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Seasonal features of consumption of lysine by uncultivated bacterial plankton of Eutrophic water reservoir / M. Y. Trusova, O. V. Kolmakova, M. I. Gladyshev // Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - P391-398, DOI 10.1134/S1995425512040154 . - ISSN 1995-4255
Кл.слова (ненормированные):
16S ribosomal RNA -- biogeochemical function -- denaturing gradient gel electrophoresis -- eutrophic water reservoir -- lysine -- microecosystem (MES) -- uncultivated bacterial plankton -- amino acid -- bacterioplankton -- bacterium -- biogeochemistry -- electrokinesis -- eutrophication -- laboratory method -- microbial community -- polymerase chain reaction -- reservoir -- seasonal variation -- specialization -- Bacteria (microorganisms)
Аннотация: Dynamics of bacterial plankton community of eutrophic water reservoir in laboratory microecosystems with amino acid lysine was studied using PCR-DGGE technique. The addition of lysine to the microecosystems resulted in changes in the composition of the bacterial plankton in summer; in particular, a number of Lys1 and Lys2 species (genotypes) that consume this amino acid grew fast in the bacterial community. The plank tonic bacterial communities did not respond to the addition of lysine in spring and late summer. The obtained data confirm the hypothesis for the narrow specialization of bacterial plankton species to the consumption of individual organic substances. В© 2012 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok 50-50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnii prosp. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Y.; Kolmakova, O.V.; Gladyshev, M.I.

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Population dynamics of transgenic microorganisms in the different microecosystem conditions / L. Yu. Popova [et al.] // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1571-1579, DOI 10.1016/S0273-1177(01)00248-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Ecosystems -- Escherichia coli -- Genes -- Interferons -- Luminescence -- Vaccines -- Microcosms -- Microorganisms -- fresh water -- microorganism -- adaptation -- article -- Bacillus subtilis -- comparative study -- ecosystem -- Escherichia coli -- gene expression -- genetics -- growth, development and aging -- hazardous waste -- microbiology -- Micrococcus -- osmotic pressure -- plasmid -- population dynamics -- risk assessment -- transgenic organism -- Adaptation, Physiological -- Bacillus subtilis -- Containment of Biohazards -- Ecosystem -- Escherichia coli -- Fresh Water -- Gene Expression -- Micrococcus -- Organisms, Genetically Modified -- Osmotic Pressure -- Plasmids -- Population Dynamics -- Risk Assessment -- Water Microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Maksimova, E.E.; Boyandin, A.N.; Pechurkin, N.S.

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A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2 [Text] / L. Y. Popova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 6. - P. 637-641. - Cited References: 13 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
Кл.слова (ненормированные):
microecosystem -- Bacillus subtilis -- recombinant plasmid -- interferon -- adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.

WOS
Держатели документа:
Russian Acad Sci, Akademgorodok, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
State Sci Ctr VB Vektor, Sci Res Design & Technol Inst Biol Act Subst, Berdsk 633190, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Y.; Kargatova, T.V.; Maksimova, E.E.; Belyavskaya, V.A.

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Population dynamics of transgenic microorganisms in the different microecosystem conditions [Text] / L. Y. Popova [et al.] ; ed. , AN Boyand // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P. 1571-1579, DOI 10.1016/S0273-1177(01)00248-4. - Cited References: 8 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
GENETICALLY-ENGINEERED MICROORGANISMS
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap(r)Lux(+)) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km(r)lnf(+)), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Y.; Lobova, T.I.; Krylova, T.Y.; Kargatova, T.V.; Maksimova, E.E.; Boyandin, A.N.; Pechurkin, N.S.; Boyand, , AN \ed.\

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