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A model of bacterial cell cycle duration based on DnaA dynamics and estimation of the population cost of bacterial plasmids [Text] / A. N. Shuvaev, A. V. Brilkov // Dokl. Biochem. Biophys. - 2007. - Vol. 416, Is. 01.06.2013. - P233-236, DOI 10.1134/S1607672907050018. - Cited References: 17 . - ISSN 1607-6729

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
ESCHERICHIA-COLI
   GROWTH-RATE
   PARAMETERS
   SIZE
   TIME

Держатели документа:
[Shuvaev, A. N.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Brilkov, A. V.] Krasnoyarsk Fed Univ, Krasnoyarsk 660062, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shuvaev, A.N.; Brilkov, A.V.

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In vitro and transdermal penetration of PHBV micro/nanoparticles [Text] / G. . Eke [et al.] // J. Mater. Sci.-Mater. Med. - 2014. - Vol. 25, Is. 6. - P1471-1481, DOI 10.1007/s10856-014-5169-5. - Cited References: 38. - The study was supported by the Government of the Russian Federation (Decree No. 220 of 09.04.2010) (Agreement No. 11.G34.31.0013) and (Grant No MD-3112.2012.4). We gratefully acknowledge the EC FP7 SKINTREAT project and the State Planning Organization (Turkey) for the grant to establish BIOMATEN. Mr. A. Buyuksungur is acknowledged for his contributions with CLSM. . - ISSN 0957-4530. - ISSN 1573-4838

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DRUG-DELIVERY
   PLGA NANOPARTICLES
   CELLULAR UPTAKE
   MICROPARTICLES
   POLYHYDROXYALKANOATES
   CYTOTOXICITY
   SIZE
   POLYESTERS
   MECHANISM
   CELLS
Аннотация: The purpose of this study was to develop micro and nano sized drug carriers from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and study the cell and skin penetration of these particles. PHBV micro/nanospheres were prepared by o/w emulsion method and were stained with a fluorescent dye, Nile Red. The particles were fractionated by centrifugation to produce different sized populations. Topography was studied by SEM and average particle size and its distribution were determined with particle sizer. Cell viability assay (MTT) was carried out using L929 fibroblastic cell line, and particle penetration into the cells were studied. Transdermal permeation of PHBV micro/nanospheres and tissue reaction were studied using a BALB/c mouse model. Skin response was evaluated histologically and amount of PHBV in skin was determined by gas chromatography-mass spectrometry. The average diameters of the PHBV micro/nanosphere batches were found to be 1.9 mu m, 426 and 166 nm. Polydispersity indices showed that the size distribution of micro sized particles was broader than the smaller ones. In vitro studies showed that the cells had a normal growth trend. MTT showed no signs of particle toxicity. The 426 and 166 nm sized PHBV spheres were seen to penetrate the cell membrane. The histological sections revealed no adverse effects. In view of this data nano and micro sized PHBV particles appeared to have potential to serve as topical and transdermal drug delivery carriers for use on aged or damaged skin or in cases of skin diseases such as psoriasis, and may even be used in gene transfer to cells.

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Держатели документа:
[Eke, G.
Hasirci, N.
Hasirci, V.] Middle E Tech Univ, Dept Micro & Nanotechnol, TR-06800 Ankara, Turkey
[Eke, G.
Hasirci, N.
Hasirci, V.] METU Ctr Excellence Biomat & Tissue Engn, BIOMATEN, TR-06800 Ankara, Turkey
[Eke, G.] Ahi Evran Univ, Fac Arts & Sci, Dept Chem, TR-40100 Kirsehir, Turkey
[Kuzmina, A. M.
Shishatskaya, E. I.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Goreva, A. V.
Shishatskaya, E. I.] Inst Biophys SB RAS, Krasnoyarsk 66003, Russia
[Hasirci, N.
Hasirci, V.] Middle E Tech Univ, Dept Biotechnol, TR-06800 Ankara, Turkey
[Hasirci, N.
Hasirci, V.] Middle E Tech Univ, Dept Biomed Engn, TR-06800 Ankara, Turkey
[Hasirci, N.] Middle E Tech Univ, Dept Chem, TR-06800 Ankara, Turkey
[Hasirci, V.] Middle E Tech Univ, Dept Biol Sci, TR-06800 Ankara, Turkey
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Eke, G...; Kuzmina, A.M.; Goreva, A.V.; Shishatskaya, E.I.; Hasirci, N...; Hasirci, V...; Government of the Russian Federation [220, 11.G34.31.0013, MD-3112.2012.4]; EC FP7 SKINTREAT project; State Planning Organization (Turkey)

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Detonation Nanodiamond-Assisted Carbon Nanotube Growth by Hot Filament Chemical Vapor Deposition / I. P. Kudarenko [et al.] // Phys. Status Solidi B-Basic Solid State Phys. - 2018. - Vol. 255, Is. 1. - Ст. 1700286, DOI 10.1002/pssb.201700286. - Cited References:28. - The work was supported by RSF project 17-72-10173. . - ISSN 0370-1972. - ISSN 1521-3951

