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Magnetic and magnetooptical properties of magnetic nanoparticles and DNA-Aptamers conjugates for medical application / A. E. Sokolov [et al.] // VI Euro-Asian Symposium "Trends in MAGnetism" (EASTMAG-2016) : abstracts / ed.: O. A. Maksimova, R. D. Ivantsov. - Krasnoyarsk : KIP RAS SB, 2016. - Ст. P12.4. - P. 561. - The work was supported by the Ministry of Education and Science (Agreement No.14.607.21.0104 (RFMEFI60714X0104)) . - ISBN 978-5-904603-06-9
Кл.слова (ненормированные):
magnetic nanoparticles -- magnetization -- natural and magnetic circular dichroism -- aptamers -- bionanoconjugates

Доп.точки доступа:
Sokolov, A. E.; Соколов, Алексей Эдуардович; Ivanova, O. S.; Иванова, Оксана Станиславовна; Zabluda, V. N.; Заблуда, Владимир Николаевич; Dubynina, A. V.; Дубынина, А. В.; Semina, P. N.; Семина, Полина Николаевна; Zamay, G. S.; Замай, Г. С.; Zamay, T. N.; Замай, Татьяна Н.; Zamay, A. S.; Замай, Анна Сергеевна; Volochaev, M. N.; Волочаев, Михаил Николаевич; Svetlichnyi, V.; Светличный, Валерий; Lapin, I. N.; Лапин, И. Н.; Shabalina, A.; Шабалина, Анастасия; Euro-Asian Symposium "Trends in MAGnetism"(6 ; 2016 ; Aug. ; 15-19 ; Krasnoyarsk); "Trends in MAGnetism", Euro-Asian Symposium(6 ; 2016 ; Aug. ; 15-19 ; Krasnoyarsk); Институт физики им. Л.В. Киренского Сибирского отделения РАН

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Selection of DNA aptamers for breast cancer / G. S. Zamay, I. V. Belyanina, A. S. Zamay [et al.] // Biochem. Mosc.-Suppl. Ser. B-Biomed. Chem. - 2016. - Vol. 10, Is. 2. - P. 158-164, DOI 10.1134/S1990750816020128. - Cited References:23 . - ISSN 1990-7508. - ISSN 1990-7516

РУБ Biochemistry & Molecular Biology
Рубрики:
BIOMARKERS
MORTALITY
PATTERNS
Кл.слова (ненормированные):
SELEX -- DNA aptamers -- oligonucleotides -- breast cancer
Аннотация: A method of selection of DNA aptamers to breast tumor tissue based on the use of postoperative material has been developed. Breast cancer tissues were used as the positive target; the negative targets included benign tumor tissue, adjacent healthy tissues, breast tissues from mastopathy patients, and also tissues of other types of malignant tumors. During selection a pool of DNA aptamers demonstrating selective binding to breast cancer cells and tissues and insignificant binding to breast benign tissues has been obtained. These DNA aptamers can be used for identification of protein markers, breast cancer diagnostics, and targeted delivery of anticancer drugs.

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Публикация на русском языке Селекция ДНК-аптамеров для диагностики рака молочной железы [Текст] / Г. С. Замай [и др.] // Биомедицинская химия. - Москва : Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича, 2016. - Т. 62 № 4. - С. 411-417

Держатели документа:
Kirensky Inst Phys, Krasnoyarsk, Russia.
Voino Yasenetski Krasnoyarsk State Med Univ, Ul Partizana Zheleznyaka 1, Krasnoyarsk 660022, Russia.
KKRCCC, Krasnoyarsk, Russia

Доп.точки доступа:
Zamay, G. S.; Замай, Г. С.; Belyanina, I. V.; Zamay, A. S.; Замай, Анна Сергеевна; Komarova, M. A.; Krat, A. V.; Eremina, E. N.; Zukov, R. A.; Sokolov, A. Е.; Соколов, Алексей Эдуардович; Zamay, T. N.; Замай, Т. Н.
}
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Magnetic nanoparticles and DNA-aptamers conjugates for diagnostics and therapy of cancer : Invited / A. E. Sokolov [et al.] // International workshop on actual problems of condensed matter physics : Program. Book of abstracts / Fed. Res. Center KSC SB RAS, Kirensky Inst. of phys., Sib. Fed. Univ. - Krasnoyarsk, 2017. - P. 13

