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Microbial degradation of polyhydroxyalkanoates in tropical soils / A. N. Boyandin [et al.] // Int. Biodeterior. Biodegrad. - 2013. - Vol. 83. - P77-84, DOI 10.1016/j.ibiod.2013.04.014. - Cited References: 74. - This study was financially supported by Project "Biotechnologies of novel biomaterials" (Agreement No. 11.G34.31.0013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning". . - 8. - ISSN 0964-8305

РУБ Biotechnology & Applied Microbiology + Environmental Sciences
Рубрики:
POLY-BETA-HYDROXYBUTYRATE
   CHAIN-LENGTH POLYHYDROXYALKANOATES
   POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE
   COASTAL WATERS
   PHA FILMS
   BIODEGRADATION
   BACTERIA
   IDENTIFICATION
   POLYESTERS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
Кл.слова (ненормированные):
Polyhydroxyalkanoates (PHAs) -- Biopolymers -- Biopolymer properties -- Soil biodegradation -- PHA degrading microorganisms
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. (C) 2013 Elsevier Ltd. All rights reserved.

Держатели документа:
[Boyandin, Anatoly N.
Volova, Tatiana G.
Gitelson, Iosif I.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Prudnikova, Svetlana V.
Volova, Tatiana G.] Siberian Fed Univ, Krasnoyarsk, Russia
[Karpov, Valery A.] Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Moscow, Russia
[Ivonin, Vladimir N.
Ngoc Lanh Do
Thi Hoai Nguyen
Thi My Hiep Le
Filichev, Nikolay L.
Levin, Alexander L.] Joint Russian Vietnam Trop Res & Test Ctr, Hanoi, Vietnam
[Filipenko, Maxim L.] Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Novosibirsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Prudnikova, S.V.; Karpov, V.A.; Ivonin, V.N.; Do, N.L.; Nguyen, T.H.; Le, TMH; Filichev, N.L.; Levin, A.L.; Filipenko, M.L.; Volova, T.G.; Gitelson, I.I.

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Degradable Polyhydroxyalkanoates as Herbicide Carriers [Text] / S. V. Prudnikova [et al.] // J. Polym. Environ. - 2013. - Vol. 21, Is. 3. - P675-682, DOI 10.1007/s10924-012-0561-z. - Cited References: 31. - We thank Dr. Christopher Brigham for critical review of the manuscript prior to submission. The study was supported by the project initiated by the Government of the Russian Federation for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (Agreement No. 11.G34.31.0013). . - 8. - ISSN 1566-2543

РУБ Engineering, Environmental + Polymer Science
Рубрики:
CONTROLLED-RELEASE
   SOIL
   BIODEGRADATION
   POLYESTERS
   PESTICIDE
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
   MICROSPHERES
   BACTERIA
Кл.слова (ненормированные):
Herbicide -- Haloxyfop-P-methyl -- Zellek Super -- Sustained-release formulations -- Polyhydroxyalkanoates -- Plant growth suppression
Аннотация: The biodegradable polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) has been used to design experimental sustained-release formulations of the herbicide Zellek Super in the form of films and microgranules. The kinetics of polymer degradation and the dynamics of herbicide release show that the rate and extent of herbicide release from the polymer matrix into the soil depends on the geometry of the carrier and the proportion of the pesticide loaded into it (polymer/pesticide mass ratio). Experiments with the creeping bentgrass (Agrostis stolonifera L.) show that the formulations of the herbicide Zellek Super constructed as microgranules and films can be successfully used to suppress the growth of grasses. This study is the first to demonstrate that biodegradable polyhydroxyalkanoates can be used effectively to construct environmentally friendly sustained-release PHA-herbicide systems that can be placed into the soil together with seeds.

