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A study of spectral-polarization characteristics of plant canopies using land-based remote sensing / A. F. Sid'ko [et al.] // Journal of Quantitative Spectroscopy and Radiative Transfer. - 2013. - Vol. 129. - P109-117, DOI 10.1016/j.jqsrt.2013.06.001 . - ISSN 0022-4073
Кл.слова (ненормированные):
Farm crops -- Forest stands -- Spectral brightness coefficients -- Spectral-polarization characteristics -- Forest stand -- Near-infrared spectral regions -- Plant reflectance -- Polarized components -- Reflectance spectrum -- Reflection properties -- Spectral brightness -- Spectral-polarization characteristics -- Forestry -- Luminance -- Physiological models -- Polarization -- Reflection -- Crops -- brightness temperature -- canopy reflectance -- crop plant -- nadir -- polarization -- remote sensing -- spectral analysis -- Farm Crops -- Forestry -- Forests -- Polarization -- Reflection -- Triticum aestivum -- Zea mays
Аннотация: The study addresses reflection and spectral-polarization characteristics of forest stands and farm crops obtained under field conditions. The study of the reflection properties of farm crops shows that during the summer plant growing season, the major factors influencing the plant canopy reflectance are morpho-physiological parameters, plant architectonics, solar elevation h0, and viewing angle. The crop reflectance minimum was recorded at viewing angles 25-30В° with respect to the nadir. Coniferous and broadleaf forest stands had similar reflectance spectra of polarized light. The polarized component was smaller for all coniferous stands than for broadleaf ones. For broad-leaved farm crops (wheat and corn), the polarized component of the spectral brightness coefficients had a greater influence on the plant reflectance in the red and near-infrared spectral regions, ?>720nm. В© 2013 Elsevier Ltd.

Scopus
Держатели документа:
Institute of Biophysics of the Siberian Branch of RAS, 50-50 Akademgorodok, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sid'ko, A.F.; Botvich, I.; Pisman, T.I.; Shevyrnogov, A.P.

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Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates / V. N. Petushkov, B. G. Gibson, J. Lee // Biochemistry. - 1995. - Vol. 34, Is. 10. - P3300-3309 . - ISSN 0006-2960
Кл.слова (ненормированные):
luciferase -- recombinant protein -- article -- ligand binding -- nonhuman -- priority journal -- protein isolation -- protein protein interaction -- protein stability -- vibrionaceae -- Bacterial Proteins -- Binding Sites -- Carrier Proteins -- Circular Dichroism -- Flavin Mononucleotide -- Fluorescence Polarization -- Genes, Bacterial -- Kinetics -- Ligands -- Luciferase -- Luminescence -- Molecular Sequence Data -- Photobacterium -- Recombinant Proteins -- Spectrophotometry -- Support, U.S. Gov't, P.H.S. -- Photobacterium leiognathi -- Vibrionaceae
Аннотация: Ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (EMBL X56534) of Photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and SDS-PAGE. Scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30В°C the KdS (?M) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound FMN, 30. All holoproteins are highly fluorescent and have absorption spectra distinct from each other and from the free ligands. The longest wavelength absorption maxima are, respectively (nm, 2В°C), 420,463, and 458. Ligand binding produces no change in the far-UV circular dichroism; all have mean residual ellipticity at 210 nm of -6500 deg cm2 dmol-1, the same as the native protein. However, in the bioluminescence reaction only the lumazine holoprotein shows a bioluminescence effect. Fluorescence emission anisotropy decay was used to establish that none of these holoproteins complexed with native luciferase and that the lumazine protein alone formed a 1:1 complex with the luciferase hydroxyflavin fluorescent transient and the luciferase peroxyflavin intermediates, revealed by a dominant channel of anisotropy loss, with rotational correlation time of 2.5 ns, and attributed to excitation transfer from the luciferase flavin donor to the acceptor, the lumazine ligand. The complex stability was sufficient to allow its isolation by FPLC gel filtration and verification by SDS-PAGE. These methods also confirmed the absence of interaction of the holoflavoproteins.