Материалы совещания

Доп.точки доступа:
Ovchinnikov, S. G. \предс. прогр. ком.\; Овчинников, Сергей Геннадьевич; Sokolov, A. Е.; Соколов, Алексей Эдуардович; Zamay, S. S.; Замай С. С.; Zamay, T. ; Замай Татьяна; Svetlichnyi, V.; Светличный В.; Velikanov, D. A.; Великанов, Дмитрий Анатольевич; Federal Research Center KSC SB RAS; Kirensky Institute of Physics; Siberian Federal Univercity; International Workshop on Actual Problems of Condensed Matter Physics(27 Mar. - 1 Apr. 2017 ; Krasnoyarsk / Cheremushki)
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    International workshop on actual problems of condensed matter physics : Program. Book of abstracts / Fed. Res. Center KSC SB RAS, Kirensky Inst. of phys., Sib. Fed. Univ. ; предс. прогр. ком. S. G. Ovchinnikov. - Krasnoyarsk : [s. n.], 2017. - 30 p.
    Содержание:
Bondarev, I. A. Magnetic and transport properties of the epitaxial Fe3Si film on a Si substrate / I. A. Bondarev. - P .25
Yakovlev, I. A. The magnetic anisotropy of the Fe and Fe(1-x)Si(x) thin films depend on / I. A. Yakovlev [и др.]. - P .12
Другие авторы: Belyaev B. A., Rautskii M. V., Tarasov, I. A., Varnakov S. N, Ovchinnikov, S. G.
Popkov, S. I. Inverted opals as the Josephson networks of weak links : Invited / S. I. Popkov [и др.]. - P .24
Другие авторы: Gokhfeld D. M., Bykov A., Mistonov A., Shabanov A., Terentiev K.
Nikolaev, S. Electronic structure and Fermi surface within the cluster perturbation theory in X-operators representation : Invited / S. Nikolaev, V. I. Kuz'min, S. G. Ovchinnikov. - P .27
Fedorov, A. S. DFT investigation of electronic and optical magnetic properties of one dimensional transition metal halide structuresTmHaI3 : Invited / A. S. Fedorov [и др.]. - P .20
Другие авторы: Kuzubov A. A., Kovaleva E. A., Popova M. I., Kholtobina A. S., Mikhaleva N. S., Visotin M. A.
Ovchinnikov, S. G. Effect of interatomic exchange interaction on spin crossover and Mott-Hubbard transition under high pressure and the physical properties of the low Earth’s mantle : Invited / S. G. Ovchinnikov [и др.]. - P .26
Другие авторы: Orlov Yu. S., Nikolaev S., Nesterov A., Ovchinnikova T.
Tarasov, I. A. Extremely high magnetic-field sensitivity of charge transport in the Mn/SiO2/p-Si hybrid structure / I. A. Tarasov [и др.]. - P .21
Другие авторы: Smolyakov D. A., Rautskii M. V., Lukyanenko A. V., Yakovlev I. A., Ovchinnikov S. G., Volkov N. V.
Volkov, N. V. Marnetic-field sensitivity of charge transport in silicon-based hybrid structures : Invited / N. V. Volkov [et al.]. - P .10
Другие авторы: Tarasov A. S., Rautskii M. V., Lukyanenko A. V., Varnakov S. N., Bondarev I. A., Ovchinnikov S. G.
Lukyanenko, A. V. Fabrication of multi-terminal planar devices based on epitaxial Fe1-xSix films grown on Si(111) / A. V. Lukyanenko, A. S. Tarasov, I. A. Tarasov [et al.] ; A. V. Luyanenko [и др.]. - P .28
Другие авторы: Tarasov A. S., Tarasov I. A., Bondarev I. A., Smolyarova T. E., Yakovlev I. A., Volochaev M. N., Varnakov S. N., Ovchinnikov S. G., Volkov N. V.
Rautskii, M. V. Magnetic field-driven lateral photovoltaic effect in the Fe/SiO2/p-Si hibrid structure with the Scottky barrier / M. V. Rautskii [и др.]. - P .29
Другие авторы: Tarasov A. S., Varnakov S. N., Bondarev I. A., Volochaev M. N., Lukyanenko A. V., Volkov N. V.
Moryachkov, R. Small angle X-ray scattering and atomic structure of aptamer biomolecules / R. Moryachkov [и др.]. - P .14-15
Другие авторы: Tomilin F. N., Shchugoreva I., Spiridonova V., Peters G., Zabluda V.
Tarasov, I. A. Iron silicides and pure iron epitaxial and highly-textured nanostructures on silicon: growth and their physical properties : Invited / I. A. Tarasov [и др.]. - P .23
Другие авторы: Visotin M. A., Solovyov L. A., Rautskii M. V., Zhandun, V. S., Nemtsev I. V., Yakovlev I. A., Varnakov S. N, Ovchinnikov, S. G.
Sokolov, A. E. Magnetic nanoparticles and DNA-aptamers conjugates for diagnostics and therapy of cancer : Invited / A. E. Sokolov [и др.]. - P .13
Другие авторы: Zamay S., Zamay T., Svetlichnyi V., Velikanov D.
Sandalov, I. S. The microscopic origin of ferromagnetism in Fe silicides : Invited / I. S. Sandalov [и др.]. - P .16
Другие авторы: Zamkova N. G., Zhandun V. S., Ovchinnikov, S. G.

