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Model assessment of the migration capability of transuranium radionuclides (241Am and Pu isotopes) and 152Eu in the system bottom sediments-Yenisei river water by chemical fractionation technique / L. G. Bondareva [et al.] // Radiochemistry. - 2005. - Vol. 47, Is. 4. - P415-421, DOI 10.1007/s11137-005-0112-8 . - ISSN 1066-3622
Аннотация: Sorption of radiotracers 241Am and 242Pu and weighable amounts of uranium and stable Eu on bottom sediments in the simulated system bottom sediments-Yenisei river water was studied. At a contact time of 40 min these metals are completely sorbed on the bottom sediments, presumably via association with organomineral complexes and hydrated gels. Along with sorption, these metals can form soluble complexes and stable colloids (pseudocolloids). The distribution factors of 241Am and 242Pu, and stable Eu in the simulated system between bottom sediments and liquid phases were calculated from the results of sorption experiments. The introduced 241Am and 152Eu initially present in radioactively contaminated bottom sediments of the Yenisei river show similar pattern of distribution over differently mobile fractions. The distribution of weighable amounts of stable Eu significantly differs from that of initially present 152Eu and tracer 241Am due to decelerated sorption of stable Eu and its considerably higher concentration. A significant part of 242Pu, 241Am, and 152Eu is associated with mobile fractions of bottom sediments, which, under certain environmental conditions, can pass into river water as migrating species. В©2005 Pleiades Publishing, Inc.

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Joint Institute of Geology, Geophysics, and Mineralogy, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russian Federation
Institute of Chemistry and Chemical Technology, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.G.; Bolsunovskii, A.Ya.; Sukhorukov, F.V.; Kazbanov, V.I.; Makarova, I.V.; Legler, E.V.

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Assessment of the rate of accumulation of the transuranium element americium-241 by the aquatic plant Elodea canadensis / A. Ya. Bolsunovskii [et al.] // Doklady Biological Sciences. - 2004. - Vol. 399, Is. 1-6. - P467-469, DOI 10.1007/s10630-005-0014-0 . - ISSN 0012-4966
Кл.слова (ненормированные):
americium -- radioisotope -- water -- article -- biomass -- drug effect -- environmental monitoring -- Hydrocharitaceae -- methodology -- pH -- plant -- plant root -- radiation exposure -- radiochemistry -- time -- toxicity testing -- water pollutant -- Americium -- Biomass -- Environmental Monitoring -- Hydrocharitaceae -- Hydrogen-Ion Concentration -- Plant Roots -- Plant Shoots -- Plants -- Radiochemistry -- Radioisotopes -- Time Factors -- Toxicity Tests -- Water -- Water Pollutants, Radioactive -- Elodea canadensis

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Ya.; Zotina, T.A.; Bondareva, L.G.; Degermendzhi, A.G.

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Evidence of the radioactive fallout in the center of asia (russia) following the fukushima nuclear accident / A. Bolsunovsky, D. Dementyev // Journal of Environmental Radioactivity. - 2011. - Vol. 102, Is. 11. - P1062-1064, DOI 10.1016/j.jenvrad.2011.06.007 . - ISSN 0265-931X
Кл.слова (ненормированные):
Environmental samples -- Fission product radionuclides -- Fukushima nuclear accident -- Radioactive fallout -- Russia -- Chernobyl accident -- Environmental sample -- Fallout radionuclides -- Global effects -- High velocity -- Nuclear accidents -- Radioactive contamination -- Russia -- Water samples -- Cesium -- Fallout -- Fission products -- Radioactivity -- Radioisotopes -- Nuclear reactor accidents -- cesium 137 -- iodine 131 -- rain -- atmospheric pollution -- cesium isotope -- fallout -- nuclear accident -- radioactive pollution -- radionuclide -- article -- environmental exposure -- gamma spectrometry -- nuclear accident -- radioactive contamination -- radioactive waste -- water analysis -- water contamination -- Cesium Radioisotopes -- Cities -- Humans -- Iodine Radioisotopes -- Japan -- Radiation Dosage -- Radiation Monitoring -- Radioactive Fallout -- Radioactive Hazard Release -- Radioactive Pollutants -- Risk Assessment -- Russia -- Time Factors -- Fukushima -- Honshu -- Japan -- Krasnoyarsk [Russian Federation] -- Russian Federation -- Tohoku
Аннотация: It was recently reported that radioactive fallout due to the Fukushima Nuclear Accident was detected in environmental samples collected in the USA and Greece, which are very far away from Japan. In April-May 2011, fallout radionuclides ( 134Cs, 137Cs, 131I) released in the Fukushima Nuclear Accident were detected in environmental samples at the city of Krasnoyarsk (Russia), situated in the center of Asia. Similar maximum levels of 131I and 137Cs/ 134Cs and 131I/ 137Cs ratios in water samples collected in Russia and Greece suggest the high-velocity movement of the radioactive contamination from the Fukushima Nuclear Accident and the global effects of this accident, similar to those caused by the Chernobyl accident. В© 2011 Elsevier Ltd.

