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1.
Biodegradation of
polyhydroxyalkanoates
by soil microbial communities of different structures and detection of PHA degrading microorganisms / A. N. Boyandin [et al.]> // Appl. Biochem. Microbiol. - 2012. -
Vol. 48
,
Is. 1
. - P. 28-36,
DOI
10.1134/S0003683812010024. - Cited References: 39. - The work was supported by the project initiated by the Government of the Russian Federation for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (Agreement No. 11.G34.31.0013) and the Program of Integrated Research of the Presidium of the Siberian Branch of Russian Academy of Sciences (project no. 96). . - ISSN 0003-6838
РУБ
Biotechnology & Applied Microbiology + Microbiology
Рубрики:
POLY-BETA-HYDROXYBUTYRATE
CHAIN-LENGTH
POLYHYDROXYALKANOATES
DEGRADATION
FILMS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
BACTERIA
ACID
Аннотация:
Biodegradation of microbial linear polymers of hydroxyalkanoic acids (
polyhydroxyalkanoates
, PHAs) by soil microbial communities of different structures has been studied during two field seasons in different weather conditions. This process was shown to be influenced by the polymer chemical composition, temperature, humidity, and the microbial soil component. The PHA degradation was accompanied by a decrease in the polymer molecular weight and an increase in the degree of crystallinity, indicating the preferential destruction of the amorphous phase compared to the crystalline one. The quantity of the true PHA destructors developing at the surface of the polymer samples was lower than the quantity of accompanying bacteria. The dominant PHA degrading microorganisms under the test conditions were identified as bacteria of the genera Variovorax, Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus, and Xanthomonas and as micromycetes from Penicillium, Paecilomyces, Acremonium, Verticillium, and Zygosporium.
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Публикация на русском языке
Биодеградация полигидроксиалканоатов почвенными микробиоценозами различной структуры и выявление микроорганизмов-деструкторов [Текст] / А. Н. Бояндин [и др.] // Прикл. биохимия и микробиол. : Наука, 2012. - Т. 48 № 1. - С. 35-44
Держатели документа:
[Boyandin, A. N.
Volova, T. G.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Prudnikova, S. V.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Filipenko, M. L.
Khrapov, E. A.] Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia
[Vasil'ev, A. D.] Russian Acad Sci, Siberian Branch, Kirenskii Inst Phys, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Boyandin, A. N.; Бояндин, Анатолий Николаевич; Prudnikova, S. V.; Filipenko, M. L.; Khrapov, E. A.; Vasil'ev, A. D.; Васильев, Александр Дмитриевич; Volova, T. G.; Волова, Татьяна Григорьевна
}
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2.
Fundamental basis of
production and application of biodegradable
polyhydroxyalkanoates
/ T. G. Volova [et al.]> // J. Sib. Fed. Univ. Biol. - 2012. -
Vol. 5
,
Is. 3
. - P. 280-299 ; Журн. СФУ. Сер. "биология"
Перевод заглавия:
Фундаментальные основы производства и применения биодеградируемых полигидроксиалканоатов
Кл.слова (ненормированные):
biopolymers
--
polyhydroxyalkanoates
--
substrates
--
synthesis
--
properties
--
processing
--
biomedical applications
--
биополимеры
--
полигидроксиалканоаты
--
субстраты
--
синтез
--
свойства
--
обработка
--
медико-биологическое применение
Аннотация:
Microbial
polyhydroxyalkanoates
(PHAs) have long been studied due to their potential for replacement of petroleum-based plastic. The study addresses the effect of different conditions of carbon nutrition on synthesis of
polyhydroxyalkanoates
by the bacterium Ralstonia eutropha. First and foremost, PHAs are carbon storage compounds for many organisms. There are still many aspects of the physiology of PHA accumulation and degradation that are still not understood. Measurements of key P3HBrelated enzyme activities throughout cell growth reveal correlations of acetoacetyl-CoA reductase and synthase enzyme activity maxima with P3HB biosynthesis. The investigation addressed kinetic parameters of growth and accumulation of
polyhydroxyalkanoates
and gas exchange parameters of the culture of the CO-resistant strain of the hydrogen bacteria Ralstonia eutropha B 5786 cultivated on synthesis gas - a product of gasification of brown coals. The results were compared with those obtained by growing the bacteria on electrolytic hydrogen and it was concluded that synthesis gas can be successfully used to produce PHAs. In experiments with wild-type strain it has been first found that under mixotrophic growth conditions - CO2 + co-substrate (alcanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chain-length monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize multi-component PHAs with 3-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate as major monomers and 3-hydroxyheptanoate and 3-hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (3-hydroxyhexanoate and 3-hydroxyoctanoate) but also 3-hydroxyvalerate, making possible synthesis of four-component PHAs, containing 3-hydroxybutyrate and 3-hydroxyhexanoate as major components. A family of short- and medium-chain-length four- and five-component PHAs has been synthesized and their physicochemical and biomedical-properties examined.
Полигидроксиалканоаты (ПГА) - перспективные материалы, которые могут заменить пластмассы на углеводородной основе, и они исследуются уже в течение длительного времени. Данная работа рассматривает воздействие различных условий углеродного питания на синтез полигидроксиалканоатов бактериями Ralstonia eutropha. Прежде всего ПГА являются запасными веществами для многих организмов. Многие аспекты физиологии накопления и разрушения ПГА остаются пока не изученными. Измерение активности ключевых ферментов 3ПГБ на протяжении роста клетки показывает связь максимумов активности ацетоацетил-КоА редуктазы и синтазы с биосинтезом 3ПГБ. Были исследованы кинетические параметры роста и накопления полигидроксиалканоатов и параметры газообмена культуры штамма водородных бактерий Ralstonia eutropha B 5786, устойчивого к СО, при культивировании на синтез-газе - продукте газификации бурых углей. Проведено сравнение полученных результатов с данными, полученными при выращивании бактерий на электролитическом водороде, и показано, что синтез-газ может успешно применяться для получения ПГА. В экспериментах с природным штаммом бактерий впервые было показано, что при миксотрофных условиях роста - CO2 + дополнительный субстрат (алкановые кислоты) - бактерии способны синтезировать многокомпонентные ПГА, состоящие из коротко- и среднецепочечных мономеров с длиной цепи от 4 до 8 атомов углерода. Показано, что состав ПГА зависит от типа дополнительного субстрата. Жирные кислоты с нечётным количеством атомов углерода стимулируют бактерии синтезировать многокомпонентные ПГА, состоящие из таких основных компонентов, как 3-гидроксибутират, 3-гидроксивалерат и 3-гидроксигексаноат, и второстепенных, эпизодических компонентов: 3-гидроксигептаноата и 3-гидроксиоктаноата. Жирные кислоты с чётным количеством атомов углерода стимулируют синтез как соответствующих им мономеров (3-гидроксигексаноата и 3-гидроксиоктаноата), так и 3-гидроксивалерата, что позволяет получать четырёхкомпонентные ПГА, содержащие 3-гидроксибутират и 3-гидроксигексаноат в качестве основных компонентов. Было синтезировано семейство коротко- и среднецепочечных четырёх- и пятикомпонентных ПГА и проведено исследование их физико-химических и медико-биологических свойств.