Держатели документа:
[Prudnikova, S. V.
Sinskey, A. J.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Boyandin, A. N.
Kalacheva, G. S.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Sinskey, A. J.] MIT, Cambridge, MA 02139 USA : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Prudnikova, S.V.; Boyandin, A.N.; Kalacheva, G.S.; Sinskey, A.J.; Government of the Russian Federation [11.G34.31.0013]

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Biodegradation of polyhydroxyalkanoates by soil microbial communities of different structures and detection of PHA degrading microorganisms [Text] / A. N. Boyandin [et al.] // Appl. Biochem. Microbiol. - 2012. - Vol. 48, Is. 1. - P28-36, DOI 10.1134/S0003683812010024. - Cited References: 39. - The work was supported by the project initiated by the Government of the Russian Federation for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (Agreement No. 11.G34.31.0013) and the Program of Integrated Research of the Presidium of the Siberian Branch of Russian Academy of Sciences (project no. 96). . - 9. - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
POLY-BETA-HYDROXYBUTYRATE
   CHAIN-LENGTH POLYHYDROXYALKANOATES
   DEGRADATION
   FILMS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
   BACTERIA
   ACID
Аннотация: Biodegradation of microbial linear polymers of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) by soil microbial communities of different structures has been studied during two field seasons in different weather conditions. This process was shown to be influenced by the polymer chemical composition, temperature, humidity, and the microbial soil component. The PHA degradation was accompanied by a decrease in the polymer molecular weight and an increase in the degree of crystallinity, indicating the preferential destruction of the amorphous phase compared to the crystalline one. The quantity of the true PHA destructors developing at the surface of the polymer samples was lower than the quantity of accompanying bacteria. The dominant PHA degrading microorganisms under the test conditions were identified as bacteria of the genera Variovorax, Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus, and Xanthomonas and as micromycetes from Penicillium, Paecilomyces, Acremonium, Verticillium, and Zygosporium.

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Полный текст на сайте издателя
Держатели документа:
[Boyandin, A. N.
Volova, T. G.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Prudnikova, S. V.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Filipenko, M. L.
Khrapov, E. A.] Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia
[Vasil'ev, A. D.] Russian Acad Sci, Siberian Branch, Kirenskii Inst Phys, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A. N.; Prudnikova, S.V.; Filipenko, M.L.; Khrapov, E.A.; Vasiliev, A. D.; Васильев, Александр Дмитриевич; Volova, Tatiana G.; Волова, Татьяна Григорьевна; Government of the Russian Federation [11.G34.31.0013]; Presidium of the Siberian Branch of Russian Academy of Sciences [96]

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EFFECT OF CULTIVATION PARAMETERS ON THE LEVEL OF HYDROGENASE ACTIVITY IN ALCALIGENES-EUTROPHUS Z-1 CELLS [Text] / O. A. GUSEINOV, V. F. PLOTNIKOV // Microbiology. - 1991. - Vol. 60, Is. 2. - P. 144-148. - Cited References: 13 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
SOLUBLE HYDROGENASE
BACTERIA
Аннотация: The effect of different cultivation parameters on the specific activities of soluble and membrane-bound hydrogenases was studied in Alcaligenes eutrophus Z-1 cells. These activities were shown to be dependent primarily on the availability of electrons from the substrate used. Changes in cultivation temperature and oxygen concentration versus the optimal values as well as addition of low amounts of carbon monoxide to the gas phase exerted a lesser effect on the specific hydrogenase activities. Autotrophic and heterotrophic methods were developed for growing A. eutrophus Z-1 cells with a high hydrogenase activity.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GUSEINOV, O.A.; PLOTNIKOV, V.F.

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Stability of recombinant plasmids in transgenic microorganisms under different environmental conditions [Text] / L. Y. Popova [et al.] // Microbiology. - 2001. - Vol. 70, Is. 6. - P. 685-691, DOI 10.1023/A:1013187815739. - Cited References: 15 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
BACTERIA
Кл.слова (ненормированные):
recombinant plasmids -- transgenic microorganisms -- antibiotic resistance -- bioluminescence -- interferon
Аннотация: The copy number of R plasmids weakly depends on the selective pressure of the respective antibiotic but does depend on the physiology of the host species and the type of plasmids and cloned genes, whose expression leads to a further load on the biosynthetic apparatus of cells. The last factor is critical in the maintenance of recombinant plasmids in transgenic microorganisms.

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Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Y.; Maksimova, E.E.; Lobova, T.I.; Kargatova, T.V.; Boyandin, A.N.; Krylova, T.Y.; Pechurkin, N.S.