РУБ Physics, Condensed Matter
Рубрики:
DIAMOND
   FILMS
   HFCVD
   FABRICATION
   GRAPHITE
   SCIENCE
   SIZE
   CVD
Кл.слова (ненормированные):
carbon nanotubes -- catalytic growth -- diamond -- hot filament chemical vapor -- deposition -- nanomaterials -- synthesis
Аннотация: Substrates pretreatment in suspensions of a detonation nanodiamond is widely used for nucleation of diamond growth by chemical vapor deposition (CVD). We found that iron inclusions in the nanodiamond provide catalytical growth of carbon nanotubes during CVD in a hot filament reactor (HF CVD). Carbon nanotubes grow in the area between two adjacent Si wafers. The diameters of such obtained nanotubes were in the range of 10-100 nm and the length of the tubes reaches about 10 mu m. The proposed HF CVD method has convincing potential for the fabrication of carbon nanotube coatings on a large surface area.

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Держатели документа:
Moscow MV Lomonosov State Univ, Dept Phys, Moscow 119991, Russia.
Univ Eastern Finland, Dept Phys & Math, Joensuu 80101, Finland.
RAS, Fed Sci Res Ctr Crystallog & Photon, AV Shubnikov Inst Crystallog, Moscow 119333, Russia.
Natl Res Ctr, Kurchatov Inst, Moscow 123182, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Kudarenko, Ilya P.; Malykhin, Sergei A.; Orekhov, Andrey S.; Puzyr, Aleksey P.; Kleshch, Victor I.; Ismagilov, Rinat R.; Obraztsov, Alexander N.; RSF [17-72-10173]

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Comparative Assessment of the Content of Transition Metals (Cu, Zn, Mn, Pb, and Cd) and Radiocesium (Cs-137) in Pike (Esox lucius) and Burbot (Lota lota) of the Yenisei River / T. A. Zotina, O. V. Anishchenko, E. A. Trofimova, D. V. Dementiev // Contemp. Probl. Ecol. - 2022. - Vol. 15, Is. 1. - P91-99, DOI 10.1134/S1995425522010115. - Cited References:36. - This work was supported by the Russian Foundation for Basic Research, grant no. 18-44-240003, and the Government of Krasnoyarsk krai, together with the Krasnoyarsk Regional Fund for Support of Scientific and Technical Activity, grant no. 20-44-240004. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
FRESH-WATER FISH
   NORTHERN PIKE
   HEAVY-METALS
   BRAMA L.
   FOOD-WEB
   SIZE
Кл.слова (ненормированные):
piscivorous fish -- toxic metals -- body length -- body weight -- size effect
Аннотация: The trophic position of fish is one of the most important factors controlling the accumulation of potentially toxic elements and compounds in fish tissues, primarily via the spectrum of fish nutrition. In this study, the content of potentially toxic transition metals (Cu, Zn, Mn, Pb, and Cd) and radiocesium (Cs-137) in the edible tissues (muscles and liver) of two representatives of the fish-eating ichthyofauna of the Yenisei River, northern pike (Esox lucius) and burbot (Lota lota), have been comparatively studied relatively to the size of the fish. A significant decrease in the content of radiocesium and zinc in the muscles of pike and zinc in the liver of pike with an increase in body size has been recorded in juvenile pikes with a body weight (W) of less than 0.35 kg. For larger sexually mature pikes, no significant size dependences of the accumulation of metals in tissues are found. A positive correlation is found between the content of Cs-137, Cu, Zn, and Mn in muscles and the size of burbot in the W range from 0.42 to 1.62 kg. The tissues of burbots and pikes of the same size significantly (p < 0.05) differ in the concentration of metals in their tissues: concentrations of Zn and Mn are 1.6-2.2 times higher in the muscle of burbot; the concentration of Pb is 1.8 times higher in muscle of pike; Cu is twice as high in the liver of burbot; and Zn and Mn are 4.7 and 1.6 times higher in the liver of pike, respectively. These differences may be due to the different food spectra of pike and burbot. Concentrations of Cu, Zn, and Mn in the liver of pike are 3-7 times higher than in muscle; the concentration of Cu in the liver of burbot is 5 times higher than in muscle. Pb and Cd tend to be higher in liver than muscle for both fish species. Despite the revealed size dependences, the concentrations of potentially toxic metals and radiocesium in the muscles and liver of fish are below the permissible concentrations for food. These results can be used to assess environmental risks for the population consuming fish, as well as to plan for the long-term environmental monitoring of rivers using representatives of piscivorous fish.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, 50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zotina, T. A.; Anishchenko, O., V; Trofimova, E. A.; Dementiev, D., V; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-44-240003]; Government of Krasnoyarsk krai; Krasnoyarsk Regional Fund for Support of Scientific and Technical Activity [20-44-240004]

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