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Short-term culture of monocytes as an in vitro evaluation system for bionanomaterials designated for medical use / E. I. Shishatskaya [et al.] // Food Chem. Toxicol. - 2016. - Vol. 96. - P. 302-308, DOI 10.1016/j.fct.2016.08.025. - Cited References: 46 . - ISSN 0278-6915

РУБ Food Science & Technology + Toxicology
Рубрики:
MAGNETIC NANOPARTICLES
   CELLULAR REDUCTION
   PROTEIN CORONA
   CELLS
   MECHANOTRANSDUCTION
   NANOMATERIALS
   LOCALIZATION
   BIOMATERIALS
   CYTOSKELETON
   ACTIVATION
Кл.слова (ненормированные):
Biocompatible biopolymers -- Polyhydroxyalkanoates -- Nanomaterials -- Nanodiamonds -- Fullerenes -- Bio-nanomaterials -- Monocytes
Аннотация: We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the viability/metabolic activity of cells. The scanning electron microscopy assay can detect fine changes in cell morphology. In the present study BNM have been shown to affect; in a size, chemical composition and morphological characteristics-dependent manner, the ability of monocytes to reduce MTT as well as their morphology. Moreover, the specific effects of ND and F on MTT reduction and cell morphology were exhibited in a dose-dependent manner and sensitive to the formation of surface protein corona. Our results suggest that short-term culture of monocytes is a sensitive model system for assessing the biological effects of BMPs in vitro. © 2016 Elsevier Ltd

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Держатели документа:
Siberian Federal University, Svobodnuy Av. 79, Krasnoyarsk, Russian Federation
Laboratory of Anatomy, Histology, Embryology, School of Medicine, University of Crete, Heraklion, Greece
Institute of Physics, Russian Academy of Science, Siberian Division, Akademgorodok, 50, Build. 38, Krasnoyarsk, Russian Federation
Laboratory of Toxicology, Medical School, University of Crete, Heraklion, Greece

Доп.точки доступа:
Shishatskaya, E. I.; Шишацкая, Екатерина Игоревна; Nikitovic, D.; Shabanov, A. V.; Шабанов, Александр Васильевич; Tzanakakis, G. N.; Tsatsakis, A. M.; Menzianova, N. G.
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