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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Menzyanova N. G., Pyatina S. А., Nikolaeva E. D., Shabanov A. V., Nemtsev I. V., Stolyarov D. P., Dryganov D. B., Sakhnov E. V., Shishatskaya E. I.
Заглавие : Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients
Место публикации : Toxicol. Rep. - 2019. - Vol. 6. - P.74-90. - ISSN 22147500 (ISSN), DOI 10.1016/j.toxrep.2018.11.009
Примечания : Cited References: 85. - The study was supported by the Russian Science Foundation , Project No 17-15-01352 .
Ключевые слова (''Своб.индексиров.''): monocytes--macrophages--cell morphology--polyhydroxyalkanoates--atherosclerosis--intravascular stenting
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Menzyanova, Natalia G., Pyatina, Svetlana V., Nikolaeva, Elena D., Shabanov A. V., Nemtsev I. V., Stolyarov D. P., Dryganov, Dmitry B., Sakhnov, Eugene V., Vinokurova, Daria A., Shishatskaya, Ekaterina I.
Заглавие : Morphological aspects of monocyte/macrophage polarization on biopolymer scaffolds in atherosclerosis patients
Место публикации : J. Biotechnol. Biomater. - 2018. - Vol. 8, Is. 3. - Ст.1000284. - ISSN 2155-952X, DOI 10.4172/2155-952X.1000284
Примечания : Cited References: 67. - The study was supported by the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505).
Ключевые слова (''Своб.индексиров.''): monocytes--macrophages--cell morphology--polyhydroxyalkanoates--pha--copolymers--atherosclerosis--intravascular stenting
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured substrates, made from polyhydroxyalcanoates (PHAs) of various monomer compositions, prepared by casting polymer solutions on a defatted glass and followed by the solvent evaporation. Its surface relief, being further in direct contact with human cells in vitro was analyzed by AFM and SEM scanning. It was shown, that the features of micro/nano relief depend on the monomeric composition of polymer substrates. Monocytes (MNs) of patients with atherosclerosis and cardiac ischemia undergoing stenting and conventional anti-atherosclerotic therapy were harvested prior to and after stenting. After isolation MNs were cultured and transformed into MPh in direct contact with biopolymer culture substrates with different monomer composition and Nano-reliefs in comparison with culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described and their ratios for five samples of polymers were counted as an intermediate result in the development of “smart” material for blood vessel recovery. The results obtained allow us to assume that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates but also on the initial state of MNs in vivo and the general response of patients.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Menzyanova N. G., Pyatina S. A., Shabanov A. V., Nemtsev I. V., Stolyarov D. P., Dryganov D. B., Sakhnov E. V., Shishatskaya E. I.
Заглавие : The morphology and phenotype of monocyte-macrophages when cultured on bionanofilms substrates with different surface relief profiles
Место публикации : Biomolecules. - 2020. - Vol. 10, Is. 1. - Ст.65. - ISSN 2218273X (ISSN), DOI 10.3390/biom10010065
Примечания : Cited References: 40. - This research was funded by the Russian Science Foundation, Project No 17-15-01352
Аннотация: The effect of surface relief profiles of alkanoate-based bionanofilms to the monocyte-macrophages (MN-MPhs) from peripheral blood of patients with atherosclerosis was studied in vitro. Patients were subjected to coronary stenting. Cell morphology and phenotype (expression of CD antigens, levels of production of marker cytokines) in vitro were analyzed before and after the installation of stents. It was shown, that the mean square roughness (Rq) of the bionanofilms determined the variability of cell morphology, CD antigens spectraand activity of production interleukins-6 and-10. Also, it was revealed, that the “activity” of the surface topography of biopolymer substrates depends on the functional state of MNs, isolated in different time points: Before and after stenting the ratios of cell morphotypes and production of cytokines in MN-MPhs differed significantly.
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