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An in vivo study of pha matrices of different chemical composition: Tissue reaction and biodegradation / E. I. Shishatskaya [et al.] // Cellular Transplantation and Tissue Engineering. - 2012. - Vol. 7, Is. 1. - P73-80 . - ISSN 1815-445X
Кл.слова (ненормированные):
Degradable polymer matrices -- Degradation -- PHAs -- Polyhydroxyalkanoates -- Subcutaneous implantation -- Tissue reaction
Аннотация: The study addresses consequences of subcutaneous implantation of film matrices prepared from different PHAs to laboratory animals. No negative effects of subcutaneous implantation of PHA matrices on physiological and biochemical characteristics of the animals were determined. Independently of the matrices composition and duration of the contact with the internal environment of the organism we did not observe any deviations in the behavior of animals, their growth and development, as well as blood functions. Response of the tissues to PHA matrices was comparable with the response to polylactide, but substantially less expressed at the earlier time periods after implantation. Tissues response to implantation of PHA of all types is characterized by short-term (up to 2 weeks) post-traumatic inflammation with formation of fibrous capsules by 30th-60th days with the thickness less than 100 microns, which get thinner down to 40-60 microns by 180th day as the result of involution. No differences in response of tissues and the whole organism were observed for the matrices produced from the homopolymer of 3-hydroxybutyric acid (P3HB), copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate acids (P3HB/3HH). Macrophages and foreign-body giant cells actively participate in the response of the tissues to PHAs. In the studied conditions matrices from the copolymers containing 3-hydroxyhexanoate and 4 hydroxybutyrate were determined as more actively degraded PHA. The next less degraded matrices were matrices from the copolymer of P3HB/3HV and the most resistant were P3HB matrices. The slower degradation of PHA matrices was accompanied by delayed development of giantcells response. The studied PHA matrices can be placed in the following range by their degradation: P3HB/3HH - P3HB/4HB - P3HB/HV - P3HB.

Scopus
Держатели документа:
Institute of Modern Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Massachusetts Institute of Technology, Cambridge, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Nikolaeva, E.D.; Goreva, A.V.; Brigham, C.J.; Volova, T.G.; Sinskey, A.J.

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Biocompatibility of polyhydroxybutyrate microspheres: In vitro and in vivo evaluation / E. I. Shishatskaya [et al.] // Journal of Materials Science: Materials in Medicine. - 2008. - Vol. 19, Is. 6. - P2493-2502, DOI 10.1007/s10856-007-3345-6 . - ISSN 0957-4530
Кл.слова (ненормированные):
Biocompatibility -- Drug delivery -- Fibroblasts -- Ion implantation -- Microspheres -- Polymer matrix -- Fibroblast cells -- Hydroxybutyric acid -- Intramuscular implantation -- Polyhydroxybutyrate -- Organic polymers -- microsphere -- poly(3 hydroxybutyric acid) -- polymer -- animal cell -- animal experiment -- animal tissue -- article -- biocompatibility -- cell infiltration -- controlled study -- giant cell -- implantation -- in vitro study -- in vivo study -- inflammation -- macrophage -- mouse -- nonhuman -- priority journal -- rat -- 3-Hydroxybutyric Acid -- Animals -- Biocompatible Materials -- Cell Survival -- Inflammation -- Materials Testing -- Mice -- Microspheres -- Necrosis -- NIH 3T3 Cells -- Polymers -- Rats -- Rats, Wistar -- Tetrazolium Salts -- Thiazoles -- Time Factors -- Rattus norvegicus
Аннотация: Microspheres have been prepared from the resorbable linear polyester of ?-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection. В© 2008 Springer Science+Business Media, LLC.

Scopus
Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Laboratory of Bacterial Bioluminescence, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svo Bodnyi Av. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Voinova, O.N.; Goreva, A.V.; Mogilnaya, O.A.; Volova, T.G.

