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Evidence of the radioactive fallout in the center of asia (russia) following the fukushima nuclear accident / A. Bolsunovsky, D. Dementyev // Journal of Environmental Radioactivity. - 2011. - Vol. 102, Is. 11. - P1062-1064, DOI 10.1016/j.jenvrad.2011.06.007 . - ISSN 0265-931X
Кл.слова (ненормированные):
Environmental samples -- Fission product radionuclides -- Fukushima nuclear accident -- Radioactive fallout -- Russia -- Chernobyl accident -- Environmental sample -- Fallout radionuclides -- Global effects -- High velocity -- Nuclear accidents -- Radioactive contamination -- Russia -- Water samples -- Cesium -- Fallout -- Fission products -- Radioactivity -- Radioisotopes -- Nuclear reactor accidents -- cesium 137 -- iodine 131 -- rain -- atmospheric pollution -- cesium isotope -- fallout -- nuclear accident -- radioactive pollution -- radionuclide -- article -- environmental exposure -- gamma spectrometry -- nuclear accident -- radioactive contamination -- radioactive waste -- water analysis -- water contamination -- Cesium Radioisotopes -- Cities -- Humans -- Iodine Radioisotopes -- Japan -- Radiation Dosage -- Radiation Monitoring -- Radioactive Fallout -- Radioactive Hazard Release -- Radioactive Pollutants -- Risk Assessment -- Russia -- Time Factors -- Fukushima -- Honshu -- Japan -- Krasnoyarsk [Russian Federation] -- Russian Federation -- Tohoku
Аннотация: It was recently reported that radioactive fallout due to the Fukushima Nuclear Accident was detected in environmental samples collected in the USA and Greece, which are very far away from Japan. In April-May 2011, fallout radionuclides ( 134Cs, 137Cs, 131I) released in the Fukushima Nuclear Accident were detected in environmental samples at the city of Krasnoyarsk (Russia), situated in the center of Asia. Similar maximum levels of 131I and 137Cs/ 134Cs and 131I/ 137Cs ratios in water samples collected in Russia and Greece suggest the high-velocity movement of the radioactive contamination from the Fukushima Nuclear Accident and the global effects of this accident, similar to those caused by the Chernobyl accident. В© 2011 Elsevier Ltd.

Scopus
Держатели документа:
Radioecology Laboratory, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 50-50, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Dementyev, D.

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Microdistribution of 241Am in structures of submerged macrophyte Elodea canadensis growing in the Yenisei River / L. Bondareva [et al.] // Journal of Environmental Radioactivity. - 2010. - Vol. 101, Is. 1. - P16-21, DOI 10.1016/j.jenvrad.2009.08.003 . - ISSN 0265-931X
Кл.слова (ненормированные):
Alpha-track analysis -- Americium -- Elodea canadensis -- Liquid-scintillation spectrometry -- Microdistribution -- Alpha-track analysis -- Elodea canadensis -- Microdistribution -- Scintillation spectrometry -- Track analysis -- Liquids -- Luminescence -- Radioisotopes -- Scintillation -- Spectrometry -- Spectroscopy -- Americium -- americium 241 -- americium -- americium -- bioaccumulation -- experimental study -- leaf -- macrophyte -- mass spectrometry -- morphology -- radionuclide -- spatial distribution -- stem -- submerged vegetation -- tracking -- aquatic flora -- article -- bioaccumulation -- concentration (parameters) -- elodea canadensis -- environmental radioactivity -- isotope analysis -- isotope tracing -- leaf lamina -- leaf surface -- macrophyte -- nonhuman -- plant cell -- plant morphology -- plant stem -- radioisotope distribution -- river -- chemistry -- Hydrocharitaceae -- metabolism -- plant -- plant leaf -- radiation monitoring -- river -- water pollutant -- Eurasia -- Russian Federation -- Yenisei River -- Elodea canadensis -- Americium -- Hydrocharitaceae -- Plant Leaves -- Plant Shoots -- Plant Stems -- Radiation Monitoring -- Rivers -- Water Pollutants, Radioactive
Аннотация: A submerged macrophyte of the Yenisei River, Elodea canadensis, was used to study the microdistribution of the artificial radionuclide 241Am among different components of the plant. The total amount of 241Am added to the experimental system was 1850 В± 31 Bq/L. The total amount of 241Am accumulated by the plants was 182 Bq per sample, or 758,333 В± 385 Bq/kg dry mass. It has been found that the major portion of 241Am accumulated by E. canadensis, up to 85%, was bound to solid components of the cells. It is observed that the microdistribution of 241Am within different components of the submerged plant E. canadensis was not uniform. 241Am distribution vary depending on the age of the leaf blades, the state of the cells and morphological features of the plant stem. В© 2009 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.; Vlasova, I.; Mogilnaya, O.; Bolsunovsky, A.; Kalmykov, S.

