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PROTEIN-SYNTHESIS INHIBITORS, LIKE GROWTH-FACTORS, MAY RENDER RESTING 3T3 CELLS COMPETENT FOR DNA-SYNTHESIS - A AUTORADIOGRAPHIC AND CELL-FUSION STUDY [Text] / N. A. SETKOV [et al.] // Cell Prolif. - 1992. - Vol. 25, Is. 3. - P. 181-191, DOI 10.1111/j.1365-2184.1992.tb01393.x. - Cited References: 20 . - ISSN 0960-7722

РУБ Cell Biology
Рубрики:
C-MYC
   CYCLOHEXIMIDE
   FIBROBLASTS
   EXPRESSION
   INDUCTION
   GENES
   FOS
Аннотация: Serum-deprived (0.1-0.2%) resting NIH 3T3 mouse fibroblasts pre-incubated with cycloheximide (7.5-mu-g/ml), or puromycin (10-mu-g/ml), were fused with stimulated cells taken 10 h after changing the medium to one containing 10% serum, and DNA synthesis was investigated in the nuclei of monokaryons, homodikaryons and heterodikaryons using radioautography with the double-labelling technique. Pre-incubation of resting cells with inhibitors of protein synthesis for 1-4 h abolished their ability to suppress DNA synthesis in stimulated nuclei in heterokaryons. Three hours after the removal of cycloheximide from the medium, the resting cells acquired once again the inhibitory capacity for entry of stimulated nuclei into the S period. This inhibitory influence disappeared also in the case of post-fusion cycloheximide application as well as following an 8-12 h pre-treatment of resting cells with actinomycin D (1-mu-g/ml) prior to fusion. Pre-incubation of resting cells for 12 h with PDGF (1 u/ml-1) followed by an 8-48 h incubation in serum-free medium stimulated the onset of DNA synthesis. A brief exposure (45 min) of resting cells to cycloheximide (7.5-mu-g/ml), or puromycin (7.5-mu-g/ml), exerted a similar effect, inducing by itself the entry of cells into the S period. The results support the assumption that acquirement, by resting cells, of competence for DNA replication includes as a necessary step the down-regulation of intracellular growth inhibitors whose formation depends on protein synthesis.

WOS
Держатели документа:
ACAD SCI USSR,INST BIOPHYS,KRASNOYARSK,USSR
WA ENGELHARDT MOLEC BIOL INST,MOSCOW,USSR
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SETKOV, N.A.; KAZAKOV, V.N.; ROSENWALD, I.B.; MAKAROVA, G.F.; EPIFANOVA, O.I.

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Brief exposures of resting fibroblasts to okadaic acid stimulate DNA synthesis [Text] / N. A. Setkov, O. I. Epifanova // Cell Prolif. - 1997. - Vol. 30, Is. 1. - P. 7-19, DOI 10.1111/j.1365-2184.1997.tb00912.x. - Cited References: 32 . - ISSN 0960-7722

РУБ Cell Biology
Рубрики:
PDGF-INDUCED PROLIFERATION
   HAMSTER-EMBRYO CELLS
   PROTEIN-PHOSPHORYLATION
   PHOSPHATASE INHIBITORS
   MOUSE FIBROBLASTS
   3T3 CELLS
   FUSION
   TRANSFORMATION
   INDUCTION
   ARREST
Аннотация: To study further the factors providing for cellular quiescence, we used okadaic acid (OA) at concentrations (0.1, 1, 10 or 100 nM) inhibiting type 1 and/or type 2A protein phosphatases in mammalian cell cultures. Brief (2 h) exposure of resting (0.2% serum for 72 h) NIH 3T3 mouse fibroblasts to OA with subsequent incubation of cells in a medium with 0.2% serum, stimulated DNA synthesis at all concentrations studied. Maximal stimulation was observed following pre-incubation of resting cells with 10 nM OA. Treatment of cycling cells (10% serum) with OA (2 h pulses at 12 h intervals for 72 h) prevented their exit to the resting state on transfer to a medium with 0.2% serum. Brief exposures of resting cells to OA did not affect the rate of protein synthesis. OA pulses in the late pre-replicative period had no effect on the entry of serum-stimulated cells into the S phase. Cell fusion experiments with resting (serum-deprived) and proliferating (serum-stimulated) NIH 3T3 cells, using radioautography with a double-labelling technique, revealed that pre-incubation of resting cells with OA for 2 h before and after fusion abrogates their ability to suppress the onset of DNA synthesis in the nuclei of proliferating cells in heterodikaryons. The results indicate that protein phosphatases of type 1 and/or 2A may be involved in the growth-arrest machinery that provides for cellular quiescence.

