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Intracellular phosphorus pool of the cyanobacterium spirulina platensis / A. Ya. Bolsunovsk, S. V. Kosinenko // Mikrobiologiya. - 2000. - Vol. 69, Is. 1. - С. 135-137 . - ISSN 0026-3656
Кл.слова (ненормированные):
phosphorus -- article -- biomass -- Cyanobacterium -- growth, development and aging -- metabolism -- Biomass -- Cyanobacteria -- Phosphorus
Аннотация: An intracellular phosphorus pool in a monoculture of the cyanobacterium Spirulina platensis was assessed using radioactive and nonradioactive phosphorus. The derived dependence of specific growth rate on the intracellular content of mineral phosphorus can be presented in the form of the Droop equation. It was found that the stage of replenishment of the intracellular phosphorus pool may affect the phosphorus turnover estimation in aquatic environments from the results of short-term measurements of phosphorus uptake.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsk, A.Ya.; Kosinenko, S.V.

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Detoxification of AM-241 solutions by humic substances: Bioluminescent monitoring / T. Rozhko [et al.] // Analytical and Bioanalytical Chemistry. - 2011. - Vol. 400, Is. 2. - P329-334, DOI 10.1007/s00216-010-4442-9 . - ISSN 1618-2642
Кл.слова (ненормированные):
Detoxification -- Humic substances -- Ionizing radiation -- Luminous bacteria -- Bacterial cells -- Bottom sediments -- Humic substances -- Luminescent intensity -- Luminous bacteria -- Natural transformations -- Organic substances -- Photobacterium phosphoreum -- Physiological activity -- Protecting agent -- Water solutions -- Anoxic sediments -- Bacteriology -- Bioluminescence -- Detoxification -- Ionizing radiation -- Luminance -- Radiation shielding -- Radioactivity -- Bacteria -- americium -- radioisotope -- article -- bioremediation -- chemistry -- environmental monitoring -- evaluation -- humic substance -- instrumentation -- luminescence -- metabolism -- methodology -- Photobacterium -- water pollutant -- Americium -- Biodegradation, Environmental -- Environmental Monitoring -- Humic Substances -- Luminescence -- Photobacterium -- Radioisotopes -- Water Pollutants, Chemical -- Bacteria (microorganisms) -- Photobacterium phosphoreum
Аннотация: The study addresses the effect of humic substances on marine luminous bacteria Photobacterium phosphoreum exposed to Am-241 (3,000 Bq L -1, water solution). Luminescent intensity of the bacteria was applied as a marker of their physiological activity. Humic substances have been found to reduce the effect of Am-241 on luminescence, decrease damage to cells, and change distribution of Am-241 between bacterial cells and intercellular media. It was shown that water-soluble humic substances, being products of natural transformation of organic substances in soil and bottom sediments, can serve as protecting agents for water microorganisms exposed to alpha radionuclides. В© 2010 Springer-Verlag.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk 660036, Russian Federation
Irkutsk State University, Irkutsk 664003, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rozhko, T.; Bondareva, L.; Mogilnaya, O.; Vydryakova, G.; Bolsunovsky, A.; Stom, D.; Kudryasheva, N.