Scopus
Держатели документа:
Radioecology Laboratory, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 50-50, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Dementyev, D.

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Accumulation of 242Pu by a macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments / A. Bolsunovsky [et al.] // Chemosphere. - 2009. - Vol. 75, Is. 3. - P284-288, DOI 10.1016/j.chemosphere.2008.12.036 . - ISSN 0045-6535
Кл.слова (ненормированные):
242Pu -- Accumulation -- Laboratory experiments -- Sequential extraction technique -- Submerged plant Elodea canadensis -- Yenisei River -- sup242/supPu -- Accumulation -- Laboratory experiments -- Sequential extraction technique -- Submerged plant Elodea canadensis -- Yenisei River -- Biological materials -- Biomass -- Chemical plants -- Experiments -- Positive ions -- Rivers -- Plutonium -- concentration (composition) -- experimental study -- laboratory method -- macrophyte -- plutonium isotope -- river water -- submerged vegetation -- Eurasia -- Russian Federation -- Yenisei River -- plutonium -- article -- biomass -- chemistry -- Hydrocharitaceae -- physiology -- plant -- radiation monitoring -- river -- time -- water pollutant -- Biomass -- Hydrocharitaceae -- Plant Shoots -- Plutonium -- Radiation Monitoring -- Rivers -- Time Factors -- Water Pollutants, Radioactive
Аннотация: The study addresses 242Pu accumulation by Elodea canadensis, one of the abundant species of submerged plants in the Yenisei River. 242Pu in water samples of the "Elodea - Yenisei River water" model system and in the biomass fractions was determined ?-spectrometrically, following radiochemical recovery of 242Pu using 236Pu - a chemical yield tracer. The experiments on accumulation of 242Pu by Elodea biomass showed that the activity concentration of 242Pu can reach 21 В± 2 Bq/g dry wt, with the concentration factor for 242Pu 13100 В± 2100 L/kg dry wt. Results of chemical fractionation proved that during the first few hours of the experiment 242Pu contained in Elodea was mainly concentrated in the exchangeable and the adsorbed fractions of biomass (about 100%). As Elodea biomass accumulated 242Pu, the absolute amount of 242Pu in the exchangeable and the adsorbed fractions remained almost unchanged, although the portion of 242Pu tightly bound to biomass increased. At the end of the experiment, on day 7, 242Pu tightly bound to biomass (fractions of organics and mineral residue) constituted 43-63% (in different experiments) of the total 242Pu in the biomass. В© 2008 Elsevier Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, Siberian Branch, the Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Geology and Mineralogy, Siberian Branch, the Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Bondareva, L.; Sukhorukov, F.; Melgunov, M.

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New data on transuranium elements in the ecosystem of the Yenisei river floodplain / A. Bolsunovsky, A. Ermakov, A. Sobolev // Radiochimica Acta. - 2007. - Vol. 95, Is. 9. - P547-552, DOI 10.1524/ract.2007.95.9.547 . - ISSN 0033-8230
Кл.слова (ненормированные):
243,244Cm -- Blackcurrant -- Sediment and floodplain soil of the Yenisei river -- Soil-plant transfer factor (TF) -- Transuranium elements
Аннотация: The aim of the study is to investigate levels of transuranium elements in the ecosystem of the Yenisei river floodplain in the vicinity of the Mining-and-Chemical Combine of Rosatom. For the first time, the transuranium isotopes 243,244Cm have been detected in sediment, floodplain soil, and a berry shrub (Ribes nigrum - the blackcurrant) in the flood-plain of the Yenisei river. The highest level of curium isotopes registered in the sediment of the Yenisei river is 21.4 Bq/kg (dry), which is more than twice higher than maximum curium levels reported for soils sampled not far from the Chernobyl Nuclear Plant. Blackcurrant plants growing on radioactively contaminated soils contain the same transuranium elements as the soil (plutonium isotopes, americium, and curium). The highest activity concentrations of all transuranic elements have been found in ashed roots of the blackcurrant plants: 239,240Pu - 9.3 Bq/kg, 241Am - 6.9 Bq/kg, and 243,244Cm - 1.8 Bq/kg. The highest soil-plant transfer factor (TF) for 243,244Cm is determined for roots - 0.073; the TF of 243,244Cm in berries is 0.027. The TF for 239,240Pu in berries is 0.006, which is several times lower than the TF for 243,244Cm. Analysis of our results and the literature data suggests that TFs for 243,244Cm are higher than those for 239,240Pu and 241Am. В© by Oldenbourg Wissenschaftsverlag.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
SIA RADON, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Ermakov, A.; Sobolev, A.

