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Вид документа : Статья из журнала Шифр издания :
Автор(ы) : Bondar' V. S., Pozdnyakova I. O., Puzyr' A. P.
Заглавие : Applications of nanodiamonds for separation and purification of proteins
Место публикации : Phys. Solid State: AMER INST PHYSICS, 2004. - Vol. 46, Is. 4. - P758-760. - ISSN 1063-7834, DOI 10.1134/1.1711468 Примечания : Cited References: 11
Предметные рубрики: ESCHERICHIA-COLI OBELIN Аннотация: Recombinant apoobelin and recombinant luciferase are separated from bacterial cells of Escherichia coli with the use of detonation nanodiamonds. The application of nanodiamonds has a number of points in its favor, namely, (i) simplifies the procedures for purifying the proteins, (ii) decreases the time of their separation to 30-40 min, (iii) eliminates the necessity of using special chromatographic equipment, and (iv) makes it possible to prepare high-purity apoobelin and luciferase materials with protein yields of 35-45 and 45-60%, respectively. The possible mechanisms of interaction of protein molecules and nanodiamond particles are analyzed. (C) 2004 MAIK "Nauka / Interperiodica".
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