Библиотека института физики им. Л.В. Киренского СО РАН

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Каталог книг и брошюр библиотеки ИФ СО РАН

Каталог журналов библиотеки ИФ СО РАН

Труды сотрудников ИФ СО РАН

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E. I., Nikitovic D., Shabanov A. V., Tzanakakis G. N., Tsatsakis A. M., Menzianova N. G.
Заглавие : Short-term culture of monocytes as an in vitro evaluation system for bionanomaterials designated for medical use
Место публикации : Food Chem. Toxicol.: Elsevier, 2016. - Vol. 96. - P.302-308. - ISSN 02786915 (ISSN), DOI 10.1016/j.fct.2016.08.025
Примечания : Cited References: 46
Предметные рубрики: MAGNETIC NANOPARTICLES
CELLULAR REDUCTION
PROTEIN CORONA
CELLS
MECHANOTRANSDUCTION
NANOMATERIALS
LOCALIZATION
BIOMATERIALS
CYTOSKELETON
ACTIVATION
Ключевые слова (''Своб.индексиров.''): biocompatible biopolymers--polyhydroxyalkanoates--nanomaterials--nanodiamonds--fullerenes--bio-nanomaterials--monocytes
Аннотация: We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the viability/metabolic activity of cells. The scanning electron microscopy assay can detect fine changes in cell morphology. In the present study BNM have been shown to affect; in a size, chemical composition and morphological characteristics-dependent manner, the ability of monocytes to reduce MTT as well as their morphology. Moreover, the specific effects of ND and F on MTT reduction and cell morphology were exhibited in a dose-dependent manner and sensitive to the formation of surface protein corona. Our results suggest that short-term culture of monocytes is a sensitive model system for assessing the biological effects of BMPs in vitro. © 2016 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Menzyanova N. G., Pyatina S. А., Nikolaeva E. D., Shabanov A. V., Nemtsev I. V., Stolyarov D. P., Dryganov D. B., Sakhnov E. V., Shishatskaya E. I.
Заглавие : Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients
Место публикации : Toxicol. Rep. - 2019. - Vol. 6. - P.74-90. - ISSN 22147500 (ISSN), DOI 10.1016/j.toxrep.2018.11.009
Примечания : Cited References: 85. - The study was supported by the Russian Science Foundation , Project No 17-15-01352 .
Ключевые слова (''Своб.индексиров.''): monocytes--macrophages--cell morphology--polyhydroxyalkanoates--atherosclerosis--intravascular stenting
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stolyar S. V., Kolenchukova, Oksana A., Boldyreva, Anna V., Kudryasheva, Nadezda S., Gerasimova Yu. V., Krasikov A. A., Yaroslavtsev R. N., Bayukov O. A., Ladygina, Valentina P., Birukova, Elena A.
Заглавие : Biogenic ferrihydrite nanoparticles: Synthesis, properties in vitro and in vivo testing and the concentration effect
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Government of the Krasnoyarsk Territory; Regional Science Foundation [20-416-242907]
Место публикации : Biomedicines. - 2021. - Vol. 9, Is. 3. - Ст.323. - ISSN 2227-9059(eISSN), DOI 10.3390/biomedicines9030323
Примечания : Cited References: 52. - This research was funded by the Russian Foundation for Basic Research, the Government of the Krasnoyarsk Territory and the Regional Science Foundation, grant number 20-416-242907
Аннотация: Biogenic ferrihydrite nanoparticles were synthesized as a result of the cultivation of Klebsiella oxytoca microorganisms. The distribution of nanoparticles in the body of laboratory animals and the physical properties of the nanoparticles were studied. The synthesized ferrihydrite nanoparticles are superparamagnetic at room temperature, and the characteristic blocking temperature is 23-25 K. The uncompensated moment of ferrihydrite particles was determined to be approximately 200 Bohr magnetons. In vitro testing of different concentrations of ferrihydrite nanoparticles for the functional activity of neutrophilic granulocytes by the chemiluminescence method showed an increase in the release of primary oxygen radicals by blood phagocytes when exposed to a minimum concentration and a decrease in secondary radicals when exposed to a maximum concentration. In vivo testing of ferrihydrite nanoparticles on Wister rats showed that a suspension of ferrihydrite nanoparticles has chronic toxicity, since it causes morphological changes in organs, mainly in the spleen, which are characterized by the accumulation of hemosiderin nanoparticles (stained blue according to Perls). Ferrihydrite can also directly or indirectly stimulate the proliferation and intracellular regeneration of hepatocytes. The partial detection of Perls-positive cells in the liver and kidneys can be explained by the rapid elimination from organs and the high dispersion of the nanomaterial. Thus, it is necessary to carry out studies of these processes at the systemic level, since the introduction of nanoparticles into the body is characterized by adaptive-proliferative processes, accompanied by the development of cell dystrophy and tension of the phagocytic system.
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