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Accumulation and release of 241Am by a macrophyte of the Yenisei River (Elodea canadensis) / A. Bolsunovsky, T. Zotina, L. Bondareva // Journal of Environmental Radioactivity. - 2005. - Vol. 81, Is. 1. - P33-46, DOI 10.1016/j.jenvrad.2004.10.012 . - ISSN 0265-931X
Кл.слова (ненормированные):
241Am -- Accumulation -- Laboratory experiments -- Release -- Submerged plant Elodea canadensis -- Yenisei River -- Activation analysis -- Biomass -- Concentration (process) -- Effluents -- Plutonium -- Rivers -- Aquatic plants -- Elodea plant -- Macrophytes -- Radioactive contamination -- Transuranium elements -- americium 241 -- river water -- americium -- americium nitrate -- bioaccumulation -- biological uptake -- macrophyte -- pollutant source -- radioactive pollution -- river water -- submerged vegetation -- article -- biomass -- concentration (parameters) -- environmental factor -- fractionation -- laboratory -- macrophyte -- radioactivity -- river -- sampling -- adsorption -- chemistry -- Hydrocharitaceae -- methodology -- physiology -- plant -- radiation exposure -- radiation monitoring -- Russian Federation -- sediment -- soil pollutant -- time -- tissue distribution -- water pollutant -- Eastern Hemisphere -- Eurasia -- Russian Federation -- World -- Yenisei River -- Elodea canadensis -- Adsorption -- Americium -- Biomass -- Chemical Fractionation -- Geologic Sediments -- Hydrocharitaceae -- Plant Shoots -- Radiation Monitoring -- Rivers -- Russia -- Soil Pollutants, Radioactive -- Time Factors -- Tissue Distribution -- Water Pollutants, Radioactive
Аннотация: The source of radioactive contamination of the Yenisei River floodplain, including contamination with transuranic elements, is the Mining-and-Chemical Combine of the Russian Ministry of Atomic Energy, which has for many years been producing weapons-grade plutonium. Transuranic elements have been detected not only in the soil and sediment of the river but also in the biomass of aquatic plants. This work is an investigation of accumulation and release of 241Am by a submerged macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments. In 2000-2003, laboratory experiments were carried out with biomass of E. canadensis Mich. and filtered river water. The samples were collected from the Yenisei River upstream of the discharge of the Combine's radioactive effluent. The experiments showed that 241Am is accumulated by Elodea biomass: the activity concentration of 241Am can reach 3280 В± 240 Bq/g, with the concentration factor for 241Am 16 600 В± 2200 l/kg. Results of chemical fractionation have proved that in the course of 241Am accumulation by Elodea biomass, 241Am tightly bound to biomass increases from 11% to 27% of the total 241Am in the biomass. Release of 241Am from the decaying Elodea biomass has been evaluated experimentally. By the end of the experiment (lasting up to 127 days), the Elodea plants had lost up to 65% of their initial 241Am activity and the rate of 241Am release into the water environment reached 23 Bq/day. В© 2004 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Zotina, T.; Bondareva, L.

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Evidence for two abrupt warming events of SST in the last century / C. A. Varotsos [et al.] // Theoretical and Applied Climatology. - 2013. - P1-10, DOI 10.1007/s00704-013-0935-8 . - ISSN 0177-798X
Аннотация: We have recently suggested that the warming in the sea surface temperature (SST) since 1900, did not occur smoothly and slowly, but with two rapid shifts in 1925/1926 and 1987/1988, which are more obvious over the tropics and the northern midlatitudes. Apart from these shifts, most of the remaining SST variability can be explained by the El Nino Southern Oscillation and the Pacific Decadal Oscillation (PDO). Here, we provide evidence that the timing of these two SST shifts (around 60В years) corresponds well to the quasi-periodicity of many natural cycles, like that of the PDO, the global and Northern Hemisphere annual mean temperature, the Atlantic Multi-decadal Oscillation, the Inter-Tropical Convergence Zone, the Southwest US Drought data, the length of day, the air surface temperature, the Atlantic meridional overturning circulation and the change in the location of the centre of mass of the solar system. In addition, we show that there exists a strong seasonal link between SST and ENSO over the tropics and the NH midlatitudes, which becomes stronger in autumn of the Northern Hemisphere. Finally, we found that before and after each SST shift, the intrinsic properties of the SST time series obey stochastic dynamics, which is unaffected by the modulation of these two shifts. In particular, the SST fluctuations for the time period between the two SST shifts exhibit 1/f-type long-range correlations, which are frequently encountered in a large variety of natural systems. Our results have potential implications for future climate shifts and crossing tipping points due to an interaction of intrinsic climate cycles and anthropogenic greenhouse gas emissions. В© 2013 Springer-Verlag Wien.

