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Accumulation and release of 99Tc by a macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments / A. Bolsunovsky, L. Bondareva // Journal of Radioanalytical and Nuclear Chemistry. - 2008. - Vol. 277, Is. 3. - P631-636, DOI 10.1007/s10967-007-7148-5 . - ISSN 0236-5731
Кл.слова (ненормированные):
technetium 99m -- aquatic flora -- article -- biomass -- controlled study -- dry weight -- liquid scintillation counting -- macrophyte -- nonhuman -- radiation absorption -- radiation detection -- radiation dose fractionation -- radiation measurement -- radioactivity -- river -- water sampling
Аннотация: The study addresses 99Tc accumulation and release by Elodea canadensis, one of the abundant species of submerged plants in the Yenisei River. 99Tc in water samples of the "Elodea - Yenisei River water" model system and in the biomass fractions was measured using a liquid scintillation analyzer. Experiments on accumulation of 99Tc by Elodea showed that 99Tc activity concentration can reach 120В±6 Bq/g dry wt, with the concentration factor for 99Tc 2700В±500 l/kg dry wt. In experiments on 99Tc release, over 504 hours about 82% of the total 99Tc activity was released into the water from the plant; most of 99Tc was released within the first 192 hours. The data obtained using sequential chemical fractionation of biomass confirmed the experimental data on 99Tc release, which suggested that most of the biomass-bound 99Tc was adsorbed on the surface of Elodea. 99Tc tightly bound to biomass (fractions of organics and mineral residue) constituted just 17% of the total 99Tc activity. В© 2008 Akademiai Kiado, Budapest.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Bondareva, L.

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A one-dimensional model of vertical stratification of Lake Shira focussed on winter conditions and ice cover / S. N. Genova [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P571-584, DOI 10.1007/s10452-010-9327-7 . - ISSN 1386-2588
Кл.слова (ненормированные):
Convective mixed layer -- Ice formation -- Ice melting -- Simplified model -- Winter profiles -- anoxic conditions -- hydrogen sulfide -- ice cover -- inhomogeneity -- meromictic lake -- numerical model -- one-dimensional modeling -- pycnocline -- salinity -- seasonality -- stratification -- vertical profile -- water column -- water temperature -- Khakassia -- Lake Shira -- Russian Federation
Аннотация: In meromictic lakes such as Lake Shira, horizontal inhomogeneity is small in comparison with vertical gradients. To determine the vertical distribution of temperature, salinity, and density of water in a deep zone of a Lake Shira, or other saline lakes, a one-dimensional (in vertical direction) mathematical model is presented. A special feature of this model is that it takes into account the process of ice formation. The model of ice formation is based on the one-phase Stefan problem with the linear temperature distribution in the solid phase. A convective mixed layer is formed under an ice cover due to salt extraction in the ice formation process. To obtain analytical solutions for the vertical distribution of temperature, salinity, and density of water, we use a scheme of vertical structure in the form of several layers. In spring, the ice melts as top and bottom. These processes are taken into account in the model. The calculated profiles of salinity and temperature of Shira Lake are in good agreement with field measurement data for each season. Additionally, we focussed on the redox zone, which is the zone in which the aerobic layers of a water column meet the anaerobic ones. Hyperactivity of plankton communities is observed in this zone in lakes with hydrogen sulphide monimolimnion, and Lake Shira is among them. The location of the redox zone in the lake, which is estimated from field measurements, coincides with a sharp increase in density (the pycnocline) during autumn and winter. During spring and summer, the redox zone is deeper than the pycnocline. The location of pycnocline calculated with the hydro physical model is in good agreement with field measurement data. В© 2010 Springer Science+Business Media B.V.

Scopus
Держатели документа:
Institute of Computational Modeling SB RAS, Akademgorodok 50/44, Krasnoyarsk 660036, Russian Federation
Krasnoyarsk, Siberian Federal University, Institute of Mathematics, Svobodny 79, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Federal University, Institute of Mathematics, Svobodny 79, Krasnoyarsk 660041, Russian Federation
Netherlands Institute of Ecology, Center for Limnology, Rijksstraatweg 6, Nieuwersluis 3631 AC, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Genova, S.N.; Belolipetskii, V.M.; Rogozin, D.Y.; Degermendzhy, A.G.; Mooij, W.M.

