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Degradation of P(3HB) and P(3HB-co-3HV) in biological media / E. I. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - P643-657, DOI 10.1163/1568562053783678 . - ISSN 0920-5063
Кл.слова (ненормированные):
Degradation rate -- Fiber properties -- Morphology -- Poly(hydroxybutyrate-co-hydroxyvalerate) (P(3HB-co-3HV)) -- Polyhydroxyalkanoates(PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Copolymers -- Degradation -- Differential scanning calorimetry -- Enzymes -- Morphology -- Scanning electron microscopy -- Tensile strength -- Tissue -- Transmission electron microscopy -- Degradation rate -- Fiber properties -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Biopolymers -- buffer -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxybutyrate hydroxyvalerate copolymer -- unclassified drug -- animal experiment -- animal model -- animal tissue -- article -- biodegradation -- controlled study -- crystal structure -- fiber -- giant cell -- in vitro study -- in vivo study -- macrophage -- morphology -- nonhuman -- pH -- priority journal -- rat -- structure analysis -- tensile strength -- tissue water -- weight reduction -- Animals -- Biodegradation, Environmental -- Buffers -- Humans -- Hydrogen-Ion Concentration -- Hydroxybutyrates -- Macrophages -- Microscopy, Electron, Scanning -- Microscopy, Electron, Transmission -- Muscle, Skeletal -- Polyesters -- Rats -- Rats, Wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 600326, Russian Federation
Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G3, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gordeev, S.A.; Puzyr, A.P.

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Effects of Modified Detonation Nanodiamonds on the Biochemical Composition of Human Blood / A. V. Baron [et al.] // Bull. Exp. Biol. Med. - 2013. - Vol. 154, Is. 6. - P781-784, DOI 10.1007/s10517-013-2055-y. - Cited References: 14 . - ISSN 0007-4888

РУБ Medicine, Research & Experimental
Рубрики:
PARTICLES
Кл.слова (ненормированные):
modified nanodiamonds -- blood serum
Аннотация: In vitro experiments showed that protein and non-protein components of human blood serum could be absorbed on the surface of modified nanodiamonds obtained by detonation synthesis. The prospects of using nanodiamond as a new absorbent for hemodialysis, plasmapheresis, and laboratory diagnostics are discussed.

Держатели документа:
[Baron, A. V.] Russian Acad Sci, Siberian Branch, Krasnoyarsk Res Ctr, Krasnoyarsk, Russia
[Puzyr, A. P.
Bondar, V. S.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Baron, I. I.] VF Voino Yasenetskii Krasnoyarsk State Med Univ, Krasnoyarsk, Russia
ИБФ СО РАН
КНЦ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Baron, A.V.; Puzyr, A.P.; Baron, I.I.; Bondar, V.S.

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Nanodiamonds as Carriers for Address Delivery of Biologically Active Substances [Text] / K. V. Purtov [et al.] // Nanoscale Res. Lett. - 2010. - Vol. 5, Is. 3. - P631-636, DOI 10.1007/s11671-010-9526-0. - Cited References: 24. - This work was supported by the Program # 27 for Basic Research of the Presidium of RAS (project 3.6.3). . - ISSN 1931-7573

РУБ Nanoscience & Nanotechnology + Materials Science, Multidisciplinary + Physics, Applied
Рубрики:
ANTICANCER DRUGS
   NANOPARTICLES
   ADSORPTION
   PARTICLES
   PROTEINS
Кл.слова (ненормированные):
Nanodiamonds -- Ligand -- Protein immobilization -- Nanocarrier -- Targeted delivery
Аннотация: Surface of detonation nanodiamonds was functionalized for the covalent attachment of immunoglobulin, and simultaneously bovine serum albumin and Rabbit Anti-Mouse Antibody. The nanodiamond-IgG(I125) and RAM-nanodiamond-BSA(I125) complexes are stable in blood serum and the immobilized proteins retain their biological activity. It was shown that the RAM-nanodiamond-BSA(I125) complex is able to bind to the target antigen immobilized on the Sepharose 6B matrix through antibody-antigen interaction. The idea can be extended to use nanodiamonds as carriers for delivery of bioactive substances (i.e., drugs) to various targets in vivo.

Держатели документа:
[Purtov, K. V.
Puzyr, A. P.
Bondar, V. S.] SB RAS, Inst Biophys, Krasnoyarsk, Russia
[Petunin, A. I.] Med Res Co Dias, Krasnoyarsk, Russia
[Burov, A. E.] SB RAS, Inst Computat Modeling, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Purtov, K.V.; Petunin, A.I.; Burov, A.E.; Puzyr, A.P.; Bondar, V.S.

