Библиотека института биофизики СО РАН

Главная

Базы данных

Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

Каталог диссертаций ИБФ СО РАН

Труды сотрудников ИБФ СО РАН

Область поиска

в найденном

Найдено в других БД:

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (3)

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>K=toxicity<.>)

Общее количество найденных документов : 62
Показаны документы с 1 по 20

1-20 21-40 41-60 61-62

Assessment of the rate of accumulation of the transuranium element americium-241 by the aquatic plant Elodea canadensis / A. Ya. Bolsunovskii [et al.] // Doklady Biological Sciences. - 2004. - Vol. 399, Is. 1-6. - P467-469, DOI 10.1007/s10630-005-0014-0 . - ISSN 0012-4966
Кл.слова (ненормированные):
americium -- radioisotope -- water -- article -- biomass -- drug effect -- environmental monitoring -- Hydrocharitaceae -- methodology -- pH -- plant -- plant root -- radiation exposure -- radiochemistry -- time -- toxicity testing -- water pollutant -- Americium -- Biomass -- Environmental Monitoring -- Hydrocharitaceae -- Hydrogen-Ion Concentration -- Plant Roots -- Plant Shoots -- Plants -- Radiochemistry -- Radioisotopes -- Time Factors -- Toxicity Tests -- Water -- Water Pollutants, Radioactive -- Elodea canadensis

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Ya.; Zotina, T.A.; Bondareva, L.G.; Degermendzhi, A.G.

Найти похожие

The effect of radionuclide and heavy metal contamination of the Yenisei River on cytogenetics of aquatic plant Elodea canadensis / A. Bolsunovsky [et al.] // Radioprotection. - 2009. - Vol. 44, Is. 5. - P83-88, DOI 10.1051/radiopro/20095021 . - ISSN 0033-8451
Аннотация: The study was done to determine concentrations of radionuclides and heavy metals and to evaluate the frequency of chromosomal aberrations in samples of Elodea canadensis, a submerged plant, collected in different parts of the Yenisei River. Samples were collected in the area subjected to radioactive impact of theMining-and-Chemical Combine (MCC) at Zheleznogorsk and in the control areas, upstream of the MCC. The investigations showed that Elodea biomass in the area affected by MCC operation contained a long inventory of artificial radionuclides typical of the MCC discharges. Upstream of the MCC, in the control sampling areas, the sediments and the Elodea biomass contained only one artificial radionuclide - 137Cs. Thus, the exposure doses to Elodea shoots and roots upstream of the MCC are small (not more than 8 ?Gy/d) and the main contribution to them is made by natural radionuclides. At the MCC discharge site (the village of Atamanovo) and downstream of it, the total dose rate increases almost an order of magnitude, reaching its maximal values - 72 ?Gy/d for Elodea shoots and 58 ? Gy/d for roots. Cytogenetic investigations of Elodea roots showed that at the MCC discharge site (the village of Atamanovo) and downstream of it the occurrence of chromosomal aberrations in ana-telophase and metaphase cells of Elodea was considerably higher than in the control area. It is highly probable that this simultaneous dramatic increase in the total exposure rate and the occurrence of chromosomal aberrations in Elodea is associated with the radiation factor. It is suggested that Elodea is affected not only by the radiation factor but also by the chemical factor - toxicity of heavy metals. В© 2009 EDP Sciences.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodnyi Ave., 660041 Krasnoyarsk, Russian Federation
Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Muratova, E.; Sukovaty, A.; Kornilova, M.

Найти похожие

The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems / V. A. Kratasyuk [et al.] // Chemosphere. - 2001. - Vol. 42, Is. 8. - P909-915, DOI 10.1016/S0045-6535(00)00177-6 . - ISSN 0045-6535
Кл.слова (ненормированные):
Alcohol dehydrogenase -- Bacterial luciferase -- Bioluminescence -- Blooming -- Pollution -- Trypsin -- Water toxicity -- alcohol dehydrogenase -- benzoquinone -- luciferase -- trypsin -- aquatic ecosystem -- bioluminescence -- water quality -- article -- bacterium culture -- bioluminescence -- blue green alga -- ecosystem -- pond -- seasonal variation -- water pollution -- water quality -- Benzoquinones -- Biological Assay -- Cyanobacteria -- Ecosystem -- Environmental Monitoring -- Eutrophication -- FMN Reductase -- Indicators and Reagents -- Luminescent Measurements -- NADH, NADPH Oxidoreductases -- Water Pollutants -- Russian Federation -- algae -- Bacteria (microorganisms) -- Chlorophyta -- Cyanobacteria -- uncultured cyanobacterium
Аннотация: A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductase-luciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. В© 2001 Elsevier Science Ltd.

