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1.


   
    Biosynthesis of multi-component polyhydroxyalkanoates by the bacterium Wautersia eutropha [Text] / T. G. Volova, G. S. Kalacheva, A. . Steinbuchel // Macromol. Symp. - 2008. - Vol. 269: 4th European Symposium on Biopolymers (OCT 02-04, 2007, Kusadasi, TURKEY). - P1-7, DOI 10.1002/masy.200850901. - Cited References: 20 . - 7. - ISSN 1022-1360
РУБ Polymer Science
Рубрики:
RALSTONIA-EUTROPHA
   ALCALIGENES-EUTROPHUS
   AEROMONAS-HYDROPHILA
   ENHANCED PRODUCTION
   PHA SYNTHASE
   COPOLYESTERS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE)
   3-HYDROXYBUTYRATE
   ACID
Кл.слова (ненормированные):
autotrophic and mixotrophic growth -- multi-component polyhydroxyalkanoates -- Wautersia eutropha
Аннотация: The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild type strains (H16 and 85786), it has been first found that under mixotrophic growth conditions -CO2+ co-substrate (alkanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chain-length monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate as major monomers and hydroxyhexanoate, hydroxyheptanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs were synthesized and their physicochemical properties examined.

Держатели документа:
[Volova, Tatiana G.
Kalacheva, Galina S.] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
[Volova, Tatiana G.] Siberian Fed Univ, Krasnoyarsk, Russia
[Steinbuechel, Alexander] Inst Mol Microbiol & Biotechnol, Munster, Germany : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Steinbuchel, A...

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2.


   
    A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs [Text] / T. . Volova [et al.] // PLoS One. - 2014. - Vol. 9, Is. 2. - Ст. e87551, DOI 10.1371/journal.pone.0087551. - Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1932-6203
РУБ Multidisciplinary Sciences
Рубрики:
RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYHYDROXYALKANOATES
   AEROMONAS-HYDROPHILA
   ESCHERICHIA-COLI
   MOLECULAR-WEIGHT
   SURFACE-ENERGY
   NORTH PACIFIC
   TERPOLYESTER
   BIOSYNTHESIS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

WOS
Держатели документа:
[Volova, Tatiana
Kiselev, Evgeniy
Nikolaeva, Elena
Sukovatiy, Aleksey
Shishatskaya, Ekaterina] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Volova, Tatiana
Vinogradova, Olga
Shishatskaya, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Chistyakov, Anton] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Kiselev, E...; Vinogradova, O...; Nikolaeva, E...; Chistyakov, A...; Sukovatiy, A...; Shishatskaya, E...; Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]

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3.


   
    Fatty acid composition and polyhydroxyalkanoates production by Cupriavidus eutrophus B-10646 cells grown on different carbon sources [Text] / N. Zhila, G. Kalacheva, T. Volova // Process Biochem. - 2015. - Vol. 50, Is. 1. - P69-78, DOI 10.1016/j.procbio.2014.10.018. - Cited References:66. - This study was supported by the Russian Science Foundation (Grant No.14-26-00039). . - ISSN 1359-5113. - ISSN 1873-3298
РУБ Biochemistry & Molecular Biology + Biotechnology & Applied Microbiology
Рубрики:
HYDROGEN-OXIDIZING BACTERIA
   CHAIN TRANSFER AGENTS
   RALSTONIA-EUTROPHA
Кл.слова (ненормированные):
Cupriavidus eutrophus -- Fatty acid -- Polyhydroxyalkanoates -- Molecular -- weight -- Different substrates
Аннотация: Growth of Cupriavidus eutrophus B-10646, fatty acid (FA) composition of the lipids in the cytoplasmic membrane and cell wall, and polyhydroxyalkanoate (PHA) accumulation by the cells of this new promising producer of PHAs were studied in batch culture, using various carbon sources (fructose, glucose, oleic acid, sunflower seed oil, glycerol, CO2:O-2:H-2). In cells grown on sugars and in autotrophic culture, during the phase of active cell growth, the major FAs of the lipids of cytoplasmic membrane (CMLs) were palmitic, palmitoleic, and cis-vaccenic acids. In the stationary phase of cultivation, cyclopropane FAs were also detected. When carbohydrate substrate was replaced by oleic acid or sunflower seed oil, the proportion of oleic acid in the total FAs of the cytoplasmic membrane increased considerably. In addition to that, the lipid FAs of bacterial cells grown on sunflower seed oil also contained linoleic acid, which is the major acid of sunflower seed oil. The major acids of strongly bound lipids of cell wall (SBLs) were myristic acid and long-chain-length beta-hydroxy acids. Molecular weight of the polymer was measured during its accumulation in cells; for the first time, the presence of 2 polymer fractions (high-molecular weight and low-molecular-weight ones) was recorded in cells with a low polymer content. (C) 2014 Elsevier Ltd. All rights reserved.