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Доп.точки доступа:
Volova, T. G.; Волова, Татьяна Григорьевна; Shishatskaya, E. I.; Шишацкая, Екатерина Игоревна; Zhila, N. O.; Жила, Наталья Олеговна; Киселев, Евгений Геннадьевич; Kiselev, E. G.; Mironov, P. V.; Vasil'ev, A. D.; Васильев, Александр Дмитриевич; Peterson, I. V.; Петерсон, Иван Викторович; Sinskey, A. J.
}
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3.
The physicochemical properties
of
polyhydroxyalkanoates
with different chemical structures / T. G. Volova [et al.]> // Polym. Sci. Ser. A. - 2013. -
Vol. 55
,
Is. 7
. - P. 427-437,
DOI
10.1134/S0965545X13070080. - Cited References: 41 . - 11. - ISSN 0965-545X
Перевод заглавия:
Физико-химические свойства полигидроксиалканоатов с разной химической структурой
РУБ
Polymer Science
Рубрики:
MICROBIAL SYNTHESIS
RALSTONIA-EUTROPHA
COMAMONAS-ACIDOVORANS
AEROMONAS-HYDROPHILA
POLY(3-HYDROXYBUTYRATE-CO-4-HYDROXYBUTYRATE)
BIOSYNTHESIS
COPOLYMERS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE)
POLYMERS
Аннотация:
A set of
polyhydroxyalkanoates
are synthesized, and a comparative study of their physicochemical properties is performed. The molecular masses and polydispersities of
polyhydroxyalkanoates
are found to be independent of their chemical structures. It is shown that the temperature characteristics and degrees of crystallinity of
polyhydroxyalkanoates
are affected by the chemical compositions of the monomers and their quantitative contents in the polymers. The incorporation of 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate units into the chain of poly(3-hydroxybutyrate) decreases its melting point and thermal degradation temperature relative to these parameters of a homogeneous poly(3-hydroxybutyrate) sample (175 +/- 5A degrees C and 275 +/- 5A degrees C, respectively). The higher the content of the second monomer units in the poly(3-hydroxybutirate) chain, the greater the changes. The degrees of crystallinity of polyhydroxyalkanoate copolymers are generally lower than that of poly(3-hydroxybutyrate) (75 +/- 5%). The effect on the ratio of the amorphous and crystalline phases of the copolymer samples becomes more pronounced in the series 3-hydroxy-valerate-3-hydroxyhexanoate-4-hydroxybutyrate. The prepared samples exhibit different properties ranging from rigid thermoplastic materials to engineering elastomers.
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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia
Siberian State Technol Univ, Krasnoyarsk 660049, Russia
Russian Acad Sci, Siberian Branch, LV Kirensky Phys Inst, Krasnoyarsk 660036, Russia
MIT, Cambridge, MA 02139 USA;
Доп.точки доступа:
Volova, T. G.; Волова, Татьяна Григорьевна; Zhila, N. O.; Жила, Наталья Олеговна; Shishatskaya, E. I.; Шишацкая, Екатерина Игоревна; Mironov, P. V.; Vasil'ev, A. D.; Васильев, Александр Дмитриевич; Sukovatyi, A. G.; Суковатый, Алексей Григорьевич; Sinskey, A. J.
}
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4.
Cell growth and
accumulation of
polyhydroxyalkanoates
from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646 / T. G. Volova [et al.]> // Bioresource Technol. - 2013. -
Vol. 146
. - P. 215-222,
DOI
10.1016/j.biortech.2013.07.070 . - ISSN 0960-8524
Перевод заглавия:
Рост клеток и накопление полигидроксиалканоатов из CO2 и H2 водород-окисляющими бактериями, Cupriavidus eutrophus B-10646
Кл.слова (ненормированные):
Autotrophic synthesis
--
Cupriavidus eutrophus
--
Polyhydroxyalkanoates
Аннотация:
Synthesis of
polyhydroxyalkanoates
(PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h-1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h-1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, ?-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. В© 2013 Elsevier Ltd.
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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Siberian Fed Univ, Krasnoyarsk, Russia
Russian Acad Sci, Siberian Branch, LV Kirensky Phys Inst, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Krasnoyarsk 660036, Russia;
Доп.точки доступа:
Volova, T. G.; Волова, Татьяна Григорьевна; Kiselev, E. G.; Киселев, Евгений Геннадьевич; Shishatskaya, E. I.; Шишацкая, Екатерина Игоревна; Zhila, N. O.; Жила, Наталья Олеговна; Boyandin, A. N.; Бояндин, Анатолий Николаевич; Syrvacheva, D. A.; Vinogradova, O. N.; Kalacheva, G. S.; Калачева, Галина Сергеевна; Vasiliev, A. D.; Васильев, Александр Дмитриевич; Peterson, I. V.; Петерсон, Иван Викторович
}
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5.
Физико-химические свойства полигидроксиалканоатов
различного химического строения / Т. Г. Волова [и др.]> // Высокомолекулярные соединения. Серия А. - 2013. -
Т. 55
,
№ 7
. - С. 775-786,
DOI
10.7868/S0507547513070210 . - ISSN 0507-5475
Аннотация:
Синтезирован ряд полигидроксиалканоатов и проведено сравнительное исследование их физико-химических свойств. Не обнаружено зависимости величины молекулярной массы и полидисперсности полигидроксиалканоатов от их химического строения. Показано, что на температурные характеристики и степень кристалличности полигидроксиалканоатов влияют химический состав мономеров и их количественное содержание в полимере. Включение в цепь поли(3-гидроксибутиратата) звеньев 4-гидроксибутирата, 3-гидроксивалерата или 3-гидроксигексаноата понижает температуры плавления и термической деградации относительно этих величин у гомогенного поли(3-гидроксибутирата) (175 ± 5°С и 275 ± 5°С соответственно). Эти изменения тем более значительны, чем выше содержание в цепи поли(3-гидроксибутирата) звеньев второго мономера. Степень кристалличности сополимерных полигидроксиалканоатов в целом ниже, чем у поли(3-гидроксибутирата) (75 ± 5%). Выраженность влияния на соотношение аморфной и кристаллических фаз у сополимерных образцов усиливается в ряду 3-гидроксивалерат-3-гидроксигексаноат-4-гидроксибутират. Получены образцы с различными свойствами: от жестких термопластов до конструкционных эластомеров.
