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    Влияние биоматериалов медицинского назначения на структурно-функциональные особенности моноцитов / Н. Г. Мензянова [и др.] // Журн. СФУ. Сер. "Биология". - 2016. - Т. 9, № 1. - С. 33-42, DOI 10.17516/1997-1389-2015-9-1-33-42 . - ISSN 1997-1389
   Перевод заглавия: Effect of Biomaterials of Medical Purpose on the Structural and Functional Features of Monocytes
Кл.слова (ненормированные):
biomaterials for medical uses -- Polyhydroxyalkanoates -- monocytes -- nanodiamonds -- fullerenes -- биоматериалы медицинского назначения -- моноциты -- наноалмазы -- фуллерены -- полигидроксиалканоаты
Аннотация: Изучены морфофункциональные особенности моноцитов в условиях кратковременного культивирования in vitro на носителях из биополимера полигидроксиалканоата (ПГА) в присутствии наноалмазов (НА) и фуллеренов (Ф). Показано, что НА и Ф в исследованных концентрациях не влияли на жизнеспособность моноцитов в кратковременной культуре. Соотношение морфологических классов моноцитов в присутствии НА и Ф существенно отличалось от контрольного варианта культивирования. Влияние НА и Ф характеризовалось дозовой зависимостью. Воздействие наночастиц на культуру моноцитов значительно изменялось при формировании белковой короны на поверхности НА и Ф, что приводило к возникновению новых количественных соотношений между морфологическими классами моноцитов в культуре in vitro. Полученные результаты позволяют рассматривать кратковременную культуру моноцитов in vitro как адекватную модельную систему для оценки влияния новых материалов на процессы дифференцировки субпопуляций моноцитов.
The morphological and functional features of the monocytes in a short-term in vitro cultivation on substrates of biopolymer polyhydroxyalkanoate (PHA) in the presence of nanodiamonds (ND) and fullerene (F) were studied. It has been shown that ND and F in the studied concentrations did not affect the viability of monocytes in the short-term culture. The ratio of morphological classes in the presence of ND and F signi?cantly different from the control group of cultivation. Effects of NA and F were characterized by dose-dependent. The formation of a protein corona on the surface of NA and F led to signi?cant changes in the effects of nanoparticles and the formation of new quantitative relationships between morphological classes of monocytes in culture in vitro. The results allow to consider a short-term culture of monocytes in vitro as a model system for adequate assessment of the impact of new materials on the processes of differentiation of monocyte subpopulations.

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Держатели документа:
Институт физики им. Л.В. Киренского СО РАН
Сибирский федеральный университет

Доп.точки доступа:
Мензянова, Н. Г.; Menzyanova N. G.; Николаева, Е. Д.; Nikolaeva E. D.; Винокурова, Д. В.; Vinokurova Daria V.; Шабанов, Александр Васильевич; Shabanov, A. V.; Шершнева, А. М.; Shershneva Anna M.; Шишацкая, Екатерина Игоревна; Shishatskaya E. I.; Сухова, К. В.; Sukhova Kristina V.
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Short-term culture of monocytes as an in vitro evaluation system for bionanomaterials designated for medical use / E. I. Shishatskaya [et al.] // Food Chem. Toxicol. - 2016. - Vol. 96. - P. 302-308, DOI 10.1016/j.fct.2016.08.025. - Cited References: 46 . - ISSN 0278-6915

РУБ Food Science & Technology + Toxicology
Рубрики:
MAGNETIC NANOPARTICLES
   CELLULAR REDUCTION
   PROTEIN CORONA
   CELLS
   MECHANOTRANSDUCTION
   NANOMATERIALS
   LOCALIZATION
   BIOMATERIALS
   CYTOSKELETON
   ACTIVATION
Кл.слова (ненормированные):
Biocompatible biopolymers -- Polyhydroxyalkanoates -- Nanomaterials -- Nanodiamonds -- Fullerenes -- Bio-nanomaterials -- Monocytes
Аннотация: We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the viability/metabolic activity of cells. The scanning electron microscopy assay can detect fine changes in cell morphology. In the present study BNM have been shown to affect; in a size, chemical composition and morphological characteristics-dependent manner, the ability of monocytes to reduce MTT as well as their morphology. Moreover, the specific effects of ND and F on MTT reduction and cell morphology were exhibited in a dose-dependent manner and sensitive to the formation of surface protein corona. Our results suggest that short-term culture of monocytes is a sensitive model system for assessing the biological effects of BMPs in vitro. © 2016 Elsevier Ltd

