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Вид документа : Статья из журнала
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Автор(ы) : Shishatskaya E. I., Nikitovic D., Shabanov A. V., Tzanakakis G. N., Tsatsakis A. M., Menzianova N. G.
Заглавие : Short-term culture of monocytes as an in vitro evaluation system for bionanomaterials designated for medical use
Место публикации : Food Chem. Toxicol.: Elsevier, 2016. - Vol. 96. - P.302-308. - ISSN 02786915 (ISSN), DOI 10.1016/j.fct.2016.08.025
Примечания : Cited References: 46
Предметные рубрики: MAGNETIC NANOPARTICLES
CELLULAR REDUCTION
PROTEIN CORONA
CELLS
MECHANOTRANSDUCTION
NANOMATERIALS
LOCALIZATION
BIOMATERIALS
CYTOSKELETON
ACTIVATION
Ключевые слова (''Своб.индексиров.''): biocompatible biopolymers--polyhydroxyalkanoates--nanomaterials--nanodiamonds--fullerenes--bio-nanomaterials--monocytes
Аннотация: We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the viability/metabolic activity of cells. The scanning electron microscopy assay can detect fine changes in cell morphology. In the present study BNM have been shown to affect; in a size, chemical composition and morphological characteristics-dependent manner, the ability of monocytes to reduce MTT as well as their morphology. Moreover, the specific effects of ND and F on MTT reduction and cell morphology were exhibited in a dose-dependent manner and sensitive to the formation of surface protein corona. Our results suggest that short-term culture of monocytes is a sensitive model system for assessing the biological effects of BMPs in vitro. © 2016 Elsevier Ltd
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