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1.


   
    The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties / A. Y. Gorokhovatsky, T. V. Chepurnykh, A. S. Shcheglov [et al.] // Doklad. Biochem. Biophys. - 2021. - Vol. 496, Is. 1. - P52-55, DOI 10.1134/S1607672921010051 . - ISSN 1607-6729
Кл.слова (ненормированные):
bioluminescence -- heterologous expression -- luciferase -- Neonothopanus nambi -- nnLuz -- Pichia pastoris
Аннотация: Abstract: A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase. © 2021, The Author(s),.

Scopus
Держатели документа:
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Gorokhovatsky, A. Y.; Chepurnykh, T. V.; Shcheglov, A. S.; Mokrushina, Y. A.; Baranova, M. N.; Goncharuk, S. A.; Purtov, K. V.; Petushkov, V. N.; Rodionova, N. S.; Yampolsky, I. V.

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2.


   
    The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties / A. Y. Gorokhovatsky, T. V. Chepurnykh, A. S. Shcheglov [et al.] // Dokl. Biochem. Biophys. - 2021. - Vol. 496, Is. 1. - P52-55, DOI 10.1134/S1607672921010051. - Cited References:10. - The work was supported by the Russian Science Foundation (project no. 16-14-00052-P). The creation of the luciferase-producing yeast strain nnLuz was supported by the President's grant for state support of the leading scientific schools of the Russian Federation (NSh-2605.2020.4). . - ISSN 1607-6729. - ISSN 1608-3091
РУБ Biochemistry & Molecular Biology + Biophysics

Кл.слова (ненормированные):
bioluminescence -- luciferase -- nnLuz -- Neonothopanus nambi -- heterologous -- expression -- Pichia pastoris
Аннотация: A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase.

WOS
Держатели документа:
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia.
Russian Acad Sci, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr,Siberian Branch, Krasnoyarsk, Russia.

Доп.точки доступа:
Gorokhovatsky, A. Yu; Chepurnykh, T., V; Shcheglov, A. S.; Mokrushina, Yu A.; Baranova, M. N.; Goncharuk, S. A.; Purtov, K., V; Petushkov, V. N.; Rodionova, N. S.; Yampolsky, I., V; Russian Science FoundationRussian Science Foundation (RSF) [16-14-00052-P]; President's grant for state support of the leading scientific schools of the Russian Federation [NSh-2605.2020.4]

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