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Simultaneous Bioluminescent Immunoassay of Serum Total and IgG-Bound Prolactins / A. N. Kudryavtsev [et al.] // Anal. Chem. - 2012. - Vol. 84, Is. 7. - P3119-3124, DOI 10.1021/ac300444w. - Cited References: 10. - This work was supported in part by Grant No. 76 of the Russian Academy of Sciences, Siberian Branch and by the Program of the Government of Russian Federation "Measures to attract leading scientists to Russian educational institutions" (Grant No 11. G34.31.058). . - ISSN 0003-2700

РУБ Chemistry, Analytical
Рубрики:
PHOTOPROTEIN OBELIN
   POLYETHYLENE-GLYCOL
   MACROPROLACTINEMIA
   PRECIPITATION
   VALIDATION
Аннотация: Novel dual-analyte single-well bioluminescence immunoassay (BLIA) for total and IgG-bound prolactins was developed on the base of Ca2+-regulated photoprotein obelin mutants with altered color and kinetics of bioluminescence signal as reporters. The mutants W92F-H22E and Y138F were chemically conjugated with monoclonal mouse anti-hPRL and anti-hIgG immunoglobulins and thus displayed signals from total prolactin and IgG-bounded prolactin (macroprolactin) correspondingly. Bioluminescence of the reporters was simultaneously triggered by a single injection of Ca2+ solution and discriminated via bioluminescent signal spectral and time resolution. The developed microplate-based immunoassay allows detection of two prolactin forms in crude serum without additional manipulations (e.g., gel chromatography or PEG-precipitation). Total prolactin bioluminescence immunoassay in standard, control, and clinical sera offers high sensitivity and reproducibility. The BLIA results show good correlation with those obtained by RIA and immunoassay after gel chromatography.

Держатели документа:
[Kudryavtsev, Alexander N.
Krasitskaya, Vasilisa V.
Frank, Ludmila A.] Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk 660036, Russia
[Kudryavtsev, Alexander N.
Krasitskaya, Vasilisa V.
Frank, Ludmila A.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Petunin, Alexei I.] DIAS Ltd, Krasnoyarsk 660036, Russia
[Burakov, Andrey Y.] Krasnoyarsk Reg Hosp 1, Krasnoyarsk 660022, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryavtsev, A.N.; Krasitskaya, V.V.; Petunin, A.I.; Burakov, A.Y.; Frank, L.A.

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Binding the immunoglobulins of human serum by nanodiamonds [Text] / A. V. Baron [et al.] // Dokl. Biochem. Biophys. - 2014. - Vol. 457, Is. 1. - P158-159, DOI 10.1134/S1607672914040127. - Cited References: 8. - This study was supported by the Presidium of the Russian Academy of Sciences (program no. 24, project no. 57). . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics

WOS
Держатели документа:
[Baron, A. V.
Puzyr, A. P.
Bondar, V. S.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
[Baron, A. V.
Osipov, N. V.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Osipov, N. V.
Olkhovskiy, I. A.] Minist Hlth & Social Dev Russian Federat, Hematol Res Ctr, Krasnoyarsk, Russia
[Olkhovskiy, I. A.] Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia
ИБФ СО РАН
КНЦ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
Baron, A.V.; Osipov, N.V.; Olkhovskiy, I.A.; Puzyr, A.P.; Bondar, V.S.; Presidium of the Russian Academy of Sciences [24, 57]

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Oxidative stress monitoring in biological samples by bioluminescent method / N. N. Remmel, N. M. Titova, V. A. Kratasyuk // Bulletin of Experimental Biology and Medicine. - 2003. - Vol. 136, Is. 2. - P. 209-211, DOI 10.1023/A:1026347830283 . - ISSN 0007-4888
Кл.слова (ненормированные):
Bioluminescent analysis -- Lipid peroxidation -- Malonic dialdehyde -- Oxidative stress -- malonaldehyde -- animal tissue -- article -- bioluminescence -- brain tissue -- controlled study -- fluorescence -- freeze drying -- kidney parenchyma -- lipid peroxidation -- liver -- male -- monitoring -- nonhuman -- oxidative stress -- Photobacterium phosphoreum -- rat -- serum -- Animals -- Bacteria -- Biological Assay -- Lipid Peroxidation -- Luminescent Measurements -- Malondialdehyde -- Oxidative Stress -- Photobacterium -- Rats -- Tissue Extracts -- Animalia -- Negibacteria -- Photobacterium -- Photobacterium phosphoreum
Аннотация: The integral bioluminescent biotest with lyophilized fluorescent bacteria was used for monitoring of LPO processes in tissue extracts and serum of rats exposed to stress. A relationship between the content of MDA (LPO indicator) and fluorescence of bacteria was observed in all biological samples.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Remmel, N.N.; Titova, N.M.; Kratasyuk, V.A.