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Переводная версия
The physicochemical properties of
polyhydroxyalkanoates
with different chemical structures // Polymer Science Series A : MAIK Nauka-Interperiodica / Springer, 2013. - Vol. 55, Is. 7. - P.427-437. - 11. - ISSN 0965-545X
Держатели документа:
Институт физики им. Л.В. Киренского СО РАН
Доп.точки доступа:
Волова, Татьяна Григорьевна; Volova, T. G.; Жила, Наталья Олеговна; Шишацкая, Екатерина Игоревна; Миронов, П. В.; Васильев, Александр Дмитриевич; Vasiliev A. D.; Суковатый, Алексей Григорьевич; Sinskey, A. J.
}
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6.
Electrospinning of polyhydroxyalkanoate
fibrous scaffolds: effects on electrospinning parameters on structure and properties / T. . Volova [et al.]> // J. Biomater. Sci.-Polym. Ed. - 2014. -
Vol. 25
,
Is. 4
. - P. 370-393,
DOI
10.1080/09205063.2013.862400. - Cited References: 52. - This study was financially supported by Project 'Biotechnologies of novel biomaterials: innovative biopolymers and devices for biomedicine' (Agreement No. 1 of 15.02.2013 to Agreement No. 11.G34.31.0013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, 'On measures designed to attract leading scientists to the Russian institutions of higher learning' and Grant of the RF President for supporting young Doctors of Sciences No. MD-3112.2012.4. . - ISSN 0920-5063. - ISSN 1568-5624
РУБ
Engineering, Biomedical + Materials Science, Biomaterials + Polymer Science
Рубрики:
TISSUE ENGINEERING APPLICATIONS
FIBER MATS
POLY 3-HYDROXYBUTYRATE
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
BIOCOMPATIBILITY
PROLIFERATION
FABRICATION
NANOFIBERS
COPOLYMERS
MEMBRANES
Кл.слова (ненормированные):
electrospinning
--
polyhydroxyalkanoates
--
ultrafine fibers
--
physical-mechanical properties
--
fibroblast cells
Аннотация:
IIn this study, electrospinning was used to prepare ultrafine fibers from PHAs with different chemical compositions: P(3HB) and copolymers: P(3HB-co-4HB), P(3HB-co-3HV), and P(3HB-co-3HHx). The main process parameters that influence ultrafine fiber diameter and properties (polymer concentration, solution feeding rate, working distance, and applied voltage) have been investigated and their effects evaluated. The study revealed electrospinning parameters for the production of high-quality ultrafine fibers and determined which parameters should be varied to tailor the properties of the products. This study is the first to compare biological and physical-mechanical parameters of PHAs with different chemical compositions as dependent upon the fractions of monomers constituting the polymers and ultrafine fiber orientation. Mechanical strength of aligned ultrafine fibers prepared from different PHAs is higher than that of randomly oriented ones; no significant effect of ultrafine fiber orientation on surface properties has been found. None of the fibrous scaffolds produced by electrospinning from PHAs had any adverse effects on attachment, growth, and viability of NIH 3T3 mouse fibroblast cells, and all of them were found to be suitable for tissue engineering applications.
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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia
Russian Acad Sci, LV Kirenskii Inst Phys, Siberian Branch, Krasnoyarsk 660036, RussiaИФ СО РАН;
Доп.точки доступа:
Volova, T.; Волова, Татьяна Григорьевна; Goncharov, D.; Sukovatyi, A. G.; Суковатый, Алексей Григорьевич; Shabanov, A. V.; Шабанов, Александр Васильевич; Nikolaeva, E.; Shishatskaya, E.; Project 'Biotechnologies of novel biomaterials: innovative biopolymers and devices for biomedicine' [1, 11.G34.31.0013]; Government of the Russian Federation [220]; RF President for supporting young Doctors of Sciences [MD-3112.2012.4]
}
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7.
Short-term culture of
monocytes as an in vitro evaluation system for bionanomaterials designated for medical use / E. I. Shishatskaya [et al.]> // Food Chem. Toxicol. - 2016. -
Vol. 96
. - P. 302-308,
DOI
10.1016/j.fct.2016.08.025. - Cited References: 46 . - ISSN 0278-6915
РУБ
Food Science & Technology + Toxicology
Рубрики:
MAGNETIC NANOPARTICLES
CELLULAR REDUCTION
PROTEIN CORONA
CELLS
MECHANOTRANSDUCTION
NANOMATERIALS
LOCALIZATION
BIOMATERIALS
CYTOSKELETON
ACTIVATION
Кл.слова (ненормированные):
Biocompatible biopolymers
--
Polyhydroxyalkanoates
--
Nanomaterials
--
Nanodiamonds
--
Fullerenes
--
Bio-nanomaterials
--
Monocytes
Аннотация:
We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the viability/metabolic activity of cells. The scanning electron microscopy assay can detect fine changes in cell morphology. In the present study BNM have been shown to affect; in a size, chemical composition and morphological characteristics-dependent manner, the ability of monocytes to reduce MTT as well as their morphology. Moreover, the specific effects of ND and F on MTT reduction and cell morphology were exhibited in a dose-dependent manner and sensitive to the formation of surface protein corona. Our results suggest that short-term culture of monocytes is a sensitive model system for assessing the biological effects of BMPs in vitro. © 2016 Elsevier Ltd
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Держатели документа:
Siberian Federal University, Svobodnuy Av. 79, Krasnoyarsk, Russian Federation
Laboratory of Anatomy, Histology, Embryology, School of Medicine, University of Crete, Heraklion, Greece
Institute of Physics, Russian Academy of Science, Siberian Division, Akademgorodok, 50, Build. 38, Krasnoyarsk, Russian Federation
Laboratory of Toxicology, Medical School, University of Crete, Heraklion, Greece
Доп.точки доступа:
Shishatskaya, E. I.; Шишацкая, Екатерина Игоревна; Nikitovic, D.; Shabanov, A. V.; Шабанов, Александр Васильевич; Tzanakakis, G. N.; Tsatsakis, A. M.; Menzianova, N. G.
}
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8.
