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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Semina P. N., Isaev I. L., Komogortsev S. V., Klyuchantsev A. B., Kostyukov A. S., Blagodatova A. V., Khrennikov D. E., Kichkailo A. S., Zamay T. N., Lapin I. N., Sokolov A. Е., Polyutov S. P., Karpov S. V.
Заглавие : Towards understanding the triggering of the malignant cell death in high-efficiency magneto-mechanical anticancer therapy
Место публикации : J. Phys. D. - 2023. - Vol. 56, Is. 6. - Ст.065401. - ISSN 00223727 (ISSN), DOI 10.1088/1361-6463/acb0dd. - ISSN 13616463 (eISSN)
Примечания : Cited References: 146. - P N S, A S K, D E K, S P P, S V K acknowledge the support by the Ministry of Science and High Education of Russian Federation (Project No. FSRZ-2020-0008). Experimental analyses were funded by the Ministry of Science and Higher Education of the Russian Federation (Project FWES-2022-0005) – A S K, T N Z. The authors thank Dr S V Saikova for providing the TEM image in figure (b)
Аннотация: The paper discusses schemes for the implementation of magneto-mechanical anticancer therapy and the most probable scenarios of damaging mechanical eﬀects on the membranes of malignant cells by targeted magnetic nanoparticles selectively bound to membrane mechanoreceptors employing aptamers. The conditions for selective triggering of the malignant cell apoptosis in a low-frequency non-heating alternating magnetic ﬁeld, corresponding to the exceeding threshold value of the force acting on the membrane and its mechanoreceptors, are established using a nanoparticle dynamic simulation. The requirements for the functionality of magnetic nanoparticles and their suitability for biomedical applications are analysed. Attention is paid to the possibility of the formation of magnetite nanoparticle aggregates in an external magnetic ﬁeld and their localization near tumor cell membranes. It is shown that the scenario involving the process of aggregation of magnetite nanoparticles provides a suﬃcient magneto-mechanical impact to achieve a therapeutic eﬀect. A possible explanation for the experimentally established fact of successful application of magneto-mechanical therapy using magnetite nanoparticles is presented, in which complete suppression of the Ehrlich carcinoma in an alternating magnetic ﬁeld as a response to a magnetome-chanical stimulus was demonstrated. This result conﬁrmed the possibility of using the method for high eﬃciency treatment of malignant neoplasms. The paper is provided with an extensive review of key publications and the state of art in this area.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Moryachkov R. V., Morozov D., Mironov V., Shchugoreva I., Artyushenko P. V., Zamay G. S., Molodenskiy D. S., Zabluda V. N., Kichkailo A.S., Sokolov A. Е.
Заглавие : The role of small-angle X-ray scattering and molecular simulations in 3D structure elucidation of a DNA aptamer-cancer cells magnetic separation agent
Коллективы : International Baltic Conference on Magnetism: focus on nanobiomedicine and smart materials, Балтийский федеральный университет им. И. Канта
Место публикации : 4th International Baltic Conference on Magnetism (IBCM 2021): Book of abstracts. - 2021. - P.168
Примечания : Cited References: 2. - The reported study was funded by RFBR, project number 19-32-90266
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Morozov D., Mironov V., Moryachkov R. V., Shchugoreva I. A., Artyushenko P. V., Zamay G. S., Kolovskaya O. S., Zamay T. N., Krat A. V., Molodenskiy D. S., Zabluda V. N., Veprintsev D. V., Sokolov A. Е., Zukov R. A., Berezovski M. V., Tomilin F. N., Fedorov D. G., Alexeev Y., Kichkailo A. S.
