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    Ratio fluorescent hybrid probe for visualized fluorescence detection of H2O2 in vitro and in vivo / R. Dong, Y. Yao, D. Li [et al.] // Sens. Actuators B: Chem. - 2020. - Vol. 321. - Ст. 128643, DOI 10.1016/j.snb.2020.128643. - Cited References: 65. - The present work was supported by the National Natural Science Foundations of China (Grant No. 21671070 ), the project supported by the GDUPS (2018) for Prof. Bingfu LEI, the Project for Construction of High-level University in Guangdong Province of China, the Guangzhou Science & Technology Project , China (No. 201707010033 ), the Special Funds for the Cultivation of Guangdong College Students’ Scientific and Technological Innovation (No. 201910564035 ), and the National Undergraduate Innovation and Entrepreneurship Training Program granted for Riyue Dong . - ISSN 0925-4005
   Перевод заглавия: Соотношение флуоресценции в гибридных зондах для визуального детектирования флуоресценции H2O2 in vitro и in vivo
Кл.слова (ненормированные):
Si-O -- QDs -- Ag -- Nanoclusters -- Visualized fluorescence detection -- H2O2 sensing in vivo and in vitro
Аннотация: A silicon oxide quantum dots (Si-O QDs) and Ag nanoclusters hybrid nanosensing probe with dual-emission and small nanocrystals that acts as an “off-on” ratio fluorescent probe for hydrogen peroxide (H2O2) detection was developed. The probe was used to measure the H2O2 concentration generated by mitochondria in vitro via recording the I455/I680 fluorescence ratio. Furthermore, this hybrid probe was applied to monitor the wound-induced H2O2 in lettuce leaf, and realized the visualized fluorescence qualitative detection H2O2 in vivo via laser scanning confocal microscope. The working mechanism of the probe is also investigated. Inner filter effect (IFE) estimation, Fourier transform infrared (FTIR) spectra and high-resolution transmission electron microscopy (HRTEM) images were applied to study the quenching mechanism and recovering reason of Si-O QDs fluorescence. The results show that the blue fluorescence of Si-O QDs can be quenched by Ag NCs via the IFE and fluorescent resonance energy transfer (FRET) effect. After adding H2O2, the surface groups of Ag NCs was changed and the IFE and FRET effects between the Si-O QDs and Ag NCs are disabled, thus the quenched Si-O QDs luminescence can be regularly recovered.

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Держатели документа:
College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China
Guangdong Laboratory of Lingnan Modern Agriculture, Guangzhou, 510642, China
Guangdong Provincial Engineering Technology Research Center for Optical Agriculture, Guangzhou, 510642, China
SB RAS, Kirensky Inst Phys, Lab Crystal Phys, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Dong, R.; Yao, Y.; Li, D.; Zhang, H.; Li, W.; Molokeev, M. S.; Молокеев, Максим Сергеевич; Liu, Y.; Lei, B.
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