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Держатели документа:
Институт физики им. Л.В. Киренского СО РАН

Доп.точки доступа:
Ovchinnikov, S. G. \предс. прогр. ком.\; Овчинников, Сергей Геннадьевич; Lukyanenko, A. V.; Varnakov, S. N.; Bondarev, I. A.; Ovchinnikov, S. G.; Tarasov, I. A.; Svetlichnyi, V.; Velikanov, D. A.; Spiridonova, V.; Peters, G.; Zabluda, V. N.; Popova, M. I.; Kholtobina, A. S.; Mikhaleva, N. S.; Visotin, M. A.; Yakovlev, I. A.; Volkov, N. V.; Rautskii, M. V.; Zhandun, V. S.; Nemtsev, I. V.; Varnakov, S. N; Mistonov, A.; Shabanov, A. V.; Terentiev, K. Yu.; Nesterov, A.; Ovchinnikova, T.; Smolyarova, T. E.; Volochaev, M. N.; Federal Research Center KSC SB RAS; Kirensky Institute of Physics; Siberian Federal Univercity; International Workshop on Actual Problems of Condensed Matter Physics (27 Mar. - 1 Apr. 2017 ; Krasnoyarsk / Cheremushki)
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    Адресная деструкция злокачественных опухолей биоконъюгатами на основе аптамеров / А. В. Дубынина [и др.] // Сиб. мед. обозрение. - 2016. - Т. 101, № 5. - С. 88-90. - Библиогр.: 2 . - ISSN 1819-9496
   Перевод заглавия: Targeted destruction of malignant tumors by bioconjugates on the base of aptamers
Кл.слова (ненормированные):
аптамеры -- золотые наночастицы -- магнитные микродиски -- гипертермия -- плазмонный -- резонанс -- aptamers -- gold nanoparticles -- magnetic microdiscs -- hyperthermia -- plasmon resonance
Аннотация: Широкое распространение в терапии онкологических заболеваний в последние годы получили методы, основанные на применении различных типов наночастиц. Для повышения адресности Адресная деструкция злокачественных опухолей биоконъюгатами на основе аптамеров воздействия применяют различные молекулы, обладающие высокой специфичностью, одними из которых являются аптамеры. В работе исследовался эффект воздействия на опухоль конъюгатов наночастиц золота с аптамерами при воздействии лазера, а также оценивалась гибель клеток опухоли при использовании конъюгатов магнитных микродисков с аптамерами в условиях переменного магнитного поля. Исследование показало возможность применения исследуемых конъюгатов в качестве средств адресной деструкции опухоли.
Widespread in cancer therapy in recent years have become the methods based on the use of different types of nanoparticles. To improve the targeting of the impact are used various molecules having high specificity, some of them are aptamers. The paper studied the effect of influence on the tumor the conjugates of gold nanoparticles with aptamers at laser exposure, as well as tumor cell death was assessed after using the conjugates of magnetic microdiscs with aptamers in a variable magnetic field. The study showed the possibility of using the studied conjugates as agents of targeted destruction of tumor.

