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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zamay G. S., Zamay T. N., Kolovskii V. A., Shabanov A. V., Glazyrin Y. E., Veprintsev D. V., Krat A. V., Zamay S. S., Kolovskaya O. S., Gargaun A., Sokolov A. E., Modestov A. A., Artyukhov I. P., Chesnokov N. V., Petrova M. M., Berezovski M. V., Zamay A. S.
Заглавие : Electrochemical aptasensor for lung cancer-related protein detection in crude blood plasma samples
Коллективы : Ministry of Education and Science of Russian Federation Federal Target Program [14.604.21.0105]
Место публикации : Sci. Rep. - 2016. - Vol. 6. - Ст.34350. - ISSN 2045-2322, DOI 10.1038/srep34350
Примечания : Cited References:24. - Authors thank Dr. Mahmoud Labib and Galina Kudryasheva. This work was supported by Ministry of Education and Science of Russian Federation Federal Target Program #14.604.21.0105 for Anna S. Zamay. Electron microscopy was carried out at the Multiple-Access Center of Krasnoyarsk Research Center Siberian branch of Russian Academy of Science.
Предметные рубрики: SQUARE-WAVE VOLTAMMETRY
GENE-EXPRESSION
SERUM
BIOSENSORS
DIAGNOSIS
ADENOCARCINOMA
BIOMARKERS
SENSOR
Аннотация: The development of an aptamer-based electrochemical sensor for lung cancer detection is presented in this work. A highly specific DNA-aptamer, LC-18, selected to postoperative lung cancer tissues was immobilized onto a gold microelectrode and electrochemical measurements were performed in a solution containing the redox marker ferrocyanide/ferricyanide. The aptamer protein targets were harvested from blood plasma of lung cancer patients by using streptavidin paramagnetic beads and square wave voltammetry of the samples was performed at various concentrations. In order to enhance the sensitivity of the aptasensor, silica-coated iron oxide magnetic beads grafted with hydrophobic C8 and C4 alkyl groups were used in a sandwich detection approach. Addition of hydrophobic beads increased the detection limit by 100 times. The detection limit of the LC-18 aptasensor was enhanced by the beads to 0.023 ng/mL. The formation of the aptamer -protein -bead sandwich on the electrode surface was visualized by electron microcopy. As a result, the electrochemical aptasensor was able to detect cancer-related targets in crude blood plasma of lung cancer patients.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Komina A. V., Yaroslavtsev R. N., Gerasimova Yu. V., Stolyar S. V., Olkhovsky I. A., Bairmani M. S.
Заглавие : Magnetic Nanoparticles for Extracting DNA from Blood Cells
Место публикации : Bull. Russ. Acad. Sci. Phys. - 2020. - Vol. 84, Is. 11. - P.1362-1365. - ISSN 10628738 (ISSN), DOI 10.3103/S1062873820110155
Примечания : Cited References: 15
Аннотация: A technique for extracting DNA from blood cells using magnetic particles offers the advantage of saving time and prospects of automating the extraction process. A way of obtaining magnetic iron nanoparticles for extracting DNA from blood cells is developed. Magnetic nanoparticles with characteristics suitable for extracting genomic DNA from leukocytes are obtained and investigated.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tyumentseva A. V., Gorbenko A. S., Yaroslavtsev R. N., Stolyar S. V., Gerasimova Yu. V., Komogortsev S. V., Bayukov O. A., Knyazev Yu. V., Volochaev M. N., Olkhovskiy I. A., Iskhakov R. S.