Заглавие : The role of SAXS and molecular simulations in 3D structure elucidation of a DNA aptamer against lung cancer
Место публикации : Mol. Ther. Nucl. Acids. - 2021. - Vol. 25. - P.316-327. - ISSN 21622531 (ISSN), DOI 10.1016/j.omtn.2021.07.015
Примечания : Cited References: 84. - The research was performed using equipment of the Shared Core Facilities of Molecular and Cell Technologies at Krasnoyarsk State Medical University. The synchrotron SAXS data were collected at beamline P12 operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). A.S.K. is grateful to Aptamerlab LLC for the assistance in aptamer design and 3D structure analyses. We thank Ivan Lapin for his help with microscopic analyses. Microscopic analyses using Carl Zeiss LSM 800 were carried out at the Center for Bioassay, Nanotechnology and Nanomaterials Safety (“Biotest-Nano”) (Multiple-Access Center, Tomsk State University, Tomsk, Russia). D.M. also thanks the CSC-IT Center in Espoo, Finland, for providing computational resources. The study was supported by a grant from the Russian Science Foundation (project number 21-73-20240) for A.S.K. R.V.M aknowledges Russian Foundation for Basic Research (project number 19-32-90266) for funding. D.G.F. acknowledges financial support by JSPS KAKENHI, grant number 19H02682. D.S.M. acknowledges financial support by BMBF grant number 16QK10A (SAS-BSOFT). Y.A.’s work at Argonne National Laboratory was supported by the US Department of Energy, Office of Science, under contract DE-AC02-06CH11357. D.M. received funding as a part of BioExcel CoE (https://bioexcel.eu/), a project funded by the European Union contracts H2020-INFRAEDI-02-2018-823830 and H2020-EINFRA-2015-1-675728. V.M. thanks Russian Foundation for Basic Research (project number 19-03-00043) for funding
Аннотация: Aptamers are short, single-stranded DNA or RNA oligonucleotide molecules that function as synthetic analogs of antibodies and bind to a target molecule with high specificity. Aptamer affinity entirely depends on its tertiary structure and charge distribution. Therefore, length and structure optimization are essential for increasing aptamer specificity and affinity. Here, we present a general optimization procedure for finding the most populated atomistic structures of DNA aptamers. Based on the existed aptamer LC-18 for lung adenocarcinoma, a new truncated LC-18 (LC-18t) aptamer LC-18t was developed. A three-dimensional (3D) shape of LC-18t was reported based on small-angle X-ray scattering (SAXS) experiments and molecular modeling by fragment molecular orbital or molecular dynamic methods. Molecular simulations revealed an ensemble of possible aptamer conformations in solution that were in close agreement with measured SAXS data. The aptamer LC-18t had stronger binding to cancerous cells in lung tumor tissues and shared the binding site with the original larger aptamer. The suggested approach reveals 3D shapes of aptamers and helps in designing better affinity probes.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya V. V., Goncharova N. S., Biriukov V. V., Bashmakova E. E., Kabilov M. R., Baykov I. K., Sokolov A. Е., Frank L. A.
Заглавие : The Ca2+-regulated photoprotein obelin as a tool for SELEX monitoring and DNA aptamer affinity evaluation
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]
Место публикации : Photochem. Photobiol. - 2020. - Vol. 96, Is. 5. - P.1041-1046. - ISSN 0031-8655, DOI 10.1111/php.13274. - ISSN 1751-1097(eISSN)
Примечания : Cited References: 25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531.
Предметные рубрики: CARDIAC TROPONIN-I
BIOLUMINESCENCE
IMMUNOASSAY
APTASENSOR
DIAGNOSIS
Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kim P. D., Zamay S. S., Zamay T. N., Prokopenko V. S., Kolovskaya O. S., Zamay G. S., Princ, V. Ya., Seleznev V. A., Komonov A. I., Spivak E. A., Rudenko R. Y., Dubinina A. V., Komarov A. V., Denisenko V. V., Komarova M. A., Sokolov A. E., Narodov A. A., Zjivaev V. P., Zamay A. S.
Заглавие : The antitumor effect of magnetic nanodisks and DNA aptamer conjugates
Коллективы : Russian Science Foundation [14-15-00805]
Место публикации : Dokl. Biochem. Biophys.: MAIK Nauka-Interperiodica / Springer, 2016. - Vol. 466, Is. 1. - P.66-69. - ISSN 1607-6729, DOI 10.1134/S1607672916010154. - ISSN 1608-3091(eISSN)
Примечания : Cited References:7. - This work was supported by the Russian Science Foundation (project no. 14-15-00805).
Предметные рубрики: Nanoparticles
Cells
Аннотация: Here we describe a method of forming large arrays (up to 109 pieces) of free magnetic Ni-nanodisks 50 nm thick coated on both sides with layers of 5 nm thick Au. The antitumor effect of the magnetic nickel gold-coated nanodisks and DNA aptamer conjugates was evaluated in vivo and in vitro. Under the influence of rotating magnetic field, the studied nanodisks can cause the death of Ehrlich ascites carcinoma cells.