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Держатели документа:
Институт физики СО РАН
ФГАОУ ВО «Сибирский федеральный университет»
ФГБОУ ВО Красноярский государственный медицинский университет имени проф. В. Ф. Войно-Ясенецкого
Центр ядерной медицины Сибирского клинического центра ФМБА России

Доп.точки доступа:
Дубынина, А. В.; Dubynina A. V.; Замай, Т. Н.; Zamay T. N.; Коловская, О. С.; Kolovskaya O. S.; Кичкайло, Анна Сергеевна; Kichkaylo A.S.; Белянина, И. В.; Belyanina I. V.; Чанчикова, Н. Г.; Chanchikova N. G.; Токарев, Н. А.; Tokarev N. A.; Карлова, Е. А.; Karlova E. A.; Александровский, Александр Сергеевич; Aleksandrovskiy, A. S.; Шепелевич, Н. В.; Shepelevich N. V.; Озерская, А. В.; Ozerskaya A.V.; Бадрин, Е. А.; Badrin E. A.; Белугин, К. В.; Belugin K. V.; Белкин, С. А.; Belkin S. A.; Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого
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Magnetic nanoparticles and DNA-aptamers conjugates for cancer therapy / A. Е. Sokolov [et al.] // Int. Baltic Conf. Magnet. (IBCM-2017) : Focus on funcshionalized magnetic struct. for energy and biotech. : book of abstracts. - 2017. - P. 77. - Библиогр.: 4

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Доп.точки доступа:
Sokolov, A. Е.; Соколов, Алексей Эдуардович; Ivanova, O. S.; Иванова, Оксана Станиславовна; Zabluda, V. N.; Заблуда, Владимир Николаевич; Dubinina, A. V.; Semina, P. N.; Семина, Полина Николаевна; Zamay, G. S.; Замай Г. С.; Zamay, T. N.; Zamay, S. S.; Замай С. С.; Volochaev, M. N.; Волочаев, Михаил Николаевич; Svetlichnyi, V.; Светличный В.; Lapin, I. N.; Лапин Иван; Shabalina, A.; Шабалина Анастасия; Solodova, O. V.; Солодова О. В.; International Baltic Conference on Magnetism: Focus on funcshionalized magnetic structures for energy and biotechnology (2 ; 2017 ; 20-24 Aug. ; Svetlogorsk); Балтийский федеральный университет им. И. Канта
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In vivo cancer cells elimination guided by aptamer-functionalized gold-coated magnetic nanoparticles and controlled with low frequency alternating magnetic field / I. V. Belyanina [et al.] // Theranostics. - 2017. - Vol. 7, Is. 13. - P. 3326-3337, DOI 10.7150/thno.17089. - Cited References:35. - The authors are grateful to George Y. Vorogeikin, Yuri I. Vorogeikin and "OKB ART". Andrey Barinov and "OPTEC Group" for help with 3D laser scanning imaging. Microscopic analyses using Carl Zeiss LSM 800 were done in the "Center for bioassay, nanotechnology and nanomaterials safety" ("Biotest-Nano") (Multiple-Access Center, Tomsk State University, Tomsk, Russia). Toxicity studies have been performed in Multiple-Access Center, Central Scientific Research Laboratory in Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecky. This work was supported by the Russian Scientific Fund (grant #14-15-00805). . - ISSN 1838-7640

РУБ Medicine, Research & Experimental
Рубрики:
PHOTOTHERMAL THERAPY
   INTEGRIN ACTIVATION
   FIBRONECTIN
   STIMULATION
Кл.слова (ненормированные):
cancer therapy -- gold coated magnetic nanoparticles -- DNA aptamers -- low -- frequency alternating magnetic field -- fibronectin -- integrin -- apoptosis -- necrosis
Аннотация: Biomedical applications of magnetic nanoparticles under the influence of a magnetic field have been proved useful beyond expectations in cancer therapy. Magnetic nanoparticles are effective heat mediators, drug nanocarriers, and contrast agents; various strategies have been suggested to selectively target tumor cancer cells. Our study presents magnetodynamic nanotherapy using DNA aptamer-functionalized 50 nm gold-coated magnetic nanoparticles exposed to a low frequency alternating magnetic field for selective elimination of tumor cells in vivo. The cell specific DNA aptamer AS-14 binds to the fibronectin protein in Ehrlich carcinoma hence helps deliver the gold-coated magnetic nanoparticles to the mouse tumor. Applying an alternating magnetic field of 50 Hz at the tumor site causes the nanoparticles to oscillate and pull the fibronectin proteins and integrins to the surface of the cell membrane. This results in apoptosis followed by necrosis of tumor cells without heating the tumor, adjacent healthy cells and tissues. The aptamer-guided nanoparticles and the low frequency alternating magnetic field demonstrates a unique non-invasive nanoscalpel technology for precise cancer surgery at the single cell level.