Scopus
Держатели документа:
Krasnoyarsk State University, pr. Svobodnii 79, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State Agricultural University, Mira av., 88, Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Esimbekova, E.N.; Gladyshev, M.I.; Khromichek, E.B.; Kuznetsov, A.M.; Ivanova, E.A.

Найти похожие

Assessment of composition and toxicity for plants of gases produced during physicochemical processing of human exometabolites as applied to biotechnical life support systems / A. A. Tikhomirov [et al.] // Doklady Biochemistry and Biophysics. - 2011. - Vol. 441, Is. 1. - P252-254, DOI 10.1134/S1607672911060032 . - ISSN 1607-6729
Кл.слова (ненормированные):
ammonia -- carbon dioxide -- nitrogen oxide -- oxygen -- article -- biomass -- bioremediation -- drug effect -- gas -- growth, development and aging -- human -- instrumentation -- methodology -- microclimate -- plant -- waste management -- Ammonia -- Biodegradation, Environmental -- Biomass -- Carbon Dioxide -- Ecological Systems, Closed -- Gases -- Humans -- Life Support Systems -- Nitrogen Oxides -- Oxygen -- Plants -- Waste Management

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Boreskov Institute of Catalysis, Omsk Branch, Siberian Branch, Russian Academy of Sciences, ul. Neftezavodskaya 54, Omsk 644053, Russian Federation
Siberian Federal University, Svobodnyi pr. 41, Krasnoyarsk 660079, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Kudenko, Y.A.; Degermendzhi, A.G.; Trifonov, S.V.; Sutormina, E.F.; Ivanova, Y.A.

Найти похожие

The influence of microbial associations on germination of wheat seeds and growth of seedlings under impact of zinc salts / L. A. Somova, N. S. Pechurkin // Advances in Space Research. - 2009. - Vol. 43, Is. 8. - P1224-1228, DOI 10.1016/j.asr.2008.12.008 . - ISSN 0273-1177
Кл.слова (ненормированные):
Heavy metals -- Life support system -- Microbial associations -- Microorganisms -- Wheat seeds -- Concentration (process) -- Heavy metals -- Metals -- Microorganisms -- Recycling -- Salts -- Seed -- Zinc -- Adverse effects -- Biological recycling -- Growth and development -- Heavy metal salts -- Higher plants -- Investigate effects -- Life support system -- Long-term missions -- Maximum permissible concentrations -- Microbial associations -- Pathogenic organisms -- Plantlets -- Recycling systems -- Root systems -- Wheat seeds -- Zinc salts -- Grain (agricultural product)
Аннотация: The life support systems (LSS) for long-term missions are to use cycling-recycling systems, including biological recycling. Higher plants are the traditional regenerator of air and producer of food. They should be used in many successive generations of their reproduction in LSS. Studies of influence of microbial associations on germination of wheat seeds and on growth of seedlings under impact of heavy metals are necessary because of migration of heavy metals in LSS. Microbial associations are able to stimulate growth of plants, to protect them from pathogenic organisms and from toxicity of heavy metal salts. The goal of this work was to investigate effect of microbial associations on the germination of wheat seeds and on the growth of seedlings under impact of different concentrations of ZnSO4. The results of investigations showed that:(1)Zinc salt had an adverse effect on germination of wheat seeds, beginning with concentrations of 8 MPC (Maximum Permissible Concentration) and higher.(2)Microbial associations (concentrations -104 to 107 cells/ml) were able to decrease (partly or completely) the adverse effect of ZnSO4 on germination of wheat seeds.(3)Concentrations (104-107 cells/ml) of microbial associations were able to decrease partly the adverse effect of zinc salts (intervals: from 1 to 32 MPS{cyrillic}) on the growth and development of wheat plantlets during heterotrophic phase.(4)The root system of plants was more sensitive to the adverse effect of ZnSO4 than shoots of plants. В© 2008 COSPAR.