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Tissue reaction to intramuscular injection of resorbable polymer microparticles / E. I. Shishatskaya [et al.] // Bulletin of Experimental Biology and Medicine. - 2007. - Vol. 144, Is. 6. - P786-790, DOI 10.1007/s10517-007-0432-0 . - ISSN 0007-4888
Кл.слова (ненормированные):
Microencapsulation -- Polyhydroxybutyrate -- Resorbable polymers -- Tissue reaction -- poly(3 hydroxybutyric acid) -- polymer -- animal experiment -- animal tissue -- article -- cell infiltration -- controlled study -- drug delivery system -- drug formulation -- female -- giant cell -- inflammation -- macrophage -- microencapsulation -- nonhuman -- rat -- tissue reaction -- Absorbable Implants -- Animals -- Drug Compounding -- Female -- Foreign-Body Reaction -- Injections, Intramuscular -- Microspheres -- Polyesters -- Rats -- Rats, Wistar
Аннотация: Tissue reaction to implantation of polymeric microparticles from resorbable polymer (polyhydroxybutyrate) is characterized by slight inflammatory reaction and pronounced progressive macrophage infiltration with the presence of mono-and multinuclear foreign body giant cells resorbing the polymeric matrix. No fibrous capsules were formed around the polymeric microparticles; neither necrosis nor other adverse morphological changes and tissue transformation in response to implantation of the PHB microparticles were recorded. The results indicate good prospects of using polyhydroxybutyrate for the construction of long-acting dosage forms as microparticles for intramuscular injection. В© Springer Science+Business Media, Inc. 2007.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Voinova, O.N.; Goreva, A.V.; Mogilnaya, O.A.; Volova, T.G.

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Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures / E. I. Shishatskaya [et al.] // Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - P719-728, DOI 10.1023/B:JMSM.0000030215.49991.0d . - ISSN 0957-4530
Кл.слова (ненормированные):
Necrosis -- Polymeric implants -- Suppurative inflammation -- Tissue reaction -- Biodegradation -- Calcification (biochemistry) -- Cells -- Copolymers -- Implants (surgical) -- Silk -- Tissue -- Tumors -- Materials science -- acid phosphatase -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polyhydroxyvaleric acid -- unclassified drug -- animal experiment -- animal model -- article -- biodegradable implant -- blood vessel reactivity -- catgut -- controlled study -- enzyme activity -- female -- giant cell -- histochemistry -- inflammation -- macrophage -- nonhuman -- priority journal -- rat -- silk -- suture -- tensile strength -- tissue reaction -- tissue structure -- wound healing -- young modulus -- Absorbable Implants -- Animals -- Female -- Fibrosis -- Foreign-Body Reaction -- Hydroxybutyrates -- Muscle, Skeletal -- Polyesters -- Polymers -- Rats -- Rats, Wistar -- Sutures -- Treatment Outcome -- Wounds, Penetrating -- Animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.

Scopus
Держатели документа:
Inst. Biophys. Siberian Br. Russ. A., Akademgorodok, Krasnoyarsk 660036, Russia, Russian Federation
Terr. Pathological Anatomy Bureau, Partisan Zheleznyak St. 1, Krasnoyarsk 660049, Russia, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Puzyr, A.P.; Mogilnaya, O.A.; Efremov, S.N.