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Microdistribution of 241Am in structures of submerged macrophyte Elodea canadensis growing in the Yenisei River / L. Bondareva [et al.] // Journal of Environmental Radioactivity. - 2010. - Vol. 101, Is. 1. - P16-21, DOI 10.1016/j.jenvrad.2009.08.003 . - ISSN 0265-931X
Кл.слова (ненормированные):
Alpha-track analysis -- Americium -- Elodea canadensis -- Liquid-scintillation spectrometry -- Microdistribution -- Alpha-track analysis -- Elodea canadensis -- Microdistribution -- Scintillation spectrometry -- Track analysis -- Liquids -- Luminescence -- Radioisotopes -- Scintillation -- Spectrometry -- Spectroscopy -- Americium -- americium 241 -- americium -- americium -- bioaccumulation -- experimental study -- leaf -- macrophyte -- mass spectrometry -- morphology -- radionuclide -- spatial distribution -- stem -- submerged vegetation -- tracking -- aquatic flora -- article -- bioaccumulation -- concentration (parameters) -- elodea canadensis -- environmental radioactivity -- isotope analysis -- isotope tracing -- leaf lamina -- leaf surface -- macrophyte -- nonhuman -- plant cell -- plant morphology -- plant stem -- radioisotope distribution -- river -- chemistry -- Hydrocharitaceae -- metabolism -- plant -- plant leaf -- radiation monitoring -- river -- water pollutant -- Eurasia -- Russian Federation -- Yenisei River -- Elodea canadensis -- Americium -- Hydrocharitaceae -- Plant Leaves -- Plant Shoots -- Plant Stems -- Radiation Monitoring -- Rivers -- Water Pollutants, Radioactive
Аннотация: A submerged macrophyte of the Yenisei River, Elodea canadensis, was used to study the microdistribution of the artificial radionuclide 241Am among different components of the plant. The total amount of 241Am added to the experimental system was 1850 В± 31 Bq/L. The total amount of 241Am accumulated by the plants was 182 Bq per sample, or 758,333 В± 385 Bq/kg dry mass. It has been found that the major portion of 241Am accumulated by E. canadensis, up to 85%, was bound to solid components of the cells. It is observed that the microdistribution of 241Am within different components of the submerged plant E. canadensis was not uniform. 241Am distribution vary depending on the age of the leaf blades, the state of the cells and morphological features of the plant stem. В© 2009 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.; Vlasova, I.; Mogilnaya, O.; Bolsunovsky, A.; Kalmykov, S.

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Microbiological and isotopic-geochemical investigations of meromictic lakes in Khakasia in winter / A. S. Savvichev [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 4. - С. 552-561 . - ISSN 0026-3656
Кл.слова (ненормированные):
Meromictic water bodies -- Microbial production and oxidation of methane -- Photosynthesis -- Stable isotopes of carbon (? 13C) and sulfur (? 34S) -- Sulfate reduction -- Bacteria (microorganisms) -- Chlorobi -- Chromatiaceae -- Lamprocystis purpurea -- Pelodictyon luteolum -- Photobacteria -- carbon -- fresh water -- methane -- sulfate -- sulfur -- article -- bacterial phenomena and functions -- bacterium -- comparative study -- isolation and purification -- metabolism -- microbiology -- oxidation reduction reaction -- photosynthesis -- Russian Federation -- season -- species difference -- Bacteria -- Bacterial Physiology -- Carbon Isotopes -- Fresh Water -- Methane -- Oxidation-Reduction -- Photosynthesis -- Seasons -- Siberia -- Species Specificity -- Sulfates -- Sulfur Isotopes -- Water Microbiology
Аннотация: Microbiological and isotopic-geochemical investigations of the brackish meromictic lakes Shira and Shunet were performed in the steppe region of Khakasia in winter. Measurements made with a submersed sensor demonstrated that one-meter ice transmits light in a quantity sufficient for oxygenic and anoxygenic photosynthesis. As in the summer season, in the community of phototrophic bacteria found in Lake Shira, the purple sulfur bacteria Amoebobacter purpureus dominated, whereas, in Lake Shunet, the green sulfur bacteria Pelodictyon luteolum were predominant. Photosynthetic production, measured using the radioisotopic method, was several times lower than that in summer. The rates of sulfate reduction and production and oxidation of methane in the water column and bottom sediments were also lower than those recorded in summer. The process of anaerobic methane oxidation in the sediments was an exception, being more intense in winter than in summer. The data from radioisotopic measurements of the rates of microbial processes correlate well with the results of determination of the isotopic composition of organic and mineral carbon (? 13C) and hydrogen sulfide and sulfate (? 34S) and suggest considerable seasonal variations in the activity of the microbial community in the water bodies investigated.

