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Influence of high concentrations of mineral salts on production process and NaCl accumulation by Salicornia europaea plants as a constituent of the LSS phototroph link / N. A. Tikhomirova [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1589-1593, DOI 10.1016/j.asr.2005.01.055 . - ISSN 0273-1177
Кл.слова (ненормированные):
BLSS -- Intensity of PAR -- Mineral nutrition -- Productivity -- Salicornia europaea -- Biomass -- Concentration (process) -- Nitrogen -- Nutrition -- Photosynthesis -- Productivity -- Sodium chloride -- Bioregenerative life support systems (BLSS) -- Intensity of PAR -- Mineral nutrition -- Salicornia europaea -- Plants (botany) -- calcium -- magnesium -- nitrogen -- phosphorus -- potassium -- sodium chloride -- sulfur -- urea -- biomass -- conference paper -- culture medium -- dose response -- drug effect -- goosefoot -- growth, development and aging -- human -- light -- metabolism -- microclimate -- radiation exposure -- urine -- Biomass -- Calcium -- Chenopodiaceae -- Culture Media -- Dose-Response Relationship, Drug -- Ecological Systems, Closed -- Humans -- Life Support Systems -- Light -- Magnesium -- Nitrogen -- Phosphorus -- Potassium -- Sodium Chloride -- Sulfur -- Urea -- Urine
Аннотация: Use of halophytes (salt-tolerant vegetation), in a particular vegetable Salicornia europaea plants which are capable of utilizing NaCl in rather high concentrations, is one of possible means of NaCl incorporation into mass exchange of bioregenerative life support systems. In preliminary experiments it was shown that S. europaea plants, basically, could grow on urine pretreated with physicochemical processing and urease-enzyme decomposing of urea with the subsequent ammonia distillation. But at the same time inhibition of the growth process of the plants was observed. The purpose of the given work was to find out the influence of excessive quantities of some mineral elements contained in products of physicochemical processing of urine on the production process and NaCl accumulation by S. europaea plants. As the content of mineral salts in the human liquid wastes (urine) changed within certain limits, two variants of experimental solutions were examined. In the first variant, the concentration of mineral salts was equivalent to the minimum salt content in the urine and was: K - 1.5 g/l, P - 0.5 g/l, S - 0.5 g/l, Mg - 0.07 g/l, Ca - 0.2 g/l. In the second experimental variant, the content of mineral salts corresponded to the maximum salt content in urine and was the following: K - 3.0 g/l, P - 0.7 g/l, S - 1.2 g/l, Mg - 0.2 g/l, Ca - 0.97 g/l. As the control, the Tokarev nutrient solution containing nitrogen in the form of a urea, and the Knop nutrient solution with nitrogen in the nitrate form were used. N quantity in all four variants made up 177 mg/l. Air temperature was 24 В°C, illumination was continuous. Light intensity was 690 ?mol/m2s of photosynthetically active radiation. NaCl concentration in solutions was 1%. Our researches showed that the dry aboveground biomass of an average plant of the first variant practically did not differ from the control and totaled 11 g. In the second variant, S. europaea productivity decreased and the dry aboveground biomass of an average plant totaled 8 g. The increase of K quantity in the experimental solutions resulted in an elevated content of the element in the plants. The increase of K uptake in the second experimental variant was accompanied by a 30-50% decrease of Na content in comparison with the other variants. Comparative Na content in the other variants was practically identical. N, Mg and P content in the control and experimental variants was also practically identical. The increase of S quantity in the second experimental variant also increased S uptake by the plants. But Ca quantity, accumulated in aboveground plants biomass in the experimental variants was lower than in the control. NaCl uptake by plants, depending on the concentration of mineral salts in the experimental solutions, ranged from 8 g (maximum salt content) up to 15 g (minimum salt content) on a plant growth area that totaled 0.032 m2. Thus, high concentrations of mineral salts simulating the content of mineral salts contained in urine did not result in a significant decrease of S. europaea productivity. The present work also considers the influence of higher light intensity concentrations on productivity and NaCl accumulation by S. europaea plants grown on experimental solutions with high salt content. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirova, N.A.; Ushakova, S.A.; Kovaleva, N.P.; Gribovskaya, I.V.; Tikhomirov, A.A.