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Moryachkov R. V., Zabluda V. N., Berlina A. N., Peters G. S., Kichkailo A. S., Sokolov A. Е.
Заглавие : Small-angle scattering applications to the analysis of aptamer structure and conformational changes
Коллективы : Internetional Conference on Synchrotron and Free Electron Laser Radiation: Generation and Application
Место публикации : Internetional Conference on Synchrotron and Free Electron Laser Radiation: Generation and Application (2020 ; 13-16 July ; Novosibirsk). AIP Conference Proceedings: book of abstracts. - 2020. - Vol. 2299. - Ст.040002. - , DOI 10.1063/5.0030394; \b Synchrotron and free electron laser radiation: generation and application (SFR-2020). - Novosibirsk. - P.45-46
Примечания : Cited References: 40. - The reported study was funded by RFBR, project number 19-32-90266 (investigation of the structure changes). This work was financially supported by Russian Science Foundation (project # 19-44-02020, obtaining of the complexes of aptamer and lead ions). We acknowledge the European Synchrotron Radiation Facility for provision of synchrotron radiation facilities (experiment #MX-2039) and we would like to thank Bart Van Laer for assistance in using beamline BM29
Аннотация: Aptamers, structured single-chain oligonucleotides, are promising tools for detection of a wide variety of compounds, from high to low molecular weight, and affecting on them. The aptamers that are most affine for a detectable compound are selected from the libraries of random sequences by the SELEX method (Systematic evolution of ligands by exponential enrichment). The reason why aptamers deserve a special consideration lies in the specific features of their structure and the mechanism of binding to their target. Aptamers can be exploited for metal-ion sensing, biosensing, drug delivery and other functions. To apply the oligonucleotides in the medicine, ecology, food production, agriculture, etc., we need to know how the aptamers bind to their targets, how they change their conformation upon specific binding and how the environment influences on the affinity of aptamers. Small-Angle X-ray scattering showed that the interaction of aptamers with heavy metal and other divalent ions proceeds according to different mechanisms, and the aptamers used undergo different conformational changes.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Moryachkov R. V., Zabluda V. N., Sokolov A. Е., Shchugoreva I. A., Tomilin F. N., Peters G., Spiridonova V. A.
Заглавие : Small-Angle scattering applications to the analysis of aptamer structure and conformational changes
Коллективы : Aptamers in Russia, international conference
Место публикации : Aptamers in Russia, international conference (1 ; 2019 ; Aug. 27-30 ; Krasnoyarsk). Molecular Therapy - Nucleic Acids: book of abstracts of the 1st Int. conf. "Aptamers in Russia 2019". - 2019. - Vol. 17, Suppl. 1. - P.4
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Moryachkov R., Spiridonova V. A., Zabluda V. N., Peters G., Sokolov A. E.
Заглавие : Small angle X-ray scattering on RE31 aptamer
Коллективы : Euro-Asian Symposium "Trends in MAGnetism", "Trends in MAGnetism", Euro-Asian Symposium, Институт физики им. Л.В. Киренского Сибирского отделения РАН
Место публикации : VI Euro-Asian Symposium "Trends in MAGnetism" (EASTMAG-2016): abstracts/ ed.: O. A. Maksimova, R. D. Ivantsov. - Krasnoyarsk: KIP RAS SB, 2016. - Ст.P12.3. - P.559-560. - ISBN 978-5-904603-06-9 (Шифр -478014040)
Примечания : References: 3. - The work was supported by Ministry of education and science of Russia (Agreement No.14.607.21.0104 (RFMEFI60714X0104))
Ключевые слова (''Своб.индексиров.''): saxs--aptamer--biomolecules structure--atsas--dammin
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Moryachkov R., Tomilin F. N., Shchugoreva I., Spiridonova V., Peters G., Zabluda V. N.
Заглавие : Small angle X-ray scattering and atomic structure of aptamer biomolecules
Коллективы : Federal Research Center KSC SB RAS, Kirensky Institute of Physics, Siberian Federal Univercity, International workshop on actual problems of condensed matter physics
Место публикации : Int. workshop on actual probl. of cond. matt. phys.: Program. Book of abstracts/ Fed. Res. Center KSC SB RAS, Kirensky Inst. of phys., Sib. Fed. Univ. - Krasnoyarsk, 2017. - P.14-15
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stolyar S. V., Li O. A., Nikolaeva E. D., Vorotynov A. M., Velikanov D. A., Knyazev Yu. V., Bayukov O. A., Iskhakov R. S., Kryukova O. V., Pyankov V. F., Volochaev M. N., Mokhov A. A.