Scopus
Держатели документа:
Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya, Moscow, 117811, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk 36, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Savvichev, A.S.; Rusanov, I.I.; Rogozin, D.Yu.; Zakharova, E.E.; Lunina, O.N.; Bryantseva, I.A.; Yusupov, S.K.; Pimenov, N.V.; Degermendzhi, A.G.; Ivanov, M.V.

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Structure of hydrocarbons synthesized by the alga Botryococcus isolated from Lake Shira. / N. O. Zhila [et al.] // Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. - 2001. - Vol. 378. - P265-269 . - ISSN 0012-4966
Кл.слова (ненормированные):
fatty acid -- hydrocarbon -- sea water -- article -- chemical structure -- chemistry -- classification -- green alga -- isolation and purification -- metabolism -- microbiology -- Russian Federation -- species difference -- Algae, Green -- Fatty Acids -- Hydrocarbons -- Molecular Structure -- Seawater -- Siberia -- Species Specificity

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhila, N.O.; Kalacheva, G.S.; Volova, T.G.; Degermendzhi, A.G.

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Carotenoids of phototrophic organisms in bottom sediments of meromictic Lake Shira (Siberia, Russia) as an indicator of past stratification / D. Y. Rogozin [et al.] // Doklady Biological Sciences. - 2011. - Vol. 439, Is. 1. - P228-231, DOI 10.1134/S0012496611040077 . - ISSN 0012-4966
Кл.слова (ненормированные):
carotenoid -- chlorophyll -- water -- animal -- article -- chemistry -- ecosystem -- lake -- metabolism -- microbiology -- photosynthesis -- phototrophy -- pigmentation -- Russian Federation -- sediment -- Animals -- Carotenoids -- Chlorophyll -- Ecosystem -- Geologic Sediments -- Lakes -- Photosynthesis -- Phototrophic Processes -- Pigmentation -- Russia -- Siberia -- Water -- Water Microbiology

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 5050, Krasnoyarsk 660036, Russian Federation
Institute of Geology and Mineralogy, Siberian Branch, Russian Academy of Sciences, pr. Akademika Koptyuga 3, Novosibirsk 630090, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rogozin, D.Y.; Zykov, V.V.; Kalugin, I.A.; Daryin, A.V.; Degermendzhy, A.G.

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241Am distribution in the biomass of freshwater macrophytes / T. A. Zotina [et al.] // Doklady Biological Sciences. - 2008. - Vol. 421, Is. 1. - P254-256, DOI 10.1134/S0012496608040108 . - ISSN 0012-4966
Кл.слова (ненормированные):
americium -- carbohydrate -- cellulose -- lipid -- nitrogen -- polysaccharide -- vegetable protein -- article -- biomass -- Bryopsida -- cell membrane -- cell wall -- chemistry -- cytoplasm -- food chain -- growth, development and aging -- Hydrocharitaceae -- metabolism -- Americium -- Biomass -- Bryopsida -- Carbohydrates -- Cell Membrane -- Cell Wall -- Cellulose -- Cytoplasm -- Food Chain -- Hydrocharitaceae -- Lipids -- Nitrogen -- Plant Proteins -- Polysaccharides

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.A.; Kalachova, G.S.; Bolsunovsky, A.Ya.; Degermendzhy, A.G.

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The prospects of the use of resorbable polyesters for designing safe pesticides / T. G. Volova [et al.] // Doklady Biological Sciences. - 2008. - Vol. 419, Is. 1. - P100-103, DOI 10.1134/S0012496608020099 . - ISSN 0012-4966
Кл.слова (ненормированные):
pesticide -- polyester -- article -- chemistry -- drug design -- metabolism -- microbiology -- Drug Design -- Pesticides -- Polyesters -- Soil Microbiology

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Kransnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinova, O.N.; Kalacheva, G.S.; Grodnitskaya, I.D.