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Держатели документа:
Climate Research Group, Division of Environmental Physics and Meteorology, Faculty of Physics, University of Athens, University Campus Bldg. Phys. V, Athens, 15784, Greece
British Antarctic Survey, Natural Environment Research Council, Cambridge, United Kingdom
Institute of Biophysics, SB RAS, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Varotsos, C.A.; Franzke, C.L.E.; Efstathiou, M.N.; Degermendzhi, A.G.

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The "quantization" of sensitivity coefficients is preserved in microbial populations heterogeneous with respect to growth rate and age / V. M. Nekrasov, A. V. Chernychev, A. G. Degermendzhy // Doklady Biological Sciences. - 2006. - Vol. 406, Is. 1-6. - P91-93, DOI 10.1134/S0012496606010261 . - ISSN 0012-4966
Кл.слова (ненормированные):
algorithm -- article -- bacterium -- biodiversity -- growth, development and aging -- population dynamics -- theoretical model -- time -- Algorithms -- Bacteria -- Biodiversity -- Models, Theoretical -- Population Dynamics -- Time Factors

Scopus
Держатели документа:
Institute of Chemical Kinetics and Combustion, Siberian Division, Russian Academy of Sciences, Institutskaya ul. 3, Novosibirsk, 630090, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nekrasov, V.M.; Chernychev, A.V.; Degermendzhy, A.G.

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Understanding the mechanisms of blooming of phytoplankton in Lake Shira, a saline lake in Siberia (the Republic of Khakasia) / A. G. Degermendzhy, R. D. Gulati // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P331-340 . - ISSN 1386-2588
Кл.слова (ненормированные):
Carbon budget -- Cyanobacteria -- Heterotrophic bacteria -- Hydrogen sulphide -- Mathematical models of stratification -- Meromictic lakes -- Microbial loop -- Stratification -- Trophic scheme -- algal bloom -- ecosystem modeling -- limiting factor -- nutrient availability -- phytoplankton -- saline lake -- trophic interaction -- Russian Federation -- algae -- Bacteria (microorganisms) -- Cyanobacteria -- Lyngbya -- Lyngbya contorta
Аннотация: The paper summarises the results of a three-year research study (European Union Grant: INTAS 97-0519) aimed at investigating the planktonic populations and trophic organization of the Lake Shira ecosystem - a saline lake in Khakasia, Siberia. The lake exhibits a stable summer-autumn stratification of the chemical-biological components. The mechanisms responsible for the 'blooming' of phytoplankton in the deeper layers were investigated in greater detail, using data from both field and laboratory experiments. The spectra of nutrition were examined to estimate the relationships between the specific growth rates of the hydrobionts and the influence of the limiting factors: light, nutrients. The observed heterotrophic capability of a metalimnetic phytoplankton population might help explain the development in the deeper waters of Lyngbya contorta. The scheme of trophic interactions was put up, based on the assessment of the carbon pools and carbon flows in the pelagic zone of the lake. A mathematical model of the vertical structure of the lake's plankton populations was constructed, using the ecosystem description and data of vertical turbulent diffusion. The role of light and nutrient limitations and grazing mortality in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with the field observations, e.g. for the different patterns of the peaks, and for the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. Based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been theoretically discriminated for the first time. It has also been ascertained that irrespective of the depth the allochthonous substances limiting bacterial growth, the bacteria are uniformly distributed over depth and can serve as an indicator of the presence of limitation (the effect of autostabilisation in space). Of indisputable interest to limnology are the specific methods developed for understanding the functioning of Lake Shira ecosystem. These include the autostabilisation of the limiting factors, the on-the-spot fluorescent method of determining the three classes of microalgae, the algal mixotrophy and the planktonic population interactions and feedbacks, and development of a more sensitive, bioluminescent method for mapping the nonhomogeneities. Owing to a balanced combination of classical approaches (field observations, in situ data on production-decomposition) and the more recent ones (satellite monitoring, biophysical methods of estimating interactions of populations, mathematical models based on the field and experimental data), many of the structural-function relationships in the ecosystem can now be explained, and the models can provide 'mutual control and mutual agreement' between the data collected using different approaches.

