Библиотека института биофизики СО РАН

Главная

Базы данных

Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

Каталог диссертаций ИБФ СО РАН

Труды сотрудников ИБФ СО РАН

Область поиска

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>K=live<.>)

Общее количество найденных документов : 14
Показаны документы с 1 по 14

Distribution of Gammarus lacustris Sars (Amphipoda, Gammaridae) in Lake Shira (Khakasia, Siberia) and laboratory study of its growth characteristics / A. Y. Yemelyanova, T. A. Temerova, A. G. Degermendzhy // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P245-256, DOI 10.1023/A:1015624205389 . - ISSN 1386-2588
Кл.слова (ненормированные):
Biomass -- Fresh-water shrimp -- Gammarus lacustris -- Growth -- Numbers -- biomass -- growth rate -- saline lake -- spatial distribution -- zoobenthos -- Russian Federation -- Amphipoda -- cellular organisms -- Decapoda (Crustacea) -- Gammaridae -- Gammarus -- Gammarus lacustris -- Invertebrata
Аннотация: Spatial distribution of Gammarus lacustris in Lake Shira and growth of young specimens that feed on lake biota were studied. The amphipods have been shown to inhabit the littoral, sublittoral and the upper aphytal zones of the lake on stony-sandy soil and silted sand. The young and adult individuals stay apart. The young live in submerged or semi-submerged vegetation in the littoral, the adults in the sublittoral and upper aphytal zones. Maximum density of amphipods was encountered in the areas influenced by human activity. The feeding experiments revealed that the lake plankton is a more important food source for G. lacustris than any other food species. The specific growth rate measured was 0.039 d-1, with a length increment 0.095 mm d-1.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Yemelyanova, A.Y.; Temerova, T.A.; Degermendzhy, A.G.

Найти похожие

Study of non-consumptive mortality of Crustacean zooplankton in a Siberian reservoir using staining for live/dead sorting and sediment traps / O. P. Dubovskaya [et al.] // Hydrobiologia. - 2003. - Vol. 504. - P223-227, DOI 10.1023/B:HYDR.0000008522.88010.45 . - ISSN 0018-8158
Кл.слова (ненормированные):
Cyclops vicinus -- Daphnia -- Live/dead sorting -- Non-consumptive mortality -- Sedimentation -- Reservoirs (water) -- Sampling -- Sediment traps -- Non-consumptive mortality -- Population dynamics -- Biodiversity -- mortality -- population dynamics -- reservoir -- sediment trap -- sedimentation -- zooplankton -- Cladocera -- Copepoda -- Crustacea -- Cyclopia -- Cyclops -- Cyclops vicinus -- Daphnia
Аннотация: We studied non-consumptive (non-predatory) mortality of Daphnia and Cyclops vicinus during four sampling seasons. Mortality estimations were based on live/dead sorting using special staining and measurements of sedimentation rates for dead individuals, depended on wind speed. Original equations were used for calculations. The estimated specific non-consumptive mortality never had biologically senseless negative values, which were often obtained on the basis of the other ways of mortality estimations, and was in a good agreement with other components of population dynamics. As found, the non-consumptive mortality was the important, often the determinant component of the zooplankton population dynamics.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State University, Svobodny av., 79, Krasnoyarsk, 660042, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Dubovskaya, O.P.; Gladyshev, M.I.; Gubanov, V.G.; Makhutova, O.N.

Найти похожие

Evaluation of non-predatory mortality of two Daphnia species in a Siberian reservoir / M. I. Gladyshev [et al.] // Journal of Plankton Research. - 2003. - Vol. 25, Is. 8. - P999-1003, DOI 10.1093/plankt/25.8.999 . - ISSN 0142-7873
Кл.слова (ненормированные):
estimation method -- mortality -- zooplankton -- Russian Federation -- Daphnia -- Daphnia cucullata -- Daphnia longispina
Аннотация: A new method of estimating non-predatory mortality of Zooplankton based on live/dead sorting and sediment trap measurement is described. Preliminary results on Daphnia cucullata and Daphnia longispina are given. Estimations of average non-predatory mortality demonstrated a significant contribution of this kind of mortality to total mortality.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Br. of Russ. Acad. of Sci., Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State University, Svobodny Av, 79, Krasnoyarsk, 660042, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Dubovskaya, O.P.; Gubanov, V.G.; Makhutova, O.N.