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Luminous bacteria as potential producers of resorbed polyhydroxyalkanoate polyesters / A. N. Boyandin [et al.] // Doklady Biochemistry and Biophysics. - 2007. - Vol. 416, Is. 1-6. - P248-251, DOI 10.1134/S1607672907050067 . - ISSN 1607-6729
Кл.слова (ненормированные):
photoprotein -- polyester -- polyhydroxyalkanoic acid -- article -- bacterium -- chemistry -- chemoluminescence -- classification -- genetic procedures -- genetics -- metabolism -- Photobacterium -- phylogeny -- time -- Bacteria -- Biosensing Techniques -- Chemiluminescent Measurements -- Luminescent Proteins -- Photobacterium -- Phylogeny -- Polyesters -- Polyhydroxyalkanoates -- Time Factors -- Bacteria (microorganisms)

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Kalacheva, G.S.; Rodicheva, E.K.; Volova, T.G.

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10.

Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. / T. G. Volova [et al.] // Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
beta hydroxyvaleric acid -- beta-hydroxyvaleric acid -- carbon dioxide -- ester -- fatty acid -- fructose -- glucose -- hexanoic acid derivative -- hydrogen -- hydroxyacid -- oxygen -- polymer -- ribosome RNA -- valeric acid derivative -- article -- biotechnology -- chemistry -- metabolism -- methodology -- Wautersia eutropha -- Biotechnology -- Carbon Dioxide -- Cupriavidus necator -- Esters -- Fatty Acids -- Fructose -- Glucose -- Hexanoic Acids -- Hydrogen -- Hydroxy Acids -- Oxygen -- Pentanoic Acids -- Polymers -- RNA, Ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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11.

The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69 . - ISSN 0026-3656
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Cupriavidus necator -- acetoacetyl coenzyme a -- acetyl coenzyme A acyltransferase -- acyl coenzyme A -- acyltransferase -- butyric acid derivative -- carbon dioxide -- fructose -- hydrogen -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3-hydroxyalkanoic acid) synthase -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- polyester -- polyhydroxyalkanoate synthase -- valeric acid derivative -- article -- chemistry -- crystallization -- culture medium -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyl Coenzyme A -- Acyltransferases -- Butyrates -- Carbon Dioxide -- Crystallization -- Culture Media -- Cupriavidus necator -- Fructose -- Hydrogen -- Polyesters -- Valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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12.

Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562 / T. G. Volova [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 6. - С. 788-794 . - ISSN 0026-3656
Кл.слова (ненормированные):
Glucose-utilizing strain -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates -- Synthesis -- Bacteria (microorganisms) -- Cupriavidus necator -- bacterial RNA -- glucose -- hydrogen -- hydroxybutyric acid -- polymer -- RNA 16S -- article -- culture medium -- genetics -- growth, development and aging -- metabolism -- mutation -- oxidation reduction reaction -- physiology -- Wautersia eutropha -- Culture Media -- Cupriavidus necator -- Glucose -- Hydrogen -- Hydroxybutyrates -- Mutation -- Oxidation-Reduction -- Polymers -- RNA, Bacterial -- RNA, Ribosomal, 16S
Аннотация: The physiological, biochemical, genetic, and cultural characteristics of the glucose-utilizing mutant strain Ralstonia eutropha B8562 were investigated in comparison with the parent strain R. eutropha B5786. The morphological, cultural, and biochemical characteristics of strain R. eutropha B8562 were similar to those of strain R. eutropha B5786. Genetic analysis revealed differences between the 16S rRNA gene sequences of these strains. The growth characteristics of the mutant using glucose as the sole carbon and energy source were comparable with those of the parent strain grown on fructose. Strain B8562 was characterized by high yields of polyhydroxyalkanoate (PHA) from different carbon sources (CO 2, fructose, and glucose). In batch culture with glucose under nitrogen limitation, PHA accumulation reached 90% of dry weight. In PHA, ?-hydroxybutyrate was predominant (over 99 mol %); ?-hydroxyvalerate (0.25-0.72 mol %) and ?-hydroxyhexanoate (0.008-1.5 mol %) were present as minor components. The strain has prospects as a PHA producer on glucose-containing media.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kozhevnikov, I.V.; Dolgopolova, Yu.B.; Trusova, M.Yu.; Kalacheva, G.S.; Aref'eva, Yu.V.

