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Use of proZZ-obelin fusion protein in bioluminescent immunoassay [Text] / L. A. Frank, V. A. Illarionova, E. S. Vysotski // Biochem. Biophys. Res. Commun. - 1996. - Vol. 219, Is. 2. - P475-479, DOI 10.1006/bbrc.1996.0258. - Cited References: 21 . - 5. - ISSN 0006-291X

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
ESCHERICHIA-COLI
   EXPRESSION
   AEQUORIN
   PURIFICATION
   SYSTEM
Аннотация: Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coil by recombinant DNA techniques. The pro2Z-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 x 10(15) photons per mg of protein. (C) 1996 Academic Press, Inc.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.; Illarionova, V.A.; Vysotski, E.S.

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NMR-derived Topology of a GFP-photoprotein Energy Transfer Complex [Text] / M. S. Titushin [et al.] // J. Biol. Chem. - 2010. - Vol. 285, Is. 52. - P40891-40900, DOI 10.1074/jbc.M110.133843. - Cited References: 54. - This work was supported by the National Natural Science Foundation of China, Ministry of Science and Technology of China, CAS Research Grant, CAS Fellowship for Young International Scientists Grant, Russian Foundation for Basic Research (08-09-92209 RFBR-China joint grant), SB RAS Grant 2, "Molecular and Cell Biology" program of RAS, Bayer AG (Germany), and by the University of Georgia Research Foundation and the Georgia Research Alliance. . - ISSN 0021-9258

РУБ Biochemistry & Molecular Biology
Рубрики:
GREEN-FLUORESCENT PROTEIN
   STRUCTURAL DETERMINANTS
   RENILLA BIOLUMINESCENCE
   ANGSTROM RESOLUTION
   CRYSTAL-STRUCTURE
   ELECTRON-DENSITY
   SOFTWARE
   PROGRAM
   BINDING
   SYSTEM
Аннотация: Forster resonance energy transfer within a protein-protein complex has previously been invoked to explain emission spectral modulation observed in several bioluminescence systems. Here we present a spatial structure of a complex of the Ca2+ regulated photoprotein clytin with its green-fluorescent protein (cgGFP) from the jellyfish Clytia gregaria, and show that it accounts for the bioluminescence properties of this system in vitro. We adopted an indirect approach of combining x-ray crystallography determined structures of the separate proteins, NMR spectroscopy, computational docking, and mutagenesis. Heteronuclear NMR spectroscopy using variously N-15, C-13, H-2-enriched proteins enabled assignment of backbone resonances of more than 94% of the residues of both proteins. In a mixture of the two proteins at millimolar concentrations, complexation was inferred from perturbations of certain H-1-N-15 HSQC-resonances, which could be mapped to those residues involved at the interaction site. A docking computation using HADDOCK was employed constrained by the sites of interaction, to deduce an overall spatial structure of the complex. Contacts within the clytin-cgGFP complex and electrostatic complementarity of interaction surfaces argued for a weak protein-protein complex. A weak affinity was also observed by isothermal titration calorimetry (K-D = 0.9 mM). Mutation of clytin residues located at the interaction site reduced the degree of protein-protein association concomitant with a loss of effectiveness of cgGFP in color-shifting the bioluminescence. It is suggested that this clytin-cgGFP structure corresponds to the transient complex previously postulated to account for the energy transfer effect of GFP in the bioluminescence of aequorin or Renilla luciferase.

Держатели документа:
[Wang, Jinfeng] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
[Titushin, Maxim S.
Stepanyuk, Galina A.
Markova, Svetlana V.
Vysotski, Eugene S.] Russian Acad Sci, Inst Biophys, Siberian Branch, Lab Photobiol, Krasnoyarsk 660036, Russia
[Golz, Stefan] Bayer Schering Pharma AG, BSP GDD GTR TD GT, D-42096 Wuppertal, Germany
[Stepanyuk, Galina A.
Wang, Bi-Cheng
Lee, John] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Titushin, M.S.; Feng, Y.G.; Stepanyuk, G.A.; Li, Y...; Markova, S.V.; Golz, S...; Wang, B.C.; Lee, J...; Wang, J.F.; Vysotski, E.S.; Liu, Z.J.