Найти похожие

Ecological biophysics and its role in study of aquatic ecosystems / I. I. Gitel'zon [et al.] // Biophysics. - 1993. - Vol. 38, Is. 6. - P1099-1108 . - ISSN 0006-3509
Аннотация: A concept is formulated on ecological and biophysics as a scientific discipline which studies physical processes and phenomena resulting from the functioning of live supra-organism systems. Three main directions of ecological biophysics are defined: creation of methods of monitoring ecosystems on the basis of probing biophysical fields (fields of bioluminescence and fluorescence); mathematical and physical modelling; and study of the role of live organisms in hydrophysical processes on the ecosystem scale. Examples are given of the realization of the ecologo-biophysical approach in research into the ecosystems of the world ocean, Lake Baikal and the Dnieper and Yenisei reservoirs. В© 1994.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitel'zon, I.I.; Gladyshev, M.I.; Degermendzhi, A.G.; Levin, L.A.; Sid'ko, F.Ya.

Найти похожие

Database on natural and transgenic luminous microorganisms: "BIOLUMBASE" / S. E. Medvedeva [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 2. - С. 278-286 . - ISSN 0026-3656
Кл.слова (ненормированные):
Bioluminescence -- Database -- Natural and transgenic luminous microorganisms -- photoprotein -- article -- bacterial gene -- bacterium -- biotechnology -- chemistry -- ecology -- factual database -- genetics -- luminescence -- transgene -- Bacteria -- Biotechnology -- Databases, Factual -- Ecology -- Genes, Bacterial -- Luminescence -- Luminescent Proteins -- Transgenes -- Bacteria (microorganisms)
Аннотация: The database "BiolumBase" is designed for the selection and systematization of available world information on microorganisms containing bioluminescent systems; it includes two sections: "natural" and "transgenic" luminous microorganisms. At present, logic schemes of divisions, classification of the objects, presentation of characteristics, and the inputs of relative information, as well as the necessary program modules including links to the database, are developed. The database is constructed on the basis of published data and our own experimental results; the subsequent linkage of the database to the Internet is envisaged. Users will be able to obtain not only the catalogues of strains but also information concerning the properties and functions of the known species of luminous bacteria, the structure, regulatory mechanisms, and application of bioluminescent systems and genetically engineered constructions with live genes, as well as to find references and to search strains by using any set of attributes. The database will provide information that is of interest for the development of microbial ecology and biotechnology, in particular, for the prediction of biological hazard from the application of transgenic strains.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Boyandin, A.N.; Lankin, Yu.P.; Kotov, D.A.; Kargatova, T.V.; Rodicheva, E.K.; Popova, L.Yu.

Найти похожие

Zooplankton carcasses and non-predatory mortality in freshwater and inland sea environments [Text] / K. W. Tang [et al.] // J. Plankton Res. - 2014. - Vol. 36, Is. 3. - P597-612, DOI 10.1093/plankt/fbu014. - Cited References: 168. - This work was supported by the Humboldt Foundation (Fellowship for Experienced Researchers to K. W. T.); Siberian Branch of Russian Academy of Science (Partner Project No. 8 to M. I. G. and O.P.D.); Federal Tasks of Ministry of Education and Science of Russian Federation (project B-15 of Siberian Federal University to M. I. G.); the Leibniz-Association (SAW-2011-IGB-2 to G. K.); and the German Science foundation (KI-853/7-1 to G. K., GR1540/20-1 to H. P. G.). The manuscript benefited from the constructive comments from three reviewers. . - ISSN 0142-7873. - ISSN 1464-3774

