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Population heterogeneity of plasmid-bearing and plasmid-free Bacillus subtilis strains under different environmental conditions / T. Yu. Krylova [et al.] // Microbiology. - 2000. - Vol. 69, Is. 2. - P220-224 . - ISSN 0026-2617
Кл.слова (ненормированные):
Bacillus subtilis -- Heterogeneity -- Introduction -- Microcosm -- Recombinant plasmid
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (KmI)Inf+) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1?M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krylova, T.Yu.; Popova, L.Yu.; Pechurkin, N.S.; Kashperova, T.A.; Belyavskaya, V.A.

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Population heterogeneity of plasmid-bearing and plasmid-free bacillus subtilis strains under different environmental conditions / T. Yu. Krylova [и др.] // Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 270-275 . - ISSN 0026-3656
Кл.слова (ненормированные):
Bacillus subtilis -- Heterogeneity -- Introduction -- Microcosm -- Recombinant plasmid -- article -- Bacillus subtilis -- culture medium -- genetic recombination -- genetics -- plasmid -- Bacillus subtilis -- Culture Media -- Plasmids -- Recombination, Genetic
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (Km rInf +) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1 ? M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krylova, T.Yu.; Popova, L.Yu.; Pechurkin, N.S.; Kashperova, T.A.; Belyavskaya, V.A.

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A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2 [Text] / L. Y. Popova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 6. - P. 637-641. - Cited References: 13 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
Кл.слова (ненормированные):
microecosystem -- Bacillus subtilis -- recombinant plasmid -- interferon -- adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.

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Держатели документа:
Russian Acad Sci, Akademgorodok, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
State Sci Ctr VB Vektor, Sci Res Design & Technol Inst Biol Act Subst, Berdsk 633190, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Y.; Kargatova, T.V.; Maksimova, E.E.; Belyavskaya, V.A.

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Self-restoration of biocomponents as a mean to enhance biological life support systems reliability [Text] / S. I. Bartsev, V. A. Okhonin ; ed.: F Brechignac, , F Brechign // LIFE SCIENCES: ARTIFICIAL ECOSYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON PRESS LTD, 1999. - Vol. 24: F4 3 and F4 4 Symposia of COSPAR Scientific Commission F Held at 31st COSPAR Scientific Assembly (JUL 14-21, 1996, BIRMINGHAM, ENGLAND), Is. 3. - P. 393-396, DOI 10.1016/S0273-1177(99)00489-5. - Cited References: 5 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: One of the key problems of long-term space missions is limited service life of units. The only exceptions are biological components of biological Life Support Systems - higher plants or microorganisms. These components are capable of self-restoration: after complete disintegration, they can appear again from seeds or spores. The estimate of failure intensity of BLSS regeneration component includes: a number of self-sustained sections of the regeneration component; permissible boost (how many times can productivity of a component be increased); time required to repair (restore) a component; the crew existence time, when all LSS regeneration components fail; failure rate of one section of a regeneration component. Evaluations show that for hydrogen-oxidizing bacteria and micro-algae very high reliability is achieved even for one or two sections. In the case of higher plants (due to low rate of self-restoration) bio-regenerative module has to be divided into 10 self-sustained sections operating simultaneously. These measures can decrease the probability of catastrophe by a factor of 10 (C) 1999 COSPAR. Published by Elsevier Science Ltd.

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Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bartsev, S.I.; Okhonin, V.A.; Brechignac, F \ed.\; Brechign, , F \ed.\

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The architectonics of colonies of Bacillus subtilis 2335 [Text] / A. P. Puzyr' [et al.] // Microbiology. - 2002. - Vol. 71, Is. 1. - P. 57-64, DOI 10.1023/A:1017950316748. - Cited References: 12 . - ISSN 0026-2617

РУБ Microbiology

Кл.слова (ненормированные):
electron microscopy -- Bacillus subtills -- spores -- vegetative cells -- glycocalyx -- architectonics of bacterial colonies
Аннотация: Colonies grown from vegetative Bacillus subtilis 2335 cells had a standard structure, with bacillar cells occupying the whole colony volume. At the same time, the colonies of this bacterium grown from germinated spores had an abnormal structure characterized by the location of cells in a surface layer 100-200 mum thick at the colony boundary with the air. The glycocalyx of the colonies grown from spores was characterized by a wetting angle theta(e) of 120degrees-160degrees, whereas that of the colonies grown from vegetative cells had an angle theta(c) as low as 5degrees-30degrees. It is suggested that spores and vegetative cells follow different strategies of substrate colonization and that the architectonics of bacterial colonies is determined by the physicochemical properties of the glycocalyx.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr', A.P.; Mogil'naya, O.A.; Krylova, T.Y.; Popova, L.Y.