Properties of a
novel quaterpolymer P(3HB/4HB/3HV/3HHx) / T. G. Volova [et al.]> // Polymer. - 2016. -
Vol. 101
. - P. 67-74,
DOI
10.1016/j.polymer.2016.08.048 . - ISSN 0032-3861
Кл.слова (ненормированные):
Physicochemical and mechanical properties
--
Polyhydroxyalkanoates
--
Quaterpolymers
--
Biocompatibility
--
Cell culture
--
Chain length
--
Chains
--
Decomposition
--
3-Hydroxyhexanoate
--
Degrees of crystallinity
--
Elongation at break
--
Physico-chemical and mechanical properties
--
Polyhydroxyalkanoates
--
Quaterpolymers
--
Short chain lengths
--
Thermal decomposition temperature
--
Film preparation
Аннотация:
Cupriavidus eutrophus В10646 was used to synthesize a series of polyhydroxyalkanoate (PHA) quaterpolymers composed of the short-chain-length 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB), and 3-hydroxyvalerate (3HV) and the medium-chain-length 3-hydroxyhexanoate (3HHx). The molar fraction of 3HB in the quaterpolymers varied between 63.5 and 93.1 mol.%, 3HV – between 1.1 and 24.6 mol.%, 4HB – between 2.4 and 15.6 mol.%, and 3HHx – between 0.4 and 4.8 mol.%. The properties of PHA quaterpolymers were significantly different from those of the P(3HB) homopolymer: they had much lower degrees of crystallinity (up to 30–45%), and lower melting points and thermal decomposition temperatures, with the interval between these temperatures remaining practically unchanged. Films prepared from PHA quaterpolymers were rougher and more porous than P(3HB) films; they showed higher values of elongation at break (up to 6–113%), i.e. were more elastic. Films prepared from PHA quaterpolymers were biocompatible and had no toxic effect on mouse fibroblast NIH 3T3 cells. © 2016 Elsevier Ltd
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Держатели документа:
Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, Russian Federation
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
L.V. Kirensky Institute of Physics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Volova, T. G.; Волова, Татьяна Григорьевна; Vinogradova, O. N.; Zhila, N. O.; Жила, Наталья Олеговна; Peterson, I. V.; Петерсон, Иван Викторович; Kiselev, E. G.; Киселев, Евгений Геннадьевич; Vasiliev, A. D.; Васильев, Александр Дмитриевич; Sukovatiy, A. G.; Суковатый, Алексей Григорьевич; Shishatskaya, E. I.; Шишацкая, Екатерина Игоревна
}
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9.
Влияние биоматериалов медицинского
назначения на структурно-функциональные особенности моноцитов / Н. Г. Мензянова [и др.]> // Журн. СФУ. Сер. "Биология". - 2016. -
Т. 9
,
№ 1
. - С. 33-42,
DOI
10.17516/1997-1389-2015-9-1-33-42 . - ISSN 1997-1389
Перевод заглавия:
Effect of Biomaterials of Medical Purpose on the Structural and Functional Features of Monocytes
Кл.слова (ненормированные):
biomaterials for medical uses
--
Polyhydroxyalkanoates
--
monocytes
--
nanodiamonds
--
fullerenes
--
биоматериалы медицинского назначения
--
моноциты
--
наноалмазы
--
фуллерены
--
полигидроксиалканоаты
Аннотация:
Изучены морфофункциональные особенности моноцитов в условиях кратковременного культивирования in vitro на носителях из биополимера полигидроксиалканоата (ПГА) в присутствии наноалмазов (НА) и фуллеренов (Ф). Показано, что НА и Ф в исследованных концентрациях не влияли на жизнеспособность моноцитов в кратковременной культуре. Соотношение морфологических классов моноцитов в присутствии НА и Ф существенно отличалось от контрольного варианта культивирования. Влияние НА и Ф характеризовалось дозовой зависимостью. Воздействие наночастиц на культуру моноцитов значительно изменялось при формировании белковой короны на поверхности НА и Ф, что приводило к возникновению новых количественных соотношений между морфологическими классами моноцитов в культуре in vitro. Полученные результаты позволяют рассматривать кратковременную культуру моноцитов in vitro как адекватную модельную систему для оценки влияния новых материалов на процессы дифференцировки субпопуляций моноцитов.
The morphological and functional features of the monocytes in a short-term in vitro cultivation on substrates of biopolymer polyhydroxyalkanoate (PHA) in the presence of nanodiamonds (ND) and fullerene (F) were studied. It has been shown that ND and F in the studied concentrations did not affect the viability of monocytes in the short-term culture. The ratio of morphological classes in the presence of ND and F signi?cantly different from the control group of cultivation. Effects of NA and F were characterized by dose-dependent. The formation of a protein corona on the surface of NA and F led to signi?cant changes in the effects of nanoparticles and the formation of new quantitative relationships between morphological classes of monocytes in culture in vitro. The results allow to consider a short-term culture of monocytes in vitro as a model system for adequate assessment of the impact of new materials on the processes of differentiation of monocyte subpopulations.
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Держатели документа:
Институт физики им. Л.В. Киренского СО РАН
Сибирский федеральный университет
Доп.точки доступа:
Мензянова, Н. Г.; Menzyanova N. G.; Николаева, Е. Д.; Nikolaeva E. D.; Винокурова, Д. В.; Vinokurova Daria V.; Шабанов, Александр Васильевич; Shabanov, A. V.; Шершнева, А. М.; Shershneva Anna M.; Шишацкая, Екатерина Игоревна; Shishatskaya E. I.; Сухова, К. В.; Sukhova Kristina V.
}
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10.
Физико-химические свойства многокомпонентных
полигидроксиалканоатов: новые аспекты / Т. Г. Волова [и др.]> // Высокомолекулярные соединения. Серия А. - 2017. -
Т. 59
,
№ 1
. - С. 76-85,
DOI
10.7868/S2308112017010163 . - ISSN 2308-1120
Перевод заглавия:
Physicochemical properties of multicomponent
polyhydroxyalkanoates
: novel aspects
Аннотация:
Исследованы физико-химические свойства (степень кристалличности, температурные и молекулярно-массовые характеристики) ряда полигидроксиалканоатов различного химического состава, синтезированные на комплексном углеродном субстрате бактериями Cupriavidus eutrophus В10646. Двух-, трех- и четырехкомпонентные сополимерные образцы различались набором и соотношением мономеров с различной длиной С-цепи: 3-гидроксибутирата (3ГБ), 4-гидроксибутирата (4ГБ), 3-гидроксивалерата (3ГВ), 3-гидроксигексаноата (3ГГ), 3-гидрокси-4-метилвалерата (3Г4МВ), диэтиленгликоля (ДЭГ). Показана вариабельность величин средневесовой молекулярной массы Мw и полидисперсности в широких пределах без связи с составом сополимерных полигидроксиалканоатов и сохранение термостабильности в интервале между температурой плавления и температурой термической деградации от 100 до 120–140°С. Состав и соотношение мономеров оказывали наиболее выраженное влияние на степень кристалличности полигидроксиалканоатов. Впервые показаны значительные отличия степени кристалличности у трех- и четырехкомпонентных полигидроксиалканоатов. Величина степени кристалличности у сополимеров П(3ГБ/3ГВ/4ГБ) составила 9–22%; у сополимеров П(3ГБ/3ГВ/3ГГ) и П(3ГБ/3ГВ/3Г4МВ) от 41 до 63%, что близко к величине степени кристалличности диблок-сополимеров П(3ГБ)/ДЭГ 56–69%. Четырехкомпонентные сополимеры П(3ГБ/3ГВ/4ГБ/3ГГ) имели значения степени кристалличности на уровне 30–41%. Зарегистрирована гетерогенность по величине Мw у сополимеров П(3ГБ)/ДЭГ и присутствие в полимере неравномерных по величине фракций: высокомолекулярного (Мw от 2700 до 4900 кДа) и низкомолекулярного полимера (Мw = 46–167 кДа). Для П(3ГБ)/ДЭГ и П(3ГБ/3ГВ/3Г4МВ) выявлено появление двух пиков в области плавления с разрывом между пиками от 4 до 20°С. Показано, что все типы сополимерных образцов, независимо от соотношения мономеров, имели в разной степени выраженное повышение значений удлинения при разрыве на фоне понижения напряжения при разрыве и модуля Юнга. В целом, многокомпонентные полигидроксиалканоаты значительно различаются свойствами.