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The possible way of introducing mineral elements of liquid human wastes into the material cycle in biological life support systems / A. A. Tikhomirov [et al.] // International Astronautical Federation - 55th International Astronautical Congress 2004. - 2004. - Vol. 3: International Astronautical Federation - 55th International Astronautical Congress 2004 (4 October 2004 through 8 October 2004, Vancouver) Conference code: 69653. - P1442-1448
Кл.слова (ненормированные):
Biomass -- Body fluids -- Hydrogen peroxide -- Life support systems (spacecraft) -- Solid wastes -- Biological life support systems -- Intrasystem material cycle -- Liquid human wastes -- Plant biomass -- Waste management
Аннотация: Along with the atmosphere, water and food regeneration processes in biological life support systems it is important to provide units and links responsible for utilization of unused plant biomass, human wastes and returning, if possible, the most of wastes into the intrasystem material cycle. The experience on construction of biological life support systems (BLSS) gained by the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) allows us to suggest constructing an integrated biological-physical-chemical life support system with the biological unit predominating. It is possibly to partially mineralize urine and solid wastes by "wet incineration" by hydrogen peroxide in electric field. We suggest decomposing urea by a urease-enzymatic method using soybean or canavalia flour containing sufficient amount of urease. Consumption of 1.5 g of flour for decomposition of urea in daily urine and the possibility of producing flour from soybeans and canavalia grown inside the system make this method of urea decomposition rather prospective. Further ammonia distillation using the nitrification unit and evaporation of solution would make possible to return nitrogen and water back into the intrasystem cycle. Probably, in long-duration space expeditions the utilization of urine would be confined only by extraction of nitrogen and water from urine with further removal of dry residue to the stock, as the problem of returning sodium chloride into the intrasystem cycling has not been solved yet. As all biogenic elements contained in urine (except nitrogen) get lost at that, the solution of the problem with introducing NaCl and mineral elements into the cycle with the help of halophyte plants Salicornia europaea are of sufficient interest. This work presents the experimental results of growing Salicornia europaea on model solutions containing biogenic elements in the amounts equivalent to their content in urine and on urine, which undergone physically-chemically treatment by peroxide and ammonia distillation after urease-enzymatic decomposition. Taking into consideration that the mineral elements content in urine can vary, 2 variants of model solutions were used. In the first variant the content of P was 8-fold, S - 7-fold, K - 8-fold higher than in Knop's solution; the content of Ca and Mg almost complied with that in Knop's solution. In the variant P was 12-fold, S - 17-fold, K - 17-fold, Ca - 6-fold and Mg was 8-fold higher than in Knop's solution. The content of N and NaCl in both variants was the same and constituted 0.18 g/l and 10 g/l respectively. The results of carried experiments showed that growing plants on urine treated in the above-mentioned way is possible; though the productivity of plants would be less than on model solutions. The reasons of plant productivity drop and the possible ways of their removal have been discussed.

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Держатели документа:
Institute of Biophysics, SB, RAS, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Gitelson, J.I.; Ushakova, S.A.; Kovaleva, N.P.; Tikhomirova, N.A.; Gribovskaya, I.V.

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Effect of photosynthetically active radiation, salinization, and type of nitrogen nutrition on growth of Salicornia europaea plants / S. A. Ushakova [et al.] // Russian Journal of Plant Physiology. - 2006. - Vol. 53, Is. 6. - P785-792, DOI 10.1134/S1021443706060094 . - ISSN 1021-4437
Кл.слова (ненормированные):
NaCl salinization -- Photosynthetically active radiation -- Productivity -- Salicornia europaea -- Types of nitrogen nutrition -- Salicornia europaea
Аннотация: Effects of various combinations of nutrient solution salinity (0.3, 171, and 342 mM NaCl), photosynthetically active radiation (PAR) of 600 or 1150 ?mol/(m2 s), and type of nitrogen nutrition (amide-N or nitrate-N) on the productivity and the content of accumulated mineral nutrients and free amino acids were studied in Salicornia europaea plants. At PAR of 600 ?mol/(m2 s), plant productivity increased with elevation of salinity level; at 1150 ?mol/(m2 s), the maximum productivity was observed in the plants grown at 171 mM of NaCl. The content of free amino acids in shoots, regardless of PAR, decreased with growing salinity level, whereas Na content, on the opposite, increased. Glutamic acid, rather than proline, was shown to be the main organic osmolyte in this plant species. Comparison of the productivity of plants grown on solutions with amide (urea) or nitrate nitrogen showed that higher biomass accumulation was achieved in the former case. В© 2006 Nauka/Interperiodica.