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THE ENTRY INTO S-PERIOD OF NUCLEI IN HETERODIKARYONS MODIFIED BY THE CYCLOHEXIMIDE [Текст] / N. A. SETKOV, V. N. KAZAKOV, T. V. ANDREEVA // TSITOLOGIYA. - 1991. - Vol. 33, Is. 12. - P. 73-78. - Cited References: 16 . - ISSN 0041-3771

РУБ Cell Biology
Рубрики:
NIH 3T3 CELLS
   DNA-SYNTHESIS
   RESTING CELLS
   C-MYC
   FUSION
   FIBROBLASTS
   EXPRESSION
   GENES
Аннотация: Serum-deprived (0.2%) resting NIH 3T3 mouse fibroblasts were fused with serum-stimulated (10%) proliferating cells to elucidate mechanisms of entering into S-period operating in the nuclei of the heterokaryons under the effect of cycloheximide - an inhibitor of protein synthesis. Using radioautography DNA synthesis was investigated in mono-, homo- and heterodikaryons. After short (0.5-3.0 h) depressing of protein synthesis, the nuclei of stimulated cells in heterokaryons were found to enter into S-period. Under these conditions no induction of DNA synthesis was found in the nuclei of resting cells in heterodikaryons. In other experiments, resting cells were under the effect of cycloheximide during 2-4 h before the fusion, that led to a great induction of DNA synthesis in the nuclei of these cells in heterodikaryons. The data obtained are consistent with the idea of fibroblast transition to the rest under the action of labile proteins-repressors.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; KAZAKOV, V.N.; ANDREEVA, T.V.

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HEPATOCYTES IN HETEROKARYONS DO NOT RETARD THE ENTRY OF STIMULATED FIBROBLAST NUCLEI INTO THE S-PERIOD [Текст] / N. A. SETKOV, V. N. KAZAKOV, T. V. ANDREYEVA // TSITOLOGIYA. - 1991. - Vol. 33, Is. 6. - P. 76-85. - Cited References: 39 . - ISSN 0041-3771

РУБ Cell Biology
Рубрики:
HUMAN-DIPLOID FIBROBLASTS
   LIVER PLASMA-MEMBRANES
   DNA-SYNTHESIS
   PRIMARY CULTURE
   MOUSE HEPATOCYTES
   CELLS INVITRO
   SENESCENT
   GROWTH
   INHIBIT
   PHASE
Аннотация: Serum-deprived (0.2 %) resting and serum-stimulated (10 %) proliferating NIH 3T3 mouse fibroblasts were fused with hepatocytes from intact, regenerating and embryonic mouse livers to elucidate mechanisms of liver cell proliferation, DNA synthesis being investigated in nuclei of heterokaryons and non-fused cells using radioautography. Hepatocytes in heterokaryons were found to have no inhibitory effect on the entry of stimulated fibroblast nuclei into the S-period, but on the contrary they were involved in DNA synthesis. In addition, the nuclei in heterokaryons mutually stimulated each other to enter the S-period. In their turn, the resting fibroblasts did not prevent the proliferating hepatocytes from the regenerating and embryonic livers to enter the S-period. Possible reasons of the absence of inhibitory effect of differentiated cells in heterokaryons are discussed. The data obtained enable us to conclude that the mechanism of proliferative process control in resting immortalized cells differs from that in differentiated cells where proliferation seems to be stopped without affecting the endogenous inhibitor postulated for the resting and ageing fibroblasts.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; KAZAKOV, V.N.; ANDREYEVA, T.V.

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PROTEIN-SYNTHESIS INHIBITORS, LIKE GROWTH-FACTORS, MAY RENDER RESTING 3T3 CELLS COMPETENT FOR DNA-SYNTHESIS - A AUTORADIOGRAPHIC AND CELL-FUSION STUDY [Text] / N. A. SETKOV [et al.] // Cell Prolif. - 1992. - Vol. 25, Is. 3. - P. 181-191, DOI 10.1111/j.1365-2184.1992.tb01393.x. - Cited References: 20 . - ISSN 0960-7722

РУБ Cell Biology
Рубрики:
C-MYC
   CYCLOHEXIMIDE
   FIBROBLASTS
   EXPRESSION
   INDUCTION
   GENES
   FOS
Аннотация: Serum-deprived (0.1-0.2%) resting NIH 3T3 mouse fibroblasts pre-incubated with cycloheximide (7.5-mu-g/ml), or puromycin (10-mu-g/ml), were fused with stimulated cells taken 10 h after changing the medium to one containing 10% serum, and DNA synthesis was investigated in the nuclei of monokaryons, homodikaryons and heterodikaryons using radioautography with the double-labelling technique. Pre-incubation of resting cells with inhibitors of protein synthesis for 1-4 h abolished their ability to suppress DNA synthesis in stimulated nuclei in heterokaryons. Three hours after the removal of cycloheximide from the medium, the resting cells acquired once again the inhibitory capacity for entry of stimulated nuclei into the S period. This inhibitory influence disappeared also in the case of post-fusion cycloheximide application as well as following an 8-12 h pre-treatment of resting cells with actinomycin D (1-mu-g/ml) prior to fusion. Pre-incubation of resting cells for 12 h with PDGF (1 u/ml-1) followed by an 8-48 h incubation in serum-free medium stimulated the onset of DNA synthesis. A brief exposure (45 min) of resting cells to cycloheximide (7.5-mu-g/ml), or puromycin (7.5-mu-g/ml), exerted a similar effect, inducing by itself the entry of cells into the S period. The results support the assumption that acquirement, by resting cells, of competence for DNA replication includes as a necessary step the down-regulation of intracellular growth inhibitors whose formation depends on protein synthesis.