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Держатели документа:
Институт физики им. Л.В. Киренского СО РАН
Доп.точки доступа:
Волова, Татьяна Григорьевна; Volova, T. G.; Виноградова, О. Н.; Жила, Наталья Олеговна; Zhila, N. O.; Киселев, Евгений Геннадьевич; Kiselev, E. G.; Петерсон, Иван Викторович; Peterson, I. V.; Васильев, Александр Дмитриевич; Vasiliev, A. D.; Суковатый, Алексей Григорьевич; Sukovatyǐ, A. G.; Шишацкая, Екатерина Игоревна; Shishatskaya, E. I.
}
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11.
Morphological aspects of
monocyte/macrophage polarization on biopolymer scaffolds in atherosclerosis patients / Menzyanova [et al.]> // J. Biotechnol. Biomater. - 2018. -
Vol. 8
,
Is. 3
. - Ст. 1000284,
DOI
10.4172/2155-952X.1000284. - Cited References: 67. - The study was supported by the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505). . - ISSN 2155-952X
Перевод заглавия:
Морфологические аспекты поляризации моноцитов / макрофагов на биополимерных каркасах у пациентов с атеросклерозом
Кл.слова (ненормированные):
Monocytes
--
Macrophages
--
Cell morphology
--
Polyhydroxyalkanoates
--
PHA
--
Copolymers
--
Atherosclerosis
--
Intravascular stenting
Аннотация:
The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured substrates, made from polyhydroxyalcanoates (PHAs) of various monomer compositions, prepared by casting polymer solutions on a defatted glass and followed by the solvent evaporation. Its surface relief, being further in direct contact with human cells in vitro was analyzed by AFM and SEM scanning. It was shown, that the features of micro/nano relief depend on the monomeric composition of polymer substrates. Monocytes (MNs) of patients with atherosclerosis and cardiac ischemia undergoing stenting and conventional anti-atherosclerotic therapy were harvested prior to and after stenting. After isolation MNs were cultured and transformed into MPh in direct contact with biopolymer culture substrates with different monomer composition and Nano-reliefs in comparison with culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described and their ratios for five samples of polymers were counted as an intermediate result in the development of “smart” material for blood vessel recovery. The results obtained allow us to assume that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates but also on the initial state of MNs in vivo and the general response of patients.
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Держатели документа:
Siberian Federal University, Svobodny, Krasnoyarsk, Russia
L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russia
Federal Research Centre Krasnoyarsk Scientific Centre of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russia
Federal Centres for Cardiovascular Surgery, Krasnoyarsk, Russia
Доп.точки доступа:
Menzyanova, Natalia G.; Pyatina, Svetlana V.; Nikolaeva, Elena D.; Shabanov, A. V.; Шабанов, Александр Васильевич; Nemtsev, I. V.; Stolyarov, D. P.; Dryganov, Dmitry B.; Sakhnov, Eugene V.; Vinokurova, Daria A.; Shishatskaya, Ekaterina I.
}
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12.
Biosynthesis and properties
of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646 / T. Volova [et al.]> // J. Chem. Technol. Biotechnol. - 2019. -
Vol. 94
,
Is. 1
. - P. 195-203,
DOI
10.1002/jctb.5763. - Cited References: 32. - This study was supported financially by Project ‘Agropreparations of the new generation: a strategy of construction and realization’ (Agreement No 074‐02‐2018‐328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, ‘On measures designed to attract leading scientists to the Russian institutions of higher learning’. . - ISSN 0268-2575
Кл.слова (ненормированные):
polyhydroxyalkanoates
--
3-hydroxy-4-methylvalerate
--
synthesis
--
physicochemical properties
Аннотация:
BACKGROUND:
Polyhydroxyalkanoates
(PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L−1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years.
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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
L.V. Kirenskii Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
International and Inter University Centre of Nanoscience and Nanotechnology, Mahatam Gandhi University, Kottyam, India
Доп.точки доступа:
Volova, T.; Menshikova, O.; Zhila, N.; Vasiliev, A. D.; Васильев, Александр Дмитриевич; Kiselev, E.; Peterson, I.; Shishatskaya, E.; Thomas, S.
}
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13.
Screening of biopolymeric
materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients / N. G. Menzyanova [et al.]> // Toxicol. Rep. - 2019. -
Vol. 6
. - P. 74-90,
DOI
10.1016/j.toxrep.2018.11.009. - Cited References: 85. - The study was supported by the Russian Science Foundation , Project No 17-15-01352 . . - ISSN 2214-7500
Перевод заглавия:
Скрининг биополимерных материалов на токсичность для сердечно-сосудистой хирургии - оценка рельефа их поверхности с оценкой морфологических аспектов поляризации моноцитов / макрофагов у больных атеросклерозом
Кл.слова (ненормированные):
Monocytes
--
Macrophages
--
Cell morphology
--
Polyhydroxyalkanoates
--
Atherosclerosis
--
Intravascular stenting
Аннотация:
The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients.
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Держатели документа:
Siberian Federal University, 79, Svobodny av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Center for Cardiovascular Surgery, 45 Karaulnaya, Krasnoyarsk, 660020, Russian Federation
Доп.точки доступа:
Menzyanova, N. G.; Pyatina, S. А.; Nikolaeva, E. D.; Shabanov, A. V.; Шабанов, Александр Васильевич; Nemtsev, I. V.; Stolyarov, D. P.; Dryganov, D. B.; Sakhnov, E. V.; Shishatskaya, E. I.