Заглавие : Preparation and properties of magnetic composites γ-Fe2O3/SiO2/aptamer(FAS9) for magnetic resonance hyperthermia
Колич.характеристики :8 с
Место публикации : Phys. Met. Metallogr. - 2023. - Vol. 124, Is. 14. - P.1689-1696. - ISSN 0031918X (ISSN), DOI 10.1134/S0031918X23601439. - ISSN 15556190 (eISSN)
Примечания : Cited References: 27
Аннотация: Powders of maghemite γ-Fe2O3 with an average diameter of 8 nm, γ-Fe2O3/SiO2 composites with an agglomerate diameter of about 50 nm and a size of interspersed γ-Fe2O3 particles of 6 nm, and γ‑Fe2O3/SiO2/aptamer(FAS9) composites were synthesized by chemical deposition. Mössbauer spectra were measured, the static and dynamic magnetic properties of the powders were studied, and the coercive force was determined, which decreases from 14 Oe for γ-Fe2O3 powders to 3 Oe for the γ-Fe2O3/SiO2 composite. It is shown that the particle blocking temperature is close to room temperature. The increment of temperature of the powders was measured in the ferromagnetic resonance mode; the temperature of the Fe2O3/SiO2 composite (ΔT ≈ 16°C) turned out to be higher than that of the pure γ-Fe2O3 powder (ΔT ≈ 10°C). It has been experimentally shown that temperature increment ΔT is proportional to the square of the microwave field amplitude. It has been shown that the composition γ-Fe2O3/SiO2/aptamer FAS9 is able to effectively bind to tumor cells, and FMR hyperthermia leads to a decrease in the viability of tumor cells.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kostyukov A. S., Isaev I. L., Ershov A. E., Gerasimov V. S., Polyutov S. P., Karpov S. V.
Заглавие : Part II. Nanobubbles around plasmonic nanoparticles in terms of modern simulation modeling: what makes them kill the malignant cells?
Коллективы : Ministry of Science and High Education of Russian Federation [FSRZ-2020-0008]; RFBRRussian Foundation for Basic Research (RFBR); Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science [20-42-240003]
Место публикации : J. Phys. D. - 2022. - Vol. 55, Is. 17. - Ст.175402. - ISSN 0022-3727, DOI 10.1088/1361-6463/ac4c1f. - ISSN 1361-6463(eISSN)
Примечания : Cited References: 49. - The research was supported by the Ministry of Science and High Education of Russian Federation (Project No. FSRZ-2020-0008), and was funded by RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, Project No. 20-42-240003
Предметные рубрики: STRESS WAVES
LASER
MEMBRANE
DAMAGE
DEATH
LYSIS
Аннотация: We have established numerically the physical pattern and conditions for formation of nanosized bubbles in aqueous medium around biocompatible plasmonic nanoparticles (NPs) selectively bound to the membrane of the malignant cells by means of DNA-aptamers under the action of picosecond laser radiation. The results obtained are based on the finite volume method and hydrodynamic models underlying the ANSYS Fluent package with extended capabilities. We have found the main features and previously unknown dominant factors of the damage effect on the cell membrane at the moment of the bubble nucleation around the plasmonic NPs of different types taking into account the influence of the closely located membrane. Information on the kinetics of spatial distribution of pressure, temperature and the relative proportion of vapor in the 'nanoparticle-membrane-medium' system have been obtained. The attention is drawn to the advantages of using biocompatible, perfectly absorbing core–shell plasmonic NPs for anti-tumor therapy characterized by an increased mechanical effect on malignant cell membranes at lower laser radiation intensity and the spectral position of their plasmon resonance (λ = 700 nm) in the hemoglobin transparency range. This ensures penetration of laser radiation deep into tissues. The paper is provided with an extensive review of key publications and the state-of-art in this area.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zamay T. N., Starkov A. K., Kolovskaya O. S., Zamay G. S., Veprintsev D. V., Luzan N., Nikolaeva E. D., Lukyanenko K. A., Artyushenko P. V., Shchugoreva I. A., Glazyrin Y. E., Koshmanova A. A., Krat A. V., Tereshina D. S., Zamay S. S., Pats Y. S., Zukov R. A., Tomilin F. N., Berezovski M. V., Kichkailo A. S.