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Cell-free bioluminescent screening of translation inhibitors [Text] / A. Y. Gorokhovatsky [et al.] // Biotechnol. Appl. Biochem. - 1998. - Vol. 27. - P259-263. - Cited References: 21 . - ISSN 0885-4513

РУБ Biochemistry & Molecular Biology + Biotechnology & Applied Microbiology
Рубрики:
DIPHTHERIA-TOXIN
   MESSENGER-RNA
   SYSTEM
   PROTEINS
Аннотация: The possibility of creating new screening methods with a cell-free translation system has been demonstrated with a quantitative determination of diphtheria toxin and some antibiotics (puromycin, kanamycin and tetracycline) as examples. The approach proposed follows from the ability of various substances to inhibit protein synthesis. We used a wheat-germ cell-free translation system stabilized by freeze-drying in the presence of trehalose with the mRNA of the Ca2+-activated photoprotein obelin as a reporter template. This freeze-dried cell-free translation system allows prolonged storage of the detecting system before it is required, increases the reproducibility of the results and simplifies the application procedure. The obelin mRNA extends the sensitivity of the method owing to the high sensitivity of detection of the synthesized protein.

Держатели документа:
Russian Acad Sci, Branch Inst Bioorgan Chem, Pushchino 142292, Moscow Region, Russia
RAS, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Tech Univ Berlin, Inst Biochem & Mol Biol, D-10587 Berlin, Germany
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gorokhovatsky, A.Y.; Shaloiko, L.A.; Bondar, V.S.; Vysotski, E.S.; Maximov, E.E.; von Doehren, H...; Alakhov, Y.B.

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    Application of enzyme bioluminescence in ecology [Text] / V. . Kratasyuk, E. . Esimbekova // Luminescence. - 2014. - Vol. 29. - P25-25. - Cited References: 6 . - ISSN 1522-7235. - ISSN 1522-7243
Рубрики:
SYSTEM
   WATER
   ASSAY

WOS
Держатели документа:
[Kratasyuk, Valentina
Esimbekova, Elena] Siberian Fed Univ, Krasnoyarsk, Russia
[Kratasyuk, Valentina
Esimbekova, Elena] RAS, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V...; Esimbekova, E...

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Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation [Text] / T. V. Kobzeva [et al.] // Luminescence. - 2014. - Vol. 29, Is. 7. - P703-710, DOI 10.1002/bio.2656. - Cited References: 29. - The work was supported by the Program of Siberian Branch of Russian Academy of Sciences (project no. 71), Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh 2272.2012.3), the Russian Foundation for Basic Research (project no. 12-03-33082), and the Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" (grant no. 11.G34.31.0058). . - ISSN 1522-7235. - ISSN 1522-7243

РУБ Biochemistry & Molecular Biology
Рубрики:
BIOLUMINESCENCE
   COMPONENTS
   MECHANISMS
   SYSTEM
Кл.слова (ненормированные):
Higher luminous fungi -- Neonothopanus nambi -- ionizing irradiation -- reactive oxygen species -- lipid peroxidation
Аннотация: The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed withmyceliumon a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production. Copyright (C) 2014 John Wiley & Sons, Ltd.

WOS
Держатели документа:
[Kobzeva, Tatiana V.
Melnikov, Anatoly R.
Karogodina, Tatiana Y.
Zikirin, Samat B.
Stass, Dmitri V.
Molin, Yuri N.] Inst Chem Kinet & Combust SB RAS, Novosibirsk 630090, Russia
[Melnikov, Anatoly R.
Zikirin, Samat B.
Stass, Dmitri V.] Novosibirsk State Univ, Novosibirsk 630090, Russia
[Rodicheva, Emma K.
Medvedeva, Svetlana E.
Puzyr, Alexey P.
Bondar, Vladimir S.
Gitelson, Joseph I.] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
[Rodicheva, Emma K.
Medvedeva, Svetlana E.
Puzyr, Alexey P.
Burov, Andrey A.
Bondar, Vladimir S.
Gitelson, Joseph I.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Burov, Andrey A.] Special Design Technol Bur Nauka SB RAS, Krasnoyarsk 660049, Russia
ИБФ СО РАН
СКТБ : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kobzeva, T.V.; Melnikov, A.R.; Karogodina, T.Y.; Zikirin, S.B.; Stass, D.V.; Molin, Y.N.; Rodicheva, E.K.; Medvedeva, S.E.; Puzyr, A.P.; Burov, A.A.; Bondar, V.S.; Gitelson, J.I.; Program of Siberian Branch of Russian Academy of Sciences [71]; Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools [NSh 2272.2012.3]; Russian Foundation for Basic Research [12-03-33082]; Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" [11.G34.31.0058]