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13.

Study of Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing / T. G. Volova, N. A. Voinov // Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 3. - С. 296-300 . - ISSN 0555-1099
Кл.слова (ненормированные):
carbon monoxide -- coal -- polyester -- antibiotic resistance -- article -- culture medium -- enzyme specificity -- gas -- growth, development and aging -- metabolism -- microbiology -- Wautersia eutropha -- Carbon Monoxide -- Coal -- Culture Media -- Cupriavidus necator -- Drug Resistance, Microbial -- Gases -- Polyesters -- Substrate Specificity
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange have been studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of the substrate utilization (up to 90%).

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of the Russian Academy of Sciences, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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14.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786 / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 2. - P170-177, DOI 10.1023/B:ABIM.0000018921.04863.d5 . - ISSN 0003-6838
Кл.слова (ненормированные):
acetyl coenzyme A acyltransferase -- bacterial enzyme -- carbon -- carbon dioxide -- fructose -- hydrogen -- hydroxybutyrate dehydrogenase -- oxidoreductase -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- synthetase -- article -- bacterial metabolism -- carbon source -- catalysis -- controlled study -- degradation -- depolymerization -- enzyme activity -- enzyme analysis -- molecular dynamics -- nonhuman -- protein function -- Ralstonia eutropha -- recording -- statistical significance -- synthesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (?-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of ?-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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15.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha / T. G. Volova [и др.] // Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 2. - С. 201-209 . - ISSN 0555-1099
Кл.слова (ненормированные):
acetoacetyl coenzyme a reductase -- acetoacetyl-CoA reductase -- acetyl coenzyme A acyltransferase -- acyltransferase -- alcohol dehydrogenase -- carboxylesterase -- hydroxybutyrate dehydrogenase -- hydroxybutyric acid -- poly(3 hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) synthase -- polyhydroxyalkanoate synthase -- polymer -- article -- chemistry -- comparative study -- culture medium -- enzymology -- growth, development and aging -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyltransferases -- Alcohol Oxidoreductases -- Carboxylic Ester Hydrolases -- Culture Media -- Cupriavidus necator -- Hydroxybutyrate Dehydrogenase -- Hydroxybutyrates -- Polymers
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of the key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, D-hydroxybutyrate dehydrogenase, and PHA depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase were recorded at the stage of acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only at stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not cause any marked changes in the time course of enzyme activity.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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16.

Kinetic parameters of a culture of the hydrogen-oxidizing Ralstonia eutropha, grown under the regimen of biosynthesis of polyhydroxybutyrate / T. G. Volova, N. A. Voinov // Prikladnaia biokhimiia i mikrobiologiia. - 2003. - Vol. 39, Is. 2. - С. 189-193 . - ISSN 0555-1099
Кл.слова (ненормированные):
hydrogen -- hydroxybutyric acid -- polyester -- article -- culture medium -- enzyme specificity -- growth, development and aging -- kinetics -- metabolism -- oxidation reduction reaction -- temperature -- Wautersia eutropha -- Culture Media -- Cupriavidus necator -- Hydrogen -- Hydroxybutyrates -- Kinetics -- Oxidation-Reduction -- Polyesters -- Substrate Specificity -- Temperature
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha, grown on a gas substrate under the conditions favoring autotrophic biosynthesis of polyhydroxybutyrate, were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of culture medium, and coefficients of heat emission and mass transfer.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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17.