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Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Gulati, R.D.

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10.

Formation of the vertical heterogeneity in the Lake Shira ecosystem: The biological mechanisms and mathematical model / A. G. Degermendzhy [et al.] // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P271-297, DOI 10.1023/A:1015621508971 . - ISSN 1386-2588
Кл.слова (ненормированные):
Hydrogen sulphide -- Phytoplankton -- Stratification control -- Sulphate-reducing bacteria -- Sulphur cycle -- Vertical model -- biological production -- community structure -- ecosystem modeling -- nutrient cycling -- plankton -- saline lake -- seasonal variation -- vertical distribution -- Russian Federation -- algae -- Arctodiaptomus -- Bacteria (microorganisms) -- Calanoida -- Chlorophyta -- Copepoda -- Crustacea -- Cyanobacteria -- Dictyosphaerium -- Lyngbya -- Lyngbya contorta
Аннотация: Data on the seasonal changes in vertical heterogeneity of the physical-chemical and biological parameters of the thermally stratified Shira Lake ecosystem (Khakasia, Siberia) in 1996-2000 have been analyzed. The interaction mechanisms involving: (1) The plankton populations in aerobic and anaerobic zones, involving the cycling of carbon and sulphur, (2) the primary production limitation (by light and phosphorus) and inhibition (by light), and (3) the kinetic characteristics of plankton populations have been elucidated. A mathematical model of the vertical structure of the lake's plankton populations, based on the ecosystem description and on vertical turbulent diffusion of the matter, has been constructed. The green alga Dictyosphaerium tetrachotomum (Chlorophyta) and the cyanobacterium Lyngbya contorta (Cyanophyta), which dominated the phytoplankton biomass, were taken as oxygen producers. Arctodiaptomus salinus (a calanoid copepod) has been assumed as the main grazer in Shira Lake as it dominated the zooplankton biomass. Four groups of microorganisms involved in the sulphur cycle formation have been distinguished: sulphur, sulphur purple, sulphur green and SRB. H2S is oxidized to sulphate (only the green sulphur bacteria oxidize it to sulphur), and sulphate is reduced to H2S, forming neither sulphur nor its water-soluble compounds. The role of grazing, light and nutrient limitation, in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. When the model takes into account both light limitation and nutrient limitation of algal growth by P and consumption of algae by crustaceans: (a) in the scenario where the P is formed only by the cycling and decomposition of autochthonous organic matter, both the green algae and cyanobacteria are eliminated; (b) in the scenario involving an additional P flux in the deep water layers the peak of the cyanobacteria is at a depth of 10 m, and its amplitude is close to the one observed in the lake. The position of the peak remains stable owing to the 'double' limitation mechanism: light 'from above' and P 'from below'. Another mechanism responsible for the deep position of the peak of cyanobacteria was analyzed mathematically based on the model involving the experimentally proven assumption of the growth inhibition by light in the epilimnion and the light limitation in the hypolimnion. The main result is: the peak is positioned stable at its depth and does not change with time. The analytical and numerical calculations made for this positioning mechanism yielded the formulae relating the depth of the maximum of algal biomass, the 'width' of the peak base and the peak amplitude and a number of parameters (algae elimination, turbulent diffusion coefficient, sedimentation rate, light extinction coefficient and light intensity). The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with field observations, e.g. for the different patterns for the peaks, and the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. For the first time, theoretically, based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been discriminated. The theoretical limit for the depth up to which the hydrogen-sulphide zone can ascend under the impact of allochthonous organic loading, has been determined.

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Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Belolipetsky, V.M.; Zotina, T.A.; Gulati, R.D.

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11.

Cell growth and accumulation of polyhydroxyalkanoates from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646 / T. G. Volova [et al.] // Bioresource Technology. - 2013. - Vol. 146. - P215-222, DOI 10.1016/j.biortech.2013.07.070 . - ISSN 0960-8524
Кл.слова (ненормированные):
Autotrophic synthesis -- Cupriavidus eutrophus -- Polyhydroxyalkanoates
Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h-1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h-1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, ?-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. В© 2013 Elsevier Ltd.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
L.V. Kirenckii Institute of Physics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kiselev, E.G.; Shishatskaya, E.I.; Zhila, N.O.; Boyandin, A.N.; Syrvacheva, D.A.; Vinogradova, O.N.; Kalacheva, G.S.; Vasiliev, A.D.; Peterson, I.V.