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Increase of atmospheric CO2: Response patterns of a simple terrestrial man-made ecosystem [Text] / L. A. Somova, N. S. Pechurkin, T. I. Pisman ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1731-1735, DOI 10.1016/S0273-1177(03)00110-8. - Cited References: 22 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
ELEVATED CARBON-DIOXIDE
   MATHEMATICAL-MODEL
   MICROORGANISMS
   NITROGEN
   LIMITATION
   ENRICHMENT
   DYNAMICS
   SYSTEM
Аннотация: Simple models of terrestrial ecosystems with a limited number of components are an efficient tool to study the main laws of functioning of populations, including microbial ones, and their communities, as components of natural ecosystems, under variable environmental conditions. Among other factors are the increase of carbon dioxide in the atmosphere and limitation of plants' growth by biogenic elements. The main types of ecosystems' responses to changes in environmental conditions (a change in CO2 concentration) have been demonstrated in a "plants - rhizospheric microorganisms - artificial soil" simple experimental system. The mathematical model of interactions between plants and microorganisms under normal and elevated atmospheric CO2 and limitation by nutrients (nitrogen and phosphorus) yielded a qualitative agreement between calculated and experimental values of limiting substances concentrations and release rates of exudates. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Pisman, T.I.; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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Light intensity and production parameters of phytocenoses cultivated on soil-like substrate under controled environment conditions [Text] / A. A. Tikhomirov [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1775-1780, DOI 10.1016/S0273-1177(03)00120-0. - Cited References: 11 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
SYSTEM
Аннотация: To increase the degree of closure of biological life support systems of a new generation, we used vermicomposting to involve inedible phytomass in the intra-system mass exchange. The resulting product was a soil-like substrate, which was quite suitable for growing plants (Manukovsky et al. 1996, 1997). However, the soil-like substrate can be regarded as a candidate for inclusion in a system only after a comprehensive examination of its physical, chemical, and other characteristics. An important criterion is the ability of the soil-like substrate to supply the necessary mineral elements to the photosynthesizing component under the chosen cultivation conditions. Thus, the purpose of this work was to study the feasibility of enhancing the production activity of wheat and radish crops by varying the intensity of photosynthetically active radiation, without decreasing the harvest index. The increase of light intensity from 920 to 1150 mumol(.)m(-2.)s(-1) decreased the intensity of apparent photosynthesis of the wheat crops and slightly increased the apparent photosynthesis of the radish crops The maximum total and grain productivity (kg/m(2)) of the wheat crops was attained at the irradiance of 920 mumol(.)m(-2.)s(-1). Light intensity of 1150 mumol(.)m(2.)s(-1) decreased the productivity of wheat plants and had no significant effect on the productivity of the radish crops (kg/m(2)) as compared to 920 mumol(.)m(-2.)s(-2). The qualitative and quantitative composition of microflora of the watering solution and substrate was determined by the condition of plants, developmental phase and light intensity. By the end of wheat growth under 1150 mumol(.)m(-2.)s(-1) the numbers of bacteria of the coliform family and phytopathogenic bacteria in the watering solution and substrate were an order of magnitude larger than under other illumination conditions. The obtained data suggest that the cultivation of plants in a life support system on soil-like substrate from composts has a number of advantages over the cultivation on neutral substrates, which require continual replenishment of the plant nutrient solution from the system's store to complement the macro- and micro- elements. Yet, a number of problems arise, including those related to the controlling of the production activity of the plants by the intensity of photosynthetically active radiation. It is essential to understand why the intensity of production processes is limited at higher irradiation levels and to overcome the factors responsible for this, so that the soil-like substrate could have an even better chance in the competition for the best plant cultivation technology to be used in biological life support systems. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
RAS SB, Inst Biophys, Krasnoyarsk 660036, Russia
Tomsk VV Kuibyshev State Univ, Tomsk 634050, Russia
Univ Clermont Ferrand, LGCB, F-63174 Aubiere, France
European Space Agcy, European Space Technol Ctr, NL-2200 AG Noordwijk, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Gribovskaya, I.A.; Tirranen, L.S.; Manukovsky, N.S.; Zolotukhin, I.G.; Karnachuk, R.A.; Gros, J.B.; Lasseur, C...; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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Characteristics of enclogenous flavin fluorescence of Photobacterium leiognathi luciferase and Vibrio fischeri NAD(P)H : FMN-oxidoreductase [Text] / E. V. Vetrova [et al.] // Luminescence. - 2005. - Vol. 20: 11th International Symposium on Luminescence Spectrometry - Detection Techniques in Biomedical and Environmental Analysis (MAY 05-08, 2004, Beijing, JAPAN), Is. 3. - P. 205-209, DOI 10.1002/bio.815. - Cited References: 22 . - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology
Рубрики:
FLAVODOXIN
   ANISOTROPY
   REDUCTASE
   DYNAMICS
   SYSTEM
Кл.слова (ненормированные):
bacterial bioluminescence -- flavin fluorescence
Аннотация: The bioluminescent bacterial enzyme system NAD(P)H:FMN-oxidoreductase-luciferase has been used as a test system for ecological monitoring. One of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. The use of endogenous flavin fluorescence for investigation of the interactions of non-fluorescent compounds with the bacterial luciferase from Photobacterium leiognathi and NAD(P)H:FMN-oxidoreductase from Vibrio fischeri has been proposed. Fluorescence spectroscopy methods have been used to study characteristics of endogenous flavin fluorescence (fluorophore lifetime, the rotational correlation time). The fluorescence anisotropy behaviour of FMN has been analysed and compared to that of the enzyme-bound flavin. The fluorescence characteristics of endogenous flavin of luciferase and NAD(P)H:FMN-oxidoreductase have been shown to be applicable in studying enzymes' interactions with non-fluorescent compounds. Copyright (c) 2005 John Wiley & Sons, Ltd.