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Results of biomedical investigations of PHB and PHB/PHV fibers / T. Volova [et al.] // Biochemical Engineering Journal. - 2003. - Vol. 16, Is. 2. - P125-133, DOI 10.1016/S1369-703X(03)00038-X . - ISSN 1369-703X
Кл.слова (ненормированные):
Biocompatibility -- Fibers -- Implants -- Poly-3-hydroxybutyrate (PHB) -- Poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV) -- Polyhydroxyalkanoates (PHAs) -- Tissue reaction -- Bacteriology -- Calcification (biochemistry) -- Copolymers -- Flammability -- Tissue -- Tissue response -- Biomedical engineering -- acid phosphatase -- poly(3 hydroxybutyric acid) -- poly(3 hydroxybutyric acid) co poly(3 hydroxyvaleric acid) -- polyhydroxyalkanoic acid -- suture material -- unclassified drug -- animal experiment -- article -- bacterial metabolism -- biochemistry -- biocompatibility -- biomedicine -- calcification -- catgut -- correlation analysis -- dose time effect relation -- enzyme activity -- fascia -- fiber -- foreign body -- functional assessment -- giant cell -- implantation -- in vivo study -- inflammation -- intermethod comparison -- macrophage -- materials testing -- multinuclear cell -- muscle injury -- necrosis -- nonhuman -- parameter -- phagocytosis -- physiology -- postoperative period -- priority journal -- process monitoring -- ralstonia eutropha b 5786 -- scar formation -- silk -- strength -- suture -- synthesis -- tissue reaction -- Wautersia eutropha -- wound healing -- Animalia -- Ralstonia -- Wautersia eutropha
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 ?m thick during weeks 4-8, which in 4-6 months was reduced to 40-60 ?m in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. В© 2003 Elsevier Science B.V. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Inst. Transplantology Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Terr. Pathological Anatomy Bureau, Partisan Zheleznyak St. 1, Krasnoyarsk 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Shishatskaya, E.; Sevastianov, V.; Efremov, S.; Mogilnaya, O.

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In vitro justification of using endobiliary stents made of polyhydroxyalkanoates / N. M. Markelova [et al.] // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P82-91, DOI 10.1002/masy.200850910 . - ISSN 1022-1360
Кл.слова (ненормированные):
Bioresorbable materials -- Endobiliary stents -- Mechanical jaundice (MJ) -- PHAs -- ABS resins -- Biopolymers -- Biotechnology -- Body fluids -- Experiments -- Free radical polymerization -- Liver -- Polymers -- Prosthetics -- Silicon -- Surgery -- Turbulent flow -- Abdominal cavities -- Bile ducts -- Biliary -- Biological properties -- Bioresorbable materials -- Blood analyses -- Endobiliary stents -- Endoprosthesis -- Experimental groups -- Experimental models -- In vitro -- Inflammatory reactions -- Macroscopic changes -- Mechanical jaundice (MJ) -- Minimally invasive -- Negative controls -- PHAs -- Polyhydroxyalkanoates -- Positive control groups -- Radical surgeries -- Reconstructive surgeries -- Suture materials -- Biological materials
Аннотация: Treatment of patients with mechanical jaundice (MJ) has been one of the topical problems of current medicine. The fraction of patients having MJ of oncologic character that can undergo radical surgery does not exceed 25-30%. A way out of such situation is to use minimally invasive endobiliary interventions, the main type of which is endoprosthesis replacement of bile duct lumens. The aim of the work was to study the biological properties of the experimental models of polymer stents made of PHA for endobiliary prosthetics and to investigate the biological properties of PHA suture material for forming of biliary-enteric anastomoses (cholecystoduodenostomy). Experiments were performed on 20 adult mongrel dogs, weighing 10-12 kg. The animals were divided to three groups: the negative control group (intact animals); the positive control group (the animals with implanted endobiliary silicon stents); and the experimental group (the animals with the PHA stents). The animals were monitored for 100 days. The clinical blood analysis was made before the operation, on day 7, 30, 60 and 100 days. During autopsy the presence of exudate, commissural process in the free abdominal cavity and subhepatic spatium, the appearance of choledoch where the prosthesis was located, as well as the appearance of cholecystoenterostomy, liver and duodenum. We did not found any signs of inflammation, cicatrical changes were estimated in the free abdominal cavity and subhepatic spatium. All implanted PHA stents were at their initial places of implantation. After the end of the experiment inflammatory reaction and anastomositis were absent. Macroscopic changes of liver and duodenum were also not detected. Liver function did not have any pathological deviations. These positive results give grounds to conclude that application of PHA as endobiliary stents in reconstructive surgery of bile passages and as suture material is a promising technology. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Krasnoyarsk State Medical Academy, Partizana Zheleznyaka Street, 1, 660022, Krasnoyarsk, Russian Federation
Institute of Biophysics, SB, RAS, Akademgorodok, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Markelova, N.M.; Shishatskaya, E.I.; Vinnik, Y.S.; Cherdantsev, D.V.; Beletskiy, I.I.; Kuznetsov, M.N.; Zykova, L.D.

