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Detoxification of AM-241 solutions by humic substances: Bioluminescent monitoring / T. Rozhko [et al.] // Analytical and Bioanalytical Chemistry. - 2011. - Vol. 400, Is. 2. - P329-334, DOI 10.1007/s00216-010-4442-9 . - ISSN 1618-2642
Кл.слова (ненормированные):
Detoxification -- Humic substances -- Ionizing radiation -- Luminous bacteria -- Bacterial cells -- Bottom sediments -- Humic substances -- Luminescent intensity -- Luminous bacteria -- Natural transformations -- Organic substances -- Photobacterium phosphoreum -- Physiological activity -- Protecting agent -- Water solutions -- Anoxic sediments -- Bacteriology -- Bioluminescence -- Detoxification -- Ionizing radiation -- Luminance -- Radiation shielding -- Radioactivity -- Bacteria -- americium -- radioisotope -- article -- bioremediation -- chemistry -- environmental monitoring -- evaluation -- humic substance -- instrumentation -- luminescence -- metabolism -- methodology -- Photobacterium -- water pollutant -- Americium -- Biodegradation, Environmental -- Environmental Monitoring -- Humic Substances -- Luminescence -- Photobacterium -- Radioisotopes -- Water Pollutants, Chemical -- Bacteria (microorganisms) -- Photobacterium phosphoreum
Аннотация: The study addresses the effect of humic substances on marine luminous bacteria Photobacterium phosphoreum exposed to Am-241 (3,000 Bq L -1, water solution). Luminescent intensity of the bacteria was applied as a marker of their physiological activity. Humic substances have been found to reduce the effect of Am-241 on luminescence, decrease damage to cells, and change distribution of Am-241 between bacterial cells and intercellular media. It was shown that water-soluble humic substances, being products of natural transformation of organic substances in soil and bottom sediments, can serve as protecting agents for water microorganisms exposed to alpha radionuclides. В© 2010 Springer-Verlag.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk 660036, Russian Federation
Irkutsk State University, Irkutsk 664003, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rozhko, T.; Bondareva, L.; Mogilnaya, O.; Vydryakova, G.; Bolsunovsky, A.; Stom, D.; Kudryasheva, N.

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Understanding the mechanisms of blooming of phytoplankton in Lake Shira, a saline lake in Siberia (the Republic of Khakasia) / A. G. Degermendzhy, R. D. Gulati // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P331-340 . - ISSN 1386-2588
Кл.слова (ненормированные):
Carbon budget -- Cyanobacteria -- Heterotrophic bacteria -- Hydrogen sulphide -- Mathematical models of stratification -- Meromictic lakes -- Microbial loop -- Stratification -- Trophic scheme -- algal bloom -- ecosystem modeling -- limiting factor -- nutrient availability -- phytoplankton -- saline lake -- trophic interaction -- Russian Federation -- algae -- Bacteria (microorganisms) -- Cyanobacteria -- Lyngbya -- Lyngbya contorta
Аннотация: The paper summarises the results of a three-year research study (European Union Grant: INTAS 97-0519) aimed at investigating the planktonic populations and trophic organization of the Lake Shira ecosystem - a saline lake in Khakasia, Siberia. The lake exhibits a stable summer-autumn stratification of the chemical-biological components. The mechanisms responsible for the 'blooming' of phytoplankton in the deeper layers were investigated in greater detail, using data from both field and laboratory experiments. The spectra of nutrition were examined to estimate the relationships between the specific growth rates of the hydrobionts and the influence of the limiting factors: light, nutrients. The observed heterotrophic capability of a metalimnetic phytoplankton population might help explain the development in the deeper waters of Lyngbya contorta. The scheme of trophic interactions was put up, based on the assessment of the carbon pools and carbon flows in the pelagic zone of the lake. A mathematical model of the vertical structure of the lake's plankton populations was constructed, using the ecosystem description and data of vertical turbulent diffusion. The role of light and nutrient limitations and grazing mortality in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with the field observations, e.g. for the different patterns of the peaks, and for the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. Based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been theoretically discriminated for the first time. It has also been ascertained that irrespective of the depth the allochthonous substances limiting bacterial growth, the bacteria are uniformly distributed over depth and can serve as an indicator of the presence of limitation (the effect of autostabilisation in space). Of indisputable interest to limnology are the specific methods developed for understanding the functioning of Lake Shira ecosystem. These include the autostabilisation of the limiting factors, the on-the-spot fluorescent method of determining the three classes of microalgae, the algal mixotrophy and the planktonic population interactions and feedbacks, and development of a more sensitive, bioluminescent method for mapping the nonhomogeneities. Owing to a balanced combination of classical approaches (field observations, in situ data on production-decomposition) and the more recent ones (satellite monitoring, biophysical methods of estimating interactions of populations, mathematical models based on the field and experimental data), many of the structural-function relationships in the ecosystem can now be explained, and the models can provide 'mutual control and mutual agreement' between the data collected using different approaches.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Gulati, R.D.