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Держатели документа:
Krasnoyarsk State Med Univ, Krasnoyarsk, Russia.
Russian Acad Sci, KSC Siberian Branch, Fed Res Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Univ Ottawa, Dept Chem & Biomol Sci, Ottawa, ON, Canada.
Inst Computat Modeling RAS SB, Krasnoyarsk, Russia.

Доп.точки доступа:
Belyanina, I. V.; Zamay, T. N.; Замай Т. Н.; Zamay, G. S.; Замай, Галина Сергеевна; Zamay, S. S.; Замай С. С.; Kolovskaya, Olga S.; Ivanchenko, Tatiana I.; Denisenko, Valery V.; Kirichenko, Andrey K.; Glazyrin, Yury E.; Garanzha, Irina V.; Grigorieva, Valentina V.; Shabanov, A. V.; Шабанов, Александр Васильевич; Veprintsev, Dmitry V.; Sokolov, A. E.; Соколов, Алексей Эдуардович; Sadovskii, Vladimir M.; Gargaun, Ana; Berezovski, M. V.; Kichkailo, Anna S.; Russian Scientific Fund [14-15-00805]
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Four steps for revealing and adjusting the 3D structure of aptamers in solution by small-angle X-ray scattering and computer simulation / F. N. Tomilin [et al.] // Anal. Bioanal. Chem. - 2019. - Vol. 411, Is. 25. - P. 6723-6732, DOI 10.1007/s00216-019-02045-0. - Cited References: 51. - Authors are grateful to Ana Gargaun for English grammar correction. This work was funded in parts by the Ministry of Science and Higher Education of the Russian Federation; project 0287-2019-0007 the Council of the President of the Russian Federation for Support of Young Scientists and Leading Scientific Schools (project no. SP-938.2015.5) and the grant of KSAI “Krasnoyarsk Regional Fund of Supporting Scientific and Technological Activities” for M.P., the internship “The study of the stacking of the secondary structure of DNA aptamers to thrombin” for R.M. . - ISSN 1618-2642
Кл.слова (ненормированные):
Aptamer -- Thrombin -- Three-dimensional structure -- Small-angle X-ray scattering -- Molecular modeling
Аннотация: Nucleic acid (NA) aptamers bind to their targets with high affinity and selectivity. The three-dimensional (3D) structures of aptamers play a major role in these non-covalent interactions. Here, we use a four-step approach to determine a true 3D structure of aptamers in solution using small-angle X-ray scattering (SAXS) and molecular structure restoration (MSR). The approach consists of (i) acquiring SAXS experimental data of an aptamer in solution, (ii) building a spatial distribution of the molecule’s electron density using SAXS results, (iii) constructing a 3D model of the aptamer from its nucleotide primary sequence and secondary structure, and (iv) comparing and refining the modeled 3D structures with the experimental SAXS model. In the proof-of-principle we analyzed the 3D structure of RE31 aptamer to thrombin in a native free state at different temperatures and validated it by circular dichroism (CD). The resulting 3D structure of RE31 has the most energetically favorable conformation and the same elements such as a B-form duplex, non-complementary region, and two G-quartets which were previously reported by X-ray diffraction (XRD) from a single crystal. More broadly, this study demonstrates the complementary approach for constructing and adjusting the 3D structures of aptamers, DNAzymes, and ribozymes in solution, and could supply new opportunities for developing functional nucleic acids. [Figure not available: see fulltext.]. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

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Держатели документа:
Kirensky Institute of Physics, Federal Research Center KSC Siberian Branch Russian Academy of Sciences, 50/38 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, 79 Svobodny pr., Krasnoyarsk, 660041, Russian Federation
Federal Research Center “Krasnoyarsk Science Center” Siberian Branch of the Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
NRC Kurchatov Institute, 1, Academic Kurchatov Square, Moscow, 123182, Russian Federation
A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1/40 Leninskie Gory, Moscow, 119992, Russian Federation
Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk, 660022, Russian Federation
Department of Chemistry and Biomolecular Sciences, University of Ottawa, 10 Marie-Curie, Ottawa, ON K1N6N5, Canada

