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Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562 / T. G. Volova [et al.] // Applied Microbiology and Biotechnology. - 2006. - Vol. 73, Is. 3. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Biochemistry -- Carbon -- Genetic engineering -- Glucose -- Hydrogen -- Polyesters -- Cultural properties -- Glucose-utilizing mutant strain -- Nitrogen deficiency -- Polyhydroxyalkanoate (PHA) -- Bacteria -- 3 hydroxybutyric acid -- carbon dioxide -- fructose -- glucose -- hydrogen -- monomer -- polyhydroxyalkanoic acid -- sugar -- bacterium -- carbon -- comparative study -- energy -- ester -- experimental study -- glucose -- hydrogen -- physicochemical property -- article -- bacterial strain -- bacterium culture -- bacterium mutant -- biomass -- chemical analysis -- Cupriavidus necator -- energy consumption -- genetic analysis -- nonhuman -- synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO2, fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is ?-hydroxybutyric acid (more than 99 mol %); the identified minor components are ?-hydroxyvaleric acid (0.25-0.72 mol %) and ?-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose. В© Springer-Verlag 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. / T. G. Volova [et al.] // Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
beta hydroxyvaleric acid -- beta-hydroxyvaleric acid -- carbon dioxide -- ester -- fatty acid -- fructose -- glucose -- hexanoic acid derivative -- hydrogen -- hydroxyacid -- oxygen -- polymer -- ribosome RNA -- valeric acid derivative -- article -- biotechnology -- chemistry -- metabolism -- methodology -- Wautersia eutropha -- Biotechnology -- Carbon Dioxide -- Cupriavidus necator -- Esters -- Fatty Acids -- Fructose -- Glucose -- Hexanoic Acids -- Hydrogen -- Hydroxy Acids -- Oxygen -- Pentanoic Acids -- Polymers -- RNA, Ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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Possibility of Salicornia europaea use for the human liquid wastes inclusion into BLSS intrasystem mass exchange / N. A. Tikhomirova [et al.] // Acta Astronautica. - 2008. - Vol. 63, Is. 7-10. - P1106-1110, DOI 10.1016/j.actaastro.2008.01.003 . - ISSN 0094-5765
Кл.слова (ненормированные):
BLSS -- NaCl turnover -- Salicornia europaea -- Space biology -- Acids -- Amides -- Arsenic compounds -- Biochemical engineering -- Biochemistry -- Biomass -- Cellulose -- Curing -- Drying -- Environmental engineering -- Fatty acids -- Garnets -- Health -- Human engineering -- Lipids -- Mineralogy -- Minerals -- Mining -- Nitrates -- Nitrogen -- Plants (botany) -- Polysaccharides -- Polyvinyl alcohols -- Renewable energy resources -- Silica -- Silicate minerals -- Sodium -- Sodium chloride -- Solutions -- Sugar (sucrose) -- Sugars -- Waste utilization -- Biochemical composition -- Biochemical substances -- Bioregenerative life support systems (BLSS) -- Component conditions -- Crude protein (CP) -- Dry weight (DW) -- Essential fatty acids -- Europaea -- H igh concentrations -- Human urine -- Linoleic acid (LA) -- Linolenic -- Lipid content -- Liquid wastes -- Mass exchanges -- Mineral compositions -- Nitrate nitrogen -- Nitrogen nutrition -- Non saturation -- Physico chemical processes -- Plant functions -- Plant lipids -- Reduced nitrogen -- Salicornia europaea -- Soluble sugars -- Wide spectrum -- Nonmetals -- Amides -- Arsenic -- Biochemistry -- Biomass -- Biotechnology -- Cellulose -- Curing -- Drying -- Fatty Acids -- Lipids -- Nonmetals -- Plants -- Polysaccharides -- Sugars
Аннотация: One of the ways of solving the problem of the human liquid wastes utilization in bioregenerative life support systems (BLSS) can be the use of halophytic vegetable plant Salicornia europaea capable of accumulating sodium chloride in rather high concentrations. Since the most specific higher plant function in BLSS, which at present cannot be substituted by physicochemical processes, appears to be the biosynthesis of a wide spectrum of nutritive substances necessary for a human, the object of the given work was the investigation of the S. europaea productivity, biochemical and mineral composition when grown under close to optimal BLSS vegetative component conditions. As the use of human urine after its preliminary physicochemical processing is supposed to be the mineral solution basis for the S. europaea cultivation, it is necessary to clear up the effect of reduced nitrogen on plants growth. Ground research was carried out. Biochemical composition of the S. europaea edible part showed that crude protein was contained in the highest degree. At that the content of crude protein (24% per dry weight) and cellulose (4.7% per dry weight) was higher in the plants grown on solutions containing amide nitrogen in comparison with the plants grown on solutions with nitrate nitrogen (15.4%-3.1% correspondingly). The water-soluble sugar contents were not high in the S. europaea edible part and depending on the nitrogen nutrition form they amounted to 1.1% (amide nitrogen) and 1.5% (nitrate nitrogen). The polysaccharide number (except cellulose) was rather higher and varied from 7.7% to 8.2%. Although the lipid content in the S. europaea plants was relatively low (7% per dry weight), it was shown that the plant lipids are characterized by a high nonsaturation degree mainly due to alpha linolenic and linoleic acids. Nitrogen nutrition form did not significantly affect the S. europaea productivity, and dry edible biomass of one plant was 8.6 g. Sodium and its concentrations predominated in the plant mineral composition and amounted in average to 9% per dry weight. Thus the S. europaea being the vegetable plant it can be the source of several biochemical substances and essential fatty acids. The present work also considers the influence of nitrate and amide forms of nitrogen on S. europaea biochemical and mineral composition. В© 2008 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
LGCB, Universite B. Pascal, CUST, BP206, 63174 Aubie're, cedex, France : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirova, N.A.; Ushakova, S.A.; Tikhomirov, A.A.; Kalacheva, G.S.; Gros, J.-B.