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Kovaleva, N.P.; Tikhomirova, N.A.; Gribovskaya, I.V.; Kolmakova, A.A.

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Direct utilization of human liquid wastes by plants in a closed ecosystem / G. M. Lisovsky [et al.] // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1801-1804 . - ISSN 0273-1177
Кл.слова (ненормированные):
fertilizer -- nitrogen -- sodium chloride -- urea -- article -- biomass -- culture medium -- feasibility study -- human -- metabolism -- methodology -- microclimate -- sewage -- urine -- waste management -- wheat -- Biomass -- Culture Media -- Ecological Systems, Closed -- Feasibility Studies -- Fertilizers -- Humans -- Nitrogen -- Sodium Chloride -- Triticum -- Urea -- Urine -- Waste Disposal, Fluid -- Waste Management
Аннотация: Model experiments in phytotrons have shown that urea is able to cover 70% of the demand in nitrogen of the conveyer cultivated wheat. At the same time wheat plants can directly utilize human liquid wastes. In this article by human liquid wastes the authors mean human urine only. In a long-term experiment on "man-higher plants" system with two crewmen, plants covered 63 m2, with wheat planted to - 39.6 m2. For 103 days, complete human urine (total amount - 210.7 1) wassupplied into the nutrient solution for wheat. In a month and a half NaCl supply into the nutrient solution stabilized at 0.9-1.65 g/l. This salination had no marked effect on wheat production. The experiment revealed the realistic feasibility to directly involve liquid wastes into the biological turnover of the life support system. The closure of the system, in terms of water, increased by 15.7% and the supply of nutrients for wheat plants into the system was decreased. Closedness of biological turnover of matter in a man-made "man - higher plants" ecological system might involve, among other processes, direct utilization of human liquid wastes by plants. The amount of urine comprises 15-20% of the total amount of water cycling within the system including water as part of food, household, hygiene and potable water necessary for man. What is more, it they contains most nitrogen-bearing compounds emitted by man, almost all of the NaCl and some other substances involved in the biological turnover. Human liquid wastes can be utilized either by preliminary physical-chemical treatment (evaporating or freezing out the water, finally oxidizing the organic matter, isolating the mineral components required for plants, etc.) and further involvement of the obtained products or by direct application into the nutrient solution for plants. The challenge of direct utilization is that plants have no need of Na+ and Cl-, and also the organic forms of nitrogen emitted by man cannot fully meet the demand of plants forthis element. Besides, hygienic and/or psychological reasons make it desirable to avoid direct use of liquid wastes in the nutrient solutions that would have direct contact with edible part of plants (tubers, roots, bulbs). Feasibility of direct utilization of liquid wastes by plants in a closed "man - higher plants" ecosystem has been experimentally studied on wheat - grain culture as a model plant with the edible part in the form of seeds spatially dissociated with the nutrient medium. The wheat covered 60-65% of the area under higher plants. The studies have been carried out in "Bios-3"experimental facility described in detail elsewhere (Lisovsky, 1979; Gitelson et al., 1989). В© 1997 COSPAR. Published by Elsevier Science Ltd.

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Держатели документа:
Inst. Biophys.-Russ. Acad. of Sci., Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lisovsky, G.M.; Gitelson, J.I.; Shilenko, M.P.; Gribovskaya, I.V.; Trubachev, I.N.

