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Plants with genetically encoded autoluminescence / T. Mitiouchkina, A. S. Mishin, L. G. Somermeyer [et al.] // Nat. Biotechnol. - 2020, DOI 10.1038/s41587-020-0500-9. - Cited References:17. - This study was designed, performed and funded by Planta LLC. We thank K. Wood for assisting in manuscript development. Planta acknowledges support from the Skolkovo Innovation Centre. We thank D. Bolotin and the Milaboratory (milaboratory. com) for access to computing and storage infrastructure. We thank S. Shakhov for providing photography equipment. The Synthetic Biology Group is funded by the MRC London Institute of Medical Sciences (UKRI MC-A658-5QEA0, K.S.S.). K.S.S. is supported by an Imperial College Research Fellowship. Experiments were partially carried out using equipment provided by the Institute of Bioorganic Chemistry of the Russian Academy of Sciences.ore Facility (CKP IBCH; supported by the Russian Ministry of Education and Science Grant RFMEFI62117X0018). The F.A.K. lab is supported by ERC grant agreement 771209-CharFL. This project received funding from the European Union's Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie Grant Agreement 665385. K.S.S. acknowledges support by President's Grant 075-15-2019-411. Design and assembly of some of the plasmids was supported by Russian Science Foundation grant 19-74-10102. Imaging experiments were partially supported by Russian Science Foundation grant 17-14-01169p. LC-MS/MS analyses of extracts were supported by Russian Science Foundation grant 16-14-00052p. Design and assembly of plasmids was partially supported by grant 075-15-2019-1789 from the Ministry of Science and Higher Education of the Russian Federation allocated to the Center for Precision Genome Editing and Genetic Technologies for Biomedicine.
. - Article in press. - ISSN 1087-0156. - ISSN 1546-1696РУБ Biotechnology & Applied Microbiology
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Аннотация: Autoluminescent plants engineered to express a bacterial bioluminescence gene cluster in plastids have not been widely adopted because of low light output. We engineered tobacco plants with a fungal bioluminescence system that converts caffeic acid (present in all plants) into luciferin and report self-sustained luminescence that is visible to the naked eye. Our findings could underpin development of a suite of imaging tools for plants.
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Держатели документа:
Planta LLC, Moscow, Russia.
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia.
IST Austria, Klosterneuburg, Austria.
Pirogov Russian Natl Res Med Univ, Moscow, Russia.
Russian Acad Sci, Siberian Branch, Inst Biophys Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.
Aivok LLC, Moscow, Russia.
Bot Garden Lomonosov Moscow State Univ, Moscow, Russia.
MRC, Synthet Biol Grp, London Inst Med Sci, London, England.
Imperial Coll London, Inst Clin Sci, Fac Med, London, England.
Imperial Coll London, Imperial Coll Ctr Synthet Biol, London, England.
Доп.точки доступа:
Mitiouchkina, Tatiana; Mishin, Alexander S.; Somermeyer, Louisa Gonzalez; Markina, Nadezhda M.; Chepurnyh, Tatiana, V; Guglya, Elena B.; Karataeva, Tatiana A.; Palkina, Kseniia A.; Shakhova, Ekaterina S.; Fakhranurova, Liliia, I; Chekova, Sofia, V; Tsarkova, Aleksandra S.; Golubev, Yaroslav, V; Negrebetsky, Vadim V.; Dolgushin, Sergey A.; Shalaev, Pavel, V; Shlykov, Dmitry; Melnik, Olesya A.; Shipunova, Victoria O.; Deyev, Sergey M.; Bubyrev, Andrey, I; Pushin, Alexander S.; Choob, Vladimir V.; Dolgov, Sergey, V; Kondrashov, Fyodor A.; Yampolsky, Ilia, V; Sarkisyan, Karen S.; Tsarkova, Aleksandra; Planta LLC; Skolkovo Innovation Centre; MRC London Institute of Medical Sciences [UKRI MC-A658-5QEA0]; Imperial College Research Fellowship; Institute of Bioorganic Chemistry of the Russian Academy of Sciences.ore Facility (CKP IBCH - Russian Ministry of Education and Science Grant) [RFMEFI62117X0018]; ERC grant [771209-CharFL]; European Union's Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie Grant [665385]; Russian Science Foundation grantRussian Science Foundation (RSF) [19-74-10102, 17-14-01169p, 16-14-00052p]; Ministry of Science and Higher Education of the Russian Federation [075-15-2019-1789]; [075-15-2019-411]
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