Заглавие : Nucleic acid aptamers increase the anticancer efficiency and reduce the toxicity of cisplatin-arabinogalactan conjugates in vivo
Место публикации : Nucleic Acid Ther. - 2022. - Vol. 32, Is. 6. - P.497-506. - ISSN 21593337 (ISSN), DOI 10.1089/nat.2022.0024. - ISSN 21593345 (eISSN)
Примечания : Cited References: 42
Аннотация: Cisplatin is an effective drug for treating various cancer types. However, it is highly toxic for both healthy and tumor cells. Therefore, there is a need to reduce its therapeutic dose and increase targeted bioavailability. One of the ways to achieve this could be the coating of cisplatin with polysaccharides and specific carriers for targeted delivery. Nucleic acid aptamers could be used as carriers for the specific delivery of medicine to cancer cells. Cisplatin-arabinogalactan-aptamer (Cis-AG-Ap) conjugate was synthesized based on Cis-dichlorodiammineplatinum, Siberian larch arabinogalactan, and aptamer AS-42 specific to heat-shock proteins (HSP) 71?kDa (Hspa8) and HSP 90-beta (Hsp90ab1). The antitumor effect was estimated using ascites and metastatic Ehrlich tumor models. Cis-AG-Ap toxicity was assessed by blood biochemistry on healthy mice. Here, we demonstrated enhanced anticancer activity of Cis-AG-Ap and its specific accumulation in tumor foci. It was shown that targeted delivery allowed a 15-fold reduction in the therapeutic dose of cisplatin and its toxicity. Cis-AG-Ap sufficiently suppressed the growth of Ehrlich's ascites carcinoma, the mass and extent of tumor metastasis in vivo. Arabinogalactan and the aptamers promoted cisplatin efficiency by enhancing its bioavailability. The described strategy could be very promising for targeted anticancer therapy.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zamay T. N., Zamay G. S., Belyanina I. V., Zamay S. S., Denisenko V. V., Kolovskaya O. S., Ivanchenko T. I., Grigorieva V. L., Garanzha I. V., Veprintsev D. V., Glazyrin Y. E., Shabanov A. V., Prinz V., Seleznev V. A., Sokolov A. E., Prokopenko V. S., Kim P. D., Gargaun A., Berezovski M. V., Zamay A. S.
Заглавие : Noninvasive microsurgery using aptamer-functionalized magnetic microdisks for tumor cell eradication
Место публикации : Nucl. Acid Therapeutics. - 2017. - Vol. 27, No. 2. - P.105-114. - DOI 10.1089/nat.2016.0634
Примечания : Cited References: 18
Ключевые слова (''Своб.индексиров.''): aptamer--magnetic microdisks--magnetodynamic therapy--tumor eradication
Аннотация: Magnetomechanical cell disruption using nano- and microsized structures is a promising biomedical technology used for noninvasive elimination of diseased cells. It applies alternating magnetic field (AMF) for ferromagnetic microdisks making them oscillate and causing cell membrane disruption with cell death followed by apoptosis. In this study, we functionalized the magnetic microdisks with cell-binding DNA aptamers and guided the microdisks to recognize cancerous cells in a mouse tumor in vivo. Only 10 min of the treatment with a 100 Hz AMF was enough to eliminate cancer cells from a malignant tumor. Our results demonstrate a good perspective of using aptamer-modified magnetic microdisks for noninvasive microsurgery for tumors.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolovskaya, Olga S., Zyuzyukina, Alena V., Dassie, Justin P., Zamay, Galina S., Zamay, Tatiana N., Boyakova, Nina V., Khorzhevskii, Vladimir A., Kirichenko, Daria A., Lapin I. N., Shchugoreva, Irina A., Artyushenko, Polina V., Tomilin F. N., Veprintsev, Dmitry V., Glazyrin, Yury E., Minic, Zoran, Bozhenko, Vladimir K., Kudinova, Elena A., Kiseleva, Yana Y., Krat, Alexey V., Slepov, Eugene V., Bukatin, Anton S., Zukov, Ruslan A., Shesternya, Pavel A., Berezovski, Maxim V., Giangrande, Paloma H., Kichkailo, Anna S.