WOS
Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Zhila, Natalia; Kalacheva, Galina; Volova, Tatiana; Russian Science Foundation [14-26-00039]

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4.


   
    Synthesis of poly(3-hydroxybutyrate) by the autotrophic CO-oxidizing bacterium Seliberia carboxydohydrogena Z-1062 [Text] / T. Volova, N. Zhila, E. Shishatskaya // J. Ind. Microbiol. Biotechnol. - 2015. - Vol. 42, Is. 10. - P1377-1387, DOI 10.1007/s10295-015-1659-9. - Cited References:36. - The research was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505). . - ISSN 1367-5435. - ISSN 1476-5535
РУБ Biotechnology & Applied Microbiology
Рубрики:
CARBON-MONOXIDE
   RALSTONIA-EUTROPHA
   POLYHYDROXYALKANOATES
   GROWTH
   H-2
Кл.слова (ненормированные):
Carboxydobacteria -- Synthesis -- Poly(3-hydroxybutyrate) -- Carbon monoxide
Аннотация: The present study addresses growth parameters and physiological and biochemical characteristics of the aerobic CO-oxidizing carboxydobacterium Seliberia carboxydohydrogena Z-1062. Poly(3-hydroxybutyrate) yields were investigated in experiments with limiting concentrations of mineral nutrients (nitrogen or sulfur or nitrogen and sulfur) in batch culture of S. carboxydohydrogena Z-1062 grown on gas mixtures consisting of CO2, O-2, H-2, and CO. CO concentrations of 10, 20, and 30 % v/v did not affect polymer synthesis, whose content after 56-h cultivation under limiting concentrations of nitrogen and sulfur was 52.6-62.8 % of biomass weight at a productivity of 0.13-0.22 g/L h. The inhibitory effect of CO on cell concentration was revealed at CO concentration of 30 % v/v. That also caused a decrease in substrate (H-2 and O-2) use efficiency. Thus, this carboxydobacterium can be regarded as a potential producer of polyhydroxyalkanoates from industrial hydrogenous sources.

WOS,
Scopus
Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Volova, Tatiana; Zhila, Natalia; Shishatskaya, Ekaterina; Russian Academy of Sciences [01201351505]

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5.


   
    Polymer Films of Poly-3-hydroxybutyrate Synthesized byCupriavidus necatorfrom Different Carbon Sources / E. Shishatskaya, I. Nemtsev, A. Lukyanenko [et al.] // J. Polym. Environ. - 2020, DOI 10.1007/s10924-020-01924-3. - Cited References:54. - The reported study was funded by RFBR and KKRF Grant No. 19-43-240012 "Biological and physical principles of production of new generation biomaterials". The work was carried out as part of the State Assignment of the Ministry of Education and Science of the Russian Federation No. FSRZ-2020-0006. The authors would like to express their special thanks to Krasnoyarsk Regional Center of Research Equipment of Federal Research Center "Krasnoyarsk Science Center SB RAS" for providing equipment to ensure the accomplishment of this project. . - Article in press. - ISSN 1566-2543. - ISSN 1572-8919
РУБ Engineering, Environmental + Polymer Science
Рубрики:
CHEMOMECHANICAL PROPERTIES
   RALSTONIA-EUTROPHA
   SURFACE-ROUGHNESS
Кл.слова (ненормированные):
Degradable P(3HB) -- Various carbon substrates -- Films -- Structure -- Properties
Аннотация: Films were prepared from 2% solutions of biodegradable poly-3-hydroxybutyrate [P(3HB)] and investigated. The polymer was synthesized by theCupriavidus necatorB-10646 bacterium cultivated using various carbon sources (glucose and glycerol of different degrees of purity, containing 0.3 to 17.93% impurities). Glycerol as the substrate influenced molecular-weight properties and crystallinity of the polymer without affecting its temperature characteristics. The P(3HB) specimens synthesized from glycerol had reduced M-w(300-400 kDa) and degree of crystallinity (50-55%) compared to the specimens synthesized from glucose (860 kDa and 76%, respectively). The low-crystallinity P(3HB) specimens, regardless of the degree of purity of glycerol, produced a beneficial effect on the properties of polymer films, which had a better developed folded surface and increased hydrophilicity. The values of the highest roughness (R-a) of the films synthesized from glycerol were 1.8 to 4.0 times lower and the water angles 1.4-1.6 times smaller compared to the films synthesized from glucose (71.75 nm and 87.4 degrees, respectively). Those films performed better as cell scaffolds: the number of viable NIH fibroblasts was 1.7-1.9 times higher than on polystyrene (control) or films of P(3HB) synthesized from glucose.

