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Вид документа : Статья из журнала
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Автор(ы) : Tyumentseva A. V., Gorbenko A. S., Yaroslavtsev R. N., Stolyar S. V., Gerasimova Yu. V., Komogortsev S. V., Bayukov O. A., Knyazev Yu. V., Volochaev M. N., Olkhovskiy I. A., Iskhakov R. S.
Заглавие : Iron oxide nanoparticles for isolating DNA from blood cells
Место публикации : Bull. Russ. Acad. Sci. Phys. - 2021. - Vol. 85, Is. 9. - P.965-969. - ISSN 10628738 (ISSN), DOI 10.3103/S1062873821090185
Примечания : Cited References: 13. - This work was supported by the Russian Foundation for Basic Research; the Government of Krasnoyarsk Territory; the Krasnoyarsk Regional Fund for the Support of Scientific and Scientific and Technical Activities, project no. 20-42-242902; and the RF Presidential Council of Grants for the State Support of Young Russian Scientists (Candidates of Science), project no. MK-1263.2020.3
Аннотация: Magnetic iron oxide nanoparticles for separating DNA from blood cells are synthesized. Magnetic nanoparticles with a silicate coating are obtained, and their physical and chemical properties are studied. The possibility of using the nanocomposites to isolate DNA from leukocytes for hematological studies is confirmed experimentally.
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Вид документа : Статья из журнала
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Автор(ы) : Morozov E. V., Falaleev O. V., Martyanov O. N.
Заглавие : New insight into the wax precipitation process: in situ NMR imaging study in a cold finger cell
Место публикации : Energy Fuels: American Chemical Society, 2016. - Vol. 30, Is. 11. - P.9003-9013. - ISSN 08870624 (ISSN), DOI 10.1021/acs.energyfuels.6b01535
Примечания : Cited References: 69. - This research was performed with the financial support of the Russian Science Foundation (Project No. 15-19-00119).
Ключевые слова (''Своб.индексиров.''): cells--crude oil--cytology--deposits--diffusion--gelation--magnetic resonance imaging--ostwald ripening--phase separation--polymer blends--deposit formation--deposit structures--diffusion controlled growth--dominant mechanism--lower temperatures--morphology and dynamics--phase-separation process--temperature regimes--image processing
Аннотация: A cold finger cell intended for the wax deposition measurements was fabricated and integrated into an NMR imaging probe for the noninvasive study of wax precipitation processes in situ. The cell was first tested with a model system; then, a series of experiments with different thermal gradients applied to the cell were performed for a waxy crude oil. NMR imaging of the operating cell revealed the formation of a deposit with the morphology and dynamics strongly correlating with the temperature regime. At higher temperatures of cold finger, the incipient wax gel ages uniformly, giving rise to the hard and thin inner layer of deposit accompanied by a branched loosely consolidated outer layer. Conversely, the lower temperatures facilitate formation of a thick deposit which no longer ages uniformly and slow down the diffusion-controlled growth of the branched deposit structure. The results obtained are consistent with the majority of the data previously reported. Thus, gelation of the wax at temperatures below the cloud point and subsequent thermal-driven diffusion processes are considered to be the dominant mechanisms of the deposit formation. The counter diffusion and Ostwald ripening aging concepts were found to be relevant in the case of the cold finger study and account for the phenomena observed in this work. The information obtained via NMR imaging is highly complementary to the results obtained by other techniques that can aid in understanding the essential processes behind the wax precipitation phenomena. The approach developed can be effectively extended to study any thermal-driven phase separation process. © 2016 American Chemical Society.
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