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RESTING CELLS PREINCUBATED WITH ACTINOMYCIN-D DO NOT INHIBIT DNA-SYNTHESIS IN HETEROCARYONS WITH STIMULATED CELLS [Текст] / N. A. SETKOV, T. V. ANDREYEVA, V. N. KAZAKOV // DOKLADY AKADEMII NAUK SSSR. - 1991. - Vol. 318, Is. 4. - P. 986-987. - Cited References: 5 . - ISSN 0002-3264

РУБ Multidisciplinary Sciences
Рубрики:
NIH 3T3 CELLS
   NUCLEI
   HETERODIKARYONS
   FIBROBLASTS
   FUSION

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; ANDREYEVA, T.V.; KAZAKOV, V.N.

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THE ENTRY INTO S-PERIOD OF NUCLEI IN HETERODIKARYONS MODIFIED BY THE CYCLOHEXIMIDE [Текст] / N. A. SETKOV, V. N. KAZAKOV, T. V. ANDREEVA // TSITOLOGIYA. - 1991. - Vol. 33, Is. 12. - P. 73-78. - Cited References: 16 . - ISSN 0041-3771

РУБ Cell Biology
Рубрики:
NIH 3T3 CELLS
   DNA-SYNTHESIS
   RESTING CELLS
   C-MYC
   FUSION
   FIBROBLASTS
   EXPRESSION
   GENES
Аннотация: Serum-deprived (0.2%) resting NIH 3T3 mouse fibroblasts were fused with serum-stimulated (10%) proliferating cells to elucidate mechanisms of entering into S-period operating in the nuclei of the heterokaryons under the effect of cycloheximide - an inhibitor of protein synthesis. Using radioautography DNA synthesis was investigated in mono-, homo- and heterodikaryons. After short (0.5-3.0 h) depressing of protein synthesis, the nuclei of stimulated cells in heterokaryons were found to enter into S-period. Under these conditions no induction of DNA synthesis was found in the nuclei of resting cells in heterodikaryons. In other experiments, resting cells were under the effect of cycloheximide during 2-4 h before the fusion, that led to a great induction of DNA synthesis in the nuclei of these cells in heterodikaryons. The data obtained are consistent with the idea of fibroblast transition to the rest under the action of labile proteins-repressors.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; KAZAKOV, V.N.; ANDREEVA, T.V.

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OKADAIC ACID IS AN INHIBITOR OF TYPE-1 AND TYPE 2A PROTEIN PHOSPHATASES STIMULATED DNA-SYNTHESIS IN RESTING CELLS NIH 3T3 [Текст] / N. A. SETKOV // Dokl. Akad. Nauk. - 1995. - Vol. 340, Is. 1. - P. 114-118. - Cited References: 15 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
PHOSPHORYLATION
   NUCLEI
   FUSION
   HETERODIKARYONS
   PROLIFERATION
   FIBROBLASTS
   CYCLIN

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.

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Protein synthesis inhibitors stimulated DNA synthesis in resting cells NIT 3T3 [Текст] / N. A. Setkov // Dokl. Akad. Nauk. - 1996. - Vol. 347, Is. 4. - P. 552-555. - Cited References: 15 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
GROWTH
   FIBROBLASTS
   NUCLEI
   IDENTIFICATION
   CYCLOHEXIMIDE
   FUSION
   PERIOD
   STATE

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.

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Negative control of cell proliferation depend on protein synthesis in resting cells [Текст] / N. A. Setkov // Dokl. Akad. Nauk. - 1996. - Vol. 346, Is. 5. - P. 708-711. - Cited References: 15 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
C-FOS
   SYNTHESIS INHIBITORS
   3T3 CELLS
   TRANSCRIPTIONAL ACTIVATION
   DNA-SYNTHESIS
   FIBROBLASTS
   NUCLEI
   FUSION
   MYC
   JUN

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.

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Brief exposures of resting fibroblasts to okadaic acid stimulate DNA synthesis [Text] / N. A. Setkov, O. I. Epifanova // Cell Prolif. - 1997. - Vol. 30, Is. 1. - P. 7-19, DOI 10.1111/j.1365-2184.1997.tb00912.x. - Cited References: 32 . - ISSN 0960-7722

РУБ Cell Biology
Рубрики:
PDGF-INDUCED PROLIFERATION
   HAMSTER-EMBRYO CELLS
   PROTEIN-PHOSPHORYLATION
   PHOSPHATASE INHIBITORS
   MOUSE FIBROBLASTS
   3T3 CELLS
   FUSION
   TRANSFORMATION
   INDUCTION
   ARREST
Аннотация: To study further the factors providing for cellular quiescence, we used okadaic acid (OA) at concentrations (0.1, 1, 10 or 100 nM) inhibiting type 1 and/or type 2A protein phosphatases in mammalian cell cultures. Brief (2 h) exposure of resting (0.2% serum for 72 h) NIH 3T3 mouse fibroblasts to OA with subsequent incubation of cells in a medium with 0.2% serum, stimulated DNA synthesis at all concentrations studied. Maximal stimulation was observed following pre-incubation of resting cells with 10 nM OA. Treatment of cycling cells (10% serum) with OA (2 h pulses at 12 h intervals for 72 h) prevented their exit to the resting state on transfer to a medium with 0.2% serum. Brief exposures of resting cells to OA did not affect the rate of protein synthesis. OA pulses in the late pre-replicative period had no effect on the entry of serum-stimulated cells into the S phase. Cell fusion experiments with resting (serum-deprived) and proliferating (serum-stimulated) NIH 3T3 cells, using radioautography with a double-labelling technique, revealed that pre-incubation of resting cells with OA for 2 h before and after fusion abrogates their ability to suppress the onset of DNA synthesis in the nuclei of proliferating cells in heterodikaryons. The results indicate that protein phosphatases of type 1 and/or 2A may be involved in the growth-arrest machinery that provides for cellular quiescence.

WOS
Держатели документа:
VA ENGELHARDT MOL BIOL INST,MOSCOW 117984,RUSSIA
RUSSIAN ACAD SCI,INST BIOPHYS,SIBERIAN BRANCH,KRASNOYARSK,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.; Epifanova, O.I.

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