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Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys, Krasnoyarsk, Russia.
RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, LV Kirenskii Inst Phys, Krasnoyarsk, Russia.

Доп.точки доступа:
Shishatskaya, Ekaterina; Nemtsev, Ivan; Lukyanenko, Anna; Vasiliev, Alexander; Kiselev, Evgeniy; Sukovatyi, Aleksey; Volova, Tatiana; RFBRRussian Foundation for Basic Research (RFBR); KKRF [19-43-240012]; Ministry of Education and Science of the Russian FederationMinistry of Education and Science, Russian Federation [FSRZ-2020-0006]

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6.


   
    Properties of degradable polyhydroxyalkanoates with different monomer compositions / T. Volova, E. Kiselev, I. Nemtsev [et al.] // Int. J. Biol. Macromol. - 2021. - Vol. 182. - P98-114, DOI 10.1016/j.ijbiomac.2021.04.008. - Cited References:106. - The part of the reported study on polymer synthesis and examinationwas funded by RFBR and KKRF [Grant No. 19-43-240012 "Biological and physical principles of production of new generation biomaterials"]. The work on production and investigation of polymer films was carried out as part of the State Assignment of the Ministry of Education and Science of the Russian Federation [Grant No. FSRZ-2020-0006]. . - ISSN 0141-8130. - ISSN 1879-0003
РУБ Biochemistry & Molecular Biology + Chemistry, Applied + Polymer Science
Рубрики:
PALM KERNEL OIL
   RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYMERS
Кл.слова (ненормированные):
Polyhydroxyalkanoates -- Chemical composition -- Physicochemical properties -- Films -- Microstructure -- Surface properties
Аннотация: Purpose: To synthesize and investigate polyhydroxyalkanoates (PHAs) with different monomer composition and percentages and polymer films prepared from them. Results: Various PHAs: homopolymer poly-3-hydroxybutyrate P(3HB) and 2-, 3-, and 4-component copolymers comprising various combinations of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), and 3-hydroxyhexanoate (3HHx) monomers were synthesized under specialized conditions. Relationships were found between the monomer composition of PHAs and their molecular-weight and thermal properties and degree of crystallinity. All copolymers had decreased weight average molecular weights, Mw (to 390-600 kDa), and increased values of polydispersity (3.2-4.6) compared to the P(3HB). PHA copolymers showed different thermal behavior: an insignificant decrease in Tmelt and the presence of the second peak in the melting region and changes in parameters of crystallization and glass transition. At the same time, they retained thermostability, and the difference between Tmelt and Tdegr was at least 100-120 degrees C. Incorporation of 4HB, 3HV, and 3HHx monomer units into the 3-hydroxybutyrate chain caused changes in the amorphous to crystalline ratio and decreased the degree of crystallinity (Cx) to 20-40%. According to the degree to which the monomers reduced crystallinity, they were ranked as follows: 4HB - 3HHx - 3HV. A unique set of films was produced; their surface properties and physical/mechanical properties were studied as dependent on PHA composition; monomers other than 3hydroxybutyrate were found to enhance hydrophilicity, surface development, and elasticity of polymer films. Conclusion: An innovative set of PHA copolymers was synthesized and solution-cast films were prepared from them; the copolymers and films were investigated as dependent on polymer chemical composition. Results obtained in the present study contribute to the solution of a critical issue of producing degradable polymer materials. (C) 2021 Elsevier B.V. All rights reserved.

