov, D. E.; Trusova, M. Y.; Koroban, N. V.

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The Influence of Temperature and Humidity on Greenhouse Gas Emission in Experiments on Imitation of the Full Vegetation Cycle of Tundra Ecosystems / Y. V. Barkhatov [et al.] // Dokl. Earth Sci. - 2018. - Vol. 483, Is. 2. - P1539-1541, DOI 10.1134/S1028334X18120115. - Cited References:15. - This study was supported by the Russian Foundation for Basic Research, the Krasnoyarsk Krai Government and the Krasnoyarsk Regional Fund of Science (project no. 17-45-240884), the Russian Foundation for Basic Research (project no. 16-04-01677-a), and the Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, governmental assignment, theme no. 56.1.4. for 2013-2020. . - ISSN 1028-334X. - ISSN 1531-8354

РУБ Geosciences, Multidisciplinary
Рубрики:
POLYGONAL TUNDRA
   METHANE EMISSION
   CARBON
   DELTA
   LAKES
Аннотация: Laboratory experiments were conducted in a hermetically sealed growth chamber with two soil samples obtained from the arctic tundra zone with different levels of moisture. Samples were maintained at a growing season typical of the region from which they were taken, and for the sample with a high level of moisture it was made twice: with the temperature in accord with natural conditions and one increased by 2 degrees C. It has been shown that heating of the overmoistened tundra soil by 2 degrees C can increased the average carbon dioxide emissions by almost two times (from 75 to 100-150 mg m(-2) h(-1)). Upon the application of heat, no significant increase in methane emission was observed.

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Scopus
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Sukachev Inst Forests, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Barkhatov, Yu. V.; Ushakova, S. A.; Shikhov, V. N.; Evgrafova, S. Yu.; Tikhomirov, A. A.; Degermendzhi, A. G.; Russian Foundation for Basic Research; Krasnoyarsk Krai Government; Krasnoyarsk Regional Fund of Science [17-45-240884]; Russian Foundation for Basic Research [16-04-01677-a]; Institute of Biophysics, Siberian Branch, Russian Academy of Sciences [56.1.4.]

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