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The physicochemical properties of polyhydroxyalkanoates with different chemical structures / T. G. Volova [et al.] // Polymer Science - Series A. - 2013. - Vol. 55, Is. 7. - P427-437, DOI 10.1134/S0965545X13070080 . - ISSN 0965-545X
Кл.слова (ненормированные):
Amorphous and crystalline phasis -- Degrees of crystallinity -- Physicochemical property -- Poly(3-hydroxybutirate) -- Polyhydroxyalkanoate copolymer -- Temperature characteristic -- Thermal degradation temperatures -- Thermoplastic materials -- Copolymers -- Monomers -- Thermoplastic elastomers -- Structure (composition)
Аннотация: A set of polyhydroxyalkanoates are synthesized, and a comparative study of their physicochemical properties is performed. The molecular masses and polydispersities of polyhydroxyalkanoates are found to be independent of their chemical structures. It is shown that the temperature characteristics and degrees of crystallinity of polyhydroxyalkanoates are affected by the chemical compositions of the monomers and their quantitative contents in the polymers. The incorporation of 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate units into the chain of poly(3-hydroxybutyrate) decreases its melting point and thermal degradation temperature relative to these parameters of a homogeneous poly(3-hydroxybutyrate) sample (175 В± 5 C and 275 В± 5 C, respectively). The higher the content of the second monomer units in the poly(3-hydroxybutirate) chain, the greater the changes. The degrees of crystallinity of polyhydroxyalkanoate copolymers are generally lower than that of poly(3-hydroxybutyrate) (75 В± 5%). The effect on the ratio of the amorphous and crystalline phases of the copolymer samples becomes more pronounced in the series 3-hydroxy-valerate-3-hydroxyhexanoate-4- hydroxybutyrate. The prepared samples exhibit different properties ranging from rigid thermoplastic materials to engineering elastomers. В© 2013 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, str. 50, Krasnoyarsk 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk 660041, Russian Federation
Siberian State Technological University, pr. Mira 82, Krasnoyarsk 660049, Russian Federation
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, str. 38, Krasnoyarsk 660036, Russian Federation
Massachusetts Institute of Technology, Cambridge, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Shishatskaya, E.I.; Mironov, P.V.; Vasil'Ev, A.D.; Sukovatyi, A.G.; Sinskey, A.J.

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Lipid and hydrocarbon compositions of a collection strain and a wild sample of the green microalga Botryococcus / G. S. Kalacheva, N. O. Zhila, T. G. Volova // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P317-330, DOI 10.1023/A:1015615618420 . - ISSN 1386-2588
Кл.слова (ненормированные):
Batch culture -- Botoryococcus braunii -- Fatty acids -- Hydrocarbons -- Lipids -- chemical composition -- fatty acid -- green alga -- hydrocarbon -- lipid -- saline lake -- Russian Federation -- algae -- Bacteria (microorganisms) -- Botryococcus -- Botryococcus braunii -- Chlorophyta
Аннотация: Lipid composition and hydrocarbon structure of two colonial green algae of the genus Botryococcus, i.e., a mu seum strain and a field sample collected for the first time from Lake Shira (Khakasia, Siberia), have been compared. Polar lipids, diacylglycerols, alcohols, triacylglycerols, sterols, sterol esters, free fatty acids and hydrocarbons have been identified among lipids in the laboratory culture. The dominant fraction in the museum strain was formed by polar lipids (up to 50% of the lipids) made up of fatty acids from C12 to C24. Palmitic, oleic, C16 - C18 dienoic and trienoic acids were the main fatty acids of the museum strain. Aliphatic hydrocarbons were found in the lipid of the museum strain. However, these amounted maximally to about 1% of the dry biomass at the end of exponential growth phase. The qualitative and quantitative compositions of FAs and hydrocarbons of the museum strain of Botryococcus, (registered at the Cambridge collection as Botryococcus braunii Kutz No LB 807/1 Droop 1950 H-252) differed from those of the Botryococcus strain described in the literature as Botryococcus braunii. The Botryococcus sp. found in Lake Shira is characterized by a higher lipid content (< 40% of the dry weight). Polar lipids, sterols, triacylglycerols, free fatty acids and hydrocarbons have been identified among lipids in the field sample. The main lipids in this sample were dienes and trienes (hydrocarbons < 60% of total lipid). Monounsaturated and very long chain monounsaturated fatty acids, including C28:1 and C32:1 acids, were identified in the Botryococcus found in Lake Shira. The chemo-taxonomic criteria allow us to unequivocally characterize the organism collected from Lake Shira as Botryococcus braunii race A.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zhila, N.O.; Volova, T.G.