Доп.точки доступа:
Tomilin, F. N.; Томилин, Феликс Николаевич; Moryachkov, R.; Морячков, Роман Владимирович; Shchugoreva, I.; Zabluda, V. N.; Заблуда, Владимир Николаевич; Peters, G.; Platunov, M. S.; Платунов, Михаил Сергеевич; Spiridonova, V.; Melnichuk, A.; Atrokhova, A.; Zamay, S. S.; Замай, С. С.; Ovchinnikov, S. G.; Овчинников, Сергей Геннадьевич; Zamay, G. S.; Замай, Галина Сергеевна; Sokolov, A. Е.; Соколов, Алексей Эдуардович; Zamay, T. N.; Замай, Т. Н.; Berezovski, M. V.; Kichkailo, A. S.
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Aptamer-conjugated superparamagnetic ferroarabinogalactan nanoparticles for targeted magnetodynamic therapy of cancer / O. S. Kolovskaya, T. N. Zamay, G. S. Zamay [et al.] // Cancers. - 2020. - Vol. 12, Is. 1. - Ст. 216, DOI 10.3390/cancers12010216. - Cited References: 46. - This research was funded by the Ministry of Science and Higher Education of the Russian Federation; project 0287-2019-0007 . - ISSN 2072-6694
Кл.слова (ненормированные):
aptamers -- arabinogalactan -- superparamagnetic ferroarabinogalactans -- drug delivery -- magnetodynamic therapy -- magnetically induced cell disruption -- magnetic resonance imaging
Аннотация: Nanotechnologies involving physical methods of tumor destruction using functional oligonucleotides are promising for targeted cancer therapy. Our study presents magnetodynamic therapy for selective elimination of tumor cells in vivo using DNA aptamer-functionalized magnetic nanoparticles exposed to a low frequency alternating magnetic field. We developed an enhanced targeting approach of cancer cells with aptamers and arabinogalactan. Aptamers to fibronectin (AS-14) and heat shock cognate 71 kDa protein (AS-42) facilitated the delivery of the nanoparticles to Ehrlich carcinoma cells, and arabinogalactan (AG) promoted internalization through asialoglycoprotein receptors. Specific delivery of the aptamer-modified FeAG nanoparticles to the tumor site was confirmed by magnetic resonance imaging (MRI). After the following treatment with a low frequency alternating magnetic field, AS-FeAG caused cancer cell death in vitro and tumor reduction in vivo. Histological analyses showed mechanical disruption of tumor tissues, total necrosis, cell lysis, and disruption of the extracellular matrix. The enhanced targeted magnetic theranostics with the aptamer conjugated superparamagnetic ferroarabinogalactans opens up a new venue for making biocompatible contrasting agents for MRI imaging and performing non-invasive anti-cancer therapies with a deep penetrated magnetic field.

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Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science”, 660036 Krasnoyarsk, Russia
Laboratory for Biomolecular and Medical Technologies, Faculty of Medicine, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, 660022 Krasnoyarsk, Russia
Irkutsk Institute of Chemistry named after A.E. Favorsky, the Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, Russia
L.V. Kirensky Institute of Physics SB RAS—The Branch of Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, 660036 Krasnoyarsk, Russia
Laboratory of Advanced Materials and Technology, Tomsk State University, 634050 Tomsk, Russia
Institute of Chemistry and Chemical Technology SB RAS—The Branch of Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, 660036 Krasnoyarsk, Russia
School of Engineering Physics and Radio Electronics, Siberian Federal University, 660041 Krasnoyarsk, Russia
Research Center for Computational Design of Advanced Functional Materials (CD-FMat), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan
School of Non-Ferrous Metals and Materials Science, Siberian Federal University, 660041 Krasnoyarsk, Russia
Faculty of Physics, Department of Magnetism, Lomonosov Moscow State University, 119991 Moscow, Russia
School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia
Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada

Доп.точки доступа:
Kolovskaya, O. S.; Коловская, О. С.; Zamay, T. N.; Замай, Т. Н.; Zamay, G. S.; Замай, Галина Сергеевна; Babkin, V. A.; Medvedeva, E. N.; Neverova, N. A.; Kirichenko, A. K.; Zamay, S. S.; Замай, С. С.; Lapin, I. N.; Morozov, E. V.; Морозов, Евгений Владимирович; Sokolov, A. Е.; Соколов, Алексей Эдуардович; Narodov, A. A.; Fedorov, D. G.; Tomilin, F. N.; Томилин, Феликс Николаевич; Zabluda, V. N.; Заблуда, Владимир Николаевич; Alekhina, Yu.; Lukyanenko, K. A.; Glazyrin, Yu. E.; Svetlichnyi, V. A.; Berezovski, M. V.; Kichkailo, A. S.
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10.