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Держатели документа:
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States
Institute of Biophysics, Academy of Sciences of Russia (Siberian Branch), 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J.

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Analysis of polarization characteristics of plant canopies using ground-based remote sensing measurements [Text] / A. F. Sid'ko [et al.] // J. Quant. Spectrosc. Radiat. Transf. - 2014. - Vol. 144. - P117-122, DOI 10.1016/j.jqsrt.2014.03.031. - Cited References: 26 . - ISSN 0022-4073. - ISSN 1879-1352

РУБ Spectroscopy
Рубрики:
LINEAR-POLARIZATION
   AGRICULTURAL CROPS
   WHEAT CANOPIES
   LIGHT
   REFLECTANCE
   VEGETATION
Кл.слова (ненормированные):
Spectral brightness coefficients -- Degree of polarization -- Polarized component of spectral brightness coefficients -- Farm crop -- Coniferous and broadleaf forests
Аннотация: The paper presents results and analysis of a study on polarized characteristics of the reflectance factor of different plant canopies under field conditions, using optical remote sensing techniques. Polarization characteristics were recorded from the elevated work platform at heights of 10-18 m in June and July. Measurements were performed using a double-beam spectrophotometer with a polarized light filter attachment, within the spectral range from 400 to 820 nm. The viewing zenith angle was below 20 degree. Birch (Betila pubescens), pine (Pinus sylvestris L.), wheat (Triticum acstivum) [L.] crops, corn (Zea mays L ssp. mays) crops, and various grass canopies were used in this study. The following polarization characteristics were studied: the reflectance factor of the canopy with the polarizer adjusted to transmit the maximum and minimum amounts of light (R-max and R-min), polarized component of the reflectance factor (R-q), and the degree of polarization (P). Wheat, corn, and grass canopies have higher R-max and R-min values than forest plants. The R-q and P values are higher for the birch than for the pine within the wavelength range between 430 and 740 nm. The study shows that polarization characteristics of plant canopies may be used as an effective means of decoding remote sensing data. (C) 2014 Elsevier Ltd. All rights reserved.

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Держатели документа:
[Sid'ko, A. F.
Botvich, I. Yu.
Pisman, T. I.
Shevyrnogov, A. P.] Siberian Fed Univ, RAS, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sid'ko, A.F.; Botvich, I.Y.; Pisman, T.I.; Shevyrnogov, A.P.

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Effect of laser-radiation polarization on the nonlinear scattering of light in nanodiamond suspensions [Text] / G. M. Mikheev [et al.] // Tech. Phys. Lett. - 2014. - Vol. 40, Is. 7. - P609-613, DOI 10.1134/S1063785014070220. - Cited References: 19. - This study was supported in part by the Russian Foundation for Basic Research (project no. 13-02-96016r_ural_a), the Presidium of the Ural Branch of the Russian Academy of Sciences (project no. 12-S-1-1003), and the Academy of Finland (grant no. 278252). . - ISSN 1063-7850. - ISSN 1090-6533

РУБ Physics, Applied
Рубрики:
CARBON NANOTUBE SUSPENSIONS
DETONATION NANODIAMOND
OPTICAL-PROPERTIES
Аннотация: The effect of laser radiation polarization on the nonlinear scattering of light in aqueous suspensions of detonation nanodiamonds (DNDs) in a regime of optical power limiting (OPL) has been studied. It is established that the nonlinear transmission coefficient of DND suspension in the OPL regime in a field of nanosecond laser pulses with a wavelength of 532 nm is independent of the polarization of incident radiation. The nonlinear scattering of light observed at an angle of 90A degrees in the plane perpendicular to the plane of polarization of the incident radiation depends on the polarization angle in accordance with a trigonometric law. It is shown that the ratio of the signals of scattered radiation for the vertical and horizontal polarizations exhibits nonmonotonic dependence on the laser-beam power density. The results are explained by the Rayleigh-Mie scattering and a change in the size of scattering centers as a result of the effect of a laser upon the DND suspension.