Scopus
Держатели документа:
Institute of Biophysics SB, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.

Найти похожие

Biotesting of Effluent and River Water by Lyophilized Luminous Bacteria Biotest / A. M. Kuznetsov, E. K. Rodicheva, S. E. Medvedeva // Field Analytical Chemistry and Technology. - 1998. - Vol. 2, Is. 5. - P267-275 . - ISSN 1086-900X
Кл.слова (ненормированные):
Bioluminescence -- Effluent -- Luminous bacteria biotest -- Toxicity -- Water quality
Аннотация: Waters of the Yenisei River, certain rivers and lakes of the Altai Territory, and effluents of some industrial factories in Krasnoyarsk were studied by luminous bacteria biotest Microbiosensor B17 677F. The lyophilized luminous bacteria Photobacterium phosphoreum from the IBSO collection were used to design this biotest. The bioluminescent test is based on bioluminescence quenching resulting from the action of water samples on luminous bacteria. The test results indicated locations and zones of impaired water quality. The heaviest pollution of water in the Yenisei River was recorded in the zones 0-116 km downstream from Krasnoyarsk (Krasnoyarsk and satellite towns). The effluents of most factories were found to be toxic. Underground and surface waters of some areas of the Altai Territory had different toxicity levels; there were deviations from the norm in most water samples taken from the different lakes and rivers. The data from this study show that the luminous bacteria biotest is simple and convenient, and that the results obtained are within acceptable levels of accuracy for the evaluation of the toxicity of effluent and river water. It takes no more than 30 min to do the biotest. It can be used in ecological monitoring like the Microtox toxicity test. В© 1998 John Wiley & Sons, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kuznetsov, A.M.; Rodicheva, E.K.; Medvedeva, S.E.

Найти похожие

Analysis of river water by bioluminescent biotests / A. M. Kuznetsov, E. K. Rodicheva, S. E. Medvedeva // Luminescence. - 1999. - Vol. 14, Is. 5. - P263-265 . - ISSN 1522-7235
Кл.слова (ненормированные):
Bioluminescent bioassay -- Pollution -- Water quality -- fresh water -- article -- bioassay -- Escherichia coli -- freeze drying -- genetic procedures -- luminescence -- methodology -- Photobacterium -- Russian Federation -- sensitivity and specificity -- water pollutant -- Biological Assay -- Biosensing Techniques -- Escherichia coli -- Freeze Drying -- Fresh Water -- Luminescence -- Photobacterium -- Russia -- Sensitivity and Specificity -- Water Pollutants, Chemical
Аннотация: The bacterial bioluminescence has high sensitivity to the action of various inhibitors of biological activity. The lyophilized luminous bacteria Photobacterium phosphoreum (Microbiosensor B17 677F) and luminous strain Escherichia coli (Microbiosensor EC) from the Culture Collection IBSO were used to create bioluminescent biotests. They have been applied in ecological monitoring to determine the overall toxicity of the Yenisei and Angara Rivers and some water sources of Altai Territory. As a rule the heaviest pollution of water in studied rivers was registered near cities and settlements. The luminous bacteria biotests are simple and convenient in work, standardized and quantitative, have rapid response to actions of different substances and high sensitivity to environmental pollutants. It takes less than 30 min to do the biotest (the other biotests take 48-96 h). Copyright В© 1999 John Wiley & Sons, Ltd.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kuznetsov, A.M.; Rodicheva, E.K.; Medvedeva, S.E.

Найти похожие

Bioluminescent method in studying the complex effect of sewage components / D. I. Stom [et al.] // Archives of Environmental Contamination and Toxicology. - 1992. - Vol. 22, Is. 2. - P203-208 . - ISSN 0090-4341
Кл.слова (ненормированные):
heavy metal -- phenol derivative -- quinone derivative -- article -- bacterium -- bioluminescence -- cell membrane -- nonhuman -- priority journal -- sludge -- ultrastructure -- Benzoquinones -- Catechin -- Hydroquinones -- Luminescent Measurements -- Metals -- Phenol -- Phenols -- Photobacterium -- Sewage -- Vibrio -- Water Pollutants, Chemical -- Bacteria (microorganisms)
Аннотация: The inhibition of bacterial luminescence has been used in testing industrial enterprises sewage. The toxicity of the sewage is less than the total toxicity of separate components due to neutralization of quinone products of polyphenol oxidation in the reactions with the other phenol components of sewage. Toxicity increase is due to their influence on the cell membrane. Studies of cell ultrastructure confirm this fact. The studied mechanism of the complex effect allowed a more accurate forecast of the ecological situation during the discharge of phenol compounds and metals. It also showed the necessity of taking into account the complex effect of sewage components on contaminant discharge into water reservoirs.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Stom, D.I.; Geel, T.A.; Balayan, A.E.; Shachova, G.I.; Kuznetsov, A.M.; Medvedeva, S.E.