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A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation / E. I. Shishatskaya, I. A. Khlusov, T. G. Volova // Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - P481-498, DOI 10.1163/156856206776986242 . - ISSN 0920-5063
Кл.слова (ненормированные):
Biocompatibility -- Hydroxyapatite (HA) -- PHB-hydroxyapatite composite -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (P(3HB)) -- Properties -- Biocompatibility -- Differential thermal analysis -- Electron microscopy -- Free energy -- Interfacial energy -- Physical properties -- Surface properties -- X ray analysis -- Biomedical application -- Physicochemical properties -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (PHB) -- Hydroxyapatite -- hydroxyapatite -- poly(3 hydroxybutyric acid) -- polymer -- biomaterial -- hydroxybutyric acid -- adhesion -- animal cell -- animal tissue -- article -- biomedicine -- bone marrow cell -- cell differentiation -- cell growth -- chemical structure -- composite material -- controlled study -- crystallization -- decomposition -- electron microscopy -- in vitro study -- in vivo study -- melting point -- mouse -- nonhuman -- ossification -- osteoblast -- physical chemistry -- priority journal -- rat -- strength -- structure analysis -- surface property -- synthesis -- temperature measurement -- thermal analysis -- tissue engineering -- wettability -- animal -- biomechanics -- bioremediation -- bone prosthesis -- cattle -- cell culture -- chemistry -- cytology -- differential scanning calorimetry -- drug effect -- human -- materials testing -- prostheses and orthoses -- scanning electron microscopy -- standard -- Wistar rat -- Murinae -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Biomechanics -- Bone Substitutes -- Cattle -- Cells, Cultured -- Differential Thermal Analysis -- Durapatite -- Humans -- Hydroxybutyrates -- Materials Testing -- Microscopy, Electron, Scanning -- Osteoblasts -- Prostheses and Implants -- Rats -- Rats, Wistar -- Surface Properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Tomsk State University, Tomsk 634021, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Khlusov, I.A.; Volova, T.G.

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Tritium in surface waters of the Yenisei River basin / A. Y. Bolsunovsky, L. G. Bondareva // Journal of Environmental Radioactivity. - 2003. - Vol. 66, Is. 3. - P285-294, DOI 10.1016/S0265-931X(02)00132-7 . - ISSN 0265-931X
Кл.слова (ненормированные):
Deep-well injection of radionuclides -- Nuclear reactor -- Surface and ground waters -- Tritium -- Yenisei River basin -- Cooling water -- Nuclear reactors -- Sediments -- Surface waters -- River basins -- Tritium -- carbon 14 -- surface water -- tritium -- groundwater -- nuclear power plant -- radioactive pollution -- surface water -- tritium -- article -- catchment -- measurement -- radioactive contamination -- river -- sediment -- Environmental Monitoring -- Geologic Sediments -- Mining -- Power Plants -- Reference Values -- Russia -- Tritium -- Water Pollutants, Radioactive -- Russian Federation -- Tritium
Аннотация: This paper reports an investigation of the tritium content in the surface waters of the Yenisei River basin near the Mining and Chemical Combine (MCC). In 2001 the maximum tritium concentration in the Yenisei River did not exceed 4 В± 1 Bq l-1, which is consistent with the data of 1998-99. However, it has been found that there are surface waters containing enhanced tritium as compared with the background values for the Yenisei River. For instance, in the Ploskii Stream and the Shumikha River the maximum tritium concentrations amount to 168 and 81 Bq l-1, respectively. The source of tritium in these surface waters is the last operating reactor at the MCC, which still uses the Yenisei water as coolant. In water and sediment samples of the Bolshaya Tel River (a tributary of the Yenisei River) the tritium content turned out to be at least 10 times higher than the background values for the Yenisei River. The measurements conducted at the RPA RADON (Moscow) revealed not only tritium but also the artificial radionuclide 14C in the Bolshaya Tel samples. The data obtained suggest that the Bolshaya Tel River receives the major part of tritium from sediments rather than from the water catchment area. This allows the conclusion that there is water exchange between the surface waters and the radioactively contaminated underground horizons of the "Severny" testing site. В© 2002 Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.Y.; Bondareva, L.G.

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Evaluation of non-predatory mortality of two Daphnia species in a Siberian reservoir / M. I. Gladyshev [et al.] // Journal of Plankton Research. - 2003. - Vol. 25, Is. 8. - P999-1003, DOI 10.1093/plankt/25.8.999 . - ISSN 0142-7873
Кл.слова (ненормированные):
estimation method -- mortality -- zooplankton -- Russian Federation -- Daphnia -- Daphnia cucullata -- Daphnia longispina
Аннотация: A new method of estimating non-predatory mortality of Zooplankton based on live/dead sorting and sediment trap measurement is described. Preliminary results on Daphnia cucullata and Daphnia longispina are given. Estimations of average non-predatory mortality demonstrated a significant contribution of this kind of mortality to total mortality.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Br. of Russ. Acad. of Sci., Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State University, Svobodny Av, 79, Krasnoyarsk, 660042, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Dubovskaya, O.P.; Gubanov, V.G.; Makhutova, O.N.