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Results of biomedical investigations of PHB and PHB/PHV fibers [Text] / T. . Volova [et al.] // Biochem. Eng. J. - 2003. - Vol. 16, Is. 2. - P. 125-133, DOI 10.1016/S1369-703X(03)00038-X. - Cited References: 20 . - ISSN 1369-703X

РУБ Biotechnology & Applied Microbiology + Engineering, Chemical
Рубрики:
POLYHYDROXYALKANOATES
POLYESTERS
POLYMERS
Кл.слова (ненормированные):
polyhydroxyalkanoates (PHAs) -- poly-3-hydroxybutyrate (PHB) -- poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV) -- fibers -- implants -- biocompatibility -- tissue reaction
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 mum thick during weeks 4-8, which in 4-6 months was reduced to 40-60 mum in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. (C) 2003 Elsevier Science B.V. All rights reserved.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Akademgorodok, Krasnoyarsk 60036, Russia
Russian Minist Hlth, Inst Transplantol Artificial Organs, Moscow 123182, Russia
Territorial Pathol Anat Bur, Krasnoyarsk 660049, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Shishatskaya, E...; Sevastianov, V...; Efremov, S...; Mogilnaya, O...

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Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures [Text] / E. I. Shishatskaya [et al.] // J. Mater. Sci.-Mater. Med. - 2004. - Vol. 15, Is. 6. - P. 719-728, DOI 10.1023/B:JMSM.0000030215.49991.0d. - Cited References: 34 . - ISSN 0957-4530

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DEGRADATION
   POLYESTERS
   POLYMERS
   FIBERS
   PHB
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types-polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV)-featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 mum thick, which became as thin as 40-60 mum after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner. (C) 2004 Kluwer Academic Publishers.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
Territorial Pathol Anat Bur, Krasnoyarsk 660049, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Puzyr, A.P.; Mogilnaya, O.A.; Efremov, S.N.

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Experimental wound dressings of degradable PHA for skin defect repair / E. I. Shishatskaya [et al.] // J. Mater. Sci. Mater. Med. - 2016. - Vol. 27, Is. 11, DOI 10.1007/s10856-016-5776-4 . - ISSN 0957-4530
Кл.слова (ненормированные):
Biomaterials -- Cell culture -- Cells -- Cytology -- Enzyme inhibition -- Fibroblasts -- Membranes -- Natural polymers -- Nonwoven fabrics -- Polymer films -- Proteins -- Stem cells -- Weaving -- Electrospun membranes -- Extracellular matrix protein -- Fibroblast cells -- Hydroxyderivative -- Mesenchymal stem cell -- Non-woven membranes -- Wound dressing materials -- Wound healing process -- Tissue
Аннотация: The present study reports construction of wound dressing materials from degradable natural polymers such as hydroxy derivatives of carboxylic acids (PHAs) and 3-hydroxybutyrate/4-hydroxybutyrate [P(3HB/4HB)] as copolymer. The developed polymer films and electrospun membranes were evaluated for its wound healing properties with Grafts—elastic nonwoven membranes carrying fibroblast cells derived from adipose tissue multipotent mesenchymal stem cells. The efficacy of nonwoven membranes of P(3HB/4HB) carrying the culture of allogenic fibroblasts was assessed against model skin defects in Wistar rats. The morphological, histological and molecular studies revealed the presence of fibroblasts on dressing materials which facilitated wound healing, vascularization and regeneration. Further it was also observed that cells secreted extracellular matrix proteins which formed a layer on the surface of membranes and promoted the migration of epidermal cells from the neighboring tissues surrounding the wound. The wounds under the P(3HB/4HB) membrane carrying cells healed 1.4 times faster than the wounds under the cell-free membrane and 3.5 times faster than the wounds healing under the eschar (control).The complete wound healing process was achieved at Day 14. Thus the study highlights the importance of nonwoven membranes developed from degradable P(3HB/4HB) polymers in reducing inflammation, enhancing angiogenic properties of skin and facilitating better wound healing process. © 2016, Springer Science+Business Media New York.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50-50 Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodniy Ave., Krasnoyarsk, Russian Federation