Найти похожие

BIOLUMINESCENT ANALYSIS - THE ACTION OF TOXICANTS - PHYSICAL-CHEMICAL REGULARITIES OF THE TOXICANTS EFFECTS [Text] / N. S. KUDRYASHEVA, V. A. KRATASYUK, P. I. BELOBROV // Anal. Lett. - 1994. - Vol. 27, Is. 15. - P2931-2947. - Cited References: 13 . - 17. - ISSN 0003-2719

РУБ Chemistry, Analytical

Кл.слова (ненормированные):
BACTERIAL LUCIFERASE BIOTEST -- FOREIGN COMPOUNDS -- ENERGY OF ELECTRON EXCITED STATES LEVEL -- REDOX POTENTIAL -- REDUCING OF BIOLUMINESCENT INTENSITY -- INDUCTION PERIOD -- TIME OF MAXIMUM LIGHT INTENSITY
Аннотация: The physical-chemical regularities of aromatic compounds' effects in luciferase to toxicity biotesting have been studied, The structures and physical-chemical characteristics of the toxicants and of the bioluminescent emitter were taken into account. The inhibition constants of bioluminescence intensity (I) were calculated and interpreted from the viewpoint of the energy (electron) transfer processes. The induction period (P) and the increase of the rime of the maximum light intensity (t(M)) which take place in the quinones presence, have been shown to deal with hydrogen transfer processes. The values of I, P and t(M) have been shown to be connected with a size of the quinones' aromatic and aliphatic parts, P- and t(M)-dependencies on quinone's redox potential have been demonstrated.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
KUDRYASHEVA, N.S.; KRATASYUK, V.A.; BELOBROV, P.I.

Найти похожие

10.

Effect of low-level alpha-radiation on bioluminescent assay systems of various complexity [Text] / T. V. Rozhko [et al.] // Photochem. Photobiol. Sci. - 2007. - Vol. 6, Is. 1. - P67-70, DOI 10.1039/b614162p. - Cited References: 52 . - 4. - ISSN 1474-905X

РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical
Рубрики:
LOW-DOSE RADIATION
   IRRADIATION
   TOXICITY
   QUINONES
   HORMESIS
   PHENOLS
Аннотация: This study addresses the effects of low-level alpha-radiation on bioluminescent assay systems of different levels of organization: in vivo and in vitro. Three bioluminescent assay systems are used: intact bacteria, lyophilized bacteria, and bioluminescent system of coupled enzyme reactions. Solutions of Am-241(NO3)(3) are used as a source of alpha-radiation. It has been shown that activation processes predominate in all the three bioluminescent assay systems subjected to short-term exposure (20-55 h) and inhibition processes in the systems subjected to longer-term exposure to radiation. It has been found that these effects are caused by the radiation component of Am-241(3+) impact. The intensity of the Am-241(3+) effect on the bioluminescent assay systems has been shown to depend on the Am-241(3+) concentration, level of organization and integrity of the bioluminescent assay system. The bioluminescent assay systems in vivo have been found to be highly sensitive to Am-241(3+) (up to 10(-17) M).

Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rozhko, T.V.; Kudryasheva, N.S.; Kuznetsov, A.M.; Vydryakova, G.A.; Bondareva, L.G.; Bolsunovsky, A.Y.

Найти похожие

11.

The adsorption of aflatoxin B1 by detonation-synthesis nanodiamonds [Text] / A. P. Puzyr [et al.] // Dokl. Biochem. Biophys. - 2007. - Vol. 417, Is. 1. - P299-301, DOI 10.1134/S1607672907060026. - Cited References: 15 . - ISSN 1607-6729

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
TOXICITY

Держатели документа:
[Puzyr, A. P.
Purtov, K. V.
Bondar, V. S.] Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
[Luo, M.
Brenner, D. W.] N Carolina State Univ, Dept Mat Sci & Engn, Raleigh, NC 27695 USA
[Shenderova, O. A.] Int Technol Ctr, Raleigh, NC USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr, A.P.; Purtov, K.V.; Shenderova, O.A.; Luo, M...; Brenner, D.W.; Bondar, V.S.