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Measurement of chlorophyll in surface waters from an airplane / I. A. Terskov, I. I. Gitel'zon, A. P. Shevyrnogov // Doklady Biophysics. - 1976. - Vol. Vol.226-228. - P30-33 . - ISSN 0012-4974
Кл.слова (ненормированные):
chlorophyll -- methodology

Scopus
Держатели документа:
L.V. Kirenskii Inst. Phys., Acad. Sci. USSR, Siberian Branch, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Terskov, I.A.; Gitel'zon, I.I.; Shevyrnogov, A.P.

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A study of forest vegetation dynamics in the south of the Krasnoyarskii Krai in spring / M. Chernetskiy [et al.] // Advances in Space Research. - 2011. - Vol. 48, Is. 5. - P819-825, DOI 10.1016/j.asr.2011.04.032 . - ISSN 0273-1177
Кл.слова (ненормированные):
EVI -- Forestry -- MODIS -- NDVI -- Remote sensing -- Vegetation phenology -- Accurate measurement -- Annual time series -- Carbon exchange -- Data series -- Dynamic state -- Enhanced vegetation index -- EVI -- Forest vegetation -- Global scale -- Growth dynamics -- Interannual variability -- Moderate resolution imaging spectroradiometer -- MODIS -- NDVI -- Normalized difference vegetation index -- Principal components analysis -- Remote sensing applications -- Remote sensing data -- Satellite data -- Spatial structure -- Spring season -- Terrestrial ecosystems -- Vegetation dynamics -- Vegetation phenology -- Biology -- Climate models -- Dynamics -- Ecosystems -- Estimation -- Forestry -- Monitoring -- Principal component analysis -- Radiometers -- Remote sensing -- Satellite imagery -- Timber -- Time series -- User interfaces -- Vegetation -- Carbon -- Ecosystems -- Forests -- Image Analysis -- Plants -- Remote Sensing -- Time Series Analysis
Аннотация: Remote sensing applications have greatly enhanced ability to monitor and manage in the areas of forestry. Accurate measurements of regional and global scale vegetation dynamics (phenology) are required to improve models and understanding of inter-annual variability in terrestrial ecosystem carbon exchange and climate-biosphere interactions. Study of vegetation phenology is required for understanding of variability in ecosystem. In this paper, monitoring of vegetation dynamics using time series of satellite data is presented. Vegetation variability (vegetation rate) in different topoclimatic areas is investigated. Original software using IDL interactive language for processing of satellite long-term data series was developed. To investigate growth dynamics vegetation rate inferred from remote sensing was used. All estimations based on annual time series of Moderate Resolution Imaging Spectroradiometer (MODIS) imagery. Vegetation rate for Enhanced Vegetation Index (EVI) and Normalized Difference Vegetation Index (NDVI) was calculated using MODIS data. The time series covers spring seasons of each of 9 years, from 2000 to 2008. Comparison of EVI and NDVI derived growth rates has shown that NDVI derived rates reveal spatial structure better. Using long-term data of vegetation rates variance was estimated that helps to reveal areas with anomalous growth rate. Such estimation shows sensitivity degree of different areas to different topoclimatic conditions. Woods of heights depend on spatial topoclimatic variability unlike woods of lowlands. Principal components analysis shows vegetation with different rate conditions. Also it reveals vegetation of same type in areas with different conditions. It was demonstrated that using of methods for estimating the dynamic state of vegetation based on remote sensing data enables successful monitoring of vegetation phenology. В© 2011 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Chernetskiy, M.; Pasko, I.; Shevyrnogov, A.; Slyusar, N.; Khodyayev, A.

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Spatial and temporal anomalies of sea surface temperature in global scale (by space-based data) / A. Shevyrnogov, G. Vysotskaya, E. Shevyrnogov // Advances in Space Research. - 2004. - Vol. 33, Is. 7. - P1179-1183, DOI 10.1016/S0273-1177(03)00369-7 . - ISSN 0273-1177
Кл.слова (ненормированные):
Anomalies -- Chlorophyll -- Hydrological structure -- Space-based data -- Temperature -- Chlorophyll -- Data reduction -- Oceanography -- Satellites -- Surface properties -- Temperature measurement -- Satellite images -- Spatial distribution -- Space research -- AVHRR -- chlorophyll -- remote sensing -- satellite imagery -- sea surface temperature
Аннотация: The work presents the data on the spatial distribution of temperature anomalies in the Global Ocean, based on the long-term measurements with the AVHRR satellite equipment. It is noteworthy that such results can be only obtained by using long time series of satellite images. To obtain them by ship-borne methods is actually impossible. The proposed anomaly criterion is equal to a difference between minimum/maximum and average temperature, normed for the mean square deviation. The difference is calculated from the whole time series (with regard to a season). Based on this criterion, anomalies can be distinguished on the background of usual seasonal dynamics at various absolute values of deviations of the measured parameter. Besides, rare, single, fluctuations can be also revealed. Based on the proposed criterion, connected zones have been defined. These zones occur almost in every part of the Global Ocean. The data obtained have been analyzed in association with the CZCS (1978-1986) and SeaWiFS satellite images in order to compare them with the areas of anomalous dynamics of chlorophyll concentration and the regions quasistationary in the seasonal dynamics of chlorophyll. Anomalies in the temperature dynamics are of exceptional importance for the formation of climatic deviations; they affect the ocean-atmosphere interaction and reflect variations in the hydrological structure of the surface waters. The work presents a comparison with the dynamics of chlorophyll concentration distribution determined from the SeaWiFS data for the Atlantic Ocean. В© 2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk, Akademgorodok 660036, Russian Federation
Inst Comp Modeling of SB RAS, Krasnoyarsk, Akademgorodok 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.; Vysotskaya, G.; Shevyrnogov, E.

