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RESTING CELLS PREINCUBATED WITH ACTINOMYCIN-D DO NOT INHIBIT DNA-SYNTHESIS IN HETEROCARYONS WITH STIMULATED CELLS [Текст] / N. A. SETKOV, T. V. ANDREYEVA, V. N. KAZAKOV // DOKLADY AKADEMII NAUK SSSR. - 1991. - Vol. 318, Is. 4. - P. 986-987. - Cited References: 5 . - ISSN 0002-3264

РУБ Multidisciplinary Sciences
Рубрики:
NIH 3T3 CELLS
   NUCLEI
   HETERODIKARYONS
   FIBROBLASTS
   FUSION

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; ANDREYEVA, T.V.; KAZAKOV, V.N.

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THE ENTRY INTO S-PERIOD OF NUCLEI IN HETERODIKARYONS MODIFIED BY THE CYCLOHEXIMIDE [Текст] / N. A. SETKOV, V. N. KAZAKOV, T. V. ANDREEVA // TSITOLOGIYA. - 1991. - Vol. 33, Is. 12. - P. 73-78. - Cited References: 16 . - ISSN 0041-3771

РУБ Cell Biology
Рубрики:
NIH 3T3 CELLS
   DNA-SYNTHESIS
   RESTING CELLS
   C-MYC
   FUSION
   FIBROBLASTS
   EXPRESSION
   GENES
Аннотация: Serum-deprived (0.2%) resting NIH 3T3 mouse fibroblasts were fused with serum-stimulated (10%) proliferating cells to elucidate mechanisms of entering into S-period operating in the nuclei of the heterokaryons under the effect of cycloheximide - an inhibitor of protein synthesis. Using radioautography DNA synthesis was investigated in mono-, homo- and heterodikaryons. After short (0.5-3.0 h) depressing of protein synthesis, the nuclei of stimulated cells in heterokaryons were found to enter into S-period. Under these conditions no induction of DNA synthesis was found in the nuclei of resting cells in heterodikaryons. In other experiments, resting cells were under the effect of cycloheximide during 2-4 h before the fusion, that led to a great induction of DNA synthesis in the nuclei of these cells in heterodikaryons. The data obtained are consistent with the idea of fibroblast transition to the rest under the action of labile proteins-repressors.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; KAZAKOV, V.N.; ANDREEVA, T.V.

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PROTEIN-SYNTHESIS INHIBITORS, LIKE GROWTH-FACTORS, MAY RENDER RESTING 3T3 CELLS COMPETENT FOR DNA-SYNTHESIS - A AUTORADIOGRAPHIC AND CELL-FUSION STUDY [Text] / N. A. SETKOV [et al.] // Cell Prolif. - 1992. - Vol. 25, Is. 3. - P. 181-191, DOI 10.1111/j.1365-2184.1992.tb01393.x. - Cited References: 20 . - ISSN 0960-7722

РУБ Cell Biology
Рубрики:
C-MYC
   CYCLOHEXIMIDE
   FIBROBLASTS
   EXPRESSION
   INDUCTION
   GENES
   FOS
Аннотация: Serum-deprived (0.1-0.2%) resting NIH 3T3 mouse fibroblasts pre-incubated with cycloheximide (7.5-mu-g/ml), or puromycin (10-mu-g/ml), were fused with stimulated cells taken 10 h after changing the medium to one containing 10% serum, and DNA synthesis was investigated in the nuclei of monokaryons, homodikaryons and heterodikaryons using radioautography with the double-labelling technique. Pre-incubation of resting cells with inhibitors of protein synthesis for 1-4 h abolished their ability to suppress DNA synthesis in stimulated nuclei in heterokaryons. Three hours after the removal of cycloheximide from the medium, the resting cells acquired once again the inhibitory capacity for entry of stimulated nuclei into the S period. This inhibitory influence disappeared also in the case of post-fusion cycloheximide application as well as following an 8-12 h pre-treatment of resting cells with actinomycin D (1-mu-g/ml) prior to fusion. Pre-incubation of resting cells for 12 h with PDGF (1 u/ml-1) followed by an 8-48 h incubation in serum-free medium stimulated the onset of DNA synthesis. A brief exposure (45 min) of resting cells to cycloheximide (7.5-mu-g/ml), or puromycin (7.5-mu-g/ml), exerted a similar effect, inducing by itself the entry of cells into the S period. The results support the assumption that acquirement, by resting cells, of competence for DNA replication includes as a necessary step the down-regulation of intracellular growth inhibitors whose formation depends on protein synthesis.