Доп.точки доступа:
Shishatskaya, E. I.; Nikolaeva, E. D.; Vinogradova, O. N.; Volova, T. G.

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10.

Is Body Mass Index a potential biomarker for anemia in obese adolescents? / S. Pande, R. Ranjan, V. A. Kratasyuk // J. Nutr. Intermediary Metab. - 2019. - Vol. 15. - P1-2, DOI 10.1016/j.jnim.2018.11.001 . - ISSN 2352-3859
Кл.слова (ненормированные):
Anemia -- Body Mass Index -- Hepcidin -- Leptin -- Obesity
Аннотация: The two paradoxical major health problems namely obesity and anemia are confirmed to affect millions around the world. Hepcidin, a protein synthesized in liver is a negative iron binding regulator. There is an affirmative relation between hepcidin and leptin levels and an inverse co-relation between hepcidin and iron status due to inflammation mediated by obesity in adolescents. So this implicates an alliance between anemia and obesity wherein weight reduction can be a powerful medium to improve iron absorption in obese adolescents. Also the Body Mass Index can serve as a preliminary non-invasive screening tool to identify potential adolescents prone to anemia. © 2018

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Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Pande, S.; Ranjan, R.; Kratasyuk, V. A.

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11.

Nutritional biomarkers: Current view and future perspectives / S. Pande [et al.] // Crit. Rev. Food Sci. Nutr. - 2018. - Vol. 58, Is. 18. - P3055-3069, DOI 10.1080/10408398.2017.1350136. - Cited References:163. - The research was partially supported by the Russian Foundation for Basic Research [Grant No 16-06-00-439], the Russian Ministry of Education, Post-Doctoral Program of Project "5-100" [Grant No. M 2.2.3] and the Russian Foundation for Basic Research in collaboration with Krasnoyarsk Regional Fund for Scientific and Technical Activity Support [project No 16-44-242126]. . - ISSN 1040-8398. - ISSN 1549-7852

РУБ Food Science & Technology + Nutrition & Dietetics
Рубрики:
PROMOTES CELL-SURVIVAL
FATTY-ACID OXIDATION
CALORIE RESTRICTION
Кл.слова (ненормированные):
Sirtuins -- NAD -- biomarker -- proteomics -- calorie restriction -- longitivity
Аннотация: There is a poor relationship between nutrient intake and existing nutritional biomarkers due to variety of factors affecting their sensitivity and specificity. To explore the impact of nutrients at molecular level and devising a sensitive biomarker, proteomics is a central technology with sirtuins as one of the most promising nutritional biomarker. Sirtuins (seven mammalian sirtuins reported so far) have been reported to perform protein deacetylases and ADP-ribosyltransferases activity. It is distributed in different cellular compartments thereby controlling several metabolic processes. Sirtuins are oxidized nicotinamide adenine dinucleotide dependent, which implicates a direct effect of the metabolic state of the cell on its activity. Calorie restriction upregulates the mammalian sirtuin protein levels in variety of tissues and organs where it acts upon both histone and nonhistone substrates. Sirtuin senses nutrient availability and impacts gluconeogenesis, glycolysis, and insulin sensitivity. It deacetylates and inhibits the nuclear receptor that activates fat synthesis and adipogenesis in the body, leading to fat loss and bringing favorable cellular and health changes. Sirtuins mediates intracellular response that promotes cell survival, DNA damage repair thereby increasing the cell longitivity. The activation of sirtuins brings a wide spectrum of other health benefits and its activity levels are indicative of nutritional status as well as disease progression in cancer, inflammation, obesity, cardiovascular diseases, and viral infections. There are several foods that activate sirtuin activity and offer significant health benefits by their consumption.