Найти похожие

12.

    Optical correction of the results of bioassays based on bacterial bioluminescence in vitro applied to nanoparticles [Text] / A. A. Lisitsa, E. V. Nemtseva, V. A. Kratasyuk // Luminescence. - 2014. - Vol. 29. - P74-75. - Cited References: 5 . - ISSN 1522-7235. - ISSN 1522-7243
Рубрики:
TOXICITY
   WATER

WOS
Держатели документа:
[Lisitsa, Albert A.
Nemtseva, Elena V.
Kratasyuk, Valentina A.] Siberian Fed Univ, Krasnoyarsk, Russia
[Nemtseva, Elena V.
Kratasyuk, Valentina A.] Inst Biophys SB RAS, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lisitsa, A.A.; Nemtseva, E.V.; Kratasyuk, V.A.

Найти похожие

13.

Biological module of bioluminescent biosensor for environmental toxicity assay [Text] / V. . Lonshakova-Mukina, E. . Esimbekova, V. . Kratasyuk // Luminescence. - 2014. - Vol. 29. - P72-72. - Cited References: 4 . - ISSN 1522-7235. - ISSN 1522-7243

WOS
Держатели документа:
[Lonshakova-Mukina, Victoria
Esimbekova, Elena
Kratasyuk, Valentina] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk, Russia
[Esimbekova, Elena
Kratasyuk, Valentina] Inst Biophys SB RAS, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lonshakova-Mukina, V...; Esimbekova, E...; Kratasyuk, V...

Найти похожие

14.

    Bioluminescence as a tool for study Mechanisms of Radiation Hormesis and Radiation Toxicity [Text] / N. . Kudryasheva [et al.] // Luminescence. - 2014. - Vol. 29. - P26-27. - Cited References: 6 . - ISSN 1522-7235. - ISSN 1522-7243
Рубрики:
SYSTEMS
   AM-241

WOS
Держатели документа:
[Kudryasheva, Nadezhda
Selivanova, Maria] RAS, Inst Biophys Sb, Krasnoyarsk, Russia
[Kudryasheva, Nadezhda
Petrova, Alena
Rozhko, Tatiana] Siberian Fed Univ, Krasnoyarsk, Russia
[Tugarova, Anna
Kamnev, Alexander] RAS, Inst Biochem & Physiol Plants & Microorganisms, Saratov, Russia
[Devyatlovskaya, Anna] State Technol Univ Lb, Lesosibirsk, Krasnoyarsk Reg, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N...; Selivanova, M...; Petrova, A...; Rozhko, T...; Tugarova, A...; Kamnev, A...; Devyatlovskaya, A...

Найти похожие

15.

In vitro and transdermal penetration of PHBV micro/nanoparticles [Text] / G. . Eke [et al.] // J. Mater. Sci.-Mater. Med. - 2014. - Vol. 25, Is. 6. - P1471-1481, DOI 10.1007/s10856-014-5169-5. - Cited References: 38. - The study was supported by the Government of the Russian Federation (Decree No. 220 of 09.04.2010) (Agreement No. 11.G34.31.0013) and (Grant No MD-3112.2012.4). We gratefully acknowledge the EC FP7 SKINTREAT project and the State Planning Organization (Turkey) for the grant to establish BIOMATEN. Mr. A. Buyuksungur is acknowledged for his contributions with CLSM. . - ISSN 0957-4530. - ISSN 1573-4838