РУБ Marine & Freshwater Biology + Oceanography
Рубрики:
SMALL-SCALE TURBULENCE
   NON-CALANOID COPEPODS
   AGGREGATES LAKE SNOW
   DAPHNIA-GALEATA
   MIDSUMMER DECLINE
   NONCONSUMPTIVE MORTALITY
   CRUSTACEAN ZOOPLANKTON
   CLIMATE-CHANGE
   VERTICAL-DISTRIBUTION
   POPULATION-GROWTH
Кл.слова (ненормированные):
carbon flux -- inland waters -- lakes -- live -- dead sorting -- non-predatory mortality -- zooplankton carcasses
Аннотация: Zooplankton carcasses are ubiquitous in marine and freshwater systems, implicating the importance of non-predatory mortality, but both are often overlooked in ecological studies compared with predatory mortality. The development of several microscopic methods allows the distinction between live and dead zooplankton in field samples, and the reported percentages of dead zooplankton average 11.6 (minimum) to 59.8 (maximum) in marine environments, and 7.4 (minimum) to 47.6 (maximum) in fresh and inland waters. Common causes of non-predatory mortality among zooplankton include senescence, temperature change, physical and chemical stresses, parasitism and food-related factors. Carcasses resulting from non-predatory mortality may undergo decomposition leading to an increase in microbial production and a shift in microbial composition in the water column. Alternatively, sinking carcasses may contribute significantly to vertical carbon flux especially outside the phytoplankton growth seasons, and become a food source for the benthos. Global climate change is already altering freshwater ecosystems on multiple levels, and likely will have significant positive or negative effects on zooplankton non-predatory mortality. Better spatial and temporal studies of zooplankton carcasses and non-predatory mortality rates will improve our understanding of this important but under-appreciated topic.

WOS
Держатели документа:
[Tang, Kam W.] Virginia Inst Marine Sci, Coll William & Mary, Gloucester Point, VA 23062 USA
[Tang, Kam W.] Swansea Univ, Dept Biosci, Swansea SA2 8PP, W Glam, Wales
[Gladyshev, Michail I.
Dubovskaya, Olgo P.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Gladyshev, Michail I.
Dubovskaya, Olgo P.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Kirillin, Georgiy] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Ecohydrol, D-12587 Berlin, Germany
[Grossart, Hans-Peter] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Expt Limmol, D-16775 Stechlin, Germany
[Grossart, Hans-Peter] Univ Potsdam, Inst Biochem & Biol, D-14469 Potsdam, Germany
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tang, K.W.; Gladyshev, M.I.; Dubovskaya, O.P.; Kirillin, G...; Grossart, H.P.; Humboldt Foundation; Ministry of Education and Science of Russian Federation (Siberian Federal University) [B-15]; Leibniz-Association [SAW-2011-IGB-2]; German Science foundation [KI-853/7-1, GR1540/20-1]

Найти похожие

Comparison of fatty acid composition of cladocerans and copepods from lakes of different climatic zones [Text] / O. N. Makhutova [et al.] // Contemp. Probl. Ecol. - 2014. - Vol. 7, Is. 4. - P474-483, DOI 10.1134/S1995425514040076. - Cited References: 47. - This work was supported by Partner Project of the Siberian Branch of the Russian Academy of Sciences no. 8 and Ural Branch of the Russian Academy of Sciences no. 12-C-4-1011; grant from the Russian Foundation for Basic Research no. 11-05-00246-a; grants from "Thematic Plans Program" of the Ministry of Education and Sciences of the Russian Federation (Theme B-9 of Siberian Federal University); and a grant from the Federal Program of the Ministry of Education "Scientific and Scientific-Pedagogical Personnel of an Innovative Russia," national contract no. 16.740.11.0484. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
FRESH-WATER ZOOPLANKTON
   DOCOSAHEXAENOIC ACID
   SEASONAL DYNAMICS
   TROPHIC TRANSFER
   YENISEI RIVER
   LIVE FOOD
   GROWTH
   TEMPERATURE
   SESTON
   RESERVOIR
Кл.слова (ненормированные):
cladocerans -- copepods -- fatty acids -- cold lakes -- warm lakes
Аннотация: We have studied the fatty acid (FA) contents and composition of cladocerans and copepods from warm and cold lakes. We have found no significant differences in FA percent levels and per carbon contents of cladocerans from warm and cold lakes or of copepods from warm and cold lakes. A discriminant analysis showed that all cladocerans differed from all copepods mainly due to the content of docosahexaenoic acid. Compared to cladocerans, copepods had significantly higher levels of all C22 polyunsaturated FA. Thus, we conclude that cladocerans and copepods in all environments had comparatively invariant taxon-specific compositions and contents of long-chain highly unsaturated fatty acids (HUFAs). According to HUFA content, all studied copepods can be regarded as a valuable food for fish.