Заглавие : Monitoring of breast cancer progression via aptamer-based detection of circulating tumor cells in clinical blood samples
Место публикации : Front. Mol. Biosci. - 2023. - Vol. 10. - Ст.1184285. - ISSN 2296889X (eISSN), DOI 10.3389/fmolb.2023.1184285
Примечания : Cited References: 37. - CTCs isolation method development and clinical sample analyses were supported by the Ministry of Healthcare of Russian Federation project REYC-2023-0012. The Ministry of Science and Higher Education of the Russian Federation project FWES-2022-0005 supported aptamer characterization, molecular modeling. MDA-231/LUC cells were from R. Domann (Jenkin et al. Breast Cancer Research, 2005). Technical and instrumental support was provided by the Shared Core Facilities of Molecular and Cell Technologies at Krasnoyarsk State Medical University and Krasnoyarsk Regional Centre for Collective Use at the Federal Research Centre “KSC SB RAS.” The confocal fluorescence microscopy research was carried out with the equipment of the Tomsk Regional Core Shared Research Facilities Center of the National Research Tomsk State University. The Center was supported by the Ministry of Science and Higher Education of the Russian Federation, grant no. 075-15-2021-693 (No. 13.RFC.21.0012)The authors are grateful to all the patients and hospital staff participating in this research. We acknowledge the assistance of the AptamerLab LCC (www.aptamerlab.com) and personally Vasily Mezko for the technical support. The authors thank Valentina L. Grigoreva, and Irina V. Gildebrand for the help with СTC staining
Аннотация: Introduction: Breast cancer (BC) diagnostics lack noninvasive methods and procedures for screening and monitoring disease dynamics. Admitted CellSearch® is used for fluid biopsy and capture of circulating tumor cells of only epithelial origin. Here we describe an RNA aptamer (MDA231) for detecting BC cells in clinical samples, including blood. The MDA231 aptamer was originally selected against triple-negative breast cancer cell line MDA-MB-231 using cell-SELEX. Methods: The aptamer structure in solution was predicted using mFold program and molecular dynamic simulations. The affinity and specificity of the evolved aptamers were evaluated by flow cytometry and laser scanning microscopy on clinical tissues from breast cancer patients. CTCs were isolated form the patients’ blood using the developed method of aptamer-based magnetic separation. Breast cancer origin of CTCs was confirmed by cytological, RT-qPCR and Immunocytochemical analyses. Results: MDA231 can specifically recognize breast cancer cells in surgically resected tissues from patients with different molecular subtypes: triple-negative, Luminal A, and Luminal B, but not in benign tumors, lung cancer, glial tumor and healthy epithelial from lungs and breast. This RNA aptamer can identify cancer cells in complex cellular environments, including tumor biopsies (e.g., tumor tissues vs. margins) and clinical blood samples (e.g., circulating tumor cells). Breast cancer origin of the aptamer-based magnetically separated CTCs has been proved by immunocytochemistry and mammaglobin mRNA expression. Discussion: We suggest a simple, minimally-invasive breast cancer diagnostic method based on non-epithelial MDA231 aptamer-specific magnetic isolation of circulating tumor cells. Isolated cells are intact and can be utilized for molecular diagnostics purposes.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lupu, Loredana, Wiegand, Pascal, Huttmann N., Rawer, Stephan, Kleinekofort, Wolfgang, Shugureva, Irina, Kichkailo, Anna S., Tomilin F. N., Lazarev, Alexander, Berezovski, Maxim V., Przybylski, Michael
Заглавие : Molecular epitope determination of aptamer complexes of the multidomain protein C-met by proteolytic affinity-mass spectrometry
Коллективы : LOEWE-3 Funding Agency, Hessen-Agentur, Wiesbaden, Germany [696/19-16]
Место публикации : ChemMedChem. - 2020. - Vol. 15, Is. 4. - P.363-369. - ISSN 1860-7179, DOI 10.1002/cmdc.201900489. - ISSN 1860-7187(eISSN)