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DRUG-DELIVERY
   PLGA NANOPARTICLES
   CELLULAR UPTAKE
   MICROPARTICLES
   POLYHYDROXYALKANOATES
   CYTOTOXICITY
   SIZE
   POLYESTERS
   MECHANISM
   CELLS
Аннотация: The purpose of this study was to develop micro and nano sized drug carriers from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and study the cell and skin penetration of these particles. PHBV micro/nanospheres were prepared by o/w emulsion method and were stained with a fluorescent dye, Nile Red. The particles were fractionated by centrifugation to produce different sized populations. Topography was studied by SEM and average particle size and its distribution were determined with particle sizer. Cell viability assay (MTT) was carried out using L929 fibroblastic cell line, and particle penetration into the cells were studied. Transdermal permeation of PHBV micro/nanospheres and tissue reaction were studied using a BALB/c mouse model. Skin response was evaluated histologically and amount of PHBV in skin was determined by gas chromatography-mass spectrometry. The average diameters of the PHBV micro/nanosphere batches were found to be 1.9 mu m, 426 and 166 nm. Polydispersity indices showed that the size distribution of micro sized particles was broader than the smaller ones. In vitro studies showed that the cells had a normal growth trend. MTT showed no signs of particle toxicity. The 426 and 166 nm sized PHBV spheres were seen to penetrate the cell membrane. The histological sections revealed no adverse effects. In view of this data nano and micro sized PHBV particles appeared to have potential to serve as topical and transdermal drug delivery carriers for use on aged or damaged skin or in cases of skin diseases such as psoriasis, and may even be used in gene transfer to cells.

WOS
Держатели документа:
[Eke, G.
Hasirci, N.
Hasirci, V.] Middle E Tech Univ, Dept Micro & Nanotechnol, TR-06800 Ankara, Turkey
[Eke, G.
Hasirci, N.
Hasirci, V.] METU Ctr Excellence Biomat & Tissue Engn, BIOMATEN, TR-06800 Ankara, Turkey
[Eke, G.] Ahi Evran Univ, Fac Arts & Sci, Dept Chem, TR-40100 Kirsehir, Turkey
[Kuzmina, A. M.
Shishatskaya, E. I.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Goreva, A. V.
Shishatskaya, E. I.] Inst Biophys SB RAS, Krasnoyarsk 66003, Russia
[Hasirci, N.
Hasirci, V.] Middle E Tech Univ, Dept Biotechnol, TR-06800 Ankara, Turkey
[Hasirci, N.
Hasirci, V.] Middle E Tech Univ, Dept Biomed Engn, TR-06800 Ankara, Turkey
[Hasirci, N.] Middle E Tech Univ, Dept Chem, TR-06800 Ankara, Turkey
[Hasirci, V.] Middle E Tech Univ, Dept Biol Sci, TR-06800 Ankara, Turkey
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Eke, G...; Kuzmina, A.M.; Goreva, A.V.; Shishatskaya, E.I.; Hasirci, N...; Hasirci, V...; Government of the Russian Federation [220, 11.G34.31.0013, MD-3112.2012.4]; EC FP7 SKINTREAT project; State Planning Organization (Turkey)

Найти похожие

16.

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria / M. A. Selivanova [et al.] // Cent. Eur. J. Biol. - 2014. - Vol. 9, Is. 10. - P951-959, DOI 10.2478/s11535-014-0331-0 . - ISSN 1644-3632
Кл.слова (ненормированные):
Am-241 -- Hormesis -- Luminous bacteria -- Peroxides -- Radiotoxicity -- Tritium
Аннотация: Effects of Americium-241 (241Am), alpha-emitting radionuclide of high specific radioactivity, and tritium (3H), beta-emitting radionuclide, on luminous bacteria Photobacterium phosphoreum were compared. Bioluminescence intensity served as a marker of bacterial physiological activity. Three successive stages in the bioluminescence response to 241Am and 3H were found under conditions of lowdose irradiation: (1) absence of effects, (2) activation, and (3) inhibition. They were interpreted in terms of bacterial response to stressfactor as stress recognition, adaptive response/syndrome, and suppression of physiological function (i.e. radiation toxicity). Times of bioluminescence activation (TBA) and inhibition (TBI) were suggested as parameters to characterize hormesis and toxic stages in a course of chronic low-dose irradiation of the microorganisms. Values of TBA and TBI of 241Am were shorter than those of 3H, revealing higher impact of alpha-irradiation (as compared to beta-irradiation) under comparable radiation doses. Increases of peroxide concentration and NADH oxidation rates in 241Am aquatic solutions were demonstrated; these were not found in tritiated water. The results reveal a biological role of reactive oxygen species generated in water solutions as secondary products of the radioactive decay. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha- and beta-emitting radionuclides in aquatic solutions. © 2014 Versita Warsaw and Springer-Verlag Wien.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, 660041 Krasnoyarsk, Russian Federation
Siberian State Technological University, Lesosibirsk, Krasnoyarsk region, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Selivanova, M.A.; Rozhko, T.V.; Devyatlovskaya, A.N.; Kudryasheva, N.S.