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Effect of NaCl concentration on productivity and mineral composition of Salicornia europaea as a potential crop for utilization NaCl in LSS / S. A. Ushakova [et al.] // Advances in Space Research. - 2005. - Vol. 36, Is. 7. - P1349-1353, DOI 10.1016/j.asr.2004.09.017 . - ISSN 0273-1177
Кл.слова (ненормированные):
Life support system -- NaCl -- Salicornia europaea -- Space biology -- Calcium -- Concentration (process) -- Minerals -- Photosynthesis -- Plants (botany) -- Salinity measurement -- Sodium chloride -- Vegetation -- Life support systems -- Liquid wastes -- NaCl -- Salicornea europea -- Space biology -- Space research
Аннотация: The accumulation of solid and liquid wastes in manmade ecosystems presents a problem that has not been efficiently solved yet. Urine, containing NaCl, are part of these products. This is an obstacle to the creation of biological systems with a largely closed material cycling, because the amount of solid and liquid wastes in them must be reduced to a minimum. A possible solution to the problem is to select plant species capable of utilizing sufficiently high concentrations of NaCl, edible for humans, and featuring high productivity. Until recently, the life support systems have included the higher plants that were either sensitive to salinization (wheat, many of the legumes, carrot, potato, maize) or relatively salt-resistant (barley, sugar beet, spinach). Salicomia europaea, whose above-ground part is fully edible for humans, is one of the most promising candidates to be included in life support systems. It is reported in the literature that this plant is capable of accumulating up to 50% NaCl (dry basis). Besides, excessive accumulation of sodium ions should bring forth a decrease in the uptake of potassium ions and other biogenic elements. The aim of this work is to study the feasibility of using S. europaea plants in growth chambers to involve NaCl into material cycling. Plants were grown in vegetation chambers at the irradiance of 100 or 150 W/m2 PAR (photosynthetically active radiation) and the air temperature 24 В°C, by two methods. The first method was to grow the plants on substrate - peat. The peat was supplemented with either 3% NaCl (Variant 1) or 6% NaCl (Variant 2) of the oven-dry mass of the peat. The second method was to grow the plants in water culture, using the solution with a full complement of nutrients, which contained 0.0005% of NaCl, 1% or 2%. The study showed that the addition of NaCl to the substrate or to the solution resulted in the formation of more succulent plants, which considerably increased their biomass. The amount of NaCl uptake was the highest in the plants grown in water culture, 2.6 g per plant. As the sodium uptake increased, the consumption of potassium and the sum of the reduced N forms decreased twofold. The uptake of calcium and magnesium by plants decreased as the NaCl concentration increased; the smallest amounts were taken up by S. europaea grown in water culture. Salinity had practically no effect on the uptake of phosphorus and sulfur. Thus, S. europaea is a promising candidate to be included in life support systems; of special interest is further research on growing these plants in water culture. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Science, Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Kovaleva, N.P.; Gribovskaya, I.V.; Dolgushev, V.A.; Tikhomirova, N.A.