Заглавие : Iron oxide nanoparticles for isolating DNA from blood cells
Место публикации : Bull. Russ. Acad. Sci. Phys. - 2021. - Vol. 85, Is. 9. - P.965-969. - ISSN 10628738 (ISSN), DOI 10.3103/S1062873821090185
Примечания : Cited References: 13. - This work was supported by the Russian Foundation for Basic Research; the Government of Krasnoyarsk Territory; the Krasnoyarsk Regional Fund for the Support of Scientific and Scientific and Technical Activities, project no. 20-42-242902; and the RF Presidential Council of Grants for the State Support of Young Russian Scientists (Candidates of Science), project no. MK-1263.2020.3
Аннотация: Magnetic iron oxide nanoparticles for separating DNA from blood cells are synthesized. Magnetic nanoparticles with a silicate coating are obtained, and their physical and chemical properties are studied. The possibility of using the nanocomposites to isolate DNA from leukocytes for hematological studies is confirmed experimentally.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolovskaya, Olga S., Zyuzyukina, Alena V., Dassie, Justin P., Zamay, Galina S., Zamay, Tatiana N., Boyakova, Nina V., Khorzhevskii, Vladimir A., Kirichenko, Daria A., Lapin I. N., Shchugoreva, Irina A., Artyushenko, Polina V., Tomilin F. N., Veprintsev, Dmitry V., Glazyrin, Yury E., Minic, Zoran, Bozhenko, Vladimir K., Kudinova, Elena A., Kiseleva, Yana Y., Krat, Alexey V., Slepov, Eugene V., Bukatin, Anton S., Zukov, Ruslan A., Shesternya, Pavel A., Berezovski, Maxim V., Giangrande, Paloma H., Kichkailo, Anna S.
Заглавие : Monitoring of breast cancer progression via aptamer-based detection of circulating tumor cells in clinical blood samples
Место публикации : Front. Mol. Biosci. - 2023. - Vol. 10. - Ст.1184285. - ISSN 2296889X (eISSN), DOI 10.3389/fmolb.2023.1184285
Примечания : Cited References: 37. - CTCs isolation method development and clinical sample analyses were supported by the Ministry of Healthcare of Russian Federation project REYC-2023-0012. The Ministry of Science and Higher Education of the Russian Federation project FWES-2022-0005 supported aptamer characterization, molecular modeling. MDA-231/LUC cells were from R. Domann (Jenkin et al. Breast Cancer Research, 2005). Technical and instrumental support was provided by the Shared Core Facilities of Molecular and Cell Technologies at Krasnoyarsk State Medical University and Krasnoyarsk Regional Centre for Collective Use at the Federal Research Centre “KSC SB RAS.” The confocal fluorescence microscopy research was carried out with the equipment of the Tomsk Regional Core Shared Research Facilities Center of the National Research Tomsk State University. The Center was supported by the Ministry of Science and Higher Education of the Russian Federation, grant no. 075-15-2021-693 (No. 13.RFC.21.0012)The authors are grateful to all the patients and hospital staff participating in this research. We acknowledge the assistance of the AptamerLab LCC (www.aptamerlab.com) and personally Vasily Mezko for the technical support. The authors thank Valentina L. Grigoreva, and Irina V. Gildebrand for the help with СTC staining
Аннотация: Introduction: Breast cancer (BC) diagnostics lack noninvasive methods and procedures for screening and monitoring disease dynamics. Admitted CellSearch® is used for fluid biopsy and capture of circulating tumor cells of only epithelial origin. Here we describe an RNA aptamer (MDA231) for detecting BC cells in clinical samples, including blood. The MDA231 aptamer was originally selected against triple-negative breast cancer cell line MDA-MB-231 using cell-SELEX. Methods: The aptamer structure in solution was predicted using mFold program and molecular dynamic simulations. The affinity and specificity of the evolved aptamers were evaluated by flow cytometry and laser scanning microscopy on clinical tissues from breast cancer patients. CTCs were isolated form the patients’ blood using the developed method of aptamer-based magnetic separation. Breast cancer origin of CTCs was confirmed by cytological, RT-qPCR and Immunocytochemical analyses. Results: MDA231 can specifically recognize breast cancer cells in surgically resected tissues from patients with different molecular subtypes: triple-negative, Luminal A, and Luminal B, but not in benign tumors, lung cancer, glial tumor and healthy epithelial from lungs and breast. This RNA aptamer can identify cancer cells in complex cellular environments, including tumor biopsies (e.g., tumor tissues vs. margins) and clinical blood samples (e.g., circulating tumor cells). Breast cancer origin of the aptamer-based magnetically separated CTCs has been proved by immunocytochemistry and mammaglobin mRNA expression. Discussion: We suggest a simple, minimally-invasive breast cancer diagnostic method based on non-epithelial MDA231 aptamer-specific magnetic isolation of circulating tumor cells. Isolated cells are intact and can be utilized for molecular diagnostics purposes.
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