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Держатели документа:
Siberian Federal University, Svobodnuy Av. 79, Krasnoyarsk, Russian Federation
Laboratory of Anatomy, Histology, Embryology, School of Medicine, University of Crete, Heraklion, Greece
Institute of Physics, Russian Academy of Science, Siberian Division, Akademgorodok, 50, Build. 38, Krasnoyarsk, Russian Federation
Laboratory of Toxicology, Medical School, University of Crete, Heraklion, Greece

Доп.точки доступа:
Shishatskaya, E. I.; Шишацкая, Екатерина Игоревна; Nikitovic, D.; Shabanov, A. V.; Шабанов, Александр Васильевич; Tzanakakis, G. N.; Tsatsakis, A. M.; Menzianova, N. G.
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    Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients / N. G. Menzyanova [et al.] // Toxicol. Rep. - 2019. - Vol. 6. - P. 74-90, DOI 10.1016/j.toxrep.2018.11.009. - Cited References: 85. - The study was supported by the Russian Science Foundation , Project No 17-15-01352 . . - ISSN 2214-7500
   Перевод заглавия: Скрининг биополимерных материалов на токсичность для сердечно-сосудистой хирургии - оценка рельефа их поверхности с оценкой морфологических аспектов поляризации моноцитов / макрофагов у больных атеросклерозом
Кл.слова (ненормированные):
Monocytes -- Macrophages -- Cell morphology -- Polyhydroxyalkanoates -- Atherosclerosis -- Intravascular stenting
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients.

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Держатели документа:
Siberian Federal University, 79, Svobodny av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Research Center Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Federal Center for Cardiovascular Surgery, 45 Karaulnaya, Krasnoyarsk, 660020, Russian Federation

Доп.точки доступа:
Menzyanova, N. G.; Pyatina, S. А.; Nikolaeva, E. D.; Shabanov, A. V.; Шабанов, Александр Васильевич; Nemtsev, I. V.; Stolyarov, D. P.; Dryganov, D. B.; Sakhnov, E. V.; Shishatskaya, E. I.
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    Morphological aspects of monocyte/macrophage polarization on biopolymer scaffolds in atherosclerosis patients / Menzyanova [et al.] // J. Biotechnol. Biomater. - 2018. - Vol. 8, Is. 3. - Ст. 1000284, DOI 10.4172/2155-952X.1000284. - Cited References: 67. - The study was supported by the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505). . - ISSN 2155-952X
   Перевод заглавия: Морфологические аспекты поляризации моноцитов / макрофагов на биополимерных каркасах у пациентов с атеросклерозом
Кл.слова (ненормированные):
Monocytes -- Macrophages -- Cell morphology -- Polyhydroxyalkanoates -- PHA -- Copolymers -- Atherosclerosis -- Intravascular stenting
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured substrates, made from polyhydroxyalcanoates (PHAs) of various monomer compositions, prepared by casting polymer solutions on a defatted glass and followed by the solvent evaporation. Its surface relief, being further in direct contact with human cells in vitro was analyzed by AFM and SEM scanning. It was shown, that the features of micro/nano relief depend on the monomeric composition of polymer substrates. Monocytes (MNs) of patients with atherosclerosis and cardiac ischemia undergoing stenting and conventional anti-atherosclerotic therapy were harvested prior to and after stenting. After isolation MNs were cultured and transformed into MPh in direct contact with biopolymer culture substrates with different monomer composition and Nano-reliefs in comparison with culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described and their ratios for five samples of polymers were counted as an intermediate result in the development of “smart” material for blood vessel recovery. The results obtained allow us to assume that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates but also on the initial state of MNs in vivo and the general response of patients.

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Держатели документа:
Siberian Federal University, Svobodny, Krasnoyarsk, Russia
L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russia
Federal Research Centre Krasnoyarsk Scientific Centre of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russia
Federal Centres for Cardiovascular Surgery, Krasnoyarsk, Russia

Доп.точки доступа:
Menzyanova, Natalia G.; Pyatina, Svetlana V.; Nikolaeva, Elena D.; Shabanov, A. V.; Шабанов, Александр Васильевич; Nemtsev, I. V.; Stolyarov, D. P.; Dryganov, Dmitry B.; Sakhnov, Eugene V.; Vinokurova, Daria A.; Shishatskaya, Ekaterina I.
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