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Effect of boiling and frying on the content of essential polyunsaturated fatty acids in muscle tissue of four fish species / M. I. Gladyshev [et al.] // Food Chemistry. - 2007. - Vol. 101, Is. 4. - P1694-1700, DOI 10.1016/j.foodchem.2006.04.029 . - ISSN 0308-8146
Кл.слова (ненормированные):
Cod -- Essential polyunsaturated fatty acids -- Herring -- Sole -- Trout -- docosahexaenoic acid -- icosapentaenoic acid -- polyunsaturated fatty acid -- article -- Atlantic cod -- Atlantic herring -- boiling point -- brown trout -- controlled study -- cooking -- fish -- food processing -- frying -- Lepidopsetta bilineata -- muscle tissue -- nonhuman -- Norway -- raw meat -- Russian Federation -- sample -- Clupea pallasi -- Clupeidae -- Gadus ogac -- Lepidopsetta bilineata -- Martes pennanti -- Paraplagusia bilineata -- Salmo trutta -- Salmonidae
Аннотация: Frozen samples of common fish species, sea trout (Salmo trutta), from Norway and Siberia, herring (Clupea harengus pallasi), rock sole (Lepidopsetta bilineata) and cod (Gadus morhua maris-albi), collected from a wholesale market in Krasnoyarsk city (Siberia, Russia) were analyzed. Special attention was paid to long-chain essential polyunsaturated fatty acids: eicosapentaenoic, 20:5?3 (EPA) and docosahexaenoic, 22:6?3 (DHA). Heat-treatment (cooking and frying) did not in general significantly decrease the contents of EPA and DHA compared to raw fish species, except for a modest reduction in Norwegian trout during frying. Boiled trout appeared to be a more valuable fish dish for obtaining the officially recommended appropriate daily intake of EPA + DHA for humans. Herring and sole had intermediate values, while boiled cod had a comparatively low value. В© 2006 Elsevier Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Krasnoyarsk State Trade-Economical Institute, Lidiya Prushinskaya Street, 2, Krasnoyarsk 660075, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Gubanenko, G.A.; Demirchieva, S.M.; Kalachova, G.S.

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A Highly Sensitive and Rapid Method for the Detection of DNA Fragments Using the Photoprotein Obelin as a Reporter [Text] / V. V. Borisova [et al.] // Russ. J. Bioorg. Chem. - 2008. - Vol. 34, Is. 6. - P709-715, DOI 10.1134/S1068162008060101. - Cited References: 13. - This work was supported the program Molecular and Cellular Biology (project no. 10.6), integration grants of the Siberian Division of the Russian Academy of Sciences (73 and 55), CRDF, and the Russian Foundation for Basic Research (project nos. 06-04-49263-a and 06-04-08076-ofi). . - ISSN 1068-1620

РУБ Biochemistry & Molecular Biology + Chemistry, Organic
Рубрики:
BIOLUMINESCENT IMMUNOASSAY
Кл.слова (ненормированные):
obelin -- bioluminescent hybridization assay -- PCR
Аннотация: The recombinant Ca(2+)-activated photoprotein obelin was used as a reporter protein in a solid-phase bioluminescent hybridization DNA assay. Oligonucleotide probes were immobilized on the surface of polymer methacrylate beads or microbiological plates of different types. A 30-mer oligonucleotide or its derivative with the biotin residue on the 3'-terminus, as well as a denatured double-stranded PCR fragment of the hepatitis C virus with the sequence of the 30-mer oligonucleotide was used as a DNA template. The probe in the hybridization complex was labeled by the elongation of the chain using a Taq DNA polymerase in the presence of biotinylated deoxyuridine triphosphate. The results of the bioluminescent assay were compared with the results of colorimetric analysis obtained with alkaline phosphatase as a reporter protein. It was shown that the use of the bioluminescent obelin label substantially accelerates the DNA detection procedure, provides a high sensitivity of the assay (no less than 10(-15) mol of DNA template), and ensures a quantitative determination of the amount of DNA template in the tested sample.

Держатели документа:
[Borisova, V. V.
Frank, L. A.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Pyshnaya, I. A.
Pyshnyi, D. V.] Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Borisova, V.V.; Pyshnaya, I.A.; Pyshnyi, D.V.; Frank, L.A.

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Flocculation of bacterial cells in a culture with protozoans [Текст] / Y. L. Gurevich, M. I. Teremova, A. V. Latyntseva // Izv. Akad. Nauk Ser. Biol. - 1998. - Is. 3. - P. 351-355. - Cited References: 13 . - ISSN 0002-3329

РУБ Biology

Аннотация: The initial phase of formation of the biogenic suspension was studied in experimental communities of bacteria and protozoans that simulate degradation of the phenol technogenic flows. Protozoans were shown to initiate formation of bacterial aggregates and increase the size of bacterial flocules several hundred times. Factors were found by the methods of mathematical planning, which markedly affect the size and amount of flocules. Unlike natural water bodies, in the sample communites, the presence of abiogenic substrate was not essential for aggregation of the bacteria, The aggregated bacterial cells make an important contribution to degradation of organic compounds.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gurevich, Y.L.; Teremova, M.I.; Latyntseva, A.V.