WOS
Держатели документа:
ACAD SCI USSR,INST BIOPHYS,KRASNOYARSK,USSR
WA ENGELHARDT MOLEC BIOL INST,MOSCOW,USSR
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; KAZAKOV, V.N.; ROSENWALD, I.B.; MAKAROVA, G.F.; EPIFANOVA, O.I.

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APROTININ ACTION ON DNA-SYNTHESIS IN THE NUCLEI OF HETEROKARYONS FORMED BY FUSION RESTING AND STIMULATED NIH 3T3 CELLS [Текст] / N. A. SETKOV, V. N. KAZAKOV // Dokl. Akad. Nauk. - 1993. - Vol. 333, Is. 3. - P. 398-401. - Cited References: 12 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
FIBROBLASTS
GROWTH
AUTOPHAGY

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; KAZAKOV, V.N.

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APROTININ SUPPRESSED ACTION CYCLOHEXIMIDE ON THE DNA-SYNTHESIS IN NUCLEI OF HETEROKARYONS FORMED BY FUSION RESTING AND STIMULATED NIH 3T3 CELLS [Текст] / N. A. SETKOV // Dokl. Akad. Nauk. - 1994. - Vol. 339, Is. 2. - P. 269-271. - Cited References: 13 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
HETERODIKARYONS
   FIBROBLASTS
   INHIBITORS
   AUTOPHAGY

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.

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OKADAIC ACID IS AN INHIBITOR OF TYPE-1 AND TYPE 2A PROTEIN PHOSPHATASES STIMULATED DNA-SYNTHESIS IN RESTING CELLS NIH 3T3 [Текст] / N. A. SETKOV // Dokl. Akad. Nauk. - 1995. - Vol. 340, Is. 1. - P. 114-118. - Cited References: 15 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
PHOSPHORYLATION
   NUCLEI
   FUSION
   HETERODIKARYONS
   PROLIFERATION
   FIBROBLASTS
   CYCLIN

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.

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Protein synthesis inhibitors stimulated DNA synthesis in resting cells NIT 3T3 [Текст] / N. A. Setkov // Dokl. Akad. Nauk. - 1996. - Vol. 347, Is. 4. - P. 552-555. - Cited References: 15 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
GROWTH
   FIBROBLASTS
   NUCLEI
   IDENTIFICATION
   CYCLOHEXIMIDE
   FUSION
   PERIOD
   STATE

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.

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Negative control of cell proliferation depend on protein synthesis in resting cells [Текст] / N. A. Setkov // Dokl. Akad. Nauk. - 1996. - Vol. 346, Is. 5. - P. 708-711. - Cited References: 15 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
C-FOS
   SYNTHESIS INHIBITORS
   3T3 CELLS
   TRANSCRIPTIONAL ACTIVATION
   DNA-SYNTHESIS
   FIBROBLASTS
   NUCLEI
   FUSION
   MYC
   JUN

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.

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Liposomal cysteine proteinases inhibitors can commitment resting NIH 3T3 cells to proliferate [Текст] / N. A. Setkov // Dokl. Akad. Nauk. - 1997. - Vol. 354, Is. 3. - P. 405-408. - Cited References: 14 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
FIBROBLASTS
   GROWTH
   DEGRADATION
   TURNOVER

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.

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