WOS
Держатели документа:
[Makhutova, O. N.
Gladyshev, M. I.
Sushchik, N. N.
Dubovskaya, O. P.
Kalachova, G. S.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
[Gladyshev, M. I.
Sushchik, N. N.
Dubovskaya, O. P.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Buseva, Z. F.
Semenchenko, V. P.] Natl Acad Sci Belarus Bioresources, Sci & Pract Ctr, Minsk 220072, Byelarus
[Fefilova, E. B.
Kononova, O. N.
Baturina, M. A.] Russian Acad Sci, Komi Sci Ctr, Ural Branch, Inst Biol, Syktyvkar 167982, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Makhutova, O.N.; Gladyshev, M.I.; Sushchik, N.N.; Dubovskaya, O.P.; Buseva, Z.F.; Fefilova, E.B.; Semenchenko, V.P.; Kalachova, G.S.; Kononova, O.N.; Baturina, M.A.; Siberian Branch of the Russian Academy of Sciences; Ural Branch of the Russian Academy of Sciences [12-C-4-1011]; Russian Foundation for Basic Research [11-05-00246-a]; "Thematic Plans Program" of the Ministry of Education and Sciences of the Russian Federation (Siberian Federal University) [B-9]; Federal Program of the Ministry of Education "Scientific and Scientific-Pedagogical Personnel of an Innovative Russia" [16.740.11.0484]

Найти похожие

Study of non-consumptive mortality of Crustacean zooplankton in a Siberian reservoir using staining for live/dead sorting and sediment traps [Text] / O. P. Dubovskaya [et al.] // Hydrobiologia. - 2003. - Vol. 504: 4th International Conference on Reservoir Limnology and Water Quality (AUG, 2002, CESKE BUDEJOVICE, CZECH REPUBLIC), Is. 01.03.2013. - P. 223-227, DOI 10.1023/B:HYDR.0000008522.88010.45. - Cited References: 19 . - ISSN 0018-8158

РУБ Marine & Freshwater Biology
Рубрики:
SEASONAL DYNAMICS
   MIDSUMMER DECLINE
   DAPHNIA
   DETRITUS
   FISH
   POND
Кл.слова (ненормированные):
Cyclops vicinus -- Daphnia -- live/dead sorting -- non-consumptive mortality -- sedimentation
Аннотация: We studied non-consumptive (non-predatory) mortality of Daphnia and Cyclops vicinus during four sampling seasons. Mortality estimations were based on live/dead sorting using special staining and measurements of sedimentation rates for dead individuals, depended on wind speed. Original equations were used for calculations. The estimated specific non-consumptive mortality never had biologically senseless negative values, which were often obtained on the basis of the other ways of mortality estimations, and was in a good agreement with other components of population dynamics. As found, the non-consumptive mortality was the important, often the determinant component of the zooplankton population dynamics.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660042, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Dubovskaya, O.P.; Gladyshev, M.I.; Gubanov, V.G.; Makhutova, O.N.

Найти похожие

Evaluation of non-predatory mortality of two Daphnia species in a Siberian reservoir [Text] / M. I. Gladyshev [et al.] // J. Plankton Res. - 2003. - Vol. 25, Is. 8. - P. 999-1003, DOI 10.1093/plankt/25.8.999. - Cited References: 24 . - ISSN 0142-7873

РУБ Marine & Freshwater Biology + Oceanography
Рубрики:
SEASONAL DYNAMICS
   MIDSUMMER DECLINE
   ZOOPLANKTON
   POND
   POPULATION
   PREDATION
   DETRITUS
   FISH
   LAKE
   TRAP
Аннотация: A new method of estimating non-predatory mortality of zooplankton based on live/dead sorting and sediment trap measurement is described. Preliminary results on Daphnia cucullata and Daphnia longispina are given. Estimations of average non-predatory mortality demonstrated a significant contribution of this kind of mortality to total mortality.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660042, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Dubovskaya, O.P.; Gubanov, V.G.; Makhutova, O.N.

Найти похожие

10.