}
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14.
Properties of degradable
polyhydroxyalkanoates
(PHAs) synthesized by a new strain, Cupriavidus necator IBP/SFU-1, from various carbon sources / N. O. Zhila, K. Yu. Sapozhnikova, E. G. Kiselev [et al.]> // Polymers. - 2021. -
Vol. 13
,
Is. 18
. - Ст. 3142,
DOI
10.3390/polym13183142. - Cited References: 78. - This work was financially supported by Project “Agropreparations of the new generation: a strategy of construction and realization” (Agreement No 075-15-2021-626) in accordance with Resolution No 220 of the Government of the Russian Federation of 9 April 2010, “On measures designed to attract leading scientists to the Russian institutions of higher learning” (strain isolation, polymer synthesis and investigation), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (study of film properties) . - ISSN 2073-4360
Кл.слова (ненормированные):
Cupriavidus necator IBP/SFU-1
--
cell growth and PHA synthesis
--
various carbon sources
--
PHA composition and properties
--
polymer films
Аннотация:
The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.
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Держатели документа:
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center SB RAS”, L.V. Kirensky Institute of Physics SB RAS, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center, “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Zhila, N. O.; Sapozhnikova, K. Yu.; Kiselev, E. G.; Vasiliev, A. D.; Васильев, Александр Дмитриевич; Nemtsev, I. V.; Немцев, Иван Васильевич; Shishatskaya, E. I.; Volova, T. G.
}
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15.
Chemical modification of
films from biosynthetic poly-3-hydroxybutyrate aimed to improvement of their surface properties / A. N. Boyandin, A. A. Sukhanova, E. D. Nikolaeva, I. V. Nemtsev> // Macromolecular Symposia. - 2021. -
Vol. 395
: 4th International Conference on Progress on Polymers and Composites (NOV 26-28, 2020, Electr. Network),
Is. 1
. - Ст. 2000281,
DOI
10.1002/masy.202000281. - Cited References: 11. - This work was carried out by the team of the scientific laboratory "Smart Materials and Structures" within the state assignment of the Ministry of Science and Higher Education of the Russian Federation for the implementation of the project "Development of multifunctional smart materials and structures based on modified polymer composite materials capable to function in extreme conditions" (No. FEFE-2020-0015). The surface of the samples was investigated using a scanning electron microscope Hitachi TM3000 in the Krasnoyarsk Regional Center of Research Equipment of Federal Research Center "Krasnoyarsk Science Center SB RAS" . - ISSN 1022-1360. - ISSN 1521-3900
Перевод заглавия:
Химическая модификация плёнок из биосинтетического поли-3-гидроксибутирата, направленная на улучшение их поверхностных свойств
РУБ
Polymer Science
Кл.слова (ненормированные):
biocompatibility
--
polyhydroxyalkanoates
--
polyhydroxybutyrate
--
polymer modification
Аннотация:
Films from biodegradable poly-3-hydroxybutyrate are treated with chemical reagents to improve their hydrophilicity and biocompatibility. Two approaches are tested: a single treatment with alkali, acids, oxidizing or reducing agents, and a step-by step treatment of the alkali pre-activated surface of polymer films with bromine water and amino-compounds (ammonia or triethylamine). The maximal level of hydrophilicity (the lowest water contact angle and the highest polar component of the surface free energy) is registered after a single treatment with NaOH and after the step-by-step treatment. These samples also showed the best adhesion of mouse fibroblasts of NIH 3T3 line on the film surface. So, the proposed methods can be used to enhance hydropilicity and biocompatibility of biopolymer surface.
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Держатели документа:
Reshetnev Siberian State Univ Sci & Technol, 31 Krasnoyarsky Rabochy Av, Krasnoyarsk 660037, Russia.
Russian Acad Sci, Siberian Branch, Fed Res Ctr, Inst Biophys,Krasnoyarsk Sci Ctr, 50,Build 50, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Int Sci Ctr Studying Extreme States Organism, Fed Res Ctr,Krasnoyarsk Sci Ctr, 50,Build 12-2, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Fed Res Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.
Доп.точки доступа:
Boyandin, A. N.; Sukhanova, Anna Alekseevna; Nikolaeva, Elena Dmitrievna; Nemtsev, I. V.; Немцев, Иван Васильевич; Ministry of Science and Higher Education of the Russian Federation [FEFE-2020-0015]; International Conference on Progress on Polymers and Composites(4th ; 26-28 Nov. 2020)
}
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16.
Киселев, Евгений Геннадьевич
.
Синтез и характеристики многокомпонентных ПГА / Е. Г. Киселев, А. Д. Васильев, Т. Г. Волова> // Журн. СФУ. Биология. - 2021. -
Т. 14
,
№ 1
. - С. 97-113 ; J. Sib. Fed. Univ. Biol.,
DOI
10.17516/1997-1389-0325. - Библиогр.: 32. - Работа выполнена по гранту No 19-43-240012 «Биологические и физические принципы получения биоматериалов новой генерации» при поддержке РФФИ и ККФН и госзаданию Министерства науки и высшего образования РФ No FSRZ-2020-0006. В работе использовано оборудование Центра коллективного пользования приборами ФИЦ «КНЦ СО РАН» . - ISSN 1997-1389. - ISSN 2313-5530
Перевод заглавия:
Synthesis and Characterization of Multicomponent PHAs
Кл.слова (ненормированные):
полигидроксиалканоаты (ПГА)
--
биосинтез
--
субстраты-предшественники
--
сополимеры
--
физико-химические свойства
--
polyhydroxyalkanoates
(PHAs)
--
biosynthesis
--
precursor substrates
--
copolymers
--
physicochemical properties
Аннотация:
В культуре бактерий Cupriavidus necator B10646 в режиме синтеза резервных полигидроксиалканоатов при включении в состав ростовой среды, помимо основного субстрата, глюкозы, субстратов-предшественников различных мономеров монокарбоновых кислот – солей валериановой и гексановой кислот, пропионата, γ-бутиролактона, синтезировано семейство трех- и четырехкомпонентных полимеров с различным набором и соотношением мономеров и исследованы физико-химические свойства. Трехкомпонентные образцы были образованы мономерами 3-гидроксибутирата (3ГБ), 3-гидроксивалерата (3ГВ), 4-гидроксибутирата (4ГБ) или 3-гидроксигексаноата (3ГГ) и имели следующие составы: П(3ГБ/3ГВ/4ГБ) и П(3ГБ/3ГВ/4ГГ); четырехкомпонентные сополимеры имели состав П(3ГБ/3ГВ/4ГБ/3ГГ). Все сополимерные образцы, независимо от состава и соотношения мономеров, обладали сниженными значениями молекулярной массы и повышенными значениями полидисперсности по сравнению с высококристалличным гомополимером 3-гидроксибутирата, но сохраняли свойства термостабильности, имея разрыв между температурой плавления и термической деградации не менее 100-110 °С. Включение в С-цепь 3ГБ мономеров 3ГВ, 4ГБ и 3ГГ вызывало значительные изменения соотношения кристаллической и аморфной фаз и снижение степени кристалличности (Сх), зависящие от типа мономеров и их содержания в сополимере. Максимальное снижение Сх (до 9-17 %) зарегистрировано у трехкомпонентных образцов П(3ГБ/3ГВ/4ГБ), у четырехкомпонентных сополимеров П(3ГБ/3ГВ/4ГБ/3ГГ) – до 30-36 %. Доказана возможность синтеза полимеров разного, в том числе нового, состава, существенно различающихся базовыми свойствами.