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Coexistence of genetically engineered Escherichia coli strains and natural microorganisms in experimental aquatic microcosms [Text] / T. V. Kargatova, E. E. Maksimova, L. Y. Popova // Microbiology. - 2001. - Vol. 70, Is. 2. - P. 211-216, DOI 10.1023/A:1010437731920. - Cited References: 17 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
SURVIVAL
BACTERIA
GROWTH
Кл.слова (ненормированные):
Escherichia coli -- aquatic microecosystems -- recombinant plasmid
Аннотация: In experimental aquatic microcosms (AMCs), the population of the Escherichia coli strain Z905 harboring the recombinant plasmid pPHL7 (Ap(r)Lux(+)) was found to gradually accumulate AMC-adapted cells, which retained the plasmid but differed from the original cells in some biochemical and physiological characteristics. Both the original and the AMC-adapted E. coil cells could coexist with the native AMC microflora for one year or longer. When introduced into AMCs together with native pseudomonads, the AMC-adapted E. coil Z905-33 (pPHL7) cells were more competitive than the nonadapted cells.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Popova, L.Y.

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Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems [Text] / E. E. Maksimova, L. Y. Popova ; ed. a, EE Maksim // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P. 1581-1586, DOI 10.1016/S0273-1177(01)00249-6. - Cited References: 17 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
MICROCOSMS
BACTERIA
Аннотация: Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maksimova, E.E.; Popova, L.Y.; Maksim, a, EE \ed.\

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Microbial processes of the carbon and sulfur cycles in Lake Shira (Khakasia) [Text] / N. V. Pimenov [et al.] // Microbiology. - 2003. - Vol. 72, Is. 2. - P. 221-229, DOI 10.1023/A:1023228316838. - Cited References: 17 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
BACTERIA
NOV
Кл.слова (ненормированные):
Lake Shira -- anoxygenic photosynthesis -- purple sulfur bacteria -- sulfate reduction -- production and oxidation of methane
Аннотация: Microbiological and biogeochemical studies of the meromictic saline Lake Shira (Khakasia) were conducted. In the upper part of the hydrogen-sulfide zone, at a depth of 13.5-14 m, there was a pale pink layer of water due to the development of purple bacteria (6 x 10(5) cells/ml), which were assigned by their morphological and spectral characteristics to Lamprocystis purpurea (formerly Amoebobacter purpureus). In August, the production of organic matter (OM) in Lake Shira was estimated to be 943 mg C/(m(2) day). The contribution of anoxygenic photosynthesis was insignificant (about 7% of the total OM production). The share of bacterial chemosynthesis was still less (no more than 2%). In the anaerobic zone, the community of sulfate-reducing bacteria played a decisive role in the terminal decomposition of OM. The maximal rates of sulfate reduction were observed in the near-bottom water (114 mug S/(1 day)) and in the surface layer of bottom sediments (901 mug S/(dm(3) day)). The daily expenditure of C-org for sulfate reduction was 73% of C-org formed daily in the processes of oxygenic and anoxygenic photosynthesis and bacterial chemosynthesis. The profile of methane distribution in the water column and bottom sediments was typical of meromictic reservoirs. The methane content in the water column increased beginning with the thermocline (7-8 m) and reached maximum values in the near-bottom water (17 mul/l). In bottom sediments, the greatest methane concentrations (57 mul/l) were observed in the surface layer (0-3 cm). The integral rate of methane formation in the water column and bottom sediments was almost an order of magnitude higher than the rate of its oxidation by aerobic and anaerobic methanotrophic microorganisms.

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Держатели документа:
Russian Acad Sci, Inst Microbiol, Moscow 117312, Russia
Tomsk VV Kuibyshev State Univ, Tomsk, Russia
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pimenov, N.V.; Rusanov, I.I.; Karnachuk, O.V.; Rogozin, D.Y.; Bryantseva, I.A.; Lunina, O.N.; Yusupov, S.K.; Parnachev, V.P.; Ivanov, M.V.