Найти похожие

17.

Experimental estimation of the possible use of submersed macrophytes for biotesting bottom sediments of the Yenisei River [Text] / T. A. Zotina [et al.] // Contemp. Probl. Ecol. - 2014. - Vol. 7, Is. 4. - P410-421, DOI 10.1134/S1995425514040131. - Cited References: 23. - We thank the staff of the Radioecology Laboratory and the Analytical Laboratory, Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, for gamma ray spectrometric and chemical analysis of the samples. This study is supported in part by the Russian Foundation for Basic Research, project no. 14-14-00076, and by "Biological Diversity" program no. 30.5 of the Presidium of the Russian Academy of Sciences. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
MYRIOPHYLLUM-SPICATUM
   TOXICITY
   SENSITIVITY
   GROWTH
   EXPOSURE
   ATRAZINE
   QUALITY
   WATERS
   TESTS
Кл.слова (ненормированные):
Elodea canadensis -- Myriophyllum spicatum -- bottom sediments -- toxicity -- technogenic radionuclides -- heavy metals -- biogens
Аннотация: The laboratory testing of bottom sediments (BSs) from the Yenisei River containing different concentrations of technogenic radionuclides, heavy metals, and biogenic elements (N and P) based on aquatic such plants as Elodea canadensis (Canadian waterweed) and Myriophyllum spicatum (Eurasian watermilfoil) has revealed a higher sensitivity of roots to the general quality of BSs than shoots: shoot length (9%) < root length (11%) < root number (15%) in M. spicatum; shoot length (22%) < root length (42%) < root number (44%) in E. canadensis. In contrast to M. spicatum, the growth parameters of roots and shoots in E. canadensis have differed in a significant statistical manner between most BS samples. A reverse correlation has been found between the increase in shoot length and the activity of technogenic radionuclides in BSs, which is mostly significant in E. canadensis (r (2) = 0.90-0.95, p = 0.05). Since the growth of shoots and roots in E. canadensis has turned out to be more sensitive to changes in the quality of BSs than that in M. spicatum, E. canadensis can be considered more prospective for biotesting BSs.

WOS
Держатели документа:
[Zotina, T. A.
Trofimova, E. A.
Bolsunovsky, A. Ya
Anishenko, O. V.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.A.; Trofimova, E.A.; Bolsunovsky, A.Y.; Anishenko, O.V.; Russian Foundation for Basic Research [14-14-00076]; "Biological Diversity" program of the Presidium of the Russian Academy of Sciences [30.5]

Найти похожие

18.

Cytogenetic abnormalities in aquatic plant Elodea canadensis in anthropogenic contamination zone of Yenisei River [Text] / M. Y. Medvedeva, A. Y. Bolsunovsky, T. A. Zotina // Contemp. Probl. Ecol. - 2014. - Vol. 7, Is. 4. - P422-432, DOI 10.1134/S1995425514040088. - Cited References: 19. - The authors thank their colleagues from the Radioecology laboratory and Analytical laboratory for gamma spectrometric and chemical analyses. The authors express their appreciation to O.V. Kvitko (Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk) for her valuable methodical consultations. This research is partially funded by both a RFFI-r_Siberia-a, grant no. 13-04-98004 from the Russian Foundation for Basic Research and project no. 30.5 of the Biological Diversity Program of the Presidium of the Russian Academy of Sciences. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
ALLIUM-TEST
GENOTOXICITY
TOXICITY
Кл.слова (ненормированные):
Elodea canadensis -- bottom sediments -- chromosome abnormalities -- anthropogenic radionuclides -- heavy metals -- genotoxicity
Аннотация: Chromosome abnormalities in ana-telophase cells of apical root meristem of aquatic plant Elodea. canadensis (elodea), sampled in 2011-2012 in the Yenisei River at a site with background level of contamination and at several sites on the stretch contaminated with artificial radionuclides, and with chemical pollutants from municipal and industrial discharges of the Krasnoyarsk city. Lowest rate (5.2%) of cells with chromosome abnormalities was registered at sampling site with background level of contamination upstream of the Krasnoyarsk, highest rate of cells with abnormalities (39.7%)-in roots of elodea sampled in bottom sediments with highest concentration of Cs-137. Sum of rates of cells with abnormalities and rates of cells with all types of abnormalities positively correlated with total concentration of artificial and natural radionuclides, with concentration of artificial radionuclides and Cs-137 in bottom sediments of the Yenisei River (r (2) = 0.91-0.96, p < 0.0005 for sum of rates of cells with abnormalities; r (2) = 0.58-0.92, p < 0.05 for all types of abnormalities).