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Study of the dielectric permeability in the superhigh frequency range of a degraded polyoxybutyrate biopolymer / G. A. Beliaev [и др.] // Biofizika. - 2000. - Vol. 45, Is. 4. - С. 636-640 . - ISSN 0006-3029
Кл.слова (ненормированные):
biopolymer -- hydroxybutyric acid -- Alcaligenes -- article -- chemistry -- electric conductivity -- radiofrequency radiation -- Alcaligenes -- Biopolymers -- Electric Conductivity -- Hydroxybutyrates -- Radio Waves
Аннотация: The dielectric permeability of the degradable biopolymer polyhydroxybutyrate synthesized by hydrogen-oxidizing bacteria Alcaligenes eutrophus was investigated by the resonance method using original highly sensitive microstrip sensors. For the first time, a linear growth of dielectric permeability (delta epsilon/delta T = 7 x 10(-4) degree-1) due to the flexibility of the polymer chain in the temperature range from 10 to 70 degrees C was revealed. The energy of a bend of the nearest fragments was evaluated (E = 392 K), and its correspondence to the energies of bends of the alcyl groups of low-molecular substances like liquid crystals was established. It was shown that at low values of dielectric permeability in the high-frequency range (epsilon' = 2.25 +/- 0.02), which are stable, in a wide range of frequencies of the electromagnetic field (1 MHz - 1 Hz), polyoxybutyrate can be used in the microwave equipment.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Beliaev, G.A.; Volova, T.G.; Drokin, N.A.; Shepov, V.N.

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Seasonal features of consumption of lysine by uncultivated bacterial plankton of Eutrophic water reservoir / M. Y. Trusova, O. V. Kolmakova, M. I. Gladyshev // Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - P391-398, DOI 10.1134/S1995425512040154 . - ISSN 1995-4255
Кл.слова (ненормированные):
16S ribosomal RNA -- biogeochemical function -- denaturing gradient gel electrophoresis -- eutrophic water reservoir -- lysine -- microecosystem (MES) -- uncultivated bacterial plankton -- amino acid -- bacterioplankton -- bacterium -- biogeochemistry -- electrokinesis -- eutrophication -- laboratory method -- microbial community -- polymerase chain reaction -- reservoir -- seasonal variation -- specialization -- Bacteria (microorganisms)
Аннотация: Dynamics of bacterial plankton community of eutrophic water reservoir in laboratory microecosystems with amino acid lysine was studied using PCR-DGGE technique. The addition of lysine to the microecosystems resulted in changes in the composition of the bacterial plankton in summer; in particular, a number of Lys1 and Lys2 species (genotypes) that consume this amino acid grew fast in the bacterial community. The plank tonic bacterial communities did not respond to the addition of lysine in spring and late summer. The obtained data confirm the hypothesis for the narrow specialization of bacterial plankton species to the consumption of individual organic substances. В© 2012 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok 50-50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnii prosp. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Y.; Kolmakova, O.V.; Gladyshev, M.I.

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Chemical Interactions between Planktonic Crustaceans / E. S. Zadereev // Zhurnal Obshchei Biologii. - 2002. - Vol. 63, Is. 2. - С. 166-167 . - ISSN 0044-4596
Кл.слова (ненормированные):
animal -- animal communication -- chemistry -- Crustacea -- ecosystem -- physiology -- phytoplankton -- plankton -- review -- zooplankton -- Animal Communication -- Animals -- Crustacea -- Ecosystem -- Phytoplankton -- Plankton -- Zooplankton
Аннотация: Three levels of chemical communications involved plankton Crustacea are considered: 1) Influence of zooplankton excretion on phytoplankton; 2) Influence of zooplankton excretion on the individuals of the same or other species of the same trophic level; 3) Influence of chemical cues released by predatory zooplankton and fish on herbivorous zooplankton. The data on the influence of excreted cues on some physiological (growth, reproduction, feeding, etc.) and behavioural (vertical and horizontal migrations) characters of planktonic crustaceans are presented. Ecological role and chemistry cues responsible for the interactions of different trophic levels can be different. It is considered that chemical communications in aquatic ecosystems can be provided with: 1) Species-specific cues that strictly influence particular biological functions (communication system of feromone type); 2) Non-specific cues that strictly influence particular functions (system of regulator, that act at the whole ecosystem as the hormonal system of an organism). 3) Non-specific substances with broad (non-specidic) influence - toxic substances of "biocondition substances" according to classification of Novikov and Kharlamova (2000).

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Science, Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zadereev, E.S.

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Chemical composition of water conditioned by crowded Moina macrocopa (Crustacea: Cladocera) / G. S. Kalacheva [et al.] // Russian Journal of Ecology. - 2000. - Vol. 31, Is. 2. - P113-117 . - ISSN 1067-4136
Кл.слова (ненормированные):
Chemical analysis -- Chemical composition -- Cladocera -- Metabolic products
Аннотация: The chemical composition of water conditioned by colonies of Moina macrocopa and involved in the regulation of their life cycle was analyzed in comparison with water samples from natural water bodies. Dechlorinated tap water was used as the control medium. The results showed that metabolism of crustaceans leads to an increase in the concentrations of phosphorus and nitrogen in all forms, the appearance of protein and carbohydrate fractions, and changes in the composition of lipid fractions in the water conditioned by their colonies. The contents of all organic components are very low, which makes their identification difficult. Concentrations of substances identified in the conditioned water do not exceed those occurring in nature. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zadereev, E.S.; Gubanov, V.G.; Gribovskaya, I.V.