Biosorption of 241Am from solution and its biochemical fractionation in the mycelium of macromycetes / D. V. Dementyev [et al.] // Radiochemistry. - 2015. - Vol. 57, Is. 6. - P661-665, DOI 10.1134/S1066362215060144 . - ISSN 1066-3622
Кл.слова (ненормированные):
americium -- fungus mycelium -- sorption
Аннотация: Experiments with macromycetes Pleurotus ostreatus, Neonothopanus nambi, and Agaricus bisporus demonstrated for the first time that live mycelium of mushrooms, cultivated on a liquid culture medium, can efficiently take up dissolved 241Am in its biomass. Biochemical fractionation of the mycelium biomass demonstrated for the first time that the major fraction (up to 90%) of 241Am accumulated in the mycelium is bonded to structural polysaccharides of the cell walls. The sorption capacity of cell wall polysaccharides for 241Am is 2.7-3.6 times higher than that of the initial mycelium biomass, and the activity concentration of 241Am in the polysaccharides reached 950 Bq g-1 dry weight. © 2015 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Dementyev, D. V.; Zotina, T. A.; Manukovsky, N. S.; Kalacheva, G. S.

Найти похожие

11.

Dispersal of midges (Chironomidae, Diptera) on terrestrial area after the emergence from saline lake shira / I. A. Vitkovskaya, E. V. Borisova, N. N. Sushchik // J. Siberian Fed. Univ. Humanit. Social Sci. - 2019. - Vol. 12, Is. 2. - С. 216-226, DOI 10.17516/1997-1389-0294 . - ISSN 1997-1370
Кл.слова (ненормированные):
Chironomidae -- Dispersal distance -- Emergence of amphibiotic insects -- Saline lake -- South Siberia
Аннотация: We studied the number of midges (Chironomidae, Diptera) on the terrestrial area where flows of organic matter and essential polyunsaturated fatty acids (PUFA) provided by the emergence of the amphibiotic insects from saline steppe Lake Shira were dispersed. Records of midge specimens captured on sticky traps showed that 50 and 95 percent of the emerged adults of the Glyptotendipes and Polypedilum genera occurred within 25-m and 100-m strips surrounding the lakeshore, respectively. The areas of these strips were calculated along with quantification of flows of chironomid biomass and essential PUFA per land area unit based on data of previously measured export. For the 25-m lake-surrounding strip, where 50% of midges swarmed, the annual import of dry biomass and PUFA accounted for 1.5 g · m-2 and 15 mg · m-2, respectively. Further, the 100-m wide strip, where 95% of the midges were registered, got the annual flows of 0.7 g dry weight · m-2 and 7.2 mg PUFA · m-2. The calculated values of PUFA import were similar to measurements in relatively productive landscapes. The number of midges captured on the site located near the most productive lake part was the lowest among all the studied near-lake sites. This site of the lakeshore is part of a national preserve, where a great number of insectivorous birds live or stop. The lower abundance of swarming chironomids on this site was likely related to intensive consumption by terrestrial animals, i.e., birds, for obtaining necessary doses of essential compounds. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Vitkovskaya, I. A.; Borisova, E. V.; Sushchik, N. N.

Найти похожие

12.

Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system / D. Chang, Y. K. Liu, Y. Y. Chen [et al.] // BMC Vet. Res. - 2020. - Vol. 16, Is. 1. - Ст. 202, DOI 10.1186/s12917-020-02422-3. - Cited References:30. - This work was financially supported by the National Natural Science Foundation of China (No. 31870917), The program for Innovative Research Team of Science and Technology in University of Henan Province (No. 20IRTSTHN024) and Key Scientific Research Projects of Colleges and Universities in Henan Province of China (No. 18B230008). The funding bodies played no role in the design of the study, the collection, analysis, and interpretation of data and in writing the manuscript. . - ISSN 1746-6148

РУБ Veterinary Sciences
Рубрики:
VIRUS-LIKE PARTICLES
   ESCHERICHIA-COLI
   GENETIC-ANALYSIS
   CPV-VP2
Кл.слова (ненормированные):
Canine parvovirus -- VP2 protein -- Baculovirus expression system
Аннотация: Background Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice. Results The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expression system. The recombinant virus carrying two copies of VP2 gene showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein reacted with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody. BALB/c mice were intramuscularly immunized with purified VP2 protein twice at 2 week intervals. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions Full length CPV VP2 protein was expressed at high level and purified efficiently. Moreover, it stimulated mice to produce high level of antibodies with hemmaglutination inhibition properties. The VP2 protein expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection.