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Application of Enzyme Bioluminescence in Ecology [Text] / E. Esimbekova, V. Kratasyuk, O. Shimomura // Adv. Biochem. Eng. Biotechnol. : SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - P67-109. - (Advances in Biochemical Engineering-Biotechnology), DOI 10.1007/978-3-662-43385-0_3. - Cited References:85 . -

РУБ Biotechnology & Applied Microbiology
Рубрики:
BACTERIAL LUCIFERASE
   IN-VITRO
   PYRETHROID INSECTICIDES
   FRESH-WATER
Кл.слова (ненормированные):
Bioluminescence -- Ecological monitoring -- Enzymatic assay -- Immobilization -- Integral water toxicity -- Luciferase
Аннотация: This review examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent enzyme system technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH: FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multicomponent reagent "Enzymolum'' has been developed, which contains the bacterial luciferase, NADH: FMN-oxidoreductase, and their substrates, co-immobilized in starch or gelatin gel. Enzymolum is the central part of Portable Laboratory for Toxicity Detection (PLTD), which consists of a biodetector module, a sampling module, a sample preparation module, and a reagent module. PLTD instantly signals chemical-biological hazards and allows us to detect a wide range of toxic substances. Enzymolum can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of Enzymolum and the algorithm for creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. Possible applications of enzyme bioassays are seen in the examples for medical diagnostics, assessment of the effect of physical load on sportsmen, analysis of food additives, and in practical courses for higher educational institutions and schools. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 min), high sensitivity, simplicity and safety of procedure, and possibility of automation of ecological monitoring; the required luminometer is easily available.

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Держатели документа:
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Esimbekova, Elena; Kratasyuk, Valentina; Shimomura, Osamu

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Near-IR nonlinear optical filter for optical communication window [Text] / V. V. Vanyukov [et al.] // Appl. Optics. - 2015. - Vol. 54, Is. 11. - P3290-3293, DOI 10.1364/AO.54.003290. - Cited References:21. - This work was supported by the Academy of Finland (grants no. 278252 and no. 288547), the Emil Aaltosen Saatio foundation, Russian Foundation for Basic Research (project no. 13-02-96016 r_ural_a), the Erasmus staff exchange support (Dno 275/04.04.02.01), and FP7 Marie Curie project FANCEE. . - ISSN 1559-128X. - ISSN 2155-3165

РУБ Optics
Рубрики:
DETONATION NANODIAMOND CLUSTERS
LIGHT-SCATTERING
SUSPENSIONS
Аннотация: We report on a high performance nonlinear optical filter for the telecommunication window that employs detonation nanodiamonds (NDs). The nanosecond Z-scan experiments revealed that the heavy water ND suspensions enable strong optical limiting in the wavelength range of 1400-1675 nm. We observed an enhancement of the optical limiting performance in the blue part of the communication window. In particular, at the wavelength of 1400 nm the transmittance of the 2 mm thick sample with 4.5 wt. % ND concentration is suppressed by 45% for the input fluence of 3.8 J/cm(2). The proposed nonlinear optical filter employs the phenomena of the nonlinear absorption and the nonlinear light scattering in ND suspensions. (C) 2015 Optical Society of America

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Scopus
Держатели документа:
Univ Eastern Finland, Inst Photon, Joensuu, Finland.
Russian Acad Sci, Inst Mech, Izhevsk, Russia.
Russian Acad Sci, Inst Biophys, Krasnoyarsk, Russia.

Доп.точки доступа:
Vanyukov, Viatcheslav V.; Mikheev, Gennady M.; Mogileva, Tatyana N.; Puzyr, Alexey P.; Bondar, Vladimir S.; Svirko, Yuri P.; Academy of Finland [278252, 288547]; Emil Aaltosen Saatio foundation; Russian Foundation for Basic Research [13-02-96016 r_ural_a]; Erasmus staff exchange support [Dno 275/04.04.02.01]; FP7 Marie Curie project FANCEE

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10.