WOS
Держатели документа:
VA ENGELHARDT MOL BIOL INST,MOSCOW 117984,RUSSIA
RUSSIAN ACAD SCI,INST BIOPHYS,SIBERIAN BRANCH,KRASNOYARSK,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.; Epifanova, O.I.

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The influence of food concentration and "water aggregation" on the reproductive parameters of Moina macrocopa (Crustacea : Cladocera) females [Текст] / E. S. Zadereev, V. G. Gubanov, I. V. Egorov // Zhurnal Obshchei Biol. - 1998. - Vol. 59, Is. 1. - P. 45-57. - Cited References: 27 . - ISSN 0044-4596

РУБ Biology
Рубрики:
DAPHNIA-MAGNA
   EGG SIZE
   DENSITY
   POPULATION
   INDUCTION
   PULEX
Аннотация: Combined influence of "water of aggregation" and food concentration on the reproductive parameters of Moina macrocopa females was investigated. In all experiments the influence of population density was associated with the effect of "water of aggregation" prepared earlier by keeping dense population (200 mu g/ml) of M. macrocopa. It was shown that life-span of M. macrocopa females in "water of aggregation" was not dependent on food concentration; under conditions of all tested food concentration it was less than in case of control clean water. There was no difference in proportion of males produced in "water of aggregation" and in control though more males were born under conditions of higher food concentration. In general "water of aggregation" causes a specific acceleration of female life history: the life-span and the period between clutches decrease, the amount of neonates in last clutches increases while the chance of male production at the beginning of reproduction period becames greater. The "water of aggregation" could also causes the formation of ephipial eggs instead of the first partenogenetic clutch. It seems that life strategy under conditions of very high mortality associated with "water of aggregation" is aimed to maximum realization of individual capacities in short period of time.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zadereev, E.S.; Gubanov, V.G.; Egorov, I.V.

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Fluorescence induced by light of 380-540 nm in leaves of cucumber depening on the vegetation time and irradiation regime [Текст] / V. V. Zavoruev, E. N. Zavorueva, A. V. Shelegov // Biofizika. - 2000. - Vol. 45, Is. 4. - P. 704-711. - Cited References: 25 . - ISSN 0006-3029

РУБ Biophysics
Рубрики:
CHLOROPHYLL
   INDUCTION
   RATIO
   INCREASE
Кл.слова (ненормированные):
slow fluorescence induction -- cucumber leaves -- vegetation time spectral conditions
Аннотация: The slow fluorescence induction produced iii cucumber leaves by light in the range of wavelengths 380-540 nm and intensity of 180 W/m(2) was studied. The ratio of fluorescence maxima in the red region (F(734)/F(682)) in young and mature leaves was approximately 2. It is assumed that this value depends on an increase in the contribution of the long-wavelength fluorescence due to the spillover effect. In plants grown under natural conditions, the paramster F(734)/F(682) correlated with the concentrations of chlorophyll and carotenoids. In plants grown hi the light of red and blue regions no such correlation was observed and the F(734)/F(682) remained unchanged. It is concluded that the F(534)/F(682) is affected by the intensity and spectral composition of exciting light used during the growing.

WOS
Держатели документа:
Russian Acad Sci, Inst Computat Modeling, Siberian Div, Krasnoyarsk 660036, Russia
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zavoruev, V.V.; Zavorueva, E.N.; Shelegov, A.V.

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Chemical composition of water conditioned by crowded Moina macracopa (Crustacea : Cladocera) [Text] / G. S. Kalacheva [et al.] // Russ. J. Ecol. - 2000. - Vol. 31, Is. 2. - P. 113-117, DOI 10.1007/BF02828365. - Cited References: 25 . - ISSN 1067-4136

РУБ Ecology
Рубрики:
DAPHNIA-PULEX
   ZOOPLANKTON
   PARAMETERS
   INDUCTION
Кл.слова (ненормированные):
Cladocera -- metabolic products -- chemical analysis -- chemical composition
Аннотация: The chemical composition of water conditioned by colonies of Moina macrocopa and involved in the regulation of their life cycle was analyzed in comparison with water samples from natural water bodies. Dechlorinated tap water was used as the control medium. The results showed that metabolism of crustaceans leads to an increase in the concentrations of phosphorus and nitrogen in all forms, the appearance of protein and carbohydrate fractions, and changes in the composition of lipid fractions in the water conditioned by their colonies. The contents of all organic components are very low, which makes their identification difficult. Concentrations of substances identified in the conditioned water do not exceed those occurring in nature.