Примечания : Cited References: 40. - We gratefully acknowledge the advice and assistance of Prof. Friedemann Volklein and Oliver Muller, MSc in the preparation of chips for the SPR affinity determinations. We thank Dr. Stefan Maeser, Biogen GmbH, Munchen, for valuable advice and critical reading of the manuscript. This work has been partially funded (Chip-MS epitope analysis) by the LOEWE-3 Funding Agency, Hessen-Agentur, Wiesbaden, Germany; Grant 696/19-16
Предметные рубрики: DNA APTAMERS
ANTIBODIES
RECOGNITION
Аннотация: C‐Met protein is a glycosylated receptor tyrosine kinase of the hepatocyte growth factor (HGF), composed of an α and a β chain. Upon ligand binding, C‐Met transmits intracellular signals by a unique multi‐substrate docking site. C‐Met can be aberrantly activated leading to tumorigenesis and other diseases, and has been recognized as a biomarker in cancer diagnosis. C‐Met aptamers have been recently considered a useful tool for detection of cancer biomarkers. Herein we report a molecular interaction study of human C‐Met expressed in kidney cells with two DNA aptamers of 60 and 64 bases (CLN0003 and CLN0004), obtained using the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure. Epitope peptides of aptamer‐C‐Met complexes were identified by proteolytic affinity‐mass spectrometry in combination with SPR biosensor analysis (PROTEX‐SPR‐MS), using high‐pressure proteolysis for efficient digestion. High affinities (KD, 80–510 nM) were determined for aptamer‐C‐Met complexes, with two‐step binding suggested by kinetic analysis. A linear epitope, C‐Met (381–393) was identified for CLN0004, while the CLN0003 aptamer revealed an assembled epitope comprised of two peptide sequences, C‐Met (524–543) and C‐Met (557–568). Structure modeling of C‐Met‐aptamers were consistent with the identified epitopes. Specificities and affinities were ascertained by SPR analysis of the synthetic epitope peptides. The high affinities of aptamers to C‐Met, and the specific epitopes revealed render them of high interest for cellular diagnostic studies.
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16.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Shchugoreva I., Moryachkov R., Zabluda V. N., Sokolov A. E., Spiridonova V. A., Gruzinov A., Zamay A. S., Veprincev D., Zamay G. S., Tomilin F. N., Ovchinnikov S. G.
Заглавие : Molecular design of aptamer structure by computer simulations
Коллективы : Euro-Asian Symposium "Trends in MAGnetism", "Trends in MAGnetism", Euro-Asian Symposium, Институт физики им. Л.В. Киренского Сибирского отделения РАН
Место публикации : VI Euro-Asian Symposium "Trends in MAGnetism" (EASTMAG-2016): abstracts/ ed.: O. A. Maksimova, R. D. Ivantsov. - Krasnoyarsk: KIP RAS SB, 2016. - Ст.P12.2. - P.558. - ISBN 978-5-904603-06-9 (Шифр -478014040)
Примечания : We acknowledge the grant by the Ministry of Education and Science (Agreement No.14.607.21.0104 (RFMEFI60714X0104)) for the financial support of this work
Ключевые слова (''Своб.индексиров.''): dna--selex--saxs--qm/mm--aptamer--g-quartets
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17.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Sokolov A. Е., Zabluda V. N., Sherepa A. V., Knyazev Yu. V., Volochaev M. N., Kurilina A., Velikanov D. A., Goncharova D. A., Shabalina A., Svetlichnyi V.
Заглавие : Magnetic properties of iron oxide nanoparticles to create aptamer bionanoconjugates
Коллективы : Aptamers in Russia, international conference
Место публикации : Aptamers in Russia, international conference (1 ; 2019 ; Aug. 27-30 ; Krasnoyarsk). Molecular Therapy - Nucleic Acids: book of abstracts of the 1st Int. conf. "Aptamers in Russia 2019". - 2019. - Vol. 17, Suppl. 1. - P.12
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stolyar S. V., Chekanova L. A., Yaroslavtsev R. N., Komogortsev S. V., Gerasimova Yu. V., Bayukov O. A., Volochaev M. N., Iskhakov R. S., Garanzha I. V., Kolovskaya O. S., Bairmani M. Sh., Zamay T. N.
