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In vivo toxicological evaluation of polyhydroxyalkanoates. / E. I. Shishatskaya, T. G. Volova, I. I. Gitelson // Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - P109-111 . - ISSN 0012-4966
Кл.слова (ненормированные):
alkane -- animal -- animal behavior -- article -- cytology -- drug effect -- erythrocyte sedimentation rate -- female -- heart -- kidney -- liver -- lymphoid tissue -- male -- rat -- spleen -- Wistar rat -- Alkanes -- Animals -- Behavior, Animal -- Blood Sedimentation -- Female -- Heart -- Kidney -- Liver -- Lymphoid Tissue -- Male -- Rats -- Rats, Wistar -- Spleen

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gitelson, I.I.

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Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals / E. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - P2185-2203, DOI 10.1163/092050610X537138 . - ISSN 0920-5063
Кл.слова (ненормированные):
14C products of polymer resorption -- intravenous administration -- microparticles -- Resorbable polyhydroxybutyrate -- tissues of internal organs -- 3-hydroxybutyric acid -- sup14/supC products of polymer resorption -- Adverse effect -- Drug-delivery systems -- Growth and development -- High molecular weight -- Initial molecular weight -- Initial values -- Internal organs -- Intravenous administration -- Laboratory animals -- Local response -- matrix -- Mean diameter -- Micro-particles -- Polymer degradation -- Polymer microparticles -- Preparation process -- Radioactivity level -- Residual content -- Resorbable -- Sustained-release -- Tail veins -- Wistar rat -- Animals -- Biocompatibility -- Degradation -- Histology -- Molecular weight -- Morphology -- Radiation -- Radioactivity -- Rats -- Tissue -- poly(3 hydroxybutyric acid) -- animal experiment -- animal tissue -- article -- biocompatibility -- concentration process -- controlled study -- degradation -- drug delivery system -- female -- gel permeation chromatography -- heart -- kidney parenchyma -- liver -- lung parenchyma -- molecular weight -- nonhuman -- organ culture -- priority journal -- radioactivity -- rat -- spleen -- Animals -- Animals, Laboratory -- Biocompatible Materials -- Drug Delivery Systems -- Female -- Infusions, Intravenous -- Materials Testing -- Microspheres -- Molecular Weight -- Particle Size -- Polymers -- Rats -- Rats, Wistar -- Tissue Distribution -- Animalia -- Rattus -- Rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.; Goreva, A.; Kalacheva, G.; Volova, T.

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Dynamics of amino acid composition of the medium during culture of isolated liver and kidneys by the controlled perfusion method / V. A. Barashkov [et al.] // Bulletin of Experimental Biology and Medicine. - 1976. - Vol. 80, Is. 11. - P1305-1307 . - ISSN 0007-4888
Кл.слова (ненормированные):
amino acid -- dog -- in vitro study -- kidney perfusion -- liver perfusion -- theoretical study
Аннотация: The dynamics of the amino acid composition of the medium was investigated during perfusion of the dog liver and kidney for 6 h with a mixture of autogenous plasma and medium No.199 in the ratio of 2:3. During culture of the kidney for 6 h the histidine concentration in the medium increased by 2.2 times compared with initially, the concentration of glutamic acid by 1.7 times, and of alanine and lysine by 1.6 times, whereas the concentrations of arginine, serine, and aspartic acid fell by 3.3 times and those of glutamine with threonine by 2.5 times. During perfusion of the liver the concentration of glutamic acid rose by 2.9 times, of alanine by 2.3 times, cystine by 2.0 times, and glycine by 1.5 times. The concentration of tyrosine fell by half, and that of phenylalanine and serine by 1.4 times. The arginine concentration fell so quickly during perfusion of the liver that by the second hour after the beginning of perfusion no arginine could be found in the medium. The method of amino acid analysis during organ culture as described can be used as a method of developing and correcting culture media.

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Держатели документа:
Siberian Div., Dept. Biophys., L.V. Kirenskii Inst. Phys., Acad. Sci. USSR, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Barashkov, V.A.; Gitel'zon, I.I.; Nefedov, V.P.; Trubachev, I.N.