WOS
Держатели документа:
ACAD SCI USSR,INST BIOPHYS,KRASNOYARSK,USSR
WA ENGELHARDT MOLEC BIOL INST,MOSCOW,USSR
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; KAZAKOV, V.N.; ROSENWALD, I.B.; MAKAROVA, G.F.; EPIFANOVA, O.I.

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10.

Brief exposures of resting fibroblasts to okadaic acid stimulate DNA synthesis [Text] / N. A. Setkov, O. I. Epifanova // Cell Prolif. - 1997. - Vol. 30, Is. 1. - P. 7-19, DOI 10.1111/j.1365-2184.1997.tb00912.x. - Cited References: 32 . - ISSN 0960-7722

РУБ Cell Biology
Рубрики:
PDGF-INDUCED PROLIFERATION
   HAMSTER-EMBRYO CELLS
   PROTEIN-PHOSPHORYLATION
   PHOSPHATASE INHIBITORS
   MOUSE FIBROBLASTS
   3T3 CELLS
   FUSION
   TRANSFORMATION
   INDUCTION
   ARREST
Аннотация: To study further the factors providing for cellular quiescence, we used okadaic acid (OA) at concentrations (0.1, 1, 10 or 100 nM) inhibiting type 1 and/or type 2A protein phosphatases in mammalian cell cultures. Brief (2 h) exposure of resting (0.2% serum for 72 h) NIH 3T3 mouse fibroblasts to OA with subsequent incubation of cells in a medium with 0.2% serum, stimulated DNA synthesis at all concentrations studied. Maximal stimulation was observed following pre-incubation of resting cells with 10 nM OA. Treatment of cycling cells (10% serum) with OA (2 h pulses at 12 h intervals for 72 h) prevented their exit to the resting state on transfer to a medium with 0.2% serum. Brief exposures of resting cells to OA did not affect the rate of protein synthesis. OA pulses in the late pre-replicative period had no effect on the entry of serum-stimulated cells into the S phase. Cell fusion experiments with resting (serum-deprived) and proliferating (serum-stimulated) NIH 3T3 cells, using radioautography with a double-labelling technique, revealed that pre-incubation of resting cells with OA for 2 h before and after fusion abrogates their ability to suppress the onset of DNA synthesis in the nuclei of proliferating cells in heterodikaryons. The results indicate that protein phosphatases of type 1 and/or 2A may be involved in the growth-arrest machinery that provides for cellular quiescence.

WOS
Держатели документа:
VA ENGELHARDT MOL BIOL INST,MOSCOW 117984,RUSSIA
RUSSIAN ACAD SCI,INST BIOPHYS,SIBERIAN BRANCH,KRASNOYARSK,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.; Epifanova, O.I.

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11.

Oxidative stress monitoring in biological samples by bioluminescent method [Text] / N. N. Remmel', N. M. Titova, V. A. Kratasyuk // Bull. Exp. Biol. Med. - 2003. - Vol. 136, Is. 2. - P. 209-211, DOI 10.1023/A:1026347830283. - Cited References: 11 . - ISSN 0007-4888

РУБ Medicine, Research & Experimental

Кл.слова (ненормированные):
bioluminescent analysis -- oxidative stress -- lipid peroxidation -- malonic dialdehyde
Аннотация: The integral bioluminescent biotest with lyophilized fluorescent bacteria was used for monitoring of LPO processes in tissue extracts and serum of rats exposed to stress. A relationship between the content of MDA (LPO indicator) and fluorescence of bacteria was observed in all biological samples.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk, Russia
Krasnoyarsk State Univ, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Remmel', N.N.; Titova, N.M.; Kratasyuk, V.A.

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12.