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Use of human wastes oxidized to different degrees in cultivation of higher plants on the soil-like substrate intended for closed ecosystems / A. A. Tikhomirov [et al.] // Advances in Space Research. - 2010. - Vol. 46, Is. 6. - P744-750, DOI 10.1016/j.asr.2010.02.024 . - ISSN 0273-1177
Кл.слова (ненормированные):
Life support systems -- Microflora -- Mineralized human wastes -- Phototrophic unit -- Wet incineration -- Alternating electromagnetic field -- Bioregenerative life support systems -- Degree of oxidations -- Denitrifying microorganisms -- Growth and development -- Higher plants -- Human waste -- Life support systems -- Mass exchange -- Microbiotas -- Microflora -- Microscopic fungi -- Mineralized human wastes -- Nutrient solution -- Oxidation level -- Phytopathogenic bacteria -- Plant productivity -- Soil-like substrate -- Wheat plants -- Biomolecules -- Electromagnetic fields -- Irrigation -- Magnetic field effects -- Metabolism -- Metabolites -- Oxidation -- Plants (botany) -- Soils -- Solvent extraction -- Wastes -- Waste incineration
Аннотация: To close mass exchange loops in bioregenerative life support systems more efficiently, researchers of the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) have developed a procedure of wet combustion of human wastes and inedible parts of plants using H2O2 in alternating electromagnetic field. Human wastes pretreated in this way can be used as nutrient solutions to grow plants in the phototrophic unit of the LSS. The purpose of this study was to explore the possibilities of using human wastes oxidized to different degrees to grow plants cultivated on the soil-like substrate (SLS). The treated human wastes were analyzed to test their sterility. Then we investigated the effects produced by human wastes oxidized to different degrees on growth and development of wheat plants and on the composition of microflora in the SLS. The irrigation solution contained water, substances extracted from the substrate, and certain amounts of the mineralized human wastes. The experiments showed that the human wastes oxidized using reduced amounts of 30% H2O2: 1 ml/g of feces and 0.25 ml/ml of urine were still sterile. The experiments with wheat plants grown on the SLS and irrigated by the solution containing treated human wastes in the amount simulating 1/6 of the daily diet of a human showed that the degree of oxidation of human wastes did not significantly affect plant productivity. On the other hand, the composition of the microbiota of irrigation solutions was affected by the oxidation level of the added metabolites. In the solutions supplemented with partially oxidized metabolites yeast-like microscopic fungi were 20 times more abundant than in the solutions containing fully oxidized metabolites. Moreover, in the solutions containing incompletely oxidized human wastes the amounts of phytopathogenic bacteria and denitrifying microorganisms were larger. Thus, insufficiently oxidized sterile human wastes added to the irrigation solutions significantly affect the composition of the microbiological component of these solutions, which can ultimately unbalance the system as a whole. В© 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, 50/50, Krasnoyarsk 660036, Russian Federation
Universite Blaise Pascal, 24 avenue des Landais, 63174 Aubiere cedex, France
ESA/ESTEC, Keplerlaan 1, 2201 AZ Noordwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Kudenko, Y.A.; Ushakova, S.A.; Tirranen, L.S.; Gribovskaya, I.A.; Gros, J.-B.; Lasseur, C.