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Pine Stands as Bioindicators: Justification for Air Toxicity Monitoring in an Industrial Metropolis / G. Polyakova, N. Pashenova, V. Senashova [et al.] // Environments. - 2020. - Vol. 7, Is. 4. - Ст. 28, DOI 10.3390/environments7040028. - Cited References:35. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, projects 0356-2018-0742 and 0356-2017-0017, and by RFBR grant 15-04-06575, RFBR-Krasnoyarsk Regional Foundation N 18-44-240004, grant 18-44-242002. . - ISSN 2076-3298

РУБ Environmental Sciences
Рубрики:
HORMESIS
Кл.слова (ненормированные):
environmental monitoring -- pollutants -- toxicity -- creeping fire -- high-grade pine stands -- bioindicators
Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middle-aged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich's model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye's adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown.

WOS
Держатели документа:
FRC KSC SB RAS, Sukachev Inst Forest SB RAS, Krasnoyarsk 660036, Russia.
Nonstate Educ Inst Higher Profess Educ Siberian I, Krasnoyarsk 660069, Russia.
FRC KSC SB RAS, Photobiol Lab, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Polyakova, Galina; Pashenova, Natalia; Senashova, Vera; Podolyak, Natalia; Kudryasheva, Nadezhda; Russian Academy of SciencesRussian Academy of Sciences [0356-2018-0742, 0356-2017-0017]; RFBRRussian Foundation for Basic Research (RFBR) [15-04-06575]; RFBR-Krasnoyarsk Regional Foundation [18-44-240004, 18-44-242002]

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Pine stands as bioindicators: Justification for air toxicity monitoring in an industrial metropolis / G. Polyakova, N. Pashenova, V. Senashova [et al.] // Environ. - MDPI. - 2020. - Vol. 7, Is. 4. - Ст. 28, DOI 10.3390/environments7040028 . - ISSN 2076-3298
Кл.слова (ненормированные):
Bioindicators -- Creeping fire -- Environmental monitoring -- High-grade pine stands -- Pollutants -- Toxicity
Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middleaged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich‘s model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye‘s adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown. © 2020 by the authors.

Scopus
Держатели документа:
Sukachev Institute of Forest SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Non-State Educational Institution of Higher Professional Education Siberian Institute of Business, Management and Psychology, Krasnoyarsk, 660069, Russian Federation
Photobiology Lab, Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Biophysics Department, Siberian Federal University, Krasnoyarsk, 660049, Russian Federation

Доп.точки доступа:
Polyakova, G.; Pashenova, N.; Senashova, V.; Podolyak, N.; Kudryasheva, N.

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A New Bias Site for Epigenetic Modifications: How Non-Canonical GC Base Pairs Favor Mechanochemical Cleavage of DNA / D. A. Semyonov, I. V. Eltsov, Y. D. Nechipurenko // Bioessays. - 2020. - Vol.: 42, Issue : 11. - Ст. 2000051, DOI 10.1002/bies.202000051. - Cited References:55. - The authors thank Dr. Dmitry M. Graifer, Prof. Vladimir A. Reznikov, and Dr. Anastasia Anashkina, for useful discussion. The authors thank the Prof. D. R. Forsdyke whose advice on improving the style of the manuscript was very useful. This work was supported by the program of the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818. The work was supported by the Russian Ministry of Science and High Education (grant #05.604.21.0234, unique number RFMEFI60419X0234) . - ISSN 0265-9247. - ISSN 1521-1878