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Держатели документа:
[Mikheev, G. M.] Russian Acad Sci, Inst Mech, Ural Branch, Izhevsk 426067, Russia
Univ Eastern Finland, Inst Photon, FI-80101 Joensuu, Finland
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mikheev, G.M.; Vanyukov, V.V.; Mogileva, T.N.; Puzyr', A.P.; Bondar', V.S.; Svirko, Y.P.; Russian Foundation for Basic Research [13-02-96016r_ural_a]; Presidium of the Ural Branch of the Russian Academy of Sciences [12-S-1-1003]; Academy of Finland [278252]

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T-MATRIX APPROACH AND THE ANGULAR-MOMENTUM THEORY IN LIGHT-SCATTERING PROBLEMS BY ENSEMBLES OF ARBITRARILY-SHAPED PARTICLES [Text] / L. E. PARAMONOV // J. Opt. Soc. Am. A-Opt. Image Sci. Vis. - 1995. - Vol. 12, Is. 12. - P. 2698-2707, DOI 10.1364/JOSAA.12.002698. - Cited References: 38 . - ISSN 0740-3232

РУБ Optics
Рубрики:
ELECTROMAGNETIC SCATTERING
NONSPHERICAL GRAINS
EXTINCTION
Аннотация: Light scattering by a macroscopically anisotropic volume element, which consists of arbitrarily shaped particles with an arbitrary square-integrable probability-density function over orientations, is considered. The T-matrix approach and the quantum theory of angular momentum are used to develop a rigorous analytical method (in terms of the T matrix) for computing the extinction matrix; the extinction, scattering, and absorption cross sections; and the elements of the Mueller matrix of the volume element. The constructive theorem of the existence of the expansion of scattering-matrix elements in a series of Wigner functions for a medium with rotation symmetry is proved. The analytical results are generalized for incident radiation composed of incoherent plane waves with a square-integrable probability-density function over directions of propagation, intensities, and polarization states. (C) 1995 Optical Society of America.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
PARAMONOV, L.E.

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Polarization-sensitive nonlinear light scattering and optical limiting in aqueous suspension of detonation nanodiamonds [Text] / V. V. Vanyukov [et al.] // J. Opt. Soc. Am. B-Opt. Phys. - 2014. - Vol. 31, Is. 12. - P2990-2995, DOI 10.1364/JOSAB.31.002990. - Cited References: 29. - This work was supported by the Academy of Finland (grant 278252), the Russian Foundation for Basic Research (grant 13-02-96016 r_ural_a), the Emil Aaltosen Saatio foundation, the Erasmus staff exchange support (Dno 275/04.04.02.01), the Ural Branch of Russian Academy of Sciences (project 12-C-1-1003), and the FP7 Marie Curie projects CACOMEL and FANCEE. . - ISSN 0740-3224. - ISSN 1520-8540

РУБ Optics
Рубрики:
CARBON-BLACK SUSPENSIONS
   NANOTUBES
   DEPENDENCE
   MECHANISMS
   PARTICLES
   CLUSTERS
Аннотация: We report on the polarization dependence of the optical limiting and nonlinear light scattering in nanodiamond clusters suspended in distilled water. The nanosecond Z-scan measurements at a wavelength of 532 nm reveal that the nonlinear transmittance of the suspension is independent of polarization, while the energy of the light pulses scattered at 90 deg shows a cosine-like dependence on the polarization azimuth of the incident beam. Moreover, the ratio of the energies of the vertically and horizontally polarized scattered pulses is a nonmonotonous function of the input fluence. The obtained results can be explained in terms of the change of scattering center size under the laser action and are in agreement with the predictions of the Rayleigh-Mie scattering theory. (C) 2014 Optical Society of America

WOS
Держатели документа:
[Vanyukov, Viatcheslav V.
Svirko, Yuri P.] Univ Eastern Finland, Inst Photon, Joensuu 80101, Finland
[Mikheev, Gennady M.
Mogileva, Tatyana N.] Russian Acad Sci, Inst Mech, Izhevsk 426067, Russia
[Puzyr, Alexey P.
Bondar, Vladimir S.] Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vanyukov, V.V.; Mikheev, G.M.; Mogileva, T.N.; Puzyr, A.P.; Bondar, V.S.; Svirko, Y.P.; Academy of Finland [278252]; Russian Foundation for Basic Research [13-02-96016 r_ural_a]; Emil Aaltosen Saatio foundation; Erasmus staff exchange support [Dno 275/04.04.02.01]; Ural Branch of Russian Academy of Sciences [12-C-1-1003]; FP7 Marie Curie project CACOMEL; FP7 Marie Curie project FANCEE