Cupriavidus necator B10646 bacterial cells were cultivated in the mode of synthesis of the reserve
polyhydroxyalkanoates
(PHAs) in the growth medium that contained, in addition to glucose as the main substrate, precursor substrates of the monomers of various monocarboxylic acids – salts of valeric and hexanoic acids, propionate, and γ-butyrolactone. PHA terpolymers and quaterpolymers with different compositions and proportions of monomers were synthesized, and their physicochemical properties were studied. The terpolymers were composed of monomers of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), or 3-hydroxyhexanoate (3HHx) and had the following compositions: P(3HB/3HB/4HHx) and P(3HB/3HV/4HHx). The quaterpolymers had the following composition: P(3HB/3HV/4HB/3HHx). All copolymer samples, regardless of the composition and proportions of monomers, had lower molecular weights and higher polydispersity values compared to the highly crystalline 3-hydroxybutyrate homopolymer, but retained thermal stability properties, with a difference between the melting point and thermal degradation of at least 100-110 °С. The inclusion of 3HV, 4HB, and 3HHx monomers in the C-chain of 3HB caused changes in the crystalline to amorphous phase ratio and a significant decrease in the degree of crystallinity (Cx), which depended on the type of monomers and their contents in the copolymer. The maximum decrease in Cx (9-17 %) was detected in the P(3HB/3HV/4HB) terpolymer and the P(3HB/3HV/4HB/3HHx) quaterpolymer (30-36 %). The study confirms that there is the possibility of synthesizing polymers with various compositions, including new ones, which differ significantly in their basic properties.
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Держатели документа:
Институт биофизики СО РАН ФИЦ «Красноярский научный центр СО РАН», Российская Федерация, Красноярск
Сибирский федеральный университет, Российская Федерация, Красноярск
Институт физики им. Л.В. Киренского ФИЦ «Красноярский научный центр СО РАН», Российская Федерация, Красноярск
Доп.точки доступа:
Васильев, Александр Дмитриевич; Vasiliev, A. D.; Волова, Т. Г.
}
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17.
Properties of degradable
polyhydroxyalkanoates
with different monomer compositions / T. Volova, E. Kiselev, I. Nemtsev [et al.]> // Int. J. Biol. Macromol. - 2021. -
Vol. 182
. - P. 98-114,
DOI
10.1016/j.ijbiomac.2021.04.008. - Cited References: 109. - The part of the reported study on polymer synthesis and examination was funded by RFBR and KKRF [Grant No. 19-43-240012 “Biological and physical principles of production of new generation biomaterials”]. The work on production and investigation of polymer films was carried out as part of the State Assignment of the Ministry of Education and Science of the Russian Federation [Grant No. FSRZ-2020-0006] . - ISSN 0141-8130
Кл.слова (ненормированные):
Physicochemical properties
--
Chemical composition
--
Physicochemical properties
--
Films
--
Microstructure
--
Surface properties
Аннотация:
Purpose: To synthesize and investigate
polyhydroxyalkanoates
(PHAs) with different monomer composition and percentages and polymer films prepared from them. Results: Various PHAs: homopolymer poly-3-hydroxybutyrate P(3HB) and 2-, 3-, and 4-component copolymers comprising various combinations of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), and 3-hydroxyhexanoate (3HHx) monomers were synthesized under specialized conditions. Relationships were found between the monomer composition of PHAs and their molecular-weight and thermal properties and degree of crystallinity. All copolymers had decreased weight average molecular weights, Mw (to 390–600 kDa), and increased values of polydispersity (3.2–4.6) compared to the P(3HB). PHA copolymers showed different thermal behavior: an insignificant decrease in Tmelt and the presence of the second peak in the melting region and changes in parameters of crystallization and glass transition. At the same time, they retained thermostability, and the difference between Tmelt and Tdegr was at least 100–120 °C. Incorporation of 4HB, 3HV, and 3HHx monomer units into the 3-hydroxybutyrate chain caused changes in the amorphous to crystalline ratio and decreased the degree of crystallinity (Cx) to 20–40%. According to the degree to which the monomers reduced crystallinity, they were ranked as follows: 4HB – 3HHx – 3HV. A unique set of films was produced; their surface properties and physical/mechanical properties were studied as dependent on PHA composition; monomers other than 3-hydroxybutyrate were found to enhance hydrophilicity, surface development, and elasticity of polymer films. Conclusion: An innovative set of PHA copolymers was synthesized and solution-cast films were prepared from them; the copolymers and films were investigated as dependent on polymer chemical composition. Results obtained in the present study contribute to the solution of a critical issue of producing degradable polymer materials.
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Держатели документа:
Siberian Federal University, 79 Svobodnyi av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/12 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Volova, T.; Kiselev, E.; Nemtsev, I. V.; Немцев, Иван Васильевич; Lukyanenko, A. V.; Лукьяненко, Анна Витальевна; Sukovatyi, A.; Kuzmin, A.; Ryltseva, G.; Shishatskaya, E.
}
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18.