WOS
Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
Univ Wageningen & Res Ctr, MicroSpect Ctr, NL-6703 HA Wageningen, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vetrova, E.V.; Kudryasheva, N.S.; Visser, AJWG; van Hoek, A...

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A hypothesis about double surging climate change in the 20th century [Text] / P. V. Belolipetskii, S. I. Bartsev, A. G. Degermendzhi // Dokl. Earth Sci. - 2015. - Vol. 460, Is. 1. - P46-49, DOI 10.1134/S1028334X1501002X. - Cited References:15. - This work was supported by the Program of presidium of Russian Academyof Science "Research Scientific Studies for Development of Arctic Zoneof the Russian Federation," project no. 12. . - ISSN 1028-334X. - ISSN 1531-8354

РУБ Geosciences, Multidisciplinary
Рубрики:
REGIME SHIFTS
   NORTH PACIFIC
   OCEAN
   SYSTEM
   HEAT

WOS,
Scopus
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Computat Modeling, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.
ИВМ СО РАН
ИБФ СО РАН

Доп.точки доступа:
Belolipetskii, P.V.; Bartsev, S.I.; Degermendzhi, A.G.; presidium of Russian Academy of Science "Research Scientific Studies forDevelopment of Arctic Zone of the Russian Federation" [12]

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10.