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12.

An in vivo study of pha matrices of different chemical composition: Tissue reaction and biodegradation / E. I. Shishatskaya [et al.] // Cellular Transplantation and Tissue Engineering. - 2012. - Vol. 7, Is. 1. - P73-80 . - ISSN 1815-445X
Кл.слова (ненормированные):
Degradable polymer matrices -- Degradation -- PHAs -- Polyhydroxyalkanoates -- Subcutaneous implantation -- Tissue reaction
Аннотация: The study addresses consequences of subcutaneous implantation of film matrices prepared from different PHAs to laboratory animals. No negative effects of subcutaneous implantation of PHA matrices on physiological and biochemical characteristics of the animals were determined. Independently of the matrices composition and duration of the contact with the internal environment of the organism we did not observe any deviations in the behavior of animals, their growth and development, as well as blood functions. Response of the tissues to PHA matrices was comparable with the response to polylactide, but substantially less expressed at the earlier time periods after implantation. Tissues response to implantation of PHA of all types is characterized by short-term (up to 2 weeks) post-traumatic inflammation with formation of fibrous capsules by 30th-60th days with the thickness less than 100 microns, which get thinner down to 40-60 microns by 180th day as the result of involution. No differences in response of tissues and the whole organism were observed for the matrices produced from the homopolymer of 3-hydroxybutyric acid (P3HB), copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate acids (P3HB/3HH). Macrophages and foreign-body giant cells actively participate in the response of the tissues to PHAs. In the studied conditions matrices from the copolymers containing 3-hydroxyhexanoate and 4 hydroxybutyrate were determined as more actively degraded PHA. The next less degraded matrices were matrices from the copolymer of P3HB/3HV and the most resistant were P3HB matrices. The slower degradation of PHA matrices was accompanied by delayed development of giantcells response. The studied PHA matrices can be placed in the following range by their degradation: P3HB/3HH - P3HB/4HB - P3HB/HV - P3HB.

Scopus
Держатели документа:
Institute of Modern Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Massachusetts Institute of Technology, Cambridge, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Nikolaeva, E.D.; Goreva, A.V.; Brigham, C.J.; Volova, T.G.; Sinskey, A.J.

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13.

Biodegradation of polyhydroxyalkanoates (PHAs) in the South China Sea and identification of PHA-degrading bacteria / T. G. Volova [et al.] // Microbiology. - 2011. - Vol. 80, Is. 2. - P252-260, DOI 10.1134/S0026261711020184 . - ISSN 0026-2617
Кл.слова (ненормированные):
biodegradation in marine environments -- microbial PHA degraders -- PHA -- polyhydroxyalkanoates -- Bacillus (bacterium) -- Bacillus sp. -- Bacteria (microorganisms) -- Enterobacter -- Enterobacter cloacae -- Gracilibacillus -- Prospect Hill virus
Аннотация: The biodegradation patterns of two types of PHA, a 3-hydroxybutyrate (3-PHB) polymer and a 3-hydroxybutyrate and 3-hydroxyvalerate (3-PHB/3-PHV) copolymer, were studied in tropical marine environments (Dam Bay, South China Sea, Nha Trang, Vietnam). No reliable differences in the degradation of 3-PHB and 3-PHB/3-PHV were revealed. It was shown that the degradation process depended mainly on the shape of a polymer product and its production method: the degradation of polymer films was found to be more active than that of molded solids. A decrease in the molecular mass of both types of PHA was detected in the course of the degradation of PHA samples. However, the degree of PHA crystallinity did not change; that is, the levels of degradation of both the amorphous and crystalline phases of PHA were almost the same. Among microbial PHA degraders, three bacterial strains, Bacillus sp. IBP-V002, Enterobacter cloacae sp. IBP-V001, and Gracilibacillus sp. IBP-V003, were identified based on the results of morphological, biochemical, and molecular phylogenetic analyses. The ability of the representatives of the genera Gracilibacillus and Enterobacter to degrade PHA was revealed for the first time. В© 2011 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russian Federation
Marine Department, Joint Russian-Vietnamese Tropical Research and Test Center, Nha Trang, Viet Nam : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Boyandin, A.N.; Vasil'ev, A.D.; Karpov, V.A.; Kozhevnikov, I.V.; Prudnikova, S.V.; Rudnev, V.P.; Xuan, B.B.; Dung, V.V.; Gitel'zon, I.I.

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14.