Mitrocomin from the jellyfish Mitrocoma cellularia with deleted C-terminal tyrosine reveals a higher bioluminescence activity compared to wild type photoprotein / L. P. Burakova [et al.] // J. Photochem. Photobiol. B Biol. - 2016. - Vol. 162. - P286-297, DOI 10.1016/j.jphotobiol.2016.06.054 . - ISSN 1011-1344
Аннотация: The full-length cDNA genes encoding five new isoforms of Ca2 +-regulated photoprotein mitrocomin from a small tissue sample of the outer bell margin containing photocytes of only one specimen of the luminous jellyfish Mitrocoma cellularia were cloned, sequenced, and characterized after their expression in Escherichia coli and subsequent purification. The analysis of cDNA nucleotide sequences encoding mitrocomin isoforms allowed suggestion that two isoforms might be the products of two allelic genes differing in one amino acid residue (64R/Q) whereas other isotypes appear as a result of transcriptional mutations. In addition, the crystal structure of mitrocomin was determined at 1.30 A resolution which expectedly revealed a high similarity with the structures of other hydromedusan photoproteins. Although mitrocomin isoforms reveal a high degree of identity of amino acid sequences, they vary in specific bioluminescence activities. At that, all isotypes displayed the identical bioluminescence spectra (473–474 nm with no shoulder at 400 nm). Fluorescence spectra of Ca2 +-discharged mitrocomins were almost identical to their light emission spectra similar to the case of Ca2 +-discharged aequorin, but different from Ca2 +-discharged obelins and clytin which fluorescence is red-shifted by 25–30 nm from bioluminescence spectra. The main distinction of mitrocomin from other hydromedusan photoproteins is an additional Tyr at the C-terminus. Using site-directed mutagenesis, we showed that this Tyr is not important for bioluminescence because its deletion even increases specific activity and efficiency of apo-mitrocomin conversion into active photoprotein, in contrast to C-terminal Pro of other photoproteins. Since genes in a population generally exist as different isoforms, it makes us anticipate the cloning of even more isoforms of mitrocomin and other hydromedusan photoproteins with different bioluminescence properties. © 2016 Elsevier B.V.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, China
iHuman Institute, ShanghaiTech University, Shanghai, China

Доп.точки доступа:
Burakova, L. P.; Natashin, P. V.; Markova, S. V.; Eremeeva, E. V.; Malikova, N. P.; Cheng, C.; Liu, Z. -J.; Vysotski, E. S.

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11.

Luminescence of wood samples during long-term storage / A. P. Puzyr, S. E. Medvedeva // Mycosphere. - 2016. - Vol. 7, Is. 6. - P716-727, DOI 10.5943/mycosphere/7/6/2 . - ISSN 2077-7000
Кл.слова (ненормированные):
Light emitting wood -- Luminous mycelia
Аннотация: The present study describes changes in the mycelium of the fungus growing on the luminescent wood collected on Borneo Island in early December 2013 that occurred during 31 months of storage. The study shows that wood samples retain their ability to emit light, forming two types of luminescent mycelium: surface mycelium and aerial mycelium. The hyphae of the surface mycelium form on the surface of the wood sample and then spread over the surface of the polyethylene bag or over the surface of the bottom of tissue culture flasks containing the samples. The aerial mycelium develops later and only in tissue culture flasks, forming biomass composed of local interlaced hyphae, growing upward. The surface mycelium is characterized by non-uniform "flickering" luminescence along the hyphae. There is no diurnal periodicity in the luminescence of this fungus, but luminescence is increased by mechanical disturbance or exposure to ultraviolet radiation. The local impact of these factors causes an increase in luminescence of the mycelium regions that have not been directly affected. It has been assumed that the variable level of luminescence is an individual trait of this fungus species. The results obtained in this study suggest that luminescent wood found on Borneo Island contains mycelium of a fungus species, whose luminescent properties are essentially different from those of the fungi described in the scientific literature.

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Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Science, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Puzyr, A. P.; Medvedeva, S. E.

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12.

Mutants of Ca2+-regulated Photoprotein Obelin for Site-specific Conjugation / V. V. Krasitskaya [et al.] // Photochem. Photobiol. - 2017. - Vol. 93, Is. 2. - P553-557, DOI 10.1111/php.12712 . - ISSN 0031-8655
Аннотация: Color variants of Ca2+-regulated photoprotein obelin were shown to be an important tool for dual-analyte binding assay. To provide site-directed conjugation with biospecific molecules, several obelin color mutants carrying unique cysteine residues were obtained and characterized for their novel properties. A pair of obelins Y138F,A5C and W92F,H22E,D12C was found to be most suitable (in terms of high bioluminescent activity and stability) as reporters in simultaneous assay of two targets in a sample. Availability of SH-groups, accessible for chemical modification, essentially simplifies the synthesis of biospecific conjugates, increases their yield and conserves obelins' bioluminescence activity. Conjugates with immunoglobulin and oligonucleotide were produced and successfully applied in single nucleotide polymorphism genotyping. © 2017 The American Society of Photobiology

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WOS
Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Krasitskaya, V. V.; Burakova, L. P.; Komarova, A. A.; Bashmakova, E. E.; Frank, L. A.

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13.