The effect of temperature on the lipid composition of Botryococcus / G. S. Kalacheva [и др.] // Mikrobiologiya. - 2002. - Vol. 71, Is. 3. - С. 336-344 . - ISSN 0026-3656
Кл.слова (ненормированные):
fatty acid -- lipid -- triacylglycerol -- article -- biosynthesis -- chemistry -- comparative study -- green alga -- growth, development and aging -- metabolism -- temperature -- Algae, Green -- Fatty Acids -- Lipids -- Temperature -- Triglycerides
Аннотация: The lipid composition of the green alga Botryococcus was studied at three different cultivation temperatures: suboptimal, optimal, and supraoptimal. Cultivation at the supraoptimal temperature was found to considerably inhibit the synthesis of nearly all intracellular lipids, except for triacylglycerides, and to influence their fatty acid composition. In particular, the content of trienoic fatty acids was significantly lower at the supraoptimal than at the optimal cultivation temperature. At the same time, the fatty acid composition of the extracellular lipids of the alga virtually did not depend on cultivation temperature.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zhila, N.O.; Volova, T.G.; Gladyshev, M.I.

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18.

On the involvement of macrophages and phosphomonoesterases in the tissue response to implantation of polyhydroxyalkanoates. / E. I. Shishatskaya, T. G. Volova, I. I. Gitelson // Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - P116-119 . - ISSN 0012-4966
Кл.слова (ненормированные):
alkane -- phosphatase -- animal -- article -- comparative study -- foreign body reaction -- macrophage -- metabolism -- physiology -- rat -- Wistar rat -- Alkanes -- Animals -- Foreign-Body Reaction -- Macrophages -- Phosphoric Monoester Hydrolases -- Rats -- Rats, Wistar

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gitelson, I.I.

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19.

Tissue response to biodegradable suture threads made of polyoxyalkanoates / E. I. Shishatskaya [и др.] // Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 23-29 . - ISSN 0025-8075
Кл.слова (ненормированные):
Biocompatibility -- Biodegradation -- Implants (surgical) -- Materials testing -- Organic polymers -- Polyoxyalkanoates -- Suture threads -- Biomaterials -- biomaterial -- polymer -- animal -- article -- bioremediation -- female -- metabolism -- rat -- suture -- Wistar rat -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Female -- Polymers -- Rats -- Rats, Wistar -- Sutures
Аннотация: The paper presents data on the materials and methods of obtaining suture threads based on of the polymer batty acid derivatives (polyoxyalkanoates). Results of the study of tissue response to biodegradable polyoxyalkanoate suture threads in the postoperative period are analyzed. These suture threads have been shown to have the required strength and meet a number of other requirements for currently available materials.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Efremova, S.N.; Puzyr, S.P.; Mogil'naya, O.A.

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20.

Study of the biological properties of polyoxyalkanoates in the in vivo chronic experiment / E. I. Shishatskaya, T. G. Volova, T. G. Popova // Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 29-33 . - ISSN 0025-8075
Кл.слова (ненормированные):
biomaterial -- polymer -- animal -- article -- bioremediation -- comparative study -- female -- metabolism -- rat -- suture -- Wistar rat -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Female -- Polymers -- Rats -- Rats, Wistar -- Sutures -- Biocompatibility -- Immunology -- Implants (surgical) -- Materials testing -- Organic polymers -- Polyoxyalkanoates -- Rat models -- Suture threads -- Biomaterials
Аннотация: Chronic experiments on mature Wistar rats studied the biological properties of suture threads from two types of polyoxyalkanoates (POA), such as polyoxybutyrate (POB) and the copolymer of POB and polyoxyvalerate (POV) (POB/POV) as compared to the conventional surgical material (silk and catgut). The physiological and biochemical parameters that reflect the status of the animals, including their behavior, height and development, the weight of the body and viscera, the morphology of peripheral blood, the biochemical parameters of blood, the activity of some enzymes, as well as histological findings of the viscera, and lymphoid tissue responses were assessed. POA was found to produce no negative effect on the physiological, biochemical, and functional characteristics of the animals irrespective of the duration of contact with the internal medium of the body and of the chemical composition of a material.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Popova, T.G.

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