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Effect of hypoxemia on erythropoietic activity of perfused organs / I. I. Gitelzon, V. P. Nefedov, V. V. Mezhevikin // Byulleten Eksperimentalnoi Biologii i Meditsiny. - 1977. - Vol. 84, Is. 7. - С. 23-24 . - ISSN 0365-9615
Кл.слова (ненормированные):
animal -- bone marrow -- cell proliferation -- erythropoiesis -- histology -- hypoxemia -- injury -- mammal -- perfusion fluid -- theoretical study -- article -- dog -- kidney -- liver -- metabolism -- perfusion -- spleen -- sternum -- erythropoietin -- Animal -- Anoxemia -- Dogs -- English Abstract -- Erythropoietin -- Kidney -- Liver -- Perfusion -- Spleen -- Sternum

Scopus
Держатели документа:
L.V. Kirensky Inst. Phys., Siberian Branch USSR Acad. Sci., Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelzon, I.I.; Nefedov, V.P.; Mezhevikin, V.V.

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The dynamics of the amino acid composition of the medium in cultivating the isolated organs by controlled perfusion (Russian) / V. A. Barashkov [и др.] // Byulleten Eksperimentalnoi Biologii i Meditsiny. - 1975. - Vol. 80, Is. 7. - С. 36-38 . - ISSN 0365-9615
Кл.слова (ненормированные):
amino acid -- amino acid -- dog -- in vitro study -- kidney -- liver -- sternum -- theoretical study -- animal -- article -- culture medium -- metabolism -- organ culture -- organ preservation -- perfusion -- tissue preservation -- kidney -- liver -- kidney perfusion -- liver perfusion -- tissue culture -- Amino Acids -- Animal -- Culture Media -- Dogs -- English Abstract -- In Vitro -- Organ Culture -- Organ Preservation -- Perfusion -- Tissue Preservation -- Kidney -- Liver

Scopus
Держатели документа:
L.V. Kirensky Inst. Phys., Siberian Branch, USSR Acad. Sci., Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Barashkov, V.A.; Gitelzon, I.I.; Nefedov, V.P.; Trubachev, I.N.

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ESTIMATE OF KIDNEY AND LIVER CONTRIBUTIONS TO THE BALANCE OF ERYTHROPOIETIN [Текст] / V. P. NEFEDOV [и др.] // DOKLADY AKADEMII NAUK SSSR. - 1988. - Vol. 301, Is. 5. - С. 1261-1264. - Cited References: 10 . - ISSN 0002-3264

РУБ Multidisciplinary Sciences

: 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
NEFEDOV, V.P.; TASHENOV, E.K.; RUPENKO, A.P.; ALEXEEV, P.V.; GITELZON, I.I.

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EVALUATION OF THE ERYTHROPOIETIN-PRODUCING FUNCTION OF THE KIDNEY AND LIVER DURING CONTROLLED PERFUSION [Text] / V. P. NEFEDOV [et al.] // Bull. Exp. Biol. Med. - 1986. - Vol. 102, Is. 10. - P1340-1342, DOI 10.1007/BF00851040. - Cited References: 14 . - ISSN 0007-4888

РУБ Medicine, Research & Experimental

Держатели документа:
ACAD SCI USSR,INST BIOPHYS,BIOPHYS LAB,SIBERIAN BRANCH,KRASNOYARSK,USSR
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
NEFEDOV, V.P.; MAKAROV, V.P.; TASHENOV, E.K.; KRIZHANOVSKAYA, E.V.; KAZAKOV, V.N.; RUPENKO, A.P.; GITELZON, I.I.