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Development and morphological features of biofilms formed by transgenic and wild type strains of Bacillus subtilis [Text] / O. A. Mogilnaya, T. Y. Krylova, L. Y. Popova // Microbiol. Res. - 2003. - Vol. 158, Is. 4. - P. 327-335, DOI 10.1078/0944-5013-00212. - Cited References: 17 . - ISSN 0944-5013

РУБ Microbiology
Рубрики:
ANTIBIOTIC-RESISTANCE
MICROBIAL BIOFILMS
COMMUNITY
Кл.слова (ненормированные):
Bacillus subtilis -- biofilms -- transgenic microorganisms -- electron microscopy
Аннотация: The study addressed the ability of the transgenic strain (TM) B. subtilis 2335/pBMB 105 (Km(r)Inf(+)) to form biofilms on the surface of liquid media of various compositions, inoculated with vegetative cells and spores. The morphological features of these biofilms do not differ from those of the films formed by the recipient strain (WT) B. subtilis 2335 (Km(s)). However, the TM and the natural one differ in the dynamics of biofilm formation and the cellular composition of the films. Biofilms of the TM are formed earlier, develop at a higher rate, but decompose later than the films of the WT. When the medium is inoculated with vegetative cells, sporulation in the biofilms of both strains undergoes glucose repression; no such effect is observed when the medium is inoculated with spores. The TM does not form films when the medium is inoculated with spores and supplemented with glycerin and kanamycin.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mogilnaya, O.A.; Krylova, T.Y.; Popova, L.Y.

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High temperature effect on microflora of radish root-inhabited zone and nutrient solutions for radish growth [Text] / E. V. Borodina, L. S. Tirranen ; ed.: G Horneck, ME Vazquez, Vazquez, ME // SPACE LIFE SCIENCES: MISSIONS TO MARS, RADIATION BIOLOGY, AND PLANTS AS A FOUNDATION FOR LONG-TERM LIFE SUPPORT SYSTEMS IN SPACE. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: F0 1 and F1 3-F2 3 Symposia of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 1. - P. 235-240, DOI 10.1016/S0273-1177(02)00741-X. - Cited References: 7 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: The effect of high temperatures (35 and 45 degreesC) on microflora of the root zone of radish plants grown in phytotron was evaluated by the response of microorganisms from 9 indicator groups. Phytotron air temperature elevated to 35 degreesC for 20 hours caused no significant changes in qualitative and quantitative composition of the root microflora in experimental plants. By the end of the experiment, the species diversity of microflora had changed. The amount of phytopathogenic microorganisms decreased which can be interpreted as more stable co-existence of microflora with plants. The numbers of microbes from other indicator groups was in dynamic equilibrium. The plants' condition did not deteriorate either. Exposure to the temperature of 45 degreesC for 7 hours have been found to change the numbers and species diversity in the radish root zone microflora. The microorganisms were observed to increase their total numbers at the expense of certain indicator groups. Bacteria increased spore forms at the stage of spores. Colon bacillus bacteria of increased their numbers by the end,of experiment by an order. By the end of experiment the roots of experiment plants had microscopic fungi from. Mucor, Aspergillus, Trichoderma, Cladosporium genera. The observed changes in the microbial complex seem to be associated with the changes of root emissions and general deterioration of the plants' condition. It is suggested that the response of the microorganisms can be indicative of the condition of plants under investigation. (C) 2002 Published by Elsevier Science Ltd on behalf of COSPAR.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Borodina, E.V.; Tirranen, L.S.; Horneck, G \ed.\; Vazquez, ME \ed.\