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Results of analysis of spatial and temporal long-term stability of quasistationary areas in the Atlantic Ocean based on multi-satellite measurements (CZCS and SeaWiFS data) / A. Shevyrnogov, G. Vysotskaya, E. Shevyrnogov // Advances in Space Research. - 2004. - Vol. 33, Is. 7. - P1184-1188, DOI 10.1016/S0273-1177(03)00366-1 . - ISSN 0273-1177
Кл.слова (ненормированные):
Chlorophyll -- Hydrological structure -- Seasonal dynamics -- Space-based data -- Chlorophyll -- Data reduction -- Hydrology -- Oceanography -- Satellites -- Quasistationary -- Spatial distribution -- Space research -- chlorophyll -- CZCS -- oceanic circulation -- remote sensing -- SeaWiFS -- spatiotemporal analysis -- Atlantic Ocean
Аннотация: The paper analyzes the stability of areas in the Global Ocean, which are quasistationary with respect to the seasonal dynamics of chlorophyll concentration. Such areas were first discovered based on the CZCS data. They occur almost in all regions of the Global Ocean. They reflect typical seasonal dynamics of phytoplankton, which depends on both large ocean currents and local hydrological instability. It is related to the displacement of frontal zones and to local hydrological anomalies. Since the completion of the CZCS work there has been a 10 years gap in monitoring chlorophyll spatial distribution dynamics in the ocean. The reason is the long absence of equipment on the orbit, which would be suitable for the measurement of chlorophyll concentration. Recently, SeaWiFS data have been accumulated and their quantity is sufficient for the crude statistical analysis of image time series for 25 years. Thus, it has become possible to obtain data on the long-term stability of quasistationary areas and, therefore, on the stability of large-scale hydrological phenomena in the Atlantic Ocean. В© 2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Inst Comp Modeling of SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.; Vysotskaya, G.; Shevyrnogov, E.

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10.

Effect of NaCl concentration on productivity and mineral composition of Salicornia europaea as a potential crop for utilization NaCl in LSS / S. A. Ushakova [et al.] // Advances in Space Research. - 2005. - Vol. 36, Is. 7. - P1349-1353, DOI 10.1016/j.asr.2004.09.017 . - ISSN 0273-1177
Кл.слова (ненормированные):
Life support system -- NaCl -- Salicornia europaea -- Space biology -- Calcium -- Concentration (process) -- Minerals -- Photosynthesis -- Plants (botany) -- Salinity measurement -- Sodium chloride -- Vegetation -- Life support systems -- Liquid wastes -- NaCl -- Salicornea europea -- Space biology -- Space research
Аннотация: The accumulation of solid and liquid wastes in manmade ecosystems presents a problem that has not been efficiently solved yet. Urine, containing NaCl, are part of these products. This is an obstacle to the creation of biological systems with a largely closed material cycling, because the amount of solid and liquid wastes in them must be reduced to a minimum. A possible solution to the problem is to select plant species capable of utilizing sufficiently high concentrations of NaCl, edible for humans, and featuring high productivity. Until recently, the life support systems have included the higher plants that were either sensitive to salinization (wheat, many of the legumes, carrot, potato, maize) or relatively salt-resistant (barley, sugar beet, spinach). Salicomia europaea, whose above-ground part is fully edible for humans, is one of the most promising candidates to be included in life support systems. It is reported in the literature that this plant is capable of accumulating up to 50% NaCl (dry basis). Besides, excessive accumulation of sodium ions should bring forth a decrease in the uptake of potassium ions and other biogenic elements. The aim of this work is to study the feasibility of using S. europaea plants in growth chambers to involve NaCl into material cycling. Plants were grown in vegetation chambers at the irradiance of 100 or 150 W/m2 PAR (photosynthetically active radiation) and the air temperature 24 В°C, by two methods. The first method was to grow the plants on substrate - peat. The peat was supplemented with either 3% NaCl (Variant 1) or 6% NaCl (Variant 2) of the oven-dry mass of the peat. The second method was to grow the plants in water culture, using the solution with a full complement of nutrients, which contained 0.0005% of NaCl, 1% or 2%. The study showed that the addition of NaCl to the substrate or to the solution resulted in the formation of more succulent plants, which considerably increased their biomass. The amount of NaCl uptake was the highest in the plants grown in water culture, 2.6 g per plant. As the sodium uptake increased, the consumption of potassium and the sum of the reduced N forms decreased twofold. The uptake of calcium and magnesium by plants decreased as the NaCl concentration increased; the smallest amounts were taken up by S. europaea grown in water culture. Salinity had practically no effect on the uptake of phosphorus and sulfur. Thus, S. europaea is a promising candidate to be included in life support systems; of special interest is further research on growing these plants in water culture. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Science, Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Kovaleva, N.P.; Gribovskaya, I.V.; Dolgushev, V.A.; Tikhomirova, N.A.

