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Вид документа : Статья из журнала Шифр издания :
Автор(ы) : Krasitskaya V. V., Goncharova N. S., Biriukov V. V., Bashmakova E. E., Kabilov M. R., Baykov I. K., Sokolov A. Е., Frank L. A.
Заглавие : The Ca2+-regulated photoprotein obelin as a tool for SELEX monitoring and DNA aptamer affinity evaluation
Коллективы :
Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]
Место публикации : Photochem. Photobiol. - 2020. - Vol. 96, Is. 5. - P.1041-1046. - ISSN 0031-8655, DOI 10.1111/php.13274. - ISSN 1751-1097(eISSN) Примечания : Cited References: 25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531.
Предметные рубрики: CARDIAC TROPONIN-I BIOLUMINESCENCE IMMUNOASSAY APTASENSOR DIAGNOSIS Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.
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