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Effects of mineral nutrition conditions on heat tolerance of chufa (Cyperus esculentus L.) plant communities to super optimal air temperatures in the BTLSS / E. S. Shklavtsova [et al.] // Adv. Space Res. - 2014. - Vol. 54, Is. 6. - P1135-1145, DOI 10.1016/j.asr.2014.05.031 . - ISSN 1879-1948
Кл.слова (ненормированные):
Bioregenerative life support system -- Chlorophyll fluorescence -- Cyperus esculentus L. -- Heat shock -- Lipid peroxidation -- Mineralized human wastes -- Atmospheric temperature -- Carbon dioxide -- Lipids -- Metabolism -- Minerals -- Nitrates -- Nitrogen -- Nutrition -- Plants (botany) -- Urea -- Wastes -- Bioregenerative life support systems -- Chlorophyll fluorescence -- Cyperus esculentus -- Heat-shock -- Human waste -- Lipid peroxidation -- Plant shutdowns
Аннотация: The use of mineralized human wastes as a basis for nutrient solutions will increase the degree of material closure of bio-technical human life support systems. As stress tolerance of plants is determined, among other factors, by the conditions under which they have been grown before exposure to a stressor, the purpose of the study is to investigate the level of tolerance of chufa (Cyperus esculentus L.) plant communities grown in solutions based on mineralized human wastes to a damaging air temperature, 45 °C. Experiments were performed with 30-day-old chufa plant communities grown hydroponically, on expanded clay aggregate, under artificial light, at 690 ?mol m-2 s-1 PAR and at a temperature of 25 °C. Plants were grown in Knop's solution and solutions based on human wastes mineralized according to Yu.A. Kudenko's method, which contained nitrogen either as ammonium and urea or as nitrates. The heat shock treatment lasted 20 h at 690 and 1150 ?mol m -2 s-1 PAR. Chufa heat tolerance was evaluated based on parameters of CO2 gas exchange, the state of its photosynthetic apparatus (PSA), and intensity of peroxidation of leaf lipids. Chufa plants grown in the solutions based on mineralized human wastes that contained ammonium and urea had lower heat tolerance than plants grown in standard mineral solutions. Heat tolerance of the plants grown in the solutions based on mineralized human wastes that mainly contained nitrate nitrogen was insignificantly different from the heat tolerance of the plants grown in standard mineral solutions. A PAR intensity increase from 690 ?mol m -2 s-1 to 1150 ?mol m-2 s-1 enhanced heat tolerance of chufa plant communities, irrespective of the conditions of mineral nutrition under which they had been grown. © 2014 COSPAR. Published by Elsevier Ltd. All rights reserved.

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Держатели документа:
SB RAS Institute of Biophysics, 660036 Akademgorodok, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shklavtsova, E.S.; Ushakova, S.A.; Shikhov, V.N.; Anishchenko, O.V.

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Effects of mineral nutrition conditions on heat tolerance of chufa (Cyperus esculentus L.) plant communities to super optimal air temperatures in the BTLSS [Text] / E. S. Shklavtsova [et al.] // Adv. Space Res. - 2014. - Vol. 54, Is. 6. - P1135-1145, DOI 10.1016/j.asr.2014.05.031. - Cited References: 26. - The study was performed within the framework of the program of fundamental research for the Russian academies of sciences for 2013-2020, subject No. 56.1.4. . - ISSN 0273-1177. - ISSN 1879-1948