Biocompatibility of polyhydroxybutyrate microspheres: In vitro and in vivo evaluation / E. I. Shishatskaya [et al.] // Journal of Materials Science: Materials in Medicine. - 2008. - Vol. 19, Is. 6. - P2493-2502, DOI 10.1007/s10856-007-3345-6 . - ISSN 0957-4530
Кл.слова (ненормированные):
Biocompatibility -- Drug delivery -- Fibroblasts -- Ion implantation -- Microspheres -- Polymer matrix -- Fibroblast cells -- Hydroxybutyric acid -- Intramuscular implantation -- Polyhydroxybutyrate -- Organic polymers -- microsphere -- poly(3 hydroxybutyric acid) -- polymer -- animal cell -- animal experiment -- animal tissue -- article -- biocompatibility -- cell infiltration -- controlled study -- giant cell -- implantation -- in vitro study -- in vivo study -- inflammation -- macrophage -- mouse -- nonhuman -- priority journal -- rat -- 3-Hydroxybutyric Acid -- Animals -- Biocompatible Materials -- Cell Survival -- Inflammation -- Materials Testing -- Mice -- Microspheres -- Necrosis -- NIH 3T3 Cells -- Polymers -- Rats -- Rats, Wistar -- Tetrazolium Salts -- Thiazoles -- Time Factors -- Rattus norvegicus
Аннотация: Microspheres have been prepared from the resorbable linear polyester of ?-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection. В© 2008 Springer Science+Business Media, LLC.

Scopus
Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Laboratory of Bacterial Bioluminescence, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svo Bodnyi Av. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Voinova, O.N.; Goreva, A.V.; Mogilnaya, O.A.; Volova, T.G.

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15.

Biosynthesis of multicomponent polyhydroxyalkanoates by Wautersia eutropha / T. G. Volova [et al.] // Microbiology. - 2007. - Vol. 76, Is. 6. - P704-711, DOI 10.1134/S0026261707060082 . - ISSN 0026-2617
Кл.слова (ненормированные):
Autotrophic and mixotrophic growth -- Multicomponent polyhydroxyalkanoates -- Wautersia eutropha -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: The effect of carbon supply on polyhydroxyalkanoate (PHA) synthesis by bacteria Wautersia eutropha was studied. Synthesis of multicomponent PHA composed of short-and long-chain monomers (C4-C8) by two natural strains (H16 and B5786) under mixotrophic conditions (CO2 + alkanoic acids as cosubstrates) was demonstrated for the first time. The PHA composition was shown to be dependent on the cosubstrate type. In the presence of odd fatty acids, four-and five-component polymers were synthesized; hydroxybutyrate, hydroxyvalerate, and hydroxyheptanoate were the major monomers, while hydroxyhexanoate and hydroxyoctanoate were minor. In the presence of even fatty acids, PHA contained not only the corresponding molecules (hydroxyhexanoate and hydroxyoctanoate), but also hydroxyvalerate; synthesis of four-component PHA which contain mainly hydroxybutyrate and hydroxyhexanoate (up to 18 mol %) is therefore possible. A series of four-and five-component PHA was synthesized and their physicochemical characteristics were determined. В© 2007 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation
Institute of Molecular Biology and Biotechnology, Munster, Germany : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Kozhevnikov, I.V.; Steinbuchel, A.

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16.

Luminous bacteria as potential producers of resorbed polyhydroxyalkanoate polyesters / A. N. Boyandin [et al.] // Doklady Biochemistry and Biophysics. - 2007. - Vol. 416, Is. 1-6. - P248-251, DOI 10.1134/S1607672907050067 . - ISSN 1607-6729
Кл.слова (ненормированные):
photoprotein -- polyester -- polyhydroxyalkanoic acid -- article -- bacterium -- chemistry -- chemoluminescence -- classification -- genetic procedures -- genetics -- metabolism -- Photobacterium -- phylogeny -- time -- Bacteria -- Biosensing Techniques -- Chemiluminescent Measurements -- Luminescent Proteins -- Photobacterium -- Phylogeny -- Polyesters -- Polyhydroxyalkanoates -- Time Factors -- Bacteria (microorganisms)

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Kalacheva, G.S.; Rodicheva, E.K.; Volova, T.G.

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17.