WOS
Держатели документа:
[Medvedeva, M. Yu
Bolsunovsky, A. Ya
Zotina, T. A.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, M.Y.; Bolsunovsky, A.Y.; Zotina, T.A.; RFFI-r_Siberia-a from the Russian Foundation for Basic Research [13-04-98004]; Biological Diversity Program of the Presidium of the Russian Academy of Sciences [30.5]

Найти похожие

19.

Dobutamine prevents experimental postintoxication liver cirrhosis in mice / A. P. Vinokurov, A. V. Eremeev, N. A. Setkov // Bulletin of Experimental Biology and Medicine. - 2002. - Vol. 134, Is. 1. - P. 43-46, DOI 10.1023/A:1020604621142 . - ISSN 0007-4888
Кл.слова (ненормированные):
Dobutamine -- Hepatocytes -- Liver cirrhosis -- Proliferation -- adrenergic receptor stimulating agent -- alpha 1 adrenergic receptor stimulating agent -- beta 1 adrenergic receptor stimulating agent -- beta 2 adrenergic receptor stimulating agent -- carbon tetrachloride -- catecholamine derivative -- dobutamine -- epidermal growth factor -- animal cell -- animal experiment -- animal model -- animal tissue -- article -- cell activity -- cell culture -- cell death -- cell proliferation -- cell stimulation -- chronic hepatitis -- connective tissue -- controlled study -- disease association -- drug effect -- drug potency -- experimental test -- female -- liver cell -- liver cirrhosis -- liver fibrosis -- liver hyperplasia -- liver necrosis -- liver parenchyma -- liver regeneration -- liver toxicity -- morphology -- mouse -- nonhuman -- toxicity testing -- Adrenergic beta-Agonists -- Animals -- Carbon Tetrachloride -- Cell Division -- Dobutamine -- Dose-Response Relationship, Drug -- Female -- Hepatocytes -- Liver -- Liver Cirrhosis -- Mice -- Mice, Inbred BALB C -- Animalia
Аннотация: Various chronic inflammatory and necrotic processes in the liver parenchyma are accompanied by pathological morphofunctional changes, which are associated with hepatocyte death and hyperplasia of the connective tissue. Regeneration of the liver parenchyma should include not only prevention of fibrosis, but also stimulation of hepatocyte prolifearation. The adrenoceptor agonist dobutamine stimulated proliferative activity of cultured hepatocytes and prevented the development of postintoxication liver cirrhosis in mice produced by chronic poisoning with CCl4.

Scopus
Держатели документа:
Department of General Surgery, Krasnoyarsk State Medical Academy, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vinokurov, A.P.; Eremeev, A.V.; Setkov, N.A.

Найти похожие

20.

Luminous bacteria: Biotechnological aspects [Text] / G. A. Vydryakova [et al.] ; ed. A Tsuji [et al.] // Bioluminescence & Chemiluminescence: Progress and Perspectives : WORLD SCIENTIFIC PUBL CO PTE LTD, 2005. - 13th International Symposium on Bioluminescence and Chemiluminescence (AUG 02-06, 2004, Yokohama, JAPAN). - P. 95-98, DOI 10.1142/9789812702203_0022. - Cited References: 12 . - ISBN 981-256-118-8

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Chemistry, Applied + Chemistry, Physical + Optics
Рубрики:
TOXICITY
WATER

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vydryakova, G.A.; Chugaeva, Y.V.; Tyulkova, N.A.; Medvedeva, S.E.; Kuznetsov, A.M.; Rodicheva, E.K.; Tsuji, A \ed.\; Matsurnoto, M \ed.\; Maeda, M \ed.\; Kricka, LJ \ed.\; Kricka,, J \ed.\

Найти похожие