РУБ Biochemistry & Molecular Biology + Biology
Рубрики:
NUCLEIC-ACIDS
   CYTOSINE
   MUTAGENESIS
   N-4-METHOXYCYTOSINE
Кл.слова (ненормированные):
5-methylcytosines -- mechanochemical cleavage of DNAs -- molecular basis of -- epigenetics -- tautomers -- transient base pairs in DNAs -- wobble base pairs
Аннотация: Properties of non-canonical GC base pairs and their relations with mechanochemical cleavage of DNA are analyzed. A hypothesis of the involvement of the transient GC wobble base pairs both in the mechanisms of the mechanochemical cleavage of DNA and epigenetic mechanisms involving of 5-methylcytosine, is proposed. The hypothesis explains the increase in the frequency of the breaks of the sugar-phosphate backbone of DNA after cytosines, the asymmetric character of these breaks, and an increase in break frequency in CpG after cytosine methylation. As an alternative hypothesis, probable implication of GC(+)Hoogsteen base pairs is considered, which now exemplify the best-studied non-canonical GC base pairs in the DNA double helix. Also see the video abstract here https://youtu.be/EUunVWL0ptw.

WOS,
Файл:  доступ к статье - 0
https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.202000051
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Akademgorodok 50, Krasnoyarsk 660036, Russia.
Novosibirsk State Univ, Novosibirsk, Russia.
Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow, Russia.

Доп.точки доступа:
Semyonov, Denis A.; Eltsov, Ilia, V; Nechipurenko, Yury D.; program of the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]; Russian Ministry of Science and High Education [05.604.21.0234, RFMEFI60419X0234]

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Efficacy of embedded metribuzin and tribenuron-methyl herbicides in field-grown vegetable crops infested by weeds / T. Volova, A. Demidenko, N. Kurachenko [et al.] // Environ. Sci. Pollut. Res. - 2020, DOI 10.1007/s11356-020-10359-1. - Cited References:35. - This work was supported by Project "Agropreparations of the new generation: a strategy of construction and realization" [Agreement No 074-02-2018-328] in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning". . - Article in press. - ISSN 0944-1344. - ISSN 1614-7499

РУБ Environmental Sciences
Рубрики:
DEFICIT IRRIGATION
   CONTROLLED-RELEASE
   YIELD
   TOMATO
   FORMULATIONS
Кл.слова (ненормированные):
Metribuzin -- Tribenuron-methyl -- Embedding -- Degradable P(3HB) -- Weed growth -- inhibition -- Quality of tomato fruits and beet roots
Аннотация: The purpose of the present study was to investigate the efficacy of the experimental formulations of the metribuzin (MET) and tribenuron-methyl (TBM) herbicides embedded in the matrix of degradable poly-3-hydroxybutyrate blended with wood flour in field-grown tomato and beet crops infested by weeds. There is a necessity to develop environmentally friendly and effective means to protect plants because of the shortcomings of the free herbicide forms such as the environmentally unsafe spray application of solutions and suspensions of the widespread metribuzin and tribenuron-methyl herbicides, removal from soil during watering events and rains, and transport to natural aquatic environments, where the herbicides accumulate in the trophic chains of biota. Free TBM is also rapidly inactivated in soil and metabolized to nontoxic products in plants. The efficacy of experimental formulations of metribuzin and tribenuron-methyl embedded in the matrix of degradable poly-3-hydroxybutyrate blended with wood flour was tested in field-grown tomato and beet crops infested with weeds. Application of metribuzin resulted in the highest productivity of tomatoes (2.3 kg/m(2)) and table beet (3.4 kg/m(2)), improved biometric parameters of tomato fruits and beet roots, and caused reduction in nitrate nitrogen concentrations in them. The mode of herbicide delivery did not affect sugar contents, but application of both metribuzin and tribenuron-methyl induced a 1.7-fold and 1.4-fold, respectively, increase in vitamin C concentrations in tomato fruits and beet roots relative to the vegetables grown on the subplots treated with free herbicides and the intact plants. Embedded herbicides can be used as preemergence herbicides in the field.

WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk State Agr Univ, 90 Mir Av, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Volova, Tatyana; Demidenko, Alexey; Kurachenko, Natalia; Baranovsky, Sergey; Petrovskaya, Olga; Shumilova, Anna; Project "Agropreparations of the new generation: a strategy of construction and realization" of the Government of the Russian Federation [074-02-2018-328]

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