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11.

Closed artificial ecosystems as a means of ecosystem studies for earth and space needs / N. S. Pechurkin, I. M. Shirobokova // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1497-1504, DOI 10.1016/S0273-1177(01)00244-7 . - ISSN 0273-1177
Кл.слова (ненормированные):
artificial ecosystem -- bioremediation -- biosphere -- ecosystem -- environmental monitoring -- model -- Bioremediation -- Ecology -- Ecosystems -- Health -- Biosphere -- Space research -- artificial ecosystem -- Biodegradation, Environmental -- Earth (Planet) -- Ecological Systems, Closed -- Ecology -- Ecosystem -- Energy Transfer -- Environmental Microbiology -- Life Support Systems -- Population Dynamics -- Yeasts
Аннотация: Closed Artificial ecosystems (CAES) have good prospects for wide use as new means for quantitative studies of different types of both natural ecosystems and man-made ones. The paper deals with the discussion of three points of CAES applications. The first one is of importance for theoretical ecology development and is connected with bringing together В«holisticВ» and В«merologicalВ» approaches in ecosystems studies. Using CAES, we can combine both approaches, taking into account the biotic turnover of limiting substrates which few in number even for complicated natural ecosystems. The second CAES use concerns the development of В«ecosystems healthВ» concept and application of a key-factor-approach for the indication and measurement of healthy unhealthy state and functioning of ecosystems or their links. The third use is more of an applied nature, oriented to the intensification of bioremediation or biodepollution processes in different types of ecosystems, including the global biosphere. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Shirobokova, I.M.

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12.

Bioluminescence in oceanology. / I. I. Gitelson, L. A. Levin // Journal of bioluminescence and chemiluminescence. - 1989. - Vol. 4, Is. 1. - P555-562 . - ISSN 0884-3996
Кл.слова (ненормированные):
article -- biology -- circadian rhythm -- ecology -- gas -- instrumentation -- luminescence -- oceanography -- Circadian Rhythm -- Ecology -- Gases -- Luminescence -- Marine Biology -- Oceanography
Аннотация: For analytical purposes bioluminescence can be used in three main ways: 1. luminescence measurement of bioluminescent system components isolated in vitro; 2. determination of luminous organisms' reaction to the in vivo test-action; 3. measurement of bioluminescence in marine ecological systems. The majority of the reports of this Symposium are dealing with the first two topics. The aim of our presentation is to draw attention to the third one. The possibilities of bioluminescent analysis are wider than its traditional scheme of applications in the laboratory, when the emitting system is withdrawn from a native source and is placed in a cuvette of the light measuring device. The reverse scheme is also possible, i.e. the device can be introduced into light emitting system such as a marine biocenosis--the community of the sea inhabitants--where we obtain a highly sensitive and rapid means of gaining the information on the vital activity of marine ecosystems, i.e. their spatial structure, rhythms, man's influence upon them, etc. The present communication will consider the possibilities of this form of bioluminescent analysis.

Scopus
Держатели документа:
Institute of Biophysics, USSR Academy of Sciences, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Levin, L.A.

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13.

Ca2+-regulated photoproteins: Structural insight into the bioluminescence mechanism [Text] / E. S. Vysotski, J. . Lee // Accounts Chem. Res. - 2004. - Vol. 37, Is. 6. - P405-415, DOI 10.1021/ar0400037. - Cited References: 44 . - ISSN 0001-4842

РУБ Chemistry, Multidisciplinary
Рубрики:
AEQUORIN BIOLUMINESCENCE
   SEQUENCE-ANALYSIS
   CALCIUM-BINDING
   CA2+-BINDING PHOTOPROTEIN
   VIOLET BIOLUMINESCENCE
   ANGSTROM RESOLUTION
   FUNCTIONAL PART
   EXCITED-STATES
   W92F OBELIN
   COELENTERAZINE
Аннотация: The bioluminescent jellyfish has contributed two famous proteins to modern science: green fluorescent protein or GFP, which finds wide use as a probe in cell biology studies, and aequorin, which has been used for intracellular calcium measurement for more than 30 years. More recently, obelin, a protein from the bioluminescent hydroid and also in the family of what are called "Ca2+-regulated photoproteins", has been shown to have very attractive properties both in general applications and for basic structural biology investigations. This review will survey the new information into their molecular mechanism of bioluminescence action.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vysotski, E.S.; Lee, J...