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Oxidative stress monitoring in biological samples by bioluminescent method / N. N. Remmel, N. M. Titova, V. A. Kratasyuk // Bulletin of Experimental Biology and Medicine. - 2003. - Vol. 136, Is. 2. - P. 209-211, DOI 10.1023/A:1026347830283 . - ISSN 0007-4888
Кл.слова (ненормированные):
Bioluminescent analysis -- Lipid peroxidation -- Malonic dialdehyde -- Oxidative stress -- malonaldehyde -- animal tissue -- article -- bioluminescence -- brain tissue -- controlled study -- fluorescence -- freeze drying -- kidney parenchyma -- lipid peroxidation -- liver -- male -- monitoring -- nonhuman -- oxidative stress -- Photobacterium phosphoreum -- rat -- serum -- Animals -- Bacteria -- Biological Assay -- Lipid Peroxidation -- Luminescent Measurements -- Malondialdehyde -- Oxidative Stress -- Photobacterium -- Rats -- Tissue Extracts -- Animalia -- Negibacteria -- Photobacterium -- Photobacterium phosphoreum
Аннотация: The integral bioluminescent biotest with lyophilized fluorescent bacteria was used for monitoring of LPO processes in tissue extracts and serum of rats exposed to stress. A relationship between the content of MDA (LPO indicator) and fluorescence of bacteria was observed in all biological samples.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Remmel, N.N.; Titova, N.M.; Kratasyuk, V.A.

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EVALUATION OF THE ERYTHROPOIETIN-PRODUCING FUNCTION OF THE KIDNEY AND LIVER DURING CONTROLLED PERFUSION [Text] / V. P. NEFEDOV [et al.] // Bull. Exp. Biol. Med. - 1986. - Vol. 102, Is. 10. - P. 1340-1342, DOI 10.1007/BF00851040. - Cited References: 14 . - ISSN 0007-4888

РУБ Medicine, Research & Experimental

WOS
Держатели документа:
ACAD SCI USSR,INST BIOPHYS,BIOPHYS LAB,SIBERIAN BRANCH,KRASNOYARSK,USSR
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
NEFEDOV, V.P.; MAKAROV, V.P.; TASHENOV, E.K.; KRIZHANOVSKAYA, E.V.; KAZAKOV, V.N.; RUPENKO, A.P.; GITELZON, I.I.

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10.

ESTIMATE OF KIDNEY AND LIVER CONTRIBUTIONS TO THE BALANCE OF ERYTHROPOIETIN [Текст] / V. P. NEFEDOV [и др.] // DOKLADY AKADEMII NAUK SSSR. - 1988. - Vol. 301, Is. 5. - P. 1261-1264. - Cited References: 10 . - ISSN 0002-3264

РУБ Multidisciplinary Sciences

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
NEFEDOV, V.P.; TASHENOV, E.K.; RUPENKO, A.P.; ALEXEEV, P.V.; GITELZON, I.I.

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11.

Biodistribution of nanodiamonds in the body of mice using EPR spectrometry / E. Inzhevatkin [et al.] // IET Sci. Meas. Technol. - 2019. - Vol. 13, Is. 7. - P984-988, DOI 10.1049/iet-smt.2018.5594. - Cited References:32. - This work was supported by the Russian Foundation for Basic Research (project no. 16-04-00999). . - ISSN 1751-8822. - ISSN 1751-8830