Production and properties
of microbial
polyhydroxyalkanoates
synthesized from hydrolysates of Jerusalem artichoke tubers and vegetative biomass / T. G. Volova, E. G. Kiselev, A. V. Demidenko [et al.]> // Polymers. - 2022. -
Vol. 14
,
Is. 1
. - Ст. 132,
DOI
10.3390/polym14010132. - Cited References: 93. - This study was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No. 075-15-2021-626) in accordance with Resolution No. 220 of the Government of the Russian Federation of 9 April 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning" (polymer synthesis, properties), and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (films production, surface properties) . - ISSN 2073-4360
РУБ
Polymer Science
Рубрики:
GLUCOSE-UTILIZING STRAIN
RALSTONIA-EUTROPHA
ASPERGILLUS-NIGER
ACID
Кл.слова (ненормированные):
Jerusalem artichoke hydrolysates
--
PHA synthesis
--
productivity
--
polyhydroxyalkanoates
Аннотация:
One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains—C. necator B-10646 and R. eutropha B 5786 and B 8562—were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 °C followed by acid hydrolysis at 60 °C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100–120 °C difference between the Tmelt and Tdegr, prevailing of the crystalline phase over the amorphous one (Cx between 69 and 75%), and variations in weight average molecular weight (409–480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and ε-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology—finding widely available and inexpensive substrates.
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Держатели документа:
Siberian Fed Univ, Sch Fundamental Biol & Biotechnol, Basic Dept Biotechnol, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys, SB RAS, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, LV Kirensky Phys Inst, Fed Res Ctr, SB RAS, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, Krasnoyarsk 660036, Russia.
Доп.точки доступа:
Volova, Tatiana G.; Kiselev, Evgeniy G.; Demidenko, Alexey V.; Zhila, Natalia O.; Nemtsev, I. V.; Немцев, Иван Васильевич; Lukyanenko, A. V.; Лукьяненко, Анна Витальевна; Project "Agropreparations of the new generation: a strategy of construction and realization" [075-15-2021-626]; State Assignment of the Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
}
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19.
Biodegradable
polyhydroxyalkanoates
formed
by 3- and 4-hydroxybutyrate monomers to produce nanomembranes suitable for drug delivery and cell culture / T. G. Volova, A. V. Demidenko, A. V. Murueva [et al.]> // Technologies. - 2023. -
Vol. 11
,
Is. 4
. - Ст. 106,
DOI
10.3390/technologies11040106. - Cited References: 116. - his research was funded by State Assignment of the Ministry of Science and Higher Education of the Russian Federation (project No. 0287-2021-0025) . - ISSN 2227-7080
Кл.слова (ненормированные):
degradable
polyhydroxyalkanoates
--
copolymers P(3HB-co-4HB)
--
electrostatic molding
--
membranes
--
microstructure and properties
--
drugs
--
release kinetics
--
antibacterial activity
--
fibroblast proliferation
Аннотация:
Biodegradable
polyhydroxyalkanoates
, biopolymers of microbiological origin, formed by 3- and 4-hydroxybutyrate monomers P(3HB-co-4HB), were used to obtain nanomembranes loaded with drugs as cell carriers by electrospinning. Resorbable non-woven membranes from P(3HB-co-4HB) loaded with ceftazidime, doripinem, and actovegin have been obtained. The loading of membranes with drugs differently affected the size of fibers and the structure of membranes, and in all cases increased the hydrophilicity of the surface. The release of drugs in vitro was gradual, which corresponded to the Higuchi and Korsmeyer-Peppas models. Antibiotic-loaded membranes showed antibacterial activity against S. aureus and E. coli, in which growth inhibition zones were 41.7 ± 1.1 and 38.6 ± 1.7 mm for ceftazidime and doripinem, respectively. The study of the biological activity of membranes in the NIH 3T3 mouse fibroblast culture based on the results of DAPI and FITC staining of cells, as well as the MTT test, did not reveal a negative effect despite the presence of antibiotics in them. Samples containing actovegin exhibit a stimulating effect on fibroblasts. Biodegradable
polyhydroxyalkanoates
formed by 3-hydroxybutyrate and 4-hydroxybutyrate monomers provide electrospinning non-woven membranes suitable for long-term delivery of drugs and cultivation of eukaryotic cells, and are promising for the treatment of wound defects complicated by infection.
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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, Russia
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi av., 660041 Krasnoyarsk, Russia
Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50 Akademgorodok, 660036 Krasnoyarsk, Russia
L.V. Kirensky Institute of Physics, 50/12 Akademgorodok, 660036 Krasnoyarsk, Russia
Chemistry Engineering Centre, ITMO University, Kronverkskiy Prospekt, 49A, 197101 Saint Petersburg, Russia
Доп.точки доступа:
Volova, Tatiana G.; Demidenko, Aleksey V.; Murueva, Anastasiya V.; Dudaev, Alexey E.; Nemtsev, I. V.; Немцев, Иван Васильевич; Shishatskaya, Ekaterina I.
}
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20.
Biodegradable
polyhydroxyalkanoates
with
a different set of valerate monomers: Chemical structure and physicochemical properties / T. G. Volova, N. O. Zhila, E. G. Kiselev [et al.]> // Int. J. Mol. Sci. - 2023. -
Vol. 24
,
Is. 18
. - Ст. 14082,
DOI
10.3390/ijms241814082. - Cited References: 99. - The study was funded by the Russian Science Foundation (project no. 23-64-10007) . - ISSN 1661-6596. - ISSN 1422-0067
Кл.слова (ненормированные):
copolymers
--
P(3HB-co-3HV-co-4HV)
--
P(3HB-co-3HV-co-3H4MV)
--
physicochemical properties
--
thermal behavior
--
isothermal crystallization
--
spherulites formation rate
--
morphology
Аннотация:
The properties, features of thermal behavior and crystallization of copolymers containing various types of valerate monomers were studied depending on the set and ratio of monomers. We synthesized and studied the properties of three-component copolymers containing unusual monomers 4-hydroxyvalerate (4HV) and 3-hydroxy-4-methylvalerate (3H4MV), in addition to the usual 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) monomers. The results showed that P(3HB-co-3HV-co-4HV) and P(3HB-co-3HV-co-3H4MV) terpolymers tended to increase thermal stability, especially for methylated samples, including an increase in the gap between melting point (Tmelt) and thermal degradation temperature (Tdegr), an increase in the melting point and glass transition temperature, as well as a lower degree of crystallinity (40–46%) compared with P(3HB-co-3HV) (58–66%). The copolymer crystallization kinetics depended on the set and ratio of monomers. For terpolymers during exothermic crystallization, higher rates of spherulite formation (Gmax) were registered, reaching, depending on the ratio of monomers, 1.6–2.0 µm/min, which was several times higher than the Gmax index (0.52 µm/min) for the P(3HB-co-3HV) copolymer. The revealed differences in the thermal properties and crystallization kinetics of terpolymers indicate that they are promising polymers for processing into high quality products from melts.
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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, Russia
Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia
L.V. Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/38 Akademgorodok, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Volova, Tatiana G.; Zhila, Natalia O.; Kiselev, Evgeniy G.; Sukovatyi, Aleksey G.; Lukyanenko, A. V.; Лукьяненко, Анна Витальевна; Shishatskaya, Ekaterina I.
}
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