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Nonequilibrium capillary electrophoresis of equilibrium mixtures, mathematical model [Text] / V. . Okhonin, S. M. Krylova, S. N. Krylov // Anal. Chem. - 2004. - Vol. 76, Is. 5. - P. 1507-1512, DOI 10.1021/ac035259p. - Cited References: 24 . - ISSN 0003-2700

РУБ Chemistry, Analytical
Рубрики:
SURFACE-PLASMON RESONANCE
   PROTEIN-DNA INTERACTIONS
   FLUORESCENCE POLARIZATION
   COMPLEX-FORMATION
   BINDING
   RECOGNITION
   DERIVATIVES
   TOOL
Аннотация: We recently introduced a new electrophoretic method, nonequilibrium, capillary electrophoresis of equilibrium mixtures (NECEEM). NECEEM provides a unique way of finding kinetic and equilibrium parameters of the formation of intermolecular complexes from a single electropherogram and allows for the use of weak affinity probes in protein quantitation. In this work, we study theoretical bases of NECEEM by developing a mathematical model for the new method. By solving a system of partial differential equations with diffusion in linear approximation, we found the analytical solution for concentrations of components involved in complex formation as functions of time from the beginning of separation and position in the capillary. The nonnumerical nature of the solution makes it a powerful tool in studying the theoretical foundations of the NECEEM method and modeling experimental results. We demonstrate the use of the model for finding binding parameters of complex formation by nonlinear regression of NECEEM electropherograms obtained experimentally.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Okhonin, V...; Krylova, S.M.; Krylov, S.N.

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Analysis of the polarization characteristics of wheat and maize crops using land-based remote-sensing measurements / A. F. Sid’ko [et al.] // Biophysics. - 2015. - Vol. 60, Is. 4. - P668-671, DOI 10.1134/S0006350915040223 . - ISSN 0006-3509
Кл.слова (ненормированные):
crops -- optical remote sensing techniques -- polarization characteristics
Аннотация: This paper presents an analysis of the study of the polarized component of the reflectance factor (Rq) and the degree of polarization (P) of wheat and maize crops depending on the wavelength. The polarization characteristics were obtained in the field from an elevated work platform at heights of 10 to 18 m in June and July. Measurements were performed using a double-beam spectrophotometer with a polarized light filter attachment within the spectral range from 400 to 820 nm. The viewing angle was no greater than 20° with respect to the nadir. The reflection spectra of wheat and maize crops that were obtained using a polarizer adjusted to transmit the maximum and minimum amounts of light (Rmax and Rmin) were studied. Based on these reflection spectra, the polarization characteristics, which differ in the visible and infrared spectral regions, were determined and analyzed. © 2015, Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Sid’ko, A. F.; Botvich, I. Y.; Pisman, T. I.; Shevyrnogov, A. P.

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Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients / N. G. Menzyanova [et al.] // Toxicol. Rep. - 2019. - Vol. 6. - P74-90, DOI 10.1016/j.toxrep.2018.11.009 . - ISSN 2214-7500
Кл.слова (ненормированные):
Atherosclerosis -- Cell morphology -- Intravascular stenting -- Macrophages -- Monocytes -- Polyhydroxyalkanoates
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients. © 2018 The Authors

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Держатели документа:
Siberian Federal University, 79, Svobodny av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Center for Cardiovascular Surgery, 45 Karaulnaya, Krasnoyarsk, 660020, Russian Federation

Доп.точки доступа:
Menzyanova, N. G.; Pyatina, S. А.; Nikolaeva, E. D.; Shabanov, A. V.; Nemtsev, I. V.; Stolyarov, D. P.; Dryganov, D. B.; Sakhnov, E. V.; Shishatskaya, E. I.

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