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Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Lab Bioluminescent Biotechnol, Krasnoyarsk, Russia.
Krasnoyarsk State Med Univ, Krasnoyarsk, Russia.
RAS, Siberian Branch, Inst Biophys, Fed State Budgetary Sci Inst, Krasnoyarsk, Russia.
Fed State Budgetary Sci Inst, Sci Res Inst Med Problems North, Krasnoyarsk, Russia.

Доп.точки доступа:
Pande, Shubhra; Kratasyuk, Valentina A.; Medvedeva, Nadezhda N.; Kolenchukova, Oxana A.; Salmina, Alla B.; Kratazyuk, Valentina; KAYITESI, EUGENIE; Russian Foundation for Basic Research [16-06-00-439]; Russian Ministry of Education [M 2.2.3]; Russian Foundation for Basic Research; Krasnoyarsk Regional Fund for Scientific and Technical Activity Support [16-44-242126]

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12.

Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB) / T. G. Volova [et al.] // Int. J. Biol. Macromol. - 2019. - Vol. 131. - P230-240, DOI 10.1016/j.ijbiomac.2019.03.068. - Cited By :1 . - ISSN 0141-8130
Кл.слова (ненормированные):
Bacterial cellulose -- Composites -- Polyhydroxyalkanoates -- 3 hydroxybutyric acid 4 hydroxybutyric acid copolymer -- actovegin -- bacterial cellulose -- cellulose -- collagen type 1 -- copolymer -- cytokeratin 10 -- cytokeratin 14 -- solcoseryl -- unclassified drug -- angiogenesis -- animal cell -- animal experiment -- animal model -- animal tissue -- biochemical analysis -- biocompatibility -- burn -- cell growth -- controlled study -- cutaneous parameters -- differential scanning calorimetry -- drug release -- elemental analysis -- female -- fibroblast -- histopathology -- in vitro study -- in vivo study -- inflammation -- mechanics -- nonhuman -- rat -- Review -- wound healing -- wound planimetry -- X ray analysis
Аннотация: Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] – a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings – BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts – were tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPran – a commercial wound dressing. © 2019

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizan Zheleznyak Street, Krasnoyarsk, 660022, Russian Federation

Доп.точки доступа:
Volova, T. G.; Shumilova, A. A.; Nikolaeva, E. D.; Kirichenko, A. K.; Shishatskaya, E. I.

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13.

The role of acidosis in the pathogenesis of severe forms of COVID-19 / Y. D. Nechipurenko, D. A. Semyonov, I. A. Lavrinenko [et al.] // Biology. - 2021. - Vol. 10, Is. 9. - Ст. 852, DOI 10.3390/biology10090852 . - ISSN 2079-7737
Кл.слова (ненормированные):
Acidosis -- Bohr effect -- COVID-19 -- Hypoxia -- Lactate -- PH -- SARS-CoV-2 -- Saturation
Аннотация: COVID-19 has specific characteristics that distinguish this disease from many other infec-tions. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient’s condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregu-lation of the immune system and multidirectional pro-and anti-inflammatory reactions, resulting in the development of a “cytokine storm”. In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Laboratory DNA-Protein Recognition, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russian Federation
Institute of Molecular Medicine and Pathobiochemistry, Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, 660022, Russian Federation
Institute of Biophysics Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Department of Human and Animal Physiology, Faculty of Medicine and Biology, Voronezh State University, Voronezh, 394018, Russian Federation
Department of Biological and Medical Physics, Moscow Institute of Physics and Technology, Moscow Region, Dolgoprudny, 141701, Russian Federation
Department of Biophysics, Faculty of Physics, Lomonosov Moscow State University, Moscow, 119991, Russian Federation
Laboratory of Medical Analytical Methods and Devices, Institute for Analytical Instrumentation of the Russian Academy of Sciences, St. Petersburg, 198095, Russian Federation
Sendai Viralytics LLC, Acton, MA 117261, United States
Laboratory of Cellular Bases for the Development of Malignant Diseases, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russian Federation