РУБ Engineering, Electrical & Electronic
Рубрики:
DRUG-DELIVERY
   DETONATION NANODIAMONDS
   NANOMATERIALS
   DOXORUBICIN
Кл.слова (ненормированные):
blood -- biomedical materials -- kidney -- lung -- detonation -- diamond -- nanomedicine -- liver -- muscle -- cellular biophysics -- nanoparticles -- EPR -- imaging -- mice -- EPR spectrometry -- detonation NDs -- electron paramagnetic -- resonance spectrometry -- characteristic EPR signal -- initially injected -- NDs -- detonation -- femoral muscles -- blood -- spleen -- brain -- kidneys -- heart -- lungs -- liver -- biomaterials -- nanodiamonds -- organ homogenates -- nanoparticle concentrations -- inter-organ distribution -- time 2 -- 5 hour -- C
Аннотация: In vitro experiments proved the usefulness of electron paramagnetic resonance (EPR) spectrometry for detecting detonation nanodiamonds (NDs) in samples of biomaterials (blood and homogenates of organs of mice). A characteristic EPR signal (g = 2.003, Delta H similar or equal to 10 G) was detected in biomaterials containing NDs, and its intensity linearly increased at nanoparticle concentrations of between 1.6 and 200 mu g/ml. In vivo experiments demonstrated that EPR spectrometry was effective for monitoring the inter-organ distribution of NDs intravenously injected to mice. In 2.5 h after the injection of NDs, the nanoparticles mainly accumulated in the lungs and liver of the animals - about 25 and 20%, respectively, of the initially injected NDs. The amounts of NDs accumulated in the heart and kidneys were considerably lower. Also, EPR spectrometry did not detect NDs in the blood, spleen, brain, and femoral muscles of mice. Ten days after injection, EPR spectrometry detected redistribution of NDs in mice. The amounts of nanoparticles decreased approximately by a factor of 3.5 in the lungs and increased almost by a factor of 3 in the liver; NDs were detected in the spleen. This study suggests ways to use EPR spectrometry to study the distribution, accumulation, and elimination of detonation NDs injected into laboratory animals.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.
RAS, SB, Int Sci Ctr Studies Extreme States Organism, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.

Доп.точки доступа:
Inzhevatkin, Evgeny; Baron, Alexey; Maksimov, Nikolai; Volkova, Marina; Puzyr, Alexey; Ronzhin, Nikita; Bondar, Vladimir; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [16-04-00999]

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12.

Dietary buckwheat enhances sirtuin1 without calorie restriction / S. Pande, R. Ranjan, A. N. Shuvaev [et al.] // J. Cereal Sci. - 2020. - Vol. 94. - Ст. 103004, DOI 10.1016/j.jcs.2020.103004 . - ISSN 0733-5210
Кл.слова (ненормированные):
Calorie restriction -- Dietary buckwheat -- Male wistar rats -- SIRT1 expression
Аннотация: In the present investigation, the role of dietary intervention in male Wistar rats (n = 8, 3 groups) was studied to observe absolute sirtuin 1 (SIRT1) levels (expressed as ng mg?1 total protein) in serum, stomach, liver, and kidney. Dietary buckwheat at 30% (w/w) level of incorporation in the standard diet (Buckwheat Enriched Diet, BED) improved SIRT1 with values 0.933 ± 0.05, 210 ± 7, 63.26 ± 4, and 69.89 ± 3 in serum, stomach, liver, and kidney respectively when compared to the respective control values of 0.536 ± 0.03, 156 ± 23.3, 31.07 ± 2 and 47.11 ± 4. Moreover, BED though isocaloric to CR diet, led to weight gain (g) by 63.11 ± 3.8, ca. 10%, and 40% higher than control (56.27 ± 5.6) and CR (45.05 ± 4.1) diet groups. A marked rise in Feed Efficiency Ratio (FER) by ca. 37% while a 30% decrease in Feed Conversion Ratio (FCR) was observed for the BED group which supports unexpected weight gain in rats post-dietary intervention. The results justify the superior nutritional profile of buckwheat laden with essential nutrients, essential proteins, and bioactives. In contrast, Calorie Restriction (CR) resulted in a decline of the total protein content in circulation by 19%, while reduction of total protein in stomach, liver, and kidney was estimated to be 95%, 35.2%, and 27% respectively though SIRT1 values were comparatively the highest in all the samples studied. A 30-fold enhancement of SIRT1 in stomach post CR is presumed to counter enhanced stress in gastric tissues. Therefore, mild to moderate expression of SIRT1 may confer beneficial effects such as delayed aging and stress resistance but exceedingly high SIRT1 may evoke increased oxidative stress. © 2020 Elsevier Ltd

Scopus
Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny Prospect 79, Krasnoyarsk, 660041, Russian Federation
Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University Named After Prof. V.F. Voino-Yasenetsky, P. Zheleznyaka 1, Krasnoyarsk, 660022, Russian Federation
Department of Biochemistry, Medical, Pharmaceutical & Toxicological Chemistry, Krasnoyarsk State Medical University Named After Prof. V.F. Voino-Yasenetsky, P. Zheleznyaka 1, Krasnoyarsk, 660022, Russian Federation
Scientific Research Institute of Medical Problems of the North, P. Zheleznyaka 3g, Krasnoyarsk, 660022, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Pande, S.; Ranjan, R.; Shuvaev, A. N.; Malinovskaya, N. A.; Ryazanova, M.; Salmina, A. B.; Kolenchukova, O. A.; Kratasyuk, V. A.