Active mixing of immobilised enzymatic system in microfluidic chip / K. A. Lukyanenko [et al.] // Micro Nano Lett. - 2017. - Vol. 12, Is. 6. - P377-381, DOI 10.1049/mnl.2016.0646. - Cited References:17. - The research was supported by the grant of the Russian Science Foundation (project no. 15-19-10041). . - ISSN 1750-0443

РУБ Nanoscience & Nanotechnology + Materials Science, Multidisciplinary
Рубрики:
POLY(METHYL METHACRYLATE)
   SURFACE MODIFICATION
   POINT
   DEVICES
   PMMA
Аннотация: Parameters for sample introduction, dried reagents dissolution and mixing with sample for bienzyme system NAD(H):FMN-oxidoreductase and luciferase immobilised in microfluidic chip were successfully determined. Numerical simulations of reaction chamber geometry, flavin mononucleotide (FMN) escape from starch gel and mixing options were conducted to achieve higher sensitivity of bioluminescent reaction. Results of numerical simulations were verified experimentally. The active mixer for dried reagents was made from an electro-mechanical speaker's membrane which was connected to the input of the chip. Such a mixer provided better efficiency than a passive mixing, and it is simple enough for use in point-of-care devices with any systems based on immobilised enzymes in chips.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ITMO Univ, St Petersburg 197101, Russia.
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Inst Analyt Instrumentat, St Petersburg 198095, Russia.

Доп.точки доступа:
Lukyanenko, Kirill A.; Belousov, Kirill I.; Denisov, Ivan A.; Yakimov, Anton S.; Esimbekova, Elena N.; Bukatin, Anton S.; Evstrapov, Anatoly A.; Belobrov, Peter I.; Russian Science Foundation [15-19-10041]

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14.

Detection of nanodiamonds in biological samples by EPR spectrometry / E. V. Inzhevatkin [et al.] // Doklad. Biochem. Biophys. - 2017. - Vol. 477, Is. 1. - P394-397, DOI 10.1134/S1607672917060138 . - ISSN 1607-6729
Аннотация: In model experiments in vitro, the applicability of the EPR spectrometry method for the detection of modified nanodiamonds (MNDs) in blood and homogenates of mouse organs has been established. A characteristic signal (g = 2.003, ?H ? 10 G) is observed in the samples of biomaterials containing MNDs, the intensity of which increases linearly with the concentration of nanoparticles in the range of 1.6–200 ?g MNDs per 1 mL of the sample. The EPR method in biomaterials reveals the presence of intrinsic paramagnetic centers, signals from which are superimposed on the signal from the MNDs. However, the intensity of these signals is small, which makes it possible to register the MNDs using EPR spectrometry with the necessary accuracy. The data obtained open up the prospects of using the EPR method for studies of the interorgan distribution, accumulation, and elimination of MNDs during their intravenous injection into experimental animals. © 2017, Pleiades Publishing, Ltd.

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Держатели документа:
International Scientific Center for Studies of Extreme States of Organism, Federal Research Center “Krasnoyarsk Scientific Center,” Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center”, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology, Federal Research Center “Krasnoyarsk Scientific Center,” Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Inzhevatkin, E. V.; Baron, A. V.; Maksimov, N. G.; Volkova, M. B.; Puzyr, A. P.; Bondar, V. S.

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15.

Comprehensive Insights Into Composition, Metabolic Potentials, and Interactions Among Archaeal, Bacterial, and Viral Assemblages in Meromictic Lake Shunet in Siberia / Y. T. Wu [et al.] // Front. Microbiol. - 2018. - Vol. 9. - Ст. 1763, DOI 10.3389/fmicb.2018.01763. - Cited References:103. - This research was supported by the Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project Grant NSC 99-2923-B-001-001-MY3 and NSC 102-2923-B-001-004 and the Russian Foundation for Basic Research, Grant No. 16-05-00091-a. The research was partially supported by the Council on grants from the President of the Russian Federation for support of leading scientific schools (grant NSh-9249.2016.5). . - ISSN 1664-302X

РУБ Microbiology
Рубрики:
BACTERIOPLANKTON COMMUNITY COMPOSITION
RIBOSOMAL-RNA GENES
ANTARCTIC
Кл.слова (ненормированные):
Lake Shunet -- metagenomics -- archaeal -- bacterial and viral assemblages -- meromictic lake
Аннотация: Microorganisms are critical to maintaining stratified biogeochemical characteristics in meromictic lakes; however, their community composition and potential roles in nutrient cycling are not thoroughly described. Both metagenomics and metaviromics were used to determine the composition and capacity of archaea, bacteria, and viruses along the water column in the landlocked meromictic Lake Shunet in Siberia. Deep sequencing of 265 Gb and high-quality assembly revealed a near-complete genome corresponding to Nonlabens sp. sh3vir. in a viral sample and 38 bacterial bins (0.2-5.3 Mb each). The mixolimnion (3.0 m) had the most diverse archaeal, bacterial, and viral communities, followed by the monimolimnion (5.5 m) and chemocline (5.0 m). The bacterial and archaeal communities were dominated by Thiocapsa and Methanococcoides, respectively, whereas the viral community was dominated by Siphoviridae. The archaeal and bacterial assemblages and the associated energy metabolism were significantly related to the various depths, in accordance with the stratification of physicochemical parameters. Reconstructed elemental nutrient cycles of the three layers were interconnected, including co-occurrence of denitrification and nitrogen fixation in each layer and involved unique processes due to specific biogeochemical properties at the respective depths. According to the gene annotation, several pre-dominant yet unknown and uncultured bacteria also play potentially important roles in nutrient cycling. Reciprocal BLAST analysis revealed that the viruses were specific to the host archaea and bacteria in the mixolimnion. This study provides insights into the bacterial, archaeal, and viral assemblages and the corresponding capacity potentials in Lake Shunet, one of the three meromictic lakes in central Asia. Lake Shunet was determined to harbor specific and diverse viral, bacterial, and archaeal communities that intimately interacted, revealing patterns shaped by indigenous physicochemical parameters.