РУБ Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
LIFE-SUPPORT-SYSTEMS
   CHLOROPHYLL FLUORESCENCE
   STRESS
   WASTE
   WHEAT
   LSS
Кл.слова (ненормированные):
Bioregenerative life support system -- Cyperus esculentus L. -- Heat shock -- Mineralized human wastes -- Chlorophyll fluorescence -- Lipid peroxidation
Аннотация: The use of mineralized human wastes as a basis for nutrient solutions will increase the degree of material closure of bio-technical human life support systems. As stress tolerance of plants is determined, among other factors, by the conditions under which they have been grown before exposure to a stressor, the purpose of the study is to investigate the level of tolerance of chufa (Cyperus esculentus L.) plant communities grown in solutions based on mineralized human wastes to a damaging air temperature, 45 degrees C. Experiments were performed with 30-day-old chufa plant communities grown hydroponically, on expanded clay aggregate, under artificial light, at 690 mu mol m(-2) s(-1) PAR and at a temperature of 25 degrees C. Plants were grown in Knop's solution and solutions based on human wastes mineralized according to Yu.A. Kudenko's method, which contained nitrogen either as ammonium and urea or as nitrates. The heat shock treatment lasted 20 h at 690 and 1150 mu mol m(-2) s(-1) PAR. Chufa heat tolerance was evaluated based on parameters of CO2 gas exchange, the state of its photosynthetic apparatus (PSA), and intensity of peroxidation of leaf lipids. Chufa plants grown in the solutions based on mineralized human wastes that contained ammonium and urea had lower heat tolerance than plants grown in standard mineral solutions. Heat tolerance of the plants grown in the solutions based on mineralized human wastes that mainly contained nitrate nitrogen was insignificantly different from the heat tolerance of the plants grown in standard mineral solutions. A PAR intensity increase from 690 mu mol m(-2) s(-1) to 1150 mu mol m(-2) s(-1) enhanced heat tolerance of chufa plant communities, irrespective of the conditions of mineral nutrition under which they had been grown. (C) 2014 COSPAR. Published by Elsevier Ltd. All rights reserved.

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Держатели документа:
[Shklavtsova, E. S.
Ushakova, S. A.
Shikhov, V. N.
Anishchenko, O. V.] SB RAS Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shklavtsova, E.S.; Ushakova, S.A.; Shikhov, V.N.; Anishchenko, O.V.; program of fundamental research for the Russian academies of sciences [56.1.4]

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Designing a reusable system based on nanodiamonds for biochemical determination of urea / N. O. Ronzhin [et al.] // Doklad. Biochem. Biophys. - 2015. - Vol. 465, Is. 1. - P428-431, DOI 10.1134/S1607672915060216 . - ISSN 1607-6729
Аннотация: A reusable system including urease covalently bound to the surface of modified nanodiamonds (MNDs) has been developed for the multiple determination of urea. The immobilized enzyme exhibits functional activity and catalyzes the hydrolysis of urea to yield ammonia. The presence of ammonia is confirmed by the formation of a colored product after the addition of chemical reagents. It was shown that the MNDs-urease complex can function in a wide range of temperatures and pH as well as in deionized water. The complex provides a linear yield of the product at low analyte concentrations and allows the multiple determination of urea in vitro. © 2015, Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Ronzhin, N. O.; Baron, A. V.; Bondar, V. S.; Gitelson, I. I.

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Biodegradable poly-3-hydroxybutyrate as a fertiliser carrier / T. G. Volova, S. V. Prudnikova, A. N. Boyandin // J. Sci. Food Agric. - 2016. - P4183-4193, DOI 10.1002/jsfa.7621 . - ISSN 0022-5142
Кл.слова (ненормированные):
biodegradable polymers -- controlled delivery systems -- fertilisers -- polyhydroxyakanoates
Аннотация: BACKGROUND: Increasing use of mineral fertilisers can lead to accumulation of fertilisers in soil, water and foodstuffs. One of the approaches to preventing these problems is to develop controlled release forms of fertilisers. RESULTS: Experimental formulations of the nitrogen fertiliser urea loaded in a degradable matrix of the natural polymer poly-3-hydroxybutyrate (P3HB) in the form of films, pellets and coated granules were constructed and investigated. Nitrogen release into soil occurred as the polymer was degraded, and it was dependent on the geometry of the carrier and the amount of nitrogen loaded in it, showing that nitrogen release can last for 30 days or longer and that release rates can be controlled by varying the fabrication technique employed. P3HB/urea formulations have a favourable effect on the soil microbial community. The use of embedded urea has a beneficial influence on the growth of creeping bentgrass (Agrostis stolonifera) and lettuce (Latuca sativa) and reduces removal of nitrogen with drain water. CONCLUSION: The slow-release nitrogen formulations developed in this study can be buried in soil together with seeds preventing nitrogen deficiency. The use of such slow-release formulations can decrease the amounts of chemicals in the environment and prevent their adverse effects on the biosphere. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry

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Держатели документа:
Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Prudnikova, S. V.; Boyandin, A. N.

