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Формат представления найденных документов:
полный	информационный	краткий

Поисковый запрос: (<.>S=EXERCISE<.>)

Общее количество найденных документов : 1

A noninvasive and qualitative bioluminescent assay for express diagnostics of athletes' responses to physical exertion / V. A. Kratasyuk, L. V. Stepanova, R. Ranjan [et al.] // Luminescence. - 2020, DOI 10.1002/bio.3954. - Cited References:33. - The Ministry of Science and Higher Education of the Russian Federation, Grant/Award Number: FSRZ-2020-0006; Krasnoyarsk Regional Foundation of Science, Grant/Award Number: KF-537 . - Article in press. - ISSN 1522-7235. - ISSN 1522-7243

РУБ Chemistry, Analytical
Рубрики:
SALIVARY BIOMARKERS
EXERCISE
Кл.слова (ненормированные):
athletes -- BLuc‐ -- Red coupled enzyme system -- catalase activity -- saliva -- training load
Аннотация: Upcoming professional sports authorities seek rapid noninvasive biosensing tools for regular monitoring of athletes' physiological states. The analysis of saliva through luminescence-based biosensors has been perceived as a suitable candidate for such purposes. The present study reports a qualitative bioluminescence assay based on a coupled enzyme system that consists of bacterial luciferase (BLuc) and nicotinamide adenine dinucleotide (NADH):flavin mononucleotide (FMN) oxidoreductase (Red), BLuc-Red, for the express diagnostics of athletes' stress levels before and after physical exertion. The volunteers who participated in the study were grouped as freestyle wrestlers and students who adapted to different levels of physical activities. Under physical exertion modelling conditions, the influence of participant saliva on BLuc-Red catalyzed light emission was investigated. Results showed a significant increase in residual luminescence (I-exp, mean maximum bioluminescence intensity of the experimental measurement (I-exp); I-c, luminescence intensity in control; I-exp/I-c, %) values for participants in the wrestler group while a decrease in the student group (P < 0.05). Such contrasting residual luminescence values in both groups were found to be dependent on the catalase activity of saliva. The proposed bioluminescence assay can be utilized as a potential nonspecific biosensing tool for determining the physical state of athletes under high loads.

WOS
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Svobodny Prospect 79, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Akademgorodok 50-50, Akademgorodok, Russia.
Krasnoyarsk State Med Univ, Minist Hlth Russian Federat, Av Partizan Zheleznyak 1, Krasnoyarsk, Russia.
Krasnoyarsk Matern & Childhood Protect Ctr, Kirenskogo St 2a, Krasnoyarsk, Russia.
Siberian Fed Univ, Sch Nonferrous Met & Mat Sci, Svobodny Prospect 79, Krasnoyarsk, Russia.
Sci Res Inst Med Problems North, Av Partizan Zheleznyak 3g, Krasnoyarsk, Russia.

Доп.точки доступа:
Kratasyuk, Valentina A.; Stepanova, Lyudmila, V; Ranjan, Rajeev; Sutormin, Oleg S.; Pande, Shubhra; Zhukova, Galina, V; Miller, Olga M.; Maznyak, Natalya, V; Kolenchukova, Oksana A.; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; Krasnoyarsk Regional Foundation of Science [KF-537]

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