Total peroxidase and catalase activity of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in comparison with the level of light emission [Text] / O. A. Mogil'naya [et al.] // Appl. Biochem. Microbiol. - 2015. - Vol. 51, Is. 4. - P419-424, DOI 10.1134/S0003683815040110. - Cited References:35. - The authors are grateful to N. V. Psurtseva (curator of the collection of basidiomycetes of the Botanical Institute, Russian Academy of Science) for help with the species affiliation of the IBSO 2328 culture. This work was supported by the Program of Interdisciplinary Projects of the Siberian Branch of the Russian Academy of Sciences, project no. 71. . - ISSN 0003-6838. - ISSN 1573-8183

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
OXIDATIVE STRESS
   SYSTEM
   FUNGI
   BIOLUMINESCENCE
   LUMINESCENCE
Кл.слова (ненормированные):
basidiomycetes -- luminescence -- peroxidase -- catalase
Аннотация: The peroxidase and catalase activities in the mycelium of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in normal conditions and under stress were compared. An increase in the luminescence level was observed under stress, as well as an increase in peroxidase and catalase activities. Moreover, the peroxidase activity in extracts of A. borealis mycelium was found to be almost one and a half orders of magnitude lower, and the catalase activity more than two orders of magnitude higher in comparison with the N. nambi mycelium. It can be suggested that the difference between the brightly luminescent and dimly luminescent mycelium of N. nambi is due to the content of (HO2)-O-2 or other peroxide compounds.

WOS,
Scopus
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Mogil'naya, O. A.; Ronzhin, N. O.; Medvedeva, S. E.; Bondar', V. S.; Siberian Branch of the Russian Academy of Sciences [71]

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11.

SIMILARITIES AND DIFFERENCES BETWEEN THE CHAETOPTERUS VARIOPEDATUS POLYCHAETE LUCIFERASES DEPENDING ON THE TYPE OF HABITAT / K. V. Purtov, V. N. Petushkov, N. S. Rodionova [et al.] // Bull. Russ. State Med. Univ. - 2021. - Is. 5. - P41-46, DOI 10.24075/vrgmu.2021.049. - Cited References:20 . - ISSN 2500-1094. - ISSN 2542-1204

РУБ Medicine, General & Internal
Рубрики:
BIOLUMINESCENCE
ANNELIDA
SYSTEM
Кл.слова (ненормированные):
bioluminescence -- luciferase -- polychaetes -- Chaetopterus variopedatus -- marine worms
Аннотация: The marine polychaete Chaetopterus variopedatus (Renier) (family Chaetopteridae) is a cosmopolitan species complex, consisting of distinct populations/subspecies. The worms release glowing (460 nm) clouds of mucus when disturbed, and their parapodia often glow brightly. Currently, it is still unclear how exactly the bioluminescence system of these polychaetes functions. It has been previously assumed that the C. variopedatus luciferase may be used for detection of ferroptosis, the recently explored pathway of programmed cell death, resulting from accumulation of the ferrous ions. This study was aimed to extract and characterize the C. variopedatus luciferases, as well as to compare luciferases obtained from C. variopedatus of different populations. When extracting the enzyme responsible for bioluminescence from the frozen samples of Brazilian C. variopedatus using the improved method, two active luciferases, L1 and L2, were obtained. We assumed that one of the listed above luciferases was responsible for luminescence of the mucus and the other luciferase was responsible for luminescence in parapodia, and used the method for the distinct samples of mucus and parapodia of the living Far Eastern C. variopedatus. However, mucus of the latter turned out to be non-glowing. It is shown that luciferase L2 is responsible for luminescence in the parapodia of the C. variopedatus polychaete, since this luciferase has been found in the total biomass of Brazilian polychaetes and parapodia of Far Eastern polychaetes. Luminescence of the Brazilian C. variopedatus mucus is attributed to the functioning of luciferase L1, which is lacking in the mucus of the Far Eastern subspecies. The range of luciferase isoforms in polychaetes C. variopedatus depends on the place of origin.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk Sci Ctr, Siberian Branch, Moscow, Russia.
Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia.
Pacific State Med Univ, Vladivostok, Russia.
Pirogov Russian Natl Res Med Univ, Moscow, Russia.

Доп.точки доступа:
Purtov, K., V; Petushkov, V. N.; Rodionova, N. S.; Chepurnykh, T., V; Kozhemyako, V. B.; Zagitova, R., I; Shcheglov, A. S.; Ziganshin, R. H.; Tsarkova, A. S.

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