Molecular epitope determination of aptamer complexes of the multidomain protein C-met by proteolytic affinity-mass spectrometry / L. Lupu, P. Wiegand, N. Huttmann [et al.] // ChemMedChem. - 2020. - Vol. 15, Is. 4. - P. 363-369, DOI 10.1002/cmdc.201900489. - Cited References: 40. - We gratefully acknowledge the advice and assistance of Prof. Friedemann Volklein and Oliver Muller, MSc in the preparation of chips for the SPR affinity determinations. We thank Dr. Stefan Maeser, Biogen GmbH, Munchen, for valuable advice and critical reading of the manuscript. This work has been partially funded (Chip-MS epitope analysis) by the LOEWE-3 Funding Agency, Hessen-Agentur, Wiesbaden, Germany; Grant 696/19-16 . - ISSN 1860-7179. - ISSN 1860-7187

РУБ Chemistry, Medicinal + Pharmacology & Pharmacy
Рубрики:
DNA APTAMERS
ANTIBODIES
RECOGNITION
Кл.слова (ненормированные):
C-Met protein -- tumor biomarkers -- aptamer-C-Met complexes -- aptamer epitopes -- affinity-mass spectrometry -- epitope peptide analysis
Аннотация: C‐Met protein is a glycosylated receptor tyrosine kinase of the hepatocyte growth factor (HGF), composed of an α and a β chain. Upon ligand binding, C‐Met transmits intracellular signals by a unique multi‐substrate docking site. C‐Met can be aberrantly activated leading to tumorigenesis and other diseases, and has been recognized as a biomarker in cancer diagnosis. C‐Met aptamers have been recently considered a useful tool for detection of cancer biomarkers. Herein we report a molecular interaction study of human C‐Met expressed in kidney cells with two DNA aptamers of 60 and 64 bases (CLN0003 and CLN0004), obtained using the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure. Epitope peptides of aptamer‐C‐Met complexes were identified by proteolytic affinity‐mass spectrometry in combination with SPR biosensor analysis (PROTEX‐SPR‐MS), using high‐pressure proteolysis for efficient digestion. High affinities (KD, 80–510 nM) were determined for aptamer‐C‐Met complexes, with two‐step binding suggested by kinetic analysis. A linear epitope, C‐Met (381–393) was identified for CLN0004, while the CLN0003 aptamer revealed an assembled epitope comprised of two peptide sequences, C‐Met (524–543) and C‐Met (557–568). Structure modeling of C‐Met‐aptamers were consistent with the identified epitopes. Specificities and affinities were ascertained by SPR analysis of the synthetic epitope peptides. The high affinities of aptamers to C‐Met, and the specific epitopes revealed render them of high interest for cellular diagnostic studies.

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Держатели документа:
Steinbeis Ctr Biopolymer Anal & Biomed Mass Spect, Marktstr 29, D-65428 Russelsheim, Germany.
Univ Ottawa, Dept Chem & Biomol Sci, Ottawa, ON K1N 6N5, Canada.
Rhein Main Univ, Dept Engn Sci, D-65428 Russelsheim, Germany.
Russian Acad Sci, Siberian Branch, Kirensky Inst Phys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 66041, Russia.
Russian Acad Sci, Fed Res Ctr, Lab Digital Controlled Drugs & Theranost, Siberian Branch,Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.
Pressure Biosci Inc, 14 Norfolk Ave, South Easton, MA 02375 USA.

Доп.точки доступа:
Lupu, Loredana; Wiegand, Pascal; Huttmann, N.; Rawer, Stephan; Kleinekofort, Wolfgang; Shugureva, Irina; Kichkailo, Anna S.; Tomilin, F. N.; Томилин, Феликс Николаевич; Lazarev, Alexander; Berezovski, Maxim V.; Przybylski, Michael; LOEWE-3 Funding Agency, Hessen-Agentur, Wiesbaden, Germany [696/19-16]
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