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Держатели документа:
Natl Pingtung Univ Sci & Technol, Dept Forestry, Neipu, Taiwan.
Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan.
Germark Biotechnol Co Ltd, Taichung, Taiwan.
Univ Melbourne, Melbourne Sch Engn, Optimisat & Pattern Recognit Grp, Melbourne, Vic, Australia.
Acad Sinica, Taiwan Int Grad Program, Mol & Biol Agr Sci, Taipei, Taiwan.
Natl Chung Hsing Univ, Grad Inst Biotechnol, Dept Life Sci, Taichung, Taiwan.
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Wu, Yu-Ting; Yang, Cheng-Yu; Chiang, Pei-Wen; Tseng, Ching-Hung; Chiu, Hsiu-Hui; Saeed, Isaam; Baatar, Bayanmunkh; Rogozin, Denis; Halgamuge, Saman; Degermendzhi, Andrei; Tang, Sen-Lin; Ministry of Science and Technology in Taiwan [NSC 99-2923-B-001-001-MY3, NSC 102-2923-B-001-004]; Russian Foundation for Basic Research [16-05-00091-a]; Russian Federation [NSh-9249.2016.5]

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16.

Disposable luciferase-based microfluidic chip for rapid assay of water pollution / I. Denisov [et al.] // Lumin. - 2018. - Vol. 33, Is. 6. - P1054-1061, DOI 10.1002/bio.3508 . - ISSN 1522-7235
Кл.слова (ненормированные):
bioassay -- lab-on-a-chip -- luciferase -- microfluidics -- solvent bonding
Аннотация: In the present study, we demonstrate the use of a disposable luciferase-based microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve after the introduction of the water sample and thus activate bioluminescent reactions. Limits of detection for copper (II) sulfate, 1,3-dihydroxybenzene and 1,4-benzoquinone for the proposed microfluidic biosensor measured 3 ?M, 15 mM, and 2 ?M respectively, and these values are higher or close to the level of conventional environmental biosensors based on lyophilized bacteria. Approaches for entrapment of enzymes on poly(methyl methacrylate) (PMMA) plates using a gelatin scaffold and solvent bonding of PMMA chip plates under room temperature were suggested. The proposed microfluidic system may be used with some available luminometers and future portable luminescence readers. © 2018 John Wiley & Sons, Ltd.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS Federal Research Center'Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Denisov, I.; Lukyanenko, K.; Yakimov, A.; Kukhtevich, I.; Esimbekova, E.; Belobrov, P.

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17.

Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients / N. G. Menzyanova [et al.] // Toxicol. Rep. - 2019. - Vol. 6. - P74-90, DOI 10.1016/j.toxrep.2018.11.009 . - ISSN 2214-7500
Кл.слова (ненормированные):
Atherosclerosis -- Cell morphology -- Intravascular stenting -- Macrophages -- Monocytes -- Polyhydroxyalkanoates
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients. © 2018 The Authors

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Держатели документа:
Siberian Federal University, 79, Svobodny av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Center for Cardiovascular Surgery, 45 Karaulnaya, Krasnoyarsk, 660020, Russian Federation

Доп.точки доступа:
Menzyanova, N. G.; Pyatina, S. А.; Nikolaeva, E. D.; Shabanov, A. V.; Nemtsev, I. V.; Stolyarov, D. P.; Dryganov, D. B.; Sakhnov, E. V.; Shishatskaya, E. I.

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18.