WOS
Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zadereev, E.S.; Gubanov, V.G.; Gribovskaya, I.V.

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Assessment of the Volatility and Thermal Stability of Chemicals that Stimulate Females of Moina macrocopa (Cladocera) to Produce Diapausing Eggs [Text] / T. S. Lopatina, E. S. Zadereev // Russ. J. Ecol. - 2015. - Vol. 46, Is. 1. - P103-108, DOI 10.1134/S1067413615010105. - Cited References:25 . - ISSN 1067-4136. - ISSN 1608-3334

РУБ Ecology
Рубрики:
SEXUAL REPRODUCTION
   INDUCTION
   DAPHNIA
   METABOLITES
   CRUSTACEA
Кл.слова (ненормированные):
cladocerans -- diapause -- chemical communication -- chemical cues
Аннотация: The volatility and thermal stability of chemicals that are contained in the metabolic products of Moina macrocopa females that stimulate the production of diapausing eggs in these females have been studied in experiments with separately cultivated individuals. It has been shown that the gametogenesis-stimulating chemicals in this species are nonvolatile compounds: the effect of the medium that contains the metabolic products of the organism was not decreased after many hours of blowing with argon. Prolonged heating up to 80 degrees C also did not deactivate the ability of these products to change the reproductive mode. The effect of the medium that contains these products decreased after boiling, but if the possibilities of their oxidation or binding with salts of metals were excluded, they retained the ability to change the reproductive mode of the animals even after boiling.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Lopatina, T.S.; Zadereev, E.S.

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Extraction of info chemicals inducing the production of resting eggs in cladocerans [Text] / E. S. Zadereev, T. S. Lopatina // Dokl. Biochem. Biophys. - 2015. - Vol. 461, Is. 1. - P127-130, DOI 10.1134/S1607672915020192. - Cited References:13 . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
MOINA-MACROCOPA CLADOCERA
   REPRODUCTION
   CRUSTACEANS
   INDUCTION
   CUES

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.

Доп.точки доступа:
Zadereev, E. S.; Lopatina, T. S.

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Effect of light intensity on the age dependence of nonphotochemical fluorescence quenching in wheat leaf [Text] / T. V. Nesterenko, V. N. Shikhov, A. A. Tikhomirov // Photosynthetica. - 2015. - Vol. 53, Is. 4. - P617-620, DOI 10.1007/s11099-015-0133-2. - Cited References:16. - The study was performed within the framework of subject No. 56.1.4., Section VI in accordance with State Program of RAS Fundamental Research for 2013-2020. . - ISSN 0300-3604. - ISSN 1573-9058

РУБ Plant Sciences
Рубрики:
CHLOROPHYLL FLUORESCENCE
   PHOTOSYNTHESIS
   LEAVES
   INDUCTION
   ENERGY
Кл.слова (ненормированные):
chlorophyll fluorescence induction -- leaf age -- photosystem II
Аннотация: The effects of actinic light (AL) intensity on the age dependence of nonphotochemical fluorescence quenching (q(N)) and effective quantum yield in PSII (I broken vertical bar(PSII)) were studied in continuously illuminated wheat leaves of the upper tier. Regular changes were revealed in both age dependence of q(N) at elevated AL intensities and light curves of q(N). These changes are related to alterations in strategies of redistribution and use of absorbed light energy by the photosynthetic apparatus at different stages of wheat leaf development. Unlike I broken vertical bar(PSII), q(N) as a parameter was more sensitive to the differences in the leaf age at a certain range of light intensities. At the same time, the stability of q(N) at moderate light intensities may serve as an indication of leaf maturity.

WOS,
Scopus
Держатели документа:
RAS, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Nesterenko, T. V.; Shikhov, V. N.; Tikhomirov, A. A.; RAS [56.1.4]

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