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The use of glowing wood as a source of luminescent culture of fungus mycelium [Text] / A. P. Puzyr, S. E. Medvedeva, V. S. Bondar // Mycosphere. - 2016. - Vol. 7, Is. 1. - P1-17, DOI 10.5943/mycosphere/7/1/1. - Cited References:22. - The authors are grateful to Prof. A. Frank, Director of North Borneo Biostation, for the opportunity to carry out studies of glowing wood; to Nadezhda N. Kudashova, a senior researcher at the Institute of Biology and Biophysics at the Tomsk University, for identifying the species of nonluminous fungi. This study was supported by grant no. 11.G34.31.0058 (RF Government) and Projects no. 71 (SB RAS). . - ISSN 2077-7000

РУБ Mycology
Рубрики:
BIOLUMINESCENCE CHARACTERISTICS
NEONOTHOPANUS-NAMBI
LIGHT-EMISSION
Кл.слова (ненормированные):
Bioluminescence -- culture of luminous mycelia -- kinetics of luminescent -- reaction -- light emitting wood -- luminous fungus
Аннотация: In studies of fungal bioluminescence, not only fruiting bodies and spores of the fungus, but also samples of luminescent wood, leaf litter or soil may need to be used to derive pure mycelial culture. This study describes an approach to isolating the culture of luminescent fungal mycelium from samples of light-emitting wood found on Borneo Island in November-December 2013. A GelDoc XR Imaging System (Bio-Rad Laboratories, Inc., U.S.) was used for the first time to monitor luminescence and select luminous samples. This study shows that for successful isolation of the culture of luminescent mycelium out of the luminescent wood found in the forest, it is imperative to keep the samples moist (mycelium alive until there is water), while immediate and aseptic delivery of the samples to the laboratory is not a crucial condition (inner layers of wood is "sterile"). Investigation of the growth features of the isolated mycelium in various growing conditions revealed some peculiar properties of its luminescence in comparison with the known luminescent cultures of basidiomycetes. When grown on solid nutrient media, mycelium exhibits low growth rates, long-lasting luminescence (140 days or longer), and emergence and disappearance of local zones with high levels of light emission. Mycelium produced in submerged culture does not emit light, and this effect must be caused by the absence or a very low level of the luminescent reaction substrate in the biomass. The luminescence system isolated from mycelial biomass did not induce luminescent reaction in vitro upon the addition of NADPH (recording intensity is 60 100 URL/sec). We found that enzymes of the luminescence systems isolated from mycelium pellets retained their activity and catalyzed luminescent reaction when a hot extract of the luminous fungus Armillaria sp. (IBSO 2360) was added (near 1900 URL/sec). The same effect was obtained after addition of hot extracts from the fruiting bodies of nonluminous higher fungi Pholiota squarrosa, Cortinarius sp., Hypholoma capnoides and Chroogomphus rutilus (near 3500 URL/sec). The pure culture of luminescent mycelium has been registered in the Culture Collection of IBP SB RAS as IBSO 2371; now it can be used for various in vivo and in vitro studies, including identification of the fungus.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Puzyr, A. P.; Medvedeva, S. E.; Bondar, V. S.; RF Government [11.G34.31.0058]; SB RAS [71]

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Fungicidal activity of slow-release P(3HB)/TEB formulations in wheat plant communities infected by Fusarium moniliforme / T. Volova, S. Prudnikova, N. Zhila // Environ. Sci. Pollut. Res. - 2018. - Vol. 25, Is. 1. - P552-561, DOI 10.1007/s11356-017-0466-4. - Cited References:28. - The research was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project no AAA-A17-117013050028-8). . - ISSN 0944-1344. - ISSN 1614-7499