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Possibility of Salicornia europaea use for the human liquid wastes inclusion into BLSS intrasystem mass exchange / N. A. Tikhomirova [et al.] // Acta Astronautica. - 2008. - Vol. 63, Is. 7-10. - P1106-1110, DOI 10.1016/j.actaastro.2008.01.003 . - ISSN 0094-5765
Кл.слова (ненормированные):
BLSS -- NaCl turnover -- Salicornia europaea -- Space biology -- Acids -- Amides -- Arsenic compounds -- Biochemical engineering -- Biochemistry -- Biomass -- Cellulose -- Curing -- Drying -- Environmental engineering -- Fatty acids -- Garnets -- Health -- Human engineering -- Lipids -- Mineralogy -- Minerals -- Mining -- Nitrates -- Nitrogen -- Plants (botany) -- Polysaccharides -- Polyvinyl alcohols -- Renewable energy resources -- Silica -- Silicate minerals -- Sodium -- Sodium chloride -- Solutions -- Sugar (sucrose) -- Sugars -- Waste utilization -- Biochemical composition -- Biochemical substances -- Bioregenerative life support systems (BLSS) -- Component conditions -- Crude protein (CP) -- Dry weight (DW) -- Essential fatty acids -- Europaea -- H igh concentrations -- Human urine -- Linoleic acid (LA) -- Linolenic -- Lipid content -- Liquid wastes -- Mass exchanges -- Mineral compositions -- Nitrate nitrogen -- Nitrogen nutrition -- Non saturation -- Physico chemical processes -- Plant functions -- Plant lipids -- Reduced nitrogen -- Salicornia europaea -- Soluble sugars -- Wide spectrum -- Nonmetals -- Amides -- Arsenic -- Biochemistry -- Biomass -- Biotechnology -- Cellulose -- Curing -- Drying -- Fatty Acids -- Lipids -- Nonmetals -- Plants -- Polysaccharides -- Sugars
Аннотация: One of the ways of solving the problem of the human liquid wastes utilization in bioregenerative life support systems (BLSS) can be the use of halophytic vegetable plant Salicornia europaea capable of accumulating sodium chloride in rather high concentrations. Since the most specific higher plant function in BLSS, which at present cannot be substituted by physicochemical processes, appears to be the biosynthesis of a wide spectrum of nutritive substances necessary for a human, the object of the given work was the investigation of the S. europaea productivity, biochemical and mineral composition when grown under close to optimal BLSS vegetative component conditions. As the use of human urine after its preliminary physicochemical processing is supposed to be the mineral solution basis for the S. europaea cultivation, it is necessary to clear up the effect of reduced nitrogen on plants growth. Ground research was carried out. Biochemical composition of the S. europaea edible part showed that crude protein was contained in the highest degree. At that the content of crude protein (24% per dry weight) and cellulose (4.7% per dry weight) was higher in the plants grown on solutions containing amide nitrogen in comparison with the plants grown on solutions with nitrate nitrogen (15.4%-3.1% correspondingly). The water-soluble sugar contents were not high in the S. europaea edible part and depending on the nitrogen nutrition form they amounted to 1.1% (amide nitrogen) and 1.5% (nitrate nitrogen). The polysaccharide number (except cellulose) was rather higher and varied from 7.7% to 8.2%. Although the lipid content in the S. europaea plants was relatively low (7% per dry weight), it was shown that the plant lipids are characterized by a high nonsaturation degree mainly due to alpha linolenic and linoleic acids. Nitrogen nutrition form did not significantly affect the S. europaea productivity, and dry edible biomass of one plant was 8.6 g. Sodium and its concentrations predominated in the plant mineral composition and amounted in average to 9% per dry weight. Thus the S. europaea being the vegetable plant it can be the source of several biochemical substances and essential fatty acids. The present work also considers the influence of nitrate and amide forms of nitrogen on S. europaea biochemical and mineral composition. В© 2008 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
LGCB, Universite B. Pascal, CUST, BP206, 63174 Aubie're, cedex, France : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirova, N.A.; Ushakova, S.A.; Tikhomirov, A.A.; Kalacheva, G.S.; Gros, J.-B.

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Soil-like substrate for plant growing derived from inedible plant mass: Preparing, composition, fertility / J. -B. Gros [et al.] // Acta Horticulturae. - 2004. - Vol. 644. - P151-155
Кл.слова (ненормированные):
Fertility -- Hydroponics -- Oyster mushroom -- Phytomass yield -- Wheat straw -- Worms
Аннотация: Fertility of soil-like substrate (SLS) made by successive conversion of wheat straw by oyster mushrooms and worms has been evaluated. Soil-like substrate of different degree of maturity has been tested. The most ready SLS provided the higher yields of wheat. It comprised 9.5% of humic acids, 4.9% of fulvic acids and 15.2% of nonhydrolyzable substances. At atmospheric concentration of carbon dioxide the soil-like substrate decreased its mass over the vegetation period by 14- 21%. The yield of wheat, beans and cucumbers grown on the soil-like substrate was compared to that on hydroponics.

Scopus
Держатели документа:
LGCB, Universte B. Pascal, BP206, FR-63 174 Aubiere Cedex, France
ESA Estec, 2200 AG Noordwijk, Netherlands
Academgorodok, Institute of Biophysics, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gros, J.-B.; Lasseur, C.; Tikhomirov, A.A.; Manukovsky, N.S.; Ushakova, S.A.; Zolotukhin, I.G.; Gribovskaya, I.V.; Kovalev, V.S.

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10.

Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system / A. A. Tikhomirov [et al.] // Acta Astronautica. - 2003. - Vol. 53, Is. 4-10. - P249-257, DOI 10.1016/S0094-5765(03)00137-1 . - ISSN 0094-5765
Кл.слова (ненормированные):
Ecosystems -- Microorganisms -- pH -- Photosynthesis -- Plants (botany) -- Synthesis (chemical) -- Waste utilization -- Biological life support systems (BLLS) -- Gas exchange -- Plant respiration -- Biomass -- carbon dioxide -- Agaricales -- article -- biomass -- bioremediation -- growth, development and aging -- hydroponics -- incineration -- metabolism -- methodology -- microbiology -- microclimate -- photosynthesis -- plant physiology -- radish -- space flight -- waste management -- weightlessness -- wheat -- Agaricales -- Biodegradation, Environmental -- Biomass -- Carbon Dioxide -- Ecological Systems, Closed -- Environmental Microbiology -- Hydroponics -- Incineration -- Life Support Systems -- Photosynthesis -- Plant Physiology -- Raphanus -- Space Flight -- Triticum -- Waste Management -- Weightlessness
Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "biological incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Califomian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a man-made ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. В© 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Universite B. Pascal, Clermont-Ferrand, France
Environ. Contr. Life Support Sect., ESA, Estec Noonvijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Yu.A.; Koyalev, V.S.; Gribovskaya, I.V.; Tirranen, L.S.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, Ch.