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Experimental approach to study the effect of mutations on the protein folding pathway / E. V. Nemtseva [et al.] // PLoS One. - 2019. - Vol. 14, Is. 1. - Ст. e0210361, DOI 10.1371/journal.pone.0210361. - Cited References:38. - The study of time-resolved protein fluorescence was supported by the Ministry of Science and Education of the Russian Federation (Projects 6.7734.2017). The investigation of protein fluorescence and genetic engineering studies of bovine carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The study of time-resolved protein fluorescence was supported by the Ministry of Science and Education of the Russian Federation (Project 6.7734.2017). The investigation of protein fluorescence and genetic engineering studies of bovine carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
FLUORESCENCE LIFETIMES ORIGIN
   TRANSITION-STATE
   EXCHANGE
   TRYPTOPHAN
Аннотация: Is it possible to compare the physicochemical properties of a wild-type protein and its mutant form under the same conditions? Provided the mutation has destabilized the protein, it may be more correct to compare the mutant protein under native conditions to the wild-type protein destabilized with a small amount of the denaturant. In general, is it appropriate to compare the properties of proteins destabilized by different treatments: mutations, pH, temperature, and denaturants like urea? These issues have compelled us to search for methods and ways of presentation of experimental results that would allow a comparison of mutant forms of proteins under different conditions and lead to conclusions on the effect of mutations on the protein folding/unfolding pathway. We have studied equilibrium unfolding of wild-type bovine carbonic anhydrase II (BCA II) and its six mutant forms using different urea concentrations. BCA II has been already studied in detail and is a good model object for validating new techniques. In this case, time-resolved fluorescence spectroscopy was chosen as the basic research method. The main features of this experimental method allowed us to compare different stages of unfolding of studied proteins and prove experimentally that a single substitution of the amino acid in three mutant forms of BCA II affected the native state of the protein but did not change its unfolding pathway. On the contrary, the inserted disulfide bridge in three other mutant forms of BCA II affected the protein unfolding pathway. An important result of this research is that we have validated the new approach allowing investigation of the effect of mutations on the folding of globular proteins, because in this way it is possible to compare proteins in the same structural states rather than under identical conditions.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Prot Res, Pushchino, Moscow Region, Russia.

Доп.точки доступа:
Nemtseva, Elena V.; Gerasimova, Marina A.; Melnik, Tatiana N.; Melnik, Bogdan S.; Gerasimova, Marina; Nemtseva, Elena; Ministry of Science and Education of the Russian Federation [6.7734.2017]; Russian Science Foundation [N14-24-00157]

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10.

Processing of household waste in the BTLSS using the wet combustion method / S. V. Trifonov, Y. A. Morozov, T. A. Kozlova // Life Sci. Space Res. - 2019. - Vol. 21. - P22-24, DOI 10.1016/j.lssr.2019.02.003 . - ISSN 2214-5524
Кл.слова (ненормированные):
Cellulose -- Hydrogen peroxide -- Organic waste -- Physicochemical processing of organic waste -- Urea -- cellulose -- hydrogen peroxide -- urea -- alternating current -- Article -- chemical procedures -- desalination -- dissolution -- domestic waste -- electric field -- household -- microclimate -- oxidation -- physical chemistry -- priority journal -- urine -- waste management -- wet combustion method
Аннотация: The present study discusses physicochemical methods of organic waste processing in closed biotechnical life support systems (BTLSS). Sanitary and household cotton wastes were processed by the method of wet combustion in hydrogen peroxide using an alternating current electric field – a promising physicochemical method for organic waste processing in the BTLSS. The highest efficiency of the process (in terms of power consumption, duration of the process, and oxidation rate) was achieved in experiments with oxidation of a combination of cotton fabrics and urea-containing wastes such as human urine and feces. The reason for this must be that urea is a reactive aqueous solvent of cellulose. © 2019

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Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Trifonov, S. V.; Morozov, Y. A.; Kozlova, T. A.

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11.