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13.

Dietary buckwheat enhances sirtuin1 without calorie restriction / S. Pande, R. Ranjan, A. N. Shuvaev [et al.] // J. Cereal Sci. - 2020. - Vol. 94. - Ст. 103004, DOI 10.1016/j.jcs.2020.103004. - Cited References:22. - This work was supported by the Russian Foundation for Basic Research (RFBR) [Grant No. 16-06-00-439] and by the Russian Ministry of Education, Post-Doctoral Program of Project "5-100" [Grant No. M 2.2.3]. . - ISSN 0733-5210. - ISSN 1095-9963

РУБ Food Science & Technology
Рубрики:
LIFE-SPAN
   STRESS
   FLOUR
   RATS
Кл.слова (ненормированные):
Dietary buckwheat -- SIRT1 expression -- Calorie restriction -- Male wistar -- rats
Аннотация: In the present investigation, the role of dietary intervention in male Wistar rats (n = 8, 3 groups) was studied to observe absolute sirtuin 1 (SIRT1) levels (expressed as ng mg(-1) total protein) in serum, stomach, liver, and kidney. Dietary buckwheat at 30% (w/w) level of incorporation in the standard diet (Buckwheat Enriched Diet, BED) improved SIRT1 with values 0.933 +/- 0.05, 210 +/- 7, 63.26 +/- 4, and 69.89 +/- 3 in serum, stomach, liver, and kidney respectively when compared to the respective control values of 0.536 +/- 0.03, 156 +/- 23.3, 31.07 +/- 2 and 47.11 +/- 4. Moreover, BED though isocaloric to CR diet, led to weight gain (g) by 63.11 +/- 3.8, ca. 10%, and 40% higher than control (56.27 +/- 5.6) and CR (45.05 +/- 4.1) diet groups. A marked rise in Feed Efficiency Ratio (FER) by ca. 37% while a 30% decrease in Feed Conversion Ratio (FCR) was observed for the BED group which supports unexpected weight gain in rats post-dietary intervention. The results justify the superior nutritional profile of buckwheat laden with essential nutrients, essential proteins, and bioactives. In contrast, Calorie Restriction (CR) resulted in a decline of the total protein content in circulation by 19%, while reduction of total protein in stomach, liver, and kidney was estimated to be 95%, 35.2%, and 27% respectively though SIRT1 values were comparatively the highest in all the samples studied. A 30-fold enhancement of SIRT1 in stomach post CR is presumed to counter enhanced stress in gastric tissues. Therefore, mild to moderate expression of SIRT1 may confer beneficial effects such as delayed aging and stress resistance but exceedingly high SIRT1 may evoke increased oxidative stress.

WOS
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Lab Bioluminescent Biotechnol, Svobodny Prospect 79, Krasnoyarsk 660041, Russia.
Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, P Zheleznyaka 1, Krasnoyarsk 660022, Russia.
Krasnoyarsk State Med Univ, Dept Biochem Med Pharmaceut & Toxicol Chem, P Zheleznyaka 1, Krasnoyarsk 660022, Russia.
Sci Res Inst Med Problems North, P Zheleznyaka 3g, Krasnoyarsk 660022, Russia.
Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Pande, Shubhra; Ranjan, Rajeev; Shuvaev, Anton N.; Malinovskaya, Natalia A.; Ryazanova, Maria; Salmina, Alla B.; Kolenchukova, Oksana A.; Kratasyuk, Valentina A.; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [16-06-00-439]; Russian Ministry of EducationMinistry of Education and Science, Russian Federation [M 2.2.3]

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