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14.

OBELIN MESSENGER-RNA - A NEW TOOL FOR STUDIES OF TRANSLATION IN CELL-FREE SYSTEMS [Text] / S. V. MATVEEV [et al.] // Anal. Biochem. - 1995. - Vol. 231, Is. 1. - P34-39, DOI 10.1006/abio.1995.1499. - Cited References: 17 . - ISSN 0003-2697

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Chemistry, Analytical
Рубрики:
MESSENGER-RNA
   AEQUORIN
   PROTEIN
   CLONING
   CDNA
Аннотация: Obelin mRNA obtained in vitro with the aid of SP6 RNA polymerase was translated in a wheat germ cell-free system, Only the polypeptide with a molecular mass of about 20 kDa was synthesized. The activation of apoobelin with a synthetic coelenterazine revealed a luminescence activity initiated by calcium. The specific activity was 3.6 +/- 0.4 x 10(15) photons per mg of the in vitro synthesized obelin (k = 6.9 s(-1)). The luminescence of the obelin was in a good correlation with the protein concentration calculated by the incorporation of [C-14]Leu. The determination of the amount of de novo synthesized obelin based on measurement of its luminescence is one-thousand times more sensitive than the approach based on the incorporation of labeled amino acid. Thus, obelin mRNA has some advantages for evaluating the efficiency of cell-free translation when compared with standard methods. (C) 1995 Academic Press, Inc.

Держатели документа:
RUSSIAN ACAD SCI,INST BIOPHYS,KRASNOYARSK 660036,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
MATVEEV, S.V.; ILLARIONOV, B.A.; VYSOTSKI, E.S.; BONDAR, V.S.; MARKOVA, S.V.; ALAKHOV, Y.B.

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15.

Bioluminescent signal system: bioluminescence immunoassay of pathogenic organisms [Text] / L. . Frank [et al.] // Luminescence. - 2007. - Vol. 22, Is. 3. - P215-220, DOI 10.1002/bio.952. - Cited References: 14 . - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology
Рубрики:
AEQUORIN
   AGENTS
   OBELIN
   ASSAYS
   LABEL
Кл.слова (ненормированные):
obelin -- bioluminescence immunoassay -- infective agents
Аннотация: The Ca2+-regulated photoprotein obelin has been examined as a label for bioluminescence immunoassay of infective agents. The hepatitis B virus (HbsAg) and the bacteria Escherichia coli and Shigella sonnei lipopolysaccharide (LPS) were chosen as model antigens. Chemically synthesized obelin-corresponding antibody conjugates were used in a solid-phase microplate immunoassay. The sensitivities achieved by the assay were 0.25 ng/mL for S. sonnei LPS and 0.375 ng/mL for HbsAg. A novel, filter-based immunoassay to determine bacterial admixtures in the environment was proposed. The NanoCeram filters were effectively applied to 'trap' and pre-concentrate pathogens from samples under study for the purposes of further detection and measurement of the absorbed material by bioluminescence immunoassay. Copyright (C) 2007 John Wiley & Sons, Ltd.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L...; Markova, S...; Remmel, N...; Vysotski, E...; Gitelson, I...

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16.

Direct measurement of excitation transfer in the protein complex of bacterial luciferase hydroxyflavin and the associated yellow fluorescence proteins from Vibrio fischeri Y1 [Text] / V. N. Petushkov, B. G. Gibson, J. . Lee // Biochemistry. - 1996. - Vol. 35, Is. 25. - P8413-8418, DOI 10.1021/bi952691v. - Cited References: 24 . - ISSN 0006-2960

РУБ Biochemistry & Molecular Biology
Рубрики:
LUMAZINE PROTEIN
   LUMINOUS BACTERIUM
   STRAIN Y-1
   BIOLUMINESCENCE
   EMISSION
   PURIFICATION
   TRANSIENT
   LIGHT
Аннотация: Time-resolved fluorescence was used to directly measure the energy transfer rate constant in the protein-protein complex involved in the yellow bioluminescence of Vibrio fischeri, strain Y1. In this reaction the putative donor is the fluorescent transient intermediate, luciferase hydroxyflavin, which exhibits a major fluorescence lifetime of the bound flavin of 10 ns. On addition of the acceptor, the V. fischeri yellow fluorescence protein containing either FMN or riboflavin as ligand, a rapid decay time, 0.25 ns, becomes predominant. The same results are observed using rec-luciferase from Photobacterium leiognathi to produce the donor. Because of favorable spectral separation in this system, this rapid decay rate of 4 ns(-1), can be directly equated to the energy transfer rate. This rate is ten times higher than the rate previously observed in the Photobacterium luciferase hydroxyflavin-lumazine protein, donor-acceptor system, derived from emission anisotropy measurements. This ten-times ratio is close to the ratio of spectral overlaps of the donor fluorescence with the acceptor absorption, between these two systems, so it is concluded that the topology of the protein complexes in both cases, must be very similar. Energy transfer is also monitored by the loss of steady-state fluorescence intensity at 460 nm of the donor, on addition of the acceptor protein. A fluorescence titration indicates that luciferase hydroxyflavin and the yellow protein complex with a 1:1 stoichiometry with a K-d of 0.7 mu M (0 degrees C). These parameters account for the bioluminescence spectral shifting effects observed in these reactions.