Inhibitors of protein biosynthesis can stimulate proliferation of mouse hepatocytes in vitro [Text] / N. A. Setkov, A. V. Eremeev // Biol. Bull. - 2003. - Vol. 30, Is. 3. - P. 212-219, DOI 10.1023/A:1023843409416. - Cited References: 43 . - ISSN 1062-3590

РУБ Biology
Рубрики:
TRANSFORMING-GROWTH-FACTOR
   RAT-LIVER REGENERATION
   FACTOR-BETA
   DNA-SYNTHESIS
   PRIMARY CULTURE
   PARTIAL-HEPATECTOMY
   EPITHELIAL-CELLS
   EXPRESSION
   MECHANISMS
   MODULATION
Аннотация: Hepatocyte proliferation in the liver regenerating after partial hepatectomy ceases when the organ is restored, and the mechanism of this phenomenon is still unclear. In the experiments on fusing hepatocytes from the reoenerated mouse liver (15 days after partial hepatectomy) with NIH 3T3 mouse fibroblasts, we revealed no DNA synthesis in the nuclei of stimulated fibroblasts in heterokaryons (in the presence of hepatocyte nuclei), whereas DNA synthesis in nonfused cells was undisturbed. In this work, our purpose was to find out whether the suppression of DNA synthesis in heterokaryons could be due to the appearance in hepatocytes of some endogenous factors having an inhibitory effect on proliferation. To this end, hepatocytes from the mouse liver regenerated after partial hepatectomy were treated with cycloheximide for 1-4 h and were then fused with stimulated fibroblasts. Such a short-term treatment of hepatocytes with cycloheximide proved to result in the loss of their ability to inhibit DNA synthesis in the nuclei of stimulated or quiescent fibroblasts in heterokaryons, but hepatocytes proper actively proliferated in the medium with a low serum content (0.2%). When the mice with the liver reoenerated after partial hepatectomy were treated with a single sublethal dose of cycloheximide (3 mg/kg), their hepatocytes taken two days after this treatment had no inhibitory effect. Puromycin, another inhibitor of protein synthesis, had the same effect on hepatocytes. These results may be interpreted as evidence that the final stage of liver regeneration after damage is controlled by the factors having a C C negative effect on cell proliferation.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.; Eremeev, A.V.

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13.

Impact of enzyme stabilizers on the characteristics of biomodules for bioluminescent biosensors / V. Lonshakova-Mukina, E. Esimbekova, V. Kratasyuk // Sens Actuators, B Chem. - 2015. - Vol. 213. - P244-247, DOI 10.1016/j.snb.2015.02.061 . - ISSN 0925-4005
Кл.слова (ненормированные):
Bioluminescence -- Body fluids -- Carrier concentration -- Enzymes -- Starch -- Bioluminescent biosensor -- Bovine serum albumins -- Dithiothreitol -- Maximum permissible concentration -- Mercaptoethanol -- Oxidoreductases -- Storage stability -- Toxic substances -- Biosensors
Аннотация: The biomodule of bioluminescent biosensor based on a coupled enzyme system NADH:FMN-oxidoreductase and luciferase, co-immobilized with substrates in dried starch or gelatin gels, has been developed. We studied the impact of several stabilizers - dithiothreitol (DTT), bovine serum albumin (BSA) and mercaptoethanol (ME) on the biomodule's activity, storage stability and sensitivity to toxic substances. The inclusion of stabilizers increases the activity of the biological module by more than 150%. To achieve the combination of high activity, prolonged storage time and acute sensitivity to toxic substances within maximum permissible concentration we used starch gel as a carrier adding 100 ?M DTT to the immobilized preparation. The gelatin-based biological module had greater storage stability than the starch-based one but demonstrated less sensitivity to toxic substances. © 2015 Elsevier B.V. All rights reserved.

Scopus,
WOS
Держатели документа:
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, pr. Svobodnyi 79Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Akademgorodok 50/50Krasnoyarsk, Russian Federation
ИБФ СО РАН

Доп.точки доступа:
Lonshakova-Mukina, V.; Esimbekova, E.; Kratasyuk, V.

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14.

Spectral Changes of Erythrosin B Luminescence Upon Binding to Bovine Serum Albumin [Text] / N. V. Sablin, M. A. Gerasimova, E. V. Nemtseva // Russ. Phys. J. - 2016. - Vol. 58, Is. 12. - P1797-1803, DOI 10.1007/s11182-016-0719-6. - Cited References:16. - This work was supported in part by the Russian Academy of Sciences (The program "Molecular and Cell Biology", project No. 6.8), the Ministry of Education and Science of the Russian Federation (project No. 1762), and the Federal Agency of scientific organizations of the Russian Federation (project No. VI 57.1.1). . - ISSN 1064-8887. - ISSN 1573-9228

РУБ Physics, Multidisciplinary
Рубрики:
ROOM-TEMPERATURE
   AQUEOUS-SOLUTION
   PHOSPHORESCENCE
   FLUORESCENCE
   EOSIN
Кл.слова (ненормированные):
erythrosin B -- phosphorescence -- delayed fluorescence -- quantum yield -- phosphorescence lifetime -- bovine serum albumin
Аннотация: Changes in absorption, fluorescence, phosphorescence, and delayed fluorescence spectra of erythrosin B are studied in the presence of bovine serum albumin at room temperature. Spectral and chronoscopic characteristics of the observed photophysical processes are defined. The binding of erythrosin B with the protein followed by spectral changes is demonstrated. Absorption and fluorescence spectra of the dye in the bound state are described, the binding mechanism is analyzed. The binding parameters of the dye-protein complex are estimated.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.