Доп.точки доступа:
Nechipurenko, Y. D.; Semyonov, D. A.; Lavrinenko, I. A.; Lagutkin, D. A.; Generalov, E. A.; Zaitceva, A. Y.; Matveeva, O. V.; Yegorov, Y. E.

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14.

The Role of Acidosis in the Pathogenesis of Severe Forms of COVID-19 / Y. D. Nechipurenko, D. A. Semyonov, I. A. Lavrinenko [et al.] // Biology-Basel. - 2021. - Vol. 10, Is. 9. - Ст. 852, DOI 10.3390/biology10090852. - Cited References:86. - This research was funded by the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818. . - ISSN 2079-7737

РУБ Biology
Рубрики:
RESPIRATORY-ACIDOSIS
   LACTATE
   COAGULATION
   GLYCOLYSIS
   SECRETION
Кл.слова (ненормированные):
SARS-CoV-2 -- COVID-19 -- acidosis -- hypoxia -- saturation -- Bohr effect -- lactate -- pH
Аннотация: Simple Summary Recently, several studies have shown that acidosis, which is increased acidity in the blood and other body tissues, is often associated with severe COVID-19. In this article, we look at the mechanisms and consequences of acidosis that can lead to an exacerbation of COVID-19. We want to draw the attention of readers to the threshold values of such disease characteristics as hypoxia and acidosis, which are associated with a sharp deterioration in the patient's condition. Hypoxia and acidosis mutually reinforce each other according to the principle of a vicious cycle (that is, they are involved in a system of positive feedbacks). Elevated blood lactate (lactic acid) levels are associated with poor clinical outcomes in COVID patients. As a practical recommendation, we propose to pay more attention to the prevention of acidosis, including in the early stages of the disease, when the adjustment of homeostasis requires less effort and is less risky. COVID-19 has specific characteristics that distinguish this disease from many other infections. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient's condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregulation of the immune system and multidirectional pro- and anti-inflammatory reactions, resulting in the development of a "cytokine storm". In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy.

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Держатели документа:
Russian Acad Sci, Engelhardt Inst Mol Biol, Lab DNA Prot Recognit, Moscow 119991, Russia.
Voyno Yasenetsky Krasnoyarsk State Med Univ, Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Voronezh State Univ, Fac Med & Biol, Dept Human & Anim Physiol, Voronezh 394018, Russia.
Moscow Inst Phys & Technol, Dept Biol & Med Phys, Dolgoprudnyi 141701, Russia.
Lomonosov Moscow State Univ, Fac Phys, Dept Biophys, Moscow 119991, Russia.
Russian Acad Sci, Lab Med Analyt Methods & Devices, Inst Analyt Instrumentat, St Petersburg 198095, Russia.
Sendai Viralyt LLC, Acton, MA USA.
Russian Acad Sci, Engelhardt Inst Mol Biol, Lab Cellular Bases Dev Malignant Dis, Moscow 119991, Russia.

Доп.точки доступа:
Nechipurenko, Yury D.; Semyonov, Denis A.; Lavrinenko, Igor A.; Lagutkin, Denis A.; Generalov, Evgenii A.; Zaitceva, Anna Y.; Matveeva, Olga, V; Yegorov, Yegor E.; Lagutkin, Denis; Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]

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