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Collagen conjugation to carboxyl-modified poly(3-hydroxybutyrate) microparticles: preparation, characterization and evaluation in vitro / A. V. Murueva, A. M. Shershneva, I. V. Nemtsev [et al.] // J. Polym. Res. - 2022. - Vol. 29, Is. 8. - Ст. 324, DOI 10.1007/s10965-022-03181-5. - Cited References: 45. - This work was supported by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020–0006 . - ISSN 1022-9760
Кл.слова (ненормированные):
Poly(3-hydroxybutyrate) -- Collagen -- Microparticles -- Surface conjugation -- Cytotoxicity
Аннотация: The objective of this study was to produce and characterize collagen conjugated poly(3-hydroxybutyrate) (P3HB) microparticles. The double emulsion solvent evaporation technique was used to fabricate biopolymer microparticles. Alkaline hydrolysis method was used for carboxylation of P3HB microparticles followed by collagen (type I) conjugation using 1-ethyl-3-(3-dime-thylaminopropyl) carbodiimide and N-hydroxysuccinimide cross linkers. The successful chemical modification of P3HB-MP surface was evidenced by XPS analysis and FTIR spectroscopy, confirming an increase in the number of carboxyl groups and covalent bonding of collagen on the surface of functionalized P3HB microparticles (Col-P3HB-MPs). The amount of collagen present on Col-P3HB-MPs microparticles was quantified by the Lowry method. The average diameter and zeta-potential of microparticles were found to be dependent upon the method of preparation. The absence of cytotoxicity of modified Col-P3HB-MPs to fibroblast NIH 3T3 cells in vitro was corroborated by results of MTT assay. Staining with the fluorescent dye showed enhanced adhesion of fibroblasts onto Col-P3HB-MPs compared to the unmodified P3HB-MPs.

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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences (IBP SB RAS), 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041, Russian Federation
Federal Research Center Krasnoyarsk Scientific Center of the Siberian Branch of the RAS (FRC KSC SB RAS), 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS, 50/38 Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Murueva, A. V.; Shershneva, A. M.; Nemtsev, I. V.; Немцев, Иван Васильевич; Shishatskaya, E. I.; Volova, T. G.
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