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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Мензянова Н. Г., Николаева Е. Д., Винокурова Д. В., Шабанов, Александр Васильевич, Шершнева А. М., Шишацкая, Екатерина Игоревна, Сухова К. В.
Заглавие : Влияние биоматериалов медицинского назначения на структурно-функциональные особенности моноцитов
Место публикации : Журн. СФУ. Сер. "Биология". - 2016. - Т. 9, № 1. - С. 33-42. - ISSN 1997-1389, DOI 10.17516/1997-1389-2015-9-1-33-42
Ключевые слова (''Своб.индексиров.''): biomaterials for medical uses--polyhydroxyalkanoates--monocytes--nanodiamonds--fullerenes--биоматериалы медицинского назначения--моноциты--наноалмазы--фуллерены--полигидроксиалканоаты
Аннотация: Изучены морфофункциональные особенности моноцитов в условиях кратковременного культивирования in vitro на носителях из биополимера полигидроксиалканоата (ПГА) в присутствии наноалмазов (НА) и фуллеренов (Ф). Показано, что НА и Ф в исследованных концентрациях не влияли на жизнеспособность моноцитов в кратковременной культуре. Соотношение морфологических классов моноцитов в присутствии НА и Ф существенно отличалось от контрольного варианта культивирования. Влияние НА и Ф характеризовалось дозовой зависимостью. Воздействие наночастиц на культуру моноцитов значительно изменялось при формировании белковой короны на поверхности НА и Ф, что приводило к возникновению новых количественных соотношений между морфологическими классами моноцитов в культуре in vitro. Полученные результаты позволяют рассматривать кратковременную культуру моноцитов in vitro как адекватную модельную систему для оценки влияния новых материалов на процессы дифференцировки субпопуляций моноцитов.The morphological and functional features of the monocytes in a short-term in vitro cultivation on substrates of biopolymer polyhydroxyalkanoate (PHA) in the presence of nanodiamonds (ND) and fullerene (F) were studied. It has been shown that ND and F in the studied concentrations did not affect the viability of monocytes in the short-term culture. The ratio of morphological classes in the presence of ND and F signi?cantly different from the control group of cultivation. Effects of NA and F were characterized by dose-dependent. The formation of a protein corona on the surface of NA and F led to signi?cant changes in the effects of nanoparticles and the formation of new quantitative relationships between morphological classes of monocytes in culture in vitro. The results allow to consider a short-term culture of monocytes in vitro as a model system for adequate assessment of the impact of new materials on the processes of differentiation of monocyte subpopulations.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E. I., Nikitovic D., Shabanov A. V., Tzanakakis G. N., Tsatsakis A. M., Menzianova N. G.
Заглавие : Short-term culture of monocytes as an in vitro evaluation system for bionanomaterials designated for medical use
Место публикации : Food Chem. Toxicol.: Elsevier, 2016. - Vol. 96. - P.302-308. - ISSN 02786915 (ISSN), DOI 10.1016/j.fct.2016.08.025
Примечания : Cited References: 46
Предметные рубрики: MAGNETIC NANOPARTICLES
CELLULAR REDUCTION
PROTEIN CORONA
CELLS
MECHANOTRANSDUCTION
NANOMATERIALS
LOCALIZATION
BIOMATERIALS
CYTOSKELETON
ACTIVATION
Ключевые слова (''Своб.индексиров.''): biocompatible biopolymers--polyhydroxyalkanoates--nanomaterials--nanodiamonds--fullerenes--bio-nanomaterials--monocytes
Аннотация: We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the viability/metabolic activity of cells. The scanning electron microscopy assay can detect fine changes in cell morphology. In the present study BNM have been shown to affect; in a size, chemical composition and morphological characteristics-dependent manner, the ability of monocytes to reduce MTT as well as their morphology. Moreover, the specific effects of ND and F on MTT reduction and cell morphology were exhibited in a dose-dependent manner and sensitive to the formation of surface protein corona. Our results suggest that short-term culture of monocytes is a sensitive model system for assessing the biological effects of BMPs in vitro. © 2016 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Menzyanova N. G., Pyatina S. А., Nikolaeva E. D., Shabanov A. V., Nemtsev I. V., Stolyarov D. P., Dryganov D. B., Sakhnov E. V., Shishatskaya E. I.
Заглавие : Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients
Место публикации : Toxicol. Rep. - 2019. - Vol. 6. - P.74-90. - ISSN 22147500 (ISSN), DOI 10.1016/j.toxrep.2018.11.009
Примечания : Cited References: 85. - The study was supported by the Russian Science Foundation , Project No 17-15-01352 .
Ключевые слова (''Своб.индексиров.''): monocytes--macrophages--cell morphology--polyhydroxyalkanoates--atherosclerosis--intravascular stenting
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Menzyanova, Natalia G., Pyatina, Svetlana V., Nikolaeva, Elena D., Shabanov A. V., Nemtsev I. V., Stolyarov D. P., Dryganov, Dmitry B., Sakhnov, Eugene V., Vinokurova, Daria A., Shishatskaya, Ekaterina I.
Заглавие : Morphological aspects of monocyte/macrophage polarization on biopolymer scaffolds in atherosclerosis patients
Место публикации : J. Biotechnol. Biomater. - 2018. - Vol. 8, Is. 3. - Ст.1000284. - ISSN 2155-952X, DOI 10.4172/2155-952X.1000284
Примечания : Cited References: 67. - The study was supported by the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505).
Ключевые слова (''Своб.индексиров.''): monocytes--macrophages--cell morphology--polyhydroxyalkanoates--pha--copolymers--atherosclerosis--intravascular stenting
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured substrates, made from polyhydroxyalcanoates (PHAs) of various monomer compositions, prepared by casting polymer solutions on a defatted glass and followed by the solvent evaporation. Its surface relief, being further in direct contact with human cells in vitro was analyzed by AFM and SEM scanning. It was shown, that the features of micro/nano relief depend on the monomeric composition of polymer substrates. Monocytes (MNs) of patients with atherosclerosis and cardiac ischemia undergoing stenting and conventional anti-atherosclerotic therapy were harvested prior to and after stenting. After isolation MNs were cultured and transformed into MPh in direct contact with biopolymer culture substrates with different monomer composition and Nano-reliefs in comparison with culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described and their ratios for five samples of polymers were counted as an intermediate result in the development of “smart” material for blood vessel recovery. The results obtained allow us to assume that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates but also on the initial state of MNs in vivo and the general response of patients.
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