WOS
Держатели документа:
Nanyang Normal Univ, Sch Agr Engn, Henan Provincal Engn & Technol Ctr Hlth Prod Live, Nanyang 473061, Peoples R China.
Nanyang Normal Univ, Sch Agr Engn, Key Lab Ecol Secur, Nanyang 473061, Peoples R China.
Nanyang Normal Univ, Sch Agr Engn, Collaborat Innovat Ctr Water Secur Water Source R, Nanyang 473061, Peoples R China.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Inst Biophys,Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Chang, Dao; Liu, Yangkun; Chen, Yangyang; Hu, Xiaomin; Burov, Andrey; Puzyr, Alexey; Bondar, Vladimir; Yao, Lunguang; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31870917]; program for Innovative Research Team of Science and Technology in University of Henan Province [20IRTSTHN024]; Key Scientific Research Projects of Colleges and Universities in Henan Province of China [18B230008]

Найти похожие

13.

Morphological and Genetic Diversity of Two Forms of Muksun Coregonus muksun (Salmonidae) of the Khatanga River Basin as a Key for Understanding the Phylogenetic Relationships between Muksun and Whitefish C. lavaretus / E. A. Borovikova, Y. V. Budin // J. Ichthyol. - 2020. - Vol. 60, Is. 6. - P909-922, DOI 10.1134/S0032945220060016 . - ISSN 0032-9452
Кл.слова (ненормированные):
high-density-rakered form -- ITS1 -- Khatanga River -- low-density-rakered form -- mitochondrial DNA -- morphology -- muksun Coregonus muksun -- phylogeny
Аннотация: Abstract: Morphological analysis of muksun Coregonus muksun of the Khatanga River basin revealed that along with its typical high-density-rakered form, individuals of the low-density-rakered form also live in this region. Analysis of the genetic polymorphism of three marker regions of mitochondrial (ND1 and COI fragments) and nuclear (ITS1) DNA indicates the polyphyletic origin of these forms. The revealed low level of genetic differentiation of muksun and whitefish C. lavaretus, along with the available data on the absence of clear diagnostic species characteristics, suggest that they belong to the same biological species C. lavaretus. © 2020, The Author(s).

Scopus
Держатели документа:
Papanin Institute for Biology of Inland Waters—IBIW RAS, Borok, Russian Federation
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences—IBP RAS, Krasnoyarsk, Russian Federation
Krasnoyarsk Branch of All-Russian Scientific Research Institute of Fisheries and Oceanography, Krasnoyarsk, Russian Federation
Krasnoyarsk State Agrarian University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Borovikova, E. A.; Budin, Y. V.

Найти похожие

14.

РУБ Fisheries + Zoology
Рубрики:
PHYLOGEOGRAPHY
   POPULATION
   ALBULA
   IDENTIFICATION
   PLESHCHEYEVO
   MTDNA
Кл.слова (ненормированные):
muksun Coregonus muksun -- low-density-rakered form -- high-density-rakered -- form -- morphology -- mitochondrial DNA -- ITS1 -- phylogeny -- Khatanga River
Аннотация: Morphological analysis of muksun Coregonus muksun of the Khatanga River basin revealed that along with its typical high-density-rakered form, individuals of the low-density-rakered form also live in this region. Analysis of the genetic polymorphism of three marker regions of mitochondrial (ND1 and COI fragments) and nuclear (ITS1) DNA indicates the polyphyletic origin of these forms. The revealed low level of genetic differentiation of muksun and whitefish C. lavaretus, along with the available data on the absence of clear diagnostic species characteristics, suggest that they belong to the same biological species C. lavaretus.

WOS
Держатели документа:
RAS, IBIW, Papanin Inst Biol Inland Waters, Borok, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, IBP,RAS, Krasnoyarsk, Russia.
All Russian Sci Res Inst Fisheries & Oceanog, Krasnoyarsk Branch, Krasnoyarsk, Russia.
Krasnoyarsk State Agr Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Borovikova, E. A.; Budin, Yu. V.; Russian Scientific FoundationRussian Science Foundation (RSF) [16-14-10001]

Найти похожие

Тематический навигатор

Другие библиотеки

Библиотека института физики

Центральная научная библиотека КНЦ СО РАН

Библиотека института химии и химический технологии

Библиотека института вычислительного моделирования

Библиотека института леса

Библиотека СФУ

Краевая научная библиотека

© Международная Ассоциация пользователей и разработчиков электронных библиотек и новых информационных технологий
(Ассоциация ЭБНИТ)