Structural transitions of photobacterium leiognathi luciferase determined by various optical techniques under urea-induced equilibrium denaturation / D. V. Gulnov [и др.] // Tsitologiya. - 2018. - Vol. 60, Is. 10. - С. 847-850, DOI 10.7868/S0041377118100181 . - ISSN 0041-3771
Кл.слова (ненормированные):
Bacterial luciferase -- Circular dichroism -- Denaturation -- Protein fluorescence lifetime -- Protein intermediate states
Аннотация: The study was aimed to identification of conformational transitions of Photobacterium leiognathi luciferase during equilibrium denaturation with urea using several optical techniques, including circular dichroism, stationary and time-resolved fluorescence. Gravity center and intensity ratio I 325 /I 390 for the fluorescence spectra, molar ellipticity at 222 nm and fluorescence lifetimes of the protein were analyzed. Investigated parameters revealed two possible transitions for P. leiognathi luciferase with the midpoints at 0.5—1.1 and 3.5—4.2 M of urea. Changes in the values of two lifetime components, characterizing the luciferase fluorescence reflect both transitions, while steady-state fluorescence parameters (gravity center of spectrum and I 325 /I 390 ratio) reveal only the second one. Far-UV circular dichroism spectra displayed transitions at 4.2 M of urea for P. leiognathi luciferase. Conformational transitions characteristics of P. leiognathi luciferase and previously studied Vibrio harveyi luciferase (Inlow et al., 2002) were compared. Since, according to the published data for V. harveyi, midpoint of the second conformational transition is at about 2.5 M of urea, the results indicate more stable secondary structure for the P. leiognathi luciferase under study. The possible reasons for observed differences in fluorescent characteristics of two types of luciferases during denaturation can be connected to the microenvironment variation of the tryptophan residues in their tertiary structure, namely in position 131 and 277 in a-subunit. © 2018 Sankt Peterburg. All rights reserved.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Gulnov, D. V.; Nemtseva, E. V.; Gerasimova, M. A.; Kratasyuk, V. A.

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12.

Bacterial luciferases from vibrio harveyi and photobacterium leiognathi demonstrate different conformational stability as detected by time-resolved fluorescence spectroscopy / E. V. Nemtseva, D. V. Gulnov, M. A. Gerasimova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 19. - Ст. 10449, DOI 10.3390/ijms221910449 . - ISSN 1661-6596
Кл.слова (ненормированные):
Bacterial luciferase -- Conforma-tional stability -- FRET -- Molecular dynamics -- Time-resolved spectroscopy -- Tryptophan fluorescence -- Unfolding pathway -- Urea-induced denaturation
Аннотация: Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite the fact that both of them are actively used as a reporter system. The aim of this study was to compare the conformational transitions of these luciferases from two different protein subfamilies during equilibrium unfolding with urea. Intrinsic steady-state and time-resolved fluorescence spectra and circular dichroism spectra were used to determine the stages of the protein unfolding. Molecular dynamics methods were applied to find the differences in the surroundings of tryptophans in both luciferases. We found that the unfolding pathway is the same for the studied luciferases. However, the results obtained indicate more stable tertiary and secondary structures of P. leiognathi luciferase as compared to enzyme from V. harveyi during the last stage of denaturation, including the unfolding of individual subunits. The distinctions in fluorescence of the two proteins are associated with differences in the structure of the C-terminal domain of ?-subunits, which causes different quenching of tryptophan emissions. The time-resolved fluorescence technique proved to be a more effective method for studying protein unfolding than steady-state methods. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Photobiology Laboratory, Institute of Biophysics SB RAS, Krasnoyarsk, 660036, Russian Federation
Institute of Protein Research, Russian Academy of Sciences, Pushchino, 142290, Russian Federation

Доп.точки доступа:
Nemtseva, E. V.; Gulnov, D. V.; Gerasimova, M. A.; Sukovatyi, L. A.; Burakova, L. P.; Karuzina, N. E.; Melnik, B. S.; Kratasyuk, V. A.

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13.