The Influence of Temperature and Humidity on Greenhouse Gas Emission in Experiments on Imitation of the Full Vegetation Cycle of Tundra Ecosystems / Y. V. Barkhatov [et al.] // Dokl. Earth Sci. - 2018. - Vol. 483, Is. 2. - P1539-1541, DOI 10.1134/S1028334X18120115. - Cited References:15. - This study was supported by the Russian Foundation for Basic Research, the Krasnoyarsk Krai Government and the Krasnoyarsk Regional Fund of Science (project no. 17-45-240884), the Russian Foundation for Basic Research (project no. 16-04-01677-a), and the Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, governmental assignment, theme no. 56.1.4. for 2013-2020. . - ISSN 1028-334X. - ISSN 1531-8354

РУБ Geosciences, Multidisciplinary
Рубрики:
POLYGONAL TUNDRA
   METHANE EMISSION
   CARBON
   DELTA
   LAKES
Аннотация: Laboratory experiments were conducted in a hermetically sealed growth chamber with two soil samples obtained from the arctic tundra zone with different levels of moisture. Samples were maintained at a growing season typical of the region from which they were taken, and for the sample with a high level of moisture it was made twice: with the temperature in accord with natural conditions and one increased by 2 degrees C. It has been shown that heating of the overmoistened tundra soil by 2 degrees C can increased the average carbon dioxide emissions by almost two times (from 75 to 100-150 mg m(-2) h(-1)). Upon the application of heat, no significant increase in methane emission was observed.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Sukachev Inst Forests, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Barkhatov, Yu. V.; Ushakova, S. A.; Shikhov, V. N.; Evgrafova, S. Yu.; Tikhomirov, A. A.; Degermendzhi, A. G.; Russian Foundation for Basic Research; Krasnoyarsk Krai Government; Krasnoyarsk Regional Fund of Science [17-45-240884]; Russian Foundation for Basic Research [16-04-01677-a]; Institute of Biophysics, Siberian Branch, Russian Academy of Sciences [56.1.4.]

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19.

Seasonal variability of length-weight relationships of Arctic grayling (Thymallus arcticus) and Siberian dace (Leuciscus baicalensis) inhabiting the middle reaches of the Yenisei River, Siberia, Russia / I. V. Zuev, E. A. Trofimova, T. A. Zotina // Turk. J. Fish. Quat. Sci. - 2019. - Vol. 19, Is. 10. - P893-897, DOI 10.4194/1303-2712-v19_10_09. - Cited References:23. - The authors would like to thank Natalia Oskina, Nikolay Moshkin, and Tatiana Fetisova for their help with fish measurements. The work was partly supported by Russian Foundation for Basic Research, Grant No. 18-44-240003. . - ISSN 1303-2712. - ISSN 2149-181X

РУБ Fisheries + Marine & Freshwater Biology
Рубрики:
FISH
GROWTH
PALLAS
Кл.слова (ненормированные):
LWR -- Fish condition -- Baikal grayling -- Total length -- Total body weight
Аннотация: The present study proves the presence of seasonal variability of LWRs of Arctic grayling and Siberian dace inhabiting the middle reaches of the Yenisei River. LWRs were estimated using total length (cm) and total body weight (g). The literature data on LWRs of dace and grayling from different regions were compared using log a over b plot. The comparison revealed that seasonal variability of LWRs was wider than the geographic variability for these species. The position of points on the plot can be used to predict the season of sample collection and vice versa.

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Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Svobodny Av 79, Krasnoyarsk 660041, Russia.
RAS, SB, Fed Res Ctr, Krasnoyarsk Sci Ctr,Inst Biophys, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zuev, Ivan V.; Trofimova, Elena A.; Zotina, Tatiana A.; Zuev, Ivan; Russian Foundation for Basic Research [18-44-240003]

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20.

Development and characterization of novel 2 '-F-RNA aptamers specific to human total and glycated hemoglobins / A. Davydova [et al.] // Anal. Biochem. - 2019. - Vol. 570. - P43-50, DOI 10.1016/j.ab.2019.02.004. - Cited References:32. - We want to thank Dr. Alexander Lomzov (ICBFM SB RAS) for his valuable assistance with circular dichroism studies. The work was supported by the Russian Science Foundation (grant number 16-14-10296). . - ISSN 0003-2697. - ISSN 1096-0309

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology
Рубрики:
RNA APTAMER
   SEQUENCES
   PROTEIN
   DNA
Аннотация: Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2'-F-RNA aptamers against total human hemoglobin or its glycated form HbA1c. After SELEX and high-throughput sequencing of the enriched libraries, affinities and specificities of candidate aptamers and their truncated variants were examined by the solid-phase bioluminescent assay. As a result, we identified aptamers specific to both hemoglobins or only glycated HbA1c. The developed 2'-F-RNA aptamers have shown their applicability for detection of total and glycated hemoglobin in one sample.

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Держатели документа:
Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk, Russia.
Novosibirsk State Univ, Dept Nat Sci, Pirogova St 2, Novosibirsk 630090, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Davydova, Anna; Vorobyeva, Mariya; Bashmakova, Eugenia; Vorobjev, Pavel; Krasheninina, Olga; Tupikin, Alexey; Kabilov, Marsel; Krasitskaya, Vasilisa; Frank, Ludmila; Venyaminova, Alya; Krasitskaya, Vasilisa V; Frank, Ludmila A; Russian Science Foundation [16-14-10296]

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