Доп.точки доступа:
Sablin, N. V.; Gerasimova, M. A.; Nemtseva, E. V.; Russian Academy of Sciences [6.8]; Ministry of Education and Science of the Russian Federation [1762]; Federal Agency of scientific organizations of the Russian Federation [VI 57.1.1]

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15.

Similarity of decay-associated spectra for tryptophan fluorescence of proteins with different structures / E. V. Nemtseva, O. O. Lashchuk, M. A. Gerasimova // Biophysics. - 2016. - Vol. 61, Is. 2. - P193-199, DOI 10.1134/S0006350916020111 . - ISSN 0006-3509
Кл.слова (ненормированные):
denaturation -- dielectric relaxation -- fluorescence lifetime -- tertiary protein structure -- tryptophan
Аннотация: Tryptophan fluorescence lifetimes were analyzed for three proteins: human serum albumin, bovine serum albumin, and bacterial luciferase, which contain one, two, and seven tryptophan residues, respectively. For all of the proteins, the fluorescence decays were fitted by three lifetimes: ?1 = 6–7 ns, ?2 = 2.0–2.3 ns, and ?3 ? 0.1 ns (the native state), and ?1 = 4.4–4.6 ns, ?2 = 1.7–1.8 ns, and ?3 ? 0.1 ns (the denatured state). Corresponding decay-associated spectra had similar peak wavelengths and spectrum half-widths both in the native state (??1max = 342 nm, ??2max = 328 nm, and ??3max = 315 nm), and in the denatured state (??1max = 350 nm, ??2max= 343 nm, and ??3max= 317 nm). The differences in the steady-state spectra of the studied proteins were accounted for the individual ratio of the lifetime component contributions. The lifetime components were compared with a classification of tryptophan residues in the structure of these proteins within the discrete states model. © 2016, Pleiades Publishing, Inc.

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Держатели документа:
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Nemtseva, E. V.; Lashchuk, O. O.; Gerasimova, M. A.

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16.

Battle of GLP-1 delivery technologies / M. Yu [et al.] // Adv. Drug Deliv. Rev. - 2018, DOI 10.1016/j.addr.2018.07.009 . - ISSN 0169-409X
Кл.слова (ненормированные):
Albumin fusion -- Exenatide -- Fatty acid conjugate -- Fc fusion -- GLP-1 receptor agonist -- Half-life -- Peptide delivery -- Pharmacokinetics
Аннотация: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) belong to an important therapeutic class for treatment of type 2 diabetes. Six GLP-1 RAs, each utilizing a unique drug delivery strategy, are now approved by the Food and Drug Administration (FDA) and additional, novel GLP-1 RAs are still under development, making for a crowded marketplace and fierce competition among the manufacturers of these products. As rapid elimination is a major challenge for clinical application of GLP-1 RAs, various half-life extension strategies have been successfully employed including sequential modification, attachment of fatty-acid to peptide, fusion with human serum albumin, fusion with the fragment crystallizable (Fc) region of a monoclonal antibody, sustained drug delivery systems, and PEGylation. In this review, we discuss the scientific rationale of the various half-life extension strategies used for GLP-1 RA development. By analyzing and comparing different approved GLP-1 RAs and those in development, we focus on assessing how half-life extending strategies impact the pharmacokinetics, pharmacodynamics, safety, patient usability and ultimately, the commercial success of GLP-1 RA products. We also anticipate future GLP-1 RA development trends. Since similar drug delivery strategies are also applied for developing other therapeutic peptides, we expect this case study of GLP-1 RAs will provide generalizable concepts for the rational design of therapeutic peptides products with extended duration of action. © 2018 Elsevier B.V.

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Держатели документа:
Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, 428 Church St, Ann Arbor, MI, United States
Amneal Pharmaceuticals, 50 Horseblock Rd, Brookhaven, NY, United States
Siberian Federal University, 79 Svobodnuy Ave, Krasnoyarsk, Russian Federation
Institute of Biophysics SBRAS, 50 Akademgorodok, Russian Federation
Biointerfaces Institute, NCRC, 2800 Plymouth Rd, Ann Arbor, MI, United States
Department of Biomedical Engineering, 2200 Bonisteel Blvd, Ann Arbor, MI, United States

Доп.точки доступа:
Yu, M.; Benjamin, M. M.; Srinivasan, S.; Morin, E. E.; Shishatskaya, E. I.; Schwendeman, S. P.; Schwendeman, A.

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17.