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11.

Volatile metabolites of higher plant crops as a photosynthesizing life support system component under temperature stress at different light intensities / I. I. Gitelson [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1781-1786, DOI 10.1016/S0273-1177(03)00121-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Composition -- Crops -- Heat resistance -- Metabolites -- Photosynthesis -- Volatile metabolites -- Space research -- biosphere -- article -- comparative study -- gas -- growth, development and aging -- heat -- indoor air pollution -- light -- metabolism -- microclimate -- photon -- photosynthesis -- physiology -- radiation exposure -- volatilization -- wheat -- Air Pollution, Indoor -- Environment, Controlled -- Gases -- Heat -- Life Support Systems -- Light -- Photons -- Photosynthesis -- Triticum -- Volatilization
Аннотация: The effect of elevated temperatures of 35 and 45В°C (at the intensities of photosynthetically active radiation 322, 690 and 1104 ?mol-m-2-s-1) on the photosynthesis, respiration, and qualitative and quantitative composition of the volatiles emitted by wheat (Triticum aestuvi L., cultivar 232) crops was investigated in growth chambers. Identification and quantification of more than 20 volatile compounds (terpenoids - ?-pinene, ?3 carene, limonene, benzene, ?-and transcaryophyllene, ?- and ?-terpinene, their derivatives, aromatic hydrocarbons, etc.) were conducted by gas chromatograph/mass spectrometry. Under light intensity of 1104 ?mol-m-2-s-1, heat resistance of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. The action of elevated temperatures brought about variations in the rate and direction of the synthesis of volatile metabolites. The emission of volatile compounds was the greatest under a reduced irradiation of 322 ?mol-m-2-s-1 and the smallest under 1104 ?mol-m-2-s-1 at 35В°C. During the repair period, the contents and proportions of volatile compounds were different from their initial values, too. The degree of disruption and the following recovery of the functional state depended on the light intensity during the exposure to elevated temperatures. The investigation of the atmosphere of the growth chamber without plants has revaled the substances that were definitely technogenic in origin: tetramethylurea, dimethylsulfide, dibutylsulfide, dibutylphthalate, and a number of components of furan and silane nature. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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12.

Production process in Salicornia europaea plants as a prospective phototrophic constituent in bioregenerative life support systems / N. A. Tikhomirova, S. A. Ushakova, G. S. Kalacheva // Russian Journal of Plant Physiology. - 2009. - Vol. 56, Is. 1. - P22-28, DOI 10.1134/S102144370901004X . - ISSN 1021-4437
Кл.слова (ненормированные):
Ambient CO 2 -- Gas exchange -- Halophyte -- Macronutrients -- Productivity -- Salicornia europaea -- Salt tolerance -- Salicornia europaea
Аннотация: Plant productivity of a common glasswort Salicornia europaea L. was investigated in relation to the type of nitrogen nutrition and as a function of macronutrient concentrations mimicking the mineral composition of human urine. The source of nutrient nitrogen had no substantial effect on productivity of Salicornia europaea. In plants grown on media with amide as a nitrogen source, the content of nitrogenous substances, including glutamic and aspartic amino acids, was higher than in plants grown with nitrate. In plants grown on media with mineral composition analogous to that of human urine, the shoots accumulated Na and K in almost equally high amounts, on the background of high and nearly equal Na and K concentrations in the nutrient media. В© 2008 MAIK Nauka.

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirova, N.A.; Ushakova, S.A.; Kalacheva, G.S.

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13.

Bacterial bioluminescence and bioluminescent analysis / V. A. Kratasyuk, I. I. Gitel'zon // Biophysics. - 1982. - Vol. 27, Is. 6. - P977-995 . - ISSN 0006-3509
Аннотация: The principles of bioluminescent analysis and the current methods of quantitatively analysing different substances based on the use of bioluminescence of luminous bacteria and enzyme-substrate systems isolated from them are considered. The prospects of using bioluminescent methods in science, medicine and industry are discussed. В© 1983.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, U.S.S.R. Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Gitel'zon, I.I.

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14.

