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Electrochemical aptasensor for lung cancer-related protein detection in crude blood plasma samples / G. S. Zamay [et al.] // Sci. Rep. - 2016. - Vol. 6. - Ст. 34350, DOI 10.1038/srep34350. - Cited References:24. - Authors thank Dr. Mahmoud Labib and Galina Kudryasheva. This work was supported by Ministry of Education and Science of Russian Federation Federal Target Program #14.604.21.0105 for Anna S. Zamay. Electron microscopy was carried out at the Multiple-Access Center of Krasnoyarsk Research Center Siberian branch of Russian Academy of Science. . - ISSN 2045-2322

РУБ Multidisciplinary Sciences
Рубрики:
SQUARE-WAVE VOLTAMMETRY
   GENE-EXPRESSION
   SERUM
   BIOSENSORS
   DIAGNOSIS
   ADENOCARCINOMA
   BIOMARKERS
   SENSOR
Аннотация: The development of an aptamer-based electrochemical sensor for lung cancer detection is presented in this work. A highly specific DNA-aptamer, LC-18, selected to postoperative lung cancer tissues was immobilized onto a gold microelectrode and electrochemical measurements were performed in a solution containing the redox marker ferrocyanide/ferricyanide. The aptamer protein targets were harvested from blood plasma of lung cancer patients by using streptavidin paramagnetic beads and square wave voltammetry of the samples was performed at various concentrations. In order to enhance the sensitivity of the aptasensor, silica-coated iron oxide magnetic beads grafted with hydrophobic C8 and C4 alkyl groups were used in a sandwich detection approach. Addition of hydrophobic beads increased the detection limit by 100 times. The detection limit of the LC-18 aptasensor was enhanced by the beads to 0.023 ng/mL. The formation of the aptamer -protein -bead sandwich on the electrode surface was visualized by electron microcopy. As a result, the electrochemical aptasensor was able to detect cancer-related targets in crude blood plasma of lung cancer patients.

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Держатели документа:
Krasnoyarsk State Med Univ, Lab Biomol & Med Technol, 1 P Zheleznyaka, Krasnoyarsk 660022, Russia.
Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, 50-24 Akademgorodok, Krasnoyarsk 660036, Russia.
Design Dept Iskra, 1 Televizornaya, Krasnoyarsk 660028, Russia.
Russian Acad Sci, Siberian Branch, Krasnoyarsk Res Ctr, 50 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Reg Clin Canc Ctr, 1 Smolenskaya, Krasnoyarsk 660022, Russia.
Univ Ottawa, Dept Chem, 10 Marie Curie,DIorio Hall,Room 201, Ottawa, ON K1N 6N5, Canada.
Russian Acad Sci, Siberian Branch, Inst Phys, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zamay, G. S.; Zamay, T. N.; Kolovskii, V. A.; Shabanov, A. V.; Шабанов, Александр Васильевич; Glazyrin, Y. E.; Veprintsev, D. V.; Krat, A. V.; Zamay, S. S.; Kolovskaya, O. S.; Gargaun, A.; Sokolov, A. E.; Соколов, Алексей Эдуардович; Modestov, A. A.; Artyukhov, I. P.; Chesnokov, N. V.; Petrova, M. M.; Berezovski, M. V.; Zamay, A. S.; Ministry of Education and Science of Russian Federation Federal Target Program [14.604.21.0105]
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Selection of DNA aptamers for breast cancer / G. S. Zamay, I. V. Belyanina, A. S. Zamay [et al.] // Biochem. Mosc.-Suppl. Ser. B-Biomed. Chem. - 2016. - Vol. 10, Is. 2. - P. 158-164, DOI 10.1134/S1990750816020128. - Cited References:23 . - ISSN 1990-7508. - ISSN 1990-7516

РУБ Biochemistry & Molecular Biology
Рубрики:
BIOMARKERS
MORTALITY
PATTERNS
Кл.слова (ненормированные):
SELEX -- DNA aptamers -- oligonucleotides -- breast cancer
Аннотация: A method of selection of DNA aptamers to breast tumor tissue based on the use of postoperative material has been developed. Breast cancer tissues were used as the positive target; the negative targets included benign tumor tissue, adjacent healthy tissues, breast tissues from mastopathy patients, and also tissues of other types of malignant tumors. During selection a pool of DNA aptamers demonstrating selective binding to breast cancer cells and tissues and insignificant binding to breast benign tissues has been obtained. These DNA aptamers can be used for identification of protein markers, breast cancer diagnostics, and targeted delivery of anticancer drugs.

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Публикация на русском языке Селекция ДНК-аптамеров для диагностики рака молочной железы [Текст] / Г. С. Замай [и др.] // Биомедицинская химия. - Москва : Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича, 2016. - Т. 62 № 4. - С. 411-417

Держатели документа:
Kirensky Inst Phys, Krasnoyarsk, Russia.
Voino Yasenetski Krasnoyarsk State Med Univ, Ul Partizana Zheleznyaka 1, Krasnoyarsk 660022, Russia.
KKRCCC, Krasnoyarsk, Russia

Доп.точки доступа:
Zamay, G. S.; Замай, Г. С.; Belyanina, I. V.; Zamay, A. S.; Замай, Анна Сергеевна; Komarova, M. A.; Krat, A. V.; Eremina, E. N.; Zukov, R. A.; Sokolov, A. Е.; Соколов, Алексей Эдуардович; Zamay, T. N.; Замай, Т. Н.
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