Держатели документа:
UNIV GEORGIA,DEPT BIOCHEM & MOLEC BIOL,ATHENS,GA 30602
RUSSIAN ACAD SCI,INST BIOPHYS,SIBERIAN BRANCH,KRASNOYARSK 660036,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J...

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17.

Characterization of hydromedusan Ca2+-regulated photoproteins as a tool for measurement of Ca2+concentration / N. P. Malikova [et al.] // . - 2014, DOI 10.1007/s00216-014-7986-2 . - ISSN 1618-2642
Кл.слова (ненормированные):
Aequorin -- Calcium -- Clytin -- Coelenterazine -- Mitrocomin -- Obelin
Аннотация: Calcium ion is a ubiquitous intracellular messenger, performing this function in many eukaryotic cells. To understand calcium regulation mechanisms and how disturbances of these mechanisms are associated with disease states, it is necessary to measure calcium inside cells. Ca2+-regulated photoproteins have been successfully used for this purpose for many years. Here we report the results of comparative studies on the properties of recombinant aequorin from Aequorea victoria, recombinant obelins from Obelia geniculata and Obelia longissima, recombinant mitrocomin from Mitrocoma cellularia, and recombinant clytin from Clytia gregaria as intracellular calcium indicators in a set of identical in vitro and in vivo experiments. Although photoproteins reveal a high degree of identity of amino acid sequences and spatial structures, and, apparently, have a common mechanism for the bioluminescence reaction, they were found to differ in the Ca2+ concentration detection limit, the sensitivity of bioluminescence to Mg2+, and the rates of the rise of the luminescence signal with a sudden change of Ca2+ concentration. In addition, the bioluminescence activities of Chinese hamster ovary cells expressing wild-type photoproteins also differed. The light signals of cells expressing mitrocomin, for example, slightly exceeded the background, suggesting that mitrocomin may be hardly used to detect intracellular Ca2+ without modifications improving its properties. On the basis of experiments on the activation of endogenous P2Y2 receptor in Chinese hamster ovary cells by ATP, we suggest that wild-type aequorin and obelin from O. longissima are more suitable for calcium detection in cytoplasm, whereas clytin and obelin from O. geniculata can be used for calcium measurement in cell compartments with high Ca2+ concentration. [Figure not available: see fulltext.] © 2014 Springer-Verlag Berlin Heidelberg.

Scopus
Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation
Laboratory of Bioluminescent Biotechnologies, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Malikova, N.P.; Burakova, L.P.; Markova, S.V.; Vysotski, E.S.

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18.

Characterization of hydromedusan Ca2+-regulated photoproteins as a tool for measurement of Ca(2+)concentration [Text] / N. P. Malikova [et al.] // Anal. Bioanal. Chem. - 2014. - Vol. 406, Is. 23. - P5715-5726, DOI 10.1007/s00216-014-7986-2. - Cited References: 67. - This work was supported by RFBR grant 12-04-00131, by the programs of the Government of the Russian Federation "Measures to Attract Leading Scientists to Russian Educational Institutions" (grant 11.G34.31.0058) and "Molecular and Cellular Biology" of the Russian Academy of Sciences, and the grant from the President of the Russian Federation "Leading Science School" (3951.2012.4). . - ISSN 1618-2642. - ISSN 1618-2650