Biological-physical-chemical aspects of a human life support system for a lunar base / J. I. Gitelson [et al.] // Acta Astronautica. - 1995. - Vol. 37, Is. C. - P385-394 . - ISSN 0094-5765
Кл.слова (ненормированные):
animal -- aquaculture -- article -- biomass -- construction work and architectural phenomena -- Cyprinodontiformes -- filtration -- growth, development and aging -- human -- microbiology -- microclimate -- moon -- nutritional value -- photoperiodicity -- plant -- space flight -- standard -- Tilapia -- waste management -- water management -- wheat -- Animals -- Aquaculture -- Biomass -- Cyprinodontiformes -- Ecological Systems, Closed -- Facility Design and Construction -- Filtration -- Humans -- Life Support Systems -- Moon -- Nutritive Value -- Photoperiod -- Plants, Edible -- Space Flight -- Tilapia -- Triticum -- Waste Management -- Water Microbiology -- Water Purification
Аннотация: To create a life support system based on biological and physical-chemical processes is the optimum solution providing full-valued condidtions for existence and efficient work of people at a lunar base. Long-standing experinece in experimental research or closed ecosystems and their components allows us to suggest a realistic functional structure of the lunar base and to estimate qualitatively its parameters. The original restrictions are as follows: 1) the basic source of energy to support the biological processes has to be the solar radiation; 2) the initial amount of basic biological elelments forming the turnover of substances (C, O, H, P, K, N) has to be delivered from Earth; 3). Moon materials are not to be used in the biological turnover inside the base; 4) the base is to supply the crew fully with atmosphere and water, and with 90% (A scenario) or 40% (B scenario) of food. Experimental data about the plant productivity under the "Moon" rhythm of light and darkness allow us to suggest that the A scenario requires per one human: plant area - 40 m2 irradiated during the lunar day by 250-300 W/m2 PAR producing 1250 g of dry biomass a terrestrial day; a heterotrophic component of "biological incineration" of inedible plant biomass (800 g/day) including the aquaculture of fish to produce animal products and contaminating the environment less than birds and mammals, and the culture of edible mushrooms; a component of physical-chemical correction for the LSS envi ronment including the subsystems of: deep oxidation of organic impurities in the atmosphere and of water, organic wastes of human activity and that biological components (420 g/day) Co2 concentration in "Moon" nights, damping O2 in "Moon" days, etc. The stock of presotred or delivered from Earth substances (food additions, seeds, etc.) to be involved in biological turnover is to be about 50 kg/year per man. Increase of the mass of prestored substances per man up to 220 kg/year would reduce twice the plant area and consumed amount of radiant energy to exclude the components of "biological incineration" and physical-chemical destruction of organic wastes. В© 1995.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) Krasnoyarsk, Russian Federation
Ruhr-University of Bochum, C.E.B.A.S. Center of Excellence., Bochum, Germany
Institute of Medical-Biological Problems, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; V, B.; Grigoriev, A.I.; Lisovsky, G.M.; Manukovsky, N.S.; Sinyak, Y.u.E.; Ushakova, S.A.

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15.

Direct utilization of human liquid wastes by plants in a closed ecosystem / G. M. Lisovsky [et al.] // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1801-1804 . - ISSN 0273-1177
Кл.слова (ненормированные):
fertilizer -- nitrogen -- sodium chloride -- urea -- article -- biomass -- culture medium -- feasibility study -- human -- metabolism -- methodology -- microclimate -- sewage -- urine -- waste management -- wheat -- Biomass -- Culture Media -- Ecological Systems, Closed -- Feasibility Studies -- Fertilizers -- Humans -- Nitrogen -- Sodium Chloride -- Triticum -- Urea -- Urine -- Waste Disposal, Fluid -- Waste Management
Аннотация: Model experiments in phytotrons have shown that urea is able to cover 70% of the demand in nitrogen of the conveyer cultivated wheat. At the same time wheat plants can directly utilize human liquid wastes. In this article by human liquid wastes the authors mean human urine only. In a long-term experiment on "man-higher plants" system with two crewmen, plants covered 63 m2, with wheat planted to - 39.6 m2. For 103 days, complete human urine (total amount - 210.7 1) wassupplied into the nutrient solution for wheat. In a month and a half NaCl supply into the nutrient solution stabilized at 0.9-1.65 g/l. This salination had no marked effect on wheat production. The experiment revealed the realistic feasibility to directly involve liquid wastes into the biological turnover of the life support system. The closure of the system, in terms of water, increased by 15.7% and the supply of nutrients for wheat plants into the system was decreased. Closedness of biological turnover of matter in a man-made "man - higher plants" ecological system might involve, among other processes, direct utilization of human liquid wastes by plants. The amount of urine comprises 15-20% of the total amount of water cycling within the system including water as part of food, household, hygiene and potable water necessary for man. What is more, it they contains most nitrogen-bearing compounds emitted by man, almost all of the NaCl and some other substances involved in the biological turnover. Human liquid wastes can be utilized either by preliminary physical-chemical treatment (evaporating or freezing out the water, finally oxidizing the organic matter, isolating the mineral components required for plants, etc.) and further involvement of the obtained products or by direct application into the nutrient solution for plants. The challenge of direct utilization is that plants have no need of Na+ and Cl-, and also the organic forms of nitrogen emitted by man cannot fully meet the demand of plants forthis element. Besides, hygienic and/or psychological reasons make it desirable to avoid direct use of liquid wastes in the nutrient solutions that would have direct contact with edible part of plants (tubers, roots, bulbs). Feasibility of direct utilization of liquid wastes by plants in a closed "man - higher plants" ecosystem has been experimentally studied on wheat - grain culture as a model plant with the edible part in the form of seeds spatially dissociated with the nutrient medium. The wheat covered 60-65% of the area under higher plants. The studies have been carried out in "Bios-3"experimental facility described in detail elsewhere (Lisovsky, 1979; Gitelson et al., 1989). В© 1997 COSPAR. Published by Elsevier Science Ltd.

