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полныйинформационныйкраткий
Поисковый запрос: (<.>S=ANTIBODIES<.>)
Общее количество найденных документов : 1
1.


   
    Bioluminescent aptamer-based sandwich-type assay of anti-myelin basic protein autoantibodies associated with multiple sclerosis / V. V. Krasitskaya [et al.] // Anal. Chim. Acta. - 2019. - Vol. 1064. - P112-118, DOI 10.1016/j.aca.2019.03.015. - Cited References:29. - This work was supported by the Russian Foundation for Basic Research (RFBR), Russia, under the grant No 17-315-50027; Russian State funded budget projects No. AAAA-A17-117013050026-4 and AAAA-A17-117020210021-7. . - ISSN 0003-2670. - ISSN 1873-4324
РУБ Chemistry, Analytical
Рубрики:
ANTIBODIES
   BIOMARKERS

   RNA

Кл.слова (ненормированные):
Bioluminescent microassay -- RNA aptamers -- Autoantibodies to myelin basic -- protein -- Multiple sclerosis
Аннотация: Bioluminescent solid-phase sandwich-type microassay was developed to detect multiple sclerosis (MS)-associated autoantibodies in human sera. The assay is based on two different 2'-F-Py RNA aptamers against the target autoantibodies as biospecific elements, and Ca2+-regulated photoprotein obelin as a reporter. The paper describes elaboration of the assay and its application to 91 serum samples from patients with clinically definite MS and 86 ones from individuals healthy in terms of MS. Based on the receiver-operator curve (ROC) analysis, the chosen threshold value as clinical decision limit offers sensitivity of 63.7% and specificity of 94.2%. The area under the ROC curve (AUC) value of 0.87 shows a good difference between the groups under investigation. The likelihood ratio of 10.97 proves the diagnostic value of the assay and its potential as one of the laboratory MS-tests. (C) 2019 Elsevier B.V. All rights reserved.

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Держатели документа:
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys SB, Krasnoyarsk 660036, Russia.
RAS, Inst Chem Biol & Fundamental Med SB, Novosibirsk 630090, Russia.
State Med Univ, Krasnoyarsk 660022, Russia.

Доп.точки доступа:
Krasitskaya, Vasilisa V.; Chaukina, Valentina V.; Abroskina, Maria V.; Vorobyeva, Maria A.; Ilminskaya, Aleksandra A.; Kabilov, Marsel R.; Prokopenko, Semyon V.; Nevinsky, Georgy A.; Venyaminova, Alya G.; Frank, Ludmila A.; Russian Foundation for Basic Research (RFBR), Russia [17-315-50027]; Russian State [AAAA-A17-117013050026-4, AAAA-A17-117020210021-7]

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