Bacterial Luciferases from Vibrio harveyi and Photobacterium leiognathi Demonstrate Different Conformational Stability as Detected by Time-Resolved Fluorescence Spectroscopy / E. V. Nemtseva, D. V. Gulnov, M. A. Gerasimova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 19. - Ст. 10449, DOI 10.3390/ijms221910449. - Cited References:45. - The research was partially funded by the Ministry of Science and Higher Education of the Russian Federation (projects No. FSRZ-2020-0006); by the RFBR and Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science (projects No. 20-44-243002 and 20-44-240006); and by the RFBR (project No. 20-34-90118). . - ISSN 1422-0067

РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
TRYPTOPHAN FLUORESCENCE
   CRYSTAL-STRUCTURE
   SUBUNIT
   BIOLUMINESCENCE
Кл.слова (ненормированные):
bacterial luciferase -- urea-induced denaturation -- time-resolved -- spectroscopy -- conformational stability -- FRET -- tryptophan fluorescence -- molecular dynamics -- unfolding pathway
Аннотация: Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite the fact that both of them are actively used as a reporter system. The aim of this study was to compare the conformational transitions of these luciferases from two different protein subfamilies during equilibrium unfolding with urea. Intrinsic steady-state and time-resolved fluorescence spectra and circular dichroism spectra were used to determine the stages of the protein unfolding. Molecular dynamics methods were applied to find the differences in the surroundings of tryptophans in both luciferases. We found that the unfolding pathway is the same for the studied luciferases. However, the results obtained indicate more stable tertiary and secondary structures of P. leiognathi luciferase as compared to enzyme from V. harveyi during the last stage of denaturation, including the unfolding of individual subunits. The distinctions in fluorescence of the two proteins are associated with differences in the structure of the C-terminal domain of alpha-subunits, which causes different quenching of tryptophan emissions. The time-resolved fluorescence technique proved to be a more effective method for studying protein unfolding than steady-state methods.

WOS
Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Photobiol Lab, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Prot Res, Pushchino 142290, Russia.

Доп.точки доступа:
Nemtseva, Elena, V; Gulnov, Dmitry, V; Gerasimova, Marina A.; Sukovatyi, Lev A.; Burakova, Ludmila P.; Karuzina, Natalya E.; Melnik, Bogdan S.; Kratasyuk, Valentina A.; Burakova, Lyudmila; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; RFBRRussian Foundation for Basic Research (RFBR) [20-34-90118]; Krasnoyarsk Regional Fund of Science [20-44-243002, 20-44-240006]; RFBRRussian Foundation for Basic Research (RFBR)

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14.

The use of the data derived from the PlanetScope satellite and unmanned aerial vehicles to estimate crop yield as dependent on the amount of nitrogen fertilizer applied / N. O. Malchikov, T. I. Pisman, I. Y. Botvich [et al.] // IOP Conference Series: Earth and Environmental Science : IOP Publishing Ltd, 2021. - Vol. 839: 5th International Scientific Conference on Agribusiness, Environmental Engineering and Biotechnologies, AGRITECH-V 2021 (16 June 2021 through 19 June 2021, ) Conference code: 172484, Is. 2. - Ст. 022004, DOI 10.1088/1755-1315/839/2/022004
Аннотация: The purpose of the present study is to show the usefulness of the satellite data and the data derived from unmanned aerial vehicles (UAVs) for estimating the relationship between cereal grain crop yield and the amount of nitrogen fertilizer applied. The study was conducted on the land of the Kuraginskoye Research Farm. The study material was spring barley cv. Biom. Three test plots were studied; mineral fertilizer, urea, was used in different quantities for foliar application in June; applications were performed at equal intervals. Multispectral images were based on PlanetScope satellite data, with the 3 m spatial resolution, and the data derived from the DJI Phantom 4 Multispectral UAV, with the 10 cm resolution. The satellite and UAV data were used to calculate spectral vegetation index (NDVI) (Normalized Difference Vegetation Index). A high correlation was obtained between the NDVI values calculated using satellite data and UAV data. The satellite data provided the basis for assessing barley crop yield as dependent on the amount of foliar-applied urea during the growing season. Maps of the spatial distribution of barley NDVI were constructed using the Phantom UAV data; they showed that the third foliar application of the fertilizer was not economically justified. © Published under licence by IOP Publishing Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Kuragino RF, FRC KSC SB RAS, Kuragino, Krasnoyarsk Krai, Russian Federation

Доп.точки доступа:
Malchikov, N. O.; Pisman, T. I.; Botvich, I. Y.; Emelyanov, D. V.; Vagner, V. V.; Shevyrnogov, A. P.

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