РУБ Environmental Sciences
Рубрики:
BIODEGRADABLE POLY-3-HYDROXYBUTYRATE
TEBUCONAZOLE
Кл.слова (ненормированные):
Tebuconazole -- Poly-3-hydroxybutyrate -- Fungicidal effect -- Fusariummoniliforme -- Wheat plant communities -- Root rot
Аннотация: Fungicidal activity of experimental tebuconazole (TEB) formulations was investigated in laboratory soil ecosystems in wheat plant communities infected by Fusarium moniliforme. TEB was embedded in the matrix of poly-3-hydroxybutyrate, shaped as films and microgranules. These formulations were buried in the soil with wheat plants, and their efficacy was compared with that of commercial formulation Raxil and with the effect of pre-sowing treatment of seeds. In the experiment with the initially infected seeds and a relatively low level of natural soil infection caused by Fusarium fungi, the effects of the experimental P(3HB)/TEB formulations and Raxil were comparable. However, when the level of soil infection was increased by adding F. moniliforme spores, P(3HB)/TEB granules and films reduced the total counts of fungi and the abundance of F. moniliforme more effectively than Raxil. Seed treatment or soil treatment with Raxil solution showed an increase in the percentage of rot-damaged roots in the later stages of the experiment. In the early stage (between days 10 and 20), the percentage of rot-damaged roots in the soil with TEB embedded in the slowly degraded P(3HB) matrix was similar to that in the soil with Raxil. However, the efficacy of P(3HB)/TEB formulations lasted longer, and in later stages (between days 20 and 30), the percentage of rot-damaged roots in that group did not grow. In experiments with different TEB formulations and, hence, different fungicidal activities, the increase in plant biomass was 15-17 to 40-60% higher than in the groups where TEB was applied by using conventional techniques.

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Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Inst Biophys SB,Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, Tatiana G.; Prudnikova, Svetlana V.; Zhila, Natalia O.; Russian Academy of Sciences [AAA-A17-117013050028-8]

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10.

Production and characterization of bioaerosols for model validation in spacecraft environment / A. Salmela [et al.] // J. Environ. Sci. - 2018. - Vol. 69. - P227-238, DOI 10.1016/j.jes.2017.10.016. - Cited References:28. - The research leading to these results has received funding from the European Union Seventh Framework Programme (FP/2007-2013) under grant agreement number 263076 within the BIOSMHARS Project (BIO contamination Specific Modeling in Habitats Related to Space). . - ISSN 1001-0742. - ISSN 1878-7320

РУБ Environmental Sciences
Рубрики:
INDOOR AIR
   MICROBIAL-CONTAMINATION
   BUILDING-MATERIALS
   FUNGAL
Кл.слова (ненормированные):
Bioaerosol -- Modeling -- CFD -- Spacecraft -- Fungi -- Bacteria
Аннотация: This study aimed to evaluate the suitability of two bioaerosol generation systems (dry and wet generation) for the aerosolization of microorganisms isolated from the International Space Station, and to calibrate the produced bioaerosols to fulfill the requirements of computational fluid dynamics model (CFD) validation. Concentration, stability, size distribution, agglomeration of generated bioaerosol and deposition of bioaerosols were analyzed. In addition, the dispersion of non-viable particles in the air was studied. Experiments proved that wet generation from microbial suspensions could be used for the production of well-calibrated and stabile bioaerosols for model validation. For the simulation of the natural release of fungal spores, a dry generation method should be used. This study showed that the used CFD model simulated the spread of non-viable particles fairly well. The mathematical deposition model by Lai and Nazaroff could be used to estimate the deposition velocities of bioaerosols on surfaces, although it somewhat underestimated the measured deposition velocities. (c) 2017 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.

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Держатели документа:
Univ Eastern Finland, Dept Environm & Biol Sci, POB 1627, FI-70211 Kuopio, Finland.
VTT Ltd, POB 1300, FI-33100 Tampere, Finland.
Belgian Nucl Res Ctr, Microbiol Unit, B-2400 Mol, Belgium.
Inst Med & Physiol Spatiales, BP 74404, F-31405 Toulouse, France.
Inst Biomed Problems, 76-A Khoroshev Skoye Shosse, RU-123007 Moscow, Russia.
RAS, Inst Biophys SB, RU-660036 Krasnoyarsk, Russia.

Доп.точки доступа:
Salmela, Anniina; Kokkonen, Eero; Kulmala, Ilpo; Veijalainen, Anna-Maria; van Houdt, Rob; Leys, Natalie; Berthier, Audrey; Viacheslav, Ilyin; Kharin, Sergey; Morozova, Julia; Tikhomirov, Alexander; Pasanen, Pertti; Van, Rob; European Union [263076]

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