Effect of nitrogen limitation on the growth and lipid composition of the green alga Botryococcus braunii Kutz IPPAS H-252 / N. O. Zhila, G. S. Kalacheva, T. G. Volova // Russian Journal of Plant Physiology. - 2005. - Vol. 52, Is. 3. - P311-319, DOI 10.1007/s11183-005-0047-0 . - ISSN 1021-4437
Кл.слова (ненормированные):
Botryococcus -- Lipids -- Nitrogen limitation -- algae -- Botryococcus -- Botryococcus braunii -- Chlorophyta
Аннотация: The effect of nitrogen limitation in a medium on the composition of intracellular lipids in the alga Botryococcus braunii Kutz IPPAS H-252 in the course of culture development was investigated. Under the conditions of nitrogen limitation, the alga under investigation accumulated lipids as triacylglycerols, and this process was accompanied by substantial changes in the total fatty acid (FA) composition, which were manifested in a decrease in trienoic acids (from 52.8-57.2 to 19.5-24.7% of total FAs) and an increase in the content of oleic (from 1.1-1.2 to 17.1-24.4%) and saturated (from 23.7-26.0 to 32.9-46.1%) acids. In the control culture, the directionality of FA redistribution was less marked, and these changes were noticed at the later stages of culture development. Under nitrogen limitation, marked changes in the FA composition of polar lipids occurred by the 13th day, and they were characterized by an increase in the content of saturated acids (up to 76.8%) and a dramatic decrease in the content of all polyenoic acids (up to 6.8%). The changes in the FA composition of triacylglycerols were noticed as early as by the 7th day; these changes consisted in an increase in the content of oleic acid, and its high content (28.4-38.4%) was maintained up to the end of culturing. In the control culture, triacylglycerols with a high content of oleic acid were found by the 13th day, although, by this time, the content of total lipids and triacylglycerols did not change. В© 2005 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhila, N.O.; Kalacheva, G.S.; Volova, T.G.

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18.

Degradation of P(3HB) and P(3HB-co-3HV) in biological media / E. I. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - P643-657, DOI 10.1163/1568562053783678 . - ISSN 0920-5063
Кл.слова (ненормированные):
Degradation rate -- Fiber properties -- Morphology -- Poly(hydroxybutyrate-co-hydroxyvalerate) (P(3HB-co-3HV)) -- Polyhydroxyalkanoates(PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Copolymers -- Degradation -- Differential scanning calorimetry -- Enzymes -- Morphology -- Scanning electron microscopy -- Tensile strength -- Tissue -- Transmission electron microscopy -- Degradation rate -- Fiber properties -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Biopolymers -- buffer -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxybutyrate hydroxyvalerate copolymer -- unclassified drug -- animal experiment -- animal model -- animal tissue -- article -- biodegradation -- controlled study -- crystal structure -- fiber -- giant cell -- in vitro study -- in vivo study -- macrophage -- morphology -- nonhuman -- pH -- priority journal -- rat -- structure analysis -- tensile strength -- tissue water -- weight reduction -- Animals -- Biodegradation, Environmental -- Buffers -- Humans -- Hydrogen-Ion Concentration -- Hydroxybutyrates -- Macrophages -- Microscopy, Electron, Scanning -- Microscopy, Electron, Transmission -- Muscle, Skeletal -- Polyesters -- Rats -- Rats, Wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 600326, Russian Federation
Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G3, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gordeev, S.A.; Puzyr, A.P.

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19.

Degradation of bioplastic in environment / T. G. Volova [и др.] // Doklady Akademii Nauk. - 2004. - Vol. 397, Is. 5. - С. 708-710 . - ISSN 0869-5652
Кл.слова (ненормированные):
Bacteria -- Ecology -- Environmental testing -- Plastics -- Weathering -- Time dependence -- Biomaterials
Аннотация: For the first time degradation kinetics is investigated for polyhydroxyalkanoates (PHA) in the native fresh pond. Its substantial variability in dependence on ecological conditions is shown, new bacteria species (clones) capable for PHA utilization in fresh-water ecosystem are identified.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Golyshev, M.I.; Trusova, M.Yu.; Zhila, N.O.; Kartushinskaya, M.V.

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20.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786 / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 2. - P170-177, DOI 10.1023/B:ABIM.0000018921.04863.d5 . - ISSN 0003-6838
Кл.слова (ненормированные):
acetyl coenzyme A acyltransferase -- bacterial enzyme -- carbon -- carbon dioxide -- fructose -- hydrogen -- hydroxybutyrate dehydrogenase -- oxidoreductase -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- synthetase -- article -- bacterial metabolism -- carbon source -- catalysis -- controlled study -- degradation -- depolymerization -- enzyme activity -- enzyme analysis -- molecular dynamics -- nonhuman -- protein function -- Ralstonia eutropha -- recording -- statistical significance -- synthesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (?-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of ?-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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