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Держатели документа:
Siberian Fed Univ, 79 Svohodnyi Av, Krasnoyarsk 660041, Russia.
SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Inst Biophys, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, LV Kirensky Inst Phys, 50-12 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, T.; Kiselev, E.; Nemtsev, I.; Lukyanenko, A.; Sukovatyi, A.; Kuzmin, A.; Ryltseva, G.; Shishatskaya, E.; Petrovich, Kuzmin Andrey; RFBRRussian Foundation for Basic Research (RFBR) [19-43-240012]; Ministry of Education and Science of the Russian FederationMinistry of Education and Science, Russian Federation [FSRZ-2020-0006]

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7.


   
    Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources / N. O. Zhila, K. Y. Sapozhnikova, E. G. Kiselev [et al.] // Polymers. - 2021. - Vol. 13, Is. 18. - Ст. 3142, DOI 10.3390/polym13183142. - Cited References:78. - This work was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 075-15-2021-626) in accordance with Resolution No 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (strain isolation, polymer synthesis and investigation), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (study of film properties). . - ISSN 2073-4360
РУБ Polymer Science
Рубрики:
RALSTONIA-EUTROPHA
   POLY(3-HYDROXYBUTYRATE) PRODUCTION
   PLANT OIL
   ACID
Кл.слова (ненормированные):
Cupriavidus necator IBP -- SFU-1 -- cell growth and PHA synthesis -- various -- carbon sources -- PHA composition and properties -- polymer films
Аннотация: The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7-8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 +/- 15 kDa) and weight-average (850 +/- 25 kDa) molecular weights and the lowest polydispersity (2.9 +/- 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.

WOS
Держатели документа:
Siberian Fed Univ, Basic Dept Biotechnol, Sch Fundamental Biol & Biotechnol, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia.
SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, LV Kirensky Inst Phys, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zhila, Natalia O.; Sapozhnikova, Kristina Yu; Kiselev, Evgeniy G.; Vasiliev, Alexander D.; Nemtsev, Ivan, V; Shishatskaya, Ekaterina, I; Volova, Tatiana G.; Russian FederationRussian Federation [075-15-2021-626, 220]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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8.


   
    Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass / T. G. Volova, E. G. Kiselev, A. V. Demidenko [et al.] // Polymers. - 2022. - Vol. 14, Is. 1. - Ст. 132, DOI 10.3390/polym14010132. - Cited References:93. - This study was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No. 075-15-2021-626) in accordance with Resolution No. 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis, properties), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (films production, surface properties). . - ISSN 2073-4360
РУБ Polymer Science
Рубрики:
GLUCOSE-UTILIZING STRAIN
   RALSTONIA-EUTROPHA
   ASPERGILLUS-NIGER
   ACID
Кл.слова (ненормированные):
Jerusalem artichoke hydrolysates -- PHA synthesis -- productivity -- polyhydroxyalkanoates
Аннотация: One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains-C. necator B-10646 and R. eutropha B 5786 and B 8562-were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 degrees C followed by acid hydrolysis at 60 degrees C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100-120 degrees C difference between the T-melt and T-degr, prevailing of the crystalline phase over the amorphous one (C-x between 69 and 75%), and variations in weight average molecular weight (409-480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and epsilon-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology-finding widely available and inexpensive substrates.

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Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Basic Dept Biotechnol, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys, SB RAS, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, LV Kirensky Phys Inst, Fed Res Ctr, SB RAS, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Volova, Tatiana G.; Kiselev, Evgeniy G.; Demidenko, Alexey V.; Zhila, Natalia O.; Nemtsev, Ivan V.; Lukyanenko, Anna V.; Kiselev, Evgeniy; Project "Agropreparations of the new generation: a strategy of construction and realization" [075-15-2021-626]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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