Bioluminescent aptamer-based sandwich-type assay of anti-myelin basic protein autoantibodies associated with multiple sclerosis / V. V. Krasitskaya [et al.] // Anal. Chim. Acta. - 2019. - Vol. 1064. - P112-118, DOI 10.1016/j.aca.2019.03.015. - Cited References:29. - This work was supported by the Russian Foundation for Basic Research (RFBR), Russia, under the grant No 17-315-50027; Russian State funded budget projects No. AAAA-A17-117013050026-4 and AAAA-A17-117020210021-7. . - ISSN 0003-2670. - ISSN 1873-4324

РУБ Chemistry, Analytical
Рубрики:
ANTIBODIES
BIOMARKERS
RNA
Кл.слова (ненормированные):
Bioluminescent microassay -- RNA aptamers -- Autoantibodies to myelin basic -- protein -- Multiple sclerosis
Аннотация: Bioluminescent solid-phase sandwich-type microassay was developed to detect multiple sclerosis (MS)-associated autoantibodies in human sera. The assay is based on two different 2'-F-Py RNA aptamers against the target autoantibodies as biospecific elements, and Ca2+-regulated photoprotein obelin as a reporter. The paper describes elaboration of the assay and its application to 91 serum samples from patients with clinically definite MS and 86 ones from individuals healthy in terms of MS. Based on the receiver-operator curve (ROC) analysis, the chosen threshold value as clinical decision limit offers sensitivity of 63.7% and specificity of 94.2%. The area under the ROC curve (AUC) value of 0.87 shows a good difference between the groups under investigation. The likelihood ratio of 10.97 proves the diagnostic value of the assay and its potential as one of the laboratory MS-tests. (C) 2019 Elsevier B.V. All rights reserved.

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Держатели документа:
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys SB, Krasnoyarsk 660036, Russia.
RAS, Inst Chem Biol & Fundamental Med SB, Novosibirsk 630090, Russia.
State Med Univ, Krasnoyarsk 660022, Russia.

Доп.точки доступа:
Krasitskaya, Vasilisa V.; Chaukina, Valentina V.; Abroskina, Maria V.; Vorobyeva, Maria A.; Ilminskaya, Aleksandra A.; Kabilov, Marsel R.; Prokopenko, Semyon V.; Nevinsky, Georgy A.; Venyaminova, Alya G.; Frank, Ludmila A.; Russian Foundation for Basic Research (RFBR), Russia [17-315-50027]; Russian State [AAAA-A17-117013050026-4, AAAA-A17-117020210021-7]

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18.

Role of Hsp90 and ATP in modulating apyrase activity and firefly luciferase kinetics / M. A. Kirillova [et al.] // Int. J. Biol. Macromol. - 2019. - Vol. 131. - P691-696, DOI 10.1016/j.ijbiomac.2019.03.110 . - ISSN 0141-8130
Кл.слова (ненормированные):
Bioluminescence -- Heat shock protein 90 -- High-throughput screening -- adenosine triphosphate -- apyrase -- bovine serum albumin -- firefly luciferase -- heat shock protein 90 -- stabilizing agent -- Article -- bioluminescence -- clinical study -- conformation -- controlled study -- denaturation -- enzyme activity -- enzyme kinetics -- high throughput screening -- incubation time -- nonhuman -- protein protein interaction -- protein refolding -- temperature -- thermal denaturation -- time
Аннотация: The present manuscript describes a novel bioassay consisting of apyrase and heat shock protein 90 (Hsp90) without additional co-chaperone supplementation; intended for high-throughput screening of anti-cancer drugs and prognosis of stress. In this regard, Hsp90 and adenosine 5?-triphosphate (ATP) mediated firefly luciferase (FLuc) kinetics was investigated using apyrase and FLuc as client proteins. Bioluminescent assay containing Hsp90, ATP, and apyrase led to complete loss of luminescence at 50 °C which indicates the protective role of Hsp90 against thermal denaturation. Similarly, the assay sample comprising Hsp90, ATP, and FLuc showed 2 fold increments in luminescence than their counterparts. Introduction of bovine serum albumin (BSA) to the pre-incubated assay mixture led to an initial rise in the luminescence (28%) in comparison to the sample containing Hsp90, ATP and FLuc. Therefore, FLuc based HTS assays are not suitable for clinical samples which may contain stabilizing agents. However, thermally denatured FLuc and apyrase could not regain their active conformation even when Hsp90 and ATP were introduced in the assay system. This observation justifies the role of Hsp90 to be protective rather than a reparation agent when acts without co-chaperones. © 2019

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Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Kirillova, M. A.; Ranjan, R.; Esimbekova, E. N.; Kratasyuk, V. A.

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19.