Заглавие : Magnetic nanoconstructions of iron oxides coated with arabinogalactan functionalized with DNA aptamer
Коллективы : International Scientific Conference "Conference on Applied Physics, Information Technologies and Engineering"
Место публикации : J. Phys. Conf. Ser. - 2019. - Vol. 1399. - Ст.022026. - ISSN 1742-6588, DOI 10.1088/1742-6596/1399/2/022026. - ISSN 1742-6596 (eISSN)
Примечания : Cited References: 6
Предметные рубрики: Applied Physics
Аннотация: New composite nanoparticles for biomedical applications have been manufactured. The particles consist of an anisomeric magnetite core coated with arabinogalactan and are functionalized with cameras for As-14 ascites cells (Ehrlich carcinoma). The binding of ascitic Ehrlich carcinoma cells to magnetic nanoparticles was evaluated using fluorescence microscopy.
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19.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Zamay G. S., Zamay T., Kolovsky V., Shabanov A. V., Kolovskaya O. S., Krat A. V., Modestov A., Sokolov A. E., Chetvergov N. A., Gargaun A., Berezovski M., Zamay S. S., Zamay A. S., Volochaev M. N., Svetlichnyi V., Lapin I. N., Shabalina A.
Заглавие : Magnetic beads increase the detection limit of aptamer-based electrochemical sensor
Коллективы : Euro-Asian Symposium "Trends in MAGnetism", "Trends in MAGnetism", Euro-Asian Symposium, Институт физики им. Л.В. Киренского Сибирского отделения РАН
Место публикации : VI Euro-Asian Symposium "Trends in MAGnetism" (EASTMAG-2016): abstracts/ ed.: O. A. Maksimova, R. D. Ivantsov. - Krasnoyarsk: KIP RAS SB, 2016. - Ст.P12.5. - P.562. - ISBN 978-5-904603-06-9 (Шифр -478014040)
Примечания : This work was supported by Ministry of Education and Science of Russian Federation Federal Target Program # 14.604.21.0105 (RFMEFI60414X0105)
Ключевые слова (''Своб.индексиров.''): magnetic beads--protein--aptamer--electrochemical sensor
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20.

Вид документа : Однотомное издание
Шифр издания :
Заглавие : International workshop on actual problems of condensed matter physics : Program. Book of abstracts
Выходные данные : Krasnoyarsk, 2017
Колич.характеристики :30 с
Коллективы : Federal Research Center KSC SB RAS, Kirensky Institute of Physics, Siberian Federal Univercity, International Workshop on Actual Problems of Condensed Matter Physics (27 Mar. - 1 Apr. 2017; Krasnoyarsk/ Cheremushki) :
Содержание : Magnetic and transport properties of the epitaxial Fe3Si film on a Si substrate/ I. A. Bondarev. The magnetic anisotropy of the Fe and Fe(1-x)Si(x) thin films depend on/ I. A. Yakovlev [и др.]. Inverted opals as the Josephson networks of weak links/ S. I. Popkov [и др.]. Electronic structure and Fermi surface within the cluster perturbation theory in X-operators representation/ S. Nikolaev, V. I. Kuz'min, S. G. Ovchinnikov. DFT investigation of electronic and optical magnetic properties of one dimensional transition metal halide structuresTmHaI3/ A. S. Fedorov [и др.]. Effect of interatomic exchange interaction on spin crossover and Mott-Hubbard transition under high pressure and the physical properties of the low Earth’s mantle/ S. G. Ovchinnikov [и др.]. Extremely high magnetic-field sensitivity of charge transport in the Mn/SiO2/p-Si hybrid structure/ I. A. Tarasov [и др.]. Marnetic-field sensitivity of charge transport in silicon-based hybrid structures/ N. V. Volkov [et al.]. Fabrication of multi-terminal planar devices based on epitaxial Fe1-xSix films grown on Si(111)/ A. V. Lukyanenko, A. S. Tarasov, I. A. Tarasov [et al.] ; A. V. Luyanenko [и др.]. Magnetic field-driven lateral photovoltaic effect in the Fe/SiO2/p-Si hibrid structure with the Scottky barrier/ M. V. Rautskii [и др.]. Small angle X-ray scattering and atomic structure of aptamer biomolecules/ R. Moryachkov [и др.]. Iron silicides and pure iron epitaxial and highly-textured nanostructures on silicon: growth and their physical properties/ I. A. Tarasov [и др.]. Magnetic nanoparticles and DNA-aptamers conjugates for diagnostics and therapy of cancer/ A. E. Sokolov [и др.]. The microscopic origin of ferromagnetism in Fe silicides/ I. S. Sandalov [и др.].
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