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Держатели документа:
Inst. Biophys.-Russ. Acad. of Sci., Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lisovsky, G.M.; Gitelson, J.I.; Shilenko, M.P.; Gribovskaya, I.V.; Trubachev, I.N.

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16.

Impaired growth of plants cultivated in a closed system: Possible reasons / J. I. Gitelson [et al.] // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1927-1930 . - ISSN 0273-1177
Кл.слова (ненормированные):
air conditioning -- air pollutant -- article -- culture medium -- growth, development and aging -- human -- hydroponics -- methodology -- microbiology -- microclimate -- plant -- sewage -- wheat -- Air Conditioning -- Air Pollutants -- Culture Media -- Ecological Systems, Closed -- Environment, Controlled -- Humans -- Hydroponics -- Life Support Systems -- Plants -- Triticum -- Waste Disposal, Fluid -- Water Microbiology
Аннотация: Plants in experiments on "man-higher plants" closed ecosystem (CES) have been demonstrated to have inhibited growth and reduced productivity due to three basic factors: prolonged usage of a permanent nutrient solution introduction into the nutrient medium of intra-system gray water, and closure of the system. Gray water was detrimental to plants the longer the nutrient solution was used. However, higher plant growth was mostly affected by the gaseous composition of the CES atmosphere, through accumulation of volatile substances. В© 1997 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; Tirranen, L.S.; Borodina, E.V.; Rygalov, V.Ye.

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17.

Man as a component of a closed ecological life support system / J. I. Gitelson, U - Okladnikov YuN // Life support & biosphere science : international journal of earth space. - 1994. - Vol. 1, Is. 2. - P73-81 . - ISSN 1069-9422
Кл.слова (ненормированные):
carbon dioxide -- oxygen -- article -- blood analysis -- blood cell count -- breathing -- construction work and architectural phenomena -- crop -- diet -- growth, development and aging -- human -- metabolism -- microclimate -- physiology -- Russian Federation -- space flight -- standard -- waste management -- water supply -- Blood Cell Count -- Blood Chemical Analysis -- Carbon Dioxide -- Crops, Agricultural -- Diet -- Ecological Systems, Closed -- Environment, Controlled -- Facility Design and Construction -- Humans -- Life Support Systems -- Oxygen -- Respiration -- Russia -- Space Flight -- Waste Management -- Water Supply
Аннотация: Material support of all manned space flights so far has been provided from a prestored stock of substances or replenished from the Earth's biosphere. Exploration of space will, however, become real only when man is able to break away from Earth completely, when he will be accompanied by a system providing everything necessary to sustain full-valued life for an unlimited time. The only known system to date meeting this requirement is the Earth's biosphere. To break away from his cradle, as K.E. Tsiolkovsky called Earth, it is necessary to devise a life support system functionally similar to the natural biosphere. This need not be similar in structure to the vast diversity of trophic relationships available on Earth, but requires the solution of a multitude of various problems of an ecological, physiological, engineering and social-psychological nature. Human life-support systems based on biological regeneration of environments in small volumes have been studied at the Institute of Biophysics (Siberian Branch of the Russian Academy of Sciences) over many years. This work has resulted in the design of Bios-3, a biologically-based self-sustained human life support system.

Scopus
Держатели документа:
Institute of Biophysics (Siberian Branch of Russian Academy of Sciences), Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; , U - Okladnikov YuN

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18.

"Biospherics" approach for studies of natural and artificial ecosystems / N. S. Pechurkin, L. A. Somova // Advances in Space Research. - 2008. - Vol. 41, Is. 5. - P691-695, DOI 10.1016/j.asr.2007.09.021 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biospherics -- Biotic turnover -- Limiting substance -- Natural and artificial ecosystems -- Biogeochemistry -- Ecosystems -- Mathematical models -- Artificial ecosystems -- Energy fluxes -- Biospherics
Аннотация: The main unifying feature of natural and artificial ecosystems is their biotic turnover (cycling) of substances which is induced with energy fluxes. A new integrating scientific discipline - Biospherics - studies biotic cycles (both in experiments and in mathematical models) of different degree of closure and complexity. By its origin, Biospherics is to be connected with extensive studies of Biosphere by Russian academician Vladimir Vernadsky. He developed and used "empirical generalizations" based on innumerous observations, comparisons and reflections. His "bio-geo-chemical principles" of Biosphere and ecosystems development have more qualitative than quantitative nature. Quantitative criteria to evaluate the efficiency of natural and artificial ecosystems are to take into account energy fluxes and their use in ecosystems of different types. At least, three of them are of value for estimation of natural and artificial ecosystems' functional activities. Energy principle of extensive development (EPED), energy principle of intensive development (EPID) and main universal (generalized) criterion (MUC). The last criterion (Principle) characterizes the specific cycling rate of limiting chemical elements in multi-organism systems, developing under external energy fluxes. Its value can be a quantitative measure of effectiveness for every ecosystem functioning, including our global Biosphere. Different examples of these (above-mentioned) integrated criteria actions are presented and analyzed in the paper. В© 2007 COSPAR.