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
LIGHT-SENSITIVE PHOTOPROTEIN
   CTENOPHORE BEROE ABYSSICOLA
   GREEN-FLUORESCENT PROTEIN
   INTRACELLULAR CALCIUM
   SEQUENCE-ANALYSIS
   CA-2+-ACTIVATED PHOTOPROTEIN
   CA2+-BINDING PHOTOPROTEIN
   SEMISYNTHETIC AEQUORINS
   LUMINESCENT PROTEIN
   RECOMBINANT OBELIN
Кл.слова (ненормированные):
Calcium -- Coelenterazine -- Aequorin -- Obelin -- Clytin -- Mitrocomin
Аннотация: Calcium ion is a ubiquitous intracellular messenger, performing this function in many eukaryotic cells. To understand calcium regulation mechanisms and how disturbances of these mechanisms are associated with disease states, it is necessary to measure calcium inside cells. Ca2+-regulated photoproteins have been successfully used for this purpose for many years. Here we report the results of comparative studies on the properties of recombinant aequorin from Aequorea victoria, recombinant obelins from Obelia geniculata and Obelia longissima, recombinant mitrocomin from Mitrocoma cellularia, and recombinant clytin from Clytia gregaria as intracellular calcium indicators in a set of identical in vitro and in vivo experiments. Although photoproteins reveal a high degree of identity of amino acid sequences and spatial structures, and, apparently, have a common mechanism for the bioluminescence reaction, they were found to differ in the Ca2+ concentration detection limit, the sensitivity of bioluminescence to Mg2+, and the rates of the rise of the luminescence signal with a sudden change of Ca2+ concentration. In addition, the bioluminescence activities of Chinese hamster ovary cells expressing wild-type photoproteins also differed. The light signals of cells expressing mitrocomin, for example, slightly exceeded the background, suggesting that mitrocomin may be hardly used to detect intracellular Ca2+ without modifications improving its properties. On the basis of experiments on the activation of endogenous P2Y(2) receptor in Chinese hamster ovary cells by ATP, we suggest that wild-type aequorin and obelin from O. longissima are more suitable for calcium detection in cytoplasm, whereas clytin and obelin from O. geniculata can be used for calcium measurement in cell compartments with high Ca2+ concentration.

WOS
Держатели документа:
[Malikova, Natalia P.
Burakova, Ludmila P.
Markova, Svetlana V.
Vysotski, Eugene S.] Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia
[Malikova, Natalia P.
Burakova, Ludmila P.
Markova, Svetlana V.
Vysotski, Eugene S.] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Malikova, N.P.; Burakova, L.P.; Markova, S.V.; Vysotski, E.S.; RFBR [12-04-00131]; Government of the Russian Federation [11.G34.31.0058]; Russian Academy of Sciences; Russian Federation "Leading Science School" [3951.2012.4]

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19.

UNDERWAY MEASUREMENT OF BIOMINESCENT FIELD DURING BIOLOGICAL EXPEDITION [Текст] / I. I. GITELSON, R. N. UTYUSHEV, A. V. SHELEGOV // Okeanologiya. - 1991. - Vol. 31, Is. 4. - P. 631-637. - Cited References: 5 . - ISSN 0030-1574

РУБ Oceanography

Аннотация: Data on surface bioluminescence intensity measurements during the 15-th cruise of R/V Vityaz, January to March 1988, in the Indian Ocean, the Mediterranean and Black Seas are presented. High luminescence intensity is found in the Arabian and Black Seas and the Dardanelles. Recommendations on underway measurements of biological and hydrophysical parameters are given.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GITELSON, I.I.; UTYUSHEV, R.N.; SHELEGOV, A.V.

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20.

Evaluation of the effect of light intensity on the measurement of the photosynthetic rate in plankton microalgae by the chlorophyll fluorescence method [Text] / N. A. Gaevskii [et al.] // Russ. J. Plant Physiol. - 2000. - Vol. 47, Is. 6. - P. 820-825, DOI 10.1023/A:1026671531500. - Cited References: 21 . - ISSN 1021-4437

РУБ Plant Sciences
Рубрики:
QUANTUM YIELDS
   PHYTOPLANKTON
   CHLOROPLASTS
   ALGAE
Кл.слова (ненормированные):
fluorescence -- chlorophyll -- microalgae -- photosynthetic rate -- photosynthetic activity
Аннотация: The use of relative variable fluorescence (RVF) of chlorophyll, as measured in the presence of Diuron, an inhibitor of electron transfer, for the estimation of the photosynthetic activity of plankton microalgae was analyzed under a wide range of light intensities in the PAR region. Oxygen evolution rates (estimated by the method of light and dark bottles and the amperometric method), RVF, and chlorophyll a concentration were measured in parallel in natural algal cenoses and microecosystems. When the previously used regression equation, in the form A = b(DeltaF/F-d)CchlI, where A is O-2 evolution rate (g/(m(3) h), DeltaF/F-d is RVF (relative units), C-chl is chlorophyll a concentration (mg/m(3)), and I is light intensity (W/m(2)), was verified in the PAR region, we observed a nonlinear dependence of the correction coefficient b on I, which can be described by the formula b = 6.227 x 10(3)rootI. This result agrees with the hypothesis that chlorophyll a fluorescence quenching comprises photochemical (qQ) and energy (qE) components. On the basis of the energy model, we determined the upper limit b(max) = 0.003 for light intensity range I < 4.4 W/m(2) and the lower limit b(min) = 0.0003 for I = 400 W/m(2).

WOS
Держатели документа:
Krasnoyarsk State Univ, Dept Biol, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gaevskii, N.A.; Kolmakov, V.I.; Popel'nitskii, V.A.; Gold, V.M.; Dubovskaya, O.P.

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