Dietary buckwheat enhances sirtuin1 without calorie restriction / S. Pande, R. Ranjan, A. N. Shuvaev [et al.] // J. Cereal Sci. - 2020. - Vol. 94. - Ст. 103004, DOI 10.1016/j.jcs.2020.103004 . - ISSN 0733-5210
Кл.слова (ненормированные):
Calorie restriction -- Dietary buckwheat -- Male wistar rats -- SIRT1 expression
Аннотация: In the present investigation, the role of dietary intervention in male Wistar rats (n = 8, 3 groups) was studied to observe absolute sirtuin 1 (SIRT1) levels (expressed as ng mg?1 total protein) in serum, stomach, liver, and kidney. Dietary buckwheat at 30% (w/w) level of incorporation in the standard diet (Buckwheat Enriched Diet, BED) improved SIRT1 with values 0.933 ± 0.05, 210 ± 7, 63.26 ± 4, and 69.89 ± 3 in serum, stomach, liver, and kidney respectively when compared to the respective control values of 0.536 ± 0.03, 156 ± 23.3, 31.07 ± 2 and 47.11 ± 4. Moreover, BED though isocaloric to CR diet, led to weight gain (g) by 63.11 ± 3.8, ca. 10%, and 40% higher than control (56.27 ± 5.6) and CR (45.05 ± 4.1) diet groups. A marked rise in Feed Efficiency Ratio (FER) by ca. 37% while a 30% decrease in Feed Conversion Ratio (FCR) was observed for the BED group which supports unexpected weight gain in rats post-dietary intervention. The results justify the superior nutritional profile of buckwheat laden with essential nutrients, essential proteins, and bioactives. In contrast, Calorie Restriction (CR) resulted in a decline of the total protein content in circulation by 19%, while reduction of total protein in stomach, liver, and kidney was estimated to be 95%, 35.2%, and 27% respectively though SIRT1 values were comparatively the highest in all the samples studied. A 30-fold enhancement of SIRT1 in stomach post CR is presumed to counter enhanced stress in gastric tissues. Therefore, mild to moderate expression of SIRT1 may confer beneficial effects such as delayed aging and stress resistance but exceedingly high SIRT1 may evoke increased oxidative stress. © 2020 Elsevier Ltd

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Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodny Prospect 79, Krasnoyarsk, 660041, Russian Federation
Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University Named After Prof. V.F. Voino-Yasenetsky, P. Zheleznyaka 1, Krasnoyarsk, 660022, Russian Federation
Department of Biochemistry, Medical, Pharmaceutical & Toxicological Chemistry, Krasnoyarsk State Medical University Named After Prof. V.F. Voino-Yasenetsky, P. Zheleznyaka 1, Krasnoyarsk, 660022, Russian Federation
Scientific Research Institute of Medical Problems of the North, P. Zheleznyaka 3g, Krasnoyarsk, 660022, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Akademgorodok 50/50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Pande, S.; Ranjan, R.; Shuvaev, A. N.; Malinovskaya, N. A.; Ryazanova, M.; Salmina, A. B.; Kolenchukova, O. A.; Kratasyuk, V. A.

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20.

Highly-sensitive graphene field effect transistor biosensor using PNA and DNA probes for RNA detection / M. Tian, M. Qiao, C. Shen [et al.] // Appl Surf Sci. - 2020. - Vol. 527. - Ст. 146839, DOI 10.1016/j.apsusc.2020.146839 . - ISSN 0169-4332
Кл.слова (ненормированные):
Graphene field effect transistor -- PNA probe -- RNA detection -- Biosensors -- Clinical research -- Diagnosis -- DNA -- Field effect transistors -- Graphene -- RNA -- Biomedical research -- Clinical diagnosis -- Electrical response -- Graphene field-effect transistors -- Limit of detection -- Quantitative detection -- Three orders of magnitude -- Ultrasensitive detection -- Graphene transistors
Аннотация: DNA probe-based biosensors have been widely developed for detecting a range of analytes. However, the DNA probe-based sensors suffer from many problems, such as long hybridization time, background electrical noise, and relatively poor specificity. In this paper, we report the ultrasensitive detection for RNA by graphene field effect transistor (G-FET) biosensor using PNA and DNA probes. The limit of detection (LOD) of the PNA probe-modified G-FET sensor is down to 0.1 aM, which is three orders of magnitude lower than that of DNA probe-modified G-FET sensor. We demonstrate that both PNA and DNA probe-modified G-FET have great potential in quantitative detection of RNA. A good linear electrical response to RNA concentrations is obtained in a broad range from 0.1 aM to 1 pM for PNA probe-modified G-FET and from 100 aM to 1 pM for DNA probe-modified G-FET, respectively. The PNA probe-modified G-FET sensors significantly reduce the detection time compared to DNA probe-modified G-FET sensors. Moreover, the electrical response of PNA probe-modified G-FET biosensor to non-complementary RNA is negligible, showing high specificity for RNA detection. What's more, the G-FET sensor was also used to detect RNA in human serum, making it a promising way for future detection of RNA in biomedical research and early clinical diagnosis. © 2020 Elsevier B.V.

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Держатели документа:
Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Tian, M.; Qiao, M.; Shen, C.; Meng, F.; Frank, L. A.; Krasitskaya, V. V.; Wang, T.; Zhang, X.; Song, R.; Li, Y.; Liu, J.; Xu, S.; Wang, J.

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