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Comparison of fatty acid composition in major lipid classes of the dominant benthic invertebrates of the Yenisei river [Text] / N. N. Sushchik [et al.] // Comp. Biochem. Physiol. B-Biochem. Mol. Biol. - 2003. - Vol. 134, Is. 1. - P. 111-122, DOI 10.1016/S1096-4959(02)00191-4. - Cited References: 30 . - ISSN 1096-4959

РУБ Biochemistry & Molecular Biology + Zoology
Рубрики:
LAKE-ERKEN
   BIOMARKERS
   BACTERIA
   MARKERS
   DIET
   FISH
   N-3
Кл.слова (ненормированные):
fatty acids -- triacylglycerols -- polar lipids -- gammarids -- aquatic larvae of insects
Аннотация: The composition and content of fatty acids (FAs) in total lipids, triacylglycerols (TAG) and polar lipids (PL) in dominant groups of benthic invertebrates: gammarids (Gammaridae, Amphipoda), chironomid larvae (Chironomidae, Diptera), caddisfly larvae (Trichoptera) and mayfly larvae (Ephemeroptera) were studied in the Yenisei river. For the first time data on the FA composition of species belonging to Trichoptera (Insecta) are presented. The groups of aquatic insect larvae and gammarids weakly differed in total content of essential polyunsaturated fatty acids (PUFAs). Hence, the strong invasion of gammarids which occurred in the last decades in the Yenisei river should not result in a decrease in potential yield of essential PUFA in the ecosystem and corresponding decrease in food resource quality for fish in respect to PUFA content. Significant differences in biomarker FAs in TAG were found which correlated to specific food sources. Different levels of long-chain PUFA in PL of the invertebrates are discussed in relation to the genetic ability of particular taxa to form these FAs. (C) 2002 Elsevier Science Inc. All rights reserved.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Gladyshev, M.I.; Moskvichova, A.V.; Makhutova, O.N.; Kalachova, G.S.

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10.

Antioxidant activity of humic substances via bioluminescent monitoring in vitro [Text] / A. S. Tarasova, D. I. Stom, N. S. Kudryasheva // Environ. Monit. Assess. - 2015. - Vol. 187, Is. 3. - Ст. 89, DOI 10.1007/s10661-015-4304-1. - Cited References:51. - This work was supported by the Russian Foundation for Basic Research, Grant No. 15-03-06786a, the Program "Molecular and Cellular Biology" of the Russian Academy of Sciences, project VI 57.1.1. . - ISSN 0167-6369. - ISSN 1573-2959

РУБ Environmental Sciences
Рубрики:
DETOXIFICATION PROCESSES
   TOXICITY
   BIOASSAYS
   BACTERIA
   ASSAY
Кл.слова (ненормированные):
Antioxidant activity -- Oxidative toxicity -- General toxicity -- Humic -- substances -- Bioassay -- Bioluminescence
Аннотация: This work considers antioxidant properties of natural detoxifying agents-humic substances (HS) in solutions of model inorganic and organic compounds of oxidative nature-complex salt K-3[Fe(CN)(6)] and 1,4-benzoquinone. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P) H:FMN-oxidoreductase and bacterial luciferase was used as a bioassay in vitro to monitor toxicity of the oxidizer solutions. Toxicities of general and oxidative types were evaluated using bioluminescent kinetic parameters-bioluminescence intensity and induction period, respectively. Antioxidant activity of HS was attributed to their ability to decrease both general and oxidative toxicities; the HS antioxidant efficiency was characterized with detoxification coefficients D-GT and D-OxT, respectively. Dependencies of D-GT and D-OxT on HS concentration and time of preliminary incubation of the oxidizers with HS were demonstrated. The optimal conditions for detoxification of the oxidizers were >20-min incubation time and 0.5x10(-4) to 2x10(-4) M of HS concentration. The present study promotes application of the enzymatic luminescent bioassay to monitor toxicity of pollutants of oxidative nature in environmental and waste waters in remediation procedures.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Irkutsk State Univ, Irkutsk 664003, Russia.
ИБФ СО РАН

Доп.точки доступа:
Tarasova, A.S.; Stom, D.I.; Kudryasheva, N.S.; Russian Foundation for Basic Research [15-03-06786a]; Russian Academy of Sciences [VI 57.1.1]

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