Scopus
Держатели документа:
Institute of Biophysics SB, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Somova, L.A.

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19.

Quantitative criteria for estimation of natural and artificial ecosystems functioning / N. S. Pechurkin // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1507-1511, DOI 10.1016/j.asr.2005.01.059 . - ISSN 0273-1177
Кл.слова (ненормированные):
Artificial ecosystems -- Limiting substances -- Natural ecosystems -- Quantitative criteria -- Carbon dioxide -- Energy management -- Estimation -- Hierarchical systems -- Personnel -- Plants (botany) -- Reliability -- Artificial ecosystems -- Limiting substances -- Natural ecosystems -- Quantitative criteria -- Ecosystems -- carbon -- biomass -- chemistry -- conference paper -- ecology -- ecosystem -- energy transfer -- microclimate -- photosynthesis -- population dynamics -- Biomass -- Carbon -- Ecological Systems, Closed -- Ecology -- Ecosystem -- Energy Transfer -- Life Support Systems -- Photosynthesis -- Population Dynamics
Аннотация: Using biotic turnover of substances in trophic chains, natural and artificial ecosystems are similar in functioning, but different in structure. It is necessary to have quantitative criteria to evaluate the efficiency of artificial ecosystems (AES). These criteria are dependent on the specific objectives for which the AES are designed. For example, if AES is considered for use in space, important criteria are efficiency in use of mass, power, volume (size) and human labor and reliability. Another task involves the determination of quantitative criteria for the functioning of natural ecosystems. To solve the problem, it is fruitful to use a hierarchical approach suitable for both individual links and the ecosystem as a whole. Energy flux criteria (principles) were developed to estimate the functional activities of biosystems at the population, community and ecosystem levels. A major feature of ecosystems as a whole is their biotic turnover of matter the rate of which is restricted by the lack of limiting substances. Obviously, the most generalized criterion is to take into account the energy flux used by the biosystem and the quantity of limiting substance included in its turnover. The use of energy flux by ecosystem, EUSED - is determined from the photoassimilation of CO2 by plants (per time unit). It can be approximately estimated as the net primary production of photosynthesis (NPP). So, the ratio of CO 2 photoassimilation rate (sometimes, measured as NPP) to the total mass of limiting substrate can serve as a main universal criterion (MUC). This MUC characterizes the specific cycling rate of limiting chemical elements in the system and effectiveness of every ecosystem including the global Biosphere. Comparative analysis and elaboration of quantitative criteria for estimation of natural and artificial ecosystems activities is of high importance both for theoretical considerations and for real applications. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.

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20.

Principles of biological adaptation of organisms in artificial ecosystems to changes of environmental factors / L. A. Somova, N. S. Pechurkin, T. I. Pisman // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1512-1515, DOI 10.1016/j.asr.2004.12.038 . - ISSN 0273-1177
Кл.слова (ненормированные):
Artificial ecosystems -- Biotic cycles -- Microorganisms -- Energy utilization -- Environmental impact -- Microorganisms -- Plants (botany) -- Purification -- Water -- AES -- Artificial ecosystems -- Biotic cycles -- Material transformations -- Ecosystems -- bioremediation -- comparative study -- conference paper -- ecosystem -- methodology -- microbiology -- microclimate -- plant -- sewage -- waste management -- water management -- Biodegradation, Environmental -- Ecological Systems, Closed -- Ecosystem -- Environmental Microbiology -- Life Support Systems -- Plants -- Sewage -- Soil Microbiology -- Waste Management -- Water Purification
Аннотация: Studying material transformations and biotic cycling in artificial ecosystems (AES), we need to know the principles of biological adaptation of active organisms to change in the environment. Microorganisms in AES for water purification are the most active transforming organisms and consumers of the organic substances contained in wastes. Utilization of organic substances is directly connected with the energy fluxes used by AES. According to energy criteria, the energy fluxes used by a biological system tend to reach maximum values under stable conditions. Unutilized substrate concentration decreases as a result of biological adaptations. After a dramatic change in environmental factors, for example, after a sharp increase in the flow rate of organic substances, the biological system is not able to react quickly. The concentration of unutilized substrate increases and the energy flux used by the biological system decreases. The structure of the microbial community also changes, with a decrease in biological diversity. The efficiency of energy use by simple terrestrial ecosystems depends on the energetic intensity and interactions